A kind of soya-bean polypeptides nutrient solution
Technical field
The present invention relates to a kind of health food, be specifically related to a kind of soya-bean polypeptides nutrient solution.
Background technology
Protein is the material base of life, is the human body indispensable nutriment that grows, and protein is made up of several amino acids, wherein is called oligopeptides by 3~10 amino acid with the compound that peptide bond links to each other; Be called polypeptide by 10~50 amino acid with the compound that peptide bond links to each other; 50 are called protein with upper amino acid with the compound that peptide bond links to each other.
After always people think that protein is taken in, must it be hydrolyzed into amino acid whose form through the various enzymes of alimentary canal and just can be absorbed.In recent years, scientist's great deal of research results shows: protein not is that complete hydrolysis becomes amino acid after taking in, but major part just is absorbed when the form of peptide.Find that simultaneously the absorbed speed of polypeptide is faster than free amino acid, absorptivity is higher, particularly little molecule oligopeptides.
Protein is as nutriment; Main form and part free amino acid with peptide could absorb in human body; Bring into play various physiological functions, thus, polypeptide is very close with healthy relation; He reconciles and is arranging life from being born to the overall process of death, and replenishing nutrient protein to human body is the best with the form of polypeptide.Different crowds is replenished the allogenic polypeptide goods in right amount, to human body prevent and cure diseases, raise immunity, it is crucial improving body health quality.
The protein of necessary for human mainly is to absorb in driven, the vegetable protein food.Recent studies shows that animal protein contains great amount of fat, cholesterol, the much more edible generation that can cause many " rich man's diseases " such as hypertension, high fat of blood, coronary heart disease.Vegetable protein substitutes animal protein and is paid attention to by people.
Vegetable protein is main with cereal, and soy protein content is up to more than 40% in the cereal, and the amino acid kind of forming soybean protein is more complete.Wherein, contain the amino acid that 9 kinds of human bodies can not synthesize voluntarily, be called " necessary amino acid ", amino acid balance is good.Soya-bean polypeptides is recommended as " the ideal source of phytoprotein " by FAO (Food and Agriculture Organization of the United Nation).
Research to soy bean protein hydrolysate starts from the sixties in 19th century, is to use acid, alkali under fixed temperature catalytic pyrolysis at that time.But this chemical method causes loss of nutritional ingredients excessive, and side reaction is many, many defectives such as organoleptic properties difference.To the eighties in 19th century; Because the speed of enzymology technology hails exhibition; The U.S., Japan have obtained bigger progress with enzymatic process, to the eighties in 20th century, and Japanology more comprehensive; Carrying is not physicochemical characteristic, different physiological roles and the application system comparatively all to soya-bean polypeptides, and developed multiple functional food, resistance should living infant food, nutritious supplementary pharmaceutical such as motion food.
At present, it all is enzyme hydrolysis process that countries in the world produce soya-bean polypeptides, this method, complex technical process, and the bitter taste of hydrolysate still can not solve at all.Exist many drawbacks such as degree of hydrolysis is low, weak flavor (bitter taste), complex technical process, production cost height, limited the demand in application and market, anxious to be solved is: seek new method, new technology.
China is the native place of soybean, before more than 2000 years, and founder of the Han Dynasty's grandson. Huainan king Liu An; In process is made pills of immortality in " Mt. Bagong " in Jiangsu; Invented with soybean and processed " bean curd ", spread all over all over the world so far, China only is in the preliminary working stage of bean product.Up to the later stage eighties in 20th century, soya-bean polypeptides is just carried out primary study, and list the exploitation and the application of " soya-bean polypeptides " in the enforcement period of the ninth five-year plan national key scientific and technological project.China National Academy of Food & Fermentation Industries is the earliest to the research institution of soya-bean polypeptides, and has born " 95 " brainstorm project.Accomplish soybean or dregs of beans at present and extracted the task of separating soya-bean polypeptides.Used enzymolysis process and soya-bean polypeptides product have all reached world level, maintain the leading position at home, but drop into large-scale production not yet, and product is not still gone public.
According to the latest survey result, the method for producing soya-bean polypeptides both at home and abroad all is an enzyme hydrolysis process, all has following problem:
(1) being used for the enzyme kind of hydrolysis, more single (several kinds in trypsase, alkali protease, neutral proteinase, papain is only arranged, and degree of hydrolysis is low, hydrolysate weak flavor (bitter taste is arranged).
(2) enzymolysis mechanism, degree of hydrolysis and zymolyte characteristic relation are indeterminate, cause the hydrolysis technology controlling and process to lack theoretical direction, and control is difficult.
(3) bitter taste of hydrolysis still can not solve at all, and application is restricted.
(4) the enzymolysis process process is complicated: soybean, and---------hydrolysate separation---debitterize flavor---desalination etc. cause production cost high to protein isolate in enzyme hydrolysis.
Above problem: degree of hydrolysis is low, weak flavor, poor quality control, production cost are high, make the soya-bean polypeptides product be difficult to appear on the market.
Summary of the invention
The objective of the invention is to: a kind of soya-bean polypeptides nutrient solution is provided, multiple disease is had remarkable prevention effect.
Above-mentioned purpose of the present invention realizes through following technical scheme:
A kind of soya-bean polypeptides nutrient solution is provided, and it is to extract the light yellow clarifying liquid body from soybean through physical method, and described method for distilling may further comprise the steps:
1) electromagnetism cracking: soybean is got in the airtight high-frequency and high-voltage alternating electromagnetic field continuously, add thermal material to 100~110 ℃, collect the steam and the condensation that produce, obtain liquid I and solid material I;
2) squeezing lixiviate: solid material I oil meal after the routine oil expression is handled that step 1) is obtained separates, and the dregs of beans that obtains adds the water logging bubble of 3 times of weight, and under ultrasonic vibration, stirs after 30 minutes and carry out Separation of Solid and Liquid, obtains solid material II and liquid II;
3) the liquid II standing demix that filtering and concentrating: with step 2) obtains obtains the supernatant on upper strata and the muddy liquid of lower floor, and supernatant through the molecular sieve filtration of molecular weight below 2000, is leached liquid III; It is concentrated to heat after liquid through molecular sieve and muddy liquid do not mix, and collects the steam and the condensation that produce, percent by weight of dry matter content >=30% in mixed liquor, congee shape solid material III and liquid IV after obtaining concentrating;
4) solid material III that solid material II that dry extracting: with step 2) obtains and step 3) obtain is dried respectively, drops to below 10% until water content wherein, collects the steam that produces and condensation therebetween and mixes and obtain liquid V;
5) merge extract: the liquid V that the liquid I that step 1) is obtained, the liquid III that step 3) obtains and liquid IV and step 4) obtain mixes.
Step 1) can be accomplished through existing various electromagnetism cracking apparatus, and preferably adopting the patent No. is that the electromagnetism cracker of putting down in writing in the utility model patent specification of ZL200420059838 is accomplished.This device comprises totally enclosed silo, material import and export, electromagnetic generator, spiral release strand cage assembly and condenser; Described material inlet is a bucket shape, is arranged at the top of silo, and material outlet is a tubular, is arranged at the below of silo, spiral is installed in it releases the strand cage assembly, and this spiral is released the strand cage assembly and driven by gearbox and buncher; Said electromagnetic generator comprises that high-frequency signal the source takes place, is fixed in the coniform electromagnetism anode of central authorities in the silo and forms as three parts of electromagnetism negative electrode with silo; Said condenser is connected with silo inside, and an axial flow blower is set at the condensator outlet place; The output frequency that the source takes place said high-frequency signal is 200KHZ to 2000MHZ, and power output is in 100W to 100KW scope.Get into the cracking storehouse of silo formation when material after; By the effect of high frequency alternating electromagnetic field, material is warming up to 100~110 ℃, and the molecule in the material is under the alternating electromagnetic field influence; Constantly reverse, stretch, make its molecular breakdown; Moisture in the material is lowered the temperature by condenser after being heated to form steam, in the water that collect at the condenser place, contains multiple micromolecule polypeptide composition.
Step 2) the preferred screw oil expeller that adopts of described oil expression processing is accomplished; Described Separation of Solid and Liquid preferably adopts disk centrifuge to accomplish.
The mixed liquor that step 5) obtains is preferably further handled through ultraviolet disinfection.
The present invention also provides the preparation method of described soya-bean polypeptides nutrient solution, may further comprise the steps:
1) electromagnetism cracking: soybean is got in the airtight high-frequency and high-voltage alternating electromagnetic field continuously, add thermal material to 100~110 ℃, collect the steam and the condensation that produce, obtain liquid I and solid material I;
2) squeezing lixiviate: solid material I oil meal after the routine oil expression is handled that step 1) is obtained separates, and the dregs of beans that obtains adds the water logging bubble of 3 times of weight, and under ultrasonic vibration, stirs after 30 minutes and carry out Separation of Solid and Liquid, obtains solid material II and liquid II;
3) the liquid II standing demix that filtering and concentrating: with step 2) obtains obtains the supernatant on upper strata and the muddy liquid of lower floor, and supernatant through the molecular sieve filtration of molecular weight below 2000, is leached liquid III; It is concentrated to heat after liquid through molecular sieve and muddy liquid do not mix, and collects the steam and the condensation that produce, percent by weight of dry matter content >=30% in mixed liquor, congee shape solid material III and liquid IV after obtaining concentrating;
4) solid material III that solid material II that dry extracting: with step 2) obtains and step 3) obtain is dried respectively, drops to below 10% until water content wherein, collects the steam that produces and condensation therebetween and mixes and obtain liquid V;
5) merge extract: the liquid V that the liquid I that step 1) is obtained, the liquid III that step 3) obtains and liquid IV and step 4) obtain mixes.
It is that the electromagnetism cracker of putting down in writing in the utility model patent specification of ZL200420059838 is accomplished that step 1) preferably adopts the patent No..
Step 2) the preferred screw oil expeller that adopts of described oil expression processing is accomplished; Described Separation of Solid and Liquid preferably adopts disk centrifuge to accomplish.
The mixed liquor that step 5) obtains is preferably further handled through ultraviolet disinfection.
The present invention also provides the application of said soya-bean polypeptides nutrient solution in the health food of preparation control chronic hepatitis B, acne, gastric ulcer or superficial gastritis.
Soya-bean polypeptides nutrient solution of the present invention has following beneficial effect:
1. soya-bean polypeptides nutrient solution active component of the present invention is abundant; According to detecting; The soya-bean polypeptides nutrient solution of the embodiment of the invention 1 preparation contains 23 peptide species (molecular weight is below 1000Dalton, and the chromatography result sees Fig. 1) and 18 kinds of free amino acid (9 kinds of necessary amino acid are wherein arranged) and 6 kinds of vitamins; Still contain 4 kinds of activity of fighting against senium materials: reduced form Agifutol (GSH), glutathione-S transferase (GSH-S), catalase (CAT), superoxide dismutase (SOD), the nutrient composition content mean value of concrete sample are seen table 1-3:
Table 1. amino acid kind and content
Table 2. vitamin kind and content
Table 3. antioxidant content and content
Antioxidase |
Content |
Reduced glutathione (GSH) |
110μg/ml |
TAC: |
5.57U/ml |
Glutathione-S transferase (GSH-S) |
192.00U/ml |
Catalase (CAT) |
7.390×10-3U/ml |
Superoxide dismutase (SOD) |
0.27μg/ml |
2. soya-bean polypeptides nutrient solution features good taste of the present invention, does not have stink, has a little fragrance no bitter taste.
3. production method of the present invention is pure physical method, so no any additive in the whole process of preparation, has guaranteed the pure property and the security of product.
4. production cost of the present invention is low, and technology is simple, easy to operate, severity is high.
Nutrient solution of the present invention is because preparation method's cost is low, thereby the market price is relatively low, is prone to accepted by the consumer, and application is wide, and the adaptation population is many, and DEVELOPMENT PROSPECT is good.
Description of drawings
Fig. 1 is the photo that comprises polypeptide chromatography figure in the soya-bean polypeptides nutrient solution of embodiment 1 preparation and data thereof.
The specific embodiment
Embodiment 1. preparation embodiment
A kind of soya-bean polypeptides nutrient solution, method for distilling may further comprise the steps:
1) soybean is got in the electromagnetism cracker described in the ZL200420059838 utility model patent specification specific embodiment continuously, add thermal material to 100~110 ℃, collect the steam and the condensation that produce, obtain liquid I and solid material I;
2) the solid material I that step 1) is obtained adopts screw oil expeller oil meal after the routine oil expression is handled to separate; The dregs of beans that obtains adds the water logging bubble of 3 times of weight; And under ultrasonic vibration, stir and adopt disk centrifuge to carry out Separation of Solid and Liquid after 30 minutes, obtain solid material II and liquid II;
3) with step 2) the liquid II standing demix that obtains, obtain the supernatant on upper strata and the muddy liquid of lower floor, supernatant through the molecular sieve filtration of molecular weight below 2000, is leached liquid III; It is concentrated to heat after liquid through molecular sieve and muddy liquid do not mix, and collects the steam and the condensation that produce, percent by weight of dry matter content >=30% in mixed liquor, congee shape solid material III and liquid IV after obtaining concentrating;
4) with step 2) the solid material III that obtains of the solid material II that obtains and step 3) dried respectively, drop to below 10% until water content wherein, collect the steam that produces and condensation therebetween and mix and obtain liquid V;
5) the liquid V that the liquid I that step 1) is obtained, the liquid III that step 3) obtains and liquid IV and step 4) obtain mixes, and obtains soya-bean polypeptides nutrient solution of the present invention.
The effect clinical report
Soya-bean polypeptides nutrient solution of the present invention has remarkable prevention effect to multiple disease, and concrete effect is seen following experiment:
1 soya-bean polypeptides nutrient solution of the present invention is to the clinical observation on the therapeutic effect of chronic hepatitis B
[observational technique]:
Diagnostic method: " new Chinese medicine clinical research guideline (1993) " diagnostic criteria of developing and publishing according to Ministry of Health of the People's Republic of China.
Group technology: be divided into 2 groups at random, every group 30 people.Control group administration amino acid (animal blood) capsule, each 3, every day 3 times; The soya-bean polypeptides nutrient solution of the treatment group administration embodiment of the invention 1 preparation, each oral 20ml, every day 3 times.
The course of treatment: 1-2 month is a course of treatment;
Methods of treatment: symptom of each comprehensive review, sign, the full item of liver function, immunoglobulin (Ig), blood, routine urinalysis, various viral antigen health check-up survey, liver and gall spleen B ultrasonic before and after the treatment;
Efficacy determination: " new Chinese medicine clinical research guideline (1993) " curative effect determinate standard of developing and publishing according to Ministry of Health of the People's Republic of China.
The basic healing: subjective symptoms disappears, and hepatosplenomegaly is stablized constant or dwindled, no tenderness and kowtow pain, and liver function test is normal, and the virus detection is turned out cloudy, and above each item index was stablized more than 1 year.
Produce effects: subjective symptoms disappears, and hepatosplenomegaly is stablized constant, and does not have obvious tenderness and kowtow pain, and liver function each item is obviously recovered or be normal, and more than lasting half a year.
Effectively: cardinal symptom disappears or basic the disappearance, and hepatosplenomegaly is stablized and do not had obvious tenderness and kowtow pain, and liver function each item checks that positive regular meeting initial value descends more than 50%, and continues three months.
Invalid: after finishing the course of treatment, each item index no change or deterioration.
[observed result]:
Treatment is organized soya-bean polypeptides nutrient solution 1-2 that 30 routine patients take the embodiment of the invention 1 after each course of treatment, and clinical cure 5 examples account for 16.6%, and produce effects 8 examples account for 26.7%, and effective 14 examples account for 46.7%, and invalid 3 examples account for 10%.Total effective rate is 90%, sees table 4 for details.
Table 4
Can find out that from table 1 the soya-bean polypeptides nutrient solution of the embodiment of the invention 1 has excellent curative to chronic hepatitis B.
Table 5
Can find out that from table 5 treatment group surface antigen negative conversion rate can reach 30.4%, e antigen negative conversion rate can reach 27.8%, significantly is superior to control group.
2 soya-bean polypeptides nutrient solutions of the present invention are to the remedy of acne effect observation
The 29 routine patients of dept. of dermatology have been carried out nine months clinical observation, outside for oral administration adding, wiped and use, found recovery from illness 16 examples, accounted for 55.17%, 10 examples that take a turn for the better account for 34.48%, and invalid 3 examples account for 10.39%.
Research thinks that several amino acids, the vitamins composition in the soya-bean polypeptides nutrient solution of the present invention has the effect of regulating nutrition to skin metabolism, and soybean includes flavonoids simultaneously, has the endocrine function of adjusting.3 soya-bean polypeptides nutrient solutions of the present invention are to the clinical observation on the therapeutic effect of gastric ulcer
[diagnostic criteria of gastric ulcer]: by the clinical guidance principle of the nineteen ninety-five new Chinese medicine treatment gastric ulcer developed and published of the Ministry of Public Health.
