CN113599298B - Composition for skin antibiosis and repair, preparation method and application thereof - Google Patents
Composition for skin antibiosis and repair, preparation method and application thereof Download PDFInfo
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- CN113599298B CN113599298B CN202111004257.0A CN202111004257A CN113599298B CN 113599298 B CN113599298 B CN 113599298B CN 202111004257 A CN202111004257 A CN 202111004257A CN 113599298 B CN113599298 B CN 113599298B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/27—Zinc; Compounds thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/365—Hydroxycarboxylic acids; Ketocarboxylic acids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/494—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom
- A61K8/4946—Imidazoles or their condensed derivatives, e.g. benzimidazoles
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- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/606—Nucleosides; Nucleotides; Nucleic acids
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/731—Cellulose; Quaternized cellulose derivatives
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
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- A61K2800/592—Mixtures of compounds complementing their respective functions
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Abstract
The application discloses a composition for skin antibiosis and repair, a preparation method and application thereof, and belongs to the technical field of functional skin care products. The composition comprises: the composition comprises active peptide, nicotinamide adenine dinucleotide, calamine and water, wherein the pH value of the composition is 4.5-6. The composition can maintain weak acid environment of skin, inhibit bacteria, resist skin inflammation, prevent skin inflammation, promote skin cell proliferation and metabolism, supply energy for cell differentiation, and promote skin inflammation rapid repair.
Description
Technical Field
The application relates to a composition for skin antibiosis and repair, a preparation method and application thereof, belonging to the technical field of functional skin care products.
Background
Skin is the largest organ of the human body, and is not only an important barrier between the internal environment and the natural environment of the human body, but also an important organ for sensing natural signal stimulation. The skin has a sharp feel to temperature, humidity, light, air flow and pH, and plays an important role in preventing invasion of bacteria and viruses.
The pH of healthy skin is typically maintained between 4.0 and 6.5. The skin surface has a thin, slightly acidic protective layer, which protects the skin from harmful bacteria and viruses. The protective layer is formed by mixing free fatty acid discharged from sebaceous glands of skin with lactic acid and amino acid in sweat.
Physical factors, chemical factors, photodamage or allergy change the pH of the skin surface, and the change of the skin pH causes various skin problems, such as that the skin with acid is easy to generate acne and easy to secrete grease, and erythema and epidermitis are caused when the local slightly acidic environment of the skin reaches pH 2.0-3.0; the more alkaline skin with pH up to 8.0 is drier and prone to wrinkling.
Conventional skin care products generally use a weakly alkaline formulation for removing oil stains and metabolites from the skin surface, however, bacteria are easily attached and grown when the pH value of the skin is slightly alkaline, causing skin inflammation, leading to skin diseases.
Disclosure of Invention
In order to solve the problems, the composition for skin antibiosis and repair and the preparation method and application thereof are provided, the composition can maintain weak acid environment of skin, inhibit the generation of bacteria, have anti-inflammatory effect on skin, prevent the generation of skin inflammation, and simultaneously, the composition is beneficial to the proliferation and metabolism of skin cells, provides energy for cell differentiation and promotes the rapid repair of skin inflammation by adding active peptide, NAD and other components.
According to one aspect of the present application, there is provided a composition for skin antibacterial and repair comprising: the composition comprises active peptide, nicotinamide adenine dinucleotide, calamine and water, wherein the pH value of the composition is 4.5-6.
Optionally, the composition comprises, by mass, 0.1-0.25 parts of active peptide, 4-6 parts of nicotinamide adenine dinucleotide, 10-14 parts of calamine and 4000-6000 parts of water.
Optionally, the pH of the composition is 5.5 in parts by weight; the composition comprises 0.19 part of active peptide, 5 parts of nicotinamide adenine dinucleotide, 12 parts of calamine and 5000 parts of water.
Preferably, the calamine is a calamine suspension with a mass fraction of 5%.
Optionally, the active peptide comprises, in parts by mass: 0.05 to 0.1 part of carnosine, 0.02 to 0.1 part of palmitoyl tripeptide-1, 0.01 to 0.03 part of tripeptide-1 copper and 0.01 to 0.05 part of palmitoyl tetrapeptide-7;
preferably, the active peptide comprises the following components in parts by mass: 0.08 part of carnosine, 0.06 part of palmitoyl tripeptide-1, 0.02 part of tripeptide-1 copper and 0.03 part of palmitoyl tetrapeptide-7.
