CN113575580A - Grape sunscald prevention and treatment agent - Google Patents
Grape sunscald prevention and treatment agent Download PDFInfo
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- CN113575580A CN113575580A CN202110914574.XA CN202110914574A CN113575580A CN 113575580 A CN113575580 A CN 113575580A CN 202110914574 A CN202110914574 A CN 202110914574A CN 113575580 A CN113575580 A CN 113575580A
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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Abstract
The invention discloses a grape sunscald control medicament, which comprises a blueberry extract, a wheat bran extract, propyl gallate and glycine; the preparation method of the blueberry extract comprises the following steps: cutting blueberry pulp into pieces, adding deionized water, extracting at constant temperature of 50-60 ℃, filtering, and concentrating under reduced pressure to obtain a blueberry extract; the preparation method of the wheat bran extract comprises the following steps: (1) washing wheat bran, soaking at normal temperature, drying, adding into hydrochloric acid solution, stirring, filtering, washing solid phase, and drying to obtain solid phase A; (2) preparing a sodium hydroxide solution, soaking the solid phase A in the sodium hydroxide solution, stirring the solution, adding hydrogen peroxide into the solution during stirring, continuously stirring, then filtering, adjusting the pH of the liquid phase to 4.0-4.5, concentrating under reduced pressure, precipitating with ethanol, collecting precipitate, washing the precipitate with absolute ethyl alcohol, and drying to obtain the wheat bran extract. The agent disclosed by the invention has a good effect of preventing and treating the sunscald of the grapes, and the sunscald incidence of the grapes is reduced.
Description
Technical Field
The invention belongs to the technical field of agricultural agents, and particularly relates to a grape sunscald control agent.
Background
The sunscald disease of grapes is also called as sunburn disease and is the sunburn injury caused by partial dehydration of fruit surface. The disease mostly occurs in the hard nucleus stage of berries. When the fruit is sunburned, the surface of the fruit generates light brown approximately round spots, the edge is not obvious, the surface of the fruit is shrunk and then gradually dented, and serious ears are changed into dry fruits. Tender top parts with curly hair and young shoots not yet lignified can also suffer from sunburn damage, resulting in wilting and browning of shoot tips or tender leaves. Sunscald usually occurs on ears exposed to sunlight. High temperature and strong light are main causes of sunburn, and especially sudden high temperature and strong light are more likely to cause sunburn. Besides high temperature and strong sunlight radiation, many other factors such as drought, variety sensitivity, rootstock and scion, row direction, tree structure and mineral nutrition all affect the incidence and severity of sunburn.
With global warming, extreme climatic events such as high temperature, drought and the like are increased in frequency and strength, and the sun burn of fruits increasingly becomes a prominent and serious problem in current agricultural production. With the increasing demand for high quality fruit consumption, the fruit industry is more and more competitive, and the overall quality of the fruit becomes more and more important. Finding a technology for effectively preventing and treating sunscald of grapes and simultaneously improving the yield and quality of fruits is one of the key points of current research and has important significance.
Disclosure of Invention
The invention provides a grape sunscald control medicament, which comprises a blueberry extract, a wheat bran extract, propyl gallate and glycine;
the preparation method of the blueberry extract comprises the following steps: cutting blueberry pulp into pieces, adding deionized water into the blueberry pieces, extracting at constant temperature of 50-60 ℃, filtering after extraction, and concentrating a liquid phase under reduced pressure to obtain the blueberry extract;
the preparation method of the wheat bran extract comprises the following steps:
(1) washing wheat bran with deionized water, soaking at normal temperature for more than 2h, drying, adding into hydrochloric acid solution, stirring for more than 8h, filtering, washing solid phase with deionized water, and drying to obtain solid phase A;
(2) preparing a sodium hydroxide solution, soaking the solid phase A in the sodium hydroxide solution, stirring the solution, adding hydrogen peroxide into the solution in the stirring process, continuously stirring for 2-3 hours, then filtering, adjusting the pH value of a liquid phase to 4.0-4.5, concentrating under reduced pressure, precipitating the concentrated solution with ethanol, collecting precipitates, washing the precipitates with absolute ethyl alcohol, and drying to obtain the wheat bran extract.
