CN113621019A - Method for extracting liquorice small-molecule peptide liquid and peptide powder by electromagnetic cracking technology - Google Patents
Method for extracting liquorice small-molecule peptide liquid and peptide powder by electromagnetic cracking technology Download PDFInfo
- Publication number
- CN113621019A CN113621019A CN202110780384.3A CN202110780384A CN113621019A CN 113621019 A CN113621019 A CN 113621019A CN 202110780384 A CN202110780384 A CN 202110780384A CN 113621019 A CN113621019 A CN 113621019A
- Authority
- CN
- China
- Prior art keywords
- liquorice
- licorice
- peptide
- electromagnetic
- small
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 title claims abstract description 114
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 103
- 244000303040 Glycyrrhiza glabra Species 0.000 title claims abstract description 69
- 235000011477 liquorice Nutrition 0.000 title claims abstract description 69
- 238000005336 cracking Methods 0.000 title claims abstract description 57
- 239000007788 liquid Substances 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 36
- 239000000843 powder Substances 0.000 title claims abstract description 36
- 238000005516 engineering process Methods 0.000 title claims description 19
- -1 liquorice small-molecule Chemical class 0.000 title description 4
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 claims abstract description 76
- 241000202807 Glycyrrhiza Species 0.000 claims abstract description 48
- 235000001453 Glycyrrhiza echinata Nutrition 0.000 claims abstract description 45
- 235000017382 Glycyrrhiza lepidota Nutrition 0.000 claims abstract description 45
- 229940010454 licorice Drugs 0.000 claims abstract description 45
- 150000003384 small molecules Chemical class 0.000 claims abstract description 24
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 19
- 229920001184 polypeptide Polymers 0.000 claims abstract description 16
- 239000000126 substance Substances 0.000 claims abstract description 16
- 229920002521 macromolecule Polymers 0.000 claims abstract description 15
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 14
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 14
- 150000001413 amino acids Chemical class 0.000 claims abstract description 11
- 230000003796 beauty Effects 0.000 claims abstract description 10
- 230000005672 electromagnetic field Effects 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000004806 packaging method and process Methods 0.000 claims abstract description 8
- 230000001954 sterilising effect Effects 0.000 claims abstract description 8
- 230000036541 health Effects 0.000 claims abstract description 7
- 239000011343 solid material Substances 0.000 claims abstract description 7
- 230000009471 action Effects 0.000 claims abstract description 5
- 238000004140 cleaning Methods 0.000 claims abstract description 4
- 238000001816 cooling Methods 0.000 claims abstract description 4
- 238000005520 cutting process Methods 0.000 claims abstract description 4
- 238000011049 filling Methods 0.000 claims abstract description 4
- 238000001914 filtration Methods 0.000 claims abstract description 4
- 238000002791 soaking Methods 0.000 claims abstract description 4
- 231100000167 toxic agent Toxicity 0.000 claims abstract description 3
- 239000003440 toxic substance Substances 0.000 claims abstract description 3
- 230000000694 effects Effects 0.000 claims description 18
- 239000008280 blood Substances 0.000 claims description 15
- 210000004369 blood Anatomy 0.000 claims description 15
- 238000001514 detection method Methods 0.000 claims description 11
- 208000030507 AIDS Diseases 0.000 claims description 8
- 206010028980 Neoplasm Diseases 0.000 claims description 7
- 208000005392 Spasm Diseases 0.000 claims description 6
- 239000003814 drug Substances 0.000 claims description 6
- 230000003647 oxidation Effects 0.000 claims description 6
- 238000007254 oxidation reaction Methods 0.000 claims description 6
- 208000011580 syndromic disease Diseases 0.000 claims description 6
- 241000700605 Viruses Species 0.000 claims description 5
- 230000008901 benefit Effects 0.000 claims description 5
- 238000009826 distribution Methods 0.000 claims description 5
- 206010019799 Hepatitis viral Diseases 0.000 claims description 4
- 206010061218 Inflammation Diseases 0.000 claims description 4
- 208000004880 Polyuria Diseases 0.000 claims description 4
- 208000025865 Ulcer Diseases 0.000 claims description 4
- 201000011510 cancer Diseases 0.000 claims description 4
- 230000035619 diuresis Effects 0.000 claims description 4
- 230000004054 inflammatory process Effects 0.000 claims description 4
- 239000002799 interferon inducing agent Substances 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 231100000397 ulcer Toxicity 0.000 claims description 4
- 201000001862 viral hepatitis Diseases 0.000 claims description 4
- 206010011224 Cough Diseases 0.000 claims description 3
- 206010020751 Hypersensitivity Diseases 0.000 claims description 3
- 208000007101 Muscle Cramp Diseases 0.000 claims description 3
- 208000007443 Neurasthenia Diseases 0.000 claims description 3
- 208000001132 Osteoporosis Diseases 0.000 claims description 3
- 208000026935 allergic disease Diseases 0.000 claims description 3
- 230000007815 allergy Effects 0.000 claims description 3
- 206010003549 asthenia Diseases 0.000 claims description 3
- 208000006673 asthma Diseases 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 claims description 3
- 230000036772 blood pressure Effects 0.000 claims description 3
- 239000000470 constituent Substances 0.000 claims description 3
- 210000004211 gastric acid Anatomy 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 239000008236 heating water Substances 0.000 claims description 3
- 239000005556 hormone Substances 0.000 claims description 3
- 229940088597 hormone Drugs 0.000 claims description 3
- 230000036039 immunity Effects 0.000 claims description 3
- 208000030603 inherited susceptibility to asthma Diseases 0.000 claims description 3
- 210000004185 liver Anatomy 0.000 claims description 3
- 210000004072 lung Anatomy 0.000 claims description 3
- 230000004060 metabolic process Effects 0.000 claims description 3
- 235000016709 nutrition Nutrition 0.000 claims description 3
- 230000035764 nutrition Effects 0.000 claims description 3
- 238000010926 purge Methods 0.000 claims description 3
- 210000002460 smooth muscle Anatomy 0.000 claims description 3
- 230000000638 stimulation Effects 0.000 claims description 3
- 201000005060 thrombophlebitis Diseases 0.000 claims description 3
- 230000024883 vasodilation Effects 0.000 claims description 3
- 210000002784 stomach Anatomy 0.000 claims description 2
- 238000012360 testing method Methods 0.000 claims description 2
- 230000008859 change Effects 0.000 claims 1
- 210000000936 intestine Anatomy 0.000 claims 1
- 150000002632 lipids Chemical class 0.000 claims 1
- 239000000654 additive Substances 0.000 abstract description 3
- 230000000996 additive effect Effects 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 239000000047 product Substances 0.000 description 18
- LPLVUJXQOOQHMX-UHFFFAOYSA-N glycyrrhetinic acid glycoside Natural products C1CC(C2C(C3(CCC4(C)CCC(C)(CC4C3=CC2=O)C(O)=O)C)(C)CC2)(C)C2C(C)(C)C1OC1OC(C(O)=O)C(O)C(O)C1OC1OC(C(O)=O)C(O)C(O)C1O LPLVUJXQOOQHMX-UHFFFAOYSA-N 0.000 description 9
- 229960004949 glycyrrhizic acid Drugs 0.000 description 9
- UYRUBYNTXSDKQT-UHFFFAOYSA-N glycyrrhizic acid Natural products CC1(C)C(CCC2(C)C1CCC3(C)C2C(=O)C=C4C5CC(C)(CCC5(C)CCC34C)C(=O)O)OC6OC(C(O)C(O)C6OC7OC(O)C(O)C(O)C7C(=O)O)C(=O)O UYRUBYNTXSDKQT-UHFFFAOYSA-N 0.000 description 9
- 235000019410 glycyrrhizin Nutrition 0.000 description 9
- 230000008569 process Effects 0.000 description 9
- 239000004378 Glycyrrhizin Substances 0.000 description 8
- 241000699670 Mus sp. Species 0.000 description 7
- 230000007062 hydrolysis Effects 0.000 description 7
- 238000006460 hydrolysis reaction Methods 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 238000000605 extraction Methods 0.000 description 5
- MPDGHEJMBKOTSU-YKLVYJNSSA-N 18beta-glycyrrhetic acid Chemical compound C([C@H]1C2=CC(=O)[C@H]34)[C@@](C)(C(O)=O)CC[C@]1(C)CC[C@@]2(C)[C@]4(C)CC[C@@H]1[C@]3(C)CC[C@H](O)C1(C)C MPDGHEJMBKOTSU-YKLVYJNSSA-N 0.000 description 4
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- 235000019658 bitter taste Nutrition 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- GSZUGBAEBARHAW-UHFFFAOYSA-N sophoraflavone B Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C(C=2OC3=CC(O)=CC=C3C(=O)C=2)C=C1 GSZUGBAEBARHAW-UHFFFAOYSA-N 0.000 description 4
- KSDSYIXRWHRPMN-UHFFFAOYSA-N 4'-O-beta-D-Galactopyranoside-6''-p-Coumaroylprunin-4',5,7-Trihydroxyflavanone Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C(C2OC3=CC(O)=CC(O)=C3C(=O)C2)C=C1 KSDSYIXRWHRPMN-UHFFFAOYSA-N 0.000 description 3
- 241000725303 Human immunodeficiency virus Species 0.000 description 3
- DEMKZLAVQYISIA-ONJCETCRSA-N Liquiritin Natural products O([C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1)c1ccc([C@@H]2Oc3c(C(=O)C2)ccc(O)c3)cc1 DEMKZLAVQYISIA-ONJCETCRSA-N 0.000 description 3
- DEMKZLAVQYISIA-UHFFFAOYSA-N Liquirtin Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C(C2OC3=CC(O)=CC=C3C(=O)C2)C=C1 DEMKZLAVQYISIA-UHFFFAOYSA-N 0.000 description 3
- 230000002496 gastric effect Effects 0.000 description 3
- 239000000413 hydrolysate Substances 0.000 description 3
- JBQATDIMBVLPRB-UHFFFAOYSA-N isoliquiritigenin Natural products OC1=CC(O)=CC=C1C1OC2=CC(O)=CC=C2C(=O)C1 JBQATDIMBVLPRB-UHFFFAOYSA-N 0.000 description 3
- DEMKZLAVQYISIA-ZRWXNEIDSA-N liquiritin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C([C@H]2OC3=CC(O)=CC=C3C(=O)C2)C=C1 DEMKZLAVQYISIA-ZRWXNEIDSA-N 0.000 description 3
- 210000003491 skin Anatomy 0.000 description 3
- 235000019640 taste Nutrition 0.000 description 3
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- MPDGHEJMBKOTSU-UHFFFAOYSA-N Glycyrrhetinsaeure Natural products C12C(=O)C=C3C4CC(C)(C(O)=O)CCC4(C)CCC3(C)C1(C)CCC1C2(C)CCC(O)C1(C)C MPDGHEJMBKOTSU-UHFFFAOYSA-N 0.000 description 2
- 229930003268 Vitamin C Natural products 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 229960003720 enoxolone Drugs 0.000 description 2
- 230000007071 enzymatic hydrolysis Effects 0.000 description 2
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 241000411851 herbal medicine Species 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- LTINPJMVDKPJJI-UHFFFAOYSA-N iodinated glycerol Chemical compound CC(I)C1OCC(CO)O1 LTINPJMVDKPJJI-UHFFFAOYSA-N 0.000 description 2
- DXDRHHKMWQZJHT-FPYGCLRLSA-N isoliquiritigenin Chemical compound C1=CC(O)=CC=C1\C=C\C(=O)C1=CC=C(O)C=C1O DXDRHHKMWQZJHT-FPYGCLRLSA-N 0.000 description 2
- 235000008718 isoliquiritigenin Nutrition 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 235000019154 vitamin C Nutrition 0.000 description 2
- 239000011718 vitamin C Substances 0.000 description 2
- 230000037303 wrinkles Effects 0.000 description 2
- CJIJXIFQYOPWTF-UHFFFAOYSA-N 7-hydroxycoumarin Natural products O1C(=O)C=CC2=CC(O)=CC=C21 CJIJXIFQYOPWTF-UHFFFAOYSA-N 0.000 description 1
- 244000205574 Acorus calamus Species 0.000 description 1
- 235000006480 Acorus calamus Nutrition 0.000 description 1
- 108090000145 Bacillolysin Proteins 0.000 description 1
- 108091005658 Basic proteases Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000220485 Fabaceae Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 240000008917 Glycyrrhiza uralensis Species 0.000 description 1
- 235000000554 Glycyrrhiza uralensis Nutrition 0.000 description 1
- 235000018142 Hedysarum alpinum var americanum Nutrition 0.000 description 1
- 240000006461 Hedysarum alpinum var. americanum Species 0.000 description 1
- 241000825107 Hierochloe Species 0.000 description 1
- 235000015466 Hierochloe odorata Nutrition 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- YNWXJFQOCHMPCK-UHFFFAOYSA-N Isoliquiritin Natural products OC1C(O)C(O)C(CO)OC1OC(C=C1)=CC=C1C=CC(=O)C1=CC=C(O)C=C1O YNWXJFQOCHMPCK-UHFFFAOYSA-N 0.000 description 1
- VTAJIXDZFCRWBR-UHFFFAOYSA-N Licoricesaponin B2 Natural products C1C(C2C(C3(CCC4(C)CCC(C)(CC4C3=CC2)C(O)=O)C)(C)CC2)(C)C2C(C)(C)CC1OC1OC(C(O)=O)C(O)C(O)C1OC1OC(C(O)=O)C(O)C(O)C1O VTAJIXDZFCRWBR-UHFFFAOYSA-N 0.000 description 1
- FURUXTVZLHCCNA-UHFFFAOYSA-N Liquiritigenin Natural products C1=CC(O)=CC=C1C1OC2=CC(O)=CC=C2C(=O)C1 FURUXTVZLHCCNA-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- XQWFHGOIUZFQPJ-USTPAGJBSA-N Neoisoliquiritigenin Natural products O=C(/C=C/c1ccc(O)cc1)c1c(O)cc(O[C@H]2[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O2)cc1 XQWFHGOIUZFQPJ-USTPAGJBSA-N 0.000 description 1
- XQWFHGOIUZFQPJ-LXGDFETPSA-N Neoisoliquiritin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC(C=C1O)=CC=C1C(=O)\C=C\C1=CC=C(O)C=C1 XQWFHGOIUZFQPJ-LXGDFETPSA-N 0.000 description 1
- XQWFHGOIUZFQPJ-UHFFFAOYSA-N Neoisoliquiritin Natural products OC1C(O)C(O)C(CO)OC1OC(C=C1O)=CC=C1C(=O)C=CC1=CC=C(O)C=C1 XQWFHGOIUZFQPJ-UHFFFAOYSA-N 0.000 description 1
- HJBUYKZTEBZNSH-ONJCETCRSA-N Neoliquiritin Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(C(=O)C[C@@H](O2)C=3C=CC(O)=CC=3)C2=C1 HJBUYKZTEBZNSH-ONJCETCRSA-N 0.000 description 1
- HJBUYKZTEBZNSH-ZRWXNEIDSA-N Neoliquiritin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(C(=O)C[C@H](O2)C=3C=CC(O)=CC=3)C2=C1 HJBUYKZTEBZNSH-ZRWXNEIDSA-N 0.000 description 1
- 102000035092 Neutral proteases Human genes 0.000 description 1
- 108091005507 Neutral proteases Proteins 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 206010047141 Vasodilatation Diseases 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011033 desalting Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 201000005577 familial hyperlipidemia Diseases 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229960005150 glycerol Drugs 0.000 description 1
- 239000001685 glycyrrhizic acid Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- YNWXJFQOCHMPCK-LXGDFETPSA-N isoliquiritin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC(C=C1)=CC=C1\C=C\C(=O)C1=CC=C(O)C=C1O YNWXJFQOCHMPCK-LXGDFETPSA-N 0.000 description 1
- FURUXTVZLHCCNA-AWEZNQCLSA-N liquiritigenin Chemical compound C1=CC(O)=CC=C1[C@H]1OC2=CC(O)=CC=C2C(=O)C1 FURUXTVZLHCCNA-AWEZNQCLSA-N 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- ORHBXUUXSCNDEV-UHFFFAOYSA-N umbelliferone Chemical compound C1=CC(=O)OC2=CC(O)=CC=C21 ORHBXUUXSCNDEV-UHFFFAOYSA-N 0.000 description 1
- HFTAFOQKODTIJY-UHFFFAOYSA-N umbelliferone Natural products Cc1cc2C=CC(=O)Oc2cc1OCC=CC(C)(C)O HFTAFOQKODTIJY-UHFFFAOYSA-N 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/185—Vegetable proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/011—Hydrolysed proteins; Derivatives thereof from plants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/06—Anti-spasmodics, e.g. drugs for colics, esophagic dyskinesia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
- A61P11/06—Antiasthmatics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
- A61P11/14—Antitussive agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/12—Drugs for genital or sexual disorders; Contraceptives for climacteric disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
- A61P19/10—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease for osteoporosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/06—Antihyperlipidemics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
- A61P31/18—Antivirals for RNA viruses for HIV
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/20—Antivirals for DNA viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/08—Antiallergic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/02—Antidotes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P5/00—Drugs for disorders of the endocrine system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/10—Antioedematous agents; Diuretics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/12—Antihypertensives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/14—Vasoprotectives; Antihaemorrhoidals; Drugs for varicose therapy; Capillary stabilisers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/13—Preparation or pretreatment of starting material involving cleaning, e.g. washing or peeling
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Virology (AREA)
