CN113577162A

CN113577162A - Oral spray containing 10-HDA and preparation method thereof

Info

Publication number: CN113577162A
Application number: CN202110813969.0A
Authority: CN
Inventors: 苏静; 王丽; 王瑞明; 徐子淇; 王蕾蕾; 杨金硕; 吴文杰
Original assignee: Qilu University of Technology
Current assignee: Qilu University of Technology
Priority date: 2021-07-27
Filing date: 2021-07-27
Publication date: 2021-11-02

Abstract

The invention provides an oral spray containing 10-HDA and a preparation method thereof. The oral spray containing 10-HDA is prepared from the following raw materials in parts by weight: 8-15 parts of mint, 8-15 parts of honeysuckle, 8-15 parts of dandelion, 8-15 parts of houttuynia cordata, 0.5-1.5 parts of sodium benzoate, 1-2 parts of 10-hydroxy-2-decenoic acid (10-HDA), 0.5-2 parts of menthol, 1-3 parts of vitamin C, 2-10 parts of cane sugar and 2500 parts of water 1500-. The oral spray prepared by 10-HDA and other important components can effectively inhibit bacterial microorganisms from breeding in the oral environment, protect the oral cavity to a great extent, improve the oral disease state and promote the normal repair of oral tissues, thereby maintaining the health of human bodies.

Description

Oral spray containing 10-HDA and preparation method thereof

Technical Field

The invention relates to an oral spray containing 10-HDA and a preparation method thereof, belonging to the technical field of spray processing.

Background

Oral health is an important sign of human health and has important effects on the whole body health. However, various lesions, such as ulcers, wounds, etc., often occur to the tissues within the oral cavity. Due to the complex environment in the oral cavity, there are many bacteria, which results in a slow healing rate of the wound. In this case, the body's resistance and immunity are easily affected. Therefore, various medicines or health care products for treating oral diseases are rushed out in the market. However, because of the long-term, widespread use of these therapeutic agents by patients, the bacteria in the oral cavity have already developed resistance and the results are less than ideal when these agents are reused. Therefore, there is an urgent need for a new product that can be used for the prevention and treatment of oral diseases.

Chinese patent document CN111388560A discloses a traditional Chinese medicine composition for inhibiting formation of dental plaque, a preparation method and application thereof. According to the in vitro antibacterial activity of the extracting solution, five kinds of medicine and food resources such as houttuynia, dandelion, mint, honeysuckle, liquorice and the like are preferably selected, and then the five kinds of medicine and food resources are optimally combined into a compound with better taste according to the compatibility rule of the traditional Chinese medicines. The action principle of the invention is that the phytochemical extract is utilized to adjust the dynamic balance of flora in the oral cavity, and the growth and adhesion of pathogenic bacteria such as streptococcus mutans and the like are inhibited to avoid forming dental plaque, thereby relieving periodontitis and halitosis. The composition can be developed into products such as mouth wash, oral spray and solid beverage. But the traditional Chinese medicine composition has limited bacteriostatic effect; the oral therapeutic drugs are widely used by patients, and the drug resistance to the common drugs is generated, so that the expected effect is far from being achieved.

How to further effectively improve the prevention and treatment effect of the oral diseases is worthy of further research.

Disclosure of Invention

Aiming at the defects in the prior art, the invention provides an oral spray containing 10-HDA and a preparation method thereof. The oral spray prepared by 10-HDA and other important components can effectively inhibit bacterial microorganisms from breeding in the oral environment, protect the oral cavity to a great extent, improve the oral disease state and promote the normal repair of oral tissues, thereby maintaining the health of human bodies.

The technical scheme of the invention is as follows:

an oral spray containing 10-HDA is prepared from the following raw materials in parts by weight:

8-15 parts of mint, 8-15 parts of honeysuckle, 8-15 parts of dandelion, 8-15 parts of houttuynia cordata, 0.5-1.5 parts of sodium benzoate, 1-2 parts of 10-hydroxy-2-decenoic acid (10-HDA), 0.5-2 parts of menthol, 1-3 parts of vitamin C, 2-10 parts of cane sugar and 2500 parts of water 1500-.

According to the invention, the 10-HDA-containing oral spray is preferably prepared from the following raw materials in parts by weight:

10-12 parts of mint, 10-12 parts of honeysuckle, 10-12 parts of dandelion, 10-12 parts of houttuynia cordata, 0.8-1.0 part of sodium benzoate, 1.5-1.8 parts of 10-hydroxy-2-decenoic acid (10-HDA), 1-1.5 parts of menthol, 2-2.5 parts of vitamin C, 5-8 parts of sucrose and 2100 parts of water 1900 and sodium chloride.

