CN115520962A - With NO 3- Domestication culture method of electroactive anaerobic ammonium oxidation microorganisms serving as single nitrogen source - Google Patents
With NO 3- Domestication culture method of electroactive anaerobic ammonium oxidation microorganisms serving as single nitrogen source Download PDFInfo
- Publication number
- CN115520962A CN115520962A CN202210783293.XA CN202210783293A CN115520962A CN 115520962 A CN115520962 A CN 115520962A CN 202210783293 A CN202210783293 A CN 202210783293A CN 115520962 A CN115520962 A CN 115520962A
- Authority
- CN
- China
- Prior art keywords
- ammonium oxidation
- anaerobic ammonium
- electroactive
- microorganisms
- anaerobic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims abstract description 184
- 229910052757 nitrogen Inorganic materials 0.000 title claims abstract description 92
- 244000005700 microbiome Species 0.000 title claims abstract description 60
- 230000003647 oxidation Effects 0.000 title claims abstract description 52
- 238000007254 oxidation reaction Methods 0.000 title claims abstract description 52
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 title claims abstract description 50
- 238000012136 culture method Methods 0.000 title claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 238000006243 chemical reaction Methods 0.000 claims abstract description 18
- 230000000694 effects Effects 0.000 claims abstract description 16
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229910002651 NO3 Inorganic materials 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 9
- 239000010865 sewage Substances 0.000 claims abstract description 8
- 239000000758 substrate Substances 0.000 claims abstract description 7
- 239000010802 sludge Substances 0.000 claims description 19
- 238000009825 accumulation Methods 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 9
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 claims description 7
- 239000002245 particle Substances 0.000 claims description 7
- 230000014759 maintenance of location Effects 0.000 claims description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 5
- 229910052799 carbon Inorganic materials 0.000 claims description 5
- 235000003642 hunger Nutrition 0.000 claims description 5
- 230000037361 pathway Effects 0.000 claims description 4
- 230000037351 starvation Effects 0.000 claims description 4
- 235000013619 trace mineral Nutrition 0.000 claims description 4
- 239000011573 trace mineral Substances 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 230000010718 Oxidation Activity Effects 0.000 claims description 3
- 229930182555 Penicillin Natural products 0.000 claims description 3
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims description 3
- 238000005189 flocculation Methods 0.000 claims description 3
- 230000016615 flocculation Effects 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 229940049954 penicillin Drugs 0.000 claims description 3
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 claims description 2
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 2
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 claims description 2
- 238000004062 sedimentation Methods 0.000 claims description 2
- 238000009210 therapy by ultrasound Methods 0.000 claims description 2
- 230000037452 priming Effects 0.000 claims 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract description 26
- 229910021529 ammonia Inorganic materials 0.000 abstract description 13
- 230000005684 electric field Effects 0.000 abstract description 8
- 230000001651 autotrophic effect Effects 0.000 abstract description 5
- 230000037353 metabolic pathway Effects 0.000 abstract description 3
- PDNNQADNLPRFPG-UHFFFAOYSA-N N.[O] Chemical compound N.[O] PDNNQADNLPRFPG-UHFFFAOYSA-N 0.000 abstract 1
- 230000004913 activation Effects 0.000 abstract 1
- 238000012851 eutrophication Methods 0.000 description 4
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 3
- 230000001590 oxidative effect Effects 0.000 description 3
- 239000003054 catalyst Substances 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 239000005431 greenhouse gas Substances 0.000 description 2
- 238000002604 ultrasonography Methods 0.000 description 2
- UOMQUZPKALKDCA-UHFFFAOYSA-K 2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxymethyl)amino]acetate;iron(3+) Chemical compound [Fe+3].OC(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UOMQUZPKALKDCA-UHFFFAOYSA-K 0.000 description 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 1
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 1
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000027756 respiratory electron transport chain Effects 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/005—Combined electrochemical biological processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/163—Nitrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Water Supply & Treatment (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Organic Chemistry (AREA)
- Electrochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Water Treatment By Electricity Or Magnetism (AREA)
Abstract
The invention relates to the field of sewage treatment and provides a method for treating sewage with NO 3 ‑ Is a domestication culture method of an electroactive anaerobic ammonium oxidation microorganism with a single nitrogen source. Firstly, domesticating anaerobic ammonium oxidation microorganisms with electric activity in an external power supply mode, and then domesticating and exciting dissimilatory nitrate of the anaerobic ammonium oxidation microorganisms with the electric activity through a substrate to reduce the dissimilatory nitrate into an ammonia metabolic pathway, thereby realizing the purposes of taking electric field cathode electrons as electron donors and NO as NO 3 ‑ Is anaerobic ammonium oxidation autotrophic denitrification of single nitrogen source. The invention realizes single NO 3 ‑ Anaerobic ammoxidation reaction in nitrogen source mode to reach NO in water 3 ‑ The autotrophic removal of (1), domesticated with NO 3 ‑ An electroactive anammox microorganism as a single nitrogen source for the treatment of NO in water 3 ‑ The direct removal rate of the ammonia oxygen can reach over 75 percent, which is higher than that of anaerobic ammonia oxygenThe activation energy can reach 0.3 g-N/(g) ‑1 ‑VSS·d ‑1 ) The above.
