CN113655052B - 基于高活性MgO对四环素类抗生素可视化检测的方法 - Google Patents

基于高活性MgO对四环素类抗生素可视化检测的方法 Download PDF

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CN113655052B
CN113655052B CN202110887308.2A CN202110887308A CN113655052B CN 113655052 B CN113655052 B CN 113655052B CN 202110887308 A CN202110887308 A CN 202110887308A CN 113655052 B CN113655052 B CN 113655052B
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tetracycline
tetracycline antibiotics
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CN113655052A (zh
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冯静
曹乃文
高明明
魏彤
范壮军
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Harbin Engineering University
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Abstract

本发明提供一种用高活性MgO对四环素类抗生素可视化检测的方法。属于化学分析检测技术领域。将高活性MgO放入可能含有四环素类抗生素的水中,在可见光或者紫外光照射下,高活性MgO与四环素类抗生素发生络合反应,生成具有颜色的配合物,通过颜色的变化检测是否含有四环素,颜色越深,四环素类抗生素浓度越高。此外,通过离心干燥得到MgO粉末,与标准色卡比较颜色,能粗略分析四环素浓度。本发明利用高活性MgO,实现对四环素类抗生素简便、低成本和快速检测。

Description

基于高活性MgO对四环素类抗生素可视化检测的方法
技术领域
本发明属于化学分析检测技术领域,具体介绍了一种基于高活性MgO对四环素类抗生素可视化检测的方法。
背景技术
广谱抗生素中四环素类抗生素(主要包括金霉素、四环素和土霉素等)是目前使用最广泛,用量最大的抗生素之一。因其价格便宜抗菌效果好,广泛应用于医疗尤其是畜牧业当中。环境中四环素主要来源于医药企业排放的污水,因过期丢弃的药品、病人的新陈代谢以及农林畜牧业等。环境中高浓度四环素类抗生素对自然界、动物、植物和人类构成严重威胁。因此,开发一种价格低,无毒,有效、环保的对四环素类抗生素进行检测的方法,仍然是提供饮用水和环境保护的全球需求。
目前常见的四环素检测方法有:高效液相色谱法、液相-质谱联用法、微生物检测法,荧光检测法、免疫检测法,酶联免疫法和分光光度法等。其中高效液相色谱法、液相-质谱联用法和荧光检测法需要大型精密设备,价格昂贵,携带不方便。酶联免疫法和分光光度法交叉性强,容易被其他物质干扰,出现假阳性。因此,开发一种快速、简便、成本低的四环素类检测方法是很有必要的。
发明内容
本发明的目的是为了克服现有技术的缺陷而提供一种基于高活性MgO对四环素类抗生素可视化检测的方法。
本发明的目的是这样实现的:
一种基于高活性MgO对四环素类抗生素可视化检测的方法,包括如下步骤:
首先,将氧化镁在氢气/氩气气氛或者氩气气氛,高温500-900℃下活化2h~5h,得到高活性MgO粉末;
其次,将100mg高活性MgO投入到100mL可能含有四环素类抗生素的废水中,使用可见光或紫外光进行照射21-50分钟,促进高活性MgO和四环素发生络合反应,这时白色氧化镁颜色会发生变化,废水中四环素类抗生素浓度越高,MgO颜色越深,从而达到高活性MgO对四环素类抗生素的可视化检测。
所述四环素类抗生素为四环素、土霉素和金霉素中的一种或几种;
所述四环素类抗生素浓度为0-1000mg/L。
此外,将氧化镁负载到白色试纸上,即可制成四环素检测试纸。利用试纸表面MgO与四环素络合反应得到不同颜色的络合物,通过与标准色卡来对比,判断水中是否存在四环素并粗略分析四环素浓度。
与现有技术相比,本发明的有益效果是:
本发明高活性氧化镁能够与四环素快速发生显色络合反应。氧化镁价格便宜,无毒,来源丰富,经高温活化后,氧化镁表面容易暴露大量高活性的高活性镁,更容易与四环素发生配位反应。该方法具有操作简便、成本低、检测时间短、灵敏度高等优点,是一种非常有前景的抗生素检测方法。
附图说明
图1是镁与四环素类抗生素发生配位原理图;
图2(a)用高活性MgO检测0~50mg/L四环素后的粉末照片和(b)标准色卡。
具体实施方式
下面结合附图与具体实施方式对本发明作进一步详细描述。
实施方式一
本发明实施例1:定性检测水是否含有四环素类抗生素。取氧化镁在600℃下,氩气气氛中处理三个小时,得到高活性氧化镁。将0.1g上述产品放入到100mL可能含有四环素类抗生素的水中,使用300W氙灯进行照射30min。照射结束后,粉末由白色变为黄色或者棕色,则表示溶液含有四环素类抗生素,颜色越深,浓度越大。
实施方式二
本发明实施例2:粗略检测水中四环素类抗生素浓度。取氧化镁在600℃下,氩气气氛中处理三个小时,得到高活性氧化镁。将0.1g上述产品放入到100mL可能含有四环素类抗生素的水中,使用300W氙灯进行照射30min。照射结束后,将粉末离心,干燥。将干燥好的MgO粉末与我们提供标椎色卡进行对比,可以粗略定量四环素类抗生素浓度。
本发明公开了一种用高活性MgO对四环素类抗生素可视化检测的方法。属于化学分析检测技术领域。将高活性MgO放入可能含有四环素类抗生素的水中,在可见光或者紫外光照射下,高活性MgO与四环素类抗生素发生络合反应,生成具有颜色的配合物,通过颜色的变化检测是否含有四环素,颜色越深,四环素类抗生素浓度越高。此外,通过离心干燥得到MgO粉末,与标准色卡比较颜色,能粗略分析四环素浓度。本发明利用高活性MgO,实现对四环素类抗生素简便、低成本和快速检测。

