CN113559188A

CN113559188A - Traditional Chinese medicine composition for improving pelvic inflammation and preparation method thereof

Info

Publication number: CN113559188A
Application number: CN202111065119.3A
Authority: CN
Inventors: 吕向东
Original assignee: Zhejiang Yanghe Health Management Technology Co ltd
Current assignee: Zhejiang Yanghe Health Management Technology Co ltd
Priority date: 2021-09-11
Filing date: 2021-09-11
Publication date: 2021-10-29

Abstract

The invention provides a traditional Chinese medicine composition for improving pelvic inflammation and a preparation method thereof, and relates to the technical field of medicine research and development. The traditional Chinese medicine composition comprises: caulis aristolochiae, szechwan chinaberry fruit, codonopsis pilosula, Chinese violet, rhizoma arisaematis, talcum powder, combined spicebush root, earthworm, myrrh, rhizoma corydalis and gentian. The Chinese medicinal composition has the effects of promoting qi circulation, promoting blood circulation, dispelling pathogenic wind, removing dampness, resolving hard mass and relieving pain, and can be used for effectively treating qi stagnation type pelvic inflammatory disease. The preparation method of the traditional Chinese medicine composition disclosed by the invention adopts the combination of microwave extraction and supercritical extraction, so that the effective components of the raw materials can be better extracted, and the medicinal effect of the medicine is better.

Description

Traditional Chinese medicine composition for improving pelvic inflammation and preparation method thereof

Technical Field

The invention relates to the technical field of medicine research and development, and particularly relates to a traditional Chinese medicine composition for improving pelvic inflammation and a preparation method thereof.

Background

Pelvic inflammation refers to inflammation of female reproductive organs, connective tissue around uterus, and pelvic peritoneum. Pelvic inflammatory disease refers to a group of infectious diseases of female upper genital tract, mainly including endometritis, tubal pregnancy and chronic pelvic pain, and the inflammation is recurrent, which seriously affects the reproductive health of women. Pelvic inflammation can be divided into acute and chronic according to the pathogenesis process and clinical manifestations, and is mainly characterized in that: lower abdominal pain, leukorrhagia, menoxenia, infertility, soreness of waist and limbs. Patients with severe pelvic inflammation can develop chronic peritonitis, septicemia and even toxic shock, if the acute stage is not completely cured, the patients turn to chronic pelvic inflammation, the chronic pelvic inflammation is difficult to cure, the harm to the patients is stronger, the chronic pelvic inflammation is common clinically at present, and the incidence rate, the course of disease is long, the condition of the disease is lingering, and the recurrence rate is high.

At present, the conventional treatment means is intervention treatment through medicines, but the curative effect of the existing medicine treatment is not obvious.

Disclosure of Invention

The invention aims to provide a traditional Chinese medicine composition for improving pelvic inflammation, which has the effects of promoting qi and blood circulation, dispelling wind and removing dampness, and resolving hard mass and relieving pain, and can effectively treat qi stagnation type pelvic inflammation.

The invention also aims to provide a preparation method of the traditional Chinese medicine composition for improving pelvic inflammatory disease, which combines microwave extraction and supercritical extraction to better extract effective components of raw materials, so that the medicinal effect of the medicine is better.

The technical problem to be solved by the invention is realized by adopting the following technical scheme.

The invention provides a traditional Chinese medicine composition for improving pelvic inflammation, which comprises the following raw materials in parts by weight: 5-10 parts of herba aristolochiae, 7-20 parts of szechwan chinaberry fruit, 10-20 parts of codonopsis pilosula, 3-8 parts of Chinese violet, 4-9 parts of rhizoma arisaematis, 1-5 parts of talcum powder, 2-6 parts of radix linderae, 3-7 parts of lumbricus, 2-7 parts of myrrh, 4-10 parts of rhizoma corydalis and 1-3 parts of gentian.

The invention provides a preparation method of a traditional Chinese medicine composition for improving pelvic inflammation, which comprises the following steps:

grinding caulis Aristolochiae, fructus Toosendan, radix Codonopsis, herba Violae, rhizoma arisaematis, radix Linderae, Lumbricus, Myrrha, rhizoma corydalis, and radix Gentianae, adding ethanol into the obtained powder, microwave extracting at 30-40 deg.C for 20-40min, filtering to obtain extractive solution and residue, and supercritical CO extracting the residue₂Fluid extraction is carried out for 15-25min to obtain extract, the extract and the extract are mixed and then concentrated for 0.5-1h under-0.006 to-0.008 MPa, the obtained extract is dried for 10-20min at 50-60 ℃ to obtain powder, and then talcum powder and the powder are mixed.

