CN113508774A

CN113508774A - Automatic system suitable for marine organism culture

Info

Publication number: CN113508774A
Application number: CN202110441657.1A
Authority: CN
Inventors: 付萍; 程玲; 李凡; 马元庆; 宋秀凯; 王玮云; 王宁; 刘爱英; 邢红艳
Original assignee: Shandong Marine Resource and Environment Research Institute
Current assignee: Shandong Marine Resource and Environment Research Institute
Priority date: 2021-04-23
Filing date: 2021-04-23
Publication date: 2021-10-19

Abstract

The invention discloses an automatic system suitable for marine organism culture, which comprises a culture pond, a salinity pH control device, a replenishing device, a water changing device and a gas replenishing device. The salinity and pH control device can respectively monitor the change of salinity and pH in the culture pond in real time, when the salinity and the pH in the culture pond are not consistent with set values, the water pump is started through signal conduction to respectively convey pure water and buffer solution to the culture pond, and the conveying is stopped until the salinity and the pH in the culture pond are recovered to the set values; the device such as supply, trade water, tonifying qi, light-resistant, illumination, filtration can satisfy the needs of different or complicated marine organism culture environment of setting, integrates multiple equipment, replaces each unit independent operation of tradition, reaches centralized management, the purpose of centralized operation, simplifies culture apparatus by a wide margin.

Description

Automatic system suitable for marine organism culture

Technical Field

The invention relates to the technical field of marine organism culture devices, in particular to an automatic system suitable for marine organism culture.

Background

The laboratory culture of marine organisms is an important means for carrying out scientific research. In the traditional laboratory culture, seawater evaporation often occurs to cause overhigh environmental salinity and even dryness; feces excreted by the cultured organisms and residual baits cause the problems of pH change of the culture environment and the like. Because real-time monitoring and timely adjustment cannot be realized, cultured organisms are easily damaged or die, and the experimental results have large deviation and even experimental failure comes from beginning to end, so that the difficulty is caused for scientific researchers to smoothly carry out experimental research.

In addition, the traditional culture equipment is usually formed into units, and is generally combined and assembled temporarily aiming at the needs of biological culture environment, so that more time and space are occupied; often, since one unit device is not provided, the work cannot be carried out.

Disclosure of Invention

The invention aims to provide an automatic system suitable for marine organism culture, which solves the problems in the prior art, can meet the requirements of different culture organism growth environments, greatly simplifies culture equipment, and achieves the aims of centralized management and centralized operation.

In order to achieve the purpose, the invention provides the following scheme: the invention provides an automatic system suitable for marine organism culture, which comprises a culture pond, a salinity pH control device, a replenishing device, a water changing device and a gas replenishing device, wherein the culture pond is provided with an inner pond and an outer pond; the salinity pH control device is electrically connected with a determinator arranged in the inner pond through a signal line I, and the salinity pH control device controls a water pump to convey pure water or buffer solution into the culture pond according to information fed back by the determinator; the feeding device comprises a feeding container and a feeding pipe, and feeding liquid contained in the feeding container supplies feed to the culture pond through the feeding pipe; the water changing device comprises a water changing container and a water changing pipe, and the water changing container is communicated with the culture pond through the water changing pipe; the air supplementing device comprises an air supplementing pipe and an air supplementing valve, the air supplementing valve is arranged on the air supplementing pipe, one end of the air supplementing pipe is connected with an air source, and the other end of the air supplementing pipe is communicated with the culture pond.

Preferably, the bottom of the inner tank and the bottom of the outer tank are both provided with a water filtering hole and a water outlet hole; a filter screen and a first plug are arranged at the position of the water filtering hole; the water outlet hole is provided with a second plug.

Preferably, the salinity pH control device is provided with a display screen, a number key, an on-off key and an up-down key which are electrically connected with a control computer.

Preferably, the salinity and pH control device is connected with a second signal wire, the second signal wire is connected with a first water pump, the first water pump is connected with a pure water pipe, and the pure water pipe supplies liquid to the inner tank after penetrating through a pure water pipe hole in the outer tank wall; the salinity pH control device is also connected with a signal wire III, the signal wire III is connected with a water pump II, the water pump II is connected with a buffer liquid pipe, and the buffer liquid pipe passes through a buffer liquid pipe hole in the outer pond wall and then supplements buffer liquid for the inner pond; the first water pump is arranged in the first container, the second water pump is arranged in the second container, the first container and the second container are connected into an integral container, and a sealed partition plate is arranged between the first container and the second container.

Preferably, the wall of the supply container cup is provided with scales, and a supply valve is arranged on the supply pipe; and the other end of the supply pipe penetrates through a supply pipe hole arranged on the outer pool wall and then supplies supply liquid to the culture pool.

Preferably, the cup wall of the water changing container is provided with scales, and the water changing pipe is connected with a water changing valve; the other end of the water changing pipe penetrates through a water changing pipe hole formed in the outer pool wall and then is communicated with the culture pool.

Preferably, the top of the opening end of the inner tank is provided with a groove, and the signal line I, the pure water pipe, the buffer liquid pipe, the supply pipe, the water changing pipe and the air supply pipe are all placed in the inner tank through the groove on the inner tank; and scales are arranged on the inner tank wall.

Preferably, the outer tank comprises an outer tank outer wall and an outer tank inner wall, an outer tank outer cover is arranged at the top of the outer tank outer wall, and an outer tank inner cover is arranged at the top of the outer tank inner wall; the top of the inner cover of the outer tank is provided with a loose and porous transverse lattice; fluorescent lamps are fixed on the transverse grids; an independent and detachable filter screen is arranged below the inner cover of the outer tank; a control device for controlling the fluorescent lamp is arranged on the outer side of the edge of the inner cover of the outer pool; the control device is provided with a switch, a display screen and an up-down key.

Preferably, an air supply branch pipe is arranged on an air supply pipe in the inner pool; the air supply branch pipe is provided with an air supply micro-pipe; the air supply branch pipe and the air supply micro-pipe are both arranged in a U-shaped groove on the inner wall of the inner tank.

Compared with the prior art, the invention has the following beneficial technical effects:

according to the automatic system suitable for marine organism culture, the salinity and pH control device can monitor the change of salinity and pH in a culture environment in real time, when the salinity and pH deviate from a set value, the water pump is started to pump pure water and buffer solution in time, and when the salinity and pH in the culture pond reach the set value, the water pump stops working. The invention integrates devices of supplying, changing water, supplying air, avoiding light, illuminating, filtering air and the like, can meet the requirements of different growth environments of cultured organisms, greatly simplifies culture equipment, and achieves the aims of centralized management and centralized operation.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

FIG. 1 is a schematic diagram of an automated system suitable for marine organism culture;

2a, 2b and 2c in FIG. 2 are schematic structural diagrams of a make-up valve, a water change valve and a gas make-up valve respectively;

FIG. 3 is a schematic structural view of the outer cover of the outer tank of the present invention;

FIG. 4 is a schematic view of the inner cover structure of the outer tank of the present invention;

FIG. 5 is a schematic view of the structure of the air supplement pipe of the present invention;

FIG. 6 is a schematic view of the hole-in-glass wall and the sealing latex of the present invention;

FIG. 7 is a schematic view of a filter screen according to the present invention;

wherein, 1, culturing a pond; 2 salinity pH control device; 3 a supply device; 4, a water changing device; 5 an air supply device; 6, an inner pool; 7, an outer pool; 8, water filtering holes; 9, filtering by using a filter screen; 10 a first plug; 11 water outlet holes; 12 a second plug; 13 a display screen; 14 number keys; 15 on-off key; 16 up and down keys; 17 a first signal line; 18 a measuring device; 19 assay well; 20 sealing the latex; 21 a second signal line; 22, a first water pump; 23 pure water pipes; 24 holes of pure water pipes; 25 sealing the latex; 26 a third signal line; 27, a second water pump; 28 buffer liquid tube; 29 buffer tube holes; 30 sealing the latex; 31 a first container; 32, a second container; 34 a partition plate; 35 a supply container; 36 scales; 37 a supply pipe; 38 a supply hole; 39 sealing the latex; 40 a supply valve; 41 make-up valve nut; 42 hexagon; 43 a cylinder; 44 threads; 45 of a combined body; 46 thread; 47 supply tube holes; 48 sealing the latex; 49 replacing the water container; 50 scales; 51 water changing pipe; 52 water changing holes; 53 sealing the latex; 54 water changing valve; 55, changing a water valve nut; 56 hexagon; a 57 cylinder; 58 threads; 59 a combined body; 60 threads; 61 changing the water pipe hole; 62 sealing the latex; 63 air supplement pipe; 64 air supplement valve; 65 a gas compensation valve nut; 66 hexagon; 67 a cylinder; 68 threads; 69 a combination body; 70, thread; 71 changing the water pipe hole; 72 sealing the latex; 73 grooves; 74 scales; 75 outer wall of the outer tank; 76 outer tank inner wall; 77 outer cover of the outer pool; 78 the lower part of the outer cover of the outer tank; 79 emulsion ring; 80 outer pool inner cover; 81 horizontal grids; 82 fluorescent lamps; 83 a control device; an 84 switch; 85 display screen; 86 up-down keys; 87 the lower part of the inner cover of the outer tank; 88 latex ring; 89 air supply branch pipes; 90 air-replenishing microtubules; 91U-shaped groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.

As shown in FIGS. 1-7, the present embodiment provides an automated system for marine organism cultivation, which comprises a cultivation pond 1, a salinity pH control device 2, a replenishment device 3, a water exchange device 4 and a gas replenishment device 5.

Specifically, the culture tank 1 is provided with an inner tank 6 and an outer tank 7; the inner tank 6 and the outer tank 7 are provided with water filtering holes 8; a filter screen 9 and a first plug 10 are arranged at the water filtering hole 8; the inner tank 6 and the outer tank 7 are also provided with water outlet holes 11; the water outlet hole 11 is provided with a second plug 12.

The salinity pH control device 2 is provided with a display screen 13, a number key 14, an on-off key 15 and an up-down key 16; the salinity pH control device 2 is connected with a signal line I17; the first signal wire 17 is connected with a measurer 18; the measuring device 18 passes through a measuring hole 19 on the wall of the outer pool 7; the measuring hole 19 is connected with a closed latex 20; the salinity pH control device 2 is connected with a second signal wire 21; the signal wire 21 is connected with a first water pump 22; the first water pump 22 is connected with a pure water pipe 23; the pure water pipe 23 passes through a pure water pipe hole 24 on the wall of the outer tank 7; the pure water pipe hole 24 is provided with closed latex 25; the salinity pH control device 2 is connected with a third signal wire 26; the signal wire III 26 is connected with the water pump II 27; the second water pump 27 is connected with a buffer liquid pipe 28; the buffer liquid pipe 28 passes through a buffer liquid pipe hole 29 on the wall of the outer tank 7; the buffer tube hole 29 is provided with a closed latex 30; the first water pump 22 is arranged in the first container 31; the second water pump 27 is arranged in the second container 32; the first container 31 and the second container 32 are connected into an integral container; two partition boards 34 are arranged in the middle to completely separate the first container 31 from the second container 32.

Further, the replenishing device 3 is provided with a replenishing container 35; the wall of the supply container is provided with scales 36; the supply device 3 is provided with a supply pipe 37; the supply pipe 37 passes through a supply hole 38 on the cup wall of the supply container 35; the supply hole 38 is provided with closed latex 39; the supply pipe 37 is connected with a supply valve 40; the supply valve 40 is provided with a supply valve nut 41; the wide surface of the supply valve nut 41 is a hexagon 42; the supply valve nut 41 is provided with a cylinder 43; the cylinder 43 is provided with a thread 44; the supply valve 40 is provided with a combined body 45; the left and right of the combination body 45 are connected with a supply pipe 37; the combination body 45 is provided with a screw thread 46 for combining with the screw thread 44; the supply pipe 37 passes through a supply pipe hole 47 arranged on the wall of the outer tank 7; the supply pipe hole 47 is provided with a closed latex 48.

The water changing device 4 is provided with a water changing container 49; the cup wall of the water changing container 49 is provided with scales 50; the water changing device 4 is provided with a water changing pipe 51; the water changing pipe 51 passes through a water changing hole 52 on the cup wall of the water changing container 49; the water changing hole 52 is provided with closed latex 53; the water changing pipe is connected with a water changing valve 54; the water changing valve 54 is provided with a water changing valve nut 55; the wide surface of the water changing valve nut 55 is hexagonal 56; the water change valve nut 55 is provided with a cylinder 57; the cylinder 57 is provided with a thread 58; the water changing valve 54 is provided with a water changing valve nut combination body 59; the combined body 59 is connected with the water changing pipe 51 left and right; the combination 59 is provided with a thread 60 for combining with the thread 58; the water changing pipe 51 passes through a water changing pipe hole 61 arranged on the wall of the outer tank 7; the water changing pipe hole 61 is provided with a sealed latex 62.

The air supply device 5 is provided with an air supply pipe 63; the air supply pipe 63 is connected with an air supply valve 64; the air supply valve 64 is provided with an air supply valve nut 65; the wide surface of the air supplement valve nut 65 is hexagonal 66; the air supplement valve nut 65 is provided with a cylinder 67; the cylinder 67 is provided with a thread 68; the air supply valve 64 is provided with a combined body 69; the left and right of the combination body 69 are connected with the air supplement pipe 63; the combination body 69 is provided with a screw thread 70 for combining with the screw thread 68; the air supply pipe 63 passes through an air supply pipe hole 71 arranged on the wall of the outer pool 7; the air-supply pipe hole 71 is provided with a closed latex 72.

In this embodiment, the top of the open end of the inner tank 6 is provided with a groove 73, and the signal line one 17, the pure water pipe 23, the buffer liquid pipe 28, the supply pipe 37, the water changing pipe 51 and the air supply pipe 63 are placed in the inner tank 6 through the groove 73 on the inner tank 6; the wall of the inner pool 6 is provided with scales 74;

the outer tank 7 is provided with an outer tank wall 75 and an outer tank inner wall 76; the outer wall 75 of the outer tank is connected with an outer tank cover 77, and the outer tank cover 77 is connected with the lower part 78 of the outer tank cover; the lower part 78 of the outer cover of the outer pool is connected with a latex ring 79; the inner wall 76 of the outer tank is connected with an inner cover 80 of the outer tank; the top of the inner cover 80 of the outer tank is provided with a loose and porous transverse lattice 81; the transverse grids 81 fix the fluorescent lamp 82; an independently detachable filter screen 92 is arranged below the outer tank inner cover 80, the filter screen 92 is designed into three layers, the diameter of the filter screen is larger than the diameter of an opening on the inner wall of the outer tank and smaller than the diameter of an opening on the outer wall of the outer tank, and the filter screen 92 can effectively filter bacteria in air (similar to a surgical medical mask, the filtering efficiency is more than or equal to 95%); the fluorescent lamp 82 is provided with a control device 83 at the outer side of the edge of the inner cover 80 of the outer tank; the control device 83 is provided with a switch 84, a display screen 85 and an up-down key 86; the outer inner tank cover 80 is connected with the lower part 87 of the outer inner tank cover, and the lower part 87 of the outer inner tank cover is connected with the latex ring 88; the culture pond 1 and the outer cover 77 of the outer pond are both brown glass bottles.

In this embodiment, the air supply pipe 63 is provided with an air supply branch pipe 89 in the inner tank 6; the air supply branch pipe 89 is provided with an air supply micro-pipe 90; the air supply branch pipe 89 and the air supply micro-pipe 90 are both arranged in a U-shaped groove 91 on the inner wall of the inner pool 6.

The automatic system suitable for marine organism culture in the invention has the following specific use method:

firstly, salinity and pH value are set in a salinity and pH control device 2, the salinity and pH value in a culture pond 1 is monitored in real time through a determinator 18, when the salinity in the culture pond 1 is increased, a first water pump 22 is started through signal conduction to pump pure water into the culture pond 1, and when the salinity in the culture pond 1 reaches a preset value, the water pumping is stopped; when the pH value of the culture pond is changed due to the excrement of the cultured organisms and food residues, the second water pump 27 is started through signal conduction to pump the buffer solution into the culture pond 1, and when the pH value in the culture pond 1 reaches a preset value, the pumping is stopped.

In the water changing process, firstly ensuring that the water changing valve 54 is closed, recording water level scales in the culture pond 1, opening the first plug 10 of the water filtering hole 8, intercepting organisms in the culture pond 1 while discharging water, opening the water changing valve 54, inputting seawater into the culture pond 1 by utilizing the pressure difference between the water changing container 49 and the culture pond 1, and enabling the water level to reach the target scales; the water supply device 4 is replaced by the supply device 3. According to the requirement of the culture environment, the air supply device 5 can be used for carrying out CO treatment on the culture pond 1₂、O₂Or other gases are supplied, the gases enter the culture pond 1 through the air supplementing pipe 63, the supplementing branch pipe 89 and the supplementing micro-pipe 90 and are fixed through a U-shaped groove 91 on the wall of the inner pond 6; when the culture pond 1 is replenished, the air replenishing device 5 can be opened, and the nutrient can be favorably dispersed in the culture pond 1. The transverse lattices 81 and the fluorescent lamp 82 are designed on the outer inner tank cover 80, and the loose and porous transverse lattices 81 can not block the free circulation of the culture tank 1 and the outside air when the outer inner tank cover is covered, and can play a role in fixing the fluorescent lamp 82.

The culture pond 1 is designed into two layers (an inner pond 6 and an outer pond 7), and the inner pond 6 and the outer pond 7 are both designed into brown, and the design mode mainly considers the requirements of culturing organisms on sealing, light shielding and the like. Cultivate pond 1 and set up to brown glass bottle, get into the signal line in cultivateing pond 1, each kind of pipe all passes the outer pond 7 of cultivateing pond 1, sealed latex can guarantee inclosed, but can avoid having some light to see through, outer pond 7 still can not satisfy the needs of light-resistant, consequently, inner pond 6 has been designed, with the signal line, pond 6 in various pipes pass through the recess 73 on inner pond 6 upper portion and get into, inner pond 6 designs into brown glass bottle, the effect of light-resistant has been strengthened when further remedying outer pond 7 and can't reach complete light-resistant needs. When the culture environment needs to be sealed and light-proof, the outer cover 77 (brown glass bottle) of the outer pool is covered; if the illumination is needed for several hours on the basis, before the outer tank outer cover 77 is covered, the outer tank inner cover 80 is covered, the fluorescent lamps 82 are opened, the number of the fluorescent lamps 82 is selected to be started, and the illumination time is set; when the culture environment needs open culture but bacteria in the air need to be filtered to a certain extent, the outer tank cover 77 is taken down, the filter screen 92 is placed at the top opening end of the inner wall of the outer tank 7 before the outer tank inner cover 80 is covered, and then the outer tank inner cover 80 is covered; when the culture environment is open, and normal illumination culture is performed, the outer cover 77 of the outer tank is taken down, the inner cover 80 of the outer tank is covered, the fluorescent lamp 82 is opened, and the illumination time is set. When the water level scale of the culture pond 1 and the biological culture condition are observed, the observation can be carried out by turning on the fluorescent lamp 82, so that the difficulty of observing caused by the brown glass ware is avoided.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein, and any reference signs in the claims are not intended to be construed as limiting the claim concerned.

The principle and the implementation mode of the invention are explained by applying a specific example, and the description of the embodiment is only used for helping to understand the method and the core idea of the invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, the specific embodiments and the application range may be changed. In view of the above, the present disclosure should not be construed as limiting the invention.

Claims

1. An automatic system suitable for marine organism culture, which is characterized in that: the device comprises a culture pond, a salinity pH control device, a replenishing device, a water changing device and a replenishing device, wherein the culture pond is provided with an inner pond and an outer pond; the salinity pH control device is electrically connected with a determinator arranged in the inner pond through a signal line I, and the salinity pH control device controls a water pump to convey pure water or buffer solution into the culture pond according to information fed back by the determinator; the feeding device comprises a feeding container and a feeding pipe, and feeding liquid contained in the feeding container supplies feed to the culture pond through the feeding pipe; the water changing device comprises a water changing container and a water changing pipe, and the water changing container is communicated with the culture pond through the water changing pipe; the air supplementing device comprises an air supplementing pipe and an air supplementing valve, the air supplementing valve is arranged on the air supplementing pipe, one end of the air supplementing pipe is connected with an air source, and the other end of the air supplementing pipe is communicated with the culture pond.

2. Automated system suitable for marine organism culture according to claim 1, characterized in that: the bottoms of the inner tank and the outer tank are provided with water filtering holes and water outlet holes; a filter screen and a first plug are arranged at the position of the water filtering hole; the water outlet hole is provided with a second plug.

3. Automated system suitable for marine organism culture according to claim 1, characterized in that: and the salinity pH control device is provided with a display screen, a number key, an on-off key and an up-down key which are electrically connected with a control computer.

4. Automated system suitable for marine organism culture according to claim 1, characterized in that: the salinity and pH control device is connected with a second signal wire, the second signal wire is connected with a first water pump, the first water pump is connected with a pure water pipe, and the pure water pipe supplies liquid to the inner tank after penetrating through a pure water pipe hole in the wall of the outer tank; the salinity pH control device is also connected with a signal wire III, the signal wire III is connected with a water pump II, the water pump II is connected with a buffer liquid pipe, and the buffer liquid pipe supplies buffer liquid to the inner pond after penetrating through a buffer liquid pipe hole in the wall of the outer pond; the first water pump is arranged in the first container, the second water pump is arranged in the second container, the first container and the second container are connected into an integral container, and a sealed partition plate is arranged between the first container and the second container.

5. Automated system suitable for marine organism culture according to claim 1, characterized in that: scales are arranged on the cup wall of the supply container, and a supply valve is arranged on the supply pipe; and the other end of the supply pipe penetrates through a supply pipe hole arranged on the outer pool wall and then supplies supply liquid to the culture pool.

6. Automated system suitable for marine organism culture according to claim 1, characterized in that: scales are arranged on the cup wall of the water changing container, and a water changing valve is connected to the water changing pipe; the other end of the water changing pipe penetrates through a water changing pipe hole formed in the outer pool wall and then is communicated with the culture pool.

7. Automated system suitable for marine organism culture according to claim 1, characterized in that: a groove is formed in the top of the opening end of the inner tank, and the signal line I, the pure water pipe, the buffer liquid pipe, the supply pipe, the water changing pipe and the air supply pipe are all placed into the inner tank through the groove in the inner tank; the inner pool wall is provided with scales.

8. Automated system suitable for marine organism culture according to claim 1, characterized in that: the outer pool comprises an outer pool outer wall and an outer pool inner wall, an outer pool outer cover is arranged at the top of the outer pool outer wall, and an outer pool inner cover is arranged at the top of the outer pool inner wall; the top of the inner cover of the outer tank is provided with a loose and porous transverse lattice; fluorescent lamps are fixed on the transverse grids; an independent and detachable filter screen is arranged below the inner cover of the outer tank; a control device for controlling the fluorescent lamp is arranged on the outer side of the edge of the inner cover of the outer pool; the control device is provided with a switch, a display screen and an up-down key.

9. Automated system suitable for marine organism culture according to claim 1, characterized in that: an air supply branch pipe is arranged on the air supply pipe in the inner pool; the air supply branch pipe is provided with an air supply micro-pipe; the air supply branch pipe and the air supply micro-pipe are both arranged in a U-shaped groove on the inner wall of the inner tank.