CN116270289B - Ionic gel composition containing hydrolyzed actin and preparation method thereof - Google Patents
Ionic gel composition containing hydrolyzed actin and preparation method thereof Download PDFInfo
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- CN116270289B CN116270289B CN202310080981.4A CN202310080981A CN116270289B CN 116270289 B CN116270289 B CN 116270289B CN 202310080981 A CN202310080981 A CN 202310080981A CN 116270289 B CN116270289 B CN 116270289B
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- 102000007469 Actins Human genes 0.000 title claims abstract description 91
- 108010085238 Actins Proteins 0.000 title claims abstract description 91
- 239000000203 mixture Substances 0.000 title claims abstract description 63
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 239000002608 ionic liquid Substances 0.000 claims abstract description 60
- 239000002537 cosmetic Substances 0.000 claims abstract description 8
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 96
- 239000004475 Arginine Substances 0.000 claims description 35
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 35
- 229920001661 Chitosan Polymers 0.000 claims description 33
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 claims description 33
- 235000006408 oxalic acid Nutrition 0.000 claims description 32
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims description 31
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 31
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims description 31
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims description 31
- 239000000243 solution Substances 0.000 claims description 15
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims description 14
- 238000010438 heat treatment Methods 0.000 claims description 7
- 239000007864 aqueous solution Substances 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 37
- 230000003020 moisturizing effect Effects 0.000 abstract description 24
- 230000002195 synergetic effect Effects 0.000 abstract description 7
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 239000000499 gel Substances 0.000 description 79
- 239000000047 product Substances 0.000 description 38
- 210000001161 mammalian embryo Anatomy 0.000 description 20
- 238000012360 testing method Methods 0.000 description 20
- 241000252212 Danio rerio Species 0.000 description 19
- 210000002257 embryonic structure Anatomy 0.000 description 14
- 238000010586 diagram Methods 0.000 description 13
- 238000002474 experimental method Methods 0.000 description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 8
- 229960001231 choline Drugs 0.000 description 8
- 230000014759 maintenance of location Effects 0.000 description 8
- 239000003153 chemical reaction reagent Substances 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 238000001556 precipitation Methods 0.000 description 7
- 150000002500 ions Chemical class 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 5
- 239000013078 crystal Substances 0.000 description 5
- 239000013641 positive control Substances 0.000 description 5
- 239000002994 raw material Substances 0.000 description 5
- 230000000638 stimulation Effects 0.000 description 5
- 239000000872 buffer Substances 0.000 description 4
- 238000009472 formulation Methods 0.000 description 4
- BIVBRWYINDPWKA-VLQRKCJKSA-L Glycyrrhizinate dipotassium Chemical compound [K+].[K+].O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@H]1CC[C@]2(C)[C@H]3C(=O)C=C4[C@@H]5C[C@](C)(CC[C@@]5(CC[C@@]4(C)[C@]3(C)CC[C@H]2C1(C)C)C)C(O)=O)C([O-])=O)[C@@H]1O[C@H](C([O-])=O)[C@@H](O)[C@H](O)[C@H]1O BIVBRWYINDPWKA-VLQRKCJKSA-L 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000011068 loading method Methods 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000001133 acceleration Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 229940101029 dipotassium glycyrrhizinate Drugs 0.000 description 2
- 230000036571 hydration Effects 0.000 description 2
- 238000006703 hydration reaction Methods 0.000 description 2
- 230000003301 hydrolyzing effect Effects 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 229940068196 placebo Drugs 0.000 description 2
- 239000000902 placebo Substances 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000032798 delamination Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 230000001815 facial effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000000017 hydrogel Substances 0.000 description 1
- 239000000413 hydrolysate Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 230000031877 prophase Effects 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000002390 rotary evaporation Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/042—Gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/362—Polycarboxylic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/731—Cellulose; Quaternized cellulose derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/736—Chitin; Chitosan; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Emergency Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses an ionic gel composition containing hydrolyzed actin and a preparation method thereof, belonging to the field of cosmetics. The product takes the ionic gel prepared by the specific ionic liquid as a carrier to load the hydrolyzed actin, so that the stability and the long-acting property of the hydrolyzed actin can be effectively improved, and meanwhile, the ionic gel can be combined with the hydrolyzed actin to play a role in moisturizing and moisturizing the skin and enhancing the hydrolyzed actin, so that the product has a synergistic effect on the hydrolyzed actin; the product has the advantages of relieving effect, good biocompatibility, high safety to human bodies and strong use experience.
Description
Technical Field
The invention relates to the technical field of cosmetics, in particular to an ionic gel composition containing hydrolyzed actin and a preparation method thereof.
Background
The hydrolyzed actin is a cosmetic raw material obtained by re-hydrolyzing and refining actin in human or animal bodies, and is widely used as a moisturizing conditioner in moisturizing and moisturizing products because of the skin water locking capability.
However, since the activity of the hydrolyzed actin is high and is easily disturbed or affected by external factors, it is generally necessary to encapsulate or modify the hydrolyzed actin in order to ensure the long-acting property of the hydrolyzed actin. However, the existing carrier for hydrolyzing actin cannot take the release property and stability into account, has a certain inhibition effect on the efficacy of hydrolyzing actin, and meanwhile, some carriers and modification means do not fully consider biocompatibility and biodegradability, so that the prepared cosmetics have the problems of liquid phase layering and difficult cleaning, and have poor use experience.
Disclosure of Invention
Based on the defects existing in the prior art, the invention aims to provide the ionic gel composition containing the hydrolyzed actin, and the product takes the ionic gel prepared by specific ionic liquid as a carrier to load the hydrolyzed actin, so that the stability and the long-acting property of the hydrolyzed actin can be effectively improved, and meanwhile, the ionic gel can be combined with the hydrolyzed actin to play a role in moisturizing and moisturizing the skin and enhance the effect of synergism on the hydrolyzed actin; the product has the advantages of relieving effect, good biocompatibility, high safety to human bodies and strong use experience.
In order to achieve the above purpose, the invention adopts the following technical scheme:
an ionic gel composition containing hydrolyzed actin, which comprises the following components in parts by weight:
1-5 parts of hydrolyzed actin and 95-99 parts of ionic gel; the ionic gel comprises carboxymethyl chitosan, sodium carboxymethyl cellulose and ionic liquid; the ionic liquid comprises oxalic acid and arginine, and the molar ratio of the oxalic acid to the arginine is (1:3) - (2:1).
The ionic gel is a novel composite material formed by the mutual permeation of a solid phase with a three-dimensional network structure and a liquid phase of the ionic liquid, not only maintains the main characteristics of the ionic liquid, but also shows the excellent mechanical properties of the solid, and has long-acting stability and high-temperature stability. Meanwhile, the ionic gel contains abundant hydrophilic groups and hydrophilic structures, so that the ionic gel can absorb and store part of moisture and has certain moisturizing performance when being applied to cosmetics. In addition, compared with hydrogel, the ionic gel is rich in anions and cations, can generate hydration with water molecules, and further improves the moisturizing performance.
In the technical scheme, the inventor loads the hydrolyzed actin by taking the ionic gel as the carrier, so that the mutual synergistic effect of the carrier and the carrier is fully realized, the stability of the hydrolyzed actin is effectively increased, the original moisturizing and water supplementing capability of the hydrolyzed actin is also improved, the unique texture of the ionic gel can enable the hydrolyzed actin to be endowed with a comfortable and refreshing feel when the hydrolyzed actin contacts with skin, and the hydrolyzed actin has good biocompatibility and biodegradability, so that unpleasant experience or burden can not be brought to the skin.
However, when two natural polymer materials, such as carboxymethyl chitosan and sodium carboxymethyl cellulose, are directly adopted to prepare the ionic gel in combination with the ionic liquid, the stability of the product prepared by using different ionic liquids is different, and if the prepared ionic liquid is poor in stability, the effective load on the hydrolyzed actin is difficult to realize, even the quality of the ionic gel is influenced, and the original moisturizing performance of the hydrolyzed actin is inhibited. Meanwhile, as the polarities of the components and the hydrolyzed actin are not similar, the hydrolyzed actin is too little loaded, and the normal effect is difficult to be exerted; however, if the loading is excessive, the dispersibility of the whole ionic gel composition product is deteriorated, the appearance is layered or the raw material is not dissolved. Therefore, after research and development, the product prepared by adopting the formula provided by the invention has good stability and uniformity, and has good effects of moisturizing and relieving the active tissues of the human skin.
Preferably, the ionic liquid comprises oxalic acid and arginine, and the molar ratio of the oxalic acid to the arginine is (1:2) - (1:3).
The inventor experiments find that under the condition that the product formulation can form an effective form, arginine in the ionic liquid can have a synergistic effect with the hydrolyzed actin, so that the overall moisturizing effect is better.
Preferably, the ionic liquid is an aqueous solution of oxalic acid and arginine, and the mass concentration is 40-60 wt%.
More preferably, the mass concentration of the ionic liquid is 50wt%
The ionic liquid is taken as a part of the ionic gel, is not only a dissolvent of carboxymethyl chitosan and sodium carboxymethyl cellulose, but also is actually a decisive factor of the whole product form, and in order to ensure that the components of the ionic gel have proper fluidity after being mixed, the ionic liquid has certain viscosity and moisture retention when in use, and the ionic liquid is prepared into 40-60 wt% of aqueous solution with the best effect.
Preferably, the sum of the mass of the carboxymethyl chitosan and the mass of the sodium carboxymethyl cellulose accounts for 2-10% of the mass of the ionic gel composition containing the hydrolyzed actin, and the mass ratio of the carboxymethyl chitosan to the sodium carboxymethyl cellulose is (0.8-1.2): (0.8-1.2).
More preferably, the sum of the mass of the carboxymethyl chitosan and the mass of the sodium carboxymethyl cellulose accounts for 5% of the mass of the ionic gel composition containing the hydrolyzed actin, and the mass ratio of the carboxymethyl chitosan to the mass of the sodium carboxymethyl cellulose is 1:1.
The carboxymethyl chitosan and the sodium carboxymethyl cellulose have certain tackifying effect after being combined with the ionic liquid, so that on one hand, certain stable form and moisture retaining effect can be provided for the product, and on the other hand, better texture can be given to the product, and small fluidity cannot be maintained due to too little addition of the carboxymethyl chitosan and the sodium carboxymethyl cellulose, so that the product is not beneficial to exerting moisture retaining performance; however, the addition of the two components in an excessive amount results in deterioration of the stability of the whole product, and the phenomena such as precipitation and delamination are likely to occur, and the effect of suppressing the supported actin hydrolyzate is excellent.
Another object of the present invention is to provide a method for preparing the ionic gel composition containing hydrolyzed actin, comprising the steps of:
uniformly mixing the hydrolyzed actin, carboxymethyl chitosan, sodium carboxymethyl cellulose and ionic liquid, heating and stirring the mixture until the obtained solution is clear and uniform after the mixture is completely swelled at room temperature, and cooling the mixture to room temperature to obtain the ionic gel composition containing the hydrolyzed actin.
Preferably, the temperature at the time of heating is 60 to 65 ℃.
The preparation method of the ion gel composition containing the hydrolyzed actin has simple operation steps and can realize industrialized mass production.
It is a further object of the present invention to provide the use of said ionic gel composition comprising hydrolysed actin for the preparation of cosmetic skin care products.
Compared with the existing loaded or modified hydrolyzed actin, the ionic gel composition containing hydrolyzed actin has better stability and long-acting property, and the loaded ionic gel can realize the synergistic moisturizing effect with the hydrolyzed actin; the product has excellent moisturizing and soothing effects on skin, good biocompatibility and degradability, no toxicity or harm to human skin, high affinity, and excellent use value no matter being directly used or further processed into cosmetics and skin care products such as facial masks, face creams and essences.
The invention has the beneficial effects that the ionic gel composition containing the hydrolyzed actin is provided, and the product takes the ionic gel prepared by specific ionic liquid as a carrier to load the hydrolyzed actin, so that the stability and the long-acting performance of the hydrolyzed actin can be effectively improved, and meanwhile, the ionic gel can be combined with the hydrolyzed actin to play a role in moisturizing and moisturizing the skin, and has a synergistic effect on the hydrolyzed actin; the product has the advantages of relieving effect, good biocompatibility, high safety to human bodies and strong use experience.
Drawings
FIG. 1 is a schematic diagram of a control group 14 according to effect example 2 of the present invention.
FIG. 2 is a schematic diagram of a control group 15 according to effect example 2 of the present invention.
FIG. 3 is a schematic diagram of the control group 16 according to effect example 2 of the present invention.
FIG. 4 is a schematic diagram of the sample of the control group 17 of effect example 2 of the present invention.
FIG. 5 is a schematic diagram of the sample of the control group 18 of effect example 3 of the present invention.
FIG. 6 is a schematic diagram of a control group 19 according to effect example 3 of the present invention.
FIG. 7 is a schematic diagram of the control group 20 according to effect example 4 of the present invention.
FIG. 8 is a schematic diagram of a control group 21 according to effect example 4 of the present invention.
FIG. 9 is a schematic diagram of the sample of the control group 22 of effect example 4 of the present invention.
FIG. 10 is a schematic diagram of a control group 23 of effect example 4 of the present invention.
FIG. 11 is a schematic diagram showing a sample of the control group 24 of effect example 4 of the present invention.
FIG. 12 is a schematic diagram of a control group 25 according to effect example 5 of the present invention.
FIG. 13 is a schematic diagram showing a sample of a control group 27 of effect example 5 of the present invention.
FIG. 14 is a statistical chart showing the results of the test of the zebra fish embryo relaxation experiment of effect example 6 of the present invention.
FIG. 15 is a schematic representation of the test embryos of the invention after 1h of sample loading of example 3 at different concentrations in the test of the zebra fish embryo relaxation experiment of effect example 6.
FIG. 16 is a schematic of test embryos from a blank set of test experiments for soothing zebra fish embryos according to effect example 6 of the present invention.
Detailed Description
The present invention will be further described with reference to specific examples and comparative examples for better illustrating the objects, technical solutions and advantages of the present invention, and the object of the present invention is to be understood in detail, not to limit the present invention. All other embodiments, which can be made by those skilled in the art without the inventive effort, are intended to be within the scope of the present invention. The experimental reagents, raw materials and instruments designed in the practice and comparative examples of the present invention are common reagents, raw materials and instruments unless otherwise specified. The hydrolyzed actin used in the examples of the present invention was derived from a source foliar organism (CAS: 73049-73-7, LOT: Y14A11T 121060).
Example 1
According to one embodiment of the actin-containing ionic gel composition and the preparation method thereof, the ionic gel composition comprises the following components in parts by weight:
1 part of hydrolyzed actin and 99 parts of ionic gel; the ionic gel comprises 2.5 parts of carboxymethyl chitosan, 2.5 parts of sodium carboxymethyl cellulose and 94 parts of ionic liquid; the ionic liquid comprises oxalic acid and arginine, and the molar ratio of the oxalic acid to the arginine is 2:1, the ionic liquid is an aqueous solution of oxalic acid and arginine, and the mass concentration is 50wt%.
The preparation method of the ionic gel composition comprises the following steps:
uniformly mixing the hydrolyzed actin, carboxymethyl chitosan, sodium carboxymethyl cellulose and ionic liquid, heating to 60 ℃ after swelling completely at room temperature, magnetically stirring for 5 hours until the obtained solution is clear and uniform, and cooling to room temperature to obtain the ionic gel composition containing hydrolyzed actin.
Example 2
An embodiment of the present invention, an ion gel composition containing hydrolyzed actin and a preparation method thereof, differs from embodiment 1 only in that the ion gel composition comprises the following components in parts by weight:
2.5 parts of hydrolyzed actin and 97.5 parts of ionic gel; the ionic gel comprises 2.5 parts of carboxymethyl chitosan, 2.5 parts of sodium carboxymethyl cellulose and 92.5 parts of ionic liquid; the ionic liquid comprises oxalic acid and arginine, and the molar ratio of the oxalic acid to the arginine is 2:1, the ionic liquid is an aqueous solution of oxalic acid and arginine, and the mass concentration is 50wt%.
Example 3
An embodiment of the present invention, an ion gel composition containing hydrolyzed actin and a preparation method thereof, differs from embodiment 1 only in that the ion gel composition comprises the following components in parts by weight:
5 parts of hydrolyzed actin and 95 parts of ionic gel; the ionic gel comprises 2.5 parts of carboxymethyl chitosan, 2.5 parts of sodium carboxymethyl cellulose and 90 parts of ionic liquid; the ionic liquid comprises oxalic acid and arginine, and the molar ratio of the oxalic acid to the arginine is 2:1, the ionic liquid is an aqueous solution of oxalic acid and arginine, and the mass concentration is 50wt%.
Example 4
An embodiment of the present invention of an ionic gel composition containing hydrolyzed actin and a preparation method thereof differs from embodiment 2 only in that the molar ratio of oxalic acid to arginine in the ionic liquid is 1:2.
example 5
An embodiment of the present invention of an ionic gel composition containing hydrolyzed actin and a preparation method thereof differs from embodiment 2 only in that the molar ratio of oxalic acid to arginine in the ionic liquid is 1:3.
experimental example 1
In order to verify the initial stability of the ionic liquid, control groups 1 to 13 are set, and the specific examples are shown in table 1:
TABLE 1
| Control group | Component composition (molar ratio) |
| 1 | Oxalic acid: arginine=1:1 |
| 2 | Oxalic acid: arginine=1:2 |
| 3 | Oxalic acid: arginine=2:1 |
| 4 | Oxalic acid: arginine=3:1 |
| 5 | Oxalic acid: arginine=1:3 |
| 6 | Choline: citric acid=1:1 |
| 7 | Choline: oxalic acid =1:1 |
| 8 | Choline: oxalic acid=1:2 |
| 9 | Choline: oxalic acid=2:1 |
| 10 | Choline: arginine=1:1 |
| 11 | Choline: arginine=1:2 |
| 12 | Choline: arginine=2:1 |
| 13 | Choline: alanine=1:1 |
The preparation method of the ionic liquid of each control group comprises the following steps: after the raw material components were mixed according to the molar ratio listed in table 1, about 10g of the mixture was placed into 200mL of deionized water and mixed and stirred at 50 ℃ for 20 hours, the obtained solution was subjected to rotary evaporation at 55 ℃ under reduced pressure, and then dried at 60 ℃ under vacuum for 48 hours, so as to obtain the ionic liquid, and the preparation results of the control groups were recorded, as shown in table 2.
TABLE 2
| Control group | Preparation results |
| 1 | Clear liquid |
| 2 | Clear liquid |
| 3 | Clear liquid |
| 4 | Clear liquid |
| 5 | Clear liquid |
| 6 | Clear liquid |
| 7 | Clear liquid |
| 8 | Cannot form ionic liquid at room temperature |
| 9 | Cannot form ionic liquid at room temperature |
| 10 | With crystal precipitation |
| 11 | With crystal precipitation |
| 12 | With crystal precipitation |
| 13 | With crystal precipitation |
It can be seen that the formulations of control groups 1-7 were successful in formulating ionic liquids with room temperature stability.
Then, respectively carrying out a long-acting stability experiment and an acceleration stability experiment on the products of the control group 1-9 in parallel, wherein the experiment duration is 30 days, and the conditions of the long-acting stability experiment are 25+/-2 ℃ and RH 60+/-5%; the conditions for the accelerated stability test were 40.+ -. 2 ℃ and RH 75.+ -. 5%, and the test results are shown in Table 3.
TABLE 3 Table 3
As can be seen from table 3, the control groups 1 and 8 showed crystal precipitation after long-acting and acceleration experiments, and were poor in stability.
Experimental example 2
In order to verify the preference of the dosage of carboxymethyl chitosan and sodium carboxymethyl cellulose in the ionic gel, oxalic acid and arginine are used according to the molar ratio of 2:1 preparing an ionic liquid with the mass concentration of 50wt%, adding specific amounts of carboxymethyl chitosan and sodium carboxymethyl cellulose (the mass ratio of the two is 1:1) into the ionic liquid, heating to 60 ℃ after the ionic liquid is completely swelled at room temperature, magnetically stirring the mixture for 5 hours until the obtained solution is clear and uniform, and cooling the solution to room temperature to obtain control groups 14-17, wherein the total mass content of the carboxymethyl chitosan and the sodium carboxymethyl cellulose in the four groups is 2%, 5%, 10% and 20%, respectively, and the test results are shown in figures 1-4. As can be seen, the solution in the control group 14 is transparent and clear, and the addition amount of carboxymethyl chitosan and sodium carboxymethyl cellulose is small, so that the overall fluidity is good, the viscosity is relatively low, and the moisturizing effect is low; the solution of the control group 15 is still clear, but the viscosity is improved compared with that of the control group 14, and the moisturizing capability of the product is also improved; the control group 16 had a higher viscosity and showed few insoluble substances and some air bubbles, while the control group 17 had more insoluble substances, which made it difficult to carry out further loading of the hydrolyzed actin. Experimental contrast shows that the introduction of carboxymethyl chitosan and sodium carboxymethyl cellulose cannot be too little or too much, and when the mass content of the additive is 2-10%, especially 5%, the ionic gel composition can be endowed with proper viscosity and moisture retention performance, the phenomena of precipitation and the like can be avoided, and the actin is hydrolyzed by the effective load.
Experimental example 3
In order to verify the preference of the concentration of the ionic liquid in the ionic gel, oxalic acid and arginine are used according to the molar ratio of 2:1 preparing an ionic liquid with the mass concentration of 100wt%, then adding carboxymethyl chitosan and sodium carboxymethyl cellulose with the mass content of 5% (the mass ratio of the carboxymethyl chitosan to the sodium carboxymethyl cellulose is 1:1) to completely swell at room temperature, heating to 60 ℃ and magnetically stirring for 5 hours until the obtained solution is clear and uniform, and cooling to room temperature to obtain a control group 18; according to the above method, 100wt% of the ionic liquid is replaced by pure water to prepare a control group 19, and as shown in fig. 5 and 6, it can be seen that the control group 18 has more insoluble substances, which indicates that the solubility of carboxymethyl chitosan and sodium carboxymethyl cellulose in pure oxalic acid and arginine is not ideal, and pure ionic liquid cannot further enable the ionic gel to stably contain enough carboxymethyl chitosan and sodium carboxymethyl cellulose; the carboxymethyl chitosan and sodium carboxymethyl cellulose in the control group 19 products are completely dissolved, but relatively low in viscosity and too high in fluidity, and still cannot maintain excellent morphology and moisturizing performance.
Experimental example 4
In order to explore the proper load of the hydrolyzed actin in the ionic gel composition, control groups 20 to 24 with the mass content of the hydrolyzed actin of 1 percent, 2.5 percent, 5 percent, 7.5 percent and 10 percent are respectively prepared according to the formula (except the addition amount of the hydrolyzed actin) and the method of the example 1, and the test results are shown in figures 7 to 11, wherein the control group 20 product is light yellow, clear and transparent, has moderate viscosity, low fluidity and ideal moisturizing capability; the viscosity of the control group 21-22 products is slightly higher than that of the control group 20, but the shape is not changed excessively, and the products still keep clear and transparent; the control group 23 product is in a yellow opaque gel form, and it can be seen that the partially hydrolyzed actin is not completely dispersed, while the control group 24 product is darker in color and higher in turbidity, and most of the hydrolyzed actin is not dispersed, so that the addition amount of the hydrolyzed actin is optimal by 1-5% in the preparation of the ionic gel composition of the invention.
Subsequently, the samples were subjected to a moisture retention test: the control group 20-22 products were placed in a constant weight glass dish with an equivalent of 1g, placed for 12 hours at 37+ -1deg.C under RH 75+ -5%, and the remaining mass of the products was measured at 1h, 2h, 4h, 7h and 12h, and the mass retention (Nh test mass/initial mass 1 g). Times.100% was calculated, and the test was repeated in parallel for 3 times to average, in which a blank group was also set, i.e., an ionic gel composition product containing no actin hydrolysate was prepared as in example 1, and the same test was performed with the same mass of pure water to confirm the loss of water in the environment. The test results are shown in Table 4.
TABLE 4 Table 4
As can be seen from the table, compared with pure water, the mass retention rate of the ionic gel without the hydrolyzed actin in the blank group is obviously higher, and the mass retention rate of the control groups 20-22 is further increased, which proves that the ionic gel composition containing the hydrolyzed actin, which is formed by ionic liquid, carboxymethyl chitosan and the like, has the water locking effect due to the hydration of the ionic gel formed by the ionic liquid, carboxymethyl chitosan and the like due to the abundant ionic effect on one hand, and the hydrolyzed actin seems to have the synergistic gain effect on the water locking effect of the ionic gel on the other hand, so that the moisture retention of the whole ionic gel composition is better.
Experimental example 5
As can be seen from the test results of experimental example 1, there was a difference in stability of different ionic liquids, the ionic gel composition was prepared with 2.5% of the amount of hydrolyzed actin, 5% of the amount of carboxymethyl chitosan and sodium carboxymethyl cellulose added and 50% of the mass concentration of the ionic liquid according to the test results of experimental examples 2 to 4, and the ionic liquid formulations used in the ionic gel composition were prepared according to the following table 5, respectively, to control 25 to 30 ionic gel composition products according to the test results of table 3 (when the molar ratio of oxalic acid to arginine was 2:1, experimental example 4 was already performed, and will not be repeated here). As a result, the results of the comparison group 25 and 27 products (i.e., the molar ratio of oxalic acid to arginine in the ionic liquid is 1:2 and 1:3) are shown in fig. 12 and 13, and the whole product is clear and transparent, has a certain viscosity and weak fluidity, which indicates that the ionic liquid used in the formulation can still maintain good moisture retention and morphological effects after being further configured into ionic gel and loaded with hydrolyzed actin, while other ionic liquids which are separately stabilized are configured into ionic gel compositions, part of the products are opaque, and all cannot completely form gel, which indicates that the ionic liquid cannot effectively prepare ionic gel and load hydrolyzed actin.
Further, the products of the control groups 25 and 27 were subjected to a moisturizing test with reference to experimental example 4, and a blank group and a pure water group were also set, and the results are shown in table 6.
TABLE 5
TABLE 6
It can be seen that the results of each group of tests and the results of table 4 are not greatly different, but the moisturizing effect of the control group 27 product is more remarkable than that of the control group 25 and the control group 21 in experimental example 4, which indicates that the higher the arginine content in the ionic liquid, the more synergistic effect can be generated with the hydrolyzed actin, so that the moisturizing effect of the prepared ionic gel composition is better, but the addition amount of arginine cannot be excessive, otherwise, the ionic gel composition cannot be molded after the hydrolyzed actin is loaded.
Experimental example 6
To verify the soothing effect of the ionic gel composition of the present invention when in use, the products of examples 1 to 5 were used to perform zebra fish embryo soothing experiments:
(1) Reagents and equipment for experiments:
example preparation of a product, a blank gel control (ion gel composition without hydrolyzed actin was prepared as in example 1), commercially available zebra fish embryo buffer (mass ratio sodium chloride: sodium bicarbonate: potassium chloride: calcium chloride=7:0.4:0.1:0.235), commercially available SDS, commercially available merocyanin reagent, commercially available dipotassium glycyrrhizinate, cell incubator, 96 well plate, video recorder.
(2) Reagent pre-stage configuration:
the products prepared in each example and the blank gel group control are diluted into solutions with the mass concentration of 2.5%, 1.25% and 0.625% respectively by using zebra fish embryo buffer, and then SDS (sodium dodecyl sulfate) modeling agent with the concentration of 0.74% and dipotassium glycyrrhizinate positive control with the concentration of 0.09% are prepared.
(3) Prophase collection and culture of zebra fish embryo
In an adult wild zebra fish feeding and special-purpose cultivation system (pH value is 7.0-8.0, conductivity range is 500-800 mu S/cm, room temperature is 26+/-1 ℃, water temperature is 28+/-1 ℃, photoperiod is 14h, light and 10h darkness) 3-18 months of fed female zebra fish are fished into a mating jar in the afternoon of the day before collecting the eggs, and a partition plate is inserted. The partition board in the middle of the cylinder is pulled out from 9 to 9 in the morning and the embryo is collected after 15 min. Embryos are placed in a constant temperature incubator (28+ -0.5 ℃) and added with embryo buffer to be cultured to 24hpf (Prim-5 stage), and normal-developing embryos are selected for experiments.
(4) The experimental steps are as follows:
groups were set up, including 1 blank group (i.e., control group without any experimental reagent added), 1 model group, 1 positive control group, and the remaining 15 experimental groups, all groups were tested using 96-well plates, 3 duplicate wells were set up, 5 zebra fish embryos (zebra fish embryo buffers were contained in the wells) per well, and the 96-well plates were placed in a cell incubator for 1h.
After embryo culture is completed, SDS (sodium dodecyl sulfate) modeling agent is added into the model group, dipotassium glycyrrhizate positive control substance is added into the positive control group, no reagent is added into the blank group, three solutions with different concentrations are respectively prepared into the remaining 15 groups by adding 5 examples and blank gel group control substances, and after 15min, recording of each embryo video is started for 30s.
The number of spin movements of each group of embryos was counted, and the results were imported into an Excel table for statistical analysis, and their relative stimulus intensities were analyzed using the following formula. Results were expressed as mean ± standard error (mean ± SEM), and significance was analyzed using One-way ANOVA between the different groups using GraphPad Prism 8.0, and P <0.05 was determined to be significantly different.
Relative stimulation intensity = number of spin movements of individual embryos/(total number of spin movements of placebo/total number of placebo).
The test results are shown in table 7 and fig. 14.
TABLE 7
Zebra fish embryo has strong sensitivity, the embryo is transparent in whole body in early development stage, and spin movement can be generated when the embryo develops to 24 hpf. When the fish embryo is subjected to chemical stimulation, the number of spinning movements generated by the fish embryo can be increased; when the number of spin movements was significantly reduced after the sample was added, it was considered to have a soothing effect on zebra fish embryos.