Optionally, the composition further comprises 30-40 parts by weight of cellulose;
preferably, the composition further comprises 35 parts by weight of cellulose.
Optionally, the composition further comprises 4-6 parts by weight of a calcium ion supplement; preferably 5 parts;
preferably, the calcium ion supplement is calcium citrate.
Optionally, the composition further comprises a pH adjuster.
Preferably, the pH regulator is a fruit acid.
Preferably, the pH regulator is malic acid, citric acid or tartaric acid.
Preferably, the pH adjuster is citric acid.
According to another aspect of the present application, there is provided a method for preparing a composition for skin antibacterial and repair as described in any one of the above, comprising the steps of: mixing the raw materials, adjusting pH to 4.5-6, and water-bathing at 25-45deg.C.
Optionally, the method comprises the following steps: mixing carnosine, palmitoyl tripeptide-1, tripeptide-1 copper, palmitoyl tetrapeptide-7, nicotinamide adenine dinucleotide, calamine and calcium citrate, adding cellulose to form a mixed solution, and regulating the mixed solution to 5.5 by adopting a pH regulator, and carrying out water bath at 36 ℃ for 30min to obtain the compound.
According to a further aspect of the present application there is provided the use of a composition according to any one of the above or a composition prepared by any one of the above methods for skin antibacterial and repair;
the application includes the application of the composition to the skin in at least one of a paste, an emulsion, and a spray.
The bioactive peptide biological macromolecule is positioned between a primary structure and a secondary structure at pH 5.5, and has the best bioactivity and penetrability; in addition, skin epidermal cells have a very sensitive pH dependence, and the pH value can effectively regulate the amount of peptide bound by each skin cell. When the pH of the skin microenvironment is about 5.5, 1300 lipids bind to 1 polypeptide, or each vesicle can encapsulate about 75 peptides, while one end of the active peptide is trans-membrane transferred into the cytoplasm and the other end remains in the extracellular space, thus localizing the active peptide in the membrane lipid bilayer, i.e. the active peptide has dual delivery capacity to transport chemicals, small molecules etc. of the substance to be transported into the cell. In addition, when the pH of the skin microenvironment is about 5.5, the negatively charged residues (Asp or Glu) are caused to protonate, the hydrophobicity of the active peptide is enhanced, the affinity of the active peptide to the lipid bilayer is further increased, the folding of the active peptide and the subsequent membrane insertion process are triggered, the absorption of the active peptide by the skin is facilitated, and the proliferation and metabolism of skin cells are promoted.
According to the invention, by adding carnosine, an immune barrier is provided for skin, and endocrine balance is maintained; tripeptide-1 copper can promote cell metabolism, stimulate cell proliferation, and has anti-wrinkle effect; palmitoyl tetrapeptide-7 has antioxidant and antiinflammatory effects.
The nicotinamide adenine dinucleotide (NAD for short) is added to be matched with carnosine, palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7, so that energy is provided for cell differentiation, proliferation of cells is promoted, skin metabolism is facilitated, and the anti-wrinkle agent has an anti-wrinkle function and is beneficial to rapid repair of skin inflammation; the reasonable proportion of NAD and calamine can regulate the pH of skin, maintain the acidic environment of skin, and simultaneously the calamine can maintain NAD as reduced NAD, thereby being beneficial to the absorption of NAD and improving the anti-inflammatory and antibacterial effects of the composition on skin.
According to the invention, through adding calamine, the calamine and palmitoyl tripeptide-1, tripeptide-1 copper and palmitoyl tetrapeptide-7 cooperate to enhance the absorption of tripeptide-1 copper and play an anti-inflammatory and antibacterial role on skin.
According to the invention, the cellulose is added, so that the long-chain structure of the cellulose can prevent the active peptide from aggregation, and the absorption of the active peptide is facilitated; calcium ions are added to supplement calcium ions lost by skin due to absorption of active peptide, so as to maintain cell membrane potential.
Benefits of the present application include, but are not limited to:
1. according to the composition for skin antibiosis and repair, the skin can maintain weak acid environment, inhibit bacteria, have anti-inflammatory effect on the skin, prevent skin inflammation, and meanwhile, by adding active peptide, NAD and other components, the composition is beneficial to proliferation and metabolism of skin cells, provides energy for cell differentiation and promotes rapid repair of skin inflammation.
2. The composition has no toxic or side effect, can be used for a long time, has better effect on skin stabilization, and can be used singly or matched with other skin care products as paste, emulsion or spray.
3. The preparation method of the composition has simple steps and is easy to operate.
Drawings
The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this application, illustrate embodiments of the application and together with the description serve to explain the application and do not constitute an undue limitation to the application. In the drawings:
FIG. 1 is a diagram of a skin cell repair process using composition 1# according to an embodiment of the present application.
Fig. 2 is a diagram of a skin cell repair process without any composition according to the examples of the present application.
Detailed Description
The present application is described in detail below with reference to examples, but the present application is not limited to these examples.
Unless otherwise indicated, both the starting materials and the catalysts in the examples of the present application were purchased commercially.
Example 1 preparation of the composition
The preparation method of the composition 1# comprises the following steps:
1) 0.08g of carnosine, 0.06g of palmitoyl tripeptide-1, 0.02g of tripeptide-1 copper, 0.03g of palmitoyl tetrapeptide-7, 5g of nicotinamide adenine dinucleotide, 240g of calamine suspension with the mass concentration of 5wt% and 5g of calcium citrate are mixed, added into 5000g of water, and 35g of cellulose is added to form a mixed solution;
2) Adding citric acid, and adjusting pH of the mixture to 5.5, and water-bathing at 36deg.C for 30min to obtain composition No. 1.
Preparing composition 2# -9#, composition D1# -D3# according to the preparation method of composition 1#, wherein composition 2# -9#, and composition D1# -D3# have the components shown in Table 1:
TABLE 1
Experimental example
The antibacterial effect test, the cortical cell differentiation inhibition effect test, the anti-inflammatory effect test and the cell viability test are respectively carried out on the composition 1# -9#, the composition D1# -D3#, and the test results are shown in the table 2, and the test method is as follows:
(1) Antibacterial effect test: taking propionibacterium acnes 24h culture, and diluting the culture with 0.03mol/L phosphate buffer solution to form 104-106 CFU/mL bacterial suspension; taking 1g of the composition, adding bacterial suspension, uniformly coating and mixing, recording absorbance at 450nm for 0h, culturing at 37 ℃ for 24h as a sample group, setting a blank group without the composition as a control group, recording absorbance at 450nm, and calculating the bacteriostasis rate, wherein the bacteriostasis rate= (OD value of the control group-OD value of the sample group)/OD value of the control group is multiplied by 100%.
(2) Anti-inflammatory effect: diluting Raw264.7 cells in optimal growth state to 7×10 4 -9×10 4 Inoculating 100 mu L of bacterial suspension in each hole into a 96-well plate, culturing in a 37 ℃ and 5% CO2 incubator, adding a composition with the mass concentration of 0.25% wt for 24 hours as a sample group, setting a blank group without the composition as a control group, taking supernatant, using an ELISA kit to detect the inhibition of an active substance on interleukin, and measuring absorbance at 450nm by using an enzyme-labeled instrument;
inhibition = (control OD value-sample OD value)/control OD value x 100%.
(3) Cell viability test: raw264.7 cells were cultured normally in RPMI1640 medium containing 10% fetal bovine serum at 5% and 37 ℃. When the cells grow to 90% density, they are grown according to 8×10 4 Inoculating the sample in 96-well plate at a density of/mL, adhering to the plate for 48h, adding a composition with a mass concentration of 0.25%wt for 24h to serve as a sample group, setting a blank group without the composition as a control group, adding CCK-8 reagent for incubation for 2h, and measuring absorbance at 450 nm;
cell viability% = sample group OD value/control group OD value x 100%.
TABLE 2
As shown in the table above, the composition 1# -9 of the present invention can maintain weak acid environment of skin, inhibit bacteria, resist inflammation of skin, prevent skin inflammation, maintain cell activity, promote proliferation and metabolism of skin cells, provide energy for cell differentiation, and promote rapid repair of skin inflammation.
