CN113647907A

CN113647907A - Laboratory mouse intestinal sensitivity test equipment

Info

Publication number: CN113647907A
Application number: CN202110901867.4A
Authority: CN
Inventors: 李建新; 林允
Original assignee: Wenzhou Peoples Hospital
Current assignee: Wenzhou Peoples Hospital
Priority date: 2021-08-06
Filing date: 2021-08-06
Publication date: 2021-11-16

Abstract

The invention discloses a device for testing the sensitivity of an intestinal tract of a laboratory mouse, which adopts the technical scheme that the device comprises a mounting seat fixedly connected to a placing plate, wherein an inflator pump is mounted on the mounting seat, the front end of the inflator pump is connected with an inflation tube, one end of the inflation tube is connected with an inflation ball, a tube body close to the inflation ball is provided with a sensor for monitoring the intestinal tract and internal organs, and the sensor is electrically connected with a monitor. The invention has the following beneficial effects: one end of an inflation tube and an uninflated inflation ball are inserted into the intestinal tract of a laboratory mouse, the inflation tube inflates and expands the inflation ball, and a monitor reads data of the intestinal tract and the viscera monitored by a sensor, so that rectal dilatation experiments can be performed on the mouse, and the sensitivity of the intestinal tract of the mouse can be measured.

Description

Laboratory mouse intestinal sensitivity test equipment

Technical Field

The invention relates to animal experimental equipment in basic research, in particular to experimental mouse intestinal sensitivity testing equipment.

Background

The animal is a good experimental model which can simulate human behavior and physiological function. Visceral hypersensitivity is one of the important pathophysiological features of irritable bowel syndrome. In basic research, animals are commonly used to establish a model for irritable bowel syndrome. Only by detecting the intestinal sensitivity of the animal, whether the animal model is successfully made can be judged, and the effectiveness of the model is verified. The rodent intestinal sensitivity is tested by performing rectal dilation test and scoring by evaluating abdominal contractile reflex behavior to determine the animal intestinal sensitivity.

The current instrument that detects is mostly the manual preparation of experimenter, and does not have the constraint effect to the animal, and experiment operator does not have the safety guarantee, receives the animal to bite easily, has scientific, normative, the not enough problem of homogeneity. Therefore, a sensitivity test experimental tool with animal fixing function, standard operation and simplicity is needed.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide the experimental rat intestinal sensitivity testing device which can be used for carrying out rectal distension experiments on rats so as to determine the rat intestinal sensitivity.

In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a laboratory mouse intestinal sensitivity test equipment, including placing the board, place and install the watch-dog on the board, spacing frame and aerating device, spacing frame is provided with the handle hole that holds the chamber and be used for exposing laboratory mouse hip that are used for holding the laboratory mouse, aerating device is including the mount pad of fixed connection on placing the board, install the inflator pump on the mount pad, the front end of inflator pump is connected with the gas tube, the one end of gas tube is connected with the inflatable ball, and be provided with the sensor that is used for monitoring intestinal and viscera on being close to the pipe shaft of inflatable ball, the sensor is connected with the watch-dog electricity.

The invention is further configured to: the top surface that holds the chamber is provided with the guide bar that the board was placed to opening and a plurality of perpendicular to, and the open-ended top is provided with the apron, is provided with a plurality of bullports on the apron, and a plurality of guide bars all pass corresponding bullports to threaded connection has the adjustable ring.

The invention is further configured to: the bottom surface of apron still is provided with the inductor that is used for monitoring laboratory mouse sign.

The invention is further configured to: the both sides of spacing frame are equallyd divide and are provided with two spacing holes that are used for supplying laboratory mouse four limbs to pass to the both sides top surface all is provided with two spacing grooves, and the apron is less than the opening size, and two connecting rods with spacing groove looks adaptation of the equal fixedly connected with in both sides, four connecting rods all set up in the top of corresponding spacing groove, and be connected with vertical dead lever, and the dead lever setting is in the outside of spacing frame, and the lower extreme all is connected with the spacing head of U-shaped.

The invention is further configured to: the top surface of apron is provided with spacing seat, and the top surface of spacing seat is provided with fixed slot and regulation hole to the top is provided with the clamp plate, and the bottom surface of clamp plate is provided with indent and through-hole, and indent and fixed slot correspond the setting from top to bottom, and through-hole threaded connection has the regulation pole, and the lower extreme of adjusting the pole passes the through-hole to with regulation hole threaded connection.

The invention is further configured to: the quantity of adjusting hole, through-hole and regulation pole is two, and two are adjusted the hole and are set up respectively in the both sides of fixed slot, and two through-holes set up respectively in the both sides of indent with adjusting the pole.