Further, in the sunscald control medicament, the components in parts by weight are as follows: 10 parts of blueberry extract, 2-6 parts of wheat bran extract, 1-2 parts of propyl gallate and 1-2 parts of glycine.
Further, the solid-liquid mass ratio of the blueberry pieces/the deionized water added into the blueberry pieces is 1: 7-8; extracting at the constant temperature of 50-60 ℃ for 5-7 h, and concentrating under reduced pressure to 1/4-1/3 of the volume before concentration to obtain the blueberry extract.
Further, the pH value of the hydrochloric acid solution is 3.0-3.5, and the mass of the added hydrochloric acid solution is 8-10 times of that of the wheat bran.
Further, in the sodium hydroxide solution, the concentration of sodium hydroxide is 0.4-0.5 mol/L, the balance is water, and the solid phase A is soaked in the sodium hydroxide solution at a solid-liquid mass ratio of 1: 5-6; the mass percentage of the solute of the hydrogen peroxide is 20%, and the mass of the added hydrogen peroxide is 1.5-1.8 times of the mass of the solid phase A.
The invention also discloses an application method of the grape sunscald control medicament, which is characterized in that the components of the grape sunscald control medicament are uniformly mixed and then divided into two parts, wherein one part is mixed with water to form a leaf spraying agent, the other part is mixed with a cerium ammonium nitrate aqueous solution to form a pouring liquid, the grape sunscald control medicament is applied to grapes in two months of 6 months and 7 months, wherein the application is carried out once every 3 days, the pouring liquid is used for pouring grapes fundamentally, and the leaf spraying agent is used for spraying grape leaves and fruits.
Further, when the leaf spray is configured, the weight ratio of the grape sunscald control agent to water is: water is 1: 200-300; when the pouring liquid is prepared, the grape sunscald control medicament and the ceric ammonium nitrate aqueous solution are in mass ratio to the grape sunscald control medicament: the mass percentage of the ammonium ceric nitrate in the aqueous solution of ammonium ceric nitrate is 4-5%.
Furthermore, the amount of the irrigation liquid is 4-5 jin/plant and the amount of the blade spraying agent is 10-12 jin/plant in each application.
The invention has the beneficial effects that: tests show that the sunscald control medicament has good control effect on the sunscald of grapes, reduces the sunscald incidence of grapes, and improves the quality of fruits. The reason for this is probably because the present invention can improve the total antioxidant capacity of grapes, increase the content of active substances in plant tissues, and further reduce the sunburn damage of plants.
Drawings
FIG. 1 is a graph comparing the incidence of sunscald on grapes after each example or comparative agent was applied using the application method described in example 5 or example 6, respectively.
Detailed Description
The following is a detailed description with reference to examples:
example 1
A grape sunscald preventing and treating agent comprises fructus Myrtilli extract, wheat bran extract, propyl gallate and glycine; the components are as follows by weight: 10 parts of blueberry extract, 2 parts of wheat bran extract, 1 part of propyl gallate and 1 part of glycine.
The preparation method of the blueberry extract comprises the following steps: cutting blueberry pulp into pieces, adding deionized water into the blueberry pieces according to the solid-liquid mass ratio of the blueberry pieces to the deionized water of 1:7, extracting at the constant temperature of 55 +/-5 ℃ for 5 hours, filtering after the extraction is finished, and concentrating a liquid phase under reduced pressure to 1/3 of the volume before concentration to obtain the blueberry extract;
the preparation method of the wheat bran extract comprises the following steps:
(1) washing the wheat bran with deionized water, adding the washed wheat bran into the deionized water according to the mass ratio of 1:10 of the wheat bran to the deionized water, soaking for 2 hours at normal temperature, drying, adding into a hydrochloric acid solution, wherein the pH value of the hydrochloric acid solution is 3.0, and the mass of the hydrochloric acid solution is 8 times of that of the wheat bran; stirring for 8h at the speed of 60r/min, then filtering, washing the solid phase with deionized water for 3 times, and drying at the temperature of 80 ℃ to obtain a solid phase A;
(2) preparing a sodium hydroxide solution, wherein the concentration of sodium hydroxide in the sodium hydroxide solution is 0.4mol/L, and the balance is water; soaking the solid phase A in the sodium hydroxide solution, wherein the solid-liquid mass ratio of the solid phase A to the liquid phase A is 1: 5; stirring the solution at the speed of 60r/min, and adding hydrogen peroxide into the solution in the stirring process, wherein the mass percentage of the solute of the hydrogen peroxide is 20%, and the adding mass of the hydrogen peroxide is 1.5 times of that of the solid phase A; stirring for 2 hours at the speed of 60r/min, filtering, adjusting the pH of a liquid phase to 4.0 by using a hydrochloric acid solution of 3mol/L, concentrating under reduced pressure to 1/2 of the volume before concentration, precipitating the concentrated solution by using an ethanol water solution with the volume fraction of 70%, collecting a precipitate, washing the precipitate for 3 times by using absolute ethanol, and drying at the temperature of 80 ℃ to obtain the wheat bran extract.
Example 2
A grape sunscald preventing and treating agent comprises fructus Myrtilli extract, wheat bran extract, propyl gallate and glycine; the components are as follows by weight: 10 parts of blueberry extract, 4 parts of wheat bran extract, 1 part of propyl gallate and 1 part of glycine.
The preparation method of the blueberry extract comprises the following steps: cutting blueberry pulp into pieces, adding deionized water into the blueberry pieces according to the solid-liquid mass ratio of the blueberry pieces to the deionized water of 1:7, extracting at the constant temperature of 55 +/-5 ℃ for 6 hours, filtering after the extraction is finished, and concentrating a liquid phase under reduced pressure to 1/3 of the volume before concentration to obtain the blueberry extract;
the preparation method of the wheat bran extract comprises the following steps:
(1) washing the wheat bran with deionized water, adding the washed wheat bran into the deionized water according to the mass ratio of 1:10 of the wheat bran to the deionized water, soaking for 2 hours at normal temperature, drying, adding into a hydrochloric acid solution, wherein the pH value of the hydrochloric acid solution is 3.0, and the mass of the hydrochloric acid solution is 8 times of that of the wheat bran; stirring for 8h at the speed of 60r/min, then filtering, washing the solid phase with deionized water for 3 times, and drying at the temperature of 80 ℃ to obtain a solid phase A;
(2) preparing a sodium hydroxide solution, wherein the concentration of sodium hydroxide in the sodium hydroxide solution is 0.4mol/L, and the balance is water; soaking the solid phase A in the sodium hydroxide solution, wherein the solid-liquid mass ratio of the solid phase A to the liquid phase A is 1: 5; stirring the solution at the speed of 60r/min, and adding hydrogen peroxide into the solution in the stirring process, wherein the mass percentage of the solute of the hydrogen peroxide is 20%, and the adding mass of the hydrogen peroxide is 1.6 times of that of the solid phase A; stirring for 2 hours at the speed of 60r/min, filtering, adjusting the pH of a liquid phase to 4.0 by using a hydrochloric acid solution of 3mol/L, concentrating under reduced pressure to 1/2 of the volume before concentration, precipitating the concentrated solution by using an ethanol water solution with the volume fraction of 70%, collecting a precipitate, washing the precipitate for 3 times by using absolute ethanol, and drying at the temperature of 80 ℃ to obtain the wheat bran extract.
Example 3
A grape sunscald preventing and treating agent comprises fructus Myrtilli extract, wheat bran extract, propyl gallate and glycine; the components are as follows by weight: 10 parts of blueberry extract, 5 parts of wheat bran extract, 2 parts of propyl gallate and 2 parts of glycine.