In addition, the skin fibroblast scratch repair test was performed on composition 1#, and the experimental results are shown in fig. 1 and 2, in which fig. 1 is a skin cell repair process picture using composition 1#, and fig. 2 is a skin cell repair process picture without using any composition, and it can be seen that the cell proliferation and recovery effect using composition 1#, and thus the skin scratch repair speed was faster.
The foregoing is merely exemplary of the present application, and the scope of the present application is not limited to the specific embodiments, but is defined by the claims of the present application. Various modifications and changes may be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the technical ideas and principles of the present application should be included in the protection scope of the present application.
Claims (9)
1. A composition for skin antibacterial and repair, characterized in that it comprises: the composition comprises active peptide, nicotinamide adenine dinucleotide, calamine and water, wherein the pH value of the composition is 4.5-5.5;
the composition comprises, by mass, 0.1-0.25 part of active peptide, 4-6 parts of nicotinamide adenine dinucleotide, 10-14 parts of calamine and 4000-6000 parts of water;
the active peptide comprises the following components in parts by mass: 0.05 to 0.1 part of carnosine, 0.02 to 0.1 part of palmitoyl tripeptide-1, 0.01 to 0.03 part of tripeptide-1 copper and 0.01 to 0.05 part of palmitoyl tetrapeptide-7;
the composition also comprises 30-40 parts of cellulose by mass;
the composition also comprises 4-6 parts by mass of calcium ion supplement; the calcium ion supplement is calcium citrate.
2. The composition of claim 1, wherein the pH of the composition is 5.5; the composition comprises, by mass, 0.19 parts of active peptide, 5 parts of nicotinamide adenine dinucleotide, 12 parts of calamine and 5000 parts of water.
3. The composition of claim 1, wherein the active peptide comprises, in parts by weight: 0.08 part of carnosine, 0.06 part of palmitoyl tripeptide-1, 0.02 part of tripeptide-1 copper and 0.03 part of palmitoyl tetrapeptide-7.
4. A composition according to any one of claims 1 to 3, characterized in that it comprises 35 parts by mass of cellulose.
5. A composition according to any one of claims 1 to 3, wherein the composition further comprises a pH adjuster.
6. The composition of claim 5, wherein the pH adjuster is citric acid.
7. A method for preparing a composition for skin antibacterial and repairing according to any one of claims 1 to 6, comprising the steps of: mixing the raw materials, adjusting pH to 4.5-5.5, and water-bathing at 25-45deg.C.
8. The method of manufacturing according to claim 7, comprising the steps of: mixing carnosine, palmitoyl tripeptide-1, tripeptide-1 copper, palmitoyl tetrapeptide-7, nicotinamide adenine dinucleotide, calamine and calcium citrate, adding into water, adding cellulose to form a mixed solution, adjusting the mixed solution to 5.5 by adopting a pH regulator, and carrying out water bath at 36 ℃ for 30min to obtain the compound.
9. Use of a composition according to any one of claims 1 to 6 or a composition obtainable by a process according to any one of claims 7 to 8 for the preparation of a medicament for skin antibacterial and repair;
the application includes the application of the composition to the skin in at least one of a paste, an emulsion, and a spray.
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| CN110302091A (en) * | 2019-08-01 | 2019-10-08 | 深圳市兰亭科技股份有限公司 | A kind of anti aging effect composition of niacinamide-containing adenine-dinucleotide and its preparation method and application |
| CN113181081A (en) * | 2021-04-01 | 2021-07-30 | 科丝美诗(中国)化妆品有限公司 | Cosmetic containing anti-inflammatory oil control functional composition and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110302091A (en) * | 2019-08-01 | 2019-10-08 | 深圳市兰亭科技股份有限公司 | A kind of anti aging effect composition of niacinamide-containing adenine-dinucleotide and its preparation method and application |
| CN113181081A (en) * | 2021-04-01 | 2021-07-30 | 科丝美诗(中国)化妆品有限公司 | Cosmetic containing anti-inflammatory oil control functional composition and preparation method thereof |
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