The invention is further configured to: the cover plate and the opening are rectangular.

The invention is further configured to: still sliding connection has the piston in the inflator pump to be provided with the scale on the barrel body, piston connection has the pole of inflating, is provided with the push pedal on the pole of inflating, and fixedly connected with gag lever post on the face of push pedal towards the inflator pump, and the place ahead of gag lever post is provided with the sliding sleeve, and the sliding sleeve cover is established on the inflator pump to with inflator pump sliding connection, and be provided with the locating hole, locating hole threaded connection has fastening screw, and fastening screw's one end is passed the locating hole, in order to be used for the butt on the inflator pump.

The invention is further configured to: the inflator can be detachably connected to the mounting seat, the placing plate is provided with an electric telescopic rod, and one end of the electric telescopic rod can be detachably connected to the pushing plate.

The invention is further configured to: the inflation tube is provided with a three-way reversing valve, the three-way reversing valve is provided with an exhaust hole and an electric commutator, the monitor is provided with a control center, and the control center is electrically connected with the electric commutator.

In conclusion, the invention has the following beneficial effects: the experimental mouse is placed into the containing cavity and bound, the butt of the experimental mouse is exposed at the operation hole, one end of the inflation tube and the uninflated inflation ball are inserted into the intestinal tract of the experimental mouse, the inflation tube inflates and expands the inflation ball, the monitor reads data of the intestinal tract and the viscera monitored by the sensor, and then rectal dilatation experiments can be completed on the mouse to determine the sensitivity of the intestinal tract of the mouse. The device can fix animals, is standard and simple in operation and is easy to be used for standardized experiments and teaching.

Drawings

FIG. 1 is a schematic view of a connection structure of an experimental mouse intestinal sensitivity test device;

FIG. 2 is a schematic view showing a connection structure of the inflation tube;

FIG. 3 is a schematic view of a connection structure of a position-limiting frame;

fig. 4 is a schematic view of a connection structure of the limiting seat.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings and examples. In which like parts are designated by like reference numerals. It should be noted that the terms "front," "back," "left," "right," "upper" and "lower" used in the following description refer to directions in the drawings, and the terms "bottom" and "top," "inner" and "outer" refer to directions toward and away from, respectively, the geometric center of a particular component.

Referring to fig. 1-4, a laboratory mouse intestinal sensitivity test device comprises a placing plate 1, a monitor 11, a limiting frame 2 and an inflating device 3 are mounted on the placing plate 1, the limiting frame 2 is provided with a containing cavity 21 for containing a laboratory mouse and an operation hole 22 for exposing the buttocks of the laboratory mouse, the inflating device 3 comprises a mounting seat 31 fixedly connected to the placing plate 1, an inflator 32 is mounted on the mounting seat 31, the front end of the inflator 32 is connected with an inflation tube 33, one end of the inflation tube 33 is connected with an inflation ball 34, a tube body close to the inflation ball 34 is provided with a sensor 35 for monitoring intestinal tracts and internal organs, and the sensor 35 is electrically connected with the monitor 11.

Through the scheme, a laboratory mouse is placed in the accommodating cavity 21 and is bound, the buttocks of the laboratory mouse is exposed at the operation hole 22, one end of the inflation tube 33 and the uninflated inflation ball 34 are inserted into the intestinal tract of the laboratory mouse, the inflation tube 33 inflates and expands the inflation ball 34 through the inflation tube 32, the monitor 11 reads data of the intestinal tract and the viscera monitored by the sensor 35, and then rectal dilatation experiments can be carried out on the mouse so as to determine the sensitivity of the intestinal tract of the mouse. The device can fix animals, is standard and simple in operation and is easy to be used for standardized experiments and teaching.

As a modified embodiment, the top surface of the receiving chamber 21 is provided with an opening 211 and a plurality of guide rods 212 perpendicular to the placing plate 1, a cover plate 23 is provided above the opening 211, a plurality of guide holes 231 are provided on the cover plate 23, and the plurality of guide rods 212 each pass through the corresponding guide hole 231 to be screw-coupled with an adjusting ring 213.

Through above-mentioned scheme, guide rod 212 and guide hole 231 cooperation for the steady pressure of apron 23 is in the top of laboratory mouse, and fixes through adjustable ring 213, thereby makes the laboratory mouse can the stable posture of holding, can not follow-up, so that carry out the experiment.

As a modified specific embodiment, the bottom surface of the cover plate 23 is also provided with a sensor 232 for monitoring the signs of the laboratory mouse.

