CN113533758A - Biochemical photoelectric detection system and biochemical analyzer - Google Patents
Biochemical photoelectric detection system and biochemical analyzer Download PDFInfo
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- CN113533758A CN113533758A CN202110725087.9A CN202110725087A CN113533758A CN 113533758 A CN113533758 A CN 113533758A CN 202110725087 A CN202110725087 A CN 202110725087A CN 113533758 A CN113533758 A CN 113533758A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
Abstract
The application relates to a biochemical photoelectric detection system and biochemical analyzer, including: the LED light source group comprises a preset number of LED light sources, an optical filter, a light splitting element and a plurality of photoelectric detectors; each LED light source is correspondingly provided with an optical filter, each optical filter transmits monochromatic light beams with preset wavelengths required by biochemical photoelectric detection, and the corresponding preset wavelengths of the monochromatic light beams transmitted through the optical filters are different; each LED light source is lightened in a time-sharing lightening mode; emitting an incident light beam with at least one preset wavelength required by biochemical photoelectric detection when one LED light source is lightened each time; the optical filter performs monochromatic processing on incident beams emitted by the corresponding LED light sources to obtain monochromatic beams with corresponding preset wavelengths; the light splitting element splits the monochromatic light beam into a plurality of monochromatic light beams which are respectively incident to the detection channels; each photodetector receives the optical signal of the monochromatic light beam after passing through the corresponding detection channel. The system has the advantages of high detection speed, high detection precision, small size and low cost.
Description
Technical Field
The application relates to the technical field of biochemical photoelectric detection, in particular to a biochemical photoelectric detection system and a biochemical analyzer.
Background
A biochemical analyzer based on spectrophotometry is an analyzer for obtaining various biochemical indexes (such as blood routine, liver function, kidney function, heart function, blood sugar, blood fat, mineral substances and the like) in human body fluid (blood and urine) by utilizing Lambert beer's law, can accurately and quickly provide required test data for doctors and chemical testers, and has important roles in clinical diagnosis and chemical test. The typical components of the biochemical analyzer comprise an optical detection system, a control system and a data processing system; the optical detection system is the premise of rapid and accurate detection of an automatic biochemical analyzer, and in addition, the optical detection system determines the development trend of the analyzer, namely miniaturization, automation, precision and multi-parameterization. The biochemical analyzer has two photoelectric colorimetric methods, namely a front light splitting technology and a rear light splitting technology, wherein the front light splitting technology can split a polychromatic light beam emitted by a light source through a monochromator and then enter a detection channel, and then biochemical photoelectric detection under corresponding preset wavelength is carried out on the basis of the monochromatic light beam passing through the detection channel. In addition, the detection items of the biochemical analyzer are usually used at the wavelength of 300-800nm, and a halogen lamp is usually used as a light source, but the halogen lamp has the defects of short service life, need of replacing the light source regularly, high heat generation and need of designing a special heat dissipation device, so that the development trend of the biochemical analyzer towards miniaturization and low maintenance cost is limited; the monochromator is a component which enables light with different wavelengths to be diffused at different angles, the monochromator commonly used by the biochemical analyzer is a grating, and the grating as the monochromator has the main defects of high price, secondary spectrum interference analysis and large stray light influence, so that the development of the biochemical analyzer towards low cost and high detection precision is limited.
Disclosure of Invention
In view of the above, there is a need to provide a biochemical photoelectric detection system and a biochemical analyzer that can achieve high detection speed, high detection accuracy, low cost and small size.
A biochemical photodetection system comprising: the LED light source group, the optical filter, the light splitting element and the plurality of photoelectric detectors; the LED light source group comprises a preset number of LED light sources; each LED light source is correspondingly provided with an optical filter, each optical filter is used for transmitting monochromatic light beams with preset wavelength required by biochemical photoelectric detection, and the corresponding preset wavelengths of the monochromatic light beams transmitted through the optical filters are different; the preset number of LED light sources are lightened in a time-sharing lightening mode;
emitting an incident light beam with at least one preset wavelength required by biochemical photoelectric detection when one LED light source is lightened each time;
the optical filter is used for carrying out monochromatic processing on incident beams emitted by the corresponding LED light sources to obtain monochromatic beams with corresponding preset wavelengths;
the light splitting element is used for splitting the monochromatic light beams into a plurality of monochromatic light beams which are respectively incident to a detection channel;
each photodetector is configured to receive an optical signal of the monochromatic light beam after passing through a corresponding detection channel.