The preparation method of the blueberry extract comprises the following steps: cutting blueberry pulp into pieces, adding deionized water into the blueberry pieces according to the solid-liquid mass ratio of the blueberry pieces to the deionized water of 1:8, extracting at the constant temperature of 55 +/-5 ℃ for 6 hours, filtering after the extraction is finished, and concentrating a liquid phase under reduced pressure to 1/4 of the volume before concentration to obtain the blueberry extract;
the preparation method of the wheat bran extract comprises the following steps:
(1) washing the wheat bran with deionized water, adding the washed wheat bran into the deionized water according to the mass ratio of 1:10 of the wheat bran to the deionized water, soaking for 2 hours at normal temperature, drying, adding into a hydrochloric acid solution, wherein the pH value of the hydrochloric acid solution is 3.5, and the mass of the added hydrochloric acid solution is 8 times of that of the wheat bran; stirring for 8h at the speed of 60r/min, then filtering, washing the solid phase with deionized water for 3 times, and drying at the temperature of 80 ℃ to obtain a solid phase A;
(2) preparing a sodium hydroxide solution, wherein the concentration of sodium hydroxide in the sodium hydroxide solution is 0.5mol/L, and the balance is water; soaking the solid phase A in the sodium hydroxide solution, wherein the solid-liquid mass ratio of the solid phase A to the liquid phase A is 1: 6; stirring the solution at the speed of 60r/min, and adding hydrogen peroxide into the solution in the stirring process, wherein the mass percentage of the solute of the hydrogen peroxide is 20%, and the adding mass of the hydrogen peroxide is 1.7 times of that of the solid phase A; stirring for 3 hours at the speed of 60r/min, filtering, adjusting the pH of a liquid phase to 4.5 by using a hydrochloric acid solution of 3mol/L, concentrating under reduced pressure to 1/2 of the volume before concentration, precipitating the concentrated solution by using an ethanol water solution with the volume fraction of 70%, collecting a precipitate, washing the precipitate for 3 times by using absolute ethanol, and drying at the temperature of 80 ℃ to obtain the wheat bran extract.
Example 4
A grape sunscald preventing and treating agent comprises fructus Myrtilli extract, wheat bran extract, propyl gallate and glycine; the components are as follows by weight: 10 parts of blueberry extract, 6 parts of wheat bran extract, 2 parts of propyl gallate and 2 parts of glycine.
The preparation method of the blueberry extract comprises the following steps: cutting blueberry pulp into pieces, adding deionized water into the blueberry pieces according to the solid-liquid mass ratio of the blueberry pieces to the deionized water of 1:8, extracting at the constant temperature of 55 +/-5 ℃ for 7 hours, filtering after the extraction is finished, and concentrating a liquid phase under reduced pressure to 1/4 of the volume before concentration to obtain the blueberry extract;
the preparation method of the wheat bran extract comprises the following steps:
(1) washing the wheat bran with deionized water, adding the washed wheat bran into the deionized water according to the mass ratio of 1:10 of the wheat bran to the deionized water, soaking for 2 hours at normal temperature, drying, adding into a hydrochloric acid solution, wherein the pH value of the hydrochloric acid solution is 3.5, and the mass of the added hydrochloric acid solution is 8 times of that of the wheat bran; stirring for 8h at the speed of 60r/min, then filtering, washing the solid phase with deionized water for 3 times, and drying at the temperature of 80 ℃ to obtain a solid phase A;
(2) preparing a sodium hydroxide solution, wherein the concentration of sodium hydroxide in the sodium hydroxide solution is 0.5mol/L, and the balance is water; soaking the solid phase A in the sodium hydroxide solution, wherein the solid-liquid mass ratio of the solid phase A to the liquid phase A is 1: 6; stirring the solution at the speed of 60r/min, and adding hydrogen peroxide into the solution in the stirring process, wherein the mass percentage of the solute of the hydrogen peroxide is 20%, and the adding mass of the hydrogen peroxide is 1.8 times of that of the solid phase A; stirring for 3 hours at the speed of 60r/min, filtering, adjusting the pH of a liquid phase to 4.5 by using a hydrochloric acid solution of 3mol/L, concentrating under reduced pressure to 1/2 of the volume before concentration, precipitating the concentrated solution by using an ethanol water solution with the volume fraction of 70%, collecting a precipitate, washing the precipitate for 3 times by using absolute ethanol, and drying at the temperature of 80 ℃ to obtain the wheat bran extract.
Comparative example 1
A contrast agent comprising propyl gallate and glycine; the mass ratio of the propyl gallate to the glycine is 1: 1.
Comparative example 2
A contrast agent comprises blueberry extract, propyl gallate and glycine; the components are as follows by weight: 10 parts of blueberry extract, 1 part of propyl gallate and 1 part of glycine.
The preparation method of the blueberry extract comprises the following steps: cutting blueberry pulp into pieces, adding deionized water into the blueberry pieces according to the solid-liquid mass ratio of the blueberry pieces to the deionized water of 1:7, extracting at the constant temperature of 55 +/-5 ℃ for 6 hours, filtering after the extraction is finished, and concentrating a liquid phase under reduced pressure to 1/3 of the volume before concentration to obtain the blueberry extract;
comparative example 3
A contrast agent comprises wheat bran extract, propyl gallate and glycine; the components are as follows by weight: 4 parts of wheat bran extract, 1 part of propyl gallate and 1 part of glycine.
The preparation method of the wheat bran extract comprises the following steps:
(1) washing the wheat bran with deionized water, adding the washed wheat bran into the deionized water according to the mass ratio of 1:10 of the wheat bran to the deionized water, soaking for 2 hours at normal temperature, drying, adding into a hydrochloric acid solution, wherein the pH value of the hydrochloric acid solution is 3.0, and the mass of the hydrochloric acid solution is 8 times of that of the wheat bran; stirring for 8h at the speed of 60r/min, then filtering, washing the solid phase with deionized water for 3 times, and drying at the temperature of 80 ℃ to obtain a solid phase A;
(2) preparing a sodium hydroxide solution, wherein the concentration of sodium hydroxide in the sodium hydroxide solution is 0.4mol/L, and the balance is water; soaking the solid phase A in the sodium hydroxide solution, wherein the solid-liquid mass ratio of the solid phase A to the liquid phase A is 1: 5; stirring the solution at the speed of 60r/min, and adding hydrogen peroxide into the solution in the stirring process, wherein the mass percentage of the solute of the hydrogen peroxide is 20%, and the adding mass of the hydrogen peroxide is 1.6 times of that of the solid phase A; stirring for 2 hours at the speed of 60r/min, filtering, adjusting the pH of a liquid phase to 4.0 by using a hydrochloric acid solution of 3mol/L, concentrating under reduced pressure to 1/2 of the volume before concentration, precipitating the concentrated solution by using an ethanol water solution with the volume fraction of 70%, collecting a precipitate, washing the precipitate for 3 times by using absolute ethanol, and drying at the temperature of 80 ℃ to obtain the wheat bran extract.
Comparative example 4
A contrast agent comprises blueberry extract and wheat bran extract; the components are as follows by weight: 10 parts of blueberry extract and 4 parts of wheat bran extract.
The preparation method of the blueberry extract comprises the following steps: cutting blueberry pulp into pieces, adding deionized water into the blueberry pieces according to the solid-liquid mass ratio of the blueberry pieces to the deionized water of 1:7, extracting at the constant temperature of 55 +/-5 ℃ for 6 hours, filtering after the extraction is finished, and concentrating a liquid phase under reduced pressure to 1/3 of the volume before concentration to obtain the blueberry extract;
the preparation method of the wheat bran extract comprises the following steps:
(1) washing the wheat bran with deionized water, adding the washed wheat bran into the deionized water according to the mass ratio of 1:10 of the wheat bran to the deionized water, soaking for 2 hours at normal temperature, drying, adding into a hydrochloric acid solution, wherein the pH value of the hydrochloric acid solution is 3.0, and the mass of the hydrochloric acid solution is 8 times of that of the wheat bran; stirring for 8h at the speed of 60r/min, then filtering, washing the solid phase with deionized water for 3 times, and drying at the temperature of 80 ℃ to obtain a solid phase A;
(2) preparing a sodium hydroxide solution, wherein the concentration of sodium hydroxide in the sodium hydroxide solution is 0.4mol/L, and the balance is water; soaking the solid phase A in the sodium hydroxide solution, wherein the solid-liquid mass ratio of the solid phase A to the liquid phase A is 1: 5; stirring the solution at the speed of 60r/min, and adding hydrogen peroxide into the solution in the stirring process, wherein the mass percentage of the solute of the hydrogen peroxide is 20%, and the adding mass of the hydrogen peroxide is 1.6 times of that of the solid phase A; stirring for 2 hours at the speed of 60r/min, filtering, adjusting the pH of a liquid phase to 4.0 by using a hydrochloric acid solution of 3mol/L, concentrating under reduced pressure to 1/2 of the volume before concentration, precipitating the concentrated solution by using an ethanol water solution with the volume fraction of 70%, collecting a precipitate, washing the precipitate for 3 times by using absolute ethanol, and drying at the temperature of 80 ℃ to obtain the wheat bran extract.
