CN113648503B

CN113648503B - Sleep interference device for rats and mice and use method thereof

Info

Publication number: CN113648503B
Application number: CN202110795322.XA
Authority: CN
Inventors: 李清清; 王潇; 武莹莹
Original assignee: Guangzhou Medical University
Current assignee: Guangzhou Medical University
Priority date: 2021-07-14
Filing date: 2021-07-14
Publication date: 2023-02-24
Anticipated expiration: 2041-07-14
Also published as: CN113648503A

Abstract

The invention belongs to the technical field of experimental instruments, and particularly relates to a sleep interference device for rats and mice and a use method thereof, wherein the sleep interference device comprises a machine body and a composite moving device, and is characterized in that: the machine body is provided with a base, pressure sensors which are uniformly distributed are arranged on the base, a touch screen is arranged on the pressure sensors, the composite moving device comprises a linear power device and a poking rod power device, the linear power device is fixed on one side of the base, the linear power device comprises a first motor, a first speed reducer and a first screw rod, and the first motor is connected with the first screw rod through the first speed reducer. The invention can avoid the water invasion stimulation, the incapability of accurately interfering the sleep, the sports fatigue, the noise interference and the like of the existing device, realize the accurate interference, adopt different interference to different experimental bodies, simultaneously improve the sanitary conditions and improve the accuracy of the experiment, and the using method is simple and practical, and effectively realizes the sleep interference experiment on the rats and the mice.

Description

Sleep interference device for rats and mice and use method thereof

Technical Field

The invention belongs to the technical field of experimental instruments, and particularly relates to a sleep interference device for rats and mice and a using method thereof.

Background

Big and small mouse sleep deprivation appearance is the required behavioural instrument of research recovery brain, physical power, and sleep disorder can influence brain thinking to lead to the disease to take place, thereby make the human body shift to sub-health from health status, even disease state, in order to research sleep disorder and to develop antifatigue medicine or health products, need earlier stage animal model experiment, therefore sleep deprivation equipment is essential instrument.

The following are basically used in the existing sleep deprivation devices.

1) The experimental mouse is driven in the rotary cylinder mode to deprive the experimental mouse of sleep, theoretically, the experimental mouse has a long rest time when the rotating speed is low, intermittent sleep deprivation is caused, and the selection interval of the sleep deprivation mode is shortened when the rotating speed is high.

2) The rotating cage mode is that the rotating cage is driven by the motor to continuously rotate, and the experimental animal therein runs along with the rotating cage passively so as to achieve the purpose of depriving sleep.

3) Light touch stimulation method: that is, when the experimenter observes or observes through brain wave monitoring that the mouse enters a sleep state, the mouse is stimulated through sound, light, touch and the like to keep clear-headed. But this method is only suitable for short sleep deprivations.

4) Drug sleep deprivation: that is, mice are injected with drugs at regular time for deprivation, but there are disadvantages in that the mice are individually different, the effects and degree of sleep deprivation are difficult to quantify, and drug interference factors are increased.

5) Sleep deprivation in a horizontal table mode is mostly modified on an animal running table instrument and is mainly used for deprivation of sleep disorder (REM Sle mark) in the rapid eye movement period, and animals are easy to infect and die under the condition of long-time experiment deprivation. In addition, the interference among the platform type sleep deprivation experimental animals is also large, the instruments are heavy, and the noise is large.

6) Sleep deprivation method by depriving the rods: for example, CN209120922U discloses an experimental animal sleep deprivation device, which adopts a structure of a sleep deprivation rod and an electric push rod, wherein the sleep deprivation rod moves back and forth at the bottom of a transparent box body, so that the experimental animal cannot stay in place for rest all the time, and the body of the experimental animal must be actively moved or the sleep deprivation rod must be spanned, thereby achieving the effect of disturbing the sleep of the experimental animal. That is, mice were perturbed in standard laboratory cages with intermittent back and forth movement of deprivation rod sweeping stimulation, this approach prevented human contact and intervention from affecting sleep deprivation, and minimized physical activity throughout the sleep deprivation process. Is a method with higher acceptance degree at present. However, even the fifth sleep deprivation method has a certain defect, and although it does not require the animal to be in a walking state all the time, the period-adjustable deprivation rod scans the bottom of the rearing cage back and forth, but the precise deprivation cannot be achieved, and the animal may enter a sleep state within the period.

In summary, some of the existing sleep deprivation experimental devices for rats and mice have disadvantages, such as water invasion stimulation, sleep deprivation, and periodic failure to accurately interfere with the sleep of the rats and mice, and the interference devices still interfere with the normal activities of the rats and mice, so that exercise fatigue is easily caused, the running sound of the devices is loud, the caused noise interference is caused, and the accuracy of the experimental result is finally affected.

Disclosure of Invention

The invention aims to provide an instrument for sleep interference of animals in a mild mode without training the animals, and the instrument can resist sleep at 360 degrees without dead angles and can realize accurate interference.

The technical scheme of the invention is as follows:

the utility model provides a be used for big mouse sleep to disturb device, includes organism and compound mobile device, its characterized in that: the machine body is provided with a base, pressure sensors which are uniformly distributed are arranged on the base, a touch screen is arranged on the pressure sensors, the composite moving device comprises a linear power device and a poking rod power device, the linear power device is fixed on one side of the base, the linear power device comprises a first motor, a first speed reducer and a first screw rod, the first motor is connected with the first screw rod through the first speed reducer, and a fence is further arranged on the periphery of the base;

stir stick power device and include motor two, reduction gear two, lead screw two, guide rail, stir the stick, motor two connects lead screw two through reduction gear two, lead screw one is mutually perpendicular with lead screw two, two one ends of lead screw still are equipped with special-shaped nut and connect lead screw one, the base opposite side is located to the guide rail, the guide rail is parallel with linear power device, two other ends of lead screw are connected with the guide rail, be equipped with the guide way on the lead screw two, the guide way both sides are equipped with rectangle hole net one and rectangle hole net two, the touch-sensitive screen constitutes sealed cage structure with base, rectangle hole net one, rectangle hole net two, stir the stick and be located the cage and connect on lead screw two.

The pressure sensor fixing seat is used for fixing the pressure sensor, the adjustable time signal timer and the programming automatic controller are further arranged between the touch screen and the base, the pressure sensor, the touch screen, the adjustable time signal timer and the programming automatic controller are electrically connected in sequence, and the first motor and the second motor are respectively electrically connected with the programming automatic controller.

Specifically, the machine body is further provided with a rectangular mesh moving device which comprises a first chain wheel, a second chain wheel and a chain, one end of the first rectangular mesh and one end of the second rectangular mesh are respectively fixed on shafts of the first chain wheel and the second chain wheel, and the other ends of the first rectangular mesh and the second rectangular mesh are respectively fixed on two sides of the guide groove.

Preferably, the rectangular mesh moving device is further provided with a chain tensioning device.

The machine body is further provided with an access door device, the access door device comprises a first sliding door, a second sliding door and an access passage, the access passage is communicated with the cage and the outside, and the bottom surface of the access passage and the surface of the touch screen are positioned on the same horizontal plane.

Preferentially, the organism still is equipped with watering device and food device, watering device includes pond and water receiving basin, the pond is located on rail one side, and the water receiving basin is fixed in on the base inner wall, the pond is equipped with the drip tube with the water receiving basin and is connected, the food device is including eating the basin and connecing the basin, it is fixed in pond one side to eat the basin, it is fixed in water receiving basin one side to connect the basin, eat the basin and connect and be equipped with the food pipe between the basin and connect and be connected.

Preferably, a combined monitoring device is arranged in the machine body, and the combined monitoring device comprises a humidity monitoring device, a temperature monitoring device, an air monitoring device, a sound monitoring device and a camera monitoring device.

The use method of the device for disturbing the sleep of the rats and mice is characterized in that the machine body with the structure comprises the following steps:

s1, a mouse is placed on a touch screen (12) and is placed in a sealed cage which is formed by a base (1), a first rectangular mesh (51) and a second rectangular mesh (52);

when the sliding door I (8) and the sliding door II (9) are opened simultaneously, big and small mice enter the sleep deprivation instrument through the access passage (10), when the sliding door I (8) is closed by opening the sliding door II (9), the big and small mice enter the access passage (10) from the sleep deprivation instrument, and when the sliding door I (8) is opened and the sliding door II (9) is closed, the big and small mice leave the sleep deprivation instrument from the access passage (10), so that the big and small mice can conveniently enter and exit;

s2, the big mouse and the small mouse move on the touch screen (12), when the big mouse and the small mouse do not move or sleep on the touch screen (12), namely the static state exceeds 5 seconds, the signal that the big mouse and the small mouse do not move on the touch screen (12) inputs the signal that the big mouse and the small mouse do not move into the adjustable time signal timer (13) through the pressure sensor (11), the signal output from the adjustable time signal timer (13) is input into the programmable automatic controller (14), the signal output from the programmable automatic controller (14) is input into the composite moving device, the moving interference is carried out on the position of the big mouse and the small mouse which do not move through the movement of the toggle rod (45) in the composite moving device, the motionless or the sleep of the big mouse is interfered, and the big mouse and the small mouse start to move on the touch screen (12).

The organism device can be imported into through the program of manual work through programming automatic control ware (14), programming automatic control ware (14) with pressure sensor (11), touch-sensitive screen (12), adjustable time signal timer (13) signal processing, can realize automatic operation and make stir stick (45) can disturb each experimental body in the colony experimental body, and avoid different experimental animals to take the accurate interference of different times respectively automatically.

By using the drinking device (6) and the food device (7) on the body, the feeding of experimenters to rats and mice is facilitated, meanwhile, the sanitary condition is improved, and the rats and the mice are prevented from being infected and dead.

The combined monitoring device in the machine body is used for monitoring humidity, temperature, air, sound and shooting and recording the activity space of the machine body, the shooting device can monitor and record the experimental body for 24 hours, the experimental animal is guaranteed to have a free environment which is not interfered by the outside, and the accuracy of the experiment can be reflected.

The invention has the following beneficial effects:

1. this device is convenient for use in big mouse sleep deprivation experiment, can stably accomplish big mouse sleep deprivation experiment in controllable way, avoids defects such as the soaking amazing that exists among the prior art, the motion fatigue, noise interference, has solved artifical observation and artificial interference's uncertainty, avoids continuous training or disturbs big mouse and produces stress response, is favorable to improving the accuracy of experimental result, and

the full range of the experimental environment and the interference object can be monitored and recorded.

2. Can take different interference respectively to every experimental body in big mouse colony experiment body, that is to say the instrument can carry out sleep deprivation to many animals simultaneously to can dodge the experimental body automatically, and carry out automatic tracking interference and accurate sleep deprivation to the experimental body, just can be disturbed when just getting into or just getting into sleep state promptly to big mouse, forced movement stirs the stick and stops to remove during big mouse free activity, has avoided its reaction of overexcitation.

3. Make things convenient for big mouse business turn over and improve sanitary conditions through the business turn over door gear, guarantee the life demand of little animal through watering device and food device, unnecessary water is outside the appearance is deprived in sleep through the automatic discharge of outlet pipe of water receiving basin, unnecessary food is deprived in sleep through the automatic discharge of play esophagus of food receiving basin outside the appearance, humidity to the experimental body environment of moving about, the temperature, the air, sound carries out the monitoring record, camera device carries out 24 hours monitoring record to the experimental body, it has a free environment that does not receive external disturbance to have guaranteed the experimental animal, more can embody the accuracy of experiment.

4. The use method is simple and practical, and the sleep interference experiment on the big mouse and the small mouse is effectively realized.

Drawings

FIG. 1 is a schematic view of the structure of the present invention.

Fig. 2 is a schematic view of a side structure of the sliding door of the present invention.

Fig. 3 is a schematic view of a side structure of the food apparatus of the present invention.

Fig. 4 is a schematic structural diagram of an upper composite moving device according to the present invention.

Fig. 5 is a schematic cross-sectional view taken along line B-B in fig. 4.

Fig. 6 is a schematic cross-sectional view taken at D-D in fig. 5.

Fig. 7 is a schematic cross-sectional view taken at C-C in fig. 4.

Fig. 8 is a partially enlarged schematic view at E in fig. 7.

Fig. 9 is a schematic structural diagram of a pressure sensor and a touch screen on a base according to the present invention.

Fig. 10 isbase:Sub>A schematic cross-sectional view taken along linebase:Sub>A-base:Sub>A in fig. 9.