Claims (3)

1.一种基于高活性MgO对四环素类抗生素可视化检测的方法,其特征在于:包括如下步骤:
首先,将氧化镁在氢气/氩气气氛或者氩气气氛,高温500-900℃下活化2h~5h,得到高活性MgO粉末;
其次,将100mg高活性MgO投入到100mL可能含有四环素类抗生素的废水中,使用可见光或紫外光进行照射21-50分钟,促进高活性MgO和四环素发生络合反应,这时白色氧化镁颜色会发生变化,废水中四环素类抗生素浓度越高,MgO颜色越深,从而达到高活性MgO对四环素类抗生素的可视化检测。
2.根据权利要求1所述的基于高活性MgO对四环素类抗生素可视化检测的方法,其特征在于:所述四环素类抗生素为四环素、土霉素和金霉素中的一种或几种。
3.根据权利要求1所述的基于高活性MgO对四环素类抗生素可视化检测的方法,其特征在于:所述四环素类抗生素浓度为0-1000mg/L。
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US4126680A (en) * 1977-04-27 1978-11-21 Pfizer Inc. Tetracycline antibiotic compositions
EP0271374A2 (fr) * 1986-12-10 1988-06-15 So.Ge.Val S.A. Composition antibiotique aqueuse à usage vétérinaire
CN103033502A (zh) * 2012-11-16 2013-04-10 陕西理工学院 一种鸡蛋清中残留土霉素药品的快速检测方法
CN107064040A (zh) * 2017-06-23 2017-08-18 江苏省环境科学研究院 水环境中痕量抗生素的高效富集与分离方法
CN107159094A (zh) * 2017-05-26 2017-09-15 湖南农业大学 磁性氢氧化镁吸附剂去除废水中四环素的方法

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Publication number Priority date Publication date Assignee Title
US4126680A (en) * 1977-04-27 1978-11-21 Pfizer Inc. Tetracycline antibiotic compositions
EP0271374A2 (fr) * 1986-12-10 1988-06-15 So.Ge.Val S.A. Composition antibiotique aqueuse à usage vétérinaire
CN103033502A (zh) * 2012-11-16 2013-04-10 陕西理工学院 一种鸡蛋清中残留土霉素药品的快速检测方法
CN107159094A (zh) * 2017-05-26 2017-09-15 湖南农业大学 磁性氢氧化镁吸附剂去除废水中四环素的方法
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