The traditional Chinese medicine composition for improving pelvic inflammation and the preparation method thereof provided by the embodiment of the invention have at least the following beneficial effects:

the toosendan fruit has the effects of promoting qi circulation and relieving pain, the total alkaloid in the corydalis tuber has a good pain relieving effect, after the toosendan fruit and the corydalis tuber are used in a compatible mode, the effects of promoting qi circulation and activating blood circulation are synergistic, the pain relieving effects are mutually promoted, pelvic inflammation lower abdominal pain is effectively relieved or treated, the qi circulation in vivo can be helped to be smooth, and the leucorrhea is recovered to be normal. In addition, the szechwan chinaberry fruit, the codonopsis pilosula and the combined spicebush root can further enhance the effect of promoting qi circulation, and the codonopsis pilosula also has the effect of tonifying middle-jiao, can nourish the body while promoting qi circulation, avoids body weakness and weakens the effect of promoting qi circulation. The toosendan fruit, the corydalis tuber and the henbane stem are used together, and the pain relieving effect is further enhanced by the compatibility of the toosendan fruit, the corydalis tuber and the henbane stem, so that the lower abdominal pain or the waist and limb pain can be effectively treated. The Chinese violet has the functions of clearing away heat and toxic materials and promoting diuresis, the talc has the functions of clearing away heat and promoting diuresis, and the earthworm has the functions of clearing away heat and promoting diuresis, and the three are used together to achieve the synergistic effect of clearing away heat and promoting diuresis, so that the inflammation of a body is weakened, and the qi circulation and pain relieving effects are stronger when the qi circulation and pain relieving medicines are matched. The caulis aristolochiae and the rhizoma arisaematis are used in combination, have the synergistic effects of dredging collaterals and dissipating stagnation, and are matched with qi-moving medicines, so that channels and collaterals in a body can move qi smoothly, further pain is weakened, leucorrhea is recovered to be normal, and menstruation is recovered to be normal. In the embodiment, the raw materials are matched with each other, and the medicine has better effects of promoting qi circulation, relieving pain, dredging collaterals, resolving masses, clearing heat and promoting diuresis under a proper proportion, so that the medicine has a better treatment effect on qi stagnation type pelvic inflammation.

The preparation method of the traditional Chinese medicine composition adopts the combination of microwave extraction and supercritical extraction, can better extract the effective components of the raw materials, and the effective components are hardly lost in the extraction process, so that the final medicine has high content of the effective components and good quality, and the medicine has better treatment effect on qi stagnation type pelvic inflammation.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to specific examples.

A traditional Chinese medicine composition for improving pelvic inflammation comprises the following raw materials in parts by weight: 5-10 parts of herba aristolochiae, 7-20 parts of szechwan chinaberry fruit, 10-20 parts of codonopsis pilosula, 3-8 parts of Chinese violet, 4-9 parts of rhizoma arisaematis, 1-5 parts of talcum powder, 2-6 parts of radix linderae, 3-7 parts of lumbricus, 2-7 parts of myrrh, 4-10 parts of rhizoma corydalis and 1-3 parts of gentian.

Tianxian Teng, bitter in taste and warm in nature, entering liver, spleen and kidney meridians, has the actions of promoting qi and blood circulation, and activating meridians to stop pain, and is mainly indicated for abdominal stabbing pain and wind-damp arthralgia. Caulis Aristolochiae mainly contains magnoline, cyclanoline, aristolochic acid, and beta-sitosterol. Toosendan fruit, bitter and cold in flavor, enters liver, small intestine and bladder channels, has the effects of soothing liver, clearing heat, promoting qi circulation, relieving pain and killing parasites, and is used for treating liver depression, fire, distending pain of chest, hypochondrium and stomach, hernia pain, parasitic infestation and abdominal pain. The fructus Toosendan mainly contains toosendanin, alkaloid, kaempferol, resin, and tannin. Chuan Lian Zi has the actions of dispersing liver heat and relieving pain, and is often combined with Yan Hu Suo to achieve the synergistic effect of qi-moving and blood-activating, so as to treat abdominal pain and hypochondriac pain caused by stagnation of liver qi and excessive liver-gallbladder fire. Chuan Lian Zi is the essential herb for hernia, and is combined with Wu Yao to promote the circulation of qi.

Radix Codonopsis, radix Codonopsis is sweet in taste and neutral in nature, has effects of invigorating spleen and replenishing qi, invigorating spleen and benefiting lung, resisting cancer, lowering blood pressure, resisting anoxia, resisting aging, enhancing immunity, improving activity of superoxide dismutase, enhancing ability of eliminating free radicals, regulating gastrointestinal motility, resisting ulcer, inhibiting gastric acid secretion, and reducing pepsin activity, and is suitable for spleen and lung weakness, short breath, cardiopalmus, anorexia, loose stool, ulcer, anemia, asthma cough, internal heat, diabetes, etc. The effective components of radix Codonopsis have exciting effect on nervous system, and can enhance organism resistance, increase erythrocyte and hemoglobin, and zinc can promote growth and development and improve sexual function. The Chinese violet has bitter and pungent taste and cold property, enters heart and liver channels, has the functions of clearing heat and detoxicating, cooling blood and detumescence, clearing heat and promoting diuresis, has the function of resisting pathogenic microorganisms, and has the inhibiting effect on pseudomonas aeruginosa, escherichia coli, typhoid bacillus and dysentery bacillus.

