CN113640512B - 用于多路测定的解码方法及相关流体设备、试剂盒和固体支持物 - Google Patents
用于多路测定的解码方法及相关流体设备、试剂盒和固体支持物 Download PDFInfo
- Publication number
- CN113640512B CN113640512B CN202110961051.0A CN202110961051A CN113640512B CN 113640512 B CN113640512 B CN 113640512B CN 202110961051 A CN202110961051 A CN 202110961051A CN 113640512 B CN113640512 B CN 113640512B
- Authority
- CN
- China
- Prior art keywords
- solid supports
- population
- bead
- encoded
- encoding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Optical Measuring Cells (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562237324P | 2015-10-05 | 2015-10-05 | |
| US62/237,324 | 2015-10-05 | ||
| PCT/US2016/055407 WO2017112025A2 (en) | 2015-10-05 | 2016-10-05 | Decoding methods for multiplexing assays and associated fluidic devices, kits, and solid supports |
| CN201680058434.4A CN108351352B (zh) | 2015-10-05 | 2016-10-05 | 用于多路测定的解码方法及相关流体设备、试剂盒和固体支持物 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201680058434.4A Division CN108351352B (zh) | 2015-10-05 | 2016-10-05 | 用于多路测定的解码方法及相关流体设备、试剂盒和固体支持物 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113640512A CN113640512A (zh) | 2021-11-12 |
| CN113640512B true CN113640512B (zh) | 2024-12-31 |
Family
ID=58609951
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110961051.0A Active CN113640512B (zh) | 2015-10-05 | 2016-10-05 | 用于多路测定的解码方法及相关流体设备、试剂盒和固体支持物 |
| CN201680058434.4A Active CN108351352B (zh) | 2015-10-05 | 2016-10-05 | 用于多路测定的解码方法及相关流体设备、试剂盒和固体支持物 |
| CN202411937836.4A Pending CN119846200A (zh) | 2015-10-05 | 2016-10-05 | 用于多路测定的解码方法及相关流体设备、试剂盒和固体支持物 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201680058434.4A Active CN108351352B (zh) | 2015-10-05 | 2016-10-05 | 用于多路测定的解码方法及相关流体设备、试剂盒和固体支持物 |
| CN202411937836.4A Pending CN119846200A (zh) | 2015-10-05 | 2016-10-05 | 用于多路测定的解码方法及相关流体设备、试剂盒和固体支持物 |
Country Status (5)
| Country | Link |
|---|---|
| US (3) | US11016084B2 (https=) |
| EP (2) | EP3329279B1 (https=) |
| JP (1) | JP6847934B2 (https=) |
| CN (3) | CN113640512B (https=) |
| WO (1) | WO2017112025A2 (https=) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013176767A1 (en) | 2012-05-25 | 2013-11-28 | The University Of North Carolina At Chapel Hill | Microfluidic devices, solid supports for reagents and related methods |
| EP3329279B1 (en) | 2015-10-05 | 2021-07-07 | The University of North Carolina at Chapel Hill | Decoding methods for multiplexing assays and associated fluidic devices, kits, and solid supports |
| US11753681B2 (en) | 2017-11-03 | 2023-09-12 | University Of Washington | Digital nucleic acid amplification using encoded particles |
| EP3794080B1 (en) | 2018-05-18 | 2024-04-10 | The University of North Carolina at Chapel Hill | Compositions, devices, and methods for improving a surface property of a substrate |
| CN119492871A (zh) | 2018-09-26 | 2025-02-21 | 北卡罗来纳-查佩尔山大学 | 用于改进测定的化合物、组合物和方法 |
| JP7550055B2 (ja) | 2018-11-13 | 2024-09-12 | ザ ユニバーシティ オブ ノース カロライナ アット チャペル ヒル | マイクロ流体デバイスを備える分析システムとマイクロ流体デバイスと関連方法 |
| DE102019220017A1 (de) * | 2019-12-18 | 2021-06-24 | Robert Bosch Gesellschaft mit beschränkter Haftung | Aufnahmeeinheit zum Aufnehmen eines Fluids, Verfahren und Vorrichtung zum Herstellen einer Aufnahmeeinheit, Verfahren und Vorrichtung zum Betreiben einer Aufnahmeeinheit und Aufnahmeeinrichtung |
