CN113583261B - 一种胶原/聚乙烯醇/掺铁介孔生物玻璃和聚乙烯醇双层水凝胶材料及其制备方法 - Google Patents

一种胶原/聚乙烯醇/掺铁介孔生物玻璃和聚乙烯醇双层水凝胶材料及其制备方法 Download PDF

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CN113583261B
CN113583261B CN202110950313.3A CN202110950313A CN113583261B CN 113583261 B CN113583261 B CN 113583261B CN 202110950313 A CN202110950313 A CN 202110950313A CN 113583261 B CN113583261 B CN 113583261B
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collagen
polyvinyl alcohol
iron
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周恬
郑凯
张晓梦
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Ninth Peoples Hospital Shanghai Jiaotong University School of Medicine
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Abstract

本发明涉及胶原/聚乙烯醇/掺铁生物玻璃纳米颗粒水凝胶的制备方法,其制备过程包括：胶原溶液、聚乙烯醇溶液共混，加入经溶胶‑凝胶法制得的掺铁介孔生物玻璃，放置于‑20℃下24小时形成水凝胶后，再添加一层聚乙烯醇溶液，再次放置于‑20℃下24小时可形成双层水凝胶。本发明所制备的胶原/聚乙烯醇/掺铁介孔生物玻璃和聚乙烯醇双层水凝胶材料在医学领域有很大的应用前景，其双层结构能有效引导牙槽骨再生并阻挡牙龈长入，可作为引导牙周组织再生膜用于口腔牙周组织再生及治疗种植体周围炎等。

Description

一种胶原/聚乙烯醇/掺铁介孔生物玻璃和聚乙烯醇双层水凝 胶材料及其制备方法
技术领域
本发明涉及一种胶原/聚乙烯醇/掺铁介孔生物玻璃和聚乙烯醇双层水凝胶材料及其制备方法，所述双层水凝胶可用于口腔牙周组织再生。
背景技术
构建针对牙周组织缺损功能性重建的引导牙周组织再生(GTR)材料已成为临床再生医学和生物材料领域中重点研究的方向之一。理想的GTR材料需要同时兼有主动诱导牙周组织修复再生的能力、屏障牙龈上皮根向移行、可调控降解速度和生物安全性能。胶原制备的可降解GTR/GBR材料，已被证明能促进人牙周膜细胞和成骨细胞的生长，且对牙周缺损有一定的修复效果，但仍存在力学强度不足和不能特异性调控细胞(促进牙周膜/成骨细胞增殖分化，抑制牙龈细胞粘附生长)的能力。通过在胶原中加入适量聚乙烯醇(PVA),不仅能增强材料的力学性能，还能发挥特异性调控细胞的能力。前期研究我们发现了PVA和胶原在一定比例下最适合于细胞生长，而单纯PVA则能抑制细胞粘附，所以本发明采用了双层水凝胶结构。其中一层采用PVA和胶原复合，并引入生物活性材料掺铁生物玻璃，胶原中引入生物玻璃能够显著提升人牙周膜细胞的增殖能力和其成骨基因的表达；引入铁成分能进一步增强生物玻璃的细胞活性和纤连蛋白的黏附能力，并参与巨噬细胞的免疫调节功能，在局部炎症反应和修复再生的平衡中发挥关键作用。另一层采用单纯PVA水凝胶，模拟结合上皮的作用，减少牙龈细胞的附着和长入。该双层水凝胶材料能发挥双向调节作用，最终为牙周组织缺损功能性重建提供重要保证。
发明内容
为了解决上述一般GTR膜存在的问题，本发明提供了一种用于牙周组织再生修复的新型的双层水凝胶膜材料，并使之具备优异的理化性能和生物相容性。
因此，本发明的第一个方面提供一种用于引导牙周组织再生的胶原/壳聚糖/掺铁生物玻璃纳米颗粒水凝胶材料，其特征在于，所述材料具有双层结构，其中一层包含胶原和聚乙烯醇，另一层由聚乙烯醇组成。
优选的，所述包含胶原和聚乙烯醇的层中还包含掺铁介孔生物玻璃。
进一步优选的，胶原、聚乙烯醇和掺铁介孔生物玻璃纳米颗粒的重量比为(20-1)∶(20-1)∶(0-10％)；优选为1∶1∶2-10％。
进一步优选的，所述胶原为鱼胶原。
本发明的第二方面提供一种制备上述任一项所述用于引导牙周组织再生的胶原/壳聚糖/掺铁生物玻璃纳米颗粒水凝胶材料的方法，其特征在于，所述方法包括如下步骤：
步骤1，提供PVA溶液和胶原溶液，
步骤2，将PVA溶液和胶原溶液混合，然后加入或不加入掺铁介孔生物玻璃，放置后得到第一水凝胶；
步骤3，在第一水凝胶表面加入相同体积的PVA溶液，放置后得到双层水凝胶。；
优选的，所述胶原为鱼胶原，溶剂为乙酸溶液。
进一步优选的，所述PVA溶液中，溶剂为水。
进一步优选的，所述掺铁生物玻璃纳米颗粒采用溶胶-凝胶法制备。
进一步优选的，所述PVA溶液中，PVA浓度为5％-10％。
进一步优选的，所述胶原溶液中，胶原浓度为1％-10％。
进一步优选的，所述聚乙烯醇数据分子量为1万至40万，更优选为30万至40万
进一步优选的，所述掺铁介孔生物玻璃采用溶胶-凝胶法制备
进一步优选的，所述掺铁介孔生物玻璃纳米颗粒中，介孔生物玻璃纳米颗粒材料的浓度为0-20g/100ml
进一步优选的，凝胶中，胶原、聚乙烯醇和掺铁介孔生物玻璃纳米颗粒的重量比为(20-1)∶(20-1)∶(0-10％)；更优选为1∶1∶2-10％
本发明的第三个方面是提供一种PVA/Col/Fe-MBG和PVA双层水凝胶材料，其中，所述胶原/壳聚糖/掺铁介孔生物玻璃水凝胶材料包括PVA、胶原和掺铁介孔生物玻璃纳米颗粒。
所述胶原/聚乙烯醇/掺铁介孔生物玻璃水凝胶材料优选为由本发明第二个方面所述方法制备。
本发明的优点在于：
(1)胶原具有良好的生物相容性，能促进人牙周膜细胞和成骨细胞的生长，且对牙周缺损有一定的修复效果。
(2)聚乙烯醇能与天然生物材料复合制备的水凝胶，并有效改善材料的力学强度和降解性能。纯聚乙烯醇水凝胶能够特异性抑制牙龈细胞粘附生长的能力来防止牙龈长入。
(3)本发明介孔生物活性玻璃中，引入铁成分能进一步增强生物玻璃的细胞活性和纤连蛋白的黏附能力，并参与巨噬细胞的免疫调节功能，在局部炎症反应和修复再生的平衡中发挥关键作用
(4)本发明构建双层胶原基复合支架，一层为PVA/Col/Fe-MBG以增强材料的生物活性，通过直接诱导和免疫调控促进支架主动诱导组织再生能力，另一层为PVA水凝胶层，模拟结合上皮的作用，减少牙龈细胞的附着和长入，最终为牙周组织缺损功能性重建提供重要保证。
附图说明
图1是实施例一制得的PVA/Col和PVA双层凝胶的扫描电镜图；
图2是实施例二制得的PVA/Col/2％Fe-MBG和PVA双层凝胶的扫描电镜图；
图3是实施例三制得的PVA/Col/10％Fe-MBG和PVA双层凝胶的扫描电镜图；
图4是本申请实施例制得的双层凝胶的红外图谱；
图5是本申请实施例制得的双层凝胶的溶胀性实验结果图。
具体实施方式
下面结合具体实例，进一步阐述本发明，需理解这些实例仅用于说明本发明而不用于限制本发明的范围。此外应理解，在阅读了本发明讲授的内容之后，本领域技术人员可以对本发明作各种改动或修改，这些等价形式同样落于本申请所附权利要求书所限定的范围。
实例一：
将PVA(MW：145000g/mol)溶于去离子水中得到浓度为10％wt％的PVA溶液；将鱼胶原溶于0.5M乙酸溶中，得到浓度为1％wt％的鱼胶原溶液。将PVA和鱼胶原按照1:1体积混合，置入-20℃静置24h后溶液变成凝胶。再在表面加入同样体积的10％wt％的PVA溶液，置入-20℃静置24h后变成PVA/Col和PVA双层凝胶。
实例二：
将PVA(MW：145000g/mol)溶于去离子水中得到浓度为10％wt％的PVA溶液；将鱼胶原溶于0.5M乙酸溶中，得到浓度为1％wt％的鱼胶原溶液。将PVA和鱼胶原按照1:1体积混合，其中加入2％Fe-MBG，置入-20℃静置24h后溶液变成凝胶。其中2％Fe-MBG的制备过程为：将4g Pluronic P123溶解在60mL乙醇中，加入7.2mL原硅酸四乙酯到溶液中，搅拌30分钟，加入3.04g四水硝酸钙，搅拌30分钟，加入0.38g的九水合硝酸铁(III)搅拌24小时，蒸发诱导7天。将干燥的凝胶在700℃下煅烧3小时。研磨获得尺寸低于100μm的2％Fe-MBG。再在表面加入同样体积的10％wt％的PVA溶液，置入-20℃静置24h后变成PVA/Col/2％Fe-MBG和PVA双层凝胶。
实例三：
将PVA(MW：145000g/mol)溶于去离子水中得到浓度为10％wt％的PVA溶液；将鱼胶原溶于0.5M乙酸溶中，得到浓度为1％wt％的鱼胶原溶液。将PVA和鱼胶原按照1:1体积混合，其中加入10％Fe-MBG，置入-20℃静置24h后溶液变成凝胶。其中10％Fe-MBG的制备过程为：将4g Pluronic P123溶解在60mL乙醇中，加入7.2mL原硅酸四乙酯到溶液中，搅拌30分钟，加入2.18g四水硝酸钙搅拌，加入1.86g九水合硝酸铁(III)搅拌24小时，蒸发诱导7天。将干燥的凝胶在700℃下煅烧3小时。研磨获得尺寸低于100μm的10％Fe-MBG。再在表面加入同样体积的10％wt％的PVA溶液，置入-20℃静置24h后变成PVA/Col/10％Fe-MBG和PVA双层凝胶。
通过红外图谱(FTIR)(如图4)和溶胀性实验(如图5)我们可以发现，该水凝胶材料成功掺入了胶原，PVA和Fe-MBGN成分，且具有较好稳定性，有利于材料在一定时间内发挥效能。
以上对本发明的具体实施例进行了详细描述，但其只是作为范例，本发明并不限制于以上描述的具体实施例。对于本领域技术人员而言，任何对本发明进行的等同修改和替代也都在本发明的范畴之中。因此，在不脱离本发明的精神和范围下所作的均等变换和修改，都应涵盖在本发明的范围内。

Claims (7)

1.一种用于引导牙周组织再生的胶原/聚乙烯醇/掺铁生物玻璃纳米颗粒水凝胶材料，其特征在于，所述材料具有双层结构，其中一层包含胶原和聚乙烯醇，另一层由聚乙烯醇组成；
所述包含胶原和聚乙烯醇的层中还包含掺铁介孔生物玻璃；
所述胶原、聚乙烯醇和掺铁介孔生物玻璃纳米颗粒的重量比为1∶1∶2-10％；
所述掺铁介孔生物玻璃纳米颗粒尺寸低于100μm。
2.如权利要求1所述的一种用于引导牙周组织再生的胶原/聚乙烯醇/掺铁生物玻璃纳米颗粒水凝胶材料，其特征在于，所述胶原为鱼胶原。
3.一种制备权利要求1或2所述用于引导牙周组织再生的胶原/聚乙烯醇/掺铁生物玻璃纳米颗粒水凝胶材料的方法，其特征在于，所述方法包括如下步骤：
步骤1，提供PVA溶液和胶原溶液；
步骤2，将PVA溶液和胶原溶液混合，然后加入掺铁介孔生物玻璃，放置后得到第一水凝胶；
步骤3，在第一水凝胶表面加入PVA溶液，放置双层水凝胶。
4.根据权利要求3所述的方法，其特征在于，所述胶原为鱼胶原，溶剂为乙酸溶液。
5.根据权利要求3所述的方法，其特征在于，所述PVA溶液中，溶剂为水。
6.根据权利要求3所述的方法，其特征在于，所述掺铁生物玻璃纳米颗粒采用溶胶-凝胶法制备。
7.如权利要求1所述用于引导牙周组织再生的胶原/聚乙烯醇/掺铁生物玻璃纳米颗粒水凝胶材料的制备方法，其特征在于，包括如下步骤，将PVA溶于去离子水中得到PVA溶液；将鱼胶原溶乙酸溶中，得到浓度鱼胶原溶液，将PVA和鱼胶原按照1:1体积混合，其中加入Fe-MBG，低温静置后溶液变成凝胶，在表面加入同样体积的PVA溶液，低温静置后变成PVA/Col/Fe-MBG和PVA双层凝胶。
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