CN113528312A - Convection current PCR amplification check out test set - Google Patents
Convection current PCR amplification check out test set Download PDFInfo
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- CN113528312A CN113528312A CN202110787092.2A CN202110787092A CN113528312A CN 113528312 A CN113528312 A CN 113528312A CN 202110787092 A CN202110787092 A CN 202110787092A CN 113528312 A CN113528312 A CN 113528312A
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- fixing frame
- lead screw
- pcr amplification
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- central heating
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- 238000012408 PCR amplification Methods 0.000 title claims abstract description 23
- 238000001514 detection method Methods 0.000 claims abstract description 38
- 239000013307 optical fiber Substances 0.000 claims abstract description 23
- 238000010438 heat treatment Methods 0.000 claims description 45
- 230000005284 excitation Effects 0.000 claims description 13
- 238000004321 preservation Methods 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 239000000463 material Substances 0.000 abstract description 4
- 230000003287 optical effect Effects 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 4
- 238000006243 chemical reaction Methods 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 230000003321 amplification Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- 108020004414 DNA Proteins 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 238000001917 fluorescence detection Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 150000007523 nucleic acids Chemical group 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- Proteomics, Peptides & Aminoacids (AREA)
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a convection PCR amplification detection device, which relates to the technical field of convection PCR amplification detection, and particularly relates to a convection PCR amplification detection device. In the convection PCR amplification detection device, exciting light emitted by an LED lamp passes through an exciting optical filter and is transmitted to a chip through an optical fiber to excite a fluorescent substance; the fluorescence emitted by the fluorescent material is conducted back to the detection module through the optical fiber and is transmitted to the detection module through the emission filter to collect and analyze data.
Description
Technical Field
The invention relates to the technical field of convection PCR amplification detection, in particular to a convection PCR amplification detection device.
Background
The polymerase chain reaction is called PCR for short, PCR is a method for synthesizing specific DNA fragments in vitro by enzyme, and consists of reactions of high-temperature denaturation, low-temperature annealing, proper-temperature extension and the like, wherein the reactions are performed in a cycle, so that the target DNA can be rapidly amplified, and the method has the characteristics of strong specificity, high sensitivity, simple and convenient operation, time saving and the like. It can be used for basic research of gene separation, cloning and nucleic acid sequence analysis, and also for diagnosis of diseases or any places with DNA and RNA. PCR is also known as cell-free molecular cloning or in vitro primer directed enzymatic amplification of specific DNA sequences.
The traditional PCR instrument has the defects of large volume, realization of three processes of PCR reaction by changing the temperature of an external environment and slow reaction.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a convection PCR amplification detection device, which solves the problems that the traditional PCR device has large volume, three processes of PCR reaction are realized by changing the external environment temperature, and the reaction is slow in the background technology.
In order to achieve the purpose, the invention is realized by the following technical scheme: the utility model provides a convection current PCR amplifications check out test set, includes the base, be equipped with on the base perpendicular and the slip table mount that is L font structure with the base to and be the optic fibre mount of zigzag structure, and arouse the detection module, be equipped with the slip table that is U font structure on the slip table mount, be equipped with lead screw sleeve post in the slip table, be equipped with the upper fixing frame that sets up with lead screw sleeve post axis is perpendicular on the lead screw sleeve post, the one end and the lead screw sleeve post fixed connection of upper fixing frame, the other end of upper fixing frame is equipped with the lower mounting frame with upper fixing frame parallel arrangement.
Optionally, wear to be equipped with accommodate the lead screw in the lead screw cover post, accommodate the lead screw's input is connected with step motor, step motor and slip table threaded connection, step motor is used for the height of accommodate the lead screw cover post through accommodate the lead screw.
Optionally, the heating patch is fixedly connected with the central heating body in an embedded manner, and the central heating body is fixedly connected with the upper fixing frame.
Optionally, a central heating body in a T-shaped structure is arranged between the upper fixing frame and the lower fixing frame, a heating paste is arranged at one end of the central heating body, a heat preservation layer is sleeved outside the other end of the central heating body, and a chip is arranged in the central heating body.
Optionally, one end of the lower fixing frame is movably connected with the central heating body, the other end of the lower fixing frame is provided with a rotating motor, the output end of the rotating motor is fixedly connected with the lower fixing frame, and one end, away from the lower fixing frame, of the rotating motor is sleeved with a motor fixing frame.
Optionally, the rotating electrical machine is fixedly connected with the motor fixing frame, and the motor fixing frame is fixedly connected with the base.
Optionally, an optical fiber is arranged on the optical fiber fixing frame in a penetrating manner, and the optical fiber is fixedly connected with the optical fiber fixing frame.
Optionally, an excitation filter, an emission filter, LED lamps arranged in one-to-one correspondence with the excitation filter, and detection modules arranged in one-to-one correspondence with the emission filter are arranged in the excitation detection module.
The invention provides a convection PCR amplification detection device, which has the following beneficial effects:
1. the heating paste arranged in the convection PCR amplification detection equipment is used for heating and conducting heat to the central heating body, and the central heating body is used for heating the middle part of the chip and enabling the center of the chip to be higher than a high-temperature area; meanwhile, the lower part of the heat-insulating layer is also provided with a heating body to keep the bottom and the periphery of the chip in a low-temperature area; the liquid in the high temperature area is heated to reduce the density and move upwards, and the liquid in the low temperature area moves downwards to form stable convection, so that the sample moves in the high and low temperature areas in a reciprocating manner, and the circulation of three temperature areas required by PCR reaction is realized, which is an amplification process.
2. In the convection PCR amplification detection device, exciting light emitted by an LED lamp passes through an exciting optical filter and is transmitted to a chip through an optical fiber to excite a fluorescent substance; the fluorescence emitted by the fluorescent material is conducted back to the detection module through the optical fiber and is transmitted to the detection module through the emission filter to collect and analyze data.
Drawings
FIG. 1 is a schematic perspective view of the present invention;
FIG. 2 is a schematic front view of the present invention;
FIG. 3 is a schematic cross-sectional view of an excitation detection module according to the present invention.
In the figure: 1. a central heating body; 2. a heating patch; 3. an upper fixing frame; 4. a chip; 5. a heat-insulating layer; 6. a lower fixing frame; 7. a rotating electric machine; 8. a motor fixing frame; 9. a sliding table fixing frame; 10. a sliding table; 11. an optical fiber; 12. an optical fiber holder; 13. a base; 14. an excitation detection module; 15. exciting the optical filter; 16. an LED lamp; 17. an emission filter; 18. a detection module.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, "a plurality" means two or more unless otherwise specified; the terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, should not be construed as limiting the invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "connected" and "connected" are to be interpreted broadly, e.g., as being fixed or detachable or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Referring to fig. 1 to 3, the present invention provides a technical solution: the utility model provides a convection current PCR amplifications check out test set, the on-line screen storage device comprises a base 13, be equipped with on base 13 perpendicular and the slip table mount 9 that is L font structure with base 13, and be the optic fibre mount 12 of zigzag structure, and arouse detection module 14, be equipped with the slip table 10 that is U font structure on the slip table mount 9, be equipped with lead screw sleeve post in the slip table 10, be equipped with on the lead screw sleeve post with the perpendicular upper mounting frame 3 that sets up of lead screw sleeve post axis, the one end and the lead screw sleeve post fixed connection of upper mounting frame 3, the other end of upper mounting frame 3 is equipped with the lower mounting frame 6 with 3 parallel arrangement of upper mounting frame.
In the invention: lead screw cover is worn to be equipped with accommodate the lead screw in the post, and accommodate the lead screw's input is connected with step motor, and step motor and slip table 10 threaded connection, step motor are used for the height of accommodate the lead screw cover post through accommodate the lead screw.
In the invention: the heating patch 2 is fixedly connected with the central heating body 1 in an embedded manner, and the central heating body 1 is fixedly connected with the upper fixing frame 3.
In the invention: a central heating body 1 in a T-shaped structure is arranged between the upper fixing frame 3 and the lower fixing frame 6, one end of the central heating body 1 is provided with a heating paste 2, the other end of the central heating body 1 is sleeved with a heat preservation layer 5, and a chip 4 is arranged in the central heating body 1; the heating paste 2 is heated and conducted to the central heating body 1, the central heating body 1 heats the middle part of the chip 4, and the center of the chip 4 is higher than the high-temperature area.
In the invention: one end of the lower fixing frame 6 is movably connected with the central heating body 1, the other end of the lower fixing frame 6 is provided with a rotating motor 7, the output end of the rotating motor 7 is fixedly connected with the lower fixing frame 6, and one end, far away from the lower fixing frame 6, of the rotating motor 7 is sleeved with a motor fixing frame 8.
In the invention: the rotating motor 7 is fixedly connected with the motor fixing frame 8, and the motor fixing frame 8 is fixedly connected with the base 13; the position of the chip 4 can be effectively changed by the cooperation of the rotating motor 7 and the lower fixing frame 6.
In the invention: an optical fiber 11 passes through the optical fiber fixing frame 12, and the optical fiber 11 is fixedly connected with the optical fiber fixing frame 12.
In the invention: an excitation filter 15, an emission filter 17, LED lamps 16 arranged in one-to-one correspondence to the excitation filter 15, and detection modules 18 arranged in one-to-one correspondence to the emission filter 17 are arranged in the excitation detection module 14; exciting light emitted by the LED lamp 16 passes through the excitation filter 15 and is transmitted to the chip 4 through the optical fiber 11 to excite the fluorescent substance; the fluorescence emitted by the fluorescent material is conducted back to the detection module 18 through the optical fiber 11, and transmitted to the detection module 18 through the emission filter 17 to be collected and analyzed.
In summary, when the convection PCR amplification detection device is used, firstly, the chip 4 is loaded, and the central heating body 1 is brought to the state shown in the figure by the cooperation of the rotating motor 7 and the lower fixing frame 6; the heating paste 2 is heated and conducted to the central heating body 1, the central heating body 1 heats the middle part of the chip 4, and the center of the chip 4 is arranged in a high-temperature area; meanwhile, the lower part of the heat-insulating layer 5 is also provided with a heating body to keep the bottom and the periphery of the chip 4 in a low-temperature area; the liquid in the high temperature area is heated to reduce the density and move upwards, and the liquid in the low temperature area moves downwards to form stable convection, so that the sample moves in the high and low temperature areas in a reciprocating manner, and the circulation of three temperature areas required by PCR reaction is realized, which is an amplification process;
then, the real-time fluorescence quantitative PCR needs to perform fluorescence detection on the reactant in the amplification process, wherein the fluorescence detection comprises that exciting light emitted by an LED lamp 16 passes through an excitation optical filter 15 and is transmitted to the chip 4 through an optical fiber 11 to excite a fluorescent substance; the fluorescence emitted by the fluorescent material is conducted back to the detection module 18 through the optical fiber 11, and transmitted to the detection module 18 through the emission filter 17 to be collected and analyzed.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical scope of the present invention and the equivalent alternatives or modifications according to the technical solution and the inventive concept of the present invention within the technical scope of the present invention.
Claims (8)
1. A convection current PCR amplifications check out test set, includes base (13), its characterized in that: be equipped with on base (13) perpendicular and be slip table mount (9) of L font structure with base (13) to and be optical fiber fixing frame (12) of Z font structure, and arouse detection module (14), be equipped with slip table (10) that are U font structure on slip table mount (9), be equipped with lead screw sleeve post in slip table (10), be equipped with on the lead screw sleeve post with perpendicular mount (3) that set up of lead screw sleeve post axis, the one end and the lead screw sleeve post fixed connection of mount (3), the other end of mount (3) is equipped with down mount (6) with mount (3) parallel arrangement.
2. The convective PCR amplification detection apparatus of claim 1, wherein: lead screw set posts wear to be equipped with accommodate the lead screw in, accommodate the lead screw's input is connected with step motor, step motor and slip table (10) threaded connection, step motor is used for the height of accommodate the lead screw set posts through accommodate the lead screw.
3. The convective PCR amplification detection apparatus of claim 1, wherein: the heating paste (2) is fixedly connected with the central heating body (1) in an embedded mode, and the central heating body (1) is fixedly connected with the upper fixing frame (3).
4. The convective PCR amplification detection apparatus of claim 1, wherein: the heating device is characterized in that a central heating body (1) in a T-shaped structure is arranged between the upper fixing frame (3) and the lower fixing frame (6), a heating paste (2) is arranged at one end of the central heating body (1), a heat preservation layer (5) is sleeved outside the other end of the central heating body (1), and a chip (4) is arranged in the central heating body (1).
5. The convective PCR amplification detection apparatus of claim 1, wherein: the heating device is characterized in that one end of the lower fixing frame (6) is movably connected with the central heating body (1), the other end of the lower fixing frame (6) is provided with a rotating motor (7), the output end of the rotating motor (7) is fixedly connected with the lower fixing frame (6), and one end, away from the lower fixing frame (6), of the rotating motor (7) is sleeved with a motor fixing frame (8).
6. The convective PCR amplification detection apparatus of claim 5, wherein: the rotating motor (7) is fixedly connected with the motor fixing frame (8), and the motor fixing frame (8) is fixedly connected with the base (13).
7. The convective PCR amplification detection apparatus of claim 1, wherein: the optical fiber fixing frame (12) is provided with an optical fiber (11) in a penetrating mode, and the optical fiber (11) is fixedly connected with the optical fiber fixing frame (12).
8. The convective PCR amplification detection apparatus of claim 1, wherein: and an excitation filter (15), an emission filter (17), LED lamps (16) which are arranged in a one-to-one corresponding mode with the excitation filter (15), and detection modules (18) which are arranged in a one-to-one corresponding mode with the emission filter (17) are arranged in the excitation detection module (14).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110787092.2A CN113528312A (en) | 2021-07-13 | 2021-07-13 | Convection current PCR amplification check out test set |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110787092.2A CN113528312A (en) | 2021-07-13 | 2021-07-13 | Convection current PCR amplification check out test set |
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| Publication Number | Publication Date |
|---|---|
| CN113528312A true CN113528312A (en) | 2021-10-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110787092.2A Pending CN113528312A (en) | 2021-07-13 | 2021-07-13 | Convection current PCR amplification check out test set |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105670924A (en) * | 2016-03-01 | 2016-06-15 | 上海理工大学 | Natural convection PCR (Polymerase Chain Reaction) microsystem |
| CN108342312A (en) * | 2017-01-24 | 2018-07-31 | 北京万泰生物药业股份有限公司 | Convection current PCR amplification detecting system and convection current PCR amplification detection method |
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2021
- 2021-07-13 CN CN202110787092.2A patent/CN113528312A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105670924A (en) * | 2016-03-01 | 2016-06-15 | 上海理工大学 | Natural convection PCR (Polymerase Chain Reaction) microsystem |
| CN108342312A (en) * | 2017-01-24 | 2018-07-31 | 北京万泰生物药业股份有限公司 | Convection current PCR amplification detecting system and convection current PCR amplification detection method |
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Application publication date: 20211022 |