CN1136082A - 微量元素的生物转化及酶化提取方法 - Google Patents
微量元素的生物转化及酶化提取方法 Download PDFInfo
- Publication number
- CN1136082A CN1136082A CN 95105605 CN95105605A CN1136082A CN 1136082 A CN1136082 A CN 1136082A CN 95105605 CN95105605 CN 95105605 CN 95105605 A CN95105605 A CN 95105605A CN 1136082 A CN1136082 A CN 1136082A
- Authority
- CN
- China
- Prior art keywords
- trace elements
- trace element
- enzyme
- amount
- extraction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000011573 trace mineral Substances 0.000 title claims abstract description 24
- 235000013619 trace mineral Nutrition 0.000 title claims abstract description 24
- 238000000605 extraction Methods 0.000 title claims description 10
- 238000006243 chemical reaction Methods 0.000 title claims description 6
- 108090000790 Enzymes Proteins 0.000 claims abstract description 10
- 102000004190 Enzymes Human genes 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 8
- 230000002255 enzymatic effect Effects 0.000 claims description 9
- 229940088598 enzyme Drugs 0.000 claims description 9
- 235000016709 nutrition Nutrition 0.000 claims description 7
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- 108010059892 Cellulase Proteins 0.000 claims description 4
- 108091005804 Peptidases Proteins 0.000 claims description 4
- 102000035195 Peptidases Human genes 0.000 claims description 4
- 229940106157 cellulase Drugs 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 239000004382 Amylase Substances 0.000 claims description 3
- 108010065511 Amylases Proteins 0.000 claims description 3
- 102000013142 Amylases Human genes 0.000 claims description 3
- 244000068988 Glycine max Species 0.000 claims description 3
- 235000010469 Glycine max Nutrition 0.000 claims description 3
- 241000209140 Triticum Species 0.000 claims description 3
- 235000021307 Triticum Nutrition 0.000 claims description 3
- 235000019418 amylase Nutrition 0.000 claims description 3
- 230000036983 biotransformation Effects 0.000 claims description 3
- 229940036811 bone meal Drugs 0.000 claims description 3
- 239000002374 bone meal Substances 0.000 claims description 3
- 229910052602 gypsum Inorganic materials 0.000 claims description 3
- 239000010440 gypsum Substances 0.000 claims description 3
- 239000010903 husk Substances 0.000 claims description 3
- 241000609240 Ambelania acida Species 0.000 claims description 2
- 239000010905 bagasse Substances 0.000 claims description 2
- 229920001277 pectin Polymers 0.000 claims description 2
- 239000001814 pectin Substances 0.000 claims description 2
- 235000010987 pectin Nutrition 0.000 claims description 2
- 238000000855 fermentation Methods 0.000 abstract description 4
- 230000004151 fermentation Effects 0.000 abstract description 4
- 235000015097 nutrients Nutrition 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 2
- 230000009466 transformation Effects 0.000 abstract description 2
- 241000233866 Fungi Species 0.000 description 8
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 7
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 6
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 241000283986 Lepus Species 0.000 description 4
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 4
- 229910052804 chromium Inorganic materials 0.000 description 4
- 239000011651 chromium Substances 0.000 description 4
- 230000004069 differentiation Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000011669 selenium Substances 0.000 description 4
- 229910052711 selenium Inorganic materials 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 108010059820 Polygalacturonase Proteins 0.000 description 3
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 229910052742 iron Inorganic materials 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 239000011701 zinc Substances 0.000 description 3
- 229910052725 zinc Inorganic materials 0.000 description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 244000060234 Gmelina philippensis Species 0.000 description 2
- 241000577951 Hydnum Species 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 2
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 2
- 229910017052 cobalt Inorganic materials 0.000 description 2
- 239000010941 cobalt Substances 0.000 description 2
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 235000015872 dietary supplement Nutrition 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 2
- 229910052750 molybdenum Inorganic materials 0.000 description 2
- 239000011733 molybdenum Substances 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- -1 sulfuric acid ester polysaccharide Chemical class 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 description 1
- 241000222336 Ganoderma Species 0.000 description 1
- 240000000588 Hericium erinaceus Species 0.000 description 1
- 235000007328 Hericium erinaceus Nutrition 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 229920002230 Pectic acid Polymers 0.000 description 1
- 235000007685 Pleurotus columbinus Nutrition 0.000 description 1
- 240000001462 Pleurotus ostreatus Species 0.000 description 1
- 235000001603 Pleurotus ostreatus Nutrition 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 108010019077 beta-Amylase Proteins 0.000 description 1
- 239000003124 biologic agent Substances 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 244000309464 bull Species 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 230000000536 complexating effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 238000009928 pasteurization Methods 0.000 description 1
- LCLHHZYHLXDRQG-ZNKJPWOQSA-N pectic acid Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)O[C@H](C(O)=O)[C@@H]1OC1[C@H](O)[C@@H](O)[C@@H](OC2[C@@H]([C@@H](O)[C@@H](O)[C@H](O2)C(O)=O)O)[C@@H](C(O)=O)O1 LCLHHZYHLXDRQG-ZNKJPWOQSA-N 0.000 description 1
- 239000010318 polygalacturonic acid Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 229940065287 selenium compound Drugs 0.000 description 1
- 150000003343 selenium compounds Chemical class 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
微量元素的生物转化及酶化提取方法，属于真菌学与酶工程学交叉结合的生物方法。其特征在于将适量的微量元素添加到一种超营养基中，经过菌丝体的生物转化，再经系列酶发酵，提取出有机元素及营养物。可制成系列有机微量元素生物药剂及保健品，具有保健和医疗的双重功能。
Description
本发明涉及在一种真菌超营养培养基添加微量元素，利用真菌菌丝体对微量元素的富集、转化能力，将无机态元素转化为有机态元素。对已成熟的含有机态微量元素的菌丝体培养物采用加酶的方法、强制催化发酵、提取出有机微量元素及多种营养成份。为真菌菌丝体在一种超营养培基中转化微量元素及酶法提取提供了一项新的工艺。
微量元素是维持人体健康必不可少的物质，用于做营养补剂和医疗药物，有机态元素较无机态元素更有利于人体的吸收和具有很高的安全性。占人体重量0.05％左右的41种元素，称微量元素，其中包括生命结构元素(钠、钾、钙、镁……)和生命必需微量元素(铁、锌、铜、锰、钴、钼、铬、硒……)。
中科院生态环境中心合成了Kappa-硒化卡拉胶，是一种人工合成的硒与硫酸脂多糖的有机硒化合物。我国王力发明了一种含有多种有机微量元素水的制备方法(公布号：CN1065794A.申请号92102191.7、卷期号8-45)。《食用菌生产大全》指出：不少菌类的子实体和菌丝体都具有相似的成份和疗效。真菌子实体对多种微量元素具有富集、转化能力，已逐渐为国内外学者认识。
本发明的目的在于：利用一种含有植物、动物多种营养成份的超营养培养基，对多种微量元素进行真菌菌丝体转化、富集，再用酶法提取。使之成为含有机元素相当于真菌子实体数十、数百倍，且各种营养成份高度平衡、协调，然后制成系列化含有机微量元素的生物药剂及营养保健品。
真菌菌丝体对微量元素的富集、转化，首先涉及到使用什么培养基。常用的培养基，总是因为杂带有毒成份，口感不适，营养素缺乏等原因而不适于应用到本发明中。发明人经反复试验成功了一种超营养培养基，其配方是：大豆10％、玉米25％、小麦40％、骨粉5％、谷壳(或甘蔗渣、玉米蕊等)19％、石膏1％、水适量。此培养基的特点是由农产品及动物附产物构成，无毒、无怪味、营养丰富、平衡、来源广泛。香菇、平菇、金针菇、猴头、灵芝等食用、药用菌丝体均能适应，生长快速、茂密、粗壮。经不同梯度试验，大部份微量元素(锌、硒、铁、铜、镁、钴、锰、钼、铬……)在此培养基中的添加量为300～500PPM。由于其碳氮比超出了真菌子实体发育的正常碳氮比(1∶20左右)，故上述培养基一般不能正常生长出子实体。在培养基菌丝体长满瓶或袋，紧接着原基开始分化时，是提取其有效成份的最佳时期。
在对添加微量元素的菌丝体培养物进行酶解过程中，必须兼顾酶的活力、底物浓度、PH、时间、温度及催化发酶的设备条件等。本发明一般使用纤维素酶、果胶酶、淀粉酶、蛋白酶，这些酶均属水解酶，可将菌丝体培养物中坚固的纤维素，高度脂化的果胶及果胶酸、淀粉、蛋白等逐级降解，生成低分子化合物。发酵采用间断方式，每一种酶发酵完成，离心沉降过滤后，滤渣中再添加另一种酶。使用酶的顺序是纤维酶→果胶酶→淀粉酶→蛋白酶。酶解后的有机溶液，50％左右的微量元素有机转化或络合，且含有丰富的氨基酸，SOD、单糖、核酸及核酸降解物、微生素。
本发明同现有技术相比所具有的优点在于：现有有机微量元素来自于天然动、植物和人工合成，而本发明则是将真菌学与酶工程学交叉结合，对多种微量元素进行工厂化的生物转化。其产出品中有机态微量元素的含量相当于真菌子实体中的数十、数百倍，且有丰富的有机物与有机微量元素平衡，协调地发挥保健与医疗的双重功能。其材料物广价低，工艺简捷，适宜于工业化生产。
实施例：
例1.猴头菌丝体转化铬及酶法提取。
一、菌丝体培养：将含大豆10％、玉米25％、小麦40％、谷壳19％、骨粉5％、石膏1％、铬500PPM、水120％的配料装瓶、灭菌、接种猴头原种。培养温度23℃～25℃，时间18～22天，待菌丝长满瓶时，在光照150勒克斯、温度为25℃～27℃的条件下培养7天，形成原基分化。
二、酶法提取：菌丝体培养物破碎，按照纤维酶→果胶酶→α-淀粉酶、β-淀粉酶→蛋白酶的顺序，进行酶解。
三、酶发酵液过滤：60℃温度真空减压浓缩。乙醇沉淀过滤。乙醇回收。浓缩液装瓶，巴氏灭菌。
例2.金针菇菌丝体转化硒及酶法提取。
方法同例1。培养基接种对象为金针菇原种，原基分化温度为20℃～22℃。
例3.平菇菌丝体转化锌及酶法提取。
方法同例1。培养基接种对象为平菇原种，原基分化温度为22℃～24℃。
例4.黑木耳菌丝体转化铁及酶法提取。
方法同例1。培养基接种对象为黑木耳。
Claims (3)
1、微量元素的生物转化及酶化提取方法，其特征是在一种超营养培养基中添加适量微量元素，经真菌菌丝体的转化，并用酶法提取。所说的适量的微量元素是指相当于培养基干物量的300～500PPM。
2、根据权利要求1所述的超营养培养基的配方是：大豆10％、玉米25％、小麦40％、骨粉5％、谷壳(或甘蔗渣、玉米蕊等)19％、石膏1％、水适量。
3、根据权利要求1所述酶法提取，其特征是用酶的种类和顺序：纤维素酶→果胶→淀粉酶→蛋白酶。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95105605 CN1136082A (zh) | 1995-05-17 | 1995-05-17 | 微量元素的生物转化及酶化提取方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95105605 CN1136082A (zh) | 1995-05-17 | 1995-05-17 | 微量元素的生物转化及酶化提取方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1136082A true CN1136082A (zh) | 1996-11-20 |
Family
ID=5075544
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 95105605 Pending CN1136082A (zh) | 1995-05-17 | 1995-05-17 | 微量元素的生物转化及酶化提取方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1136082A (zh) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106511402A (zh) * | 2015-09-09 | 2017-03-22 | 李全才 | 一种三氧化二砷的生物转化方法 |
-
1995
- 1995-05-17 CN CN 95105605 patent/CN1136082A/zh active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106511402A (zh) * | 2015-09-09 | 2017-03-22 | 李全才 | 一种三氧化二砷的生物转化方法 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101372671B (zh) | 一种富锌蛹虫草子实体的人工培养方法及其培养基 | |
| CN107488598B (zh) | 一种牛蒡基蛹虫草菌丝体及其制备方法 | |
| CN102285826B (zh) | 红托竹荪液体发酵用液体培养基 | |
| CN109207544A (zh) | 一种小球藻抗氧化多肽的制备方法 | |
| CN1331908A (zh) | 一种中草药食用菌培养基及用其生产食用菌的方法 | |
| CN115039636B (zh) | 一种栽培银耳的培养基及其制作方法 | |
| CN107360858A (zh) | 一种香菇食用菌的培育方法 | |
| CN107012101A (zh) | 一种去皮牛蒡液体发酵培养基及发酵茶新菇的方法 | |
| CN108718915A (zh) | 提高侧耳属食用菌产量的培养基及栽培方法 | |
| CN1031307A (zh) | 供食品加工的食用菌菌丝体快速固体培养法 | |
| KR101027964B1 (ko) | 약용버섯으로 배양된 고아미 쌀을 포함하는 기능성 식품의 제조 방법 | |
| CN1083408C (zh) | 一种多元氨基酸液肥的快速发酵制备方法 | |
| CN1978468A (zh) | 一种富硒灵芝多糖及其制备方法 | |
| CN101032211A (zh) | 一种高营养苹果的培育方法 | |
| CN110279110A (zh) | 一种植物酵素及其制备方法 | |
| CN109006182A (zh) | 一种用香菇菌渣栽培平菇的培养基料及其制备方法 | |
| KR100496666B1 (ko) | 고등균류를 이용한 발효 인삼 제조 방법 및 이를 포함하는기능성 인삼제품 | |
| CN1136082A (zh) | 微量元素的生物转化及酶化提取方法 | |
| CN1303905C (zh) | 一种微生物发酵块茎类农副产品残渣生产膳食纤维的方法 | |
| CN113383840A (zh) | 一种富硒藤茶 | |
| KR20030028638A (ko) | 버섯 균사체 배양에 의한 기능성 뽕잎 건강식품의제조방법 | |
| KR100204984B1 (ko) | 상황버섯 조성물의 배양방법 | |
| CN109328865B (zh) | 一种蛹虫草的制备方法及其制备的蛹虫草 | |
| CN113273434A (zh) | 一种玉米浆源促长剂及其制备方法和应用 | |
| CN111789252A (zh) | 一种酶解发酵法提高玛卡补肾壮阳功效的方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| C53 | Correction of patent of invention or patent application | ||
| CB02 | Change of applicant information |
Address after: Xing Ma Xiang 635612 Sichuan province Nanjiang County Applicant after: Li Quancai Address before: 550002 No. 73, view waterway, Guiyang, Guizhou Applicant before: Cheng Zhongli Applicant before: Li Quancai |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: APPLICANT; FROM: CHENG ZHONGLI; LI QUANCAI TO: LI QUANCAI |
|
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent of invention or patent application | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: APPLICANT; FROM: LI QUANCAI TO: CHONGQING RAINWATER INDUSTRY CO., LTD. |
|
| CP03 | Change of name, title or address |
Address after: 400030 No. 66 Han Yu Road, Chongqing, Shapingba Applicant after: Chongqing rainwater Industry Co.,Ltd. Applicant before: Li Quancai |
|
| C53 | Correction of patent of invention or patent application | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: APPLICANT; FROM: CHONGQING RAINWATER INDUSTRY CO., LTD. TO: LI QUANCAI |
|
| CP03 | Change of name, title or address |
Address after: Wen Xing Road 635600 Sichuan province Nanjiang town No. 98 Applicant after: Li Quancai Address before: 400030 No. 66 Han Yu Road, Chongqing, Shapingba Applicant before: Chongqing rainwater Industry Co.,Ltd. |
|
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |