CN104106763A - Application of polygonatum polysaccharide in preparation of drugs for assisting inhibition of colon cancer - Google Patents
Application of polygonatum polysaccharide in preparation of drugs for assisting inhibition of colon cancer Download PDFInfo
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- CN104106763A CN104106763A CN201410274076.3A CN201410274076A CN104106763A CN 104106763 A CN104106763 A CN 104106763A CN 201410274076 A CN201410274076 A CN 201410274076A CN 104106763 A CN104106763 A CN 104106763A
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- Prior art keywords
- colon cancer
- solomonseal rhizome
- siberian solomonseal
- rhizome polysaccharide
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Molecular Biology (AREA)
- Materials Engineering (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Sustainable Development (AREA)
- Organic Chemistry (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses an application of polygonatum polysaccharide in the preparation of drugs for assisting the inhibition of colon cancer. The polygonatum polysaccharide is preferably prepared through the following steps: adopting polygonatum sibiricum as the raw material, extracting the polygonatum sibiricum with boiling water, removing the proteins, carrying out dialysis, and finally carrying out an ethanol treatment so as to obtain the polygonatum polysaccharide. The polygonatum polysaccharide can modulate the tumor micro-environment by inhibiting the tumor from generating new vessels, carrying out immunity inhibition, carrying out anti-oxidation, inhibiting the growth of colon cancer fibroblast, and the like. Thus the polygonatum polysaccharide has a very good auxiliary effect on inhibiting the colon tumor.
Description
Technical field
The invention belongs to polysaccharide technical field, be specifically related to a kind of Siberian solomonseal rhizome polysaccharide and there is the application in the auxiliary functional food that suppresses colon cancer effect in preparation.
Background technology
Malignant tumour is the serious disease of current harm humans health, chemotherapy is to be applied to one of main method of malignant tumour whole body therapeutic, but most of anti-tumor medicine thing is in killing tumour cell, also major injury normal cell, the antineoplastic of therefore finding high-efficiency low-toxicity is significant.And research is in recent years found, polysaccharide just has antitumor activity and relatively little to the toxic and side effect of human body significantly, the bioactivity research of polysaccharide has been become to the focus of domestic and international experts and scholars' research at medical domain.In recent years find that some polysaccharide and compound thereof are to various diseases, as immunologic derangement, cancer, diabetes, hypertension, hepatitis, thrombus, pneumonia, virus etc. all have significant curative effect, and the adjusting of the various biological phenomenas of participation cell.A large amount of pharmacology and clinical testing show that polysaccharide compound is good immunomodulator, can increase macrophage and leukocytic phagocytic function, the induction generation meronecrosis factor (TNF) and leukin, thereby improve the immunologic function of human body, and to the almost non-toxic side effect of normal cell.
Sealwort is a kind of conventional Chinese medicine, and sealwort is used as medicine with rhizome.There is boosting qi and nourishing yin, invigorating the spleen, moistening lung, kidney benefiting functions, be used for the treatment of weakness of the spleen and the stomach, fatigue and asthenia, dry food is few, deficiency syndrome of the lung cough caused by dryness, asthenia of essence and blood, the interior heat disease such as quench one's thirst, there is no at present and prepares polysaccharide taking sealwort as raw material, and for the product development of a kind of auxiliary inhibition colon cancer aspect.
Summary of the invention
The object of the present invention is to provide Siberian solomonseal rhizome polysaccharide to there is the application in the auxiliary functional food that suppresses colon cancer effect in preparation.
Wherein said auxiliary inhibition colon cancer effect refers to by suppressing tumor angiogenesis, immunosupress, anti-oxidant and suppress the effects such as colon cancer fibroblastic growth and regulate tumor microenvironment, reaches auxiliary effect that suppresses colon cancer tumour.
As one embodiment of the present invention, Siberian solomonseal rhizome polysaccharide in the present invention preferably prepares by the following method: taking sealwort as raw material, through preparing containing extracting in boiling water, deproteinized, dialysis and Ethanol Treatment operation, described Siberian solomonseal rhizome polysaccharide has the effect that regulates tumor microenvironment.
As one of the present invention preferred embodiment, Siberian solomonseal rhizome polysaccharide of the present invention specifically prepares by the following method: taking sealwort as raw material, after repeatedly extracting with boiling water, merge extract, extract is concentrated to obtain to concentrate, in concentrate, add trichloroacetic acid solution to carry out deproteinized processing, after deproteinized, reactant liquor is neutralized, is pricked a bag dialysis treatment, by reactant liquor in bag filter concentrate, centrifugal treating, get supernatant and add ethanol to carry out alcohol precipitation, dry processing, obtain Siberian solomonseal rhizome polysaccharide.
The present invention is in the preparation process of Siberian solomonseal rhizome polysaccharide, and the design parameter of each operation is preferably as follows:
When extracting in boiling water, extraction time is 1 ~ 5 time, and while extraction, the consumption of boiling water is 5 ~ 20 times of sealwort gross mass at every turn, and each extraction time is 1.5 ~ 5 h.
When extracting in boiling water, the consumption of boiling water is preferably 5 ~ 20 times of sealwort gross mass, and extraction time is preferably 3 times, and while wherein extraction for the first time, the consumption of boiling water is preferably 10 ~ 20 times of raw material sealwort gross mass, extracts 2 ~ 5 h; While extraction for the second time, the consumption of boiling water is preferably 10 ~ 20 times of raw material sealwort gross mass, extracts 1.5 ~ 5h; While extraction for the third time, the consumption of boiling water is preferably 10 ~ 20 times of raw material sealwort gross mass, extracts 1.5 ~ 5 h.
Extract is preferably concentrated into approximately 1/10 of former extract cumulative volume and obtains concentrate.
The quality percentage composition of trichloroacetic acid solution of the present invention is preferably 35%, and the deproteinized processing time is preferably 3 ~ 6 h, and the volume of trichloroacetic acid solution is preferably identical with the volume of concentrate.
When neutralisation treatment, can adopt quality percentage composition is 10% sodium hydrate aqueous solution.
While pricking bag dialysis treatment, preferably adopt the bag filter of molecular cut off 3500Da to carry out dialysis treatment.
Ethanol carries out when alcohol precipitation is processed preferably adopting volumn concentration to be preferably 70 ~ 95% ethanol water.
Concentrate and can adopt multiple concentrated mode of the prior art, wherein with Vacuum Concentration the best.When Vacuum Concentration, temperature is preferably 40 DEG C.
Siberian solomonseal rhizome polysaccharide in the present invention, can be directly oral, also can make said multiple formulation in pharmacy, as capsule-type, tablet, pulvis, granule or oral liquid etc., has auxiliary effect that suppresses colon cancer tumour after eating.
Therefore, the formulation of functional food of the present invention is preferably capsule-type, tablet, pulvis, granule or oral liquid etc.
Compared with prior art, tool of the present invention has the following advantages:
(1) Siberian solomonseal rhizome polysaccharide in the present invention, its purity and active high, can be by suppressing tumor angiogenesis, immunosupress, anti-oxidant and suppress the multipaths such as colon cancer fibroblastic growth and regulate tumor microenvironment, it has good auxiliary inhibition colon cancer function of tumor;
(2) preparation method of the Siberian solomonseal rhizome polysaccharide in the present invention, technique simple and stable is efficient, is applicable to suitability for industrialized production, and cost is low;
(3) Siberian solomonseal rhizome polysaccharide in the present invention, can be for the preparation of having the auxiliary functional food that suppresses colon cancer tumor function.
Brief description of the drawings
Fig. 1 is the activity that in the embodiment of the present invention 4, Siberian solomonseal rhizome polysaccharide can suppress the luciferase with BRE reporter gene of BMP2 induction;
Fig. 2 be in the embodiment of the present invention 5 Siberian solomonseal rhizome polysaccharide can suppress TGF beta induced with SMAD2/3/4 reporter gene activity;
Fig. 3 is that in the embodiment of the present invention 6, Siberian solomonseal rhizome polysaccharide can suppress the activity with the luciferase of NF-к B reporter gene;
Fig. 4 is the activity that in the embodiment of the present invention 7, Siberian solomonseal rhizome polysaccharide can suppress the luciferase with HRE reporter gene of Cocl2 induction;
Fig. 5 is that the embodiment of the present invention 8 Siberian solomonseal rhizome polysaccharides can suppress colon cancer fibroblastic growth.
Detailed description of the invention
Below in conjunction with drawings and Examples, the present invention will be further described, but do not limit in any form the present invention.
The each raw material adopting in following examples, if no special instructions, is commercially available prod.
embodiment 1
Siberian solomonseal rhizome polysaccharide in the present embodiment, prepares by the following method: get sealwort raw material, adopt extracting in boiling water, while extraction for the first time, the consumption of water is 20 times of raw material gross mass, boils 5 h and filters; While extraction for the second time, the consumption of water is 20 times of raw material gross mass, boils 3 h and filters; While extraction for the third time, the consumption of water is 10 times of raw material gross mass, boil 2 h and filter, merging filtrate, Vacuum Concentration is about 1/10 to original volume, lower with.Concentrate is carried out to trichloroacetic acid except albumen processing, add 30% trichloroacetic acid (mass percent) of same volume (lower same), after processing time is 4 h, by 10% NaOH(mass percent) neutralization, lower same, after neutralization, reactant liquor is placed in to bag filter and pricks bag dialysis, when dialysis, the molecular cut off of bag filter is about 3500Da conventionally, lower same, then liquid in bag filter is concentrated, centrifugal, getting supernatant adds 85% ethanol water (volumn concentration) of 3 ~ 4 times of volumes to carry out alcohol precipitation process, taking precipitate is through containing (approximately 40 DEG C of vacuum drying, process to obtain Siberian solomonseal rhizome polysaccharide component down together).
embodiment 2
Siberian solomonseal rhizome polysaccharide in the present embodiment, prepares by the following method: get sealwort raw material, adopt extracting in boiling water, while extraction for the first time, the consumption of water is 15 times of sealwort raw material gross mass, boils 5 h and filters; While extraction for the second time, the consumption of water is 15 times of sealwort raw material gross mass, boils 3 h and filters; While extraction for the third time, the consumption of water is 10 times of sealwort raw material gross mass, boils 3 h and filters, merging filtrate, Vacuum Concentration.Concentrate is carried out to trichloroacetic acid except albumen processing, add 25% trichloroacetic acid (mass percent) of same volume, after processing time is 3 h, by 10% NaOH(mass percent) neutralization, after neutralization, reactant liquor is placed in to bag filter and pricks bag dialysis, then liquid in bag filter is concentrated, centrifugal, get supernatant and add 70% ethanol water (volumn concentration) of 3 ~ 4 times of volumes to carry out alcohol precipitation process, taking precipitate is through obtaining Siberian solomonseal rhizome polysaccharide component containing vacuum drying treatment.
embodiment 3
Siberian solomonseal rhizome polysaccharide in the present embodiment, prepares by the following method: get sealwort raw material, adopt extracting in boiling water, while extraction for the first time, the consumption of water is 10 times of sealwort raw material gross mass, boils 3 h and filters, while extraction for the second time, the consumption of water is 20 times of sealwort raw material gross mass, boils 3 filtration h, while extraction for the third time, the consumption of water is 10 times of sealwort raw material gross mass, boiling 5 h filters, merging filtrate, Vacuum Concentration, concentrate is carried out to trichloroacetic acid except albumen processing, add 15% trichloroacetic acid (mass percent) of same volume, after processing time is 5 h, by 10% NaOH(mass percent) neutralization, after neutralization, reactant liquor is placed in to bag filter and pricks bag dialysis, then liquid in bag filter is concentrated, centrifugal, getting supernatant adds 95% ethanol water (volumn concentration) of 3 ~ 4 times of volumes to carry out alcohol precipitation process, taking precipitate is through obtaining Siberian solomonseal rhizome polysaccharide component containing vacuum drying treatment.
embodiment 4 Siberian solomonseal rhizome polysaccharides can suppress the activity of the luciferase with BRE reporter gene of BMP2 induction
C2C12 cell line is purchased from Chinese Academy of Sciences's cell bank, and C2C12-pGF1-BRE cell line is built by Shanghai Pharmaceutical Inst., Chinese Academy of Sciences's fourth laboratory of chatting about, and is stored in liquid nitrogen.C2C12-pGF1-BRE cell, with containing 10 % Gibco hyclone DMEM medium culture, is placed in 37 DEG C of saturated humidities, contains 5% CO
2insulating box in cultivate, with 1 × 10
4the density of cells/well is inoculated in 96 orifice plates, every hole 100 μ L.After adherent 24 h, suck 60 μ L culture mediums, add sample 50 mL (Siberian solomonseal rhizome polysaccharide in embodiment 1) that configure respective concentration, final concentration is respectively 0.5 mg/mL, 0.1 mg/mL, 0.02 mg/mL, and 10 μ LBMP2 are adjusted to final concentration 200 ng/mL.Establish blank group and the contrast of BMP2 group simultaneously.After administration 16 h, suck culture medium, add 20 μ L Reporter Lysis 1X Buffer, the cell pyrolysis liquid of dissolving 20 μ L are transferred to blank, add 40 μ L luciferase substrates, in 3 minutes, read plate, read RLU value.Adopt SPSS statistical software to carry out statistical procedures.Data all with mean ± standard deviation (.
x±
s) represent, between two groups, relatively adopt independent sample
tinspection, relatively adopts one-way analysis of variance between many groups.
Experimental result as shown in Figure 1, Siberian solomonseal rhizome polysaccharide has the activity of the luciferase with BRE reporter gene that suppresses BMP2 induction under 0.1mg/mL low concentration, and this activity increases and significantly increases with polysaccharide concentration, shows that this polysaccharide has dose dependent aspect this activity.Known cancer new vessels is created in the generation evolution of tumour and plays key effect, and BMP2 signal path plays a crucial role in tumor angiogenesis process.This polysaccharide can significantly suppress BMP2 signal path, shows that this polysaccharide has potential adjusting tumor microenvironment effect.
embodiment 5 Siberian solomonseal rhizome polysaccharides can suppress TGF beta induced with SMAD2/3/4 reporter gene activity
HEK293T cell line is purchased from Chinese Academy of Sciences's cell bank, and HEK293T-pGF1-SMAD2/3/4 cell line is built by Shanghai Pharmaceutical Inst., Chinese Academy of Sciences's fourth laboratory of chatting about, and is stored in liquid nitrogen.HEK293T-pGF1-SMAD2/3/4 cell, with containing 10% Gibco hyclone DMEM medium culture, is placed in 37 DEG C of saturated humidities, contains 5% CO
2insulating box in cultivate, with 2 × 10
4the density of cells/well is inoculated in 96 orifice plates, every hole 100 μ L.After adherent 24 h, suck 60 μ L culture mediums, add sample 50 mL (Siberian solomonseal rhizome polysaccharide in embodiment 1) that configure respective concentration, final concentration is respectively 0.5 mg/mL, 0.1 mg/mL, 0.02 mg/mL, and 10 μ LTGF β 1 are adjusted to final concentration 50 ng/mL.Establish blank group and 1 group of contrast of TGF β simultaneously.After administration 16hr, suck culture medium, add 20 μ L Reporter Lysis 1X Buffer, the cell pyrolysis liquid of dissolving 20 μ L are transferred to blank, add 40 μ L luciferase substrates, in 3 minutes, read plate, read RLU value.Adopt SPSS statistical software to carry out statistical procedures.Data all with mean ± standard deviation (.
x±
s) represent, between two groups, relatively adopt independent sample
tinspection, relatively adopts one-way analysis of variance between many groups.
Experimental result as shown in Figure 2, Siberian solomonseal rhizome polysaccharide under 0.5 mg/mL low concentration, have suppress TGF beta induced with SMAD2/3/4 signal path, and this activity increases and significantly increases with Siberian solomonseal rhizome polysaccharide concentration, shows that this polysaccharide has dose dependent aspect this activity.Known cancer new vessels is created in the generation evolution of tumour and plays key effect, and the beta induced SMAD2/3/4 signal path of TGF plays a crucial role in tumor angiogenesis process.This polysaccharide can significantly suppress this signal path, shows that this polysaccharide has potential adjusting tumor microenvironment effect.
embodiment 6 Siberian solomonseal rhizome polysaccharides can suppress the activity with the luciferase of NF-к B reporter gene
THP-1 cell line is purchased from Chinese Academy of Sciences's cell bank, and THP-1/pGF1-NF-к B cell line is built by Shanghai Pharmaceutical Inst., Chinese Academy of Sciences's fourth laboratory of chatting about, and is stored in liquid nitrogen.THP-1/pGF1-NF-к B cell is with containing 10% hyclone RPMI-1640 medium culture.Be placed in 37 DEG C of saturated humidities, contain 5% CO
2insulating box in cultivate, with 5 × 10
4the density of cells/well is inoculated in 96 orifice plates, every hole 50 μ L volumes.Add the Siberian solomonseal rhizome polysaccharide in given the test agent 50 μ L(embodiment 1), final concentration is respectively 0.5 mg/mL, 0. L mg/mL, 0.02 mg/mL, sets up blank group and LPS control group simultaneously.Add LPS solution 10 μ L, final concentration is 1 μ g/mL, is placed in 37 DEG C of saturated humidities, contains 5% CO
2insulating box in overnight incubation.After overnight incubation, every sky adds 100 μ L Bright-Glo
tMluciferase Assay System substrate, reads plate in ELIASA, reads RLU value.
Adopt SPSS statistical software to carry out statistical procedures.Data all with mean ± standard deviation (.
x±
s) represent, between two groups, relatively adopt independent sample
tinspection, relatively adopts one-way analysis of variance between many groups.
As shown in Figure 3, this polysaccharide has the activity of good inhibition NF-к B signal path to experimental result under 0.5 mg/mL low concentration.Known NF-к B is the key nuclear factor that participates in series of genes expression regulation, in its origin in cancer, development, angiogenesis and transfer, has important function, and closely related with the generation of cancer drug resistance.NF-к B participates in these processes by different approach after activating, and the effect of blocking-up NF-к B can suppress the generation of cancer.Therefore, this polysaccharide can pass through to suppress NF-к B signal path, thereby brings into play potential inhibition tumor promotion.
embodiment 7 Siberian solomonseal rhizome polysaccharides can suppress Cocl
2
the activity of the luciferase with HRE reporter gene of induction
HEK293T cell line is purchased from Chinese Academy of Sciences's cell bank, and HEK293T-pGF1-HRE cell line is built by Shanghai medicine institute of the Chinese Academy of Sciences fourth laboratory of chatting about, and is stored in liquid nitrogen, with containing 10% Gibco hyclone DMEM medium culture.HEK293T-pGF1-HRE cell is with 2 × 10
4the density of cells/well is inoculated in 96 orifice plates, every hole 100 μ L.After adherent 24 h, suck 60 μ L culture mediums, add sample (Siberian solomonseal rhizome polysaccharide in embodiment 1) the 50 μ L that configure respective concentration, final concentration is respectively 0.5mg/mL, 0.1mg/mL, 0.02 mg/mL, 10 μ L Cocl
2be adjusted to final concentration 250 μ M.Establish blank group and Cocl simultaneously
2group contrast.After administration 16 h, suck culture medium, add 20 μ L Reporter Lysis 1X Buffer, frozen to reading plate in-80 DEG C of refrigerators.Shift out drafting board to be checked from-80 DEG C of refrigerators, the cell pyrolysis liquid of dissolving 20 μ L are transferred to blank, add 40 μ Lluciferase substrates, in 3 minutes, read plate, read RLU value.Adopt SPSS statistical software to carry out statistical procedures.Data all with mean ± standard deviation (.
x±
s) represent, between two groups, relatively adopt independent sample
tinspection, relatively adopts one-way analysis of variance between many groups.
As shown in Figure 4, Siberian solomonseal rhizome polysaccharide may suppress Cocl to Siberian solomonseal rhizome polysaccharide determination of activity result under the concentration of 0.5 mg/mL
2the activity of the luciferase with HRE reporter gene of induction, proves that it has the antioxidation activity of potential quality.
embodiment 8 Siberian solomonseal rhizome polysaccharides can suppress colon cancer fibroblastic growth
Colon cancer associated fibroblast clone CT26-CAF is built by Institute of Biophysics, Academia Sinica.Cell is inoculated in to 96 orifice plates, and cell density is 1 × 10
4/ hole.Siberian solomonseal rhizome polysaccharide in embodiment 1 is dissolved in DMEM+ /+culture medium, and making its concentration is 1.0 mg/mL.Cultivate 4 days, adopt CCK detection kit (Beijing Zhuan Meng biotech firm) to measure cell growth state.Every kind of polysaccharide sample size is 3.
Statistical analysis: relatively adopt variance analysis between many groups of continuous datas, first data are carried out to homogeneity test of variance, calculate Levene statistical value, if variance is neat, between organizing,, method is LSD and Dunnett to multiple ratio, if heterogeneity of variance, adopts bearing calibration Dunnett t.Statistical software is SPSS17.0, and significance is α=0.05.
As shown in Figure 5, this Siberian solomonseal rhizome polysaccharide can significantly suppress the growth of colon cancer CT26-CAF to experimental result under the concentration of 0.5 mg/mL.CT26-CAF plays important regulating and controlling effect in colon cancer, can promote the growth of colon cancer cell.This polysaccharide can, by suppressing the growth of colon cancer CT26-CAF, play the effect that regulates colon cancer microenvironment.
To sum up experimental results show that Siberian solomonseal rhizome polysaccharide in the present invention can be by suppressing tumor angiogenesis, immunosupress, anti-oxidant and suppress the multipaths such as colon cancer fibroblastic growth and regulate tumor microenvironment, and further bring into play potential inhibitor against colon carcinoma cells tumor promotion, thereby Siberian solomonseal rhizome polysaccharide can be passed through to common process, make said multiple formulation in pharmacy, for the preparation of having the auxiliary functional food that suppresses colon cancer effect, the formulation of functional food can be capsule-type, tablet, pulvis, granule or oral liquid etc.
Above-described embodiment is preferably embodiment of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under Spirit Essence of the present invention and principle, substitutes, combination, simplify; all should be equivalent substitute mode, be included in protection scope of the present invention.
Claims (9)
1. Siberian solomonseal rhizome polysaccharide has the application in the auxiliary functional food that suppresses colon cancer effect in preparation.
2. Siberian solomonseal rhizome polysaccharide according to claim 1 has the application in the auxiliary functional food that suppresses colon cancer effect in preparation, it is characterized in that: described auxiliary inhibition colon cancer effect refers to by suppressing colon cancer tumor angiogenesis, immunosupress, anti-oxidant and inhibition colon cancer fibroblastic growth effect, regulate colon cancer tumor microenvironment, thereby play the auxiliary colon cancer effect that suppresses.
3. Siberian solomonseal rhizome polysaccharide according to claim 1 has the application in the auxiliary functional food that suppresses colon cancer effect in preparation, it is characterized in that: described Siberian solomonseal rhizome polysaccharide prepares by the following method: taking sealwort as raw material, through preparing containing extracting in boiling water, deproteinized, dialysis and Ethanol Treatment operation, described Siberian solomonseal rhizome polysaccharide has the effect that regulates tumor microenvironment.
4. Siberian solomonseal rhizome polysaccharide according to claim 3 has the application in the auxiliary functional food that suppresses colon cancer effect in preparation, it is characterized in that: described Siberian solomonseal rhizome polysaccharide specifically prepares by the following method: taking sealwort as raw material, after repeatedly extracting with boiling water, merge extract, extract is concentrated to obtain to concentrate, in concentrate, add trichloroacetic acid solution to carry out deproteinized processing, after deproteinized, reactant liquor is neutralized, prick bag dialysis treatment, reactant liquor in bag filter is concentrated, centrifugal treating, getting supernatant adds ethanol to carry out alcohol precipitation, dry processing, obtain Siberian solomonseal rhizome polysaccharide.
5. Siberian solomonseal rhizome polysaccharide according to claim 4 has the application in the auxiliary functional food that suppresses colon cancer effect in preparation, it is characterized in that: when extracting in boiling water, extraction time is 1 ~ 5 time, when each extraction, the consumption of boiling water is 5 ~ 20 times of sealwort gross mass, and each extraction time is 1.5 ~ 5 h.
6. Siberian solomonseal rhizome polysaccharide according to claim 4 has the application in the auxiliary functional food that suppresses colon cancer effect in preparation, it is characterized in that: when deproteinized, adopt trichloroacetic acid solution, the quality percentage composition of described trichloroacetic acid solution is 35%, and the processing time is 3 ~ 6 h.
7. Siberian solomonseal rhizome polysaccharide according to claim 4 has the application in the auxiliary functional food that suppresses colon cancer effect in preparation, it is characterized in that: when Ethanol Treatment, adopting volumn concentration is 70 ~ 95% ethanol water.
8. Siberian solomonseal rhizome polysaccharide according to claim 4 has the application in the auxiliary functional food that suppresses colon cancer effect in preparation, it is characterized in that: when dialysis, the molecular cut off of bag filter is 3500Da.
9. Siberian solomonseal rhizome polysaccharide according to claim 1 has the application in the auxiliary functional food that suppresses colon cancer effect in preparation, it is characterized in that: the formulation of described functional food is capsule-type, tablet, pulvis, granule or oral liquid.
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