Example 5
A grape sunscald prevention and control test is carried out in a planting garden in Tongguan county in Shaanxi with a Shaanxi grape variety 'Kyoho' as a research object. 7 independent grape sheds in the same garden are selected as test fields, and the grapes on each grape shed are independently applied with the agents prepared in the above examples or comparative examples, wherein the application method is as follows: the components of the agent are uniformly mixed and then divided into two parts, wherein one part is mixed with water to form a leaf spraying agent, the other part is mixed with a ceric ammonium nitrate aqueous solution to form a pouring liquid, the agent for preventing and treating the grape sunscald is applied to grapes in two months of 6 months and 7 months, wherein the pouring liquid is used for pouring the grapes fundamentally once every 3 days, the leaf spraying agent is used for spraying the grape leaves and fruits, the using amount of the pouring liquid is 4 jin/plant and the using amount of the leaf spraying agent is 10 jin/plant when each time of application is carried out. When the leaf spray is prepared, the grape sunscald control medicament and the water are in mass ratio to the grape sunscald control medicament: water 1: 250; when the pouring liquid is prepared, the grape sunscald control medicament and the ceric ammonium nitrate aqueous solution are in mass ratio to the grape sunscald control medicament: the mass percentage of the ammonium ceric nitrate in the aqueous solution of ammonium ceric nitrate is 4%. The rest field management is carried out according to the conventional method, and each group is the same. After harvesting the grapes, the incidence of sunscald of the grapes in each group of the grape shed was recorded, and the comparison results are shown in fig. 1.
Example 6
Taking Shaanxi grape variety 'Kyoho' as a research object, additionally selecting 7 independent grape sheds as test fields in the same garden where the experiment is carried out in example 5, and independently applying the medicaments prepared in the above examples or comparative examples to the grapes on each grape shed, wherein the application methods are as follows: the components of the agent are uniformly mixed, water is added to form a leaf spraying agent, the grape is applied for 6 months and 7 months, wherein the leaf spraying agent is applied once every 3 days, the leaf spraying agent is sprayed on the leaves and fruits of the grapes, and the dosage of the leaf spraying agent is 10 jin/plant when the grape is applied for each time. When the leaf spray is prepared, the grape sunscald control medicament and the water are in mass ratio to the grape sunscald control medicament: water 1: 250. The rest field management is carried out according to the conventional method, and each group is the same. After harvesting the grapes, the incidence of sunscald of the grapes in each group of the grape shed was recorded, and the comparison results are shown in fig. 1.
TABLE 1. incidence of fruit sunscald of grapes in each example or comparative example after application using the application method described in example 5 or example 6, respectively
As can be seen from Table 1, the sunscald control agent disclosed by the invention has a good control effect on grape sunscald, reduces the sunscald incidence of grapes, and improves the quality of fruits. The reason for this is probably because the present invention can improve the total antioxidant capacity of grapes, increase the content of active substances in plant tissues, and further reduce the sunburn damage of plants. As is clear from comparison between example 5 and example 6, the application scheme using spray + root application provided the sunscald control agent of the present invention exhibited significantly better effects than spray alone, probably due to the fact that the spray + root application combined with the application provided the oxidation resistance of the plant tissue was more significantly improved.
The technical solutions provided by the present invention are described in detail above, and for those skilled in the art, the ideas according to the embodiments of the present invention may be changed in the specific implementation manners and the application ranges, and in summary, the content of the present description should not be construed as limiting the present invention.
Claims (8)
1. A grape sunscald prevention and treatment medicament is characterized by comprising blueberry extract, wheat bran extract, propyl gallate and glycine;
the preparation method of the blueberry extract comprises the following steps: cutting blueberry pulp into pieces, adding deionized water into the blueberry pieces, extracting at constant temperature of 50-60 ℃, filtering after extraction, and concentrating a liquid phase under reduced pressure to obtain the blueberry extract;
the preparation method of the wheat bran extract comprises the following steps:
(1) washing wheat bran with deionized water, soaking at normal temperature for more than 2h, drying, adding into hydrochloric acid solution, stirring for more than 8h, filtering, washing solid phase with deionized water, and drying to obtain solid phase A;
(2) preparing a sodium hydroxide solution, soaking the solid phase A in the sodium hydroxide solution, stirring the solution, adding hydrogen peroxide into the solution in the stirring process, continuously stirring for 2-3 hours, then filtering, adjusting the pH value of a liquid phase to 4.0-4.5, concentrating under reduced pressure, precipitating the concentrated solution with ethanol, collecting precipitates, washing the precipitates with absolute ethyl alcohol, and drying to obtain the wheat bran extract.
2. The grape sunscald control agent as claimed in claim 1, wherein the sunscald control agent comprises the following components in parts by weight: 10 parts of blueberry extract, 2-6 parts of wheat bran extract, 1-2 parts of propyl gallate and 1-2 parts of glycine.
3. The grape sunscald control agent as claimed in claim 2, wherein the solid-liquid mass ratio of blueberry pieces/deionized water added to the blueberry pieces is 1: 7-8; extracting at the constant temperature of 50-60 ℃ for 5-7 h, and concentrating under reduced pressure to 1/4-1/3 of the volume before concentration to obtain the blueberry extract.
4. The agent for preventing and treating sunscald of grapes according to claim 2, wherein the pH of the hydrochloric acid solution is 3.0-3.5, and the mass of the hydrochloric acid solution added is 8-10 times of that of wheat bran.
5. The agent for preventing and treating sunscald of grapes according to claim 2, wherein the concentration of sodium hydroxide in the sodium hydroxide solution is 0.4-0.5 mol/L, the balance is water, and the solid-liquid mass ratio of the solid phase A soaked in the sodium hydroxide solution is 1: 5-6; the mass percentage of the solute of the hydrogen peroxide is 20%, and the mass of the added hydrogen peroxide is 1.5-1.8 times of the mass of the solid phase A.
6. The method of applying the agent for controlling sunscald of grapes according to any one of claims 1 to 5, wherein the components of the agent for controlling sunscald of grapes are mixed uniformly and then divided into two parts, one part is mixed with water to form a leaf spray, the other part is mixed with an aqueous solution of cerium ammonium nitrate to form a irrigation liquid, and the agent for controlling sunscald of grapes is applied to grapes in two months of 6 and 7 months, wherein the irrigation liquid is applied once every 3 days for irrigating grapes at all, and the leaf spray is applied for spraying grape leaves and fruits.
7. The method of applying a grape sunscald control agent according to claim 6, wherein the mass ratio of the grape sunscald control agent and water when the leaf spray is configured is:
water is 1: 200-300; when the pouring liquid is prepared, the grape sunscald control medicament and the ceric ammonium nitrate aqueous solution are in mass ratio to the grape sunscald control medicament: the mass percentage of the ammonium ceric nitrate in the aqueous solution of ammonium ceric nitrate is 4-5%.
8. The application method of the grape sunscald control agent according to claim 6, wherein the amount of the irrigation liquid is 4-5 jin/L and the amount of the leaf spraying agent is 10-12 jin/L in each application.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102812958A (en) * | 2012-09-03 | 2012-12-12 | 张军防 | Plant sunscald controlling agent |
| CN105028041A (en) * | 2015-06-30 | 2015-11-11 | 云南天质网络科技有限公司 | Prevention and control method for grape sunscald |
| CN109892330A (en) * | 2017-12-08 | 2019-06-18 | 中国科学院大连化学物理研究所 | A kind of crops sunscald control agent and its application |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102812958A (en) * | 2012-09-03 | 2012-12-12 | 张军防 | Plant sunscald controlling agent |
| CN105028041A (en) * | 2015-06-30 | 2015-11-11 | 云南天质网络科技有限公司 | Prevention and control method for grape sunscald |
| CN109892330A (en) * | 2017-12-08 | 2019-06-18 | 中国科学院大连化学物理研究所 | A kind of crops sunscald control agent and its application |
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