According to the invention, the honeysuckle is known as a good medicine for clearing away heat and toxic materials from old times; has obvious bacteriostatic effect on bacillus subtilis, staphylococcus aureus, colibacillus, pseudomonas aeruginosa, etc. The dandelion has the effects of clearing heat and removing toxicity, relieving swelling and resolving masses, and inducing diuresis for treating stranguria, and is a good medicine for treating sore and pyogenic infections, pharyngalgia and stranguria caused by heat. Herba Houttuyniae has effects of eliminating phlegm, expelling pus, relieving pain, promoting urination, relieving swelling, and treating stranguria; the active ingredients contained in the antibacterial cream comprise decanoyl acetaldehyde, methyl n-nonanone, myrcene, sunflower aldehyde and the like, and have broad-spectrum antibacterial activity. The mint is beneficial to sweating and relieving heat, soothing the liver and regulating qi, relieving sore throat and pain and relieving itching, and phytochemicals such as pulegone, carvone, L-menthol, L-menthone and the like contained in the mint have antibacterial and anti-inflammatory effects. The traditional Chinese medicines supplement each other, and the broad-spectrum anti-inflammatory and bacteriostatic properties of the traditional Chinese medicine are effectively improved.

The sodium benzoate is a preservative for oral liquid medicament, and has the effects of preventing deterioration and souring and prolonging the shelf life. It is one of the important acid type food preservatives and is widely used in food processing and preservation. And can be used as food additive, mordant in dye industry, plasticizer in plastic industry, and intermediate for organic synthesis of perfume.

10-hydroxy-2-decenoic acid (10-HDA) is a specific high-efficiency bioactive substance of royal jelly and accounts for 1.4% -2.0% of fresh royal jelly. The 10-HDA has diverse physiological activities, including antifatigue, radioprotective, cardiovascular and cerebrovascular diseases treating, immunity regulating, memory improving, antiinflammatory, and cosmetic effect increasing as synergist. And can also inhibit gram-negative bacteria and gram-positive bacteria by interfering the adhesion of the cell surface, and has better inhibition effect on common bacteria such as escherichia coli, staphylococcus aureus and the like. In addition, 10-HDA has obvious effect of inhibiting the growth of cancer cells and enhancing the phagocytic function of phagocytic cells. Namely, 10-hydroxy-2-decenoic acid (10-HDA) has good bacteriostatic and cancer cell growth inhibiting effects.

Menthol, consisting of a saturated cyclic alcohol obtained from peppermint oil. Has anti-inflammatory and analgesic effects on sore throat and aphtha of the mouth and tongue. In addition, menthol has anti-irritant effect, and can promote the generation of new secretion from trachea, and discharge thick mucus easily, so it has expectorant and antitussive effects. Meanwhile, the addition of the menthol also has the function of improving the flavor of the substance.

Vitamin C can improve immunity, and has effects of treating scurvy and preventing microvascular rupture. It also has therapeutic effect on tooth atrophy and gingival hemorrhage.

Sucrose is an important sweetener and has the important functions of increasing the mouthfeel of food and regulating the flavor.

The preparation method of the oral spray containing 10-HDA comprises the following steps:

(1) soaking herba Menthae, flos Lonicerae, herba Taraxaci, and herba Houttuyniae in distilled water, decocting to boil, boiling for 15-20 min, and filtering to obtain first decoction and residue; adding distilled water into the filter residue, decocting until boiling, boiling for 15-20 minutes, and filtering to obtain a second decoction; mixing the first decoction and the second decoction, cooling, filtering, and concentrating to obtain mixed solution 1;

(2) fully mixing sodium benzoate, 10-hydroxy-2-decenoic acid (10-HDA), menthol, vitamin C and sucrose, and dispersing in distilled water to obtain mixed solution 2;

(3) and uniformly mixing the mixed solution 1 and the mixed solution 2, and sterilizing to obtain the oral spray containing 10-HDA.

Preferably, in step (1), the soaking time is 20 to 30 minutes.

Preferably, in step (1), the decoction method comprises the following steps: decocting with strong fire until boiling, and decocting with slow fire until boiling for 15-20 min.

According to the invention, in the step (1), the ratio of the total volume of the distilled water used for two times of decoction to the volume of the obtained mixed solution 1 is 2-4: 1.

According to the invention, the volume ratio of the distilled water used in the first decoction, the distilled water used in the second decoction and the distilled water used in the step (2) is preferably 4-8:3: 2.

Preferably, in step (3), pasteurization is carried out; sterilizing at 68-70 deg.C for 30 min.

The beneficial effects are as follows:

the invention reasonably improves the raw material components of the oral spray, and the oral spray is prepared by adopting 10-HDA and other important components, so that the use quality of the oral spray is well improved.

In the invention, 10-HDA is innovatively, reasonably and effectively added into the oral spray, so that the bacteriostatic effect of the oral spray can be effectively improved. The raw material 10-HDA is matched with other components to play a synergistic effect together, so that the obtained oral spray has the effects of diminishing inflammation, relieving swelling and pain, relieving tooth atrophy and gingival bleeding, inhibiting bacteria and resisting viruses, can effectively resist bacterial microorganisms from breeding in the oral environment, protects the oral cavity to a great extent, improves the disease state in the oral cavity, and promotes the normal repair of tissues in the oral cavity, thereby maintaining the health of human bodies. The oral spray disclosed by the invention can form a layer of protective film when sprayed in the oral cavity, so that damaged tissues are well protected, further damage of pathogenic factors is reduced, and the quick repair of the damaged tissues is promoted. Meanwhile, the oral spray also has good effects of clearing away heat and toxic materials, dispelling wind and heat, moistening lung to arrest cough, and removing toxic materials and swelling.

Detailed Description

The present invention is further illustrated by, but not limited to, the following examples.

Meanwhile, the experimental methods described in the following examples are all conventional methods unless otherwise specified; the reagents, materials and equipment are commercially available, unless otherwise specified.

Example 1

A method for preparing an oral spray containing 10-HDA comprises the steps of:

(1) putting 10g of honeysuckle, 10g of dandelion, 10g of houttuynia cordata and 10g of mint into a marmite, adding 1000ml of distilled water, and soaking for 20-30 minutes. After soaking, decocting with strong fire, and decocting with slow fire for 15-20 min after boiling. Filtering out the first decoction, adding 600ml distilled water into the filter residue, decocting with strong fire, boiling, decocting with slow fire for 15-20 min, and filtering out the second decoction; mixing the first and second filtrates, cooling, filtering, collecting filtrate, and concentrating the filtrate to 600 ml.

(2) 0.8g of sodium benzoate, 1.5g of 10-HDA, 2g of vitamin C, 1g of menthol and 5g of sucrose are dissolved in 400ml of sterile distilled water, and the mixture is stirred with a magnetic stirrer while being heated until the components are uniformly mixed.

(3) And (3) mixing the solutions obtained in the steps (1) and (2), and stirring by using a magnetic stirrer until the components are uniformly mixed.

(4) And (4) filling the mixed solution obtained in the step (3) into a cleaned plastic spray bottle, sealing the plastic spray bottle, and placing the plastic spray bottle into a water bath at the temperature of 68-70 ℃ for 30min for pasteurization to obtain the product.

Example 2

A method for preparing an oral spray containing 10-HDA comprises the steps of:

(2) Dissolving 1g of sodium benzoate, 1.8g of 10-HDA, 2.5g of vitamin C, 1.5g of menthol and 8g of sucrose in 400ml of sterile distilled water, and heating and stirring with a magnetic stirrer until the components are uniformly mixed.

Example 3

A method for preparing an oral spray containing 10-HDA comprises the steps of:

(1) putting 12g of honeysuckle, 12g of dandelion, 12g of houttuynia cordata and 12g of mint into a marmite, adding 1000ml of distilled water, and soaking for 20-30 minutes. After soaking, decocting with strong fire, and decocting with slow fire for 15-20 min after boiling. Filtering out the first decoction, adding 600ml distilled water into the filter residue, decocting with strong fire, boiling, decocting with slow fire for 15-20 min, and filtering out the second decoction; mixing the first and second filtrates, cooling, filtering, collecting filtrate, and concentrating the filtrate to 600 ml.

Comparative example 1

A process for the preparation of an oral spray, as described in example 1, except that: in the step (2), 10-HDA is not added; other conditions and procedures were consistent with example 1.

Comparative example 2

A process for the preparation of an oral spray, as described in example 1, except that: in the step (2), sodium benzoate is not added; other conditions and procedures were consistent with example 1.

Comparative example 3

A process for the preparation of an oral spray, as described in example 1, except that: in the step (1), honeysuckle is not added; other conditions and procedures were consistent with example 1.

Comparative example 4

A process for the preparation of an oral spray, as described in example 1, except that: in the step (1), dandelion is not added; other conditions and procedures were consistent with example 1.

Comparative example 5

A process for the preparation of an oral spray, as described in example 1, except that: in the step (1), the houttuynia cordata is not added; other conditions and procedures were consistent with example 1.

Comparative example 6

A process for the preparation of an oral spray, as described in example 1, except that: in the step (1), mint is not added; other conditions and procedures were consistent with example 1.

Test example 1

Culturing Staphylococcus aureus in LB culture medium (sodium chloride 10g/L, peptone 10g/L, yeast extract 5g/L) to logarithmic phase, and adjusting thallus concentration to 106/L with LB culture medium to obtain a bacterial liquid containing Staphylococcus aureus.

10ml of the bacterial suspension was taken, 10ml of the oral spray prepared in example 1 or comparative examples 1 to 6 was added thereto, and the mixture was cultured at 37 ℃ for 6 hours. The plate coating experiment was performed by sucking 200. mu.l, and the plate was left to stand overnight in an incubator for 12 hours, and then the growth of Staphylococcus aureus was observed, and the results are shown in Table 1 below.

Table 1 antibacterial performance test results of oral sprays of examples and comparative examples

Group of	Evaluation of inhibitory Effect against Staphylococcus aureus
		Example 1	+++++
Comparative example 1	+
		Comparative example 2	++
Comparative example 3	+++
		Comparative example 4	++++
Comparative example 5	++++
		Comparative example 6	+++

As can be seen from table 1, the oral spray of example 1 has a good bacteriostatic effect, and has a high inhibitory rate against staphylococcus aureus, a common bacterium.

Comparing example 1 with comparative example 1, it can be seen that when 10-HDA is not added, the inhibitory effect on Staphylococcus aureus is greatly reduced, which is far less than the bacteriostatic effect of example 1.

Comparing example 1 with comparative examples 1 and 2, it can be seen that the effect of inhibiting staphylococcus aureus was also reduced without adding sodium benzoate, but the effect was less significant than that of 10-HDA.

Comparing example 1 with comparative examples 3 and 6, it can be seen that honeysuckle and mint have certain promotion effects on inhibiting staphylococcus aureus, and the bacteriostatic effects of the honeysuckle and the mint are equivalent.

Comparing example 1 with comparative examples 4 and 5, it can be seen that dandelion and houttuynia cordata have certain inhibitory effect on staphylococcus aureus.

The raw materials of the oral spray provided by the invention play a synergistic effect, so that the oral spray provided by the invention has excellent antibacterial performance.

Test example 2

(1) The oral spray containing 10-HDA prepared in example 1 was subjected to taste evaluation, with the following steps:

a. all professional subjects tasted the same sample in equal amounts at the same time, placed in the mouth for approximately 10 seconds to provide time for evaluation, and then expectorated (swished) the sample;

b. recording initial taste and mouthfeel characteristics and aftertaste characteristics;

c. before repeating taste, subjects purified their taste buds with water and salt-free biscuit for 1, 3, 5, 10min

(2) Scoring criteria-intensity rating

0 ═ none

1 is mild

2 is moderate

3 is strong

(3) The results of the evaluations of different professional subjects were averaged for the same taste profile and the final results are listed in table 2 below:

TABLE 2 mouth spray mouth feel evaluation results

	Initiation of	1min	3min	5min	10min
						Sweet taste	3	2	1	0	0
Sour taste	0	0	0	0	0
						Bitter taste	1	1	0	0	0
Heating device	0	0	0	0	0
						Pricking pain of tongue	0	0	0	0	0
Numbness of limbs	0	0	0	0	0
						Drying	0	0	0	0	0
Other peculiar smell	1	1	0	0	0

The result shows that the oral spray prepared by the invention has good mouthfeel.

Claims

1. An oral spray containing 10-HDA is characterized by comprising the following raw materials in parts by weight:

2. The oral spray containing 10-HDA according to claim 1, which is prepared from the following raw materials in parts by weight:

3. A process for the preparation of a 10-HDA-containing oral spray according to any one of claims 1 or 2, comprising the steps of:

4. The method for preparing an oral spray containing 10-HDA according to claim 3, wherein the soaking time in step (1) is 20-30 minutes.

5. The method for preparing an oral spray containing 10-HDA according to claim 3, wherein the decoction in step (1) is performed by: decocting with strong fire until boiling, and decocting with slow fire until boiling for 15-20 min.

6. The method for preparing an oral spray containing 10-HDA according to claim 3, wherein in step (1), the ratio of the total volume of distilled water used for the two decocts to the volume of the obtained mixture 1 is 2-4: 1.

7. The method of claim 3, wherein the volume ratio of the distilled water used in the first decoction to the distilled water used in the second decoction to the distilled water used in step (2) is 4-8:3: 2.

8. The method for preparing an oral spray containing 10-HDA according to claim 3, wherein in step (3), pasteurization is performed; sterilizing at 68-70 deg.C for 30 min.