CN114149086B - Enrichment method of anaerobic ammonium oxidation bacteria, ammonia nitrogen removal method and device - Google Patents

Enrichment method of anaerobic ammonium oxidation bacteria, ammonia nitrogen removal method and device Download PDF

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CN114149086B
CN114149086B CN202111431261.5A CN202111431261A CN114149086B CN 114149086 B CN114149086 B CN 114149086B CN 202111431261 A CN202111431261 A CN 202111431261A CN 114149086 B CN114149086 B CN 114149086B
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CN114149086A (en
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唐溪
吴筱筱
赵江伟
唐崇俭
姚福兵
王云燕
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Central South University
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C—CHEMISTRY; METALLURGY
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Abstract

The invention discloses an enrichment method of electroactive anaerobic ammonium oxidation bacteria, an ammonia nitrogen removal method and an ammonia nitrogen removal device. Specifically, under the conditions that ammonia nitrogen exists in a substrate and nitrite does not exist, anaerobic ammonia oxidation sludge is added inside, voltage is applied, an anaerobic ammonia oxidation microorganism electrochemical reaction system is operated, and the electrically active anaerobic ammonia oxidation bacteria are obtained through enrichment. The system is continuously operated to remove ammonia nitrogen. The method can efficiently enrich the electroactive anaerobic ammonium oxygen bacteria and is used for the efficient operation of an anaerobic ammonium oxidation microorganism reaction system. The problem that when the anaerobic ammonia oxidation process is applied to actual wastewater treatment, nitrite nitrogen is required to be used as an electron acceptor, namely, a short-range nitrification process or a short-range denitrification process must be connected to the front end of the anaerobic ammonia oxidation process, other nitrogen in a water body is used for converting nitrite, and then the anaerobic ammonia oxidation process is combined to remove ammonia nitrogen in wastewater; the problem that the deep denitrification can not be directly carried out in one step in the water body without nitrite is solved.

Description

Enrichment method of anaerobic ammonium oxidation bacteria, ammonia nitrogen removal method and device
Technical Field
The invention relates to the technical field of anaerobic ammonia oxidation, in particular to an enrichment method of electroactive anaerobic ammonia oxidizing bacteria, an ammonia nitrogen removal method and an ammonia nitrogen removal device.
Background
In the field of biological denitrification of wastewater at present, functional floras of an electrochemical denitrification system based on microorganisms are mostly ammonia oxidizing bacteria, nitrite oxidizing bacteria, denitrifying bacteria and the like regardless of external electric energy, and the electrochemical denitrification system is characterized in that the bacteria of a plurality of functional floras are required to cooperate with one another to complete the biological denitrification process in the wastewater, the reaction route is long, or aeration is required, or a carbon source is required to be added externally and the like, so that the removal of ammonia nitrogen cannot be realized economically, energy is saved, and the ammonia nitrogen can be removed in one step.
The application develops a method for enriching high-concentration electroactive anaerobic ammonium oxygen bacteria, and the method can efficiently enrich the electroactive anaerobic ammonium oxygen bacteria and provide a guarantee foundation for efficient operation of an anaerobic ammonia oxidation microbial reaction system. The electro-active anaerobic ammonium-oxygen bacteria enriched by the method can successfully operate the anaerobic ammonia oxidation microorganism electrochemical reactor under the condition that the substrate only contains ammonia nitrogen and does not contain nitrite, thereby realizing the removal of the ammonia nitrogen in one step.
Disclosure of Invention
The invention aims to provide a method for enriching high-concentration electroactive anaerobic ammonium oxygen bacteria. The method can efficiently enrich the electroactive anaerobic ammonium oxygen bacteria and is used for the efficient operation of an anaerobic ammonium oxidation microorganism reaction system.
An enrichment method of electroactive anaerobic ammonium oxidation bacteria comprises the following steps: under the conditions that the substrate only contains ammonia nitrogen and does not contain nitrite, adding the anaerobic ammonia oxidation sludge, applying voltage, operating an anaerobic ammonia oxidation microorganism electrochemical reaction system, and enriching to obtain the electroactive anaerobic ammonia oxidation bacteria.