From the test results, the relative stimulus intensity of the zebra fish embryo of the model group and the zebra fish embryo of the blank group is 8.56 (# # P < 0.001); the relative stimulation intensity of the zebra fish embryo of the positive control group and the zebra fish embryo of the blank group is 1.39; compared with the blank gel control group, the relative stimulation intensities of the blank gel control group are 6.28,3.44 and 2.18 respectively along with the increase of the concentration, and the relative stimulation intensities of the groups of the examples are obviously reduced (P <0.05, P <0.01 and P < 0.001) and are further reduced than the blank gel control group, so that the ionic gel composition has a relieving effect on the spin movement of zebra fish embryos, and the hydrolyzed actin in the product can further promote the relieving effect of the ionic gel.
In addition, morphological observation of zebra fish embryos after 1h of addition of the test solution revealed that the middle of these embryos remained in a yellow spot, indicating that they were still in a viable state (as exemplified in fig. 15 corresponding to example 3), consistent with the blank set of fig. 16, indicating that the ionic gel composition of the present invention was biosafety and did not relax spin movements of the embryos by killing the embryos.
Finally, it should be noted that the above embodiments are only for illustrating the technical solution of the present invention and not for limiting the scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solution of the present invention may be modified or substituted equally without departing from the spirit and scope of the technical solution of the present invention.
Claims (6)
1. An ionic gel composition containing hydrolyzed actin is characterized by comprising the following components in parts by weight:
1-5 parts of hydrolyzed actin and 95-99 parts of ionic gel; the ionic gel comprises carboxymethyl chitosan, sodium carboxymethyl cellulose and ionic liquid; the sum of the mass of the carboxymethyl chitosan and the mass of the sodium carboxymethyl cellulose accounts for 2-10% of the mass of the ion gel composition containing the hydrolyzed actin, and the mass ratio of the carboxymethyl chitosan to the sodium carboxymethyl cellulose is (0.8-1.2): (0.8-1.2); the ionic liquid comprises oxalic acid and arginine, and the molar ratio of the oxalic acid to the arginine is (1:2) - (1:3); the mass concentration of the ionic liquid is 40-60wt%.
2. The actin-containing ionic gel composition according to claim 1, wherein the ionic liquid is an aqueous solution of oxalic acid and arginine, and the mass concentration of the ionic liquid is 50wt%.
3. The actin-containing ionic gel composition of claim 1, wherein the sum of the mass of carboxymethyl chitosan and sodium carboxymethyl cellulose is 5% of the mass of the actin-containing ionic gel composition, and the mass ratio of carboxymethyl chitosan to sodium carboxymethyl cellulose is 1:1.
4. A method for preparing an actin-containing ionic gel composition according to any one of claims 1 to 3, comprising the steps of:
uniformly mixing the hydrolyzed actin, carboxymethyl chitosan, sodium carboxymethyl cellulose and ionic liquid, heating and stirring the mixture until the obtained solution is clear and uniform after the mixture is completely swelled at room temperature, and cooling the mixture to room temperature to obtain the ionic gel composition containing the hydrolyzed actin.
5. The method for preparing an ionic gel composition containing hydrolyzed actin according to claim 4, wherein the temperature is 60-65 ℃ when heating.
6. Use of an ionic gel composition containing hydrolyzed actin according to any one of claims 1 to 3 in the preparation of a cosmetic skin care product.
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| KR100285396B1 (en) * | 1999-03-30 | 2001-03-15 | 서경배 | A composite powder, a method for preparing of the same and make-up cosmetics containing the same |
| CN107951773A (en) * | 2017-12-14 | 2018-04-24 | 广州赛莱拉干细胞科技股份有限公司 | A kind of anti-oxidant skin care item and preparation method thereof |
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| KR100285396B1 (en) * | 1999-03-30 | 2001-03-15 | 서경배 | A composite powder, a method for preparing of the same and make-up cosmetics containing the same |
| CN107951773A (en) * | 2017-12-14 | 2018-04-24 | 广州赛莱拉干细胞科技股份有限公司 | A kind of anti-oxidant skin care item and preparation method thereof |
| JP2020075911A (en) * | 2018-10-23 | 2020-05-21 | 日光ケミカルズ株式会社 | Novel ascorbic acid derivative, cosmetic composition comprising the same and cosmetic composition comprising them |
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