CN113521165A - Gynecological external antibacterial gel and preparation method thereof - Google Patents
Gynecological external antibacterial gel and preparation method thereof Download PDFInfo
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Abstract
The invention relates to the technical field of traditional Chinese medicines, in particular to a gynecological external antibacterial gel and a preparation method thereof. The gynecological external antibacterial gel comprises herba patriniae, ephedra, radix clematidis, eucalyptus oil, sodium benzoate, glycerin, carbomer, polysorbate 80 and triethanolamine; wherein the mass ratio of the patrinia, the ephedra and the clematis root is 2:2: 1. The gynecological external antibacterial gel disclosed by the invention has a strong inhibiting effect on pathogenic microorganisms such as staphylococcus aureus, escherichia coli, candida albicans and the like, has obvious anti-inflammatory and bactericidal effects, is quick in itching relieving, has no stimulation to skin and mucosa, is suitable for cleaning and nursing superficial wound surfaces and skin around the wound surfaces of gynecological vaginas, and is suitable for daily nursing of the gynecological vaginas.
Description
Technical Field
The invention belongs to the technical field of medicines, and particularly relates to a gynecological external antibacterial gel and a preparation method thereof.
Background
Gynecological inflammation and pruritus vulvae always are a large disease bothering women, are a symptom caused by various gynecological diseases, have high recurrence rate, need long-time medication, bring unmanageable worries to female patients and seriously affect the lives of the female patients. According to the epidemiological survey results in China, the prevalence rate of gynecological diseases of married women in China reaches over 67 percent at present, and the women are in an increasing trend year by year.
The existing medicines for treating gynecological inflammation and pruritus vulvae comprise two categories of external medicines and oral medicines, along with the continuous development of medical technology, the research on antibacterial medicines is continuous and deep, the gynecological external gel preparation has good biological adhesion and biocompatibility, the medicines can be adhered to vaginal mucosa, the action time of the medicines is further prolonged, meanwhile, the gel has the advantages of unique lubricity, cool feeling, convenience in use and the like, and the gel is popular and is a hotspot for the research on gynecological external medicines in the future.
The gynecological gel at present mainly comprises traditional Chinese medicine and western medicine gels, the traditional Chinese medicine gel is poor in antibacterial performance, and meanwhile, skin itching is easily caused, vaginal mucosa is stimulated, and the use comfort is poor. The western medicine gel is easy to generate drug resistance, easy to recur and the like.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the gynecological external antibacterial gel which has excellent antibacterial performance and quick itching relieving effect and does not generate irritation influence on vaginal mucosa and skin.
The gynecological external antibacterial gel comprises the following components:
herba Patriniae, herba Ephedrae, radix Clematidis, oleum Eucalypti, sodium benzoate, glycerol, carbomer, polysorbate 80, and triethanolamine; the mass ratio of the patrinia, the ephedra and the clematis root is 2:2: 1.
Preferably, the eucalyptus oil is obtained by distilling and extracting fresh leaves and stems of eucalyptus lobular swamp, and the essential oil contains 1, 8-cineole as a main component.
Preferably, the gynecological external antibacterial gel comprises the following components in parts by weight:
patrinia 20g
Ephedra, herba Ephedrae 20g
Clematis root 10g
Eucalyptus oil 20g
Sodium benzoate 7.5g
80-100g of glycerol
Carbomer 8-10g
Polysorbate 8020g
3-10g of triethanolamine.
The invention also provides a preparation method of the gynecological external antibacterial gel, which comprises the following steps:
1) weighing herba Patriniae, herba Ephedrae and radix Clematidis at a certain proportion, and distilling herba Patriniae to obtain herba Patriniae distillate; then adding herba Ephedrae and radix Clematidis into the residue of herba Patriniae herb after distillation, decocting with water, concentrating to obtain fluid extract of 1.05-1.10, adding sodium benzoate, mixing, centrifuging, and filtering to obtain Chinese medicinal extract;
2) mixing the traditional Chinese medicine extract obtained in the step 1) and the patrinia distillation liquid to obtain a mixed liquid I;
3) distilling fresh leaves of Eucalyptus parviflora to obtain oleum Eucalypti;
4) fully swelling carbomer in purified water, and stirring to obtain carbomer matrix for later use;
5) adding eucalyptus oil, glycerin and polysorbate 80 into the mixed solution I in proportion, and uniformly mixing to obtain a mixed solution II;
6) and (3) adding the mixed solution II obtained in the step 5) into a carbomer matrix, uniformly stirring, adjusting the pH to 5-6 by using triethanolamine, adding a proper amount of purified water to enable the total mass to reach the specified prescription amount, uniformly stirring, and preparing the gynecological external antibacterial gel.
Preferably, the patrinia distillation liquid in the step 1) is prepared by crushing patrinia and then distilling the crushed patrinia for 1 to 2 hours under normal pressure.
Preferably, the traditional Chinese medicine extract is prepared by mixing herba patriniae dregs, ephedra herb and radix clematidis in proportion, adding 8 times of water of the total mass of the medicinal materials, carrying out vacuum decoction for 1-2 hours under reduced pressure, taking decoction, adding 6 times of water of the total mass of the medicinal materials into the dregs, carrying out vacuum decoction for 30-60 min under reduced pressure, combining the decoction, filtering, and concentrating to obtain clear paste with the relative density of 1.05-1.10.
Preferably, the patrinia distillation liquid is prepared by drying patrinia in the shade, crushing into coarse powder, distilling at normal pressure for 1-2h to prepare primary distillation liquid, and redistilling the primary distillation liquid to prepare the patrinia distillation liquid.
Preferably, the eucalyptus oil in the step 3) is obtained by taking fresh leaves and stems of eucalyptus lobular swamp, crushing into coarse powder, and distilling under normal pressure to extract essential oil.
Compared with the prior art, the invention has the following beneficial effects:
the invention has unique prescription and novel preparation method, extracts the volatile active ingredients with stronger antibiosis and antiphlogosis in the patrinia by a distillation method for treating gynecological diseases, and is matched with the components of the patrinia, the ephedra, the water-soluble active ingredients of the clematis root, the eucalyptus oil and the like, because of the antibacterial synergistic action among the components, the antibacterial, the insect-inhibiting and antiphlogistic effects of the invention are enhanced, and the gel preparation is prepared simultaneously, so that the medicine can be adhered to mucous membranes and wound surfaces for a long time, the medicine forms a protective film and a nursing barrier on the surface of the wound surfaces, the medicine has lasting and mild effects, the recurrence rate is low, and the gel preparation has no irritation to the skin and the vaginal mucous membranes, improves the use comfort level and can meet the actual requirements of patients.
Detailed Description
The present invention will be further described with reference to the following specific examples.
Example 1
A preparation method of gynecological external antibacterial gel comprises:
1) weighing 20g of herba patriniae, 20g of ephedra herb and 10g of radix clematidis, crushing the herba patriniae into coarse powder, and preparing herba patriniae distillate by a distillation method for later use; then adding herba Ephedrae and radix Clematidis into the herba Patriniae residue obtained after distillation, mixing, adding 8 times of water of the total weight of the medicinal materials, vacuum decocting for 1 hr under reduced pressure, collecting decoction, adding 6 times of water of the total weight of the medicinal materials into the residue, vacuum decocting for 50min under reduced pressure, mixing decoctions, filtering, concentrating to obtain fluid extract with relative density of 1.05, adding 7.5g sodium benzoate, mixing, centrifuging, filtering, and preparing Chinese medicinal extract for use; wherein the herba Patriniae distillate is prepared by drying herba Patriniae in the shade, pulverizing into coarse powder, distilling under normal pressure for 1.5 hr, collecting primary distillate, and redistilling to obtain herba Patriniae distillate;
2) mixing the traditional Chinese medicine extract obtained in the step 1) and the patrinia distillation liquid to obtain a mixed liquid I;
3) distilling fresh leaves and stems of Eucalyptus globulus Labill to obtain oleum Eucalypti, wherein the essential oil contains 1, 8-cineole as main ingredient;
4) fully swelling 8g of carbomer in purified water, and uniformly stirring to prepare a carbomer matrix for later use;
5) adding 20g of eucalyptus oil, 90g of glycerol and 20g of polysorbate 80 into the mixed solution I, and uniformly mixing to obtain a mixed solution II;
6) and (3) adding the mixed solution II obtained in the step 5) into a carbomer matrix, uniformly stirring, adding 10g of triethanolamine, adding an appropriate amount of purified water to ensure that the total mass is 1000g, and uniformly stirring to prepare the gynecological external antibacterial gel.
Example 2
A preparation method of gynecological external antibacterial gel comprises:
1) weighing 20g of herba patriniae, 20g of ephedra herb and 10g of radix clematidis, and preparing herba patriniae distillate by the herba patriniae with a distillation method for later use; then adding herba Ephedrae and radix Clematidis into the herba Patriniae residue obtained after distillation, mixing, adding 8 times of water of the total weight of the medicinal materials, vacuum decocting under reduced pressure for 2 hr, collecting decoction, adding 6 times of water of the total weight of the medicinal materials into the residue, vacuum decocting under reduced pressure for 40min, mixing decoctions, filtering, concentrating to obtain fluid extract with relative density of 1.10, adding 7.5g sodium benzoate, mixing, centrifuging, filtering, and preparing Chinese medicinal extract; wherein the herba Patriniae distillate is prepared by drying herba Patriniae in the shade, pulverizing into coarse powder, distilling under normal pressure for 2 hr, collecting primary distillate, and redistilling to obtain herba Patriniae distillate;
2) mixing the traditional Chinese medicine extract obtained in the step 1) and the patrinia distillation liquid to obtain a mixed liquid I;
3) distilling fresh leaves and stems of Eucalyptus globulus Labill to obtain oleum Eucalypti, wherein the essential oil contains 1, 8-cineole as main ingredient;
4) fully swelling 10g of carbomer in purified water, and uniformly stirring to prepare a carbomer matrix for later use;
5) adding 20g of eucalyptus oil, 100g of glycerol and 20g of polysorbate 80 into the mixed solution I, and uniformly mixing to obtain a mixed solution II;
6) and (3) adding the mixed solution II obtained in the step 5) into a carbomer matrix, uniformly stirring, adding 8g of triethanolamine, adding a proper amount of purified water to enable the total mass to reach 1000g, uniformly stirring, and preparing the gynecological external antibacterial gel.
Example 3
A preparation method of gynecological external antibacterial gel comprises:
1) weighing 20g of herba patriniae, 20g of ephedra herb and 10g of radix clematidis, and preparing herba patriniae distillate by the herba patriniae with a distillation method for later use; then adding herba ephedrae and radix clematidis into the patrinia herb residues obtained after distillation treatment, mixing, adding 8 times of water of the total mass of the medicinal materials, carrying out vacuum decoction for 1.2 hours under reduced pressure, taking decoction, adding 6 times of water of the total mass of the medicinal materials into the residue, carrying out vacuum decoction for 45min under reduced pressure, combining the decoctions, filtering, concentrating to obtain clear paste with the relative density of 1.07, adding 7.5g of sodium benzoate, uniformly mixing, centrifuging, filtering, and preparing a traditional Chinese medicine extract for later use; wherein the herba Patriniae distillate is prepared by drying herba Patriniae in the shade, pulverizing into coarse powder, distilling under normal pressure for 1 hr, collecting primary distillate, and redistilling to obtain herba Patriniae distillate;
2) mixing the traditional Chinese medicine extract obtained in the step 1) and the patrinia distillation liquid to obtain a mixed liquid I;
3) distilling fresh leaves and stems of Eucalyptus globulus Labill to obtain oleum Eucalypti, wherein the essential oil contains 1, 8-cineole as main ingredient;
4) fully swelling 8g of carbomer in purified water, and uniformly stirring to prepare a carbomer matrix for later use;
5) adding 20g of eucalyptus oil, 100g of glycerol and 20g of polysorbate 80 into the mixed solution I, and uniformly mixing to obtain a mixed solution II;
6) and (3) adding the mixed solution II obtained in the step 5) into a carbomer matrix, uniformly stirring, adding 7g of triethanolamine, adding a proper amount of purified water to enable the total mass to reach 1000g, uniformly stirring, and preparing the gynecological external antibacterial gel.
Example 4
A preparation method of gynecological external antibacterial gel comprises:
1) weighing 20g of herba patriniae, 20g of ephedra herb and 10g of radix clematidis, and preparing herba patriniae distillate by the herba patriniae with a distillation method for later use; then adding herba ephedrae and radix clematidis into the patrinia herb residues obtained after distillation treatment, mixing, adding 8 times of water of the total mass of the medicinal materials, carrying out vacuum decoction for 1.8 hours under reduced pressure, taking decoction, adding 6 times of water of the total mass of the medicinal materials into the residue, carrying out vacuum decoction for 30min under reduced pressure, combining the decoctions, filtering, concentrating to obtain clear paste with the relative density of 1.06, adding 7.5g of sodium benzoate, uniformly mixing, centrifuging, filtering, and preparing a traditional Chinese medicine extract for later use; wherein the herba Patriniae distillate is prepared by drying herba Patriniae in the shade, pulverizing into coarse powder, distilling under normal pressure for 1 hr, collecting primary distillate, and redistilling to obtain herba Patriniae distillate;
2) mixing the traditional Chinese medicine extract obtained in the step 1) and the patrinia distillation liquid to obtain a mixed liquid I;
3) distilling fresh leaves and stems of Eucalyptus globulus Labill to obtain oleum Eucalypti, wherein the essential oil contains 1, 8-cineole as main ingredient;
4) swelling 9g of carbomer in purified water, and stirring to obtain carbomer matrix;
5) adding 20g of eucalyptus oil, 95g of glycerol and 20g of polysorbate 80 into the mixed solution I, and uniformly mixing to obtain a mixed solution II;
6) and (3) adding the mixed solution II obtained in the step 5) into carbomer matrix, uniformly stirring, adding 6g of triethanolamine, adding a proper amount of purified water to enable the total mass to reach 1000g, uniformly stirring, and preparing the gynecological external antibacterial gel.
Example 5
A preparation method of gynecological external antibacterial gel comprises:
1) weighing 20g of herba patriniae, 20g of ephedra herb and 10g of radix clematidis, and preparing herba patriniae distillate by the herba patriniae with a distillation method for later use; then adding herba ephedrae and radix clematidis into the patrinia herb residues obtained after distillation treatment, mixing, adding 8 times of water of the total mass of the medicinal materials, carrying out vacuum decoction for 2 hours under reduced pressure, taking decoction, adding 6 times of water of the total mass of the medicinal materials into the residue, carrying out vacuum decoction for 40 minutes under reduced pressure, combining the decoctions, filtering, concentrating to obtain clear paste with the relative density of 1.08, adding 7.5g of sodium benzoate, uniformly mixing, centrifuging, filtering, and preparing a traditional Chinese medicine extract for later use; wherein the herba Patriniae distillate is prepared by drying herba Patriniae in the shade, pulverizing into coarse powder, distilling under normal pressure for 1.7 hr, collecting primary distillate, and redistilling to obtain herba Patriniae distillate;
2) mixing the traditional Chinese medicine extract obtained in the step 1) and the patrinia distillation liquid to obtain a mixed liquid I;
3) distilling fresh leaves and stems of Eucalyptus globulus Labill to obtain oleum Eucalypti, wherein the essential oil contains 1, 8-cineole as main ingredient;
4) fully swelling 10g of carbomer in purified water, and uniformly stirring to prepare a carbomer matrix for later use;
5) adding 20g of eucalyptus oil, 85g of glycerol and 20g of polysorbate 80 into the mixed solution I, and uniformly mixing to obtain a mixed solution II;
6) and (3) adding the mixed solution II obtained in the step 5) into a carbomer matrix, uniformly stirring, adding 8.5g of triethanolamine, adding a proper amount of purified water to enable the total mass to reach 1000g, uniformly stirring, and preparing the gynecological external antibacterial gel.
Example 6
A preparation method of gynecological external antibacterial gel comprises:
1) weighing 20g of herba patriniae, 20g of ephedra herb and 10g of radix clematidis, and preparing herba patriniae distillate by the herba patriniae with a distillation method for later use; then adding herba ephedrae and radix clematidis into the patrinia herb residues obtained after distillation treatment, mixing, adding 8 times of water of the total mass of the medicinal materials, carrying out vacuum decoction for 1.5 hours under reduced pressure, taking decoction, adding 6 times of water of the total mass of the medicinal materials into the residue, carrying out vacuum decoction for 50min under reduced pressure, combining the decoctions, filtering, concentrating to obtain clear paste with the relative density of 1.09, adding 7.5g of sodium benzoate, uniformly mixing, centrifuging, filtering, and preparing a traditional Chinese medicine extract for later use; wherein the herba Patriniae distillate is prepared by drying herba Patriniae in the shade, pulverizing into coarse powder, distilling under normal pressure for 1.2 hr, collecting primary distillate, and redistilling to obtain herba Patriniae distillate;
2) mixing the traditional Chinese medicine extract obtained in the step 1) and the patrinia distillation liquid to obtain a mixed liquid I;
3) distilling fresh leaves and stems of Eucalyptus globulus Labill to obtain oleum Eucalypti, wherein the essential oil contains 1, 8-cineole as main ingredient;
4) fully swelling 10g of carbomer in purified water, and uniformly stirring to prepare a carbomer matrix for later use;
5) adding 20g of eucalyptus oil, 100g of glycerol and 20g of polysorbate 80 into the mixed solution I, and uniformly mixing to obtain a mixed solution II;
6) and (3) adding the mixed solution II obtained in the step 5) into carbomer matrix, uniformly stirring, adding 6g of triethanolamine, adding a proper amount of purified water to enable the total mass to reach 1000g, uniformly stirring, and preparing the gynecological external antibacterial gel.
The pH value of the antibacterial gel prepared in the embodiments 1-6 of the invention is 5-6, and the antibacterial gel is respectively subjected to bacteriostatic detection, vaginal mucosa irritation detection and skin irritation detection, and the specific detection method comprises the following steps:
bacteriostatic test of Candida albicans
The detection method comprises the following steps: according to a method for detecting the sterilization performance, the antibacterial performance and the stability of a product in appendix C of GB15979-2002 hygienic Standard for Disposable sanitary articles, the antibacterial gel is used for detecting the antibacterial performance of Candida albicans (ATCC 10231 Candida albicans purchased commercially and the strain generation is the fourth generation), the acting time is 2min, 5min, 10min and 20min respectively, the test is repeated for 3 times, and the detection conditions are as follows: the ambient temperature was 20.6 ℃ and the relative humidity was 52%. The results of the bacteriostatic efficiency test are shown in table 1.
TABLE 1
| 2min | 5min | 10min | 20min | |
| Example 1 | 85.6％ | 92.4％ | 98.1％ | 99.9％ |
| Example 2 | 86.2％ | 93.5％ | 99.3％ | 99.9％ |
| Example 3 | 85.9％ | 92.8％ | 98.7％ | 99.9％ |
| Example 4 | 85.7％ | 93.1％ | 98.5％ | 99.9％ |
| Example 5 | 86.4％ | 93.6％ | 99.2％ | 99.9％ |
| Example 6 | 86.3％ | 93.0％ | 98.7％ | 99.9％ |
Second, bacteriostasis test of staphylococcus aureus and escherichia coli
The detection method comprises the following steps: according to a method for detecting the sterilization performance, the antibacterial performance and the stability of a product in appendix C of GB15979-2002 hygienic Standard for Disposable sanitary articles, the antibacterial gel is used for detecting the antibacterial performance of staphylococcus aureus (ATCC 6538 staphylococcus aureus purchased commercially and the strain generation is fourth generation) and escherichia coli (ATCC 8099 escherichia coli purchased commercially and the strain generation is fourth generation), the action time is 2min, 5min, 10min and 20min respectively, the test is repeated for 3 times, and the detection conditions are as follows: the ambient temperature was 20.6 ℃ and the relative humidity was 52%. The results of the bacteriostatic efficiency test are shown in tables 2 and 3.
TABLE 2 bacteriostatic properties of the antimicrobial gel of the present invention against Staphylococcus aureus
| 2min | 5min | 10min | 20min | |
| Example 1 | 83.03 | 85.15％ | 88.48％ | 92.12％ |
| Example 2 | 82.22％ | 85.56％ | 87.78％ | 92.22％ |
| Example 3 | 83.10％ | 84.51％ | 88.31％ | 92.82％ |
| Example 4 | 82.75％ | 85.02％ | 88.21％ | 92.39％ |
| Example 5 | 82.14％ | 84.92％ | 87.88％ | 92.51％ |
| Example 6 | 82.50％ | 85.05％ | 88.16％ | 92.44％ |
TABLE 3 bacteriostatic properties of the antibacterial gel of the present invention against E.coli
| 2min | 5min | 10min | 20min | |
| Example 1 | 99.99％ | >99.99％ | >99.99％ | >99.99％ |
| Example 2 | 99.99％ | >99.99％ | >99.99％ | >99.99％ |
| Example 3 | 99.98％ | >99.99％ | >99.99％ | >99.99％ |
| Example 4 | 99.99％ | >99.99％ | >99.99％ | >99.99％ |
| Example 5 | 99.99％ | >99.99％ | >99.99％ | >99.99％ |
| Example 6 | 99.98％ | >99.99％ | >99.99％ | >99.99％ |
Third, vagina mucosa stimulation experiment
The detection method comprises the following steps: the test is carried out according to the disinfection technical specification (2002 edition) 2.3.5 vagina mucosa stimulation test. New Zealand rabbits (weight 2092.4g-2456.4 g) of common grade, supplied by the Guangdong provincial medical laboratory animal center (Sanshui base), were selected for testing, and had no secretion, congestion, edema and other injury conditions in the vaginal orifice.
The contamination method comprises the following steps: connecting a blunt hose with the length of 8cm with a 2mL syringe, and filling the syringe and the catheter with the antibacterial gel prepared by the invention; fixing the New Zealand rabbits on the back to expose perineum and vaginal opening, wetting the catheter with sodium chloride injection, gently inserting into vagina (4cm-5cm), slowly injecting 2mL of antibacterial gel (that is, the application amount of each New Zealand rabbit is 2mL) with a syringe, and withdrawing the catheter to complete contamination.
24 hours after the infection, the animals are killed by a carbon dioxide method, the whole vagina is taken out after the laparotomy, the longitudinal incision is made, and whether congestion, edema and other manifestations exist is observed by naked eyes.
The test result shows that the irritation of the antibacterial gel to the vaginal mucosa is extremely mild, and the standard of measurement refers to 'disinfection technical specification' (2002 edition) 2.3.13.1 (5).
Four or more complete skin irritation experiments
The detection method comprises the following steps: the test was carried out according to the skin irritation test "Disinfection Specification" (2002 edition) 2.3.3. New Zealand rabbits of the general grade (supplied by the medical laboratory animal center of Guangdong province (Sanshui)) weighing 2011.8g-2227.2g were selected for the test.
In the specific test, the hair at two sides of the spine of the rabbit is removed by using a shaver and depilatory cream 24 hours before the test, the left part and the right part are respectively 3cm by 3cm, and the rabbit is used as a test part and a control part without damaging the animal epidermis. 0.5mL of the antibacterial gel is smeared on a test area for skin contamination, the contamination is carried out for 1 time per day, and the continuous contamination is carried out for 14 days. The control zone was used as a blank control without contamination. Residual test article was removed with water 4h after each exposure, and the results were observed 24h after each exposure and scored for irritation response according to Table 2-11 of Disinfection Specification (2002).
The detection result shows that the antibacterial gel of the invention grades the irritation reaction strength of the complete skin of the New Zealand rabbit for many times into nonirritant, and meets the requirements of 'Disinfection technical Specification' (2002 edition) 2.3.13.1 (3).
The above embodiments are merely examples of the present invention, and it is obvious that the present invention is not limited to the above embodiments, but may be modified. All modifications directly or indirectly derivable by a person skilled in the art from the present disclosure are to be considered within the scope of the present invention.
Claims (7)
1. The gynecological external antibacterial gel is characterized by comprising the following components:
herba Patriniae, herba Ephedrae, radix Clematidis, oleum Eucalypti, sodium benzoate, glycerol, carbomer, polysorbate 80, and triethanolamine; the mass ratio of the patrinia, the ephedra and the clematis root is 2:2: 1.
2. The gynecological external antibacterial gel according to claim 1, wherein the eucalyptus oil is obtained by distilling and extracting fresh leaves and stems of Eucalyptus lobutana, and the main component of the eucalyptus oil is 1, 8-cineole.
3. The gynecological external antibacterial gel according to claim 2, which comprises the following components by weight:
patrinia 20g
Ephedra, herba Ephedrae 20g
Clematis root 10g
Eucalyptus oil 20g
Sodium benzoate 7.5g
80-100g of glycerol
Carbomer 8-10g
Polysorbate 8020g
3-10g of triethanolamine.
4. A method for preparing a gynecological topical antibacterial gel according to any one of claims 1 to 3, characterized by comprising the steps of:
1) weighing herba Patriniae, herba Ephedrae and radix Clematidis at a certain proportion, and distilling herba Patriniae to obtain herba Patriniae distillate; then adding herba Ephedrae and radix Clematidis into the residue of herba Patriniae herb after distillation, decocting with water, concentrating to obtain fluid extract of 1.05-1.10, adding sodium benzoate, mixing, centrifuging, and filtering to obtain Chinese medicinal extract;
2) mixing the traditional Chinese medicine extract obtained in the step 1) and the patrinia distillation liquid to obtain a mixed liquid I;
3) distilling fresh leaves of Eucalyptus parviflora to obtain oleum Eucalypti;
4) fully swelling carbomer in purified water, and stirring to obtain carbomer matrix for later use;
5) adding eucalyptus oil, glycerin and polysorbate 80 into the mixed solution I in proportion, and uniformly mixing to obtain a mixed solution II;
6) and (3) adding the mixed solution II obtained in the step 5) into a carbomer matrix, uniformly stirring, adjusting the pH to 5-6 by using triethanolamine, adding a proper amount of purified water to enable the total mass to reach the specified prescription amount, uniformly stirring, and preparing the gynecological external antibacterial gel.
5. The method of claim 4, wherein the herba Patriniae distillate of step 1) is prepared by drying herba Patriniae in the shade, pulverizing into coarse powder, distilling under normal pressure for 1-2 hr to obtain primary distillate, and redistilling the primary distillate to obtain herba Patriniae distillate.
6. The preparation method according to claim 5, wherein the traditional Chinese medicine extract in the step 1) is prepared by mixing herba patriniae dregs, ephedra herb and radix clematidis in proportion, adding 8 times of water of the total mass of the medicinal materials, carrying out vacuum decoction for 1-2 hours under reduced pressure, taking decoction, adding 6 times of water of the total mass of the medicinal materials into the dregs, carrying out vacuum decoction for 30-60 min under reduced pressure, combining the decoction, filtering, and concentrating to obtain the clear paste with the relative density of 1.05-1.10.
7. The preparation method of claim 5, wherein the eucalyptus oil in step 3) is obtained by pulverizing fresh leaves and stems of Eucalyptus parviflora into coarse powder, and distilling under normal pressure to extract essential oil.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1724012A (en) * | 2005-07-15 | 2006-01-25 | 刘振松 | Traditional Chinese medicine contg. three kinds of herb medicines, and its prepn. method |
| CN109820901A (en) * | 2019-04-19 | 2019-05-31 | 山东步长神州制药有限公司 | A kind of Traditional Chinese medicinal gel preparation with sterilization |
| CN111803567A (en) * | 2020-06-15 | 2020-10-23 | 广西中医药大学 | Camellia oil antibacterial and anti-inflammatory gel and preparation method thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1724012A (en) * | 2005-07-15 | 2006-01-25 | 刘振松 | Traditional Chinese medicine contg. three kinds of herb medicines, and its prepn. method |
| CN109820901A (en) * | 2019-04-19 | 2019-05-31 | 山东步长神州制药有限公司 | A kind of Traditional Chinese medicinal gel preparation with sterilization |
| CN111803567A (en) * | 2020-06-15 | 2020-10-23 | 广西中医药大学 | Camellia oil antibacterial and anti-inflammatory gel and preparation method thereof |
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