The enrichment method of the electroactive anaerobic ammonium oxidation bacteria comprises the following steps: the enrichment to obtain the electroactive anaerobic ammonium oxidation bacteria comprises the following steps: anammox bacteria.
The enrichment method of the electroactive anaerobic ammonium oxidation bacteria comprises the following steps: the anaerobic ammonia oxidation microorganism electrochemical reaction system is characterized in that an electrode, preferably three electrodes, is added into the anaerobic ammonia oxidation microorganism electrochemical reaction system.
The enrichment method of the electroactive anaerobic ammonium oxidation bacteria comprises the following steps: the wastewater treated in the anaerobic ammonia oxidation microorganism electrochemical reaction system is aerated to remove dissolved oxygen in the wastewater, and preferably: the dissolved oxygen was kept below 0.05mg/L and the pH was adjusted to 6.8-7.2.
The enrichment method of the electroactive anaerobic ammonium oxidation bacteria comprises the following steps: the anaerobic ammoxidation microorganism electrochemical reaction system is placed in an anaerobic environment at the temperature of 30 plus or minus 5 ℃.
The enrichment method of the electroactive anaerobic ammonium oxidation bacteria comprises the following steps: the anaerobic ammonia oxidation microorganism electrochemical reaction system is operated intermittently in batches, HRT is 24-72h, and 1/2-1/4 volume of solution is replaced in 8-16 h.
The enrichment method of the electroactive anaerobic ammonium oxidation bacteria comprises the following steps: the applied voltage does not exceed 0.6v.
The reaction system of the invention maintains strict anaerobic environment in the whole period.
Anaerobic ammonia oxidation is anaerobic bacteria, other flora can become dominant bacteria if dissolved oxygen exists, and strict anaerobism can inhibit other flora. In addition, in the electrochemical reaction, oxygen can participate in the oxidation-reduction reaction in the presence of oxygen, so that the reaction is more complicated, the denitrification performance is reduced, and the coulomb efficiency is reduced.
Furthermore, the long-term domestication of the electroactive anaerobic ammonium oxidation bacteria adopts artificially prepared simulated wastewater, and the main components are as follows: 100mg L -1 NH 4 + ，0.3mg L -1 MgSO 4 ·7H 2 O，1.25mg L -1 KHCO 3 . Wherein 1.25mg L is also added -1 Trace element solution I, 1.25mg L -1 Trace element solution II, 1mg L -1 And (3) a trace element solution III. The microelement solution I comprises 5g L -1 EDTA and 9.14g L -1 FeSO 4 ·7H 2 O; the microelement II comprises 15gL -1 Na 2 EDTA、0.43gL -1 ZnSO 4 ·7H 2 O、0.283gL -1 CoSO 4 ·7H 2 O、0.845gL -1 MnSO 4 ·H 2 O、0.25gL -1 CuSO 4 ·5H 2 O、0.19gL -1 NiSO 4 ·6H 2 0、0.014gL -1 H 3 BO 4 (ii) a The microelement solution III comprises 0.0056gL -1 CaCl 2 ·2H 2 O and 0.01gL -1 NaH 2 PO 4 ·2H 2 And O. All simulated wastewater uses N 2 /CO 2 (95/5%) was aerated to remove dissolved oxygen in the wastewater. Contains no organic carbon and nitrite.
The enriched bacterial community assay of the invention was obtained by 16S rRNA technology.
The second purpose of the invention is to provide an ammonia nitrogen removal method, which is to continue to operate after the anaerobic ammonia oxidation microorganism electrochemical reaction system in the enrichment method is directly utilized to complete enrichment.
In the nitrite-free microbial electrochemical system, under a proper voltage, the high-enriched electroactive anaerobic ammonium oxidation bacteria can directly perform NH on the anode 4 + The ammonia nitrogen is removed in one step by oxidation (nitrogen is generated at the end of the anode), and the hydrogen generated at the cathode can be collected by a gas recovery system.
The third purpose of the invention is to provide the enrichment method or the device matched with the ammonia nitrogen removal method, wherein the device comprises the following components: comprises a water inlet system, an anaerobic ammonia oxidation microbial electrochemical reactor and a water outlet system;
the water inlet system injects the aerated wastewater into the anaerobic ammonia oxidation microbial electrochemical reactor for treatment;
the anaerobic ammonia oxidation microbial electrochemical reactor is internally provided with three electrodes comprising a reference electrode, an anode and a cathode, wherein the three electrodes are positioned in the wastewater in the treatment process; the external part is provided with a constant potential rectifier connected with the internal three electrodes, and the bottom part is provided with a magnetic stirring system;
the water outlet system discharges the treated wastewater from the anaerobic ammonia oxidation microbial electrochemical reactor.
Further, the three electrodes adopt graphite felts as working electrodes and auxiliary electrodes, and silver chloride electrodes as reference electrodes; the area of the anode graphite felt is preferably 4-16cm 2 The area of the cathode graphite felt is 36-64cm 2 The thickness is 0.5cm +/-0.05 cm.
Further, the water inlet system comprises an anaerobic feed supplement container, and a first aeration bottle, an air pressure stabilizing bag and a water inlet peristaltic pump which are respectively connected to the anaerobic feed supplement container; the anaerobic feed supplement container is filled with wastewater to be treated, the first aeration bottle is connected to the wastewater and aerates the wastewater, the air pressure stabilizing bag is connected to the upper part of the liquid level of the wastewater to adjust air pressure, and the water inlet peristaltic pump is connected to the wastewater and is controlled by the water inlet controller to convey the wastewater to the anaerobic ammonia oxidation microorganism electrochemical reactor.
Furthermore, a second aeration bottle and a double-valve side hydrogen collecting device are arranged outside the anaerobic ammonia oxidation microorganism electrochemical reactor, the second aeration bottle is connected to the wastewater to aerate the wastewater, and the double-valve side hydrogen collecting device is connected to the upper part of the wastewater liquid level to collect gas generated by wastewater treatment so as to adjust the gas pressure.
Furthermore, the water outlet system comprises a water outlet peristaltic pump and a water storage barrel, wherein the water outlet peristaltic pump is connected to the wastewater in the anaerobic ammonia oxidation microbial electrochemical reactor and is controlled by the water outlet controller to convey the wastewater to the water storage barrel.
The implementation process of the invention is as follows:
(1) Screening and enriching microorganisms: inoculating anaerobic ammonia oxidation sludge according to about 5 percent of the working volume of the reactor, and simultaneously injecting artificial simulated ammonia nitrogen wastewater without nitrite into the reactor. Then a circuit is communicated, a constant potential rectifier is opened, a small amount of voltage is applied to the two poles of the reactor, the proper temperature is controlled, a magnetic stirrer plays a certain stirring role in the reaction, the microorganism is screened and enriched, and an electrochemical workstation is used for recording current;
(2) As the screened microorganisms are enriched on the surface of the electrode, the current rises (relative to the background current) and the ammonia nitrogen concentration of the reactor begins to decrease, a CV diagram has obvious oxidation-reduction peaks and nitrogen and hydrogen are generated. And TEM and 16sRNA show that the microbial flora contains high-concentration anaerobic ammonia oxidation flora, so that the screening and enrichment of the electroactive anaerobic ammonia oxidation bacteria are completed. Then adding an organic substance Allyl Thiourea (ATU) for inhibiting other flora into the reactor, observing the ammonia nitrogen removal rate, and finding no obvious change.
(3) Electrochemical assisted biological denitrification: after the screening and enrichment of the microorganisms are completed, a sequencing batch operation mode is adopted, other conditions are kept unchanged, and the long-term denitrification process of the electrochemical auxiliary electroactive anaerobic ammonium oxidation bacteria is started. Domesticating and culturing for a long time to achieve higher denitrification effect; discharging 1/3 mixed liquor in the reactor in a sequencing batch mode at one time, re-injecting ammonia nitrogen wastewater, keeping other conditions unchanged, and starting an electrochemical auxiliary biological denitrification process.
The invention has the beneficial effects that:
(1) A method for enriching high-concentration electroactive anaerobic ammonium oxygen bacteria is developed, the method can efficiently enrich the electroactive anaerobic ammonium oxygen bacteria, and a guarantee foundation is provided for efficient operation of an anaerobic ammonia oxidation microbial reaction system.
(2) Aiming at low ammonia nitrogen wastewater, the anaerobic ammonium oxidation reaction is completed without additionally adding nitrite. Can directly react NH 4 + By oxidation to N 2 And by-product NO is avoided 3 - The pressure of the subsequent treatment process is reduced.
(3) The problem that when the anaerobic ammonia oxidation process is applied to actual wastewater treatment, nitrite nitrogen is required to be used as an electron acceptor, namely, a short-range nitrification process or a short-range denitrification process must be connected to the front end of the anaerobic ammonia oxidation process, other nitrogen in a water body is used for converting nitrite, and then the anaerobic ammonia oxidation process is combined to remove ammonia nitrogen in wastewater; the problem that the deep denitrification can not be directly carried out in one step in the water body without nitrite is solved.
(4) The method solves the problem that functional floras of a microorganism-based electrochemical denitrification system are mostly ammonia oxidizing bacteria, nitrite oxidizing bacteria, denitrifying bacteria and the like regardless of external electric energy in the field of biological denitrification of wastewater at present, and has the common characteristic that the biological denitrification process in the wastewater can be completed only by the mutual cooperation of the bacteria of a plurality of functional floras, the reaction route is long, or aeration is needed, or a carbon source is needed to be added externally and the like, so that the removal of ammonia nitrogen cannot be realized economically and in an energy-saving manner in one step. "is used in the above-mentioned patent publication.
Drawings
FIG. 1 is a diagram of the apparatus of the present invention;
wherein 1-potentiostat; 2-a double-valve side hydrogen collecting device; 3-a water inlet; 4-a reference electrode; 5-an anode electrode; 6-a cathode electrode; 7-water intake controller 1; 8-a water inlet peristaltic pump; 9-a first aeration cylinder; 10-air pressure stabilizing bag; 11-anaerobic feed supplement container; 12-a stirrer; 13-a magnetic stirrer; 14-anammox sludge; 15-a second aeration cylinder; 16-water outlet controller 2; 17-a water outlet peristaltic pump; 18-a water storage barrel; 19-water outlet; 20-anaerobic ammonia oxidation microbial electrochemical reactor.
FIG. 2 is a change in microbial community structure (genus level);
b1 is an unacclimated stage, A1 is acclimated for two months, and C1 is ATU-added.
FIG. 3 is a diagram showing the removal of ammonia nitrogen, nitrite and nitrate in the acclimation process of the reactor.
FIG. 4 is a diagram of CV at the post-reactor acclimation stage.
FIG. 5 is a diagram showing the effect of ATU on ammonia nitrogen removal in an anammox-microbial electrochemical system.
FIG. 6 is a diagram of ammonia nitrogen removal in an anammox-microbial electrochemical system after acclimation.
FIG. 7 is a diagram showing the adhesion of anammox bacteria to the electrode surface.
FIG. 8 is a CV diagram of anammox bacteria before and after acclimation.
Detailed Description
The following examples are intended to further illustrate the invention without limiting it.
Example 1
The device comprises a water inlet system, an anaerobic ammonia oxidation microorganism electrochemical reactor and a water outlet system.
The water inlet system injects the aerated wastewater into the anaerobic ammonia oxidation microbial electrochemical reactor for treatment; the anaerobic ammonia oxidation microbial electrochemical reactor is internally provided with three electrodes comprising a reference electrode, an anode and a cathode, wherein the three electrodes are positioned in the wastewater in the treatment process; a potentiostat (with the function of a data collector and used for collecting current) connected with the three internal electrodes is arranged outside the magnetic stirring device, and a magnetic stirring system is arranged at the bottom of the magnetic stirring device; the effluent system discharges treated wastewater from the anaerobic ammonia oxidation microbial electrochemical reactor. The three electrodes adopt graphite felts as working electrodes and auxiliary electrodes, and silver chloride electrodes as reference electrodes; the area of the anode graphite felt is 4-16cm 2 The area of the cathode graphite felt is 36-64cm 2 All thickness is 0.5cm + -0.05 cm. The water inlet system comprises an anaerobic feed supplement container, a first aeration bottle (keeping the interior of the water inlet feed supplement bottle strictly anaerobic), an air pressure stabilizing bag (keeping the interior air pressure stable) and a water inlet peristaltic pump, wherein the first aeration bottle, the air pressure stabilizing bag and the water inlet peristaltic pump are respectively connected to the anaerobic feed supplement container; the anaerobic feed supplement container is filled with wastewater to be treated, the first aeration bottle is connected to the wastewater and aerates the wastewater, the air pressure stabilizing bag is connected to the upper part of the liquid level of the wastewater to adjust air pressure, and the water inlet peristaltic pump is connected to the wastewater and is controlled by the water inlet controller to convey the wastewater to the anaerobic ammonia oxidation microorganism electrochemical reactor. The anaerobic ammonia oxidation microorganism electrochemical reactor is also provided with a second aeration bottle (for internal aeration of the reactor and maintaining anaerobic state) and a double-valve side hydrogen collecting device (for maintaining stable internal air pressure and collecting hydrogen and nitrogen), the second aeration bottle is connected to the wastewater to aerate the wastewater, and the double-valve side hydrogen collecting device is connected to the upper part of the wastewater liquid level to collect gas generated by wastewater treatment so as to adjust the air pressure. The water outlet system comprises a water outlet peristaltic pump and a water storage barrel, wherein the water outlet peristaltic pump is connected to the wastewater of the anaerobic ammonia oxidation microbial electrochemical reactor and is controlled by the water outlet controller to convey the wastewater into the water storage barrel. The device is constructed as shown in figure 1.
Example 2
The anaerobic ammonia oxidation microbial electrochemical reactor is a 500ml (phi 80 x 110mm) cylindrical acrylic container; the ammonia nitrogen artificial simulation wastewater (injected with 500 ml) mainly comprises the following components: 100mg L -1 NH 4 + ，0.3mg L -1 MgSO 4 ·7H 2 O，1.25mg L -1 KHCO 3 . Wherein 1.25mg L is also added -1 Trace element solution I, 1.25mg L -1 Trace element solution II, 1mg L -1 And (3) a trace element solution III. The microelement solution I comprises 5g L -1 EDTA and 9.14g L -1 FeSO 4 ·7H 2 O; the microelement II comprises 15gL -1 Na 2 EDTA、0.43gL -1 ZnSO 4 ·7H 2 O、0.283gL -1 CoSO 4 ·7H 2 O、0.845gL -1 MnSO 4 ·H 2 O、0.25gL -1 CuSO 4 ·5H 2 O、0.19gL -1 NiSO 4 ·6H 2 0、0.014gL -1 H 3 BO 4 (ii) a The microelement solution III comprises 0.0056gL -1 CaCl 2 ·2H 2 O and 0.01gL -1 NaH 2 PO 4 ·2H 2 And O. All simulated wastewater uses N 2 /CO 2 (95/5%) was aerated to remove dissolved oxygen in the wastewater so that the dissolved oxygen amount was kept below 0.05mg/L and the pH was adjusted to around 7. Contains no organic carbon and nitrite.
Inoculating anaerobic ammonium oxidation sludge according to about 5 percent of the working volume of the reactor. The first three of the phyla of the relative abundance of the colonies are the phylum Nostoc, the phylum Proteus and the phylum Zygomycota viridis. Two species of anammox bacteria, candidatus Jettenia and Candidatus Brocadia, were detected at the genus level.
Furthermore, the reactor is placed in a greenhouse at 30 +/-5 ℃ in a full period, the strict anaerobic environment is kept in the full period of the reactor, and the rotating speed is controlled to be about 100 r/min. The long-term voltage supply is provided by DJS-292D potentiostat of Shanghai Xin Rui apparatus company, the area of the anode graphite felt is 8cm 2 The area of the cathode graphite felt is 36cm 2 And applying voltage of 0v-0.6v to screen and enrich the microorganisms. The voltage intensity is controlled to be 0.4v, 0.2v and 0v by a constant potential rectifier. Gradually domesticating and enriching the electro-active anaerobic ammonium oxidation bacteria. Acclimatizing and enriching at 0.4v for 25 days at HRT of 36h for NH 4 + The removal of (A) was maintained substantially at about 30-50%, followed by 0.2v acclimatization and enrichment for about 15 days, and finally applying 0v, NH at 36h HRT 4 + Is substantially 40% or more. After two months of acclimation, the enrichment degree of the anaerobic ammonium oxidation bacteria can account for more than 70 percent.
HRT (hydraulic retention time) refers to the average retention time of the sewage to be treated in the reactor. Removal rate of ammonium ions: and subtracting the ammonia nitrogen concentration in the outlet water from the ammonia nitrogen concentration in the inlet water, and dividing by the ammonia nitrogen concentration in the inlet water.
Then adding organic Allyl Thiourea (ATU) for inhibiting other flora reactions into the reactor, and observing that the ammonia nitrogen removal rate has no obvious change. This represents the successful domestication and enrichment of the electroactive anammox bacteria. The results are shown in FIG. 2. FIG. 3 shows the denitrification at 0.4,0.2, 0v.
The acclimation stage and the later operation stage adopt HRT 36h and 12h for replacing 1/3 volume of solution.
Example 3
The system constitution and the process of the embodiment 3 and the embodiment 2 are the same. The difference is that the constant voltage data is supplied by Chenghua electrochemical workstation; and c, carrying out cv curve scanning on the reactors subjected to acclimatization under different voltages to see whether reversible redox reactions exist or not. From the CV diagram (FIG. 4), a distinct redox peak is observed, and the redox peak is supposed to be a redox peak produced by cytochrome c.
Example 4
The system constitution and the process of the embodiment 4 and the embodiment 2 are the same. The difference lies in that after domestication is carried out for 2 months, allylthiourea (ATU) capable of inhibiting AOB bacteria is added into a reactor, and whether the anammox bacteria also has the function of removing ammonia nitrogen under the action of voltage is explored after interference of other flora is eliminated. The results are shown in FIG. 5. Under the action of ATU, ammonia nitrogen is still removed in the system, and the effect of anaerobic ammonium oxidation bacteria can be determined. NH is shown in FIG. 5 4 + And NO 3 - A trend of concentration over time; Δ NH 4 + Is a difference representing two adjacent values.
Example 5
The system constitution and the process of example 5 are the same as those of example 1. The difference lies in that in the post-acclimation period, the deep denitrification performance is explored under the condition of low pressure when the voltage is applied to 0v. FIG. 6 shows that enriched electroactive anammox bacteria can directly release NH in the absence of nitrous acid 4 + Direct conversion to N 2 Conversion of a minor portion to NO 3 - 。
Example 6
According to the invention, by comparing the enrichment degree of the flocculent sludge and the granular sludge in the reactor, the electric activity strength and the denitrification performance of the anaerobic ammonia oxidizing bacteria in the later domestication period, the flocculent sludge is found to be more easily attached to the surface of the electrode, and the granular sludge is hardly attached to the electrode plate. The contact area of the flocculent sludge and the electrode plate is larger, the electron transfer is more active, and the denitrification performance (improved by 30%) and the growth state are better.
Example 7
According to the invention, by comparing the enrichment degree of the graphite rod and the graphite felt as the electrodes in the reactor, the electric activity strength and the denitrification performance of the anaerobic ammonium oxidation bacteria in the later domestication period, the graphite felt as the electrode plate has larger contact area, more active electron transfer, better denitrification performance (improved by 50%) and better sludge growth state, and the figure 7 shows.
Example 8
In order to determine the influence of the anode/cathode as the working electrode, the system of the invention also carries out experimental comparison of-0.1 v and +0.1v, and finds that when positive pressure is applied, namely the working electrode is the anode, the ammonia nitrogen removal rate is more stable, the overall removal effect is better, nitrate is less enriched, and the removal of ammonia nitrogen is facilitated.
Example 9
The system of the present invention also makes a distinction between the presence of nitrite and the absence of nitrous acid under conditions of applied voltage and no applied voltage.
Nitrite (nitrite 100mg/L, NH) is arranged in the reactor 4 + 100 mg/L), the overall ammonia nitrogen removal effect becomes low when a voltage (0.4 v) is applied. Indicating that the voltage may inhibit the normal anammox process.
No nitrite is in the reactor, and the anaerobic ammonia oxidizing bacteria can not remove ammonia nitrogen under normal condition without applying voltage. About 10% ammonia nitrogen can be removed after voltage is applied. And the ammonia nitrogen removal efficiency gradually rises along with the increase of the acclimation time in the later stage.
Example 10
The invention also compares CV diagrams (figure 8) of the anaerobic ammonium oxidation bacteria domesticated for two months with those of the non-domesticated anaerobic ammonium oxidation bacteria, and the electrical activity of the domesticated anaerobic ammonium oxidation bacteria is better.

Claims (7)

1. An enrichment method of electroactive anaerobic ammonium oxidation bacteria, wherein a matched device comprises a water inlet system, an anaerobic ammonium oxidation microorganism electrochemical reactor and a water outlet system;
the water inlet system injects the aerated wastewater into the anaerobic ammonia oxidation microbial electrochemical reactor for treatment;
the anaerobic ammonia oxidation microbial electrochemical reactor is internally provided with three electrodes comprising a reference electrode, an anode and a cathode, wherein the three electrodes are positioned in the wastewater in the treatment process; the external part is provided with a constant potential rectifier connected with the internal three electrodes, and the bottom part is provided with a magnetic stirring system;
the water outlet system discharges treated wastewater from the anaerobic ammonia oxidation microbial electrochemical reactor; the method is characterized in that under the conditions that ammonia nitrogen exists in a substrate and nitrite does not exist, anaerobic ammonia oxidation sludge is added, the applied voltage is not more than 0.6v, an anaerobic ammonia oxidation microorganism electrochemical reactor is operated, and electroactive anaerobic ammonia oxidation bacteria are obtained through enrichment, wherein the electroactive anaerobic ammonia oxidation bacteria comprise: anammox bacteria;
the wastewater treated in the anaerobic ammonia oxidation microbial electrochemical reactor is aerated to remove dissolved oxygen in the wastewater; keeping dissolved oxygen below 0.05mg/L, and adjusting pH to 6.8-7.2;
placing the anaerobic ammonia oxidation microbial electrochemical reactor in an anaerobic environment at the temperature of 30 +/-5 ℃;
the anaerobic ammonia oxidation microbial electrochemical reactor is operated intermittently in batches, HRT is 24-72h, and 1/2-1/4 volume of solution is replaced in 8-16 h.
2. The method of claim 1, wherein the three electrodes use graphite felt as a working electrode and an auxiliary electrode, and a silver chloride electrode as a reference electrode.
3. The method of claim 2, wherein the anode graphite felt has an area of 4-16cm 2 The area of the cathode graphite felt is 36-64cm 2 The thickness is 0.5cm +/-0.05 cm.
4. The method of claim 1, wherein the water intake system comprises an anaerobic feed vessel, and a first aeration bottle, a barometric pressure stabilization bag, and a water intake peristaltic pump connected to the anaerobic feed vessel, respectively; the anaerobic feed supplement container is filled with wastewater to be treated, the first aeration bottle is connected to the wastewater and aerates the wastewater, the air pressure stabilizing bag is connected to the upper part of the liquid level of the wastewater to adjust air pressure, and the water inlet peristaltic pump is connected to the wastewater and is controlled by the water inlet controller to convey the wastewater to the anaerobic ammonia oxidation microorganism electrochemical reactor.
5. The process of claim 1, wherein the anaerobic ammonia oxidation microbial electrochemical reactor is further provided with a second aeration cylinder connected to the wastewater for aeration of the wastewater and a double-valve side hydrogen collecting device connected above the wastewater level for collecting gas generated from the treatment of the wastewater for pressure adjustment.
6. The method according to claim 1, wherein the effluent system comprises an effluent peristaltic pump and a water storage tank, wherein the effluent peristaltic pump is connected to the wastewater of the anammox microbial electrochemical reactor and is controlled by an effluent controller to convey the wastewater into the water storage tank.
7. A method for removing ammonia nitrogen, which is characterized in that the method is continuously operated after the enrichment is finished by directly using the enrichment method of any one of claims 1 to 6.
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