Tian nan xing is bitter and pungent in flavor and warm in nature, enters lung, liver and spleen channels, has the efficacy of resolving masses and relieving swelling, and is externally used for treating carbuncle swelling and snake and insect bites. The rhizoma arisaematis has the effects of eliminating dampness and phlegm, dispelling wind and relieving spasm, and resolving masses and eliminating swelling. The rhizoma arisaematis contains saponin and mannitol, and the two substances have good effects of reducing phlegm and dispelling pathogenic wind. The product mainly contains various alkaloids and cyclic dipeptide compounds, beta-sitosterol, various amino acids and trace elements, and also has effects of relieving convulsion, tranquilizing, relieving pain, eliminating phlegm, resisting tumor and oxidation, agglutinating erythrocyte and inhibiting Hela cell growth; it excels at dispelling wind-phlegm from meridians and collaterals, resolving masses and relieving swelling (abscess, deep-rooted carbuncle and swelling pain syndrome) and is indicated for abscess, deep-rooted carbuncle and swelling pain.

The talcum has the effects of promoting urination, treating stranguria, clearing away summer-heat, astringing dampness and healing sore, promoting urination, excreting dampness, clearing away heat, and relieving damp-heat along with urination; the main component is hydrous magnesium silicate, and also contains clay and lime. Talcum powder can protect inflamed gastrointestinal tract mucous membrane, prevent vomiting and diarrhea, prevent poison from being absorbed in gastrointestinal tract, and inhibit typhoid bacillus, paratyphoid bacillus and meningococcus. Wherein the magnesium silicate has adsorption and astringency effects, and can protect intestinal canal.

Radix Linderae, pungent and warm, and entering lung, spleen, kidney and bladder channels, has effects of activating qi-flowing, relieving pain, warming kidney and dispelling cold, and can be used for treating cold coagulation and qi stagnation, chest and abdomen distending pain, adverse qi, dyspnea with urgency, bladder deficiency cold, enuresis, frequent micturition, hernia pain, and cold channel abdominal pain; mainly contains alkaloid, lindera root lactone, new lindera root lactone, lindera root alkene, alpha-curcumene, lindera root ether, and isosafrole. The specific action mechanism is as follows: relieving exterior syndrome and regulating muscles, and relieving epigastric distention and qi; the cold-dispelling effect can be used to remove the cold from the passenger; pathogenic qi is dispelled by the attack of Tianmianza; relieving stagnation of qi, abdominal pain, distending pain of chest and diaphragm, which can be reduced immediately; it is also indicated for wind-stroke with obstruction of limbs due to the action of qi and stamina. The combined spicebush root has stronger analgesic and anti-inflammatory effects, has the strongest analgesic and anti-inflammatory activity of the n-butyl alcohol part, and has the inhibition effect on staphylococcus aureus, streptococcus hemolyticus A, typhoid bacillus, proteus bacillus, pseudomonas aeruginosa and escherichia coli.

Lumbricus, has effects of clearing heat, arresting convulsion, dredging collaterals, relieving asthma, and promoting diuresis, and can be used for treating hyperpyrexia, unconsciousness, convulsive epilepsy, convulsion, arthralgia, cough and asthma due to lung heat, oliguria, edema, and hypertension. Earthworm contains many kinds of amino acids, and has the actions of relieving diarrhea and sexual disorder due to its salty cold, extinguishing wind and relieving spasm, and clearing and resolving high fever. Earthworm is good at passing through meridians and collaterals, is used for the syndrome of blocked meridians and collaterals, unsmooth blood vessels and adverse limb joints caused by various reasons, and is combined with qi-tonifying and blood-moving herbs to treat qi deficiency, blood stasis and adverse meridians and collaterals.

Myrrh, pungent and bitter in flavor, neutral in nature, enters heart, liver and spleen channels, removes stasis and relieves pain, and promotes blood circulation and removes blood stasis. Myrrha mainly contains Myrrha resin, gum, bisaboloid, formic acid, acetic acid and oxidase; the volatile oil contains eugenol, m-methyl phenol, pinene, limonene, cinnamaldehyde, etc., and has effects of protecting liver, reducing blood lipid, relieving pain, resisting tumor, resisting bacteria, resisting inflammation, inhibiting contraction of uterine smooth muscle, breaking blood, dispelling blood stasis, and relieving swelling and pain. It is combined with qi-moving and stasis-resolving herbs to promote blood circulation and stop bleeding. Preparing talc: clearing heat and relieving swelling. Myrrh is pungent and bitter in flavor and has the effects of removing blood stasis and relieving swelling; talcum is sweet in taste, bland in flavor, cold in nature, and capable of clearing heat and excreting dampness. The two medicines are combined to eliminate damp-heat, eliminate swelling, remove blood stasis and clear heat. Myrrh compatibility with corydalis tuber: myrrha promotes blood circulation to stop pain, and rhizoma corydalis promotes blood circulation to stop pain and promotes qi circulation, both of them have effects of promoting blood circulation, promoting qi circulation and relieving pain, and can be used for treating abdominal pain and dysmenorrhea due to blood stasis and qi stagnation.

Corydalis tuber, pungent, bitter and warm, enters liver and spleen channels, has the effects of activating blood, promoting qi circulation and relieving pain, and is used for treating chest and hypochondrium, epigastric and abdominal pain, chest obstruction and cardialgia, amenorrhea and dysmenorrheal, postpartum stasis and swelling pain. Corydalis tuber contains more than 20 kinds of alkaloids, such as corydaline, isocorydine, berberine, cryptopine, dehydronanine, dihydrosanguinarine, tetrahydroberberine, protopine, alpha-allocryptopine, coptisine, palmatine, etc. The total alkali contains tetrahydropalmatine, linoleic acid, vanillic acid, daucosterol, dehydrocorydaline and the like, and the analgesic effect of corydaline, corydaline and corydaline is most obvious, wherein the corydaline is the strongest, the corydaline is the second lowest, and the corydaline is the second highest.

Radix Gentianae, with cold nature and bitter taste, enters liver meridian and gallbladder meridian, has effects of clearing liver fire, eliminating dampness and heat, and invigorating stomach, and can be used for treating conjunctival congestion and dizziness, deafness and ear swelling, hypochondriac pain and bitter taste, laryngopharynx swelling and pain, epilepsy, convulsion, damp heat, suppurative sore, eczema, pudendal swelling, pudendal pruritus, stranguria with urine, and anorexia. Radix Gentianae has pharmacological effects of protecting liver, promoting gallbladder function, invigorating stomach, resisting inflammation, enhancing immunity, resisting hyperthyroidism, resisting bacteria, relieving pain, and tranquilizing mind. The radix Gentianae mainly contains gentiopicroside, swertiamarin, gentianine C, kaempferol, swertiamarin, trace amount of swertiamarin, gentisic and gentisic. The gentian has a protective effect on the damage of the carbon tetrachloride to the liver, can reduce the degeneration and necrosis of liver histiocytes, and has the effect of reducing transaminase; can contract gallbladder and increase bile secretion to promote gallbladder function. Gentiopicroside can promote the secretion of gastric juice and gastric acid in a small amount, and has the function of invigorating stomach; the large amount of the medicine taken can inhibit secretion, reduce appetite and obstruct digestion. The gentisic has significant anti-inflammatory effect. Gentiana scabra Bunge is a pure yin-nourishing drug, is healthy when used less, and is injured when used more, in particular, it can brighten the acquired fire of spleen and stomach and consume the innate fire of liver and kidney when it is dark.

In the embodiment, the szechwan chinaberry fruit has the effects of promoting qi circulation and relieving pain, the total alkali in the corydalis tuber has a good pain relieving effect, after the szechwan chinaberry fruit and the corydalis tuber are used in a matching way, the effects of promoting qi circulation and activating blood circulation are synergistic, the pain relieving effects are mutually promoted, pelvic inflammation lower abdominal pain is effectively relieved or treated, the qi circulation in a body can be promoted smoothly, and the leucorrhea is recovered to be normal. In addition, the szechwan chinaberry fruit, the codonopsis pilosula and the combined spicebush root can further enhance the effect of promoting qi circulation, and the codonopsis pilosula also has the effect of tonifying middle-jiao, can nourish the body while promoting qi circulation, avoids body weakness and weakens the effect of promoting qi circulation. The toosendan fruit, the corydalis tuber and the henbane stem are used together, and the pain relieving effect is further enhanced by the compatibility of the toosendan fruit, the corydalis tuber and the henbane stem, so that the lower abdominal pain or the waist and limb pain can be effectively treated. The Chinese violet has the functions of clearing away heat and toxic materials and promoting diuresis, the talc has the functions of clearing away heat and promoting diuresis, and the earthworm has the functions of clearing away heat and promoting diuresis, and the three are used together to achieve the synergistic effect of clearing away heat and promoting diuresis, so that the inflammation of a body is weakened, and the qi circulation and pain relieving effects are stronger when the qi circulation and pain relieving medicines are matched. The caulis aristolochiae and the rhizoma arisaematis are used in combination, have the synergistic effects of dredging collaterals and dissipating stagnation, and are matched with qi-moving medicines, so that channels and collaterals in a body can move qi smoothly, further pain is weakened, leucorrhea is recovered to be normal, and menstruation is recovered to be normal. In the embodiment, the raw materials are matched with each other, and the medicine has better effects of promoting qi circulation, relieving pain, dredging collaterals, resolving masses, clearing heat and promoting diuresis under a proper proportion, so that the medicine has a better treatment effect on qi stagnation type pelvic inflammation.

In the embodiment, the traditional Chinese medicine composition also comprises 10-25 parts of folium isatidis, 2-5 parts of tetrandra root, 1-5 parts of garden balsam seed, 3-7 parts of sculellaria barbata, 4-8 parts of rhizoma drynariae, 2-7 parts of radix clematidis and 1-6 parts of cornu bubali by weight.

Folium isatidis is bitter in taste and cold in nature, enters liver, heart, stomach and spleen channels, and has the effects of clearing heat and removing toxicity, cooling blood and removing ecchymoses. The dyers woad leaf has strong inhibiting effect on staphylococcus aureus, staphylococcus albus, streptococcus A and meningococcus; it also has certain inhibiting effect on Diplococcus pneumoniae, Bacillus influenzae and Bacillus dysenteriae. Folium Isatidis (Isatis tinctoria L.) has also significant anti-inflammatory effect. Scutellaria barbata, pungent and bitter in flavor and cold in nature, enters lung, liver and kidney meridians, and has the effects of clearing away heat and toxic materials, removing blood stasis and inducing diuresis. It mainly contains alkaloid, flavonoid glycoside, phenols and steroids, and has inhibitory effect on Staphylococcus aureus, Shigella flexneri, Salmonella typhi, Pseudomonas aeruginosa and Escherichia coli.

Tetrandra root has the effects of expelling wind-damp, relieving pain and inducing diuresis, has a certain analgesic effect, but the analgesic effect is weakened after the dosage is increased, also has the effects of relieving heat, and has weak relieving heat, also has a certain anti-inflammatory effect, has an obvious diuretic effect and can increase the urine volume by 47 percent. Acute seed, warm in nature, slightly bitter and pungent in flavor, enters lung channel and liver channel, and has the effects of breaking blood and softening hard masses, removing blood stasis and descending qi, and softening hard masses and dissipating stagnation. The garden balsam seed mainly contains impatiens sterol, saponin, flavone, stearidonic acid, b-balsamic alcohol, impatiens sterol, ethyl oleate, linoleic acid and the like, and has an inhibiting effect on staphylococcus aureus, hemolytic streptococcus, pseudomonas aeruginosa, typhoid bacillus. The rhizoma drynariae is warm in nature and bitter in taste, enters liver channels and kidney channels, has the effects of healing injury, relieving pain, tonifying kidney and strengthening bone, can promote the absorption of bone on calcium and improve the level of blood calcium and blood phosphorus, and the dihydroflavone of the rhizoma drynariae has the effects of easing pain and tranquilizing.

Clematis chinensis, warm in nature, pungent and salty in flavor, enters bladder meridian, has the effects of dispelling wind and removing dampness, dredging collaterals and relieving pain, and also has the effects of relieving pain, benefiting gallbladder, inducing labor, resisting microorganisms, resisting diuresis, reducing blood pressure and the like. The buffalo horn is cold in nature and bitter in taste, enters heart channel and liver channel, and has the effects of clearing heat and removing toxicity, cooling blood and arresting convulsion. The cornu bubali mainly contains various amino acids such as aspartic acid, threonine, serine, glutamic acid and the like, and contains various trace elements such as iron, zinc, copper, manganese and the like. It has tranquilizing and anticonvulsive effects; can enhance cardiac function, slow down myocardial contraction force and regulate blood pressure; has antiinflammatory and antiinfectious effects; can protect liver function.

In the embodiment, the folium isatidis, the sculellaria barbata, the tetrandra root, the garden balsam seed, the rhizoma drynariae, the radix clematidis and the buffalo horn are used as auxiliary materials, the folium isatidis, the sculellaria barbata and the buffalo horn have the effects of clearing away heat and toxic materials and promoting diuresis, and the heat clearing effect of the medicine is enhanced by matching with the raw materials, so that internal inflammation is weakened. The tetrandra root and clematis root have the functions of dispelling wind, removing dampness and relieving pain, and the effect of promoting the circulation of qi and removing blood stasis of the medicine is better when the tetrandra root and the clematis root are matched with the raw materials. Secondly, the dyers woad leaf, the barbed skullcap herb and the garden balsam seed are matched with the combined spicebush root, the talcum powder and the philippine violet herb, so that the inhibition effect of the medicament on bacteria is stronger, the body inflammation is obviously reduced, and the treatment effect of the medicament on the qi stagnation type pelvic inflammation is better.

Carotene, vitamins, a-protein, amino acids, calcium stearate, and cyclodextrin may also be included in this embodiment. The components can provide nutrient components for a body, improve the state of serious lack of partial nutrient components of the body caused by pelvic inflammation, nourish the body while treating the pelvic inflammation, fully nourish the body while recovering the normal symptoms, and further enhance the effect of treating the pelvic inflammation.

A preparation method of a traditional Chinese medicine composition for improving pelvic inflammation comprises the following steps:

caulis Aristolochiae, fructus Toosendan, radix Codonopsis, herba Violae, rhizoma arisaematis, radix Linderae, Lumbricus, Myrrha, and rhizoma corydalisGrinding radix Gentianae and Soochow gentian, sieving with 70-100 mesh sieve, soaking in ethanol for 20-30min, microwave extracting at 30-40 deg.C for 20-40min, filtering to obtain extractive solution and residue, and supercritical CO extracting the residue₂Fluid extraction is carried out for 15-25min to obtain extract, the extract and the extract are mixed and then concentrated for 0.5-1h under-0.006 to-0.008 MPa, the obtained extract is dried for 10-20min at 50-60 ℃ to obtain powder, and then talcum powder and the powder are mixed.

In this embodiment, grind the raw materials except that talcum powder and smash, extract after smashing and can accelerate active ingredient to appear in the medicinal material, add ethanol with the powder and soak 20-30min, can let ethanol soak the medicinal material earlier like this, when carrying out the microwave extraction, through the speed of motion of intensification acceleration ethanol molecule, let ethanol can better permeate the medicinal material more in more rapidly, and then extract the active ingredient of medicinal material. Extracting at 30-40 deg.C to avoid ethanol volatilization, and increase the molecular movement speed of ethanol and extraction speed of effective components; the extraction is carried out for 20-40min, so that the extraction effect is better, the extraction time is shortened, and the medicine preparation efficiency is further improved. Then the filter residue is treated by supercritical CO₂And (3) fluid extraction, which is to further extract the residual effective components in the filter residue by different extraction modes, so as to ensure the complete extraction of the effective components in the medicinal materials. The concentration of the effective components can be improved by mixing the extracting solution and then carrying out reduced pressure concentration, and the concentration is carried out under the pressure of-0.006 to-0.008 MPa, so that the loss of the effective components can be avoided, and the concentration efficiency is improved. Drying the extract into powder after concentration, specifically selecting spray drying, wherein the spray drying has good drying effect and better dryness of the powder.