Western medicine diagnose standard: with reference to the standard of the whole nation, nineteen eighty-two Chongqing gastritis diagnosis and treatment forum formulation;
Differential diagnosis in tcm: with reference to the diagnosis tentative standard of disease of digestive system Professional Committee of CAIM in 1989.
Curative effect determinate standard: according to the clinical guidance principle regulation of the nineteen ninety-five new Chinese medicine treatment gastric ulcer developed and published of the Ministry of Public Health:
1. clinical cure: the clinical symptoms sign disappears, and gastroscope check mucous membrane chronic inflammation takes a turn for the better, and intestinesization are given birth to and recovered normal or disappearance with dysplasia;
2. produce effects: clinical cardinal symptom, sign disappear, and gastrocopy mucous membrane chronic inflammation takes a turn for the better, and intestinesization are given birth to dysplasia and recovered normal or alleviate 2 to spend;
3. effective: the cardinal symptom sign obviously alleviates, and gastroscope check mucosa infection scope is dwindled more than 1/2, and inflammation alleviates to some extent, and ulcer surface reduces; Intestinesization are given birth to and dysplasia alleviates;
4. invalid: as not reach effective standard or anti-the deterioration.
[methods of treatment]:
The soya-bean polypeptides nutrient solution of the treatment group administration embodiment of the invention 1, every day 2 times, each 20ml, one month is a course of treatment, individual month of the heavy 2-3 continuously of the state of an illness, viewing duration all strengthening spleen and nourishing stomach medicines of stopping using.Respectively check OGD and each item physico-chemical examination before and after the treatment, the result is following:
The total effective rate of table 6 soya-bean polypeptides nutrient solution treatment of the present invention gastric ulcer
Divide into groups |
Total routine number |
Clinical cure |
Produce effects |
Effectively |
Invalid |
Total effective rate |
The treatment group |
35 |
3 |
10 |
16 |
6 |
82.8% |
Table 6 embodies soya-bean polypeptides nutrient solution of the present invention can reach 82.8% to the treatment total effective rate of gastric ulcer, and in addition, the inspection of each item symptom and sign also embodies significant improvement.
4 soya-bean polypeptides nutrient solutions of the present invention are to the clinical observation on the therapeutic effect of chronic superficial gastritis
Diagnostic criteria and curative effect determinate standard are all formulated by " new Chinese medicine treatment chronic superficial gastritis clinical research guideline " that the Ministry of Public Health develops and publishes.
[diagnostic criteria]: course of disease delay, in various degree indigestion, apocleisis, the nauseating and relevant symptoms such as upper abdomen pain of taking food are arranged, the left upper abdomen mild tenderness can be arranged;
[curative effect determinate standard]
1. produce effects: symptom and sign all improves more than 2 grades, gastroscope and pathologic finding, and inflammation obviously alleviates;
2. effective: symptom and sign all improves more than 1, gastroscope and pathologic finding, and inflammation alleviates;
3. invalid: the not obvious variation of symptom and sign does not reach effective standard or deterioration
[methods of treatment]:
The former treatment stomach trouble medication dosing kind of taking of 60 routine experimenters is constant, is divided into the therapeutic combination control group at random, and every group 30 example, treatment group add the soya-bean polypeptides nutrient solution of the embodiment of the invention 1 preparation, every day 2 times, each 30ml; Control group does not add any health products or medicine.Took continuously 30 days.Respectively check symptom, sign, OGD and each item physical and chemical index one time before and after the test, the result is following:
The total effective rate of table 7 soya-bean polypeptides nutrient solution treatment of the present invention chronic superficial gastritis
Table 7 can be known; Soya-bean polypeptides nutrient solution of the present invention is significantly higher than the control group of not taking this nutrient solution to the treatment total effective rate of chronic superficial gastritis, in addition, finds in the variation of gastric mucosa through gastric endoscopic examination; The improvement rate of treatment group can reach 35.7%, and control group has only 20.0%; Other symptom and signs also have clear improvement than control group.
In sum, soya-bean polypeptides nutrient solution of the present invention all has excellent curative to chronic hepatitis B, acne, gastric ulcer and chronic superficial gastritis.