In one embodiment, the number of the LED light source groups is at least two; the system further comprises: a light converging element;
the light collecting element is used for collecting the monochromatic light beams transmitted through the optical filters to the light splitting element.
In one embodiment, a light homogenizing rod is arranged between the light collecting element and the light splitting element.
In one embodiment, the system further comprises a condenser lens group; the arrangement position of the condenser lens group includes at least one of:
the optical filter is arranged between the light converging element and the light source;
the light converging element is arranged between the light homogenizing rod and the light source;
the light splitting element and the detection channel;
the detection channel and the photoelectric detector.
In one embodiment, the predetermined wavelength required for the biochemical photoelectric detection is one or more of 340nm, 380nm, 405nm, 450nm, 480nm, 500nm, 545nm, 570nm, 600nm, 660nm, 700nm, 750nm and 800 nm.
In one embodiment, each of the detection channels shares the same cuvette, the cuvette comprises a plurality of cuvettes, and rotation of the cuvette causes each cuvette to be transmitted sequentially by a beam of monochromatic light incident on each of the detection channels.
In one embodiment, the LED light sources in each LED light source group are arranged in a circle
In one embodiment, the preset number of LED light sources are cyclically lighted by a time-sharing lighting manner.
In one embodiment, the light splitting element is a fiber optic bundle, a beam splitter, or a dichroic mirror.
According to the biochemical photoelectric detection system, the LED light source group comprising the LED light sources with the preset number is used as the system light source, and the LED light sources have the advantages that the service life is long, the light sources do not need to be replaced regularly, the heating is low, and a special heat dissipation device does not need to be designed, so that the system can be developed towards the direction of small size and low cost. In addition, a preset number of LED light sources are lighted in a time-sharing lighting mode, light filters capable of transmitting monochromatic light beams with a preset wavelength required by biochemical photoelectric detection are respectively arranged behind each LED light source, the preset wavelengths transmitted by the light filters are different, so that the monochromatic light beams with at most one preset wavelength at the same time are divided into a plurality of monochromatic light beams by the light splitting element and then enter the detection channels, the biochemical photoelectric detection with the same preset wavelength can be simultaneously carried out on the detection channels, and the biochemical photoelectric detection under each preset wavelength can be realized in the process of sequentially lighting the LED light sources according to the time-sharing lighting mode, so that the detection speed can be improved. In addition, because the biochemical photoelectric detection system adopts the optical filter which is arranged corresponding to each LED light source and can transmit one preset wavelength required by biochemical photoelectric detection as the monochromator, and does not adopt the grating as the monochromator, the problem that the system is limited to be developed towards low cost and high detection precision because the grating is adopted as the monochromator can be avoided. In addition, the monochromatic light beams transmitted through the optical filter are split into a plurality of monochromatic light beams by the light splitting element, and the plurality of monochromatic light beams are respectively incident to one detection channel so as to realize a plurality of biochemical photoelectric detections under the same preset wavelength, so that the detection speed can be further improved. In summary, the biochemical photoelectric detection system has the advantages of high detection speed, high detection precision, small size and low cost.
A biochemical analyzer comprises the biochemical photoelectric detection system provided in the system embodiments.
The biochemical photoelectric detection system provided by each system embodiment has the advantages of high detection speed, high detection precision, small size and low cost, so that the biochemical analyzer adopting the biochemical photoelectric detection system also has the advantages of high detection speed, high detection precision, small size and low cost.
Drawings
FIG. 1 is a schematic diagram of the structure of a biochemical photoelectric detection system in one embodiment;
FIG. 2 is a schematic cross-sectional view of a plurality of LED light sources and corresponding filters arranged circumferentially in accordance with one embodiment;
FIG. 3 is a schematic diagram of an optical path structure of a biochemical photoelectric detection system in one embodiment;
FIG. 4 is a schematic diagram of an optical path structure of a biochemical photoelectric detection system in another embodiment.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more apparent, the present application is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.
In one embodiment, as shown in FIG. 1, there is provided a biochemical photodetection system comprising: the LED light source group 100, the optical filter 200, the light splitting element 300 and the photoelectric detector 400; the LED light source group 100 includes a preset number of LED light sources; each LED light source is correspondingly provided with one optical filter 200, each optical filter 200 is used for transmitting monochromatic light beams with a preset wavelength required by biochemical photoelectric detection, and the corresponding preset wavelengths of the monochromatic light beams transmitted through the optical filters 200 are different; the LED light sources with the preset number are lightened in a time-sharing lightening mode; emitting an incident light beam with at least one preset wavelength required by biochemical photoelectric detection when one LED light source is lightened each time; the optical filter 200 is used for performing monochromatic processing on incident beams emitted by corresponding LED light sources to obtain monochromatic beams with corresponding preset wavelengths; the light splitting element 300 is used for splitting the monochromatic light beam into a plurality of monochromatic light beams which are respectively incident to a detection channel; there are a plurality of photodetectors 400, each photodetector 400 being configured to receive an optical signal of a monochromatic light beam after passing through a corresponding detection channel.
If the biochemical photoelectric detection system is required to realize biochemical photoelectric detection under a preset number of preset wavelengths, a preset number of LED light sources are required to be arranged in an LED light source group of the biochemical photoelectric detection system, and the preset number can be customized according to actual requirements of biochemical photoelectric detection, for example, 13.
In one embodiment, the LED light sources in each LED light source group are arranged in a circle. The circumferential arrangement may mean that the LED light sources in each LED light source group are uniformly distributed on the circumference, or that one of the LED light sources is located at the center of a circle, and the rest of the LED light sources are uniformly distributed on the circumference around the center of the circle. It is understood that the arrangement of the LED light sources in each LED light source group is not limited to the circumferential arrangement, and may also be a rectangular arrangement, which is not limited in this respect. In this embodiment, the plurality of LED light sources in each LED light source group are arranged in a circumferential manner, so that the arrangement of the plurality of LED light sources is more compact, and the size of the LED light source group can be reduced.
In one embodiment, if the LED light sources in the same LED light source group are arranged circumferentially, the light filters corresponding to the LED light sources in the LED light source group are also arranged circumferentially.
As shown in fig. 2, in an embodiment, a schematic cross-sectional view of a plurality of LED light sources and corresponding filters arranged in a circle is provided, and taking an example that the LED light source group 100 includes 7 LED light sources, the 7 LED light sources are arranged in a circle, wherein 1 LED light source is located at a center of the circle, the remaining 6 LED light sources are uniformly arranged on the circle around the center of the circle, and the filters 200 corresponding to the 7 LED light sources are arranged in the same circle arrangement manner. It is understood that the number of LED light sources and the circumferential disposition of the LED light sources shown in fig. 2 are only examples, and are not limited to specific limitations, for example, the 7 LED light sources are all uniformly distributed on the circumference.
The time-sharing lighting mode is that each LED light source is sequentially lighted, and at most one LED light source is in a lighting state at the same time point, so that the time-sharing lighting of the LED light sources with a preset number can be realized by setting the lighting sequence of each LED light source and the single lighting time length of each LED light source, and the single lighting time length can be defined according to actual requirements, such as 1ms (millisecond, a time unit). Each LED light source, when illuminated, emits an incident light beam covering at least one predetermined wavelength required for biochemical photodetection. The LED light source may be a broad spectrum LED lamp, such as a broad spectrum LED lamp with a wavelength range of 400nm-1000nm (nanometers, a length measurement unit), a single color LED lamp, such as a single color LED lamp with a wavelength of 340nm or 380nm, a multi-color LED lamp, such as a multi-color LED lamp with a wavelength range of 720nm-820nm, or a white light LED lamp, such as a white light LED lamp with a wavelength range of 400nm-740nm, which is not limited herein.
The light filters arranged correspondingly to each LED light source can transmit monochromatic light beams with preset wavelengths required by biochemical photoelectric detection, and each light filter respectively transmits the monochromatic light beams with different preset wavelengths. Incident beams emitted by each LED light source are incident to the corresponding optical filter, and only the monochromatic beams with the corresponding preset wavelength in the incident beams can transmit through the optical filter, so that the monochromatic beams with the corresponding preset wavelength can be obtained. The light splitting element splits the monochromatic light beams with the preset wavelength transmitted by the light filters into a plurality of monochromatic light beams with the preset wavelength, and each monochromatic light beam is respectively incident to one detection channel, so that each detection channel at the same time point at most passes through one monochromatic light beam with the preset wavelength, and the wavelength of the monochromatic light beam passing through each detection channel at the same time point is the same preset wavelength.
Be provided with detection device in the detection channel, detection device specifically can be the cell or includes the color comparison disc of a plurality of cells, be equipped with the material to be measured that awaits measuring of biochemical photoelectric detection in the cell, like this, when the monochromatic beam of incidence passes through the detection channel, can transmit the material to be measured that sets up in this single cell in detecting the channel, from this, the monochromatic beam after through detecting the channel is the monochromatic beam who transmits the material to be measured promptly, based on this monochromatic beam who transmits the material to be measured, can realize the biochemical photoelectric detection of the material to be measured under corresponding wavelength of predetermineeing. The number of the photoelectric detectors is consistent with that of the detection channels, when a plurality of monochromatic light beams with the same preset wavelength are respectively incident to the corresponding detection channels, each photoelectric detector is used for receiving optical signals of the monochromatic light beams passing through the corresponding detection channels, and therefore a plurality of biochemical photoelectric detections under the single preset wavelength can be simultaneously realized based on the monochromatic light beams passing through the detection channels, and the detection speed can be improved.
In one embodiment, the predetermined wavelength required for biochemical photodetection includes, but is not limited to, one or more of 340nm, 380nm, 405nm, 450nm, 480nm, 500nm, 545nm, 570nm, 600nm, 660nm, 700nm, 750nm, and 800 nm. Taking the example that the biochemical photoelectric detection system can realize the biochemical photoelectric detection under the 13 preset wavelengths, the LED light source group in the biochemical photoelectric detection system includes 13 LED light sources, and the 13 LED light sources may be monochromatic LED lamps with wavelengths respectively corresponding to the preset wavelengths, and are respectively used for emitting incident light beams with corresponding preset wavelengths, or 11 broad-spectrum LED lamps with a wavelength range of 400nm to 1000nm, a monochromatic LED lamp with a wavelength of 340nm, and a monochromatic LED lamp with a wavelength of 380 nm. It is understood that the combination manner of the LED light sources is not limited to the above example, as long as the biochemical photoelectric detection at the 13 preset wavelengths can be respectively realized based on the respective LED light sources which are sequentially turned on.
According to the biochemical photoelectric detection system, the LED light source group comprising the LED light sources with the preset number is used as the system light source, and the LED light sources have the advantages that the service life is long, the light sources do not need to be replaced regularly, the heating is low, and a special heat dissipation device does not need to be designed, so that the system can be developed towards the direction of small size and low cost. In addition, a preset number of LED light sources are lighted in a time-sharing lighting mode, light filters capable of transmitting monochromatic light beams with a preset wavelength required by biochemical photoelectric detection are respectively arranged behind each LED light source, the preset wavelengths transmitted by the light filters are different, so that the monochromatic light beams with at most one preset wavelength at the same time are divided into a plurality of monochromatic light beams by the light splitting element and then enter the detection channels, the biochemical photoelectric detection with the same preset wavelength can be simultaneously carried out on the detection channels, and the biochemical photoelectric detection under each preset wavelength can be realized in the process of sequentially lighting the LED light sources according to the time-sharing lighting mode, so that the detection speed can be improved. In addition, because the biochemical photoelectric detection system adopts the optical filter which is arranged corresponding to each LED light source and can transmit one preset wavelength required by biochemical photoelectric detection as the monochromator, and does not adopt the grating as the monochromator, the problem that the system is limited to be developed towards low cost and high detection precision because the grating is adopted as the monochromator can be avoided. In addition, the monochromatic light beams transmitted through the optical filter are split into a plurality of monochromatic light beams by the light splitting element, and the plurality of monochromatic light beams are respectively incident to one detection channel so as to realize a plurality of biochemical photoelectric detections under the same preset wavelength, so that the detection speed can be further improved. In summary, the biochemical photoelectric detection system has the advantages of high detection speed, high detection precision, small size and low cost.
In one embodiment, the number of LED light source groups is at least two; the biochemical photoelectric detection system further comprises: a light converging element; the light collecting element is used for collecting the monochromatic light beams transmitted by the optical filters to the light splitting element.
Each LED light source group comprises at least one LED light source, and the total number of the LED light sources in each LED light source group is a preset number. Because the preset number of LED light sources are lightened in a time-sharing lightening mode, when one LED light source is lightened each time, the light converging element only receives one monochromatic light beam, the monochromatic light beam is obtained based on the incident light beam emitted by the LED light source which is lightened currently, and the light converging element converges the received monochromatic light beam to the light splitting element. In the present embodiment, the light collecting element is used to make monochromatic light beams from different LED light sources reach the same light path. When different LED light sources are lightened in sequence, the monochromatic light beams corresponding to the LED light sources can be sequentially converged to the light splitting element through the light converging element, so that the monochromatic light beams from the different LED light sources are enabled to be continuously transmitted through the same light path through the light converging operation of the light converging element, the biochemical photoelectric detection under the preset wavelength of the preset number is guaranteed, the light path structure of the biochemical photoelectric detection system can be simplified, and the size of the system is reduced.
In one embodiment, the light collection element is a dichroic mirror, a beam splitter, or a fiber optic bundle. When the light converging element is a dichroic mirror or a spectroscope, the number of the light converging elements is one less than that of the LED light source groups, for example, if the number of the LED light source groups is two, the number of the light converging elements is one, if the number of the LED light source groups is three, the number of the light converging elements is two, and so on, so as to converge the monochromatic light beams corresponding to the LED light source groups to the light splitting element. When the light converging element is an optical fiber bundle, the optical fiber bundle is a multi-transmission optical fiber bundle, the number of the optical fiber bundles is irrelevant to the number of the LED light source groups, for example, a single optical fiber bundle can converge monochromatic light beams corresponding to a plurality of LED light source groups to the light splitting element.
As shown in fig. 3, in one embodiment, a schematic diagram of an optical path structure of a biochemical photoelectric detection system is provided, the biochemical photoelectric detection system includes: the LED light source assembly 100, the optical filter 200, the light splitting element 300, the photodetector 400 and the light collecting element 500; wherein, there are two LED light source groups 100, which are respectively represented by reference numerals 101 and 102, two detection channels, which are respectively represented by reference numerals 001 and 002, two photodetectors 400, which are respectively represented by reference numerals 401 and 402, an optical filter 200 is arranged between each LED light source in each LED light source group 100 and the light converging element 500, each optical filter is used for transmitting a monochromatic light beam with a preset wavelength required by biochemical photoelectric detection, the corresponding preset wavelengths of the monochromatic light beams transmitted through each optical filter are different, the monochromatic light beams passing through the light collecting element 500 are incident to the light splitting element for light splitting to obtain two monochromatic light beams, one beam is incident to the detection channel 001, the other beam is incident to the detection channel 002, the photoelectric detector 401 receives optical signals of the monochromatic light beams passing through the detection channel 001, and the photoelectric detector 402 receives optical signals of the monochromatic light beams passing through the detection channel 002.
It is understood that the number of LED light source sets, the number of light collecting elements, the type of light splitting element, the number of detection channels and photodetectors, and the like in fig. 3 are only used as examples and are not limited to specific limitations. It should be noted that, in fig. 3, only one filter is illustrated between each LED light source group and the light collecting element, and the number of the filters is substantially the same as the number of the LED light sources in the corresponding LED light source group.
In one embodiment, a light homogenizing rod is arranged between the light collecting element and the light splitting element. The light homogenizing rod may be a hexagonal light homogenizing rod, such as a quartz hexagonal light homogenizing rod, for homogenizing the light spot of the incident monochromatic light beam. The monochromatic light beams collected by the light collecting element penetrate through the light homogenizing rod and then enter the light splitting element, so that the light spots of the monochromatic light beams entering the light splitting element are more uniform, the light energy among the monochromatic light beams obtained by splitting the monochromatic light beams by the light splitting element is more uniform, and the light energy deviation of the monochromatic light beams entering the detection channels can be reduced.
In one embodiment, when the biochemical photodetection system comprises a single LED light source group, an integrator rod may be disposed between the single LED light source group and the detection channel.
In one embodiment, the light splitting element is a fiber optic bundle, a beam splitter, or a dichroic mirror. When the optical fiber bundle is used as a light splitting element, the optical fiber bundle is a light-splitting optical fiber bundle. It is understood that when the light splitting element is a fiber bundle, the number of the fiber bundles is independent of the number of the detection channels, for example, one fiber bundle can split a monochromatic light beam into monochromatic light beams with the number consistent with the number of the detection channels. When the light splitting element is a light splitting sheet or a dichroic mirror, the number of the light splitting element is one less than the number of the detection channels, for example, if the number of the detection channels is two, the number of the light splitting element is one, if the number of the detection channels is three, the number of the light splitting element is two, and so on, so as to split a monochromatic light beam into monochromatic light beams the number of which is the same as the number of the detection channels.
In one embodiment, the biochemical photoelectric detection system further comprises a condenser lens group; the arrangement position of the condenser lens group includes at least one of: between the optical filter and the light converging element; between the light converging element and the light homogenizing rod; between the light splitting element and the detection channel; between the detection channel and the photodetector.
The condensing lens group includes one or more condensing lenses, and the condensing lens may specifically be a spherical lens and/or an aspheric lens, and when the condensing lens group includes a plurality of condensing lenses, the condensing lens group may specifically be a plurality of spherical lenses or a plurality of aspheric lenses, or a combination of a spherical lens and an aspheric lens. The light beam incident to the condenser lens group is condensed or focused through the condenser lens group, so that the energy of the light beam is more uniform, and the detection precision can be improved.
As shown in fig. 4, in one embodiment, a schematic diagram of an optical path structure of a biochemical photoelectric detection system is provided, the biochemical photoelectric detection system includes: the LED light source group 100, the optical filter 200, the light splitting element 300, the photoelectric detector 400, the light converging element 500, the light homogenizing rod 600 and the condensing lens group 700; the three LED light source groups 100 are respectively represented by reference numerals 101, 102 and 103, the two light converging elements 500 are respectively represented by reference numerals 501 and 502, the two detection channels are respectively represented by reference numerals 001 and 002, the two photodetectors 400 are respectively represented by reference numerals 401 and 402, and the light converging lens group 700 is respectively disposed between the light filter 200 behind the LED light source group 101 and the light converging element 501, between the light filter 200 behind the LED light source group 102 and the light converging element 501, between the light filter 200 behind the LED light source group 103 and the light converging element 502, between the light converging element 502 and the light homogenizing rod 600, between the light splitting element 300 and the detection channel 001, between the light splitting element 300 and the detection channel 002, between the detection channel 001 and the photodetector 401, and between the detection channel 002 and the photodetector 402.
It can be understood that, taking the light beam propagation direction as a reference, the filter 200 is disposed behind each LED light source group, the light converging element 502 is disposed behind the light converging element 501, and the light splitting element 300 is disposed behind the light homogenizing rod 600. The number of LED light source groups, the number of light converging elements, the type of light splitting element, the number of detection channels and photodetectors, the number and arrangement positions of the condensing lens groups, and the like in fig. 4 are only examples and are not limited to specific ones. For example, one or more of the condenser lens groups shown in fig. 4 may be reduced as the case may be. It should be noted that, in fig. 4, only one filter is illustrated between each LED light source group and the light collecting element, and the number of the filters is substantially the same as the number of the LED light sources in the corresponding LED light source group.
In one embodiment, each detection channel shares the same color wheel; the cuvette comprises a plurality of cuvettes; and the colorimetric discs are rotated to ensure that the colorimetric cups are sequentially transmitted by the monochromatic light beams incident to the detection channels.
Wherein, the detection device who sets up in the measuring channel specifically can be the color disc, is provided with a plurality of cuvettes on the color disc, and each measuring channel in above-mentioned biochemical photoelectric detection system shares a color disc, and the monochromatic light beam of inciding to different measuring channels passes different cuvettes in the color disc to realize a plurality of biochemical photoelectric detection under the same wavelength of predetermineeing. Further, the cuvette provided with a plurality of cuvettes is rotatable, and each cuvette is transmitted by the monochromatic light beams incident to each detection channel in sequence by rotating the cuvette, so that biochemical photoelectric detection of the substance to be detected in the cuvette can be realized based on the monochromatic light beams transmitted through the cuvette. Like this, a plurality of measuring channel share the same, and rotatable color comparison dish, through the rotational speed that sets up the color comparison dish, can realize high speed and biochemical photoelectric detection of high accuracy for the photoelectric detection system can develop to the direction of small-size.
In one embodiment, the preset number of LED light sources are cyclically lit by a time-sharing lighting manner.
The step of cyclically lighting the preset number of LED light sources in a time-sharing lighting mode refers to cyclically lighting the LED light sources in sequence according to a preset period. The preset period is determined by the preset number of the LED light sources and the single lighting time of each LED light source, for example, the preset number is 13, the single lighting time of each LED light source is 1ms, and the preset period is 13 ms. By adopting the lighting mode, the generation speed of the monochromatic light beams corresponding to the preset wavelengths can be ensured, and high-speed full-automatic biochemical photoelectric detection can be realized.
In one embodiment, the timing control circuit controls the LED light sources to circularly light according to a time-sharing lighting mode. Therefore, each cuvette in the cuvette can be ensured to be transmitted by the monochromatic light beams with the preset wavelengths through time sequence control, so that biochemical photoelectric detection under the preset wavelengths can be realized for the substances to be detected in each cuvette. And the sequential control circuit controls the lighting of each LED light source so as to realize the switching of the preset wavelength, and the switching of the preset wavelength is not required to be realized through mechanical movement, so that the detection precision can be ensured, and the detection speed can be improved.
It should be understood that, in the schematic diagrams of the optical path structures shown in fig. 3 and 4, the schematic structures and shapes of the optical elements are only used as examples and are not intended to be limited specifically, and the relative distances between the optical elements in the schematic diagrams of the optical path structures are only used as examples and are not intended to be limited specifically to the actual relative distances in the LED light source-based biochemical photoelectric detection system.
In one embodiment, a biochemical analyzer includes the biochemical photodetection system provided in the system embodiments described above.
The biochemical photoelectric detection system provided in one or more embodiments above may be applied to a biochemical analyzer, so that the biochemical analyzer realizes biochemical photoelectric detection corresponding to each preset wavelength based on the biochemical photoelectric detection system. The biochemical photoelectric detection system provided in the above embodiment has the advantages of high detection speed, high detection precision, small size and low cost, and thus, the biochemical analyzer using the biochemical photoelectric detection system also has the advantages of high detection speed, high detection precision, small size and low cost.
The technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present application, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the concept of the present application, which falls within the scope of protection of the present application. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. A biochemical photodetecting system, characterized in that it comprises: the LED light source group, the optical filter, the light splitting element and the plurality of photoelectric detectors; the LED light source group comprises a preset number of LED light sources; each LED light source is correspondingly provided with an optical filter, each optical filter is used for transmitting monochromatic light beams with preset wavelength required by biochemical photoelectric detection, and the corresponding preset wavelengths of the monochromatic light beams transmitted through the optical filters are different; the preset number of LED light sources are lightened in a time-sharing lightening mode;
emitting an incident light beam with at least one preset wavelength required by biochemical photoelectric detection when one LED light source is lightened each time;
the optical filter is used for carrying out monochromatic processing on incident beams emitted by the corresponding LED light sources to obtain monochromatic beams with corresponding preset wavelengths;
the light splitting element is used for splitting the monochromatic light beams into a plurality of monochromatic light beams which are respectively incident to a detection channel;
each photodetector is configured to receive an optical signal of the monochromatic light beam after passing through a corresponding detection channel.
2. The system of claim 1, wherein the LED light source groups are at least two; the system further comprises: a light converging element;
the light collecting element is used for collecting the monochromatic light beams transmitted through the optical filters to the light splitting element.
3. The system of claim 2, wherein a light homogenizing rod is disposed between the light collecting element and the light splitting element.
4. The system of claim 3, further comprising a condenser lens group; the arrangement position of the condenser lens group includes at least one of:
the optical filter is arranged between the light converging element and the light source;
the light converging element is arranged between the light homogenizing rod and the light source;
the light splitting element and the detection channel;
the detection channel and the photoelectric detector.
5. The system of claim 1, wherein the predetermined wavelength required for the biochemical photodetection is one or more of 340nm, 380nm, 405nm, 450nm, 480nm, 500nm, 545nm, 570nm, 600nm, 660nm, 700nm, 750nm, and 800 nm.
6. The system of claim 1 wherein each of the detection channels share the same cuvette, the cuvette comprising a plurality of cuvettes, rotation of the cuvette causing each cuvette to be sequentially transmitted by a beam of monochromatic light incident on each of the detection channels.
7. The system of claim 1, wherein the LED light sources within each LED light source group are arranged circumferentially.
8. The system of any one of claims 1 to 7, wherein the predetermined number of LED light sources are cyclically illuminated in a time-shared manner.
9. The system of claim 8, wherein the light-splitting element is a fiber optic bundle, a beam splitter, or a dichroic mirror.
10. A biochemical analyzer, comprising the biochemical photodetection system according to any one of claims 1 to 9.
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