Description
Technical Field
The invention belongs to the technical field of sewage treatment, and relates to a method for treating sewage by NO 3 - Is a domestication culture method of an electroactive anaerobic ammonium oxidation microorganism with a single nitrogen source.
Background
The eutrophication phenomenon of water bodies is still one of the most serious environmental problems in the world, and the anaerobic ammonia oxidation is the sewage biological denitrification technology with the highest popularization and application value. Nitrite nitrogen NO is used by traditional anaerobic ammonia oxidation microorganisms 2 - As electron acceptor, ammonia is oxynitrided to N under oxygen-deficient conditions 2 Thereby realizing the autotrophic removal of nitrogen in the water body and avoiding the consumption of organic carbon source and the generation of greenhouse gas N in the traditional denitrification 2 The problem of O discharge, but for nitrate nitrogen NO stably existing in the water body 3 - Removal is still not directly effective.
Disclosure of Invention
The NO is reduced by electrons provided by an electric field cathode in the anaerobic ammonium oxidation microorganism domesticated to have the electrical activity 3 - Accumulation of NO 2 - And replacing NH by a cathode electron 4 + Status of electron donors, achieving a single NO 3 - Anaerobic ammoxidation reaction in nitrogen source mode to reach NO in water 3 - The autotrophy removal of the water solves the problem of water eutrophication.
The technical scheme of the invention is as follows:
with NO 3 - The domestication and culture method of the electrically active anaerobic ammonium oxidation microorganisms which are single nitrogen sources comprises the following steps:
(1) pretreatment of inoculated sludge: anaerobic ammonium oxidation sludge granular sludge is used as an inoculum, after starvation pretreatment, particle disintegration and flocculation are promoted by ultrasound, and flocculent sludge is inoculated into a cathode reaction chamber of the double-cavity electrolytic cell after sedimentation and concentration for acclimatization and culture.
Further, the particle size of the granular sludge is 1.0-3.0mm, and after 5-15d starvation pretreatment, the particles are accelerated to disintegrate and flocculate by ultrasound for 1 h;
(2) controlling the working condition of domestication and culture of the electroactive anaerobic ammonium oxidation microorganisms: controlling the concentration of flocculent sludge in the cathode reactor at 2500-4000mg/L, adding 0.5% penicillin to inhibit the growth of heterotrophic bacteria, introducing Ar and CO 2 Creating an anaerobic environment, initial NH 4 + With NO 2 - The concentration of the active ingredient is 100mg-N/L and 120mg-N/L respectively, the operation is carried out continuously, the Hydraulic Retention Time (HRT) is 12h, and NaHCO is supplemented every 2d 3 And the microelements are supplied for the growth of the anaerobic ammonium oxidation microorganisms, the load is increased by 25 percent when every 10 days is taken as a period, and the stirring paddle is used for stirring to ensure the uniform mass transfer in the system.
Further, ar and CO 2 Is 95%:5 percent, stirring for 10min every 60min by a stirring paddle to ensure the uniform mass transfer in the system.
NH to be fed into the cathode reactor 4 + With NO 2 - The concentration of the nitrogen reaches 300mg-N/L and 360mg-N/L respectively, and NH is gradually reduced by 25 percent when the total nitrogen removal rate is stabilized to be more than 75 percent 4 + Up to 0, thereby establishing the reduction of NO with cathodic electrons 2 - Generating N 2 The generation of the reaction path domesticates the anaerobic ammonium oxidation microorganism with electric activity.
(3) Nitrate (NO) reduction by activated cathode electron-donating electroactive anammox microorganisms 3 - ) Accumulation of Nitrite (NO) 2 - ) And (3) controlling the working conditions of the paths: in said cathodic reactor, where electroactive microorganisms are successfully domesticated, the substrate composition is altered with NO 3 - As a nitrogen source, 1% of disodium ferric EDTA is added to promote the excitation of DNRA pathway;
initial NO 3 - The concentration is 120mg-N/L, the operation is carried out continuously, the Hydraulic Retention Time (HRT) is 12h, and NaHCO is supplemented every 2d 3 And the trace elements are supplied for the growth of anaerobic ammonium oxidation microorganisms, the load is increased by 25 percent every 10 days as a period until the water inlet NO of the system 3 - The concentration reaches 360mg-N/L,NO 3 - when the removal rate is stabilized to be more than 75 percent, the electro-active anaerobic ammonium oxidation microorganisms are shown to reduce Nitrate (NO) 3 - ) Accumulation of Nitrite (NO) 2 - ) Is excited and further reduces NO using cathode electrons 2 - Generating N 2 Thereby realizing the NO of the electroactive anaerobic ammonium oxidation microorganism in the water body 3 - Effective removal of (i.e. with NO) 3 - The electrically active anaerobic ammonium oxidation microorganism which is a single nitrogen source is successfully domesticated, and the specific anaerobic ammonium oxidation activity can reach 0.3 g-N/(g) -1 -VSS·d -1 ) As described above.
The anaerobic ammonium oxidation microorganism with the electrical activity adopts a double-cavity electrolytic tank as a reaction device, a direct-current stabilized voltage power supply controls the voltage range to be 0.8-1.2V, and domestication and enrichment of the anaerobic ammonium oxidation microorganism with the electrical activity are carried out in a cathode reaction tank.
Further, the double-cavity electrolytic cell comprises a cathode reaction chamber, an anode chamber, a direct current stabilized voltage power supply and a salt bridge, wherein two ends of the salt bridge are respectively inserted into the cathode reaction chamber and the anode chamber, an electric field cathode and a reference electrode of the direct current stabilized voltage power supply are arranged in the cathode reaction chamber, an electric field anode is arranged in the anode chamber, the anode chamber is filled with a KCl buffer solution, the cathode reaction chamber is used for domestication and culture of electroactive anaerobic ammonium oxidation microorganisms, and a stirrer is arranged in the cathode reaction chamber;
by NO as defined above 3 - The method is characterized in that anaerobic ammonium oxidation microorganisms with electric activity are domesticated and cultured by a single nitrogen source and are applied to removal of nitrate nitrogen in sewage, and the method comprises the following steps: firstly domesticating and culturing anaerobic ammonium oxidation microorganisms with electric activity, and then stimulating dissimilatory nitrate of the anaerobic ammonium oxidation microorganisms to be reduced into ammonia (DNRA) metabolic pathway by domesticating substrates to realize the purposes of taking electric field cathode electrons as electron donors and NO as NO 3 - Is the anaerobic ammonia oxidation autotrophic nitrogen removal of a single nitrogen source.
The invention has the beneficial effects that:
the invention domesticates the anaerobic ammonium oxidation microorganism with electric activity, so that the anaerobic ammonium oxidation microorganism utilizes electrons provided by an electric field cathodeReduction of NO 3 Accumulation of NO 2 -, and replacing NH by a cathodic electron 4 + Status of electron donors, achieving a single NO 3 Anaerobic ammoxidation reaction in nitrogen source mode to reach NO in water 3 -autotrophic removal.
The invention uses nitrate Nitrogen (NO) 3 - ) An electroactive anammox microorganism as a single nitrogen source for the removal of NO from water 3 - Avoiding the consumption of organic carbon source and the generation of greenhouse gas N in the traditional denitrification 2 The invention has positive significance for solving the problem of water eutrophication.
The domestication culture method of the electroactive anaerobic ammonium oxidation microorganisms comprises the steps of inoculating sludge pretreatment, controlling the working condition of domestication culture of the electroactive anaerobic ammonium oxidation microorganisms, and exciting cathode electrons to supply the electroactive anaerobic ammonium oxidation microorganisms to reduce Nitrate (NO) 3 -) accumulation of Nitrite (NO) 2 -) three steps of controlling the working condition of the pathway, and matching various parameters, so that the acclimatization successfully uses NO under the synergistic effect 3 - An electroactive anammox microorganism as a single nitrogen source for the treatment of NO in water 3 - The removal rate of the catalyst can reach 75 percent, and the specific ammoxidation activity of the catalyst can reach 0.3 g-N/(g) -1 -VSS·d -1 ) The above.
Drawings
FIG. 1 shows water inlet and outlet indexes and total nitrogen removal rate in the acclimatization stage
FIG. 2 electroactive anammox microorganisms vs. single NO 3 Effect of removal of
Detailed Description
Example 1 with NO 3 - The domestication culture method of the electroactive anaerobic ammonium oxidation microorganisms serving as the single nitrogen source comprises the following steps of:
(1) pretreatment of inoculated sludge: anaerobic ammonia oxidation sludge granular sludge is used as an inoculum, the grain diameter of the granular sludge is 1.0-3.0mm, after 15d hunger pretreatment, ultrasonic treatment is carried out for 1h to promote particle disintegration and flocculation, 400 ml of flocculent sludge (MLSS about 3000-3500 mg/L) is inoculated and placed in a cathode reactor of a double-cavity electrolytic cell with the volume of 1L for acclimatization and culture, and the voltage range of a direct current stabilized voltage power supply of the double-cavity electrolytic cell is between 0.8 and 1.2V.
(2) Controlling the working condition of domestication and culture of the electroactive anaerobic ammonium oxidation microorganisms: controlling the concentration of flocculent sludge in the cathode reactor at 2500-4000mg/L, adding 0.5% penicillin to inhibit the growth of heterotrophic bacteria, introducing Ar and CO 2 Creating an anaerobic environment, ar and CO 2 Is 95%:5% initial NH 4 + With NO 2 - The concentration of the raw materials is 100mg-N/L and 120mg-N/L respectively, the operation is carried out continuously, the Hydraulic Retention Time (HRT) is 12h, and NaHCO is supplemented every 2d 3 And the trace elements are supplied for the growth of the anaerobic ammonium oxidation microorganisms, the load is increased by 25 percent when every 10 days is taken as a period, and the stirring paddle is stirred for 10min every 60min to ensure the uniform mass transfer in the system.
As shown in FIG. 1, water NH is fed when the reactor is operated to 40d 4 + With NO 2 - The concentration of the nitrogen reaches 300mg-N/L and 360mg-N/L respectively, and the nitrogen removal rate is stabilized to be more than 75 percent and then the operation is stably carried out for 20 days. From 60d, NH is gradually reduced by 25 percent under the premise of keeping the mass conservation of the substrate nitrogen 4 + The ratio is 0, the total nitrogen removal rate is still over 75%, and the phenomenon shows that the reduction of NO by cathode electrons is established 2 -generating N 2 A reaction path for domesticating the anaerobic ammonium oxidation microorganism with electric activity.
(3) Nitrate (NO) reduction by activated cathode electron-donating electroactive anammox microorganisms 3 - ) Accumulation of Nitrite (NO) 2 -) control of the operating conditions of the route: in a cathode reactor for successfully domesticating electroactive microorganisms, the substrate composition is changed with NO 3 As nitrogen source, 1% of ferric disodium EDTA is added simultaneously to facilitate the excitation of the DNRA pathway.
As shown in fig. 2, initial water intake NO 3 - The concentration is 120mg-N/L, the operation is continuous, the Hydraulic Retention Time (HRT) is 12h, and NaHCO is supplemented every 2d 3 And supply of trace elements to anammox microorganismsGrowing the plant, and increasing the load by 25 percent in every 10 days as a period till the NO of the inlet water of the system from the 50 th day 3 - The concentration reaches 360mg-N/L, NO 3 - The removal rate is stabilized to be more than 75 percent, and the phenomenon shows that the electroactive anaerobic ammonia oxidation microorganisms reduce Nitrate (NO) 3 -) accumulation of Nitrite (NO) 2 - ) Is excited to successfully reduce NO with cathode electrons 2 - Generating N 2 Thereby realizing the NO of the electroactive anaerobic ammonium oxidation microorganism in the water body 3 - Effective removal of, i.e. with NO 3 - The electrically active anaerobic ammonium oxidation microorganism which is a single nitrogen source is successfully domesticated, and the specific anaerobic ammonium oxidation activity can reach 0.3 g-N/(g) -1 -VSSd -1 ) As described above.
The experimental process shows that the anaerobic ammonia oxidizing microorganisms with electric activity can realize extracellular electron transfer through an external electric field, and the metabolic pathway of reducing dissimilatory nitrate of the anaerobic ammonia oxidizing microorganisms into ammonia (DNRA) can be excited to reduce NO under the condition of sufficient electron donor 3 To accumulate NO 2 To thereby realize NO in the water body 3 - And (4) removing. Thus, acclimatization of the culture with NO 3 - Is an electroactive anaerobic ammonium oxidation microorganism with a single nitrogen source, and can effectively realize NO in water 3 - The removal of the carbon source has positive significance for solving the problem of eutrophication of the water body, and particularly has better treatment effect for treating sewage with low carbon-nitrogen ratio without adding a carbon source.
The invention domesticates anaerobic ammonia oxidizing microorganisms with electric activity, and reduces NO by electrons provided by an electric field cathode 3 - Accumulation of NO 2 - And replacing NH by cathodic electrons 4 + Position of electron donor to realize single NO 3 - Anaerobic ammoxidation reaction in nitrogen source mode to reach NO in water 3 - The autotrophic removal of (1).
While one embodiment of the present invention has been described in detail, the description is only a preferred embodiment of the present invention and should not be taken as limiting the scope of the invention. All equivalent changes and modifications made within the scope of the present invention shall fall within the scope of the present invention.
Claims (4)
1. With NO 3 - The domestication culture method of the electroactive anaerobic ammonium oxidation microorganisms which are single nitrogen sources is characterized by comprising the following steps of: the method comprises the following steps:
(1) pretreatment of inoculated sludge: anaerobic ammonium oxidation sludge granular sludge is used as an inoculum, after starvation pretreatment, particles are ultrasonically promoted to disintegrate and flocculate, and flocculent sludge is inoculated into a cathode reaction chamber of the double-cavity electrolytic cell after sedimentation and concentration for acclimatization and culture;
(2) domestication culture of the electroactive anaerobic ammonium oxidation microorganisms: controlling the concentration of flocculent sludge in the cathode reactor at 2500-4000mg/L, adding 0.5% penicillin to inhibit the growth of heterotrophic bacteria, introducing Ar and CO 2 Creating an anaerobic environment, initial NH 4 + With NO 2 - The concentration of the water is 100mg-N/L and 120mg-N/L respectively, the operation is carried out continuously, the hydraulic retention time is 12h, and NaHCO is supplemented every 2d 3 And the microelements are supplied for the growth of the anaerobic ammonium oxidation microorganisms, the load is increased by 25 percent when every 10 days is taken as a period, and the stirring paddle is used for stirring to ensure the uniform mass transfer in the system;
NH to be fed into the cathode reactor 4 + With NO 2 - The concentration of the nitrogen reaches 300mg-N/L and 360mg-N/L respectively, when the total nitrogen removal rate is stabilized to be more than 75 percent, NH is gradually reduced by 25 percent on the premise of keeping the mass conservation of the substrate nitrogen 4 + Up to 0, thereby establishing the cathodic electron reduction of NO 2 - Generating N 2 The generation of a reaction channel, domesticating anaerobic ammonium oxidation microorganisms with electric activity;
(3) nitrate (NO) reduction by activated cathode electron-donating electroactive anammox microorganisms 3 - ) Accumulation of Nitrite (NO) 2 - ) The method comprises the following steps: in said cathodic reactor, which successfully acclimatizes the electroactive microorganisms, the substrate composition is changed with NO 3 - As a nitrogen source, 1% ofFerric disodium EDTA to facilitate the priming of the DNRA pathway;
initial NO 3 - The concentration is 120mg-N/L, the operation is continuous, the Hydraulic Retention Time (HRT) is 12h, and NaHCO is supplemented every 2d 3 And the trace elements are supplied for the growth of the anaerobic ammonium oxidation microorganisms, the load is increased by 25 percent as a period of every 10 days until the water inlet NO of the system 3 - The concentration reaches 360mg-N/L, NO 3 - When the removal rate is stabilized to be more than 75 percent, the electro-active anaerobic ammonium oxidation microorganisms are shown to reduce Nitrate (NO) 3 - ) Accumulation of Nitrite (NO) 2 - ) Is excited and further reduces NO using cathode electrons 2 - Generating N 2 Thereby realizing the NO of the electroactive anaerobic ammonium oxidation microorganism in the water body 3 - Effective removal of (i.e. with NO) 3 - The electrically active anaerobic ammonium oxidation microorganism which is a single nitrogen source is successfully domesticated, and the specific anaerobic ammonium oxidation activity can reach 0.3 g-N/(g) -1 -VSS·d -1 ) The above.
