CN113519524A - Plant immunomodulator and application thereof - Google Patents
Plant immunomodulator and application thereof Download PDFInfo
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- CN113519524A CN113519524A CN202010317764.9A CN202010317764A CN113519524A CN 113519524 A CN113519524 A CN 113519524A CN 202010317764 A CN202010317764 A CN 202010317764A CN 113519524 A CN113519524 A CN 113519524A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/08—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
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Abstract
The invention discloses a plant immunomodulator and application thereof, wherein the plant immunomodulator comprises the following components in parts by weight: 5-40 parts of L-ascorbic acid and 60-95 parts of filler, wherein the best composition is an ascorbic acid water agent with the concentration of 8.5%. Can be effectively applied to preventing and controlling virus diseases of green plants, namely preventing and controlling the occurrence, the spread and the harm of diseases such as 'potato virus Y, tobacco virus Y, bacterial wilt, black shank' and the like. Fully exerts the antioxidation effect of the active substances, participates in the oxidation-reduction reaction of organisms, promotes the generation of interferon, interferes the transcription of plant virus mRNA, inhibits the virus proliferation, inhibits the negative immune regulation, prevents the variation of cells and ensures the integrity of the cells of the organisms; promoting catalytic metabolism, enhancing immune system, enhancing organism resistance, resisting oxidation, resisting aging, and improving disease-resistant immunity; promoting the growth, development and maturation of organisms. Has the special effects of resisting drought, disease and cold, promoting the growth, development and maturity of crops, increasing the yield, improving the quality of the crops and the like.
Description
Technical Field
The invention belongs to the technical field of green plant prevention and control preparations, and particularly relates to a plant immunomodulator, a preparation method and application thereof.
Background
For a long time, when people prevent and control harmful target organisms such as diseases, insects, grasses, rats and the like, corresponding medicaments are mostly selected according to the types of the target organisms for prevention and control. Therefore, some high-efficiency, high-toxicity and high-residue pesticide varieties must be applied for a long time or even abused, so that the ecological environment is polluted, target organisms are polluted, drug resistance is easy to generate, normal metabolism of host crops is hindered, enzyme activity is inhibited, and normal physiological and biochemical processes cannot be finished. Poor adverse performance and loss of immunity, which inevitably leads to rampant and concentrated harm of certain target organisms, causes local or large-area damage of crops, greatly reduces the yield, reduces the quality and exceeds the standard of pesticide residue of agricultural products.
At present, the regulator researched at home and abroad mostly focuses on the functions of crop growth and yield increase, the field application effect is not ideal, and the re-application effect is not ideal when harmful target organisms occur, spread and harm. The innovative development of the green prevention and control preparation immunomodulator which not only has the immune induction mechanism, regulates the physiological function, promotes the growth, development and maturity of crops, but also can effectively reduce, prevent and control the occurrence, the spread and the harm of harmful target organisms of green plants is urgently needed, and the problem of continuously developing organic agriculture high-quality production in China is solved.
Based on the above starting points, according to the physiological characteristics of high-energy trace organic nutrient elements required for the growth and development of organisms for many years; the occurrence, spreading and damage rule of pest and disease damage of harmful target organisms; and many research results accumulated empirically over many years. Firstly, taking high-energy trace organic nutrient element L ascorbic acid produced by microbial secondary fermentation as a raw medicine, carrying out formula design, and integrally and innovatively developing a green prevention and control preparation with independent intellectual property rights, namely an immunomodulator-8.5% ascorbic acid water agent, through repeated screening of indoor bioassay, field test and production demonstration. In addition. The product with the same proportion of the ascorbic acid water agent of 8.5 percent is not reported at home and abroad, is initiated at home, and has the special effects of resisting drought, diseases and cold, promoting the growth and the development of crops, improving the yield, improving the quality of the crops and the like.
Disclosure of Invention
The invention provides a plant immunomodulator, a preparation method and application thereof, aiming at solving the technical problems of generation, spread and harm rules of harmful target organisms and the physiological characteristics of high-energy trace organic nutrient elements required by the growth and development of organisms.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a plant immunomodulator comprises the following components in parts by weight:
5-40 parts of L-ascorbic acid and 60-95 parts of a filler.
Preferably, the plant immunomodulator consists of the following components in parts by weight:
7-10 parts of L-ascorbic acid and 90-93 parts of a filler.
Preferably, the plant immunomodulator consists of the following components in parts by weight:
8.5 parts of L-ascorbic acid and 91.5 parts of filler.
The plant immunomodulator has the following applications (A) and (B) in the growth of green plants:
(A) increasing the yield;
(B) preventing and controlling virus diseases of green plants.
The method for preventing and controlling the virus diseases of the green plants specifically comprises the following applications:
(b1) preventing and alleviating potyvirus;
(b2) preventing and alleviating tobacco potato virus Y;
(b3) preventing and relieving black shank;
(b4) preventing and relieving bacterial wilt.
Further, the method is particularly applied to:
1) grain crops including potatoes, rice, upland rice, wheat, corn;
2) commercial crops including tobacco, tea, peanut, rape;
3) vegetables including Chinese cabbage, caulis et folium Brassicae Capitatae, herba Apii Graveolentis, herba Alii Fistulosi, fructus Solani Melongenae, and Capsici fructus;
4) melons, including watermelon and cucumber;
5) fruits including strawberry, grape, peach, plum, apple, and orange;
6) green Chinese medicinal materials including radix Notoginseng, rhizoma Polygonati, radix Ginseng, radix Panacis Quinquefolii, and rhizoma Polygoni Multiflori.
Has the advantages that: the plant immunomodulator of the invention fully exerts the antioxidation effect of the active component, reduces the disulfide bond (-S-S) to-SH, captures free radicals, and eliminates H2O2And O2Resisting free radical damage to body cells, enhancing intercellular permeability, maintaining cell integrity and metabolism, promoting interferon production, interfering plant virus mRNA transcription, inhibiting virus proliferation, inhibiting negative immune regulation, strengthening immune system, and enhancing immunityThe organism has the advantages of stress resistance, oxidation resistance and aging resistance, the disease-resistant immunity is improved, the occurrence, the spread and the harm of virus diseases such as potato virus Y, tobacco virus Y, black shank, bacterial wilt and the like of green plants are effectively reduced and prevented, and the disease index is obviously reduced. The plant growth regulator has the special effects of resisting drought, diseases and cold, promoting the growth, development and maturity of crops, increasing the yield, improving the quality of the crops and the like.
Detailed Description
FIG. 1 is a flow chart of the optimization process and safety evaluation of the components of the present invention.
Detailed Description
The invention is illustrated below with reference to specific examples. It will be understood by those skilled in the art that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention in any way.
A plant immunomodulator comprises the following components in parts by weight: 5-40 parts of L-ascorbic acid and 60-95 parts of a filler. As shown in fig. 1, the optimal assembly is obtained by optimizing the process flow and the safety evaluation process; the specific process comprises the following steps:
(1) selecting L ascorbic acid technical product produced by microorganism secondary fermentation technology, repeatedly screening formula design, and determining optimal formulation;
(2) through a small-area test, a demonstration test and a yield increase effect, the process flow and safety evaluation are optimized, and the optimal composition is a green prevention and control preparation of an immunomodulator-8.5% ascorbic acid water agent, so that the virus diseases, bacterial wilt and black shank of green plants are effectively reduced and prevented and controlled, the using amount of chemical pesticides and chemical fertilizers is reduced, and the quality is improved while the yield is increased.
Example 1: effect of increasing yield of potato and tobacco
1) The plant immune regulator comprises the following components in percentage by weight: 8.5 parts of L-ascorbic acid and 91.5 parts of a filler;
2) the application method comprises the following steps: 1500-2000 times of liquid spraying;
3) the action mechanism is as follows: the potato virus Y is recorded as early as 30 s, China is the Guizhou of potato kingdom and the province of potato, the habit of continuous cropping of potato and tobacco exists in ancient times, and the potato virus Y must be recordedBut also becomes an infection source for spreading and damaging tobacco, and the tobacco is gradually and violently spread and damaged, and the production momentum is lacking in effective medicament prevention and control. The green prevention and control of tobacco potato Y virus must adopt 'immunomodulator-8.5% ascorbic acid water agent' 1500 culture solution through aerosol method in artificial climate chamber-600 m21.07 ten thousand potato seedlings can be planted at one time to ensure that roots grow more, 1500 seeds are added for collecting 100 single plants in a treatment area compared with a contrast area on average, 1070 seeds and 1605 ten thousand potato detoxified seeds accounting for 20 percent of the planned number are added in total, the produced potato-free Y virus seeds are applied to the actual production, the great problem that the tobacco is harmed by the spread of the potato Y virus in a continuous cropping area of the potatoes and the tobacco is solved, namely, the damage of the potato Y virus is effectively reduced and prevented and controlled, and the yield is increased and the potato degeneration problem of the potato seeds is solved, so that the great problem of the high-quality production of green tobacco is solved;
4) the prevention and control process comprises the following steps: the tobacco leaf production department of Guizhou tobacco company cooperates with Bijie company, Guiyang city company and Qiandongnan province company to implement a 'green prevention and control project', from 2015 to 2019, in the production process, a new mode for producing a virus-free robust tobacco seedling green prevention and control preparation is established, wherein an 'immune inducer-8.5% ascorbic acid water agent' is accurately sprayed in a tobacco seedbed stage to enhance the stress resistance, oxidation resistance and ageing resistance of an organism and improve the disease-resistant immunity of the organism, a new mode for producing the virus-free robust tobacco seedling green prevention and control preparation is established, the emergence rate is up to more than 98%, 1500 culture solutions are respectively irrigated once along with fixed root water and water-based fertilizer in a group period when the virus-free robust tobacco seedlings produced by transplanting medium seedlings, namely 100 and 150ml of a single plant, the rapid growth and development of tobacco plants are promoted, the maturity is particularly promoted, the top leaves are cut and baked once, the economic benefit of the upper tobacco leaves is improved, and the Y virus of tobacco potatoes can be effectively reduced, prevented and controlled, The disease such as tobacco black shin and bacterial wilt and the like is damaged, the problem of high-quality production is solved, the tobacco can recover the economic loss by 400-600 yuan per mu, 0.6-0.9-trillion yuan per hectare and 0.36-0.54-trillion yuan per 6000 hectare; the national tax-increasing income, the agricultural income-increasing income and the peasant synergistic benefit are obvious and are deeply welcomed by tobacco growers.
Example 2: effect on increasing yield of rice
1) The plant immune regulator comprises the following components in percentage by weight: 8.5 parts of L-ascorbic acid and 91.5 parts of a filler;
2) the application method comprises the following steps: 1500-2000 times of liquid spraying;
3) a place: 2015 + 2019, respectively implementing ten thousand mu test demonstration points in the Kaiyang county of Guiyang city in Guizhou province and the Tanzhou district in Zuyi city;
4) the effect is as follows: 1-2 true leaves, the tillering stage, the booting stage or the heading stage of the rice seedling field are respectively and accurately sprayed with 'an immunomodulator-8.5 percent ascorbic acid aqua' 1500-2000 times liquid, 600 kilograms per hectare, so that the effective tillering rate, the heading rate and the thousand seed weight of the rice are improved, and the yield is increased by 15-20 percent. 667 hectare production demonstration, on average each hectare net increase rice 1623.3kg, total net increase rice more than 108.3 kilo, agricultural income increase, peasant increase efficiency, ecological benefit, social benefit and economic benefits are obvious.
Example 3: yield increasing effect on Chinese cabbage
1) The plant immune regulator comprises the following components in percentage by weight: 8.5 parts of L-ascorbic acid and 91.5 parts of a filler;
2) the application method comprises the following steps: 1500-2000 times of liquid spraying;
3) a place: 2015 + 2019, 500 hectares of Kaiyang county of Guiyang city of Guizhou province and Kaili city of Qian southeast China are respectively used for production demonstration tests;
4) the effect is as follows: the 'immune attractant-8.5 percent ascorbic acid water agent' 1500-fold 2000-fold liquid is accurately sprayed in three growth periods of seedling period, tray pulling period and heading period of the Chinese cabbage, 40 kilograms of the liquid is sprayed per mu, the yield can be increased by 15 to 30 percent, and the average prevention effect of virus diseases and downy mildew of the Chinese cabbage can be effectively reduced and controlled by over 90 percent. The average yield per mu of the treatment area is 4750 kilograms, the average yield per mu of the control area is 3615 kilograms, the yield is increased by 23.89 percent, the average yield per mu of the treatment area is 0.1 ten thousand kilograms more than that of the control area, 0.4 ten thousand yuan more is required to be added by calculating 4 yuan per kilogram, and 6 ten thousand yuan is required to be added per hectare on average; the yield of 500 hectares is 0.3 hundred million yuan, the agricultural production increase, the vegetable grower income increase, the ecological benefit, the social benefit and the economic benefit are obvious, and the input-output ratio is 1: 10-15.
Example 4: yield increasing effect on strawberries
1) The plant immune regulator comprises the following components in percentage by weight: 8.5 parts of L-ascorbic acid and 91.5 parts of a filler;
2) the application method comprises the following steps: 1500-2000 times of liquid spraying;
3) a place: accurately spraying a green prevention and control preparation, namely an immunomodulator-8.5 percent ascorbic acid water agent, 1500-fold and 2000-fold liquid to strawberries in a Huaxi district of Guiyang city;
4) the effect is as follows: 2015 + 2019 strawberries are accurately sprayed with a green prevention and control preparation, namely an immunomodulator-8.5% ascorbic acid water agent 1500 culture solution and grapes and other fruits at the bud stage, the young fruit stage and the fruit swelling stage, the strawberries are put into the market in advance for 7-10 days in the Huaxi area of Guiyang city for many years, the harvesting period is prolonged for 10-15 days, namely one fruit is added, the dosage of chemical pesticides and fertilizers is reduced, the crop quality is improved by increasing 20%, the content of soluble solids is improved by 15%, the yield value increased by 450 + 600 yuan per mu is calculated, the yield value increased by 450 + 600 yuan in five years continuously from 2015 to 2019 in ten thousand mu of strawberries is increased, the agriculture is increased, the income of farmers is increased, and the effects of stranded and young healthy items of farmers in the Huaxi area are obviously achieved.
Example 5: yield increasing effect on green Chinese medicinal materials
1) The plant immune regulator comprises the following components in percentage by weight: 8.5 parts of L-ascorbic acid and 91.5 parts of a filler;
2) the application method comprises the following steps: 1500-2000 times of liquid spraying;
3) a place: 2015 + 2019, Guizhou province, Liudianshui city, Water City county, Polygonatum sibiricum Red, bletilla striata, Dendrobium officinale and other Chinese medicinal material bases in a waxberry forest farm;
4) the effect is as follows: the method has the advantages that the visual effect of continuously and accurately implementing the immune regulator-8.5% ascorbic acid water agent in traditional Chinese medicinal materials such as rhizoma polygonati, bletilla striata and dendrobium officinale in 2015-2019 is obvious, the rhizoma polygonati is taken as an example planting area, the average 1 jin/mu of a treatment area is excavated in three years, the average 33jin/mu in one year is calculated by 8-10 yuan, and the annual output value is 26664-33330 yuan. The average of the control area is 0.85 jin/mu, the average of the control area is 2833 jin/mu in one year, the annual output value is 22664 and 28330 jin/mu calculated by 8-10 yuan per jin, and the yield is increased by 15%. Compared with the control area, the yield per mu of the treatment area is 0.4-0.5 ten thousand yuan more, the yield per mu of the treatment area is 20-25 ten thousand yuan more in total for 50 mu of the five-year yield, and the ecological benefit, the social benefit and the economic benefit are extremely remarkable and are deeply welcomed by pesticide farmers;
5) the second effect is that: 50 mu of spring pseudo-ginseng in Wenshan mountain science and technology in Yunnan province, continuously and accurately implementing a green prevention and control preparation, namely an immunomodulator-8.5% ascorbic acid water agent, from 2015 + 2019, effectively reducing and preventing and controlling the damage of root rot, gray mold, black spot and powdery mildew of the spring pseudo-ginseng, excavating three years for 3-7 years, taking an example that the area is treated every year, wherein the acre yield of the dry spring pseudo-ginseng is 150 + 200kg, and the acre yield value is 1.5-2.0 ten thousand yuan; the yield per mu of the control area is 120-140kg, the yield per mu value is 1.2-1.4.0 ten thousand yuan, the yield is increased by 20-30% compared with the control area, the yield per mu of the control area is 30-60 ten thousand yuan in total for 50 mu of five-year production, and the ecological benefit, the social benefit and the economic benefit are extremely obvious and are deeply welcomed by drug farmers.
Claims (6)
1. A plant immunomodulator, characterized by: comprises the following components in parts by weight:
5-40 parts of L-ascorbic acid and 60-95 parts of a filler.
2. The plant immunomodulator according to claim 1, which comprises the following components in parts by weight:
7-10 parts of L-ascorbic acid and 90-93 parts of a filler.
3. The plant immunomodulator according to claim 1, which comprises the following components in parts by weight:
8.5 parts of L-ascorbic acid and 91.5 parts of filler.
4. The application of the plant immunomodulator in the growth of green plants is shown in the following (A) and (B), and is characterized in that:
(A) increasing the yield;
(B) preventing and controlling virus diseases of green plants.
5. The use of a plant immunomodulator according to claim 4 wherein the control of viral diseases in green plants comprises:
(b1) preventing and alleviating potyvirus;
(b2) preventing and alleviating tobacco potato virus Y;
(b3) preventing and relieving black shank;
(b4) preventing and relieving bacterial wilt.
6. Use of a plant immune modulator according to claim 4 or 5, characterized in that it is used in particular in:
1) grain crops including potatoes, rice, upland rice, wheat, corn;
2) commercial crops including tobacco, tea, peanut, rape;
3) vegetables including Chinese cabbage, caulis et folium Brassicae Capitatae, herba Apii Graveolentis, herba Alii Fistulosi, fructus Solani Melongenae, and Capsici fructus;
4) melons, including watermelon and cucumber;
5) fruits including strawberry, grape, peach, plum, apple, and orange;
6) green Chinese medicinal materials including radix Notoginseng, rhizoma Polygonati, radix Ginseng, radix Panacis Quinquefolii, and rhizoma Polygoni Multiflori.
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| CN202010317764.9A CN113519524A (en) | 2020-04-21 | 2020-04-21 | Plant immunomodulator and application thereof |
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Citations (6)
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| CN85107690A (en) * | 1985-10-22 | 1987-04-29 | 北京有色金属研究总院 | Synthesizing of the titanium that plant-growth regulator is used-xitix solid chemical compound |
| CN103420707A (en) * | 2012-05-24 | 2013-12-04 | 陈广 | Cold-resistant agent for plant |
| CN104719341A (en) * | 2015-02-03 | 2015-06-24 | 凯姆勒吸水材料（天津）有限公司 | Compounded plant growth regulator capable of delaying blooming period of fruit tree |
| CN104886053A (en) * | 2015-05-14 | 2015-09-09 | 昆明理工大学 | Application of ascorbic acid to improvement of plant photosynthesis efficiency |
| CN106665573A (en) * | 2017-01-09 | 2017-05-17 | 张和林 | Biopesticide containing ascorbic acid and preparation method thereof |
| CN107568213A (en) * | 2017-09-08 | 2018-01-12 | 黄伟 | A kind of ascorbic acid soluble powder granula and its preparation method and application |
-
2020
- 2020-04-21 CN CN202010317764.9A patent/CN113519524A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85107690A (en) * | 1985-10-22 | 1987-04-29 | 北京有色金属研究总院 | Synthesizing of the titanium that plant-growth regulator is used-xitix solid chemical compound |
| CN103420707A (en) * | 2012-05-24 | 2013-12-04 | 陈广 | Cold-resistant agent for plant |
| CN104719341A (en) * | 2015-02-03 | 2015-06-24 | 凯姆勒吸水材料（天津）有限公司 | Compounded plant growth regulator capable of delaying blooming period of fruit tree |
| CN104886053A (en) * | 2015-05-14 | 2015-09-09 | 昆明理工大学 | Application of ascorbic acid to improvement of plant photosynthesis efficiency |
| CN106665573A (en) * | 2017-01-09 | 2017-05-17 | 张和林 | Biopesticide containing ascorbic acid and preparation method thereof |
| CN107568213A (en) * | 2017-09-08 | 2018-01-12 | 黄伟 | A kind of ascorbic acid soluble powder granula and its preparation method and application |
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