Fig. 11 is a schematic structural diagram of a composite moving device and a rectangular mesh moving device.

Fig. 12 is a schematic sectional view taken along line F-F in fig. 11.

FIG. 13 is a schematic view of the chain tensioner of the present invention.

Fig. 14 is a schematic structural view of an access door apparatus according to the present invention.

Fig. 15 is a top view of fig. 14.

FIG. 16 is a schematic view of the drinking device of the present invention.

Fig. 17 is a side view of fig. 16.

Fig. 18 is a schematic structural view of the food apparatus of the present invention.

Fig. 19 is a side view of fig. 18.

Fig. 20 is a schematic diagram of the combined monitoring of the present invention.

In the figure: 1-a base; 2-fencing; 3-a linear motion device; 4-a stick poking power device; 5-a rectangular mesh moving device; 6, a water drinking device; 7-a food device; 8, sliding the first door; 9-sliding a second door; 10-an access duct; 11-a pressure sensor; 12-a touch screen; 13-an adjustable time signal timer; 14-programming an automatic controller; 15-humidity monitoring means; 16-a temperature monitoring device; 17-air monitoring means; 18-a sound monitoring device; 19-probe of camera monitoring device; 20-pressure sensor fixing base; 21-a device cabinet; 31-a first motor; 32, a first speed reducer; 33-a first screw rod; 34-cushion one; 35-cushion two; 36-cushion III; 41-motor two; 42-a second speed reducer; 43-screw mandrel two; 44-a guide rail; 45-stirring the rod; 51-a rectangular mesh I; 52-rectangular mesh II; 53, a first chain wheel; 54-sprocket two; 55-chain; 56-chain tensioning device; 61-a pool; 62-a water dropping pipe; 63-a water receiving basin; 71-food bowl; 72-a food conduit; 73-food receiving basin; 431-heteromorphic spiro; 432-a guide groove; 433-self-lubricating bearing A; 434-a guide closing plate; 435-self-lubricating bearing B; 436-rolling wheels.

Detailed Description

In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientation or positional relationship indicated in the drawings for convenience in describing the invention and for simplicity in description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be considered limiting. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be connected internally or indirectly. The specific meanings of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.

The invention aims to provide an instrument for sleep deprivation of animals in a mild mode without training the animals, the instrument can resist sleep at 360 degrees without dead angles, can realize accurate deprivation, can interfere experimental animals with different sizes according to time, can also respectively interfere each experimental body in a group of experimental bodies at different times, and can automatically avoid the experimental animals.

The invention is described below with reference to the accompanying drawings:

as shown in fig. 1-7, a sleep disturbance device for rats and mice comprises a body and a composite moving device, and is characterized in that: the machine body is provided with a base 1, pressure sensors 11 which are uniformly distributed are arranged on the base 1, a touch screen 12 is arranged on the pressure sensors 11, the composite moving device comprises a linear power device 3 and a toggle rod power device 4, the linear power device 3 is fixed on one side of the base 1, the linear power device 3 comprises a motor I31, a speed reducer I32 and a screw rod I33, the motor I31 is connected with the screw rod I33 through the speed reducer I32, and a fence 2 is further arranged on the periphery of the base 1;

the poking rod power device 4 comprises a second motor 41, a second speed reducer 42, a second screw rod 43, a guide rail 44 and a poking rod 45, wherein the second motor 41 is connected with the second screw rod 43 through the second speed reducer 42, the first screw rod 33 is perpendicular to the second screw rod 43, a special-shaped nut 431 is further arranged at one end of the second screw rod 43 to be connected with the first screw rod 33, the other end of the second screw rod 43 is connected with the guide rail 44, the guide rail 44 is arranged on the other side of the base 1, the guide rail 44 is parallel to the linear power device 4, a guide groove 432 is arranged on the second screw rod 43, a first rectangular hole net 51 and a second rectangular hole net 52 are arranged on two sides of the guide groove 432, a sealed cage structure is formed by the touch screen 12, the base 1, the first rectangular hole net 51 and the second rectangular hole net 52, the poking rod 45 is located in the cage and connected to the second screw rod 43.

The base 1 is made of transparent synthetic plastic materials in one-step forming, observation of experimenters is facilitated, and the fence 2 is made of synthetic plastic materials in one-step forming. The pressure sensor fixing seat 20, the door access device, the water drinking device 6, the food device 7 and the device cabinet 21 are made of synthetic plastic materials, and the special-shaped nut 431 and the toggle rod 45 are made of nylon materials, so that the rats and mice are prevented from being injured.

As shown in fig. 8, the other end of the second screw rod 43 is provided with a self-lubricating bearing a 433, a guide closing plate 434, a self-lubricating bearing b 435 and a roller 436, the self-lubricating bearing a 433 is inlaid into a hole of the guide closing plate 434, the guide closing plate 434 is fixed at the other end of the second screw rod 43 through a screw, one end of the self-lubricating bearing b 435 is inserted into the guide closing plate 434, and one end of the self-lubricating bearing b 435 is inlaid into the roller 436, and the second screw rod 43 is fixed in the guide rail 44 through the roller 436.

As shown in fig. 7 and 10, a first buffer pad 34 and a second buffer pad 35 are further arranged in the machine body, a third buffer pad 36 is arranged at two ends of the guide rail 44, the first buffer pad 34, the second buffer pad 35 and the third buffer pad 36 are made of shock-absorbing rubber pads, so that mechanical vibration generated when the stirring rod 45 performs compound motion and moves in a rectangular mesh net can be effectively reduced, a sound-insulating effect is achieved, noise interference caused by big and small mice is avoided in an experiment, and the experiment is more accurate.

As shown in fig. 9, a pressure sensor fixing seat 20 for fixing the pressure sensor 11 is provided on the base 1, an adjustable time signal timer 13 and a programmable automatic controller 14 are further provided between the touch screen 12 and the base 1, the pressure sensor 11, the touch screen 12, the adjustable time signal timer 13 and the programmable automatic controller 14 are electrically connected in sequence, and the first motor 31 and the second motor 41 are respectively electrically connected to the programmable automatic controller 14.

Rotate to drive reduction gear 32 through motor 31 and rotate, reduction gear 32 is rotatory to drive lead screw 33 and is rotated, make lead screw two 43 do the longitudinal motion along lead screw one 31 through special-shaped nut 431, and then realize stirring the vertical linear motion of stick 45, motor two 41 is rotatory to drive reduction gear two 42 rotatory, reduction gear two 42 is rotatory to drive lead screw two 43 rotatory, make stirring stick 45 do the transverse motion along lead screw two 43, when linear motion device 3 and stirring stick power device 4 cooperation use, stir stick 45 can not have the compound movement of dead angle in the cage, can realize removing or stirring according to the actual response is required, and realize can be to the accurate sleep interference of big mouse in the experiment, also can adopt the interference of different time respectively to each experimental body in the colony experimental body, and avoid the experimental animal automatically.

The programming controller, the pressure sensor and the signal timer are all products in the prior art, and are applied to the design of the invention according to actual needs.

A method of using a sleep disruption device for rats and mice, comprising:

s1, when the mouse is used, a mouse is placed on the touch screen 12 and is placed in a sealed cage which is formed by the mouse, the base 1, the first rectangular mesh 51 and the second rectangular mesh 52;

s2, the big mouse and the small mouse move on the touch screen 12, when the big mouse and the small mouse do not move or sleep on the touch screen 12, namely the static state exceeds 5 seconds, the signal that the big mouse and the small mouse do not move on the touch screen 12 inputs the signal that the big mouse and the small mouse do not move into the adjustable time signal timer 13 through the pressure sensor 11, the signal output from the adjustable time signal timer 13 is input into the programmed automatic controller 14, the signal output from the programmed automatic controller 14 is input into the composite moving device, the big mouse and the small mouse which are inactive are interfered by moving the toggle rod 45 in the composite moving device to the position of the big mouse and the small mouse which are inactive, the motionlessness or the sleep of the big mouse is interfered, the big mouse starts to move on the touch screen 12, and the accurate interference is realized.

In addition, the rat and mouse sleep interference device can be imported through a manually programmed program through the programming automatic controller 14, the programming automatic controller 14 processes signals of the pressure sensor 11, the touch screen 12 and the adjustable time signal timer 13, and the automatic operation can be realized, so that the toggle rod 45 can interfere with each experimental body in a group experimental body, and accurate interference of different experimental animals at different times is automatically avoided.

The precise interference of the compound movement of the stirring rod 45 avoids the phenomenon that the experimental rat generates exercise fatigue and stress response by scanning back the experimental rat in the sleep deprivation method of the deprivation rod, so that the experimental data is more accurate.

As shown in fig. 12, the machine body is further provided with a rectangular mesh moving device 5, which includes a first chain wheel 53, a second chain wheel 54, and a chain 55, one end of the first rectangular mesh 51 and one end of the second rectangular mesh 52 are respectively fixed on the shafts of the first chain wheel 53 and the second chain wheel 54 by screws and are rolled up for several turns, the other end of the first rectangular mesh 51 and the second rectangular mesh 52 are respectively fixed on two sides of the guide groove 432, when the second lead screw 43 makes a linear motion along the first lead screw 33, the guide groove 432 drives the first rectangular mesh 51 or the second rectangular mesh 52 to move, the first rectangular mesh 51 drives the first chain wheel 53 to rotate, and the first chain wheel 53 rotates the second chain wheel 54 through the chain 55, thereby realizing the movement of the second rectangular mesh 52; or the second rectangular hole net 52 moves to enable the second chain wheel 54 to rotate, the second chain wheel 54 enables the first chain wheel 53 to rotate through the chain 55, and the first rectangular hole net 51 moves. Through rectangular hole net mobile device 5 for synchronous rectilinear motion is done to rectangular hole net one 51 and rectangular hole net two 52, has prevented that big or small mouse from losing the appearance top from the sleep and running off, and rectangular hole net makes things convenient for experimenter's observation big or small mouse simultaneously.

As shown in fig. 11 and 13, the rectangular mesh moving device 5 is further provided with a chain tensioning device 56, the tightness degree of the chain 55 is adjusted by the chain tensioning device 56, the chain 55 is prevented from loosening and falling off, the abrasion of the first chain wheel 53, the second chain wheel 54 and the chain 55 is reduced, and the operation stability and the service life of the device are ensured.

As shown in fig. 2, 14 and 15, the machine body is further provided with an access door device, the access door device comprises a first sliding door 8, a second sliding door 9 and an access passage 10, the access passage 10 is communicated with the cage and the outside, and the bottom surface of the access passage 10 and the surface of the touch screen 12 are on the same horizontal plane.

When the first sliding door 8 and the second sliding door 9 are opened simultaneously, the big mouse and the small mouse enter the sleep deprivation instrument through the access passage 10, when the second sliding door 9 is opened and the first sliding door 8 is closed, the big mouse enters the access passage 10 from the sleep deprivation instrument, when the first sliding door 8 is opened and the second sliding door 9 is closed, the big mouse and the small mouse leave the sleep deprivation instrument from the access passage 10, and the big mouse and the small mouse can conveniently enter and exit.

As shown in fig. 16-19, the machine body is further provided with a drinking device 6 and a food device 7, the drinking device 6 comprises a water tank 61 and a water receiving basin 63, the water tank 61 is arranged on one side of the fence 2, the water receiving basin 63 is fixed on the inner wall of the base 1, the water tank 61 and the water receiving basin 63 are provided with a water dripping pipe 62 for connection, the food device 7 comprises a food basin 71 and a food receiving basin 73, the food basin 71 is fixed on one side of the water tank 61, the food receiving basin 73 is fixed on one side of the water receiving basin 63, and a food pipeline 72 is arranged between the food basin 71 and the food receiving basin 73 for connection.

Water enters the water receiving basin 63 from the water pool 61 through the water dripping pipe 62, redundant water is automatically discharged through the pipeline of the water receiving basin 63, similarly, food enters the food receiving basin 73 from the food basin 71 through the food pipeline 72, and redundant food is automatically discharged through the pipeline of the food receiving basin 73. The feeding of experimenters to big mice is convenient, and the sanitary conditions are improved to avoid the death of the big mice due to infection.

As shown in fig. 20, a combined monitoring device is arranged in the machine body, the combined monitoring device includes a humidity monitoring device 15, a temperature monitoring device 16, an air monitoring device 17, a sound monitoring device 18 and a camera monitoring device 19, the machine body is further provided with a device cabinet 21 for fixing the combined monitoring device, the humidity monitoring device 15, the temperature monitoring device 16, the air monitoring device 17, the sound monitoring device 18 and the camera monitoring device 19 are respectively installed in the device cabinet 21 and fixed on a side plate of the device cabinet 21 by screws, and the device cabinet 21 is fixed on a bottom plate inside the enclosure 2 by bolts, wherein probes of the humidity monitoring device 15, the temperature monitoring device 16, the air monitoring device 17, the sound monitoring device 18 and the camera monitoring device 19 penetrate through the wall of the base 1 and are placed in the moving space of the rat and used for humidity monitoring, temperature monitoring, air monitoring, sound monitoring and camera monitoring and recording of the moving space of the rat, and the camera monitoring device can perform 24-hour monitoring and recording on an experimental body, thereby ensuring that the experimental animal has a free environment without external interference and more accurate experiment can be embodied.

The invention has the following beneficial effects:

1. can realize accomplishing big mouse sleep deprivation experiment in stable controllable ground, avoid soaking defects such as amazing, motion tired, noise interference that exist among the prior art, solve artifical observation and artificial interference's uncertainty, avoid lasting training or disturb big mouse and produce stress response, be favorable to improving the accuracy of experimental result to can monitor and record experimental environment and interfering object full range.

2. Can realize taking different interference respectively to every experimental body in big mouse colony experimental body, can sleep to many animals simultaneously and deprive to can dodge the experimental body automatically, and carry out automatic tracking interference and accurate sleep to the experimental body and deprive, only big mouse just can be disturbed when entering or just entering sleep state promptly, and the stick that dials during big mouse free activity stops to remove, has avoided its stress response.

3. Can realize making things convenient for the business turn over of big mouse and improve sanitary conditions through business turn over door device, guarantee the life demand of toy through watering device and food device, unnecessary water is outside the appearance is deprived in sleep through the automatic discharge of outlet pipe of water receiving basin, unnecessary food is outside the appearance is deprived in sleep through the automatic discharge of play esophagus of receiving the basin, humidity to the experimental body environment of moving about, the temperature, the air, sound carries out the monitoring record, camera device carries out 24 hours monitoring record to the experimental body, it has a free environment that does not receive external disturbance to have guaranteed the experimental animal, the accuracy that can more physically test.

4. The use method is simple and practical, and the sleep interference experiment aiming at the big mouse and the small mouse is effectively realized.

Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction. Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.

Claims

1. The utility model provides a be used for big mouse sleep to disturb device, includes organism and compound mobile device, its characterized in that: the machine body is provided with a base (1), pressure sensors (11) which are uniformly distributed are arranged on the base (1), a touch screen (12) is arranged on the pressure sensors (11), the composite moving device comprises a linear power device (3) and a toggle rod power device (4), the linear power device (3) is fixed on one side of the base (1), the linear power device (3) comprises a first motor (31), a first speed reducer (32) and a first screw rod (33), the first motor (31) is connected with the first screw rod (33) through the first speed reducer (32), and a fence (2) is further arranged on the periphery of the base (1);

the poking rod power device (4) comprises a second motor (41), a second speed reducer (42), a second screw rod (43), a guide rail (44) and a poking rod (45), the second motor (41) is connected with the second screw rod (43) through the second speed reducer (42), the first screw rod (33) is perpendicular to the second screw rod (43), a special-shaped nut (431) is further arranged at one end of the second screw rod (43) to be connected with the first screw rod (33), the guide rail (44) is arranged on the other side of the base (1), the guide rail (44) is parallel to the linear power device (3), the other end of the second screw rod (43) is connected with the guide rail (44), a guide groove (432) is formed in the second screw rod (43), a first rectangular hole net (51) and a second rectangular hole net (52) are arranged on two sides of the guide groove (432), the touch screen (12) and the base (1), the first rectangular hole net (51) and the second rectangular hole net (52) form a cage structure, and the poking rod (45) is located in the sealed cage;

the machine body is further provided with an access door device, the access door device comprises a first sliding door (8), a second sliding door (9) and an access passage (10), the access passage (10) is communicated with the cage and the outside, and the bottom surface of the access passage (10) and the surface of the touch screen (12) are on the same horizontal plane;

a combined monitoring device is arranged in the machine body and comprises a humidity monitoring device (15), a temperature monitoring device (16), an air monitoring device (17), a sound monitoring device (18) and a camera monitoring device (19).

2. The sleep disturbance device for rats and mice according to claim 1, wherein: the pressure sensor fixing seat (20) for fixing the pressure sensor (11) is arranged on the base (1), an adjustable time signal timer (13) and a programming automatic controller (14) are further arranged between the touch screen (12) and the base (1), the pressure sensor (11), the touch screen (12), the adjustable time signal timer (13) and the programming automatic controller (14) are sequentially electrically connected, and the motor I (31) and the motor II (41) are respectively electrically connected with the programming automatic controller (14).

3. The sleep disturbance device for rats and mice according to claim 1, wherein: the machine body is further provided with a rectangular mesh moving device (5) which comprises a first chain wheel (53), a second chain wheel (54) and a chain (55), one end of the first rectangular mesh (51) and one end of the second rectangular mesh (52) are respectively fixed on shafts of the first chain wheel (53) and the second chain wheel (54), and the other ends of the first rectangular mesh and the second rectangular mesh are respectively fixed on two sides of the guide groove (432).

4. A sleep disturbance device for rats and mice according to claim 3, characterized in that: the rectangular mesh moving device (5) is further provided with a chain tensioning device (56).

5. The sleep disturbance device for rats and mice according to claim 1, wherein: the organism still is equipped with watering device (6) and food device (7), watering device (6) are including pond (61) and water receiving basin (63), on rail (2) one side was located in pond (61), on water receiving basin (63) were fixed in base (1) inner wall, pond (61) were equipped with drip pipe (62) with water receiving basin (63) and were connected, food device (7) are including eating basin (71) and connect and eat basin (73), it is fixed in pond (61) one side to eat basin (71), it is fixed in water receiving basin (63) one side to connect and eat basin (73), it is connected with food pipeline (72) to eat basin (71) and connect and be equipped with between basin (73).

6. The use method of the device for disturbing sleep of mice and rats as claimed in any one of claims 1 to 5, wherein the body with the above structure is used, comprising the steps of:

when the sliding door I (8) and the sliding door II (9) are opened simultaneously, big and small mice enter the sleep deprivation instrument through the access passage (10), when the sliding door I (9) is opened and the sliding door I (8) is closed, the big and small mice enter the access passage (10) from the sleep deprivation instrument, and when the sliding door I (8) is opened and the sliding door II (9) is closed, the big and small mice leave the sleep deprivation instrument from the access passage (10), so that the big and small mice can conveniently enter and exit;

s2, the big mouse moves on the touch screen (12), when the big mouse does not move or sleep on the touch screen (12), namely the static state exceeds 5 seconds, the signal that the big mouse does not move on the touch screen (12) inputs the signal that the big mouse does not move into the adjustable time signal timer (13) through the pressure sensor (11), the signal output from the adjustable time signal timer (13) is input into the programmed automatic controller (14), the signal output from the programmed automatic controller (14) is input into the composite moving device, the moving interference is carried out on the position of the big mouse and the small mouse which are not moved through the movement of the poking rod (45) in the composite moving device, the motionless or the sleep of the big mouse is interfered, and the big mouse and the small mouse start to move on the touch screen (12).

7. The use method of the device for disturbing the sleep of the mice and rats according to claim 6, wherein the machine body device can be imported through a manually programmed program through the automatic programming controller (14), and the automatic programming controller (14) processes signals of the pressure sensor (11), the touch screen (12) and the adjustable time signal timer (13) to realize automatic operation so that the stirring rod (45) can disturb each experimental body in a group of experimental bodies and automatically avoid accurate disturbance of different experimental animals at different times respectively.

8. The use method of the device for interfering the sleep of the big mouse and the small mouse as claimed in claim 6 is characterized in that the water drinking device (6) and the food device (7) on the body are used, so that the experimenter can conveniently feed the big mouse and the small mouse, and meanwhile, the sanitary condition is improved, and the death of the big mouse and the small mouse due to infection is avoided;

use the internal combination monitoring device of above-mentioned organism for carry out humidity control, temperature monitoring, air monitoring, sound control and the control of making a video recording and record to its activity space, camera device can carry out 24 hours control record to the experimental body, has guaranteed that experimental animals has a free environment that does not receive external disturbance, more can embody the accuracy of experiment.