Optionally, the mass ratio of powder to ethanol is 1: (1-10), the effective components in the powder have better precipitation effect under proper proportion, and further the effective components of the medicinal materials have better extraction effect. Wherein the volume fraction of ethanol is 70-80%, and the concentration of ethanol molecules is more suitable for extraction at the volume fraction.

Optionally, during microwave extraction, 2 times of extraction are performed, wherein the first time of extraction is performed at 30-40 deg.C for 30-40min, and the second time of extraction is performed at 40-50 deg.C for 10-20 min. Through twice extraction, extract under different temperatures, can extract the active ingredient of easy extraction earlier for the first time, let ethanol molecule rate of motion accelerate through the intensification for the second time, promote the speed of dissolving out and permeating of ethanol molecule in the medicinal material, and then let the active ingredient that is difficult to extract in the medicinal material propose out.

Optionally, after mixing the extract and the extraction liquid, and before concentrating, the method further comprises centrifuging the mixture, and concentrating the supernatant. Through centrifugal treatment, the residue in the extract can be separated, the content of the active ingredients of the medicine is improved after concentration, the residue is prevented from being mixed and reduced, and the medicine effect is further weakened. Centrifuging at 500-600r/min for 5-10min, under the condition, the residue is separated without loss of effective components.

Optionally, when the talcum powder and the material powder are mixed, stirring at 100-150r/min for 10-20min, and adding honey after mixing to prepare the water-honeyed pill. The uniformity of the medicinal powder can be improved by stirring, so that the treatment effect is better.

The features and properties of the present invention are described in further detail below with reference to examples.

Example 1

A traditional Chinese medicine composition for improving pelvic inflammation comprises the following raw materials:

5g of herba aristolochiae, 7g of szechwan chinaberry fruit, 10g of codonopsis pilosula, 3g of Chinese violet, 4g of rhizoma arisaematis, 1g of talcum powder, 2g of combined spicebush root, 3g of earthworm, 2g of myrrh, 4g of corydalis tuber and 1g of gentian.

grinding caulis Aristolochiae, fructus Toosendan, radix Codonopsis, herba Violae, rhizoma arisaematis, radix Linderae, Lumbricus, Myrrha, rhizoma corydalis, and radix Gentianae, pulverizing, sieving with 70 mesh sieve, adding ethanol into the powder, soaking for 20min, microwave extracting at 30 deg.C for 20min, filtering to obtain extractive solution and residue, and supercritical CO extracting the residue₂Extracting fluid for 15min to obtain extractive solution, mixing the extractive solutions, concentrating under-0.006 MPa for 0.5h, drying the obtained extract at 50 deg.C for 10min to obtain powder, and dryingMixing the talcum powder and the powder.

The mass ratio of the powder to the ethanol is 1: 1, the volume fraction of ethanol is 70%.

Example 2

10g of herba aristolochiae, 20g of szechwan chinaberry fruit, 20g of codonopsis pilosula, 8g of Chinese violet, 9g of rhizoma arisaematis, 5g of talcum powder, 6g of combined spicebush root, 7g of earthworm, 7g of myrrh, 10g of corydalis tuber and 3g of gentian.

grinding caulis Aristolochiae, fructus Toosendan, radix Codonopsis, herba Violae, rhizoma arisaematis, radix Linderae, Lumbricus, Myrrha, rhizoma corydalis, and radix Gentianae, pulverizing, sieving with 100 mesh sieve, adding ethanol into the powder, soaking for 30min, microwave extracting at 40 deg.C for 40min, filtering to obtain extractive solution and residue, and supercritical CO extracting the residue₂Extracting with fluid for 25min to obtain extractive solution, mixing the extractive solutions, concentrating under-0.008 MPa for 1h, drying the obtained extract at 60 deg.C for 20min to obtain powder, and mixing pulvis Talci with the powder.

The mass ratio of the powder to the ethanol is 1: 10, the volume fraction of ethanol is 80%.

Example 3

8g of herba aristolochiae, 10g of szechwan chinaberry fruit, 15g of codonopsis pilosula, 5g of philippine violet herb, 6g of arisaema consanguineum, 2g of talcum powder, 4g of combined spicebush root, 5g of earthworm, 3g of myrrh, 6g of corydalis tuber and 2g of gentian.

grinding caulis Aristolochiae, fructus Toosendan, radix Codonopsis, herba Violae, rhizoma arisaematis, radix Linderae, Lumbricus, Myrrha, rhizoma corydalis, and radix Gentianae, pulverizing, sieving with 80 mesh sieve, adding ethanol into the powder, soaking for 25min, microwave extracting at 35 deg.C for 30min, filtering to obtain extractive solution and residue, and supercritical CO extracting the residue₂Fluid extracting for 20min to obtain extractive solution, and extractingMixing the extractive solution and extractive solution, concentrating under-0.007 MPa for 0.7h, drying the obtained extract at 55 deg.C for 15min to obtain powder, and mixing pulvis Talci with the powder.

The mass ratio of the powder to the ethanol is 1: and 5, the volume fraction of the ethanol is 75 percent.

Example 4

7g of herba aristolochiae, 12g of szechwan chinaberry fruit, 18g of codonopsis pilosula, 4g of Chinese violet, 5g of arisaema consanguineum schott, 3g of talcum powder, 4g of radix linderae, 5g of earthworm, 4g of myrrh, 7g of rhizoma corydalis, 2g of gentian, 15g of folium isatidis, 4g of tetrandra root, 2g of garden balsam seed, 5g of barbed skullcap herb, 5g of rhizoma drynariae, 4g of radix clematidis and 2g of buffalo horn.

grinding caulis Aristolochiae, fructus Toosendan, radix Codonopsis, herba Violae, rhizoma arisaematis, radix Linderae, Lumbricus, Myrrha, rhizoma corydalis, and radix Gentianae, pulverizing, sieving with 80 mesh sieve, adding ethanol into the powder, soaking for 25min, microwave extracting at 35 deg.C for 30min, filtering to obtain extractive solution and residue, and supercritical CO extracting the residue₂Extracting with fluid for 20min to obtain extractive solution, decocting folium Isatidis, radix Stephaniae Tetrandrae, semen Impatientis, herba Scutellariae Barbatae, rhizoma Drynariae, radix Clematidis and cornu Bubali with water at 55 deg.C for 1.5 hr to obtain decoction;

mixing the extractive solution, the decoction and the extractive solution, concentrating under-0.007 MPa for 0.7h, drying the obtained extract at 55 deg.C for 15min to obtain powder, and mixing pulvis Talci with the powder.

The mass ratio of the powder to the ethanol is 1: and 6, the volume fraction of the ethanol is 75 percent.

Example 5

7g of herba aristolochiae, 12g of szechwan chinaberry fruit, 18g of codonopsis pilosula, 4g of Chinese violet, 5g of arisaema consanguineum schott, 3g of talcum powder, 4g of radix linderae, 5g of earthworm, 4g of myrrh, 7g of rhizoma corydalis, 2g of gentian, 15g of folium isatidis, 4g of tetrandra root, 2g of garden balsam seed, 5g of barbed skullcap herb, 5g of rhizoma drynariae, 4g of radix clematidis, 2g of buffalo horn, 5g of carotene, 10g of vitamin, 15g a-protein, 10g of amino acid, 2g of calcium stearate and 8g of cyclodextrin.

mixing the extractive solution, the decoction and the extractive solution, concentrating under-0.007 MPa for 0.7h, drying the obtained extract at 55 deg.C for 15min to obtain powder, mixing pulvis Talci with the powder, adding carotene, vitamins, a-protein, amino acids, calcium stearate and cyclodextrin, and mixing.

Example 6

grinding caulis Aristolochiae, fructus Toosendan, radix Codonopsis, herba Violae, rhizoma arisaematis, radix Linderae, Lumbricus, Myrrha, rhizoma corydalis, and radix Gentianae, pulverizing, sieving with 80 mesh sieve, adding ethanol, soaking for 25min, microwave extracting for 2 times, extracting for the first timeExtracting at 35 deg.C for 35min, extracting at 45 deg.C for 15min for 2 times, filtering to obtain extractive solution and residue, and extracting the residue with supercritical CO₂Extracting with fluid for 20min to obtain extractive solution, decocting folium Isatidis, radix Stephaniae Tetrandrae, semen Impatientis, herba Scutellariae Barbatae, rhizoma Drynariae, radix Clematidis and cornu Bubali with water at 55 deg.C for 1.5 hr to obtain decoction;

mixing the extractive solution, the decoction and the extractive solution, centrifuging at 550r/min for 8min, collecting supernatant, concentrating at-0.007 MPa for 0.8h, drying the obtained extract at 55 deg.C for 15min to obtain powder, mixing pulvis Talci with the powder, adding carotene, vitamins, a-protein, amino acids, calcium stearate and cyclodextrin, stirring at 120r/min for 15min, mixing, adding Mel, and making into water-honeyed pill.

1. Toxicology test

1) Acute toxicity test: selecting 60 healthy mice, dividing into 6 groups, and sequentially administrating the traditional Chinese medicine composition of examples 1-7 of the invention to each group, specifically, adding 2mL of water into 10g of the traditional Chinese medicine composition to dissolve the traditional Chinese medicine composition to obtain a liquid medicine, and administrating the liquid medicine twice a day for seven days continuously. The results were: the mice do not have acute poisoning phenomenon and death within seven days.

2) Long-term toxicity test: selecting 54 healthy mice, dividing into 18 groups, taking the traditional Chinese medicine composition of examples 1-7 of the invention to obtain 1g/mL, 5g/mL and 10g/mL liquid medicines, respectively feeding the liquid medicines with different mass concentrations to each group of mice twice a day for 3 months. The results were: the compound preparation has no obvious adverse effect on various indexes of hematology, blood biochemistry, blood coagulation time, electrocardiogram and the like of mice, and no obvious special change is found in the pathological histological observation of main organs compared with a blank control group.

2. Clinical trial

120 cases of pelvic inflammation patients are selected, the patients are 22-55 years old and are averagely divided into 6 groups, the average age of each group is 35 years old, and clinical manifestations of lower abdominal pain, leucorrhea increase, menstrual disorder and soreness and pain of waist and limbs, the traditional Chinese medicine composition of the embodiment 1-6 is taken, and the traditional Chinese medicine composition is taken once every morning, noon and evening, and is continuously taken for one month, and the results are as follows:

TABLE 1 results of clinical trials

The judgment standard of the curative effect is as follows: and (3) healing: clinical symptoms disappear completely, and gynecological examination symptoms and physical signs disappear completely; the effect is shown: the clinical symptoms are obviously relieved, and the gynecological examination symptoms and signs are mostly disappeared; the method has the following advantages: the clinical symptoms are reduced, and the local symptoms and physical signs of gynecological examination are reduced; and (4) invalidation: there was no change in symptoms or signs.

As can be seen from table 1, the Chinese medicinal composition obtained in the present invention can effectively treat pelvic inflammatory disease, and example 6 has the best therapeutic effect compared to examples 1 to 5 in example 6. Specifically, in example 6, compared with examples 1 to 4, the raw materials and the operation steps are increased, in example 6, compared with example 5, the operation steps are increased, and the traditional Chinese medicine composition obtained in example 6 has a better effect of treating pelvic inflammation with the assistance of other raw materials and operation steps.

In conclusion, according to the traditional Chinese medicine composition for improving pelvic inflammation and the preparation method of the traditional Chinese medicine composition for improving pelvic inflammation provided by the embodiment of the invention, the traditional Chinese medicine composition has the effects of promoting qi and blood circulation, dispelling wind and removing dampness, and resolving masses and relieving pain, and can effectively treat qi stagnation type pelvic inflammation; the preparation method of the traditional Chinese medicine composition adopts the combination of microwave extraction and supercritical extraction, can better extract the effective components of the raw materials, and the effective components are hardly lost in the extraction process, so that the final medicine has high content of the effective components and good quality, and the medicine has better treatment effect on qi stagnation type pelvic inflammation.

The embodiments described above are some, but not all embodiments of the invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Claims

1. A traditional Chinese medicine composition for improving pelvic inflammation is characterized by comprising the following raw materials in parts by weight: 5-10 parts of herba aristolochiae, 7-20 parts of szechwan chinaberry fruit, 10-20 parts of codonopsis pilosula, 3-8 parts of Chinese violet, 4-9 parts of rhizoma arisaematis, 1-5 parts of talcum powder, 2-6 parts of radix linderae, 3-7 parts of lumbricus, 2-7 parts of myrrh, 4-10 parts of rhizoma corydalis and 1-3 parts of gentian.

2. The traditional Chinese medicine composition according to claim 1, further comprising 10-25 parts by weight of folium isatidis, 2-5 parts by weight of radix stephaniae tetrandrae, 1-5 parts by weight of semen aristolochiae, 3-7 parts by weight of sculellaria barbata, 4-8 parts by weight of rhizoma drynariae, 2-7 parts by weight of radix clematidis and 1-6 parts by weight of cornu bubali.

3. The composition of claim 2, further comprising carotene, vitamins, a-protein, amino acids, calcium stearate, and cyclodextrin.

4. A method for preparing a Chinese medicinal composition as claimed in any one of claims 1 to 3, comprising the steps of:

grinding caulis Aristolochiae, fructus Toosendan, radix Codonopsis, herba Violae, rhizoma arisaematis, radix Linderae, Lumbricus, Myrrha, rhizoma corydalis, and radix Gentianae, adding ethanol into the obtained powder, microwave extracting at 30-40 deg.C for 20-40min, filtering to obtain extractive solution and residue, and supercritical CO extracting the residue₂Fluid extraction is carried out for 15-25min to obtain extract liquor, the extract liquor and the extract liquor are mixed and then concentrated for 0.5-1h under-0.006 to-0.008 MPa, the obtained extract is dried for 10-20min at 50-60 ℃ to obtain powder, and then talcum powder and the powder are mixed.

5. The preparation method of the traditional Chinese medicine composition according to claim 4, wherein the traditional Chinese medicine composition is prepared by sieving the powder with a 70-100 mesh sieve, adding the powder into the ethanol, and soaking for 20-30 min.

6. The preparation method of the traditional Chinese medicine composition according to claim 5, wherein the mass ratio of the powder to the ethanol is 1: (1-10).

7. The method for preparing a Chinese medicinal composition according to claim 6, wherein the volume fraction of the ethanol is 70-80%.

8. The method for preparing the Chinese medicinal composition according to claim 4, wherein the microwave extraction is performed for 2 times, the first extraction is performed at 30-40 ℃ for 30-40min, and the second extraction is performed at 40-50 ℃ for 10-20 min.

9. The method as claimed in claim 8, wherein the step of mixing the extract solution and the liquid extract comprises centrifuging the mixture at 600r/min for 5-10min, and concentrating the supernatant.

10. The method for preparing a Chinese medicinal composition according to claim 9, wherein the step of mixing the talc powder with the powder further comprises stirring at 100-150r/min for 10-20min, and adding honey after mixing to prepare the water-honeyed pill.