CN113545423A - 一种基因工程实验鼠饲料及其制备方法 - Google Patents
一种基因工程实验鼠饲料及其制备方法 Download PDFInfo
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- CN113545423A CN113545423A CN202110775355.8A CN202110775355A CN113545423A CN 113545423 A CN113545423 A CN 113545423A CN 202110775355 A CN202110775355 A CN 202110775355A CN 113545423 A CN113545423 A CN 113545423A
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- vitamin
- mouse feed
- feed
- genetically engineered
- laboratory mouse
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
本发明公开了一种基因工程实验鼠饲料及其制备方法，该基因工程实验鼠饲料由如下重量份的组分组成：玉米10‑22％，小麦25‑40％，麸皮7‑20％，豆粕10‑20％，植物油1‑5％，鱼粉5‑12％，骨粉1‑5％，酪蛋白0.1‑2％，酵母粉1‑5％，复合矿物质0.1‑5％，复合维生素0.1‑1％，有机微量元素0.001‑0.005％；各组分含量之和等于100％。本发明的基因工程实验鼠饲料能够促进鼠的生长，提高鼠的繁殖率。
Description
技术领域
本发明涉及实验动物营养学技术领域，具体涉及一种基因工程实验鼠饲料及其制备方法。
背景技术
人类疾病的动物模型已经成为现代生物学和医学研究的一个必不可少的核心条件之一。由于小鼠和人类基因组的高度同源，生理生化及发育过程基本相同，对环境和药物的反应也及其相似，所以小鼠已经成为建造人类疾病相关模型及研究发病机制的最重要实验动物。除上述特点外，小鼠研究还具有成熟的基因工程改造技术手段，同时具有大量的近交系品系，实验结果有很好的一致性和可比性。小鼠作为最主要的转基因模式动物，被广泛的应用于生命科学和医药研究中。
由于基因工程实验鼠自身物种的特殊性，其具有生长缓慢、繁殖率低等特点，用常规的鼠饲料饲喂基因工程实验鼠会引起采食量下降，营养不良，无生殖能力或生殖能力差的问题，这就决定了饲料营养的特殊性。适合基因工程实验鼠的配合饲料亟待研发。
发明内容
有鉴于此，本发明的目的在于提供一种基因工程实验鼠饲料及其制备方法，该基因工程实验鼠饲料能够促进鼠的生长，提高鼠的繁殖率。
所采用的技术方案为：
本发明的一种基因工程实验鼠饲料，由如下重量份的组分组成：
各组分含量之和等于100％。
进一步地，所述植物油为花生油、豆油、蓖麻油、菜籽油、棉籽油、米糠油、玉米油中的一种或多种。
进一步地，所述复合矿物质为磷酸氢钙、碳酸钙、氯化钠、硫酸亚铁、硫酸铜、硫酸锰、硫酸镁和碘酸钙。
进一步地，所述复合维生素为维生素A、维生素D、维生素E、维生素K3、维生素B1、维生素B2、维生素B6、维生素B12、叶酸、泛酸、烟酸、生物素和氯化胆碱。
进一步地，在复合维生素中，维生素E 20-30wt％、维生素K33-5wt％、叶酸1-2wt％。
进一步地，所述有机微量元素为酵母硒和甘氨酸锌。
进一步地，所述麸皮的粒径为80-100目，所述小麦的粒径为80-100目，所述豆粕的粒径为80-100目。
本发明的一种基因工程实验鼠饲料的制备方法，包括如下步骤：
S1.原料粉碎：将玉米、小麦、豆粕分别投入到粉碎机粉碎，过80-100目筛，备用；
S2.原料混合：按照各原料的配比含量，准确称取各原料，然后将除植物油之外的其他原料投入到混合机中搅拌混合，将各原料充分混合混匀后，再加入植物油和占实验鼠饲料总重量的3％-5％的水，充分混合混匀；
S3.制粒：用小型制粒机将混合好的粉料挤压成颗粒，生产出直径10-15mm的颗粒饲料；
S4烘干：在烘干箱温度70-85℃条件下烘干。
进一步地，还包括S5.将制得的实验鼠饲料采用Co60辐照。
在上述技术方案中，在本基因工程实验鼠饲料中，
1.提高蛋白质含量，采用酪蛋白替换部分动物蛋白，增强基因工程鼠的免疫力，提高繁殖性能。
2.添加有机微量元素，如酵母硒和甘氨酸锌，吸收好，无污染，利于基因工程鼠的吸收利用，促进实验鼠生长。
3.进一步优化维生素配比，采用强化维生素比例：添加维生素E 20-30wt％、维生素K33-5wt％、叶酸1-2wt％，促进基因工程鼠的生长，提高繁殖率。
4.提高脂肪含量，适合基因工程鼠的生长和繁殖需求。
5.制备方法进一步采用Co60辐照，确保微生物指标符合SPF级实验动物标准。也能达到延长饲料保藏时间，稳定、提高饲料质量的目的。
综上，本发明的有益效果在于：
本发明的基因工程实验鼠饲料能够促进鼠的生长，提高鼠的繁殖率。
具体实施方式
下面通过具体的实施例对本发明进行详细说明，但这些例举性实施方式的用途和目的仅用来例举本发明，并非对本发明的实际保护范围构成任何形式的任何限定，更非将本发明的保护范围局限于此。
实施例1
本实施例的一种基因工程实验鼠饲料，由如下重量份的组分组成：
其中，植物油为花生油、豆油和玉米油。
复合矿物质为磷酸氢钙、碳酸钙、氯化钠、硫酸亚铁、硫酸铜、硫酸锰、硫酸镁和碘酸钙。
复合维生素为维生素A、维生素D、维生素E、维生素K3、维生素B1、维生素B2、维生素B6、维生素B12、叶酸、泛酸、烟酸、生物素和氯化胆碱。
在复合维生素中，维生素E 25wt％、维生素K34wt％、叶酸1.5wt％，复合维生素的其他组分含量为余量，总重量百分比之和为100％。
本实施例的基因工程实验鼠饲料的制备方法，包括如下步骤：
S1.原料粉碎：将玉米、小麦、豆粕分别投入到粉碎机粉碎，过80-100目筛，备用；
S2.原料混合：按照各原料的配比含量，准确称取各原料，然后将除植物油之外的其他原料投入到混合机中搅拌混合，将各原料充分混合混匀后，再加入植物油和占实验鼠饲料总重量的5％的水，充分混合混匀；
S3.制粒：用小型制粒机将混合好的粉料挤压成颗粒，生产出直径10-15mm，长度为25mm的颗粒饲料；
S4烘干：在烘干箱温度70-85℃条件下烘干。
S5.将制得的实验鼠饲料采用Co60辐照，得到Co60辐照基因工程实验鼠饲料。
实施例2
参照实施例1，与实施例1不同的是，本实施例的基因工程实验鼠饲料，由如下重量份的组分组成：
制备方法参照实施例1。
实施例3
参照实施例1，与实施例1不同的是，本实施例的基因工程实验鼠饲料，由如下重量份的组分组成：
制备方法参照实施例1。
对比例1
参照实施例2，与实施例2不同的是，本对比例的基因工程实验鼠饲料不加有机微量元素，小麦为29.5％。
对比例2
参照实施例2，与实施例2不同的是，本对比例的基因工程实验鼠饲料的复合维生素中，维生素E 8％、维生素K31.5％、叶酸0.8％。
试验测试：
试验1
将实施例2的基因工程实验鼠饲料送往谱尼测试集团上海有限公司进行测试，其检测地址在上海市徐汇区桂平路680号35幢,得到的检测报告的编号为：No.BOAJNOVT60404508,得到的检测结果参见表1。
表1
试验2
实验过程：
(1)随机选择不同亲本和不同批次3只基因工程实验雄鼠，雄鼠为8周龄、外表健康、反应灵敏、体重达标(23-25g)、毛色光亮和性器官发达，将其编号为1号、2号和3号；
(2)基因工程实验雄鼠进行单笼饲养，1号喂食实施例2的基因工程实验鼠饲料；2号喂食对比例1的基因工程实验鼠饲料；3号喂食对比例2的基因工程实验鼠饲料，自由采食和饮水，保证光照10小时、黑暗14小时间隔；
(3)随机选择不同亲本和不同批次3只基因工程实验雌鼠，雌鼠为8周龄、外表健康、反应灵敏、体重达标(20-23g)、毛色光亮和性器官发达，将其编号为4号、5号和6号；
(4)基因工程实验雌鼠进行单笼饲养，1号喂食实施例2的基因工程实验鼠饲料；2号喂食对比例1的基因工程实验鼠饲料；3号喂食对比例2的基因工程实验鼠饲料，自由采食和饮水，保证光照10小时、黑暗14小时间隔；
(5)雌鼠进行阴道涂片，观察阴道细胞变化判断情期，选择自然发情期的雌鼠用于后续自然交配；
(6)发情期雌鼠与雄鼠合笼，同时确保10-12小时的黑暗环境，使雌鼠与雄鼠1:1交配，1号雄鼠对4号雌鼠，2号雄鼠对5号雌鼠，3号雄鼠对6号雌鼠；
(7)合笼10-12小时后，检查4-5号雌鼠阴道，记录阴道状态；
(8)小心护理雌鼠和仔鼠，记录雌鼠妊娠天数、产仔数和胎儿存活数；
(9)统计四项指标，四项指标为：交配后阴道状态、妊娠天数、产仔数和胎儿存活数。
实验结果参见表2。
表2
从上表2可以看出，喂食本发明的基因工程实验鼠饲料能够提高鼠的繁殖性能，提高鼠的繁殖率。
上文所列出的一系列的详细说明仅仅是针对本发明的可行性实施例的具体说明，它们并非用以限制本发明的保护范围，凡未脱离本发明技艺精神所作的等效实施例或变更均应包含在本发明的保护范围之内。
Claims (9)
1.一种基因工程实验鼠饲料，其特征在于，由如下重量百分比的组分组成：
各组分含量之和等于100％。
2.根据权利要求1所述的基因工程实验鼠饲料，其特征在于，所述植物油为花生油、豆油、蓖麻油、菜籽油、棉籽油、米糠油、玉米油中的一种或多种。
3.根据权利要求1所述的基因工程实验鼠饲料，其特征在于，所述复合矿物质为磷酸氢钙、碳酸钙、氯化钠、硫酸亚铁、硫酸铜、硫酸锰、硫酸镁和碘酸钙。
4.根据权利要求1所述的基因工程实验鼠饲料，其特征在于，所述复合维生素为维生素A、维生素D、维生素E、维生素K3、维生素B1、维生素B2、维生素B6、维生素B12、叶酸、泛酸、烟酸、生物素和氯化胆碱。
5.根据权利要求4所述的基因工程实验鼠饲料，其特征在于,在复合维生素中，维生素E20-30wt％、维生素K3 3-5wt％、叶酸1-2wt％。
6.根据权利要求1所述的基因工程实验鼠饲料，其特征在于，所述有机微量元素为酵母硒和甘氨酸锌。
7.根据权利要求1所述的基因工程实验鼠饲料，其特征在于，所述麸皮的粒径为80-100目，所述小麦的粒径为80-100目，所述豆粕的粒径为80-100目。
8.一种权利要求1-7任一所述的基因工程实验鼠饲料的制备方法，其特征在于，包括如下步骤：
S1.原料粉碎：将玉米、小麦、豆粕分别投入到粉碎机粉碎，过80-100目筛，备用；
S2.原料混合：按照各原料的配比含量，准确称取各原料，然后将除植物油之外的其他原料投入到混合机中搅拌混合，将各原料充分混合混匀后，再加入植物油和占实验鼠饲料总重量的3％-5％的水，充分混合混匀；
S3.制粒：用小型制粒机将混合好的粉料挤压成颗粒，生产出直径10-15mm的颗粒饲料；
S4烘干：在烘干箱温度70-85℃条件下烘干。
9.根据权利要求8所述的基因工程实验鼠饲料的制备方法，其特征在于，还包括S5.将制得的实验鼠饲料采用Co60辐照。
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| CN109123079A (zh) * | 2018-09-27 | 2019-01-04 | 江苏省协同医药生物工程有限责任公司 | 一种转基因实验鼠饲料及其制备方法 |
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| CN107048048A (zh) * | 2017-05-27 | 2017-08-18 | 武汉市万千佳兴生物科技有限公司 | 一种基因编辑实验鼠饲料 |
| CN109123079A (zh) * | 2018-09-27 | 2019-01-04 | 江苏省协同医药生物工程有限责任公司 | 一种转基因实验鼠饲料及其制备方法 |
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