| JP2023550693A (ja) * | 2020-10-27 | 2023-12-05 | クアンタム-エスアイ インコーポレイテッド | 光退色情報を使用する単一分子検出システム |
| WO2022242849A1 (en) * | 2021-05-19 | 2022-11-24 | Leica Microsystems Cms Gmbh | Method and device for analyzing a biological sample |
Family Cites Families (48)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| ES8706823A1 (es) * | 1985-03-28 | 1987-06-16 | Cetus Corp | Un procedimiento para detectar la presencia o ausencia de al menos una secuencia especifica de acidos nucleicos en una muestra |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US6387707B1 (en) | 1996-04-25 | 2002-05-14 | Bioarray Solutions | Array Cytometry |
| US6074827A (en) | 1996-07-30 | 2000-06-13 | Aclara Biosciences, Inc. | Microfluidic method for nucleic acid purification and processing |
| US7622294B2 (en) * | 1997-03-14 | 2009-11-24 | Trustees Of Tufts College | Methods for detecting target analytes and enzymatic reactions |
| US6023540A (en) * | 1997-03-14 | 2000-02-08 | Trustees Of Tufts College | Fiber optic sensor with encoded microspheres |
| US6327410B1 (en) * | 1997-03-14 | 2001-12-04 | The Trustees Of Tufts College | Target analyte sensors utilizing Microspheres |
| US20030027126A1 (en) * | 1997-03-14 | 2003-02-06 | Walt David R. | Methods for detecting target analytes and enzymatic reactions |
| US6143496A (en) | 1997-04-17 | 2000-11-07 | Cytonix Corporation | Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly |
| US7348181B2 (en) | 1997-10-06 | 2008-03-25 | Trustees Of Tufts College | Self-encoding sensor with microspheres |
| US7115884B1 (en) * | 1997-10-06 | 2006-10-03 | Trustees Of Tufts College | Self-encoding fiber optic sensor |
| JP3662850B2 (ja) * | 1998-06-24 | 2005-06-22 | イルミナ インコーポレイテッド | 微小球を有するアレイセンサーのデコード |
| WO2000047996A2 (en) | 1999-02-09 | 2000-08-17 | Illumina, Inc. | Arrays comprising a fiducial and automated information processing in randomly ordered arrays |
| ATE412184T1 (de) * | 1999-08-17 | 2008-11-15 | Luminex Corp | Verfahren zur analyse einer mehrzahl von proben verschiedenen ursprungs auf einen analyten |
| WO2002007729A1 (en) | 2000-07-26 | 2002-01-31 | Taro Pharmaceutical Industries Ltd. | Non-sedating barbiturate compounds as neuroprotective agents |
| WO2002099982A2 (en) * | 2001-03-01 | 2002-12-12 | Illumina, Inc. | Methods for improving signal detection from an array |
| AU2002307152A1 (en) | 2001-04-06 | 2002-10-21 | California Institute Of Technology | Nucleic acid amplification utilizing microfluidic devices |
| AU2002368207A1 (en) * | 2001-10-18 | 2004-05-04 | Virtual Arrays, Inc. | Coded particles for multiplexed analysis of biological samples |
| US8697029B2 (en) * | 2002-04-18 | 2014-04-15 | The Regents Of The University Of Michigan | Modulated physical and chemical sensors |
| AU2004219707B2 (en) * | 2003-03-07 | 2009-04-23 | Rutgers, The State University Of New Jersey | Optically decodable microcarriers, arrays and methods |
| US7148492B2 (en) * | 2003-05-13 | 2006-12-12 | Affymetrix, Inc. | System, method, and product for providing a wavelength tunable excitation beam |
| US20050250141A1 (en) | 2004-03-30 | 2005-11-10 | Lambert James L | Diagnostic assays including multiplexed lateral flow immunoassays with quantum dots |
| JP2008505321A (ja) * | 2004-07-02 | 2008-02-21 | ブルーシフト・バイオテクノロジーズ・インコーポレーテッド | 蛍光体微小環境の探索 |
| EP1963531B1 (en) * | 2005-12-23 | 2011-09-21 | Nanostring Technologies, Inc. | Nanoreporters and methods of manufacturing and use thereof |
| ATE538377T1 (de) * | 2007-04-02 | 2012-01-15 | Acoustic Cytometry Systems Inc | Verfahren zur verbesserten analyse von in einem akustischen feld fokussierten zellen und partikeln |
| EP2167964B1 (de) * | 2007-06-13 | 2015-03-11 | Attomol GmbH Molekulare Diagnostika | Verfahren zur durchführung und auswertung von mix&measure assays für die messung von reaktionskinetiken sowie von konzentrationen und affinitäten von analyten im multiplexformat |
| EP2017354A1 (en) * | 2007-07-20 | 2009-01-21 | Eppendorf Ag | Detection and/or quantification of target molecules on a solid support |
| US8222047B2 (en) | 2008-09-23 | 2012-07-17 | Quanterix Corporation | Ultra-sensitive detection of molecules on single molecule arrays |
| US8236574B2 (en) | 2010-03-01 | 2012-08-07 | Quanterix Corporation | Ultra-sensitive detection of molecules or particles using beads or other capture objects |
| WO2011109379A1 (en) | 2010-03-01 | 2011-09-09 | Quanterix Corporation | Methods and systems for extending dynamic range in assays for the detection of molecules or particles |
| US8824113B2 (en) * | 2010-03-15 | 2014-09-02 | International Truck Intellectual Property Company, Llc | High voltage interlock circuit utilizing serial communications |
| US20120088691A1 (en) * | 2010-10-08 | 2012-04-12 | Gao Chen | Highly multiplexed real-time pcr using encoded microbeads |
| EP4361609A3 (en) * | 2012-02-03 | 2024-07-17 | California Institute of Technology | Signal encoding and decoding in multiplexed biochemical assays |
| WO2013176767A1 (en) | 2012-05-25 | 2013-11-28 | The University Of North Carolina At Chapel Hill | Microfluidic devices, solid supports for reagents and related methods |
| WO2013188927A1 (en) | 2012-06-22 | 2013-12-27 | Macquarie University | Multiplex suspension assay/array using lifetime coding |
| US8921277B2 (en) * | 2012-09-26 | 2014-12-30 | Palo Alto Research Center Incorporated | Multiplexed flow assay based on absorption-encoded micro beads |
| WO2014164739A1 (en) | 2013-03-13 | 2014-10-09 | The University Of North Carolina At Chapel Hill | Nanofluidic devices for the rapid mapping of whole genomes and related systems and methods of analysis |
| JP6506747B2 (ja) | 2013-06-25 | 2019-04-24 | ユニバーシティ オブ ワシントン スルー イッツ センター フォー コマーシャリゼーション | サンプル体積の自己デジタル処理 |
| US20150224499A1 (en) | 2014-02-13 | 2015-08-13 | SFC Fluidics, Inc. | Automated Microfluidic Sample Analyzer Platforms for Point of Care |
| CN114272966B (zh) | 2015-07-22 | 2023-11-07 | 北卡罗来纳-查佩尔山大学 | 含有具有空间分离的珠保留和信号检测段的珠孔几何结构的流体装置和相关方法 |
| CN108027203B (zh) | 2015-07-22 | 2020-11-03 | 北卡罗来纳-查佩尔山大学 | 具有利用冰成核剂的冻结-解冻阀的流体装置和相关的操作和分析方法 |
| EP3329279B1 (en) | 2015-10-05 | 2021-07-07 | The University of North Carolina at Chapel Hill | Decoding methods for multiplexing assays and associated fluidic devices, kits, and solid supports |
| WO2017185098A1 (en) | 2016-04-22 | 2017-10-26 | Purdue Research Foundation | High-throughput particle capture and analysis |
| EP3794080B1 (en) | 2018-05-18 | 2024-04-10 | The University of North Carolina at Chapel Hill | Compositions, devices, and methods for improving a surface property of a substrate |
| CN119492871A (zh) | 2018-09-26 | 2025-02-21 | 北卡罗来纳-查佩尔山大学 | 用于改进测定的化合物、组合物和方法 |
-
2016
- 2016-10-05 EP EP16858484.5A patent/EP3329279B1/en active Active
- 2016-10-05 US US15/765,749 patent/US11016084B2/en active Active
- 2016-10-05 EP EP21183583.0A patent/EP3957996B1/en active Active
- 2016-10-05 WO PCT/US2016/055407 patent/WO2017112025A2/en not_active Ceased
- 2016-10-05 CN CN202110961051.0A patent/CN113640512B/zh active Active
- 2016-10-05 JP JP2018517432A patent/JP6847934B2/ja active Active
- 2016-10-05 CN CN201680058434.4A patent/CN108351352B/zh active Active
- 2016-10-05 CN CN202411937836.4A patent/CN119846200A/zh active Pending
-
2021
- 2021-04-29 US US17/244,257 patent/US12146876B2/en active Active
-
2024
- 2024-10-04 US US18/906,315 patent/US20250027934A1/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| Photobleaching kitnetics-based bead encoding for multiplexed bioassays;Thomas H LINZ 等;Lab on a chip;20170101;第17卷(第6期);1076-1082 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2017112025A2 (en) | 2017-06-29 |
| WO2017112025A3 (en) | 2017-09-08 |
| JP6847934B2 (ja) | 2021-03-24 |
| CN119846200A (zh) | 2025-04-18 |
| EP3329279A2 (en) | 2018-06-06 |
| EP3957996A1 (en) | 2022-02-23 |
| EP3329279B1 (en) | 2021-07-07 |
| CN108351352B (zh) | 2021-09-10 |
| US20250027934A1 (en) | 2025-01-23 |
| US20220011300A1 (en) | 2022-01-13 |
| JP2018538514A (ja) | 2018-12-27 |
| CN113640512A (zh) | 2021-11-12 |
| US11016084B2 (en) | 2021-05-25 |
| US20190094212A1 (en) | 2019-03-28 |
| US12146876B2 (en) | 2024-11-19 |
| CN108351352A (zh) | 2018-07-31 |
| EP3957996B1 (en) | 2025-09-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113640512B (zh) | 用于多路测定的解码方法及相关流体设备、试剂盒和固体支持物 | |
| US20240033739A1 (en) | Fluidic devices with bead well geometries with spatially separated bead retention and signal detection segments and related methods | |
| Rödiger et al. | Nucleic acid detection based on the use of microbeads: a review | |
| Park et al. | Immunomagnetic nanoparticle-based assays for detection of biomarkers | |
| CN112236512B (zh) | 具有微流体装置的分析系统、微流体装置和相关方法 | |
| US20110160090A1 (en) | Nanocrystal-Based Lateral Flow Microarrays and Low-Voltage Signal Detection Systems | |
| US20100167294A1 (en) | Methods for detecting nucleic acids in a sample | |
| WO2017075649A1 (en) | Multiplex lateral flow devices and assays | |
| US10684212B2 (en) | Method and system for reference-assisted droplet detection, indexing and sorting for assays and diagnostics | |
| CN110760571A (zh) | 数字化定量的多靶标共同、生物检测 | |
| JP2018531369A6 (ja) | 空間的に分離してビーズを保持するビーズウェル形状及びシグナル検出セグメントを有する流体デバイス並びに関連する方法 | |
| CN115232707A (zh) | 用于核酸定量的微流体虹吸阵列 | |
| Konry et al. | Particles and microfluidics merged: perspectives of highly sensitive diagnostic detection | |
| Kuo et al. | Digital and absolute assays for low abundance molecular biomarkers | |
| Oita et al. | Microfluidics in macro-biomolecules analysis: macro inside in a nano world | |
| Schulze et al. | Multiplexed optical pathogen detection with lab‐on‐a‐chip devices | |
| Fan et al. | Advances in optical counting and imaging of micro/nano single-entity reactors for biomolecular analysis | |
| Yue et al. | Microfluidics for DNA and protein analysis with multiplex microbead-based assays |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |