CN113614514A - 6-磷酸葡萄糖脱氢酶突变体及其在制备检测试剂中的用途 - Google Patents

6-磷酸葡萄糖脱氢酶突变体及其在制备检测试剂中的用途 Download PDF

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CN113614514A
CN113614514A CN202080024000.9A CN202080024000A CN113614514A CN 113614514 A CN113614514 A CN 113614514A CN 202080024000 A CN202080024000 A CN 202080024000A CN 113614514 A CN113614514 A CN 113614514A
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hapten
glucose
buffer
phosphate dehydrogenase
conjugate
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CN113614514B (zh
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龚俊
肖兰萍
祁金祥
张启飞
王贵利
刘希
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Beijing Strong Biotechnologies Inc
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Abstract

6‑磷酸葡萄糖脱氢酶突变体及其在制备检测试剂中的用途。6‑磷酸葡萄糖脱氢酶突变体相较于野生型6‑磷酸葡萄糖脱氢酶包含以下突变的组合：56C、306C和454C。使用6‑磷酸葡萄糖脱氢酶突变体所制备的检测试剂盒，特异性强、灵敏度高、操作方便、检测时间短、定量准确，适合高通量检测。

Description

PCT国内申请，说明书已公开。

Claims (11)

  1. PCT国内申请，权利要求书已公开。
CN202080024000.9A 2020-01-07 2020-11-04 6-磷酸葡萄糖脱氢酶突变体及其在制备检测试剂中的用途 Active CN113614514B (zh)

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PCT/CN2020/126375 WO2021139375A1 (zh) 2020-01-07 2020-11-04 6-磷酸葡萄糖脱氢酶突变体及其在制备检测试剂中的用途

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CN114113264B (zh) * 2021-12-11 2023-07-07 郑州大学 基于exoⅲ辅助链循环和cha反应的妥布霉素双放大检测方法和应用
CN115655848B (zh) * 2022-12-26 2023-03-17 河北盛华尔生物医疗科技有限公司 一种稳定型的谷草转氨酶测定试剂盒

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6455288B1 (en) * 1993-04-08 2002-09-24 Dade Behring Marburg Gmbh Homogeneous immunoassays using mutant glucose-6-phosphate dehydrogenases
EP2770064A1 (de) * 2013-02-22 2014-08-27 F. Hoffmann-La Roche AG Hocheffiziente Herstellung von Blutglucose-Teststreifen
CN106190996A (zh) * 2016-08-30 2016-12-07 宁波美康生物科技股份有限公司 一种葡萄糖6磷酸脱氢酶突变体
WO2017220486A2 (en) * 2016-06-20 2017-12-28 Pharmazell Gmbh Coupled, self-sufficient biotransformation of chenodeoxycholic acid to ursodeoxycholic acid and novel enzyme mutants applicable in said process
CN110174363A (zh) * 2019-01-09 2019-08-27 北京九强生物技术股份有限公司 6-磷酸葡萄糖脱氢酶突变体及其在制备检测试剂中的用途

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7604969B2 (en) * 2005-06-20 2009-10-20 Arkray, Inc. Mutant glucose dehydrogenase
FR2948680B1 (fr) * 2009-07-28 2013-10-04 Centre Nat Rech Scient Nouveaux mutants de la pqq s-gdh
EP3339431A1 (en) * 2016-12-22 2018-06-27 Roche Diabetes Care GmbH Glucose dehydrogenase variants with improved properties
CN108508194A (zh) * 2018-05-23 2018-09-07 太原瑞盛生物科技有限公司 一种妥布霉素免疫检测试剂及其制备和检测方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6455288B1 (en) * 1993-04-08 2002-09-24 Dade Behring Marburg Gmbh Homogeneous immunoassays using mutant glucose-6-phosphate dehydrogenases
EP2770064A1 (de) * 2013-02-22 2014-08-27 F. Hoffmann-La Roche AG Hocheffiziente Herstellung von Blutglucose-Teststreifen
WO2017220486A2 (en) * 2016-06-20 2017-12-28 Pharmazell Gmbh Coupled, self-sufficient biotransformation of chenodeoxycholic acid to ursodeoxycholic acid and novel enzyme mutants applicable in said process
CN106190996A (zh) * 2016-08-30 2016-12-07 宁波美康生物科技股份有限公司 一种葡萄糖6磷酸脱氢酶突变体
CN110174363A (zh) * 2019-01-09 2019-08-27 北京九强生物技术股份有限公司 6-磷酸葡萄糖脱氢酶突变体及其在制备检测试剂中的用途

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
QI, JINXIANG 等: "Glucose-6-phosphate dehydrogenase mutant D306C has a higher inhibition rate for enzyme multiplied immunoassay of cholyglycine", 《BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY》 *

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CN113614514B (zh) 2022-11-25
CN115856278B (zh) 2024-06-04
WO2021139375A1 (zh) 2021-07-15
JP2023509037A (ja) 2023-03-06
CN115856278A (zh) 2023-03-28
US20230243819A1 (en) 2023-08-03

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