Through above-mentioned scheme, inductor 232 laminates at the back of laboratory mouse, and external monitoring laboratory mouse sign cooperates internal monitoring, and then obtains better monitoring effect.

As a modified embodiment, two limiting holes 213 for four limbs of the laboratory mouse to pass through are respectively arranged on two sides of the limiting frame 2, two limiting grooves 214 are arranged on the top surfaces of the two sides, the cover plate 23 is smaller than the opening 211, two connecting rods 233 matched with the limiting grooves 214 are fixedly connected on the two sides, the four connecting rods 233 are arranged above the corresponding limiting grooves 214 and are connected with vertical fixing rods 234, the fixing rods 234 are arranged on the outer side of the limiting frame 2, and the lower ends of the fixing rods are connected with U-shaped limiting heads 235.

Through above-mentioned scheme, laboratory mouse's four limbs pass corresponding spacing hole 213, and apron 23 pushes down, and connecting rod 233 inserts corresponding spacing groove 214 in, and spacing head 235 further fixes laboratory mouse's four limbs, strengthens the restriction to laboratory mouse, guarantees the safety of experiment.

As a modified specific embodiment, the top surface of the cover plate 23 is provided with a limit seat 24, the top surface of the limit seat 24 is provided with a fixing groove 241 and an adjusting hole 242, and the upper side is provided with a pressing plate 25, the bottom surface of the pressing plate 25 is provided with a pressing groove 251 and a through hole 252, the pressing groove 251 and the fixing groove 241 are arranged corresponding to each other up and down, the through hole 252 is in threaded connection with an adjusting rod 253, and the lower end of the adjusting rod 253 passes through the through hole 252 to be in threaded connection with the adjusting hole 242.

Through above-mentioned scheme, place spacing seat 24's fixed slot 241 with laboratory mouse's tail on, cover clamp plate 25, fixed slot 241 and indent 251 are spacing with the tail, rotate the regulation pole 253, with spacing seat 24 and clamp plate 25 fixed connection to it is fixed with the tail, thereby keep laboratory mouse anus to expose, so that operate.

As a modified specific embodiment, the number of the adjusting holes 242, the through holes 252 and the adjusting rods 253 is two, two adjusting holes 242 are respectively disposed at both sides of the fixing groove 241, and two through holes 252 and the adjusting rods 253 are respectively disposed at both sides of the pressing groove 251.

Through above-mentioned scheme, double thread connection can further strengthen fixedly, guarantees fixed effect.

As a modified embodiment, the cover plate 23 and the opening 211 are rectangular.

Through the scheme, the shapes are similar, the processing and the operation are convenient, and the effect of matching and limiting is achieved.

As an improved specific embodiment, a piston 321 is further slidably connected in the inflator 32, a graduated scale is arranged on the inflator body, the piston 321 is connected with an inflator rod 322, a push plate 323 is arranged on the inflator rod 322, a limit rod 324 is fixedly connected to a face, facing the inflator 32, of the push plate 323, a sliding sleeve 36 is arranged in front of the limit rod 324, the sliding sleeve 36 is sleeved on the inflator 32 to be slidably connected with the inflator 32, and a positioning hole 361 is arranged, a fastening screw 362 is threadedly connected to the positioning hole 361, and one end of the fastening screw 362 penetrates through the positioning hole 361 to be abutted against the inflator 32.

Through above-mentioned scheme, remove sliding sleeve 36 according to the scale, rotate fastening screw 362, it is fixed with sliding sleeve 36, when admitting air, promote push plate 323, gag lever post 324 pushes up on sliding sleeve 36 to the propulsive piston 321 position of accurate control, with the air input of accurate estimation rush-in, with the inflation size and the atmospheric pressure of estimation balloon 34, many times or gradient experiment, and then the cooperation monitoring data obtains more accurate experimental result.

As a modified specific embodiment, the inflator 32 is detachably connected to the mounting seat 31, the electric telescopic rod 12 is mounted on the placing plate 1, and one end of the electric telescopic rod 12 is detachably connected to the pushing plate 323.

Through the scheme, the electric telescopic rod 12 accurately controls the feeding air quantity, and the accuracy of an experiment is ensured. The air cylinder 32 is removably replaceable to raise the upper limit of the intake air amount and the upper limit of the intake air speed.

As a modified specific embodiment, the inflation tube 33 is provided with a three-way reversing valve 331, the three-way reversing valve 331 is provided with an exhaust hole 332 and an electric diverter 333, the monitor 11 is provided with a control center 13, and the control center 13 is electrically connected with the electric diverter 333.

Through the scheme, the control center 13 controls the electric commutator 333 to adjust the three-way reversing valve 331 so as to realize sealing, air inlet and air outlet, and the use is convenient.

The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that modifications and embellishments within the scope of the invention may occur to those skilled in the art without departing from the principle of the invention, and are considered to be within the scope of the invention.

Claims

1. The utility model provides a laboratory mouse intestinal sensitivity test equipment, is including placing board (1), characterized by: place and install watch-dog (11), spacing frame (2) and aerating device (3) on board (1), spacing frame (2) are provided with the handle hole (22) that holds chamber (21) and be used for exposing the laboratory mouse butt that are used for holding the laboratory mouse, aerating device (3) are including mount pad (31) of fixed connection on placing board (1), install inflator pump (32) on mount pad (31), the front end of inflator pump (32) is connected with gas tube (33), the one end of gas tube (33) is connected with inflatable ball (34) to be provided with on the pipe shaft that is close to inflatable ball (34) and be used for monitoring sensor (35) of intestinal and viscera, sensor (35) are connected with watch-dog (11) electricity.

2. The laboratory mouse enteric sensitivity test apparatus of claim 1, wherein: the top surface of the accommodating cavity (21) is provided with an opening (211) and a plurality of guide rods (212) perpendicular to the placing plate (1), a cover plate (23) is arranged above the opening (211), a plurality of guide holes (231) are formed in the cover plate (23), and the guide rods (212) penetrate through the corresponding guide holes (231) to be in threaded connection with an adjusting ring (213).

3. The laboratory mouse enteric sensitivity test apparatus of claim 2, wherein: the bottom surface of apron (23) still is provided with inductor (232) that are used for monitoring laboratory mouse sign.

4. The laboratory mouse enteric sensitivity test apparatus of claim 3, wherein: the both sides of spacing frame (2) are equallyd divide and are provided with two spacing holes (213) that are used for supplying laboratory mouse four limbs to pass to the both sides top surface all is provided with two spacing grooves (214), apron (23) are less than opening (211) size, and two connecting rods (233) with spacing groove (214) looks adaptation of the equal fixedly connected with in both sides, four connecting rod (233) all set up in the top of corresponding spacing groove (214), and be connected with vertical dead lever (234), dead lever (234) set up in the outside of spacing frame (2), and the lower extreme all is connected with the spacing head (235) of U-shaped.

5. The laboratory mouse enteric sensitivity test apparatus of claim 4, wherein: the top surface of apron (23) is provided with spacing seat (24), the top surface of spacing seat (24) is provided with fixed slot (241) and regulation hole (242) to the top is provided with clamp plate (25), the bottom surface of clamp plate (25) is provided with indent (251) and through-hole (252), indent (251) and fixed slot (241) correspond the setting from top to bottom, through-hole (252) threaded connection has regulation pole (253), the lower extreme of adjusting pole (253) passes through-hole (252) to with regulation hole (242) threaded connection.

6. The laboratory mouse enteric sensitivity test apparatus of claim 5, wherein: the number of the adjusting holes (242), the number of the through holes (252) and the number of the adjusting rods (253) are two, the two adjusting holes (242) are respectively arranged on two sides of the fixing groove (241), and the two through holes (252) and the two adjusting rods (253) are respectively arranged on two sides of the pressing groove (251).

7. The laboratory mouse enteric sensitivity test apparatus of claim 6, wherein: the cover plate (23) and the opening (211) are rectangular.

8. The laboratory mouse enteric sensitivity test apparatus according to any one of claims 1 to 7, wherein: still sliding connection has piston (321) in inflator pump (32) to be provided with the scale on the barrel, piston (321) are connected with aerify pole (322), be provided with push pedal (323) on aerifing pole (322), fixedly connected with gag lever post (324) on push pedal (323) the face towards inflator pump (32), the place ahead of gag lever post (324) is provided with sliding sleeve (36), sliding sleeve (36) cover is established on inflator pump (32) to with inflator pump (32) sliding connection, and be provided with locating hole (361), locating hole (361) threaded connection has fastening screw (362), locating hole (361) are passed to the one end of fastening screw (362) for the butt is on inflator pump (32).

9. The laboratory mouse gut sensitivity test apparatus of claim 8, wherein: inflator pump (32) can be dismantled and connect on mount pad (31), place and install electric telescopic handle (12) on board (1), the one end of electric telescopic handle (12) can be dismantled and connect on push pedal (323).

10. The laboratory mouse enteric sensitivity test apparatus according to any one of claims 1 to 7, wherein: install three-way switching valve (331) on gas tube (33), be provided with exhaust hole (332) and electric commutator (333) on three-way switching valve (331), be provided with control center (13) on watch-dog (11), control center (13) are connected with electric commutator (333) electricity.