CN219871188U - Liquid chromatography detector - Google Patents
Liquid chromatography detector Download PDFInfo
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- CN219871188U CN219871188U CN202321116575.0U CN202321116575U CN219871188U CN 219871188 U CN219871188 U CN 219871188U CN 202321116575 U CN202321116575 U CN 202321116575U CN 219871188 U CN219871188 U CN 219871188U
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- light
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- light beam
- wavelength
- converging lens
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- 238000004811 liquid chromatography Methods 0.000 title claims abstract description 32
- 238000001514 detection method Methods 0.000 claims abstract description 82
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 230000003287 optical effect Effects 0.000 claims description 12
- 238000000576 coating method Methods 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 239000011248 coating agent Substances 0.000 claims 1
- 238000002835 absorbance Methods 0.000 abstract description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- YZCKVEUIGOORGS-OUBTZVSYSA-N Deuterium Chemical compound [2H] YZCKVEUIGOORGS-OUBTZVSYSA-N 0.000 description 1
- 102000017011 Glycated Hemoglobin A Human genes 0.000 description 1
- 108010014663 Glycated Hemoglobin A Proteins 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 229910052805 deuterium Inorganic materials 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The present utility model relates to a liquid chromatography detector. The liquid chromatography detector includes: the detection light module is used for emitting a first wavelength light beam to the first surface of the light converging lens; a reference light module for emitting a second wavelength light beam to a second surface of the light converging lens; the first wavelength light beam and the second wavelength light beam are coaxially transmitted and irradiated to a sample flow cell arranged behind the light converging lens after being converged by the light converging lens; a converging lens, a light-passing hole and a spectroscope are sequentially arranged behind the sample flow cell; a detection light detection module for detecting the detection light beam of the first wavelength split by the spectroscope; a reference light detection module for detecting a reference light beam of a second wavelength split by the beam splitter; and the main control module is respectively connected with the detection light detection module and the reference light detection module and is used for determining a liquid chromatography detection result according to the reference light beam signal and the detection light beam signal. The detector can detect the corresponding liquid chromatographic absorbance.
Description
Technical Field
The utility model relates to the field of liquid chromatography detection, in particular to a liquid chromatography detector.
Background
The detection principle of glycosylated hemoglobin detection is liquid chromatography, which irradiates a liquid sample to be detected flowing through a sample flow cell through a light source, and then obtains the absorbance value of the sample to be detected, thereby completing the detection of the sample to be detected.
In liquid chromatography detection, the use of ultraviolet detectors is very widespread. Deuterium or halogen lamps are commonly used in the prior art as variable wavelength detectors, and in some specific usage scenarios, two fixed wavelengths are used as detection light sources. The LED with fixed wavelength is used as a detection light source, the service life of the lamp is longer, and the manufacturing cost is lower. There are increasing applications in the fields related to liquid chromatography.
Disclosure of Invention
The utility model provides a liquid chromatography detector, which aims to perform liquid chromatography detection by using two light sources with two fixed wavelengths through double channels.
An embodiment of the present utility model provides a liquid chromatography detector including:
the detection light module is used for emitting a first wavelength light beam to the first surface of the light converging lens;
a reference light module for emitting a second wavelength light beam to a second surface of the light converging lens;
the first wavelength light beam and the second wavelength light beam are coaxially transmitted and irradiated to a sample flow cell arranged behind the light converging lens after being converged by the light converging lens;
a converging lens, a light-passing hole and a spectroscope are sequentially arranged behind the sample flow cell;
a detection light detection module for detecting the detection light beam of the first wavelength split by the spectroscope;
a reference light detection module for detecting a reference light beam of a second wavelength split by the beam splitter;
and the main control module is respectively connected with the detection light detection module and the reference light detection module and is used for determining a liquid chromatography detection result according to the reference light beam signal and the detection light beam signal.
According to the liquid chromatography detector provided by the embodiment of the utility model, the first wavelength light beam and the second wavelength light beam are combined to irradiate the sample to be detected, and then the reference light beam and the detection light beam are obtained through light splitting, so that the absorbance value of the sample to be detected is determined. The converging lens is arranged behind the sample flow cell to focus the light beam, and the light-transmitting hole is utilized to reduce the reflection influence of the light, so that the liquid chromatography detection of the double-channel fixed wavelength is realized, and the light transmission path of the detector is simpler.
Drawings
Fig. 1 is a schematic structural diagram of a liquid chromatography detector according to an embodiment of the present utility model;
fig. 2 is a schematic diagram of a main structure of a liquid chromatography detector according to an embodiment of the utility model.
Detailed Description
The utility model is described in further detail below with reference to the drawings and examples. It is to be understood that the specific embodiments described herein are merely illustrative of the utility model and are not limiting thereof. It should be further noted that, for convenience of description, only some, but not all of the structures related to the present utility model are shown in the drawings.
Examples
Fig. 1 is a schematic structural diagram of a liquid chromatography detector according to an embodiment of the present utility model, as shown in fig. 1, the liquid chromatography detector includes:
a detection light module 1 for emitting a first wavelength light beam to a first surface of the light converging lens 2;
a reference light module 2 for emitting a second wavelength light beam to a second surface of the light combining mirror 3;
the first wavelength light beam and the second wavelength light beam are coaxially transmitted and irradiated to a sample flow cell 4 arranged behind the light combining lens 3 after being combined by the light combining lens 3;
a converging lens 5, a light-passing hole 6 and a spectroscope 7 are sequentially arranged behind the sample flow cell 4;
a detection light detection module 8 for detecting the detection light beam of the first wavelength split by the beam splitter 7;
a reference light detection module 9 for detecting the reference light beam of the second wavelength split by the beam splitter 7;
and the main control module is respectively connected with the detection light detection module 8 and the reference light detection module 9 and is used for determining a liquid chromatography detection result according to the reference light beam signal and the detection light beam signal.
Wherein, the first wavelength light beam of the detection optical module 1 and the second wavelength light beam of the reference optical module 2 have different wavelengths. In this embodiment, two different LED lamps with a single wavelength may be used as the light source, one being a test wave light source and one being a reference wave light source. The selection of the wavelengths of the two light sources is related to the liquid phase substance to be actually tested, the wavelengths of the light sources are predetermined, and the wavelengths of the two light sources cannot be changed during the use process of the liquid chromatography detector. For example, the wavelengths of the two light sources may be selected to be ultraviolet light and visible light, such as ultraviolet light at a first wavelength.
The light combining mirror 3 combines the two light sources and passes through the sample flow cell 4. As shown in fig. 1, the first wavelength light beam and the second wavelength light beam are perpendicular, and are distributed and irradiated on different surfaces of the combiner 3, and the first surface and the second surface refer to two different surfaces of the combiner 3. The combiner 3 is at an angle to the first wavelength beam, and the combiner 3 is also at an angle to the first wavelength beam, for example, may all be at 45 degrees. The light combining mirror 3 reflects the first wavelength light beam and transmits the second wavelength light beam so as to combine the two light sources, and the positions of the two light sources can be changed. The combined light beam after passing through the sample flow cell 4 is scattered, condensed by the condensing lens 5, and partially transmitted through the light transmission hole 6 and irradiated on the spectroscope 7. The beam splitter 7 transmits light of the first wavelength and light of the second wavelength separately. As shown in fig. 1, the beam splitter 7 is disposed at an angle, for example, 45 degrees, to the combined light beam, and reflects light of the first wavelength in the combined light beam and transmits light of the second wavelength, thereby dividing the combined light beam into a detection light beam of the first wavelength and a reference light beam of the second wavelength. The detection light detection module 8 detects the detection light beam with the first wavelength, outputs a corresponding detection light beam signal to the main control module, and the reference light detection module 9 detects the reference light beam with the second wavelength and outputs a reference light beam signal to the main control module.
Optionally, the detection light module 1 includes: a detection diode and a first focusing lens 11; the first wavelength light beam emitted by the detection diode is incident on the first surface of the light converging lens 3 through the first converging lens 11. The diode is used as a light source, so that the cost is low and the service life is long. The first converging lens 11 may be a spherical lens, or another converging lens may be used.
Optionally, the reference optical module 2 includes: a reference photodiode and a second converging lens 21; the second wavelength light beam emitted by the reference light diode is incident on the second surface of the light converging lens through the second converging lens 22. The second converging lens 21 may be a spherical lens, or may be another converging lens.
Alternatively, the converging lens 5 is a spherical lens, and other condensing lenses may be used. The purpose of the use of spherical lenses is to reduce the loss of light scattering outwards.
Optionally, the light passing holes 6 are arranged in a cross shape. After passing through the sample flow cell 4, the combined light beam needs to be focused by a light source and then propagates forward through a light passing hole 6. The light passing holes 6 are used for reducing the illumination reflected by the following light path. To achieve this, the light-passing hole may be narrowed, for example, in fig. 1, in a narrow cross shape.
Alternatively, the two sides of the light-passing hole 6 are made of materials or coatings with weak light reflection. Thereby reducing the interference of light reflection with detection.
Optionally, the detection beam detection module 8 includes a first optical filter 81 and a first photosensor 82;
the first photosensor 82 is configured to receive the detection beam passing through the first filter 81 and output the detection beam signal. Light with a first wavelength in the light path is reflected by the spectroscope 7, enters the first optical filter 81 and then is transmitted to the first photoelectric sensor 82; the optical signal is read by the main control module after being converted into an electric signal.
Optionally, the reference beam detection module 9 includes a second filter 91 and a second photosensor 92;
the second photosensor 92 is configured to receive the reference beam passing through the second filter 91 and output the reference beam signal. Light with the second wavelength in the light path directly enters the second optical filter 91 through the spectroscope 7 and then is transmitted to the second photoelectric sensor 92; the optical signal is read by the main control module after being converted into an electric signal. The optical filter filters the corresponding light source wavelength again to ensure the wavelength and bandwidth control of the light entering the photoelectric sensor.
As shown in fig. 2, the liquid chromatograph detector further includes a frame 10, the detection light module, the reference light module, the light converging lens, the light passing hole, the spectroscope, the detection light detection module, the reference light detection module and the main control module are disposed inside the frame 10, and the sample flow cell 4 is fixedly disposed outside the frame 10. For example, the sample flow cell 4 may be fixedly disposed on the top of the frame 10, and the sample flow cell may be directly installed and replaced outside the frame. The liquid chromatography detector can be powered by a direct current power supply, such as a 24V direct current power supply, and can be communicated with external equipment by a communication interface, such as an RS232 interface.
Optionally, there are three indicator lights outside the detector. The green light is on, the power supply is normal; the yellow light identifies that the detector is operating; the red light illumination identifies that the detector is faulty.
It should be noted that, in the above embodiment of the liquid chromatograph detector, each unit and module included are only divided according to the functional logic, but not limited to the above division, so long as the corresponding functions can be implemented; in addition, the specific names of the functional units are also only for distinguishing from each other, and are not used to limit the protection scope of the present utility model.
While the utility model has been described in detail in the foregoing general description, embodiments and experiments, it will be apparent to those skilled in the art that modifications and improvements can be made thereto. Accordingly, such modifications or improvements may be made without departing from the spirit of the utility model and are intended to be within the scope of the utility model as claimed.
Claims (10)
1. A liquid chromatography detector, comprising:
the detection light module is used for emitting a first wavelength light beam to the first surface of the light converging lens;
a reference light module for emitting a second wavelength light beam to a second surface of the light converging lens;
the first wavelength light beam and the second wavelength light beam are coaxially transmitted and irradiated to a sample flow cell arranged behind the light converging lens after being converged by the light converging lens;
a converging lens, a light-passing hole and a spectroscope are sequentially arranged behind the sample flow cell;
a detection light detection module for detecting the detection light beam of the first wavelength split by the spectroscope;
a reference light detection module for detecting a reference light beam of a second wavelength split by the beam splitter;
and the main control module is respectively connected with the detection light detection module and the reference light detection module and is used for determining a liquid chromatography detection result according to the reference light beam signal and the detection light beam signal.
2. The liquid chromatography detector of claim 1, wherein the detection light module comprises: a detection diode and a first focusing lens;
the first wavelength light beam emitted by the detection light diode is incident to the first surface of the light converging lens through the first converging lens.
3. The liquid chromatography detector of claim 2, wherein the reference optical module comprises: a reference photodiode and a second converging lens;
the second wavelength light beam emitted by the reference light diode is incident to the second surface of the light converging lens through the second converging lens.
4. The liquid chromatography detector of claim 1, wherein the converging lens is a spherical lens.
5. The liquid chromatography detector of claim 1, wherein the light passing holes are arranged in a cross shape.
6. The liquid chromatograph detector of claim 5, wherein the light-passing aperture is formed from a material or coating that is less reflective to light on both sides.
7. The liquid chromatography detector of claim 1, wherein the detection beam detection module comprises a first filter and a first photosensor;
the first photoelectric sensor is used for receiving the detection light beam passing through the first optical filter and outputting a detection light beam signal.
8. The liquid chromatography detector of claim 1, wherein the reference beam detection module comprises a second filter and a second photosensor;
the second photoelectric sensor is used for receiving the reference light beam passing through the second optical filter and outputting a signal of the reference light beam.
9. The liquid chromatograph detector of any of claims 1-8, further comprising a housing, wherein the detection light module, the reference light module, the combiner, the converging lens, the light passing aperture, the spectroscope, the detection light detection module, the reference light detection module, and the main control module are disposed inside the housing, and the sample flow cell is fixedly disposed outside the housing.
10. The liquid chromatography detector of claim 9, wherein the first wavelength is ultraviolet light.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321116575.0U CN219871188U (en) | 2023-05-10 | 2023-05-10 | Liquid chromatography detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321116575.0U CN219871188U (en) | 2023-05-10 | 2023-05-10 | Liquid chromatography detector |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219871188U true CN219871188U (en) | 2023-10-20 |
Family
ID=88370419
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321116575.0U Active CN219871188U (en) | 2023-05-10 | 2023-05-10 | Liquid chromatography detector |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219871188U (en) |
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2023
- 2023-05-10 CN CN202321116575.0U patent/CN219871188U/en active Active
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