- Diabetes (AREA)
- Pulmonology (AREA)
- Immunology (AREA)
- Oncology (AREA)
- Molecular Biology (AREA)
- Communicable Diseases (AREA)
- Toxicology (AREA)
- Hematology (AREA)
- Endocrinology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Rheumatology (AREA)
- Physical Education & Sports Medicine (AREA)
- Epidemiology (AREA)
- Nutrition Science (AREA)
- Biochemistry (AREA)
- Cardiology (AREA)
- Obesity (AREA)
- Dermatology (AREA)
- Heart & Thoracic Surgery (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Botany (AREA)
- Birds (AREA)
- Neurosurgery (AREA)
- Pain & Pain Management (AREA)
Abstract
The invention discloses a method for extracting liquorice micromolecule peptide liquid and peptide powder by electromagnetic cracking and application thereof, wherein the method comprises the following steps: (1) selecting liquorice, cleaning with clear water, soaking for 8-10 hours, cutting the liquorice into slices with the thickness of 2-4MM after the water content reaches about 40% -50%, putting the sliced liquorice into an electromagnetic cracking device, and (2) electromagnetically cracking protein, various amino acids and macromolecular substances with various components in the liquorice into micromolecular substances under the action of high-frequency alternating electromagnetic field force; (3) cooling and sterilizing the steam in a condenser to obtain licorice micromolecule polypeptide liquid; simultaneously, crushing the dried licorice solid material subjected to electromagnetic cracking to obtain licorice micromolecule polypeptide powder; (4) filtering, filling and packaging the licorice small-molecule polypeptide liquid to obtain licorice small-molecule peptide liquid; sterilizing and packaging the licorice small-molecule polypeptide powder to obtain the licorice small-molecule peptide powder. The product has no additive, no toxic substance, high safety, good water solubility, and easy absorption. Can be widely applied to the fields of beauty treatment, health care, medical treatment and the like.
Description
Technical Field
The invention relates to a method for extracting liquorice micromolecule peptide liquid and peptide powder by an electromagnetic cracking technology; the extraction method is to obtain liquorice micromolecule peptide liquid and peptide powder by using liquorice as a raw material and utilizing an electromagnetic cracking physical technology.
Background
Licorice (academic name:Glycyrrhiza uralensis Fischrubus plants of the genus glycyrrhiza of the family pteroideae are a tonic Chinese herbal medicine. It mostly grows in arid and semiarid desert grasslands, desert edges and loess hilly lands, and is distributed in northwest, north China, northeast and the like.
The licorice belongs to perennial herbs, the root and the rhizome are strong, and the medicinal part is the root and the rhizome. The medicinal material root is cylindrical, the outer skin is not uniform, and the surface is reddish brown or grayish brown. The rhizome is cylindrical, the surface has bud marks, the middle part of the section has marrow, the smell is slight, and the taste is sweet and special.
The licorice is mainly distributed in inner Mongolia, Ningxia, Xinjiang and Gansu; the family Licorice is mainly produced in the Hexi corridor of Gansu, around Longxi, and in Ningxia.
The licorice, also known as Gulao, sweet grass and sweet root, is a perennial herb of Glycyrrhiza of Leguminosae, and the root and rhizome of the licorice are good tonifying Chinese herbal medicines. The Chinese medicine licorice contains several chemical components, including glycyrrhizin, glycyrrhetinic acid, liquiritin, isoliquiritin, neoliquiritin, neoisoliquiritin, liquiritigenin, isoliquiritigenin, liquiritin, glycyl alcohol, isoliquiritigenin, 7-methyl coumarine, umbelliferone, etc. recently, it has been found that licorice possesses the functions of resisting inflammation, resisting ulcer, preventing and curing viral hepatitis and hyperlipemia, resisting cancer, resisting diuresis, resisting interferon inducer and raising cell immunity, and especially has the effect of preventing and curing AIDS. A large number of researches show that glycyrrhizin, glycyrrhizic acid, glycyrrhizin, glycyrrhetinic acid and flavonoids in liquorice are important physiological active substances and mainly exist in the part inside the epidermis of liquorice root.
At present, the production of licorice small molecular peptide products in various countries in the world is an enzymatic hydrolysis process, the chemical method has complex extraction and process procedures, and the bitter taste of hydrolysate or the generation of some harmful chemical solvents cannot be fundamentally solved. The method has the advantages of no additive in the whole preparation process, simple process, easy control, no harmful substance generation in the production process, and guarantee of the purity and safety of the product, thereby having wide popularization and application price.
According to the latest investigation result, the method for producing the liquorice polypeptide at home and abroad is an enzymatic hydrolysis process, and has the following problems:
(1) the enzyme used for hydrolysis is single in variety (only trypsin, alkaline protease, neutral protease and papain are available, the hydrolysis degree is low, and the hydrolysate has poor flavor (bitter taste).
(2) The relationship among the enzymolysis mechanism, the hydrolysis degree and the characteristics of zymolyte is not clear, so that the control of the hydrolysis process lacks theoretical guidance and is difficult to control;
(3) the bitter taste of hydrolysis cannot be solved fundamentally, and the application field is limited;
(4) the enzymolysis process is complex: pretreatment of liquorice, protein separation, enzyme hydrolysis, hydrolysate separation, debittering, desalting and the like, so that the production cost is high;
The above problems are: low hydrolysis degree, poor flavor, poor quality control and high production cost, and the liquorice polypeptide product is difficult to be sold on the market.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provides a method for extracting liquorice micromolecule peptide liquid and peptide powder by an electromagnetic cracking technology, which extracts the liquorice micromolecule peptide liquid and the peptide powder by utilizing an electromagnetic cracking physical technology; the invention not only improves the utilization rate of the liquorice, but also has wide market prospect in the aspects of beauty treatment, health care, medical treatment and the like.
In order to achieve the above object, the present invention adopts the following technical measures.
The method for extracting the liquorice micromolecule peptide liquid and the liquorice peptide powder by the electromagnetic cracking technology comprises the following steps:
(1) pretreatment: cleaning the selected liquorice with clear water, soaking for 8-10 hours to ensure that the water content of the liquorice reaches about 40-50 percent, and then cutting into slices with the thickness of 2-4 MM;
(2) electromagnetic cracking: placing soaked and sliced liquorice in a material hopper of a feeding area of an electromagnetic cracking device, inputting the liquorice into an electromagnetic suppressor through a parallel transmission belt, then entering an electromagnetic cracking box, performing electromagnetic cracking on the liquorice under the action of high-frequency alternating electromagnetic field, gradually heating the electromagnetic cracking box, controlling the temperature to be 100-110 ℃, heating water in the liquorice into steam, diffusing the steam outwards, continuously twisting and stretching macromolecular substance proteins, various amino acids and macromolecular substances of various components in the liquorice under the influence of the alternating electromagnetic field, so that the molecules of the macromolecular substance proteins, various amino acids and the macromolecular substances are cracked into micromolecular substances by electromagnetic cracking, taking out the micromolecular substances along with the steam, performing electromagnetic cracking, cooling steam containing micromolecular liquorice by a condenser, sterilizing, collecting micromolecular polypeptide liquid, and outputting dried liquorice solid materials from a discharge port after the liquorice is subjected to electromagnetic cracking;
(3) Filtering, filling and packaging the licorice small-molecule polypeptide-containing liquid obtained in the step (2) to obtain licorice small-molecule peptide liquid;
(4) and (3) crushing the dried licorice solid material obtained in the step (2), sterilizing and packaging to obtain licorice small molecule peptide powder.
The method of the invention uses an electronic technology and electromagnetically cracks the liquorice under the action of high-frequency alternating electromagnetic field force.
Compared with the prior art, the method has the following advantages and beneficial effects.
The method of the invention has no additive in the whole preparation process, ensures the purity and safety of the product, and has the advantages of easy absorption, good water solubility and the like.
The product of the invention has unique taste and smell, no mould smell, no peculiar smell, no bitter taste, good taste and slight fragrance, the extraction method and the product of the process are superior to the enzymolysis extraction method and the product, and the electromagnetic cracking licorice small molecular peptide liquid and the licorice small molecular peptide powder are products containing peptide segments with small molecular weight and molecular weight distribution about 497 on average through the quality detection of the Chinese medicine of the third party.
The extraction method of the invention can make the protein, various amino acids, microelements, oxidation resistance, etc. in the liquorice fully overflow under the influence of the alternating electromagnetic field, the product takes peptide (especially small molecular peptide) as the constituent substance of the protein, and the product can provide high-quality and easily absorbed 'nutrition' for cells.
The product of the invention can increase the vitality of cells, enhance immunity, resist ulcer, prevent and treat viral hepatitis, resist diuresis, resist interferon inducers, tonify qi and strengthen the middle warmer, relieve spasm and pain, moisten lung and relieve cough, purge fire and remove toxicity, inhibit inflammation, resist allergy, inhibit tumor, resist virus, resist HIV (acquired immune deficiency syndrome virus) activity, reduce blood fat, lower blood sugar, lower blood pressure, prevent and resist cancer, resist oxidation, protect liver and regulate hormone level, improve osteoporosis, climacteric syndrome, resist gastric acid and relieve gastrointestinal smooth muscle spasm, treat neurasthenia, bronchial asthma, thrombophlebitis, promote vasodilation, activate cell activity, effectively eliminate free radicals harmful to human bodies, promote the metabolism of organisms and improve the capability of resisting harmful stimulation.
The method has the advantages of low production cost, simple process, easy operation, high cracking degree and no generation of harmful substances, and can be widely used in the fields of beauty treatment, health care, medical treatment and the like.
Drawings
FIG. 1 is a top view of an electromagnetic cracking apparatus.
FIG. 2 is a plan view of the whole electromagnetic cracking apparatus.
FIG. 3 is a schematic diagram of the process of electromagnetically cracking liquiritin liquid and peptide powder.
Detailed Description
The following detailed description of the embodiments of the present invention is provided, but the present invention is not limited to the following description.
Example 1
The method for extracting the liquorice micromolecule peptide liquid and the liquorice peptide powder by the electromagnetic cracking technology comprises the following steps:
(1) pretreatment: cleaning the selected liquorice with clear water, soaking for 8-10 hours to ensure that the water content of the liquorice reaches about 40-50 percent, and then cutting into slices with the thickness of 2-4 MM;
(2) electromagnetic cracking: placing soaked and sliced liquorice in a material hopper of a feeding area of an electromagnetic cracking device, inputting the liquorice into an electromagnetic suppressor through a parallel transmission belt, then entering an electromagnetic cracking box, performing electromagnetic cracking on the liquorice under the action of high-frequency alternating electromagnetic field, gradually heating the electromagnetic cracking box, controlling the temperature to be 100-110 ℃, heating water in the liquorice into steam, diffusing the steam outwards, continuously twisting and stretching macromolecular substance proteins, various amino acids and macromolecular substances of various components in the liquorice under the influence of the alternating electromagnetic field, so that the molecules of the macromolecular substance proteins, various amino acids and the macromolecular substances are cracked into micromolecular substances by electromagnetic cracking, taking out the micromolecular substances along with the steam, performing electromagnetic cracking, cooling steam containing micromolecular liquorice by a condenser, sterilizing, collecting micromolecular polypeptide liquid, and outputting dried liquorice solid materials from a discharge port after the liquorice is subjected to electromagnetic cracking;
(3) Filtering, filling and packaging the licorice small-molecule polypeptide-containing liquid obtained in the step (2) to obtain licorice small-molecule peptide liquid;
(4) and (3) crushing the dried licorice solid material obtained in the step (2), sterilizing and packaging to obtain licorice small molecule peptide powder.
The components contained in the licorice small-molecule peptide liquid and the licorice small-molecule peptide powder obtained in example 1 are detected, and the data is tested by the national third-party Chinese medicine quality detection (northern) center, Jilin province Ji detection technology Limited company, and the detection basis is as follows: (1) the contents of licorice small-molecule peptide liquid and licorice small-molecule peptide powder extracted by an electromagnetic cracking device, which are detected by the above method, are shown in table 1 in the chinese pharmacopoeia 2015 edition four general rules (0514) and (2) GB 5009.5-2016.
Main nutrient components and content
TABLE 1
As can be seen from Table 1, the invention utilizes the electronic technology to convert proteins, various amino acids and macromolecular substances of various components in licorice into micromolecular various amino acids, vitamins, antioxidant substances and the like after electromagnetic cracking, is a method for extracting by a physical method, and is a product containing peptide segments with small molecular weight and molecular weight distribution about 497 on average by the quality detection of Chinese medicine of the third party. The product uses peptide (especially small molecule peptide) as protein constituent, and it can provide high-quality and easily absorbed 'nutrition' for cell, increase cell activity, enhance immunity, resist ulcer, prevent and treat viral hepatitis, resist diuresis, resist interferon inducer, invigorate qi, strengthen the middle warmer, relieve spasm and pain, moisten lung, relieve cough, purge fire, remove toxic substance, inhibit inflammation, resist allergy, inhibit tumor, resist virus, the health-care food has the effects of resisting HIV (acquired immune deficiency syndrome) activity, reducing blood fat, blood sugar and blood pressure, preventing and resisting cancer, resisting oxidation, protecting liver, regulating hormone level, improving osteoporosis and climacteric syndrome, resisting gastric acid and relieving gastrointestinal smooth muscle spasm, treating neurasthenia, bronchial asthma and thrombophlebitis, promoting vasodilatation, activating cell activity, effectively eliminating free radicals harmful to human bodies, promoting metabolism of organisms and improving the resistance to harmful stimulation.
Example 2
The electromagnetic cracking technology is used for extracting liquorice micromolecule peptide liquid and peptide powder.
The invention aims to extract the beauty treatment effect of the liquorice micromolecule peptide liquid and the peptide powder by electromagnetic cracking; the peptide can be named as the peptide only if the molecular weight section is between 5000 and 50; the polypeptide is called large peptide with the molecular weight section of 5000-10000, small peptide with the molecular weight section of 1000-50 and small molecular active polypeptide, the electromagnetically cracked licorice small molecular peptide liquid and the polypeptide powder are detected by the national third-party Chinese medicine quality detection (northern) center, Jilin province Ji detection technology Limited company, and the electromagnetically cracked licorice small molecular peptide liquid and the polypeptide powder contain the peptide section with the small molecular weight and the molecular weight distribution which are averagely about 497; as is known, many beauty products cannot be directly absorbed by cells, cannot quickly resist oxidation, maintain beauty and keep young, help the body to absorb and fully utilize vitamin C, and also can prevent the cells from being damaged by free radicals, so that wrinkles are reduced, and the skin is shinier; the liquorice micromolecule peptide liquid and peptide powder products obtained by electromagnetic cracking have small molecular weight, so the liquorice micromolecule peptide liquid and peptide powder products have the capability of being directly and completely absorbed by cells, have the effects of quickly resisting oxidation, maintaining beauty and keeping young, helping the body to absorb and fully utilizing vitamin C, and also can prevent the cells from being damaged by free radicals, thereby reducing the generation of wrinkles and ensuring the skin to be glossy; therefore, the liquorice small molecular peptide is added into a plurality of beauty products, so the liquorice small molecular peptide has wide application prospect in beauty industry.
Example 3
The liquorice micromolecule peptide liquid and peptide powder are extracted by an electromagnetic cracking technology, and the health care and medical effects are achieved.
Laboratory animals and groups
Mice were randomized into 4 groups of 60 mice each, and body weights between groups were tested to be not significantly different. A control group, a low dose (0.5 g/kg. d) of electromagnetically cracked licorice small-molecule peptide, a medium dose (1 g/kg. d) of electromagnetically cracked licorice small-molecule peptide and a high dose (2 g/kg. d) of electrically cracked licorice small-molecule peptide, wherein the control group is given physiological saline with the same volume and adopts an intragastric perfusion method to perfuse the stomach with 20 mL/kg. d of gastric perfusion-1Each index was measured once a day for 30 consecutive days.
Experimental methods
(1) According to the technical standards for health food inspection and evaluation and the standards for functional science evaluation procedures and inspection methods of health products issued by Ministry of health, the specified method execution of the hyperlipidemia experiment can be obviously improved.
TABLE 2 Effect of electromagnetically cleaved Glycyrrhiza small-molecule peptides on blood glucose in mice
Table 2 results and analysis:
compared with a control group, the low-dose group of the electromagnetically-cracked licorice small-molecule peptide liquid and the peptide powder can obviously reduce the blood fat of the mouse, and the blood fat reduction rate is 14.8%; the medium and high dose groups of the liquorice micromolecule peptide liquid and the peptide powder are electromagnetically cracked to remarkably reduce the blood fat of mice, and the blood fat reduction rate is respectively 30.2 percent and 51.9 percent; the reduction rate of the blood fat of the mice is obvious dose dependence, so the liquorice micromolecule peptide liquid and the peptide powder which are extracted by electromagnetic cracking can reduce the blood fat of the mice and improve the symptoms of the mice with high blood fat, namely, the liquorice micromolecule peptide liquid and the peptide powder which are extracted by electromagnetic cracking can be judged to have good blood fat reduction effect.
Clinical experiments
The liquorice micromolecule peptide liquid and peptide powder extracted by the electromagnetic cracking technology have good effect on treating AIDS.
The effective component in liquorice for treating AIDS is glycyrrhizin, the glycyrrhizin component in liquorice accounts for 3.63-13.06%, according to test tube experiments, in cells infected with AIDS virus, 0.25 mg of glycyrrhizin per ml, the cells can still survive, but virus-carrying cells without glycyrrhizin almost die, the effect of clinical experiments is also proved, the effect of 6 patients is remarkable when glycyrrhizin is put into 7 AIDS patients in hospital in Tokyo 4 hospitals, the immunocompetence is enhanced, the immunologic function is recovered, the disease state is improved, and the effect of extracting liquorice micromolecule peptide liquid and peptide powder by an electromagnetic cracking technology for treating AIDS can be judged to be good.
Claims (4)
1. A method for preparing licorice micromolecule peptide liquid and peptide powder by electromagnetic cracking physical technology, the method is to change protein, amino acid and macromolecular substance of multiple components in licorice into micromolecule substance after electromagnetic cracking, the product is detected by domestic detection, the product contains peptide segment with small molecular weight and molecular weight distribution about 497 averagely, this data is the test of the third party Chinese medicine quality detection (north) center of entrusting country, Jilin province Ji detection technology Limited company, the detection is according to: (1) according to Chinese pharmacopoeia 2015 edition four parts general rules (0514) and (2) GB 5009.5-2016, the method is used for detecting that licorice small molecule peptide liquid and licorice small molecule peptide powder extracted by an electromagnetic cracking device contain peptide segments with small molecular weight and molecular weight distribution about 497 on average.
2. The method for extracting the liquorice micromolecule peptide liquid and the liquorice peptide powder by the electromagnetic cracking technology comprises the following steps:
(1) pretreatment: cleaning the selected liquorice with clear water, soaking for 8-10 hours to ensure that the water content of the liquorice reaches about 40-50 percent, and then cutting into slices with the thickness of 2-4 MM;
(2) electromagnetic cracking: placing soaked and sliced liquorice in a material hopper of a feeding area of an electromagnetic cracking device, inputting the liquorice into an electromagnetic suppressor through a parallel transmission belt, then entering an electromagnetic cracking box, performing electromagnetic cracking on the liquorice under the action of high-frequency alternating electromagnetic field, gradually heating the electromagnetic cracking box, controlling the temperature to be 100-110 ℃, heating water in the liquorice into steam, diffusing the steam outwards, continuously twisting and stretching macromolecular substance proteins, various amino acids and macromolecular substances of various components in the liquorice under the influence of the alternating electromagnetic field, so that the molecules of the macromolecular substance proteins, various amino acids and the macromolecular substances are cracked into micromolecular substances by electromagnetic cracking, taking out the micromolecular substances along with the steam, performing electromagnetic cracking, cooling steam containing micromolecular liquorice by a condenser, sterilizing, collecting micromolecular polypeptide liquid, and outputting dried liquorice solid materials from a discharge port after the liquorice is subjected to electromagnetic cracking;
(3) filtering, filling and packaging the licorice small-molecule polypeptide-containing liquid obtained in the step (2) to obtain licorice small-molecule peptide liquid;
(4) And (3) crushing the dried licorice solid material obtained in the step (2), sterilizing and packaging to obtain licorice small molecule peptide powder.
3. The method of claim 2, wherein microwave electromagnetic cracking energy is used in the electromagnetic cracking device, the output frequency of the electromagnetic field is 2450MHz +/-25 MHz, and the output power is 250 KW.
4. The method according to claim 2, wherein the prepared peptide liquid and peptide powder can be applied to beauty, health care, medical treatment and the like, and the product takes peptides (especially small molecular peptides) as the constituent substances of proteins, and can provide high-quality and easily-absorbed 'nutrition' for cells; can increase cell activity, enhance immunity, resist ulcer, prevent and treat viral hepatitis, resist diuresis, resist interferon inducer, benefit qi, strengthen the middle warmer, relieve spasm and pain, moisten lung, relieve cough, purge fire, remove toxic substance, inhibit inflammation, prevent allergy, inhibit tumor, resist virus, resist HIV (acquired immune deficiency syndrome virus) activity, reduce blood lipid, lower blood sugar, lower blood pressure, prevent cancer, resist oxidation, protect liver, regulate hormone level, improve osteoporosis, climacteric syndrome, resist gastric acid, relieve smooth muscle spasm of stomach and intestine, treat neurasthenia, bronchial asthma, thrombophlebitis, promote vasodilation, activate cell activity, effectively remove harmful free radicals, promote organism metabolism, and improve resistance to harmful stimulation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110780384.3A CN113621019A (en) | 2021-07-10 | 2021-07-10 | Method for extracting liquorice small-molecule peptide liquid and peptide powder by electromagnetic cracking technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110780384.3A CN113621019A (en) | 2021-07-10 | 2021-07-10 | Method for extracting liquorice small-molecule peptide liquid and peptide powder by electromagnetic cracking technology |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113621019A true CN113621019A (en) | 2021-11-09 |
Family
ID=78379452
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110780384.3A Pending CN113621019A (en) | 2021-07-10 | 2021-07-10 | Method for extracting liquorice small-molecule peptide liquid and peptide powder by electromagnetic cracking technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113621019A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115089694A (en) * | 2022-07-25 | 2022-09-23 | 中国中医科学院中医基础理论研究所 | Liquorice protein extract and new application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2712931Y (en) * | 2004-05-26 | 2005-07-27 | 孙震 | Electromagnetic cleavage device for cleavage extracting polypeptide from vegetable protein |
| CN102613567A (en) * | 2012-04-17 | 2012-08-01 | 孙震 | Soybean peptide nutrient solution and preparation method and application thereof |
| CN104352551A (en) * | 2014-10-29 | 2015-02-18 | 孙震 | Licorice root powder obtained through electromagnetic pyrolysis and preparation method and application of licorice root powder |
| CN111616302A (en) * | 2020-06-03 | 2020-09-04 | 孙震 | Electromagnetically cracked tartary buckwheat germ micromolecule liquid and powder products, and preparation methods and applications thereof |
| CN112961208A (en) * | 2021-03-11 | 2021-06-15 | 吉林省金鹿药业有限公司 | Electromagnetic cracking ginseng small molecule peptide liquid and powder products, and preparation method and application thereof |
-
2021
- 2021-07-10 CN CN202110780384.3A patent/CN113621019A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2712931Y (en) * | 2004-05-26 | 2005-07-27 | 孙震 | Electromagnetic cleavage device for cleavage extracting polypeptide from vegetable protein |
| CN102613567A (en) * | 2012-04-17 | 2012-08-01 | 孙震 | Soybean peptide nutrient solution and preparation method and application thereof |
| CN104352551A (en) * | 2014-10-29 | 2015-02-18 | 孙震 | Licorice root powder obtained through electromagnetic pyrolysis and preparation method and application of licorice root powder |
| CN111616302A (en) * | 2020-06-03 | 2020-09-04 | 孙震 | Electromagnetically cracked tartary buckwheat germ micromolecule liquid and powder products, and preparation methods and applications thereof |
| CN112961208A (en) * | 2021-03-11 | 2021-06-15 | 吉林省金鹿药业有限公司 | Electromagnetic cracking ginseng small molecule peptide liquid and powder products, and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 王仁广: "《一种新型电磁裂解技术在中药大黄、黄芩、柴胡提取中的应用研究》", 《中国优秀硕士学位论文全文数据库（医药卫生科技辑）》, vol. 2020, no. 2, pages 057 - 57 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115089694A (en) * | 2022-07-25 | 2022-09-23 | 中国中医科学院中医基础理论研究所 | Liquorice protein extract and new application thereof |
| CN115089694B (en) * | 2022-07-25 | 2023-08-18 | 中国中医科学院中医基础理论研究所 | Glycyrrhiza protein extract and new application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| TWI782283B (en) | Use of walnut oligopeptide powder | |
| CN104106763A (en) | Application of polygonatum polysaccharide in preparation of drugs for assisting inhibition of colon cancer | |
| CN113621019A (en) | Method for extracting liquorice small-molecule peptide liquid and peptide powder by electromagnetic cracking technology | |
| CN106421208B (en) | Pharmaceutical composition with chemical liver injury resistance function and preparation method thereof | |
| CN114042049B (en) | Preparation method of freeze-dried quick-release preparation of fresh rhizoma polygonati | |
| CN108143833A (en) | A kind of preparation method of licorice piece | |
| CN114947127A (en) | Health food composition for improving immunity of tumor patients and preparation method and application thereof | |
| CN109988795B (en) | Separation and purification process of glycyrrhiza polysaccharide | |
| KR20220020147A (en) | Method for preparing Phellinus linteus mycelia extract or Phellinus linteus mycelia extract powder and composition for enhancing immunity comprising them as an active ingredient | |
| CN112042846A (en) | Sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors and preparation method thereof | |
| CN113134025A (en) | Application of broccoli extract in preventing and treating leukopenia | |
| CN108402459B (en) | Snakehead and black-bone chicken herbal composition and preparation method and application thereof | |
| CN107897622B (en) | Preparation method of peanut skin solid beverage | |
| CN107467654B (en) | Kelp and sea cucumber compound extract and preparation method and application thereof | |
| CN111357984A (en) | Nutritional composition for moistening lung and nourishing kidney | |
| KR101093006B1 (en) | Method for producing functional health food containing bezoar bovis used microbes and the functional health food produced by the same | |
| CN108783443A (en) | A kind of tablet and preparation method thereof for internal organs conditioning | |
| CN117099892B (en) | Anti-fatigue fermented beverage containing bifidobacterium lactis SF-B21 and preparation process thereof | |
| CN107582829A (en) | A kind of functional food of cancer-resisting | |
| CN114949114B (en) | Agilawood-containing traditional Chinese medicine composition for enhancing immunity as well as preparation method, preparation and application thereof | |
| CN114470139B (en) | Traditional Chinese medicine composition for treating liver cancer and preparation method thereof | |
| TWI406669B (en) | Use of a chinese herbal extracts for manufacturing ofmedicament for treating anemia | |
| CN108721520A (en) | It is a kind of to be used to improve oral solution of immunity and preparation method thereof | |
| CN113248562A (en) | Method for extracting soybean micromolecule peptide liquid and peptide powder by electromagnetic cracking technology | |
| CN113501858A (en) | Method for extracting buckwheat micromolecule peptide liquid and peptide powder by electromagnetic cracking technology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20211109 |