CN205329008U - Real -time fluorescence quantitative PCR appearance - Google Patents
Real -time fluorescence quantitative PCR appearance Download PDFInfo
- Publication number
- CN205329008U CN205329008U CN201521132609.0U CN201521132609U CN205329008U CN 205329008 U CN205329008 U CN 205329008U CN 201521132609 U CN201521132609 U CN 201521132609U CN 205329008 U CN205329008 U CN 205329008U
- Authority
- CN
- China
- Prior art keywords
- temperature
- real
- module
- time fluorescence
- quantitative pcr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The utility model discloses a real -time fluorescence quantitative PCR appearance comprises basic PCR part, fluorescence detection part and host computer etc. PCR partially is the basis of this instrument, and including heating module, hot cap spare and temperature regulation module, compensation and heating through the temperature regulation module has the smooth completion of the basic demand of accurate temperature control, the quick PCR appearance such as temperature, temperature field homogeneous that go up and down, assurance PCR process. The fluorescence detection part is including swashing luminescent system and detecting system. The host computer includes data acquisition and system analysis software, and main being responsible for forms real -time figure from the next quick -witted data collection to carry out data processing and pattern analysis, the content that obtains target DNA passage and other examining report information etc..
Description
Technical field
This utility model relates to biochemical equipment field, specifically a kind of real-time fluorescence quantitative PCR instrument。
Background technology
PCR (polymerase chain reaction) refer to when utilizing the DNA high temperature of 95 DEG C in vitro can degeneration this strand can be combined by the principle of base pair complementarity with primer when becoming strand, low temperature about 60 DEG C, when temperature regulating to archaeal dna polymerase optimal reactive temperature about 72 DEG C, archaeal dna polymerase can character along phosphoric acid to the direction composition complementary strand of pentose (5 '-3 '), it is achieved the Protocols in Molecular Biology of the amplification specific DNA fragmentation of amplification。PCR instrument is then realize well controlling between denaturation temperature, renaturation temperature, elongating temperature, makes the equipment that the amplification of specific DNA is achieved。In order to monitor in real time course of reaction, and then adjust temperature in time, it is necessary to PCR instrument monitors target gene while temperature control。
Traditional PCR detection method is to judge whether target gene exists by amplified production is carried out gel electrophoresis, but there is many deficiencies in the method: owing to the efficiency of pcr amplification is significantly high, target gene is expanded to a large amount of from minute quantity and to be finally reached the saturated time shorter, electrophoresis detection can only obtain the qualitative results whether specific gene exists, and cannot be distinguished by said process, also its quantity or concentration quantitative cannot be detected, simultaneously, the method operating process is complicated, the detection time is long, the requirement of monitor in real time course of reaction cannot be met, additionally, owing to needing to be shifted by detection sample in operation, therefore sample is easily by the pollution of environment, in turn result in testing result inaccurate。
In order to solve the problems referred to above, occur in that Real-Time Fluorescent Quantitative PCR Technique, such as, Chinese patent literature CN101699271A discloses a kind of real-time fluorescence quantitative PCR excitation detection system, this system realizes the long fluorescence excitation of all-wave and detection based on Tunable filters, and this system includes the fluorescence excitation module being made up of light source, beam condensing unit and the first adjustable optical filter；The fluoroscopic examination module being made up of the second adjustable optical filter, photodetector and photoelectric conversion device；The control unit being made up of wavelength tuning equipment and main control computer；Respond test tube, Metal Temperature block and constant temp. heating lid composition heat circulating system。Said system is in DNA amplification reaction, by fluorescence signal, PCR is detected in real time, and through data process, target gene is carried out quantitative analysis, simple to operate, can automatically, quickly finish the detection by quantitative to target gene, and not by environmental pollution in detection process, testing result is comparatively accurate。But, above-mentioned real-time fluorescence quantitative PCR excitation detection system and at present other real-time fluorescence quantitative PCR instrument all there is also defect in temperature control: on the one hand, in a certain stage of reaction, when being maintained at a certain specific state of temperature, it is necessary to PCR instrument has good temperature uniformity；On the other hand, in monitor in real time course of reaction, it is necessary to machine adjusts temperature in time in due course, this further requirement PCR instrument can adjust rapidly temperature, and reaches the homogeneity of temperature in the short period of time；The temperature uniformity optimum of above-mentioned real-time fluorescence quantitative PCR excitation detection system and at present other real-time fluorescence quantitative PCR instrument is only capable of reaching ± 0.1 DEG C, although course of reaction namely can be detected in real time, but temperature control can not well be realized, and for PCR, reaction is the process of a dynamic competition, which product reaction temperature and time determine can be won, therefore, drawbacks described above result in it and can not realize the multiple analytic functions such as detection sample multisite mutation, single nucleotide polymorphism-SNP, gene type。
Utility model content
For this, the technical problems to be solved in the utility model is in that the defect overcoming real-time fluorescent quantitative PCR detection system of the prior art can not well realize the multiple analytic functions such as detection sample multisite mutation, single nucleotide polymorphism-SNP, gene type, this utility model patent provides a kind of real-time fluorescence quantitative PCR instrument, including basic PC R parts, fluoroscopic examination parts and host computer, described basic PC R parts include power-supply system and temperature control system；Described power-supply system includes power supply and power supply unit, and described power supply provides the electric energy of whole real-time fluorescence quantitative PCR instrument；Described power supply unit is combined power source adaptation plug connector, protects circuit；Described temperature control system includes heating module, hot cover and temperature adjustment module, and described temperature adjustment module is arranged at the periphery of sample base；Described heating module is cooling piece TE module, and described cooling piece TE module is arranged at the bottom of described sample base, is heated and lowers the temperature；Described hot cover is arranged at the top of described sample base, contacts with sample tube mouth, it is to avoid the steam coagulation that high temperature produces is on PCR pipe lid；Described sample base is that superconductor is made；The detection data that real-time fluorescence is changed by described host computer are analyzed processing；Described host computer is electrically connected to described control unit；Described control unit is electrically connected in TE temperature sensor, hot lid temperature sensor and environment temperature sensor；Described TE temperature sensor be arranged at described sample base bottom and and cooling piece TE module between, detect the temperature of described cooling piece TE module；Described hot lid temperature sensor is arranged on described hot cover, detects the temperature of described hot cover；Described environment temperature sensor is installed on the inside cavity of described real-time fluorescence quantitative PCR instrument, detects the cavity temperature of described real-time fluorescence quantitative PCR instrument；Described temperature adjustment module includes auxiliary heating module and radiating module, monitor described TE temperature sensor, hot lid temperature sensor and environment temperature sensor by control unit and adjust the homogeneity of PCR reaction temperature less than ± 0.1 DEG C, lifting temperature Mean Speed is more than 3 DEG C/sec, and temperature accuracy controls less than ± 0.15 DEG C；Described fluoroscopic examination parts include: electro-optical system, and sample carries out the detection of real-time fluorescence change；Described electro-optical system is by including exciting light system and detection system；Described exciting light system includes Halogen light and optical filter；Described detection system includes optical fiber, emission filter, camera lens and CCD camera；Fluorescence signal passes through described optical fiber, emission filter and camera lens, arrives described CCD camera。
Further, described control unit includes master board and sub-control plate；The port of described master control plate-making is connected to described power-supply system, temperature control system, electro-optical system；Described master board controls the temperature having the temperature of described cooling piece TE module, hot cover, the temperature of auxiliary heating module and the radiating module temperature to real-time fluorescence quantitative PCR instrument cavity；The port of described master board is connected to the first selection motor and second controlling optical filter and selects motor；The port of described sub-control plate is connected to up and down motion the first direct current generator and the second direct current generator that control instrument top cover and the limit switch of four groups of control signal lamp switches。
Further, described radiating module is two intelligent radiator fans。
Further, described auxiliary heating module and radiating module, the homogeneity being adjusted PCR reaction temperature by the application software of described host computer is ± 0.06 DEG C。
Further, described auxiliary heating module is electric heating tube or heating tape。
Further, heated module is six groups of independent control cooling piece TE modules, is connected with six groups of TE temperature sensors respectively。
Further, the temperature of described hot cover is 105 DEG C。
Further, described radiating module is two intelligent radiator fans, controls the inside cavity temperature of described real-time fluorescence quantitative PCR instrument below 45 DEG C。
Further, described exciting light system is excited by optical fiber 96 hole simultaneously；Described detection system includes a unjacketed optical fiber, four transmitting optical filter wheels, one group of eyeglass and cooled ccd camera；The wave-length coverage of fluoroscopic examination is 380nm to 780nm, and excitation wavelength port number is set to 1 to 8, and transmitted wave length is set to 1 to 5 kind accordingly。
Beneficial effect:
A kind of quantitative real time PCR Instrument that this utility model provides utilizes control circuit while target gene is expanded by control thermocirculator, the fluorescence signal intensity of tested reactant to be detected, owing to fluorescence signal intensity and the concentration of target gene are proportional relations, therefore fluorescence intensity just can know that quantity and the concentration of specific gene。Therefore the detection time of clinical bacteria is substantially reduced。Additionally, this quantitative fluorescent PCR is made temperature uniformity be rapidly achieved by auxiliary heating module, radiating module and the sample base being made up of superconductor and is maintained at ± 0.06 DEG C for a long time, achieve amplicon sequence analysis (ASA, AmpliconSequenceAnalysis) function, ASA is not limited to by mutating alkali yl site and type, without sequence-specific probes, after PCR terminates, directly run high-resolution melt, got final product paired samples sudden change, single nucleotide polymorphism-SNP, methylate, the analysis of HLA distribution type etc.。
Meanwhile, PCR thermal cycle, fluoroscopic examination and the computer structure equipped with various analysis softwares are combined by this utility model, it is possible to dynamically observing the situation that in the PCR each reaction tube of each circulation, pcr amplification product is gradually increased, simple to operate, response speed is fast。
Accompanying drawing explanation
Accompanying drawing is used for providing being further appreciated by of the present utility model, and constitutes a part for description, is used for explaining this utility model, is not intended that restriction of the present utility model together with embodiment of the present utility model。In the accompanying drawings:
Fig. 1 is each parts annexation schematic diagram of this utility model a kind of real-time fluorescence quantitative PCR instrument；
Fig. 2 is the structural representation of the temperature control system of a kind of real-time fluorescence quantitative PCR instrument of this utility model；
Fig. 3 is the electric appliance circuits schematic diagram of a kind of real-time fluorescence quantitative PCR instrument of this utility model；
In figure, accompanying drawing labelling is expressed as: 10-master board；The hot cover of 11-；The hot lid temperature sensor of 12-；13-radiating module；14-environment temperature sensor；15-first selects motor；16-second selects motor；20-sample base；21-cooling piece TE module；22-TE temperature sensor；23-heating tape；30-sub-control plate；31-the first direct current generator；32-the second direct current generator；33-limit switch。
Detailed description of the invention
Below according to drawings and Examples, this utility model is described in further detail。
As it is shown in figure 1,
A kind of real-time fluorescence quantitative PCR instrument, including power supply, power supply unit, heating module, hot cover, temperature adjustment module, electro-optical system, control unit and host computer。
Host computer is analyzed processing by the detection data that real-time fluorescence is changed by application software；Control unit to heating module, hot cover, temperature adjustment module and electro-optical system intelligent monitoring。
As in figure 2 it is shown,
Temperature adjustment module includes auxiliary heating module, and auxiliary heating module is heating tape 23, for conductive plastics material, is arranged at the periphery of sample base 20, it is provided that compensate temperature；Heating module is cooling piece TE module 21, and the one side of described cooling piece TE module 21 can heat, and one side can cool down, and has heating and the effect of cooling, is arranged at the bottom of described sample base 20, for sample being heated and lowering the temperature；TE temperature sensor 22 is arranged at sample base 20 and heating module is between cooling piece TE module 21；Sample base 20 is superconductor。
As it is shown on figure 3,
The electric appliance circuits figure of real-time fluorescence quantitative PCR instrument is made up of master board 10 and sub-control plate 30；The port of master control plate-making is connected to described power-supply system, temperature control system, electro-optical system；Master board 10 controls to have the temperature of the temperature of described six groups of cooling piece TE modules 21, hot cover, the temperature of auxiliary heating module and two the intelligent radiator fans temperature to real-time fluorescence quantitative PCR instrument cavity；The port of master board 10 is connected to control optical filter first and selects motor 15 and second to select motor 16；The port of sub-control plate 30 is connected to up and down motion the first direct current generator 31 and the second direct current generator 32 that control instrument top cover and the limit switch 33 of four groups of control signal lamp switches。
The using method of a kind of real-time fluorescence quantitative PCR instrument described in the present embodiment is as follows:
As Figure 1-3,
A kind of real-time fluorescence quantitative PCR instrument includes basic PC R parts, fluoroscopic examination parts and host computer。Basic PC R parts are the bases of this instrument, the part such as including power supply, heating module, temperature acquisition and process, and it must have the basic demand of all first-class PCR instrument in accurate temperature controlling, rapid temperature rise and drop, temperature field, it is ensured that smoothly completing of PCR process。Fluoroscopic examination parts include the parts such as light source, photomultiplier tube, Signal acquiring and processing。Host computer includes data acquisition and systematic analysis software, and primary responsibility gathers data from slave computer, forms real-time graph, and carries out data process and pattern analysis, obtains content and other examining report information etc. of target DNA fragments。During work, first master computer puts into operation, staff puts into institute's test sample product on the specimen holder of PCR reflection cavity simultaneously, staff arranges corresponding temperature parameter according to the PCR reaction condition of institute's test sample product, temperature control time and cycle-index etc., the personnel of proceeding with one's work click corresponding button control, system is put into being rapidly heated, constant temperature, fast cooling, the PCR cycle processes such as constant temperature, until all of loop ends, before the low-temperature zone constant temperature of each circulation ties card, system carries out the fluorescence signal Real-time Collection of sample simultaneously, master computer forms real-time graph according to the signal collected, and carry out data process and pattern analysis, obtain content and other examining report information etc. of target DNA fragments。
Application software of the present utility model has been arranged on host computer, runs, it is achieved Setup Experiments, data management and other functions under Windows operating system。The self-inspection of application software will check temperature control system and electro-optical system, and the temperature of cooling piece TE module 21, hot cover 11 and real-time fluorescence quantitative PCR instrument cavity is monitored respectively by master board 10 by TE temperature sensor 22, hot lid temperature sensor 12 and environment temperature sensor 14。
Master board 10 can control heating module, the heating of auxiliary heating module and cooling, master board 10 receives the temperature signal that TE temperature sensor 22, hot lid temperature sensor 12 and environment temperature sensor 14 detect, due to described TE temperature sensor 22 be arranged at described sample base bottom and and cooling piece TE module 21 between, it is possible to detect the temperature of described cooling piece TE module 21；Described hot lid temperature sensor 12 is arranged on described hot cover 11, detects the temperature of described hot cover 11；Described environment temperature sensor 14 is installed on the inside cavity of described real-time fluorescence quantitative PCR instrument, detects the cavity temperature of described real-time fluorescence quantitative PCR instrument。So, master board 10 is obtained with the cavity temperature of the temperature of cooling piece TE module 21, the temperature of hot cover 11, PCR instrument, compares according to the temperature that the Current Temperatures detected and needs reach, heating module, auxiliary heating module are controlled
Master board 10 is connected with TE temperature sensor 22, adjusts the temperature transition in each stages such as denaturation stage, renaturation stage and extension stages of real-time fluorescence quantitative PCR instrument, accelerates heating and cooling。
At denaturation stage, sample support 20 is heated by cooling piece TE module 21 and heating tape 23。Due to, sample base 20 is superconductor, it is possible to transfer thermal energy to rapidly sample tube so that it is rapidly to about 95 DEG C, thus reaching the effect being rapidly heated。Until time too high or too low for temperature, signal is passed to master board 10 by TE temperature sensor 22, thus adjusting cooling piece TE module 21 and heating tape 23 heats or lowers the temperature。
In the renaturation stage, it is necessary to be annealed to 50～60 DEG C, cooling piece TE module 21 and heating tape 23 stop heating, and meanwhile, two intelligent radiator fans of radiating module 13 are started working；After set point of temperature, signal is passed to master board 10 by TE temperature sensor 22, controls cooling piece TE module 21 and heating tape 23 keeps identical temperature, stop two intelligent radiator fan works。
Extending the stage, needing heating to 75 DEG C, sample support 20 is heated by cooling piece TE module 21 and heating tape 23, until time too high or too low for temperature, signal is passed to master board 10 by TE temperature sensor 22, thus adjusting cooling piece TE module 21 and heating tape 23 heats or lowers the temperature。
So when each stage specified temp reacts, being heated by the compensation of heating tape 23 so that the homogeneity of reaction temperature is maintained at ± 0.06 DEG C for a long time, lifting temperature Mean Speed is 3 DEG C/sec。
It addition, the temperature that master board 10 controls hot cover 11 by hot lid temperature sensor 12 is 105 DEG C, when variations in temperature, it is possible to be corrected by the signal of telecommunication in time。And control the cavity temperature of real-time fluorescence quantitative PCR instrument by environment temperature sensor 14 and be maintained at 35 DEG C。Meanwhile, master board 10 port connection control optical filter first selects motor 15 and second to select motor 16 to select different optical filter。
Light source used by fluoroscopic examination parts is Halogen light, is excited by optical fiber 96 hole simultaneously；Detection system includes a unjacketed optical fiber, four transmitting optical filter wheels, one group of eyeglass and cooled ccd camera；The wave-length coverage of fluoroscopic examination is 380nm to 780nm, and excitation wavelength port number is 8, launches wavelength 5 kinds。
Sub-control plate 30 port is connected to up and down motion the first direct current generator 31 and the second direct current generator 32 that control instrument top cover and the limit switch 33 of four groups of control signal lamp switches, thus realizing external physical motion and the control of each functional switch of this instrument。
The foregoing is only explanation embodiment of the present utility model; it is not limited to this utility model; for a person skilled in the art; all within spirit of the present utility model and principle; any amendment of being made, equivalent replacement, improvement etc., should be included within protection domain of the present utility model。
Claims (9)
1. a real-time fluorescence quantitative PCR instrument, including basic PC R parts, fluoroscopic examination parts and host computer, it is characterised in that
Described basic PC R parts include power-supply system and temperature control system；Described power-supply system includes power supply and power supply unit, and described power supply provides the electric energy of whole real-time fluorescence quantitative PCR instrument；Described power supply unit is combined power source adaptation plug connector, protects circuit；
Described temperature control system includes heating module, hot cover and temperature adjustment module, and described temperature adjustment module is arranged at the periphery of sample base；Described heating module is cooling piece TE module, and described cooling piece TE module is arranged at the bottom of described sample base, is heated and lowers the temperature；Described hot cover is arranged at the top of described sample base, contacts with sample tube mouth；Described sample base is that superconductor is made；
The detection data that real-time fluorescence is changed by described host computer are analyzed processing；Described host computer is electrically connected to control unit；Described control unit is electrically connected in TE temperature sensor, hot lid temperature sensor and environment temperature sensor；Described TE temperature sensor is arranged between the bottom of described sample base and cooling piece TE module, detects the temperature of described cooling piece TE module；Described hot lid temperature sensor is arranged on described hot cover, detects the temperature of described hot cover；Described environment temperature sensor is installed on the inside cavity of described real-time fluorescence quantitative PCR instrument, detects the cavity temperature of described real-time fluorescence quantitative PCR instrument；
Described temperature adjustment module includes auxiliary heating module and radiating module, monitor described TE temperature sensor, hot lid temperature sensor and environment temperature sensor by control unit and adjust the homogeneity of PCR reaction temperature less than ± 0.1 DEG C, lifting temperature Mean Speed is more than 3 DEG C/sec, and temperature accuracy controls less than ± 0.15 DEG C；
Described fluoroscopic examination parts include: electro-optical system, and sample carries out the detection of real-time fluorescence change；Described electro-optical system is by including exciting light system and detection system；Described exciting light system includes Halogen light and optical filter；Described detection system includes optical fiber, emission filter, camera lens and CCD camera；Fluorescence signal passes through described optical fiber, emission filter and camera lens, arrives described CCD camera。
2. real-time fluorescence quantitative PCR instrument according to claim 1, it is characterised in that described control unit includes master board and sub-control plate；
The port of described master control plate-making is connected to described power-supply system, temperature control system, electro-optical system；
Described master board controls the temperature having the temperature of described cooling piece TE module, hot cover, the temperature of auxiliary heating module and the radiating module temperature to real-time fluorescence quantitative PCR instrument cavity；
The port of described master board is connected to the first selection motor and second controlling optical filter and selects motor；
The port of described sub-control plate is connected to up and down motion the first direct current generator and the second direct current generator that control instrument top cover and the limit switch of four groups of control signal lamp switches。
3. the real-time fluorescence quantitative PCR instrument according to any one of claim 2, it is characterised in that described radiating module is two intelligent radiator fans。
4. the real-time fluorescence quantitative PCR instrument according to any one of claim 1 or 2, it is characterised in that described auxiliary heating module and radiating module, the homogeneity being adjusted PCR reaction temperature by the application software of described host computer is ± 0.06 DEG C。
5. the real-time fluorescence quantitative PCR instrument according to any one of claim 1 or 2, it is characterised in that described auxiliary heating module is electric heating tube or heating tape。
6. the real-time fluorescence quantitative PCR instrument according to any one of claim 1 or 2, it is characterised in that described radiating module is two intelligent radiator fans, controls the inside cavity temperature of described real-time fluorescence quantitative PCR instrument below 45 DEG C。
7. the real-time fluorescence quantitative PCR instrument according to any one of claim 1 or 2, it is characterised in that heated module is six groups of independent control cooling piece TE modules, is connected with six groups of TE temperature sensors respectively。
8. the real-time fluorescence quantitative PCR instrument according to any one of claim 1 or 2, it is characterised in that the temperature of described hot cover is 105 DEG C。
9. real-time fluorescence quantitative PCR instrument according to claim 1, it is characterised in that described exciting light system is excited by optical fiber 96 hole simultaneously；Described detection system includes a unjacketed optical fiber, four transmitting optical filter wheels, one group of eyeglass and cooled ccd camera；The wave-length coverage of fluoroscopic examination is 380nm to 780nm, and excitation wavelength port number is set to 1 to 8, and transmitted wave length is set to 1 to 5 kind accordingly。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521132609.0U CN205329008U (en) | 2015-12-31 | 2015-12-31 | Real -time fluorescence quantitative PCR appearance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521132609.0U CN205329008U (en) | 2015-12-31 | 2015-12-31 | Real -time fluorescence quantitative PCR appearance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205329008U true CN205329008U (en) | 2016-06-22 |
Family
ID=56312862
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201521132609.0U Active CN205329008U (en) | 2015-12-31 | 2015-12-31 | Real -time fluorescence quantitative PCR appearance |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205329008U (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106047688A (en) * | 2016-07-29 | 2016-10-26 | 车团结 | PCR (polymerase chain reaction) instrument and temperature control system for same |
| CN106497777A (en) * | 2016-09-22 | 2017-03-15 | 苏州百源基因技术有限公司 | A kind of self-checking system of real-time fluorescence quantitative PCR instrument and its self checking method |
| CN106872432A (en) * | 2017-03-30 | 2017-06-20 | 杭州晶格科学仪器有限公司 | A kind of fluorescence detection device and temperature control method with temperature control mechanism |
| CN107043697A (en) * | 2017-06-14 | 2017-08-15 | 北京百康芯生物科技有限公司 | A kind of multi-channel chip constant-temperature amplification detection of nucleic acids instrument |
| CN107083426A (en) * | 2017-03-30 | 2017-08-22 | 杭州晶格科学仪器有限公司 | A kind of fluorescence quantitative detecting method |
| CN108107024A (en) * | 2016-11-25 | 2018-06-01 | 苏州百源基因技术有限公司 | A kind of intelligence PCR instrument |
| CN108192997A (en) * | 2016-12-08 | 2018-06-22 | 苏州百源基因技术有限公司 | A kind of temprature control method and device of PCR instrument orifice plate subregion |
| CN111139179A (en) * | 2019-12-26 | 2020-05-12 | 中国计量大学 | Portable isothermal amplification rapid detector capable of independently controlling temperature |
| CN111304051A (en) * | 2020-02-20 | 2020-06-19 | 珠海黑马生物科技有限公司 | PCR instrument and use method |
| CN112823890A (en) * | 2020-07-30 | 2021-05-21 | 北京金诺美生物技术有限公司 | Real-time fluorescence quantitative PCR instrument |
| CN114015561A (en) * | 2021-11-05 | 2022-02-08 | 中元汇吉生物技术股份有限公司 | PCR fluorescence detection temperature control system |
-
2015
- 2015-12-31 CN CN201521132609.0U patent/CN205329008U/en active Active
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106047688A (en) * | 2016-07-29 | 2016-10-26 | 车团结 | PCR (polymerase chain reaction) instrument and temperature control system for same |
| CN106497777A (en) * | 2016-09-22 | 2017-03-15 | 苏州百源基因技术有限公司 | A kind of self-checking system of real-time fluorescence quantitative PCR instrument and its self checking method |
| CN108107024A (en) * | 2016-11-25 | 2018-06-01 | 苏州百源基因技术有限公司 | A kind of intelligence PCR instrument |
| CN108192997A (en) * | 2016-12-08 | 2018-06-22 | 苏州百源基因技术有限公司 | A kind of temprature control method and device of PCR instrument orifice plate subregion |
| CN106872432A (en) * | 2017-03-30 | 2017-06-20 | 杭州晶格科学仪器有限公司 | A kind of fluorescence detection device and temperature control method with temperature control mechanism |
| CN107083426A (en) * | 2017-03-30 | 2017-08-22 | 杭州晶格科学仪器有限公司 | A kind of fluorescence quantitative detecting method |
| CN107043697A (en) * | 2017-06-14 | 2017-08-15 | 北京百康芯生物科技有限公司 | A kind of multi-channel chip constant-temperature amplification detection of nucleic acids instrument |
| CN111139179A (en) * | 2019-12-26 | 2020-05-12 | 中国计量大学 | Portable isothermal amplification rapid detector capable of independently controlling temperature |
| CN111139179B (en) * | 2019-12-26 | 2023-03-14 | 中国计量大学 | Portable isothermal amplification rapid detector capable of independently controlling temperature |
| CN111304051A (en) * | 2020-02-20 | 2020-06-19 | 珠海黑马生物科技有限公司 | PCR instrument and use method |
| CN111304051B (en) * | 2020-02-20 | 2023-08-11 | 珠海黑马生物科技有限公司 | PCR instrument and use method thereof |
| CN112823890A (en) * | 2020-07-30 | 2021-05-21 | 北京金诺美生物技术有限公司 | Real-time fluorescence quantitative PCR instrument |
| CN114015561A (en) * | 2021-11-05 | 2022-02-08 | 中元汇吉生物技术股份有限公司 | PCR fluorescence detection temperature control system |
| CN114015561B (en) * | 2021-11-05 | 2023-11-14 | 中元汇吉生物技术股份有限公司 | PCR fluorescent detection temperature control system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN205329008U (en) | Real -time fluorescence quantitative PCR appearance | |
| CN106929388A (en) | A kind of real-time fluorescence quantitative PCR instrument | |
| CN100567962C (en) | A kind of hot air circulating temperature controlling real-time fluorescent quantitative PCR detector | |
| CA2591550C (en) | Monitoring hybridization during pcr | |
| CN103820316B (en) | Based on the real-time PCR detection system of rotary micro-fluidic chip | |
| KR102246869B1 (en) | Polymerase chain reaction detection mechanism and polymerase chain reaction device | |
| CN106140338B (en) | The method of micro-fluidic chip system and the application system progress isothermal duplication and detection | |
| TWI715997B (en) | Pcr apparatus for real-time detecting multiplex fluorescent signals | |
| CN105802848A (en) | Recombinase-mediated isothermal nucleic acid amplification reaction real-time detection apparatus | |
| CN103173434A (en) | Method and device for carrying out polymerase chain reaction under constant-temperature heat source | |
| CN104130933A (en) | Thermostatic fluorescence PCR (polymerase chain reaction) amplifier | |
| CN106461554A (en) | Apparatus and method for thermocyclic biochemical operations | |
| CN108034703A (en) | Digital pcr system based on EWOD drivings and constant temperature source | |
| JP2003344290A (en) | Fluorescence detector with temperature controller | |
| CN104120077A (en) | Real-time fluorescent DNA amplification instrument | |
| CN107653187A (en) | Random pcr system | |
| CN106497777A (en) | A kind of self-checking system of real-time fluorescence quantitative PCR instrument and its self checking method | |
| CN104120078A (en) | Real-time fluorescence PCR (Polymerase Chain Reaction) amplification instrument | |
| JP2011072263A (en) | Gene analyzer | |
| CN111443213B (en) | Multi-variable reaction dynamics real-time detector device and detection method | |
| CN2775652Y (en) | Multiple PCR quantitative detector | |
| CN111154623A (en) | Portable convection PCR amplification detection device with vibration function | |
| CN113969238A (en) | Portable visual imaging system for gene amplification fluorescence detection | |
| CN103732757A (en) | Thermal convection polymerase chain reaction device | |
| CN206057068U (en) | A kind of sample ashing device of fast heating intelligent temperature control |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |