CN113575473A - Indoor seawater culture method - Google Patents
Indoor seawater culture method Download PDFInfo
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- CN113575473A CN113575473A CN202110979079.7A CN202110979079A CN113575473A CN 113575473 A CN113575473 A CN 113575473A CN 202110979079 A CN202110979079 A CN 202110979079A CN 113575473 A CN113575473 A CN 113575473A
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- chloride
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- algae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G33/00—Cultivation of seaweed or algae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention provides a seawater indoor culture method, which comprises the following steps: (1) constructing a water circulation system formed by connecting an algae culture pond, a microorganism purification pond and a fish pond in series; (2) injecting artificial seawater into the algae culture pond, the microorganism purification pond and the fish pond; (3) periodically putting the algae nutrient into the algae culture pond, and putting the microorganism nutrient into the microorganism purification pond. The indoor seawater culture method has the beneficial effects that an ecological system formed by mutually coupling an algae culture pond, a microorganism purification pond and a fish pond is constructed, wherein seawater algae is cultured in the algae culture pond, probiotics are cultured in the microorganism purification pond, seawater aquatic animals are cultured in the fish pond, artificial seawater is injected, and nutrient elements required by the algae and the microorganisms are periodically added into the system, so that the microorganisms and the algae can efficiently convert and absorb toxic wastes such as ammonia nitrogen and the like discharged by the aquatic animals in water, and meanwhile, the system has the characteristics of no water change and high-density culture.
Description
Technical Field
The invention relates to the technical field of cultivation, in particular to a seawater indoor cultivation method.
Background
With the increase of the demand of human marine products, marine fishing cannot meet the demands of people, and the damage of marine fishing to the marine ecosystem, the country is gradually tightening the restriction on marine fishing, so that the development of mariculture is a future trend. The history of mariculture in China has been long, traditional mariculture is developed in coastal areas, marine culture is carried out by building net cages on offshore places, but in recent years, due to pollution of human activities to offshore sea areas and pollution of seawater by offshore mariculture, the culture environment is deteriorated, and the ever-increasing marine needs of human beings are difficult to meet, so that the development of inland mariculture systems is very urgent. At present, the existing inland mariculture system has two modes of a seawater circulating aquaculture system and a cultivation symbiotic mariculture system, the seawater circulating aquaculture system relies on expensive purification equipment to purify seawater, the complete utilization of water resources is difficult to realize, and a part of water body needs to be replaced periodically to maintain the excellent water quality. The seawater cultivation symbiotic system does not need expensive purification equipment, inland seawater cultivation is realized by constructing an ecological system in which an artificial wetland, a microbial purification tank and a fish pond are mutually coupled, salt-tolerant plants are planted in the artificial wetland, a large amount of beneficial microbes are cultured in the microbial purification tank, and biological purification is carried out by means of the natural characteristics of the microbes and the salt-tolerant plants. However, the natural seawater is deficient in nutrients required by plants and microorganisms, and is difficult to purify waste such as ammonia nitrogen and the like discharged by fishes and shrimps in small water bodies at high efficiency, so that the cultivation density is low, meanwhile, various inorganic salt components in the natural seawater can interfere the detection of trace nutrient elements required by the plants and the microorganisms, and the content of the trace nutrient elements in the water bodies cannot be evaluated, so that the trace nutrient elements are difficult to detect and add continuously, and the speed of absorbing nitrate and phosphate by higher plants is not as low as that of algae. This patent proposes an inland fish algae intergrowth mariculture system, compares with present cultivation intergrowth mariculture system, and the biggest characteristics lie in: (1) the artificial seawater with a formula different from natural seawater is selected, so that the detection of the micronutrient elements required by the algae and microorganisms in the water body can be realized; (2) the nutrient solution required by algae and microorganisms in the inland fish-algae symbiotic mariculture system is constructed, and the adding method is explored, so that the following effects can be achieved: (1) completely avoid changing water and inland mariculture; (2) high density inland mariculture (>10kg/m 3).
Disclosure of Invention
The invention overcomes the defects in the prior art, provides a seawater indoor culture method, and can realize the aims of inland mariculture without water replacement and high-density inland mariculture.
In order to solve the technical problems, the invention is realized by the following technical scheme:
the indoor seawater culture method is characterized by comprising the following steps: the method comprises the following steps:
(1) constructing a water circulation system formed by connecting an algae culture pond, a microorganism purification pond and a fish pond in series;
(2) injecting artificial seawater into the algae culture pond, the microorganism purification pond and the fish pond;
(3) periodically putting the algae nutrient into the algae culture pond, and putting the microorganism nutrient into the microorganism purification pond.
Preferably, the artificial seawater comprises 2-45 g/L of sodium chloride.
Preferably, the artificial seawater comprises 2-45 g/L of sodium chloride, 1.2-2.2 g/L of potassium chloride, 0.8-1.3 g/L of calcium chloride, 0.02-0.45 g/L of magnesium chloride and 0.16-0.23 g/L of magnesium sulfate.
Preferably, the artificial seawater comprises 2-45 g/L of sodium chloride, 1.2-2.2 g/L of potassium chloride, 0.8-1.3 g/L of calcium chloride, 0.02-0.45 g/L of magnesium chloride, 0.03-0.35 g/L of magnesium chloride, 0.12-0.16 g/L of potassium sulfate, 0.08-0.12 g/L of magnesium sulfate, 0.15-0.22 g/L of sodium bicarbonate, 0.04-0.09 g/L of calcium hydroxide, 0.06-0.12 g/L of monopotassium phosphate, 0.02-0.25 g/L of dipotassium hydrogen phosphate, 0.12-0.15 g/L of sodium dihydrogen phosphate, 0.19-0.26 g/L of disodium hydrogen phosphate, 0.08-0.15 g/L of iron chloride, 0.05-0.09 g/L of zinc sulfate, 0.16-0.25 g/L of zinc chloride, 0.00000.01-0.04 g/L of zinc sulfate, 0.0001-0.01-0.0001 g/L of ammonium molybdate, 0.04-0.12 g/L of copper sulfate, 0.09-0.15 g/L boric acid and 0.16-0.23 g/L vitamin.
Preferably, the algae nutrient comprises 0.05-0.7 g/L calcium chloride and 0.01-0.5 g/L magnesium sulfate.
Preferably, the algae nutrient comprises 0.05-0.7 g/L of calcium chloride, 0.01-0.5 g/L of magnesium sulfate, 0.005-0.02 g/L of ferric chloride, 0.001-0.015 g/L of zinc sulfate, 0.00001-0.0001 g/L of copper sulfate, 0.0016-0.0025 g/L of manganese sulfate, 0.0011-0.0025 g/L of ammonium molybdate and 0.001-0.005 g/L of boric acid.
Preferably, the algae nutritional agent comprises 0.05-0.7 g/L of calcium chloride, 0.01-0.5 g/L of magnesium sulfate, 0.005-0.02 g/L of ferric chloride, 0.001-0.015 g/L of zinc sulfate, 0.00001-0.0001 g/L of copper sulfate, 0.c,
0.0016-0.0025 g/L manganese sulfate, 0.0011-0.0025 g/L ammonium molybdate, 0.001-0.005 g/L boric acid, 0.001-0.005 g/L magnesium chloride, 0.003-0.012 g/L potassium sulfate, 0.005-0.016 g/L magnesium sulfate,
0.0008-0.0012 g/L monopotassium phosphate, 0.0001-0.0005 g/L dipotassium phosphate, 0.0016-0.0025 g/L ferric chloride, 0.0025-0.0035 g/L zinc sulfate, 0.0011-0.0025 g/L zinc chloride, 0.00001-0.0001 g/L copper sulfate, 0.0005-0.0016 g/L manganese sulfate, 0.0003-0.0015 g/L ammonium molybdate, 0.03-0.06 g/L boric acid and 0.019-0.028 g/L vitamin.
Preferably, the microbial nutrient comprises sodium bicarbonate with the addition amount of 0.01-0.5 g/L.
Preferably, the microbial nutrient comprises 0.01-0.5 g/L of sodium bicarbonate, 0.00001-0.0001 g/L of copper sulfate, 0.002-0.06 g/L of manganese sulfate and 0.011-0.03 g/L of vitamin.
Preferably, the microbial nutrient comprises 0.01-0.5 g/L of sodium bicarbonate, 0.011-0.03 g/L of potassium chloride, 0.015-0.036 g/L of calcium chloride, 0.022-0.034 g/L of magnesium chloride, 0.015-0.023 g/L of potassium sulfate, and,
0.001 to 0.02g/L magnesium sulfate, 0.014 to 0.024g/L sodium bicarbonate, 0.012 to 0.023g/L calcium hydroxide,
0.011 to 0.03g/L potassium bicarbonate, 0.016 to 0.024g/L potassium dihydrogen phosphate, 0.019 to 0.023g/L dipotassium hydrogen phosphate,
0.02-0.06 g/L sodium dihydrogen phosphate, 0.015-0.022 g/L disodium hydrogen phosphate, 0.05-0.11 g/L ferric chloride, 0.019-0.028 g/L zinc sulfate, 0.005-0.008 g/L zinc chloride, 0.00001-0.0001 g/L copper sulfate, 0.033-0.045 g/L manganese sulfate, 0.024-0.036 g/L ammonium molybdate, 0.028-0.039 g/L boric acid and 0.011-0.03 g/L vitamin.
Compared with the prior art, the invention has the beneficial effects that:
the method comprises the steps of constructing an ecological system formed by mutually coupling an algae culture pond, a microorganism purification pond and a fish pond, culturing seawater algae in the algae culture pond, culturing probiotics in the microorganism purification pond, culturing seawater aquatic animals in the fish pond, injecting artificial seawater with components different from natural seawater, and periodically adding an algae nutrient and a microorganism nutrient to the system, so that the microorganisms and the algae can efficiently convert and absorb toxic wastes such as ammonia nitrogen and the like discharged by the aquatic animals in water, and meanwhile, the system has the characteristics of no water change and high-density culture.
Detailed Description
A seawater indoor culture method is characterized in that: the method comprises the following steps:
(1) constructing a water circulation system formed by connecting an algae culture pond, a microorganism purification pond and a fish pond in series;
(2) injecting artificial seawater into the algae culture pond, the microorganism purification pond and the fish pond;
(3) periodically putting the algae nutrient into the algae culture pond, and putting the microorganism nutrient into the microorganism purification pond.
The artificial seawater comprises sodium chloride with the addition amount of 30 g/L.
As a further preferable mode, the artificial seawater comprises sodium chloride in an amount of 30g/L, potassium chloride in an amount of 1.8g/L, calcium chloride in an amount of 0.9g/L, magnesium chloride in an amount of 0.3g/L and magnesium sulfate in an amount of 0.19 g/L.
As a further preferable mode, the artificial seawater comprises sodium chloride added in an amount of 30g/L, potassium chloride added in an amount of 1.8g/L, calcium chloride added in an amount of 0.9g/L, magnesium chloride added in an amount of 0.3g/L, magnesium chloride added in an amount of 0.2g/L, potassium sulfate added in an amount of 0.15g/L, magnesium sulfate added in an amount of 0.1g/L, sodium hydrogen carbonate added in an amount of 0.18g/L, calcium hydroxide added in an amount of 0.06g/L, potassium dihydrogen phosphate added in an amount of 0.09g/L, dipotassium hydrogen phosphate added in an amount of 0.3g/L, sodium dihydrogen phosphate added in an amount of 0.14g/L, disodium hydrogen phosphate added in an amount of 0.22g/L, ferric chloride added in an amount of 0.12g/L, zinc sulfate added in an amount of 0.07g/L, zinc chloride added in an amount of 0.18g/L, copper sulfate added in an amount of 0.00008g/L, manganese sulfate added in an amount of 0.05g/L, ammonium molybdate added in an amount of 0.09g/L, boric acid added in an amount of 0.12g/L, and vitamins added in an amount of 0.1 g/L.
The algae nutrient comprises 0.2g/L calcium chloride and 0.015g/L magnesium sulfate.
As a further preferable mode, the algae nutrient includes calcium chloride in an amount of 0.02g/L, magnesium sulfate in an amount of 0.015g/L, ferric chloride in an amount of 0.009g/L, zinc sulfate in an amount of 0.0013g/L, copper sulfate in an amount of 0.00008g/L, manganese sulfate in an amount of 0.0018g/L, ammonium molybdate in an amount of 0.0019g/L, and boric acid in an amount of 0.004 g/L.
As a further preferable mode, the algae nutrient comprises calcium chloride in an amount of 0.02g/L, magnesium sulfate in an amount of 0.015g/L, ferric chloride in an amount of 0.015g/L, zinc sulfate in an amount of 0.0013g/L, copper sulfate in an amount of 0.00008g/L, manganese sulfate in an amount of 0.0018g/L, ammonium molybdate in an amount of 0.0018g/L, boric acid in an amount of 0.004g/L, magnesium chloride in an amount of 0.004g/L, potassium sulfate in an amount of 0.011g/L, 0.008g/L magnesium sulfate, 0.001g/L potassium dihydrogen phosphate, 0.0004g/L dipotassium hydrogen phosphate, 0.0018g/L ferric chloride, 0.0028g/L zinc sulfate, 0.0016g/L zinc chloride, 0.00008g/L copper sulfate, 0.0012g/L manganese sulfate, 0.0012g/L ammonium molybdate, 0.05g/L boric acid and 0.022g/L vitamin.
The microbial nutrient comprises sodium bicarbonate with an addition amount of 0.02 g/L.
As a further preferable scheme, the microbial nutrient comprises sodium bicarbonate with the addition amount of 0.02g/L, copper sulfate with the addition amount of 0.00008g/L, manganese sulfate with the addition amount of 0.03g/L and vitamin with the addition amount of 0.015 g/L.
As a further preferable mode, the microbial nutrient comprises sodium bicarbonate with the addition amount of 0.02g/L, potassium chloride with the addition amount of 0.02g/L, calcium chloride with the addition amount of 0.018g/L, magnesium chloride with the addition amount of 0.03g/L, potassium sulfate with the addition amount of 0.018g/L, magnesium sulfate with the addition amount of 0.015g/L, sodium bicarbonate with the addition amount of 0.018g/L, calcium hydroxide with the addition amount of 0.018g/L, potassium bicarbonate with the addition amount of 0.02g/L, monopotassium phosphate with the addition amount of 0.02g/L, 0.02g/L dipotassium hydrogen phosphate, 0.04g/L sodium dihydrogen phosphate, 0.018g/L disodium hydrogen phosphate, 0.08g/L ferric chloride, 0.022g/L zinc sulfate, 0.006g/L zinc chloride, 0.00008g/L copper sulfate, 0.038g/L manganese sulfate, 0.026g/L ammonium molybdate, 0.03g/L boric acid and 0.02g/L vitamin.
In practical application, firstly, a water circulation system with an algae culture pond, a microorganism purification pond and a fish pond connected in series is constructed, 1000L of water and 30kg of sodium chloride are added into the system, and the water circulation system and the sodium chloride are uniformly mixed to form artificial seawater; then respectively putting 200g of calcium chloride and 150g of magnesium sulfate into the algae culture pond to culture marine algae, putting 200g of sodium bicarbonate into the microorganism purification pond to culture probiotics, culturing marine aquatic animals in the fish pond, and then supplementing the calcium chloride, the magnesium sulfate and the sodium bicarbonate according to the calcium magnesium concentration and the bicarbonate concentration of the water body every day, thereby realizing the high-efficiency conversion and absorption of the microorganisms and the algae on toxic wastes such as ammonia nitrogen and the like discharged by the aquatic animals in the water.
Finally, it should be noted that: although the present invention has been described in detail with reference to the embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.
Claims (10)
1. The indoor seawater culture method is characterized by comprising the following steps: the method comprises the following steps:
(1) constructing a water circulation system formed by connecting an algae culture pond, a microorganism purification pond and a fish pond in series;
(2) injecting artificial seawater into the algae culture pond, the microorganism purification pond and the fish pond;
(3) periodically putting the algae nutrient into the algae culture pond, and putting the microorganism nutrient into the microorganism purification pond.
2. The seawater indoor culture method according to claim 1, wherein: the artificial seawater comprises 2-45 g/L of sodium chloride.
3. The seawater indoor culture method according to claim 1, wherein: the artificial seawater comprises 2-45 g/L of sodium chloride, 1.2-2.2 g/L of potassium chloride, 0.8-1.3 g/L of calcium chloride, 0.02-0.45 g/L of magnesium chloride and 0.16-0.23 g/L of magnesium sulfate.
4. The seawater indoor culture method according to claim 1, wherein: the artificial seawater comprises 2-45 g/L of sodium chloride, 1.2-2.2 g/L of potassium chloride, 0.8-1.3 g/L of calcium chloride, 0.02-0.45 g/L of magnesium chloride, 0.03-0.35 g/L of magnesium chloride, 0.12-0.16 g/L of potassium sulfate, 0.08-0.12 g/L of magnesium sulfate, 0.15-0.22 g/L of sodium bicarbonate, 0.04-0.09 g/L of calcium hydroxide and 0.06-0.12 g/L of monopotassium phosphate, 0.02-0.25 g/L dipotassium hydrogen phosphate, 0.12-0.15 g/L sodium dihydrogen phosphate, 0.19-0.26 g/L disodium hydrogen phosphate, 0.08-0.15 g/L ferric chloride, 0.05-0.09 g/L zinc sulfate, 0.16-0.25 g/L zinc chloride, 0.00001-0.0001 g/L copper sulfate, 0.01-0.09 g/L manganese sulfate, 0.04-0.12 g/L ammonium molybdate, 0.09-0.15 g/L boric acid and 0.16-0.23 g/L vitamin.
5. The seawater indoor culture method according to claim 1, wherein: the algae nutrient comprises 0.05-0.7 g/L of calcium chloride and 0.01-0.5 g/L of magnesium sulfate.
6. The seawater indoor culture method according to claim 1, wherein: the algae nutrient agent comprises 0.05-0.7 g/L of calcium chloride, 0.01-0.5 g/L of magnesium sulfate, 0.005-0.02 g/L of ferric chloride, 0.001-0.015 g/L of zinc sulfate, 0.00001-0.0001 g/L of copper sulfate, 0.0016-0.0025 g/L of manganese sulfate, 0.0011-0.0025 g/L of ammonium molybdate and 0.001-0.005 g/L of boric acid.
7. The seawater indoor culture method according to claim 1, wherein: the algae nutrient comprises 0.05-0.7 g/L calcium chloride, 0.01-0.5 g/L magnesium sulfate, 0.005-0.02 g/L ferric chloride, 0.001-0.015 g/L zinc sulfate, 0.00001-0.0001 g/L copper sulfate, 0.0016-0.0025 g/L manganese sulfate, 0.0011-0.0025 g/L ammonium molybdate, 0.001-0.005 g/L boric acid, 0.001-0.005 g/L magnesium chloride and 0.003-0.012 g/L potassium sulfate, 0.005-0.016 g/L magnesium sulfate, 0.0008-0.0012 g/L potassium dihydrogen phosphate, 0.0001-0.0005 g/L dipotassium hydrogen phosphate, 0.0016-0.0025 g/L ferric chloride, 0.0025-0.0035 g/L zinc sulfate, 0.0011-0.0025 g/L zinc chloride, 0.0005-0.0016 g/L manganese sulfate, 0.0003-0.0015 g/L ammonium molybdate, 0.03-0.06 g/L boric acid and 0.019-0.028 g/L vitamin.
8. The seawater indoor culture method according to claim 1, wherein: the microbial nutrient comprises 0.01-0.5 g/L of sodium bicarbonate.
9. The seawater indoor culture method according to claim 1, wherein: the microbial nutrient comprises 0.01-0.5 g/L of sodium bicarbonate, 0.00001-0.0001 g/L of copper sulfate, 0.002-0.06 g/L of manganese sulfate and 0.011-0.03 g/L of vitamin.
10. The seawater indoor culture method according to claim 1, wherein: the microbial nutrient comprises 0.01-0.5 g/L sodium bicarbonate, 0.011-0.03 g/L potassium chloride, 0.015-0.036 g/L calcium chloride, 0.022-0.034 g/L magnesium chloride, 0.015-0.023 g/L potassium sulfate, 0.001-0.02 g/L magnesium sulfate, 0.014-0.024 g/L sodium bicarbonate, 0.012-0.023 g/L calcium hydroxide, 0.011-0.03 g/L potassium bicarbonate, 0.016-0.024 g/L potassium dihydrogen phosphate, 0.019-0.023 g/L dipotassium hydrogen phosphate, 0.02-0.06 g/L sodium dihydrogen phosphate, 0.015-0.033 g/L disodium hydrogen phosphate, 0.05-0.11 g/L ferric chloride, 0.019-0.019 g/L ferric sulfate, 0.022-0.028 g/L zinc sulfate, 0.022-0.022 g/L zinc sulfate, 0.024-0.036 g/L of ammonium molybdate, 0.028-0.039 g/L of boric acid and 0.011-0.03 g/L of vitamin.
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