CN113519559A - Biocontrol fungus field propagation agent - Google Patents
Biocontrol fungus field propagation agent Download PDFInfo
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- CN113519559A CN113519559A CN202110823033.6A CN202110823033A CN113519559A CN 113519559 A CN113519559 A CN 113519559A CN 202110823033 A CN202110823033 A CN 202110823033A CN 113519559 A CN113519559 A CN 113519559A
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- Prior art keywords
- spore
- biocontrol
- agent
- biocontrol fungus
- nutrient medium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
Abstract
The invention discloses a biocontrol fungus field propagation agent, which comprises the following components in percentage by weight, when a cultured solid biocontrol fungus spore agent is dried and crushed into spore powder, the spore powder accounts for 20-80%, and the balance is dried and crushed nutrient medium. The invention is composed of biocontrol fungus spore and nutrient medium, the propagation agent is applied in the field, the biocontrol fungus grows and propagates in the field, the spore amount is enlarged, the purpose of comprehensive protection is achieved, the protective effect is achieved, the biocontrol fungus releases a large amount of volatile antibiotics in the field growing and propagating process, the germination of the fungal spore with diseases on the soil surface and the crop stem, leaf, flower and fruit part and the growth of hypha can be inhibited, the disease prevention effect is achieved, the improved new technical scheme reduces the proportion of the spore agent, reduces the cost, increases the proportion of the spore agent, and is suitable for crops with larger economic value or users with longer storage time and smaller consumption.
Description
Technical Field
The invention relates to the field of microorganism application, in particular to a biocontrol fungus field propagation agent.
Background
Researches prove that the biocontrol fungi can effectively antagonize a plurality of plant disease fungi such as fusarium, sclerotium rolfsii, rhizoctonia, phytophthora, rhizoctonia solani, botrytis cinerea, wheat glume blight and the like, so that the aim of preventing plant diseases is fulfilled. The biological control fungus is applied in the field, has no worry of chemical pesticide residue, and is particularly suitable for the field of pollution-free agriculture.
After the antagonism mechanism of the self-biological control fungi is widely agreed, experts and scholars at home and abroad can not be used in agriculture at present, but the expert and scholars can not be used for agriculture. The reason is that the method has five aspects:
1. the form of the preparation, most of the products are spore powder preparations, and the application method mainly comprises soil mixing and broadcasting, direct mixing into a plough layer, a planting hole and spraying. The application methods of soil mixing and spreading, direct mixing into a plough layer and planting holes are influenced by the antibacterial factors in the soil, the spraying is influenced by the climate and the environment, and the spore germination, hypha growth and colonization of the biocontrol fungi are not ideal. The spore survival period of the fungus is short, partial inactivation exists in the transportation and storage of commercial preparations, and the application method influences the spore germination, hypha growth and colonization after application under the condition that the quantity of the viable spores is reduced;
2. The concept is not clear, the bio-control fungi are mostly regarded as an organism for preventing and treating plant diseases in the industry at present, and the bio-control fungi only can prevent the plant diseases and cannot cure the diseased plants;
3. the application method is complicated, and the practical application of agriculture is difficult;
4. the cost is high;
5. the effect is not obvious.
Disclosure of Invention
The technical problem to be solved by the invention is to overcome the defects of the prior art and provide a biocontrol fungus field propagation agent.
In order to solve the technical problems, the invention provides the following technical scheme:
the invention relates to a field propagation agent for biocontrol fungi, which comprises the following components in percentage by weight:
a. when the cultured solid biocontrol fungus spore agent is dried and crushed into spore powder, the weight percentage of the spore powder is 20-80 percent, and the rest is dried and crushed nutrient medium;
b. when the liquid biocontrol fungus spore agent obtained by culturing is spore liquid, the weight percentage of the spore liquid is 25-85 percent, and the rest is dried and crushed nutrient medium;
c. when the spore powder and the spore liquid are mixed into the biocontrol fungal spore agent, the weight percentage of the spore powder and the spore liquid is 30-75 percent, and the balance is dried and crushed nutrient medium.
In a preferred embodiment of the present invention, the biocontrol fungi is one or a combination of two or a combination of several of the biocontrol fungi in fungi kingdom.
In a preferred embodiment of the present invention, the biocontrol fungal spore is one or a combination of two or a combination of several fungal spore species.
In a preferred embodiment of the present invention, the biocontrol fungus spore is obtained by culturing the biocontrol fungus with the following materials as culture media:
A. grains, seeds, or other by-products;
B. stems, leaves, roots, flowers, fruits of plants or mixtures thereof;
C. animal skins, hair, meat or mixtures thereof;
A. b, C combinations of two of the three or more.
As a preferable technical scheme of the invention, minerals are added into the nutrient medium and the culture medium.
As a preferred technical scheme of the invention, the nutrient medium is as follows:
A. grains, seeds, or other by-products;
B. stems, leaves, roots, flowers, fruits of plants or mixtures thereof;
C. animal skins, hair, meat or mixtures thereof;
A. b, C combinations of two of the three or more.
As a preferable technical scheme of the invention, the spore liquid is filtered before being mixed with the nutrient medium.
In a preferred embodiment of the present invention, the drying and pulverizing are performed by a drying and pulverizing machine.
Compared with the prior art, the invention has the following beneficial effects:
the invention consists of biocontrol fungus spores and nutrient medium, the propagation agent is applied in the field, the biocontrol fungus grows and propagates in the field, the spore amount is enlarged, the purpose of comprehensive protection is achieved, the enlarged spores can be released and scattered, part of the spores can land on the parts of stems, leaves, flowers and fruits of crops which are possibly damaged by disease fungi to germinate, grow and colonize, the biocontrol fungus can play a role in protection, a large amount of volatile antibiotics can be released in the growth and propagation processes of the field, the method can inhibit germination of fungal spores and growth of hyphae on soil surfaces and crop stems, leaves, flowers and fruits to achieve the effect of disease prevention, and the improved new technical scheme reduces the proportion of spore agents, reduces the cost, increases the proportion of the spore agents, is suitable for crops with higher economic value or users with longer storage time and smaller dosage, and enhances the disease prevention effect.
Detailed Description
The following description of the preferred embodiments of the present invention is provided for the purpose of illustration and description, and is in no way intended to limit the invention.
Example 1
The invention provides a biocontrol fungus field propagation agent, which comprises the following components in percentage by weight:
a. when the cultured solid biocontrol fungus spore agent is dried and crushed into spore powder, the weight percentage of the spore powder is 20-80 percent, and the rest is dried and crushed nutrient medium;
b. when the liquid biocontrol fungus spore agent obtained by culturing is spore liquid, the weight percentage of the spore liquid is 25-85 percent, and the rest is dried and crushed nutrient medium;
c. when the spore powder and the spore liquid are mixed into the biocontrol fungal spore agent, the weight percentage of the spore powder and the spore liquid is 30-75 percent, and the balance is dried and crushed nutrient medium.
The biocontrol fungus field propagation agent is used for preventing gray mold of vegetables and strawberries:
the formula of the spore powder culture medium of the trichoderma fungus trichoderma harzianum two strains used is 500 g of wheat bran, 360 g of rapeseed cakes, 18 g of meat and bone meal, 1.2 g of monopotassium phosphate, 0.8 g of potassium nitrate and 0.8 g of sodium tetraborate; the formula of the nutrient medium comprises 500 g of wheat bran, 360 g of rapeseed cakes, 18 g of meat and bone meal, 1.2 g of monopotassium phosphate, 0.8 g of potassium nitrate and 0.8 g of sodium tetraborate, the granularity of the nutrient medium is below 4mm, the weight ratio of spore powder to the nutrient medium is 10:66, the first application amount of the nutrient medium is 110 kg per hectare before the crop is attacked in the attack season, the second application amount of the nutrient medium is 40 kg per hectare after 1 month interval, the application is carried out twice in the embodiment, and the disease prevention effect reaches 92%.
Example 2
Biocontrol fungus field propagation agent for preventing cotton rhizoctonia disease
The strain used in the embodiment is a strain of trichoderma harzianum which is a trichoderma fungus, and the formula of the spore powder culture medium comprises 600 g of wheat bran, 340 g of rapeseed cakes, 80 g of corn flour, 16 g of meat and bone meal, 0.8 g of monopotassium phosphate, 0.8 g of potassium nitrate and 0.8 g of sodium tetraborate; the formula of the nutrient medium comprises 600 g of wheat bran, 340 g of rapeseed cake, 90 g of rice meal, 16 g of meat and bone meal, 0.8 g of monopotassium phosphate, 0.8 g of potassium nitrate and 0.8 g of sodium tetraborate, the granularity of the nutrient medium is less than 2.8mm, and the weight ratio of spore powder to the nutrient medium is 20: 47, 48 kg is applied to each hectare before seeding, 32 kg is applied to each hectare at intervals of 2 months, the application is carried out twice in the embodiment, and the disease prevention effect reaches 94 percent.
Example 3
Biocontrol fungus field propagation agent for preventing sclerotinia rot of colza
The strain used in the embodiment is a broomcorn broomrape fungus strain, the formula of a spore powder culture medium is 450 g of wheat bran, 320 g of rapeseed cakes, 80 g of rice flour, 20 g of meat and bone meal, 0.6 g of monopotassium phosphate, 0.6 g of potassium nitrate and 0.6 g of sodium tetraborate, the formula of a nutrient medium is 450 g of wheat bran, 320 g of rapeseed cakes, 20 g of meat and bone meal, 0.6 g of monopotassium phosphate, 0.6 g of potassium nitrate and 0.6 g of sodium tetraborate, the granularity of the nutrient medium is below 2.5mm, the weight ratio of the spore powder to the nutrient medium is 1:19, the strain is applied before crop diseases occur in a disease season, the dosage is 50 kg per hectare at the first time, the application is performed once every 1 month later, the dosage is 20 kg per hectare, the application is performed twice in the embodiment, and the disease prevention effect is 88%.
Example 4
Biocontrol fungus field propagation agent for preventing sweet pepper and phytophthora capsici
The strain used in the embodiment is Trichoderma viride strain of Trichoderma, the formula of the spore powder culture medium is 460 g of wheat bran, 340 g of rapeseed cake, 80 g of mixed tree leaf powder, 1.0 g of monopotassium phosphate, 1.0 g of potassium nitrate and 1.0 g of sodium tetraborate, the formula of the nutrient medium is 460 g of wheat bran, 340 g of rapeseed cake, 80 g of mixed tree leaf powder, 1.0 g of monopotassium phosphate, 1.0 g of potassium nitrate and 1.0 g of sodium tetraborate, the granularity of the nutrient medium is below 1.8mm, the weight ratio of the spore powder to the nutrient medium is 20:30, the strain is applied after seeding, 40 kg of strain is applied per hectare, 25 kg of strain per hectare applied at intervals of 2 months, the embodiment is applied twice, and the disease prevention effect reaches 91%.
Example 5
Biocontrol fungus field propagation agent for preventing eggplant damping off and southern blight
The strain used in the embodiment is a coniothyrium fungus strain, the formula of the spore powder culture medium is 500 g of wheat bran, 360 g of rapeseed cake, 90 g of corn stalk powder, 20 g of meat and bone meal, 1.2 g of monopotassium phosphate, 1.2 g of potassium nitrate and 1.2 g of sodium tetraborate, the formula of the nutrient medium is 500 g of wheat bran, 360 g of rapeseed cake, 90 g of corn stalk powder, 20 g of meat and bone meal, 1.2 g of monopotassium phosphate, 1.2 g of potassium nitrate and 1.2 g of sodium tetraborate, and the granularity of the nutrient medium is below 2.5 mm.
Example 6
Biocontrol fungus field propagation agent for preventing tomato wilt
The strain used in the embodiment is a chaetomium fungus strain, the formula of a spore powder culture medium is 600 g of wheat bran, 4800 g of rapeseed cake, 150 g of corn flour, 22 g of meat and bone meal, 1.3 g of monopotassium phosphate, 1.3 g of potassium nitrate and 1.3 g of sodium tetraborate, the formula of a nutrient medium is 600 g of wheat bran, 4800 g of rapeseed cake, 150 g of corn flour, 22 g of meat and bone meal, 1.3 g of monopotassium phosphate, 1.3 g of potassium nitrate and 1.3 g of sodium tetraborate, the granularity of the nutrient medium is below 3.2mm, and the weight ratio of spore powder to the nutrient medium is 7: 13, the application is carried out according to the method before the crops are attacked in the attack season, the dosage per hectare is 50 kg for the first time, the dosage per hectare is 25 kg for the next time at intervals of 2 months, the application is carried out for 2 times in the embodiment, and the disease prevention effect reaches 87 percent.
In the above examples, the spore powder and the spore liquid can achieve better disease prevention effect, and the biocontrol fungal spore agent has better disease prevention effect when the weight percentage of the biocontrol fungal spore agent is 45-65%.
The invention consists of biocontrol fungus spores and nutrient medium, the propagation agent is applied in the field, the biocontrol fungus grows and propagates in the field, the spore amount is enlarged, the purpose of comprehensive protection is achieved, the enlarged spores can be released and scattered, part of the spores can land on the parts of stems, leaves, flowers and fruits of crops which are possibly damaged by disease fungi to germinate, grow and colonize, the biocontrol fungus can play a role in protection, a large amount of volatile antibiotics can be released in the growth and propagation processes of the field, the method can inhibit germination of fungal spores and growth of hyphae on soil surfaces and crop stems, leaves, flowers and fruits to achieve the effect of disease prevention, and the improved new technical scheme reduces the proportion of spore agents, reduces the cost, increases the proportion of the spore agents, is suitable for crops with higher economic value or users with longer storage time and smaller dosage, and enhances the disease prevention effect.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. The biocontrol fungus field propagation agent is characterized by comprising the following components in parts by weight:
a. when the cultured solid biocontrol fungus spore agent is dried and crushed into spore powder, the weight percentage of the spore powder is 20-80 percent, and the rest is dried and crushed nutrient medium;
b. when the liquid biocontrol fungus spore agent obtained by culturing is spore liquid, the weight percentage of the spore liquid is 25-85 percent, and the rest is dried and crushed nutrient medium;
c. when the spore powder and the spore liquid are mixed into the biocontrol fungal spore agent, the weight percentage of the spore powder and the spore liquid is 30-75 percent, and the balance is dried and crushed nutrient medium.
2. The biocontrol fungus field propagation agent as claimed in claim 1, wherein the biocontrol fungus is one or a combination of two or a combination of several of the biocontrol fungi in the fungal kingdom.
3. The biocontrol fungus field propagation agent as claimed in claim 1, wherein the biocontrol fungus spores are one or a combination of two or a combination of several of the fungal spore species.
4. The biocontrol fungus field propagation agent as claimed in claim 1, wherein the biocontrol fungus spores are obtained by culturing biocontrol fungus with the following materials as culture media:
A. grains, seeds, or other by-products;
B. stems, leaves, roots, flowers, fruits of plants or mixtures thereof;
C. animal skins, hair, meat or mixtures thereof;
A. b, C combinations of two of the three or more.
5. The biocontrol fungus field propagation agent as claimed in claims 1 and 4, wherein minerals are added to both the nutrient medium and the culture medium.
6. The biocontrol fungus field propagation agent as claimed in claim 1, wherein the nutrient medium is:
A. grains, seeds, or other by-products;
B. Stems, leaves, roots, flowers, fruits of plants or mixtures thereof;
C. animal skins, hair, meat or mixtures thereof;
A. b, C combinations of two of the three or more.
7. The biocontrol fungus field propagation agent as claimed in claim 1, wherein moisture is filtered out before the spore solution is mixed with the nutrient medium.
8. The biocontrol fungus field propagation agent as claimed in claim 1, wherein the drying and crushing are performed by a drying and crushing all-in-one machine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110823033.6A CN113519559A (en) | 2021-07-21 | 2021-07-21 | Biocontrol fungus field propagation agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110823033.6A CN113519559A (en) | 2021-07-21 | 2021-07-21 | Biocontrol fungus field propagation agent |
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| Publication Number | Publication Date |
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| CN113519559A true CN113519559A (en) | 2021-10-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110823033.6A Withdrawn CN113519559A (en) | 2021-07-21 | 2021-07-21 | Biocontrol fungus field propagation agent |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003065812A1 (en) * | 2002-02-05 | 2003-08-14 | Council Of Scientific And Industrial Research | Novel composition for early and profuse sporulation in fungi and a method thereof |
| CN101473855A (en) * | 2009-02-16 | 2009-07-08 | 何松庆 | Propagating agent for preventing fungus in field |
-
2021
- 2021-07-21 CN CN202110823033.6A patent/CN113519559A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003065812A1 (en) * | 2002-02-05 | 2003-08-14 | Council Of Scientific And Industrial Research | Novel composition for early and profuse sporulation in fungi and a method thereof |
| CN101473855A (en) * | 2009-02-16 | 2009-07-08 | 何松庆 | Propagating agent for preventing fungus in field |
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Application publication date: 20211022 |