2. The method of claim 1 with NO 3 - The domestication culture method of the electroactive anaerobic ammonium oxidation microorganisms which are single nitrogen sources is characterized by comprising the following steps: in the step (1), the particle size of the granular sludge is 1.0-3.0mm, and after starvation pretreatment is carried out for 5-15 days, the particles are subjected to ultrasonic treatment for 1 hour to promote disintegration and flocculation;
in step (2), ar reacts with CO 2 Is 95%:5 percent, and the stirring paddle stirs for 10min every 60min to ensure the uniform mass transfer in the system.
3. The method of claim 1 or 2 with NO 3 - The domestication culture method of the electroactive anaerobic ammonium oxidation microorganisms which are single nitrogen sources is characterized by comprising the following steps: and (3) adopting a double-cavity electrolytic cell as a reaction device, controlling the voltage range of 0.8-1.2V by a direct-current stabilized voltage supply, and performing domestication and enrichment on the electroactive anaerobic ammonium oxidation microorganisms in a cathode reaction cell.
4. Use of the microorganism obtained in any one of claims 1 to 3 for the treatment of low carbon to nitrogen ratio sewage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210783293.XA CN115520962B (en) | 2022-07-05 | 2022-07-05 | With NO3-Method for domesticating and culturing electroactive anaerobic ammonia oxidizing microorganism serving as single nitrogen source |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210783293.XA CN115520962B (en) | 2022-07-05 | 2022-07-05 | With NO3-Method for domesticating and culturing electroactive anaerobic ammonia oxidizing microorganism serving as single nitrogen source |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115520962A true CN115520962A (en) | 2022-12-27 |
| CN115520962B CN115520962B (en) | 2024-05-03 |
Family
ID=84695803
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210783293.XA Active CN115520962B (en) | 2022-07-05 | 2022-07-05 | With NO3-Method for domesticating and culturing electroactive anaerobic ammonia oxidizing microorganism serving as single nitrogen source |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115520962B (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006246847A (en) * | 2005-03-14 | 2006-09-21 | Hitachi Plant Technologies Ltd | Method and device for culturing anaerobic ammonia-oxidizing bacterium |
| CN104386814A (en) * | 2014-12-02 | 2015-03-04 | 河北工业大学 | Method for intensifying starting performance of anaerobic ammonia oxidation process by using ultrasonic wave |
| CN105174448A (en) * | 2015-10-13 | 2015-12-23 | 沈阳建筑大学 | Anaerobic ammonia oxidation and denitrification coupling device and controlled operation method thereof |
| CN107555592A (en) * | 2017-09-20 | 2018-01-09 | 苏州科技大学 | Anaerobic ammonium oxidation granular sludge cultural method and culture apparatus |
| CN110723812A (en) * | 2019-09-17 | 2020-01-24 | 北京工业大学 | Method for improving pH impact resistance of anaerobic ammonium oxidation particles |
| CN111573821A (en) * | 2020-05-22 | 2020-08-25 | 盐城工学院 | Electrode denitrification system based on autotrophic short-cut denitrification-anaerobic ammonia oxidation module |
| CN112939213A (en) * | 2021-04-01 | 2021-06-11 | 北京工业大学 | Rapid CANON granular sludge starting method based on hydraulic screening intermittent starvation |
| CN113683188A (en) * | 2021-09-13 | 2021-11-23 | 江苏大学 | Method and device for electrochemically domesticating anaerobic ammonium oxidation bacteria |
-
2022
- 2022-07-05 CN CN202210783293.XA patent/CN115520962B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006246847A (en) * | 2005-03-14 | 2006-09-21 | Hitachi Plant Technologies Ltd | Method and device for culturing anaerobic ammonia-oxidizing bacterium |
| CN104386814A (en) * | 2014-12-02 | 2015-03-04 | 河北工业大学 | Method for intensifying starting performance of anaerobic ammonia oxidation process by using ultrasonic wave |
| CN105174448A (en) * | 2015-10-13 | 2015-12-23 | 沈阳建筑大学 | Anaerobic ammonia oxidation and denitrification coupling device and controlled operation method thereof |
| CN107555592A (en) * | 2017-09-20 | 2018-01-09 | 苏州科技大学 | Anaerobic ammonium oxidation granular sludge cultural method and culture apparatus |
| CN110723812A (en) * | 2019-09-17 | 2020-01-24 | 北京工业大学 | Method for improving pH impact resistance of anaerobic ammonium oxidation particles |
| CN111573821A (en) * | 2020-05-22 | 2020-08-25 | 盐城工学院 | Electrode denitrification system based on autotrophic short-cut denitrification-anaerobic ammonia oxidation module |
| CN112939213A (en) * | 2021-04-01 | 2021-06-11 | 北京工业大学 | Rapid CANON granular sludge starting method based on hydraulic screening intermittent starvation |
| CN113683188A (en) * | 2021-09-13 | 2021-11-23 | 江苏大学 | Method and device for electrochemically domesticating anaerobic ammonium oxidation bacteria |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115520962B (en) | 2024-05-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ma et al. | Electricity generation from swine wastewater in microbial fuel cell: Hydraulic reaction time effect | |
| Nguyen et al. | Insights on microbial fuel cells for sustainable biological nitrogen removal from wastewater: A review | |
| CN107032479B (en) | Method for strengthening treatment of high-concentration ciprofloxacin wastewater by bioelectrochemical anaerobic/microelectrolysis blending coupling | |
| CN101844857B (en) | New process for micro electric field reinforced low-carbon nitrogen removal | |
| Zhu et al. | Electrostimulation enhanced ammonium removal during Fe (III) reduction coupled with anaerobic ammonium oxidation (Feammox) process | |
| CN110683643B (en) | Enrichment method of anaerobic ammonium oxidation bacteria | |
| Wang et al. | A review of partial nitrification in biological nitrogen removal processes: from development to application | |
| Zhong et al. | Power recovery coupled with sulfide and nitrate removal in separate chambers using a microbial fuel cell | |
| CN109292959B (en) | Method for enhancing cathode denitrification of wastewater with low CN ratio | |
| Qiao et al. | Reinforced nitrite supplement by cathode nitrate reduction with a bio-electrochemical system coupled anammox reactor | |
| CN113277613B (en) | Electrode regulation-based integrated biological autotrophic nitrogen removal system, method and application | |
| CN103086508A (en) | Microbial fuel cell wastewater treatment system for improving nitrogen removal effect | |
| CN102249424A (en) | Proton exchange membrane-free microbiological fuel cell sewage treatment system and application method thereof | |
| CN112607864A (en) | Electrochemical performance-enhanced bacteria-algae membrane aeration biomembrane reactor system and application thereof | |
| Zhang et al. | A critical review of improving mainstream anammox systems: Based on macroscopic process regulation and microscopic enhancement mechanisms | |
| CN111573834A (en) | Reactor based on short-cut denitrification electrode is in coordination with anaerobic ammonia oxidation denitrogenation | |
| Guo et al. | Simultaneous removal of organics, sulfide and ammonium coupled with electricity generation in a loop microbial fuel cell system | |
| Zhu et al. | Anticipating on potential electron acceptors for Anammox | |
| CN114149086B (en) | Enrichment method of anaerobic ammonium oxidation bacteria, ammonia nitrogen removal method and device | |
| CN115520962A (en) | With NO 3- Domestication culture method of electroactive anaerobic ammonium oxidation microorganisms serving as single nitrogen source | |
| CN113248016B (en) | Method for strengthening one-stage completely autotrophic nitrogen removal process by embedded cathode dynamic membrane | |
| CN105110561A (en) | Method for treating high ammonia-nitrogen wastewater under low dissolved oxygen condition | |
| CN112759065B (en) | Method for quickly starting anaerobic ammonia oxidation reactor and recovering activity of anaerobic ammonia oxidation bacteria | |
| CN113461170B (en) | Method and equipment for rapidly culturing autotrophic denitrification cable bacteria and application | |
| CN109160603B (en) | Denitrifying bacterium enrichment method for development of oilfield reinjection water anticorrosive bactericide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |