CN113507835A - 内生孢子展示平台、产品和方法 - Google Patents

内生孢子展示平台、产品和方法 Download PDF

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Publication number
CN113507835A
CN113507835A CN201980089106.4A CN201980089106A CN113507835A CN 113507835 A CN113507835 A CN 113507835A CN 201980089106 A CN201980089106 A CN 201980089106A CN 113507835 A CN113507835 A CN 113507835A
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CN
China
Prior art keywords
bacillus
brevibacillus
fusion protein
ile
polypeptide
Prior art date
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Pending
Application number
CN201980089106.4A
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English (en)
Chinese (zh)
Inventor
B·L·戈洛姆
F·A·菲卡拉
R·萨拉姆扎德
D·柯蒂斯
K·蒂普顿
D·艾利
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ginkgo Bioworks Inc
Original Assignee
Bayer CropScience LP
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Filing date
Publication date
Application filed by Bayer CropScience LP filed Critical Bayer CropScience LP
Publication of CN113507835A publication Critical patent/CN113507835A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Environmental Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
CN201980089106.4A 2018-11-15 2019-11-15 内生孢子展示平台、产品和方法 Pending CN113507835A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201862767997P 2018-11-15 2018-11-15
US201862768063P 2018-11-15 2018-11-15
US201862768077P 2018-11-15 2018-11-15
US62/768,077 2018-11-15
US62/767,997 2018-11-15
US62/768,063 2018-11-15
PCT/US2019/061648 WO2020102642A2 (en) 2018-11-15 2019-11-15 Endospore display platforms, products and methods

Publications (1)

Publication Number Publication Date
CN113507835A true CN113507835A (zh) 2021-10-15

Family

ID=68982426

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980089106.4A Pending CN113507835A (zh) 2018-11-15 2019-11-15 内生孢子展示平台、产品和方法

Country Status (13)

Country Link
US (1) US20220002736A1 (https=)
EP (1) EP3879982A2 (https=)
JP (1) JP2022507370A (https=)
KR (1) KR20210091261A (https=)
CN (1) CN113507835A (https=)
AU (1) AU2019381797A1 (https=)
BR (1) BR112021008927A2 (https=)
CA (1) CA3119778A1 (https=)
CL (1) CL2021001251A1 (https=)
MX (1) MX2021005759A (https=)
PY (1) PY1996572A (https=)
UY (1) UY38475A (https=)
WO (1) WO2020102642A2 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116676212A (zh) * 2023-04-27 2023-09-01 四川农业大学 一株Brevibacillus aydinogluensis PMBT001及其应用
CN119432655A (zh) * 2024-11-07 2025-02-14 沈阳农业大学 一株巴基斯坦赖氨酸芽胞杆菌Sneb2537与一种能够防治根结线虫病的制剂及其制备方法和应用

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112029685B (zh) * 2020-09-02 2022-03-25 浙江工业大学 一种耐硼赖氨酸芽孢杆菌及其在混凝土裂缝修复中的应用
AU2022275552A1 (en) * 2021-05-21 2023-11-30 Ginkgo Bioworks, Inc. Intergeneric endospore display platforms, products and methods
CN120082485B (zh) * 2025-04-29 2025-09-02 中国农业科学院草原研究所 一株短芽孢杆菌9-1及其在微生物菌肥中的应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002046388A1 (en) * 2000-12-08 2002-06-13 Genofocus Co., Ltd. Method for expression of proteins on spore surface
CN104507311A (zh) * 2012-05-30 2015-04-08 拜尔农作物科学股份公司 包含生物防治剂和杀虫剂的组合物
CN104519742A (zh) * 2012-05-30 2015-04-15 拜尔农作物科学股份公司 包括生物防治剂和杀虫剂的组合物
US20160338360A1 (en) * 2013-12-24 2016-11-24 Indigo Ag, Inc. Plants containing beneficial endophytes

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US4272417A (en) 1979-05-22 1981-06-09 Cargill, Incorporated Stable protective seed coating
US4245432A (en) 1979-07-25 1981-01-20 Eastman Kodak Company Seed coatings
US4808430A (en) 1987-02-27 1989-02-28 Yazaki Corporation Method of applying gel coating to plant seeds
US5876739A (en) 1996-06-13 1999-03-02 Novartis Ag Insecticidal seed coating
US6503904B2 (en) 1998-11-16 2003-01-07 Syngenta Crop Protection, Inc. Pesticidal composition for seed treatment
US6660690B2 (en) 2000-10-06 2003-12-09 Monsanto Technology, L.L.C. Seed treatment with combinations of insecticides
US20020134012A1 (en) 2001-03-21 2002-09-26 Monsanto Technology, L.L.C. Method of controlling the release of agricultural active ingredients from treated plant seeds
US9133251B2 (en) 2008-02-22 2015-09-15 The Curators Of The University Of Missouri Bacillus based delivery system and methods of use
US9573980B2 (en) * 2013-03-15 2017-02-21 Spogen Biotech Inc. Fusion proteins and methods for stimulating plant growth, protecting plants from pathogens, and immobilizing Bacillus spores on plant roots
RU2017113002A (ru) * 2014-09-17 2018-10-17 Байер Кропсайенс Лп Композиции, содержащие рекомбинантные клетки bacillus и фунгицид
CA3016922A1 (en) * 2016-03-16 2017-09-21 Spogen Biotech Inc. Fusion proteins, recombinant bacteria, and exosporium fragments for animal health and aquaculture

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002046388A1 (en) * 2000-12-08 2002-06-13 Genofocus Co., Ltd. Method for expression of proteins on spore surface
CN104507311A (zh) * 2012-05-30 2015-04-08 拜尔农作物科学股份公司 包含生物防治剂和杀虫剂的组合物
CN104519742A (zh) * 2012-05-30 2015-04-15 拜尔农作物科学股份公司 包括生物防治剂和杀虫剂的组合物
US20160338360A1 (en) * 2013-12-24 2016-11-24 Indigo Ag, Inc. Plants containing beneficial endophytes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BRIAN M. THOMPSON: "Targeting of the BclA and BclB proteins to the Bacillus anthracis spore surface", MOLECULAR MICROBIOLOGY, vol. 70, no. 2, XP002788681, DOI: 10.1111/j.1365-2958.2008.06420.x *
PPA85733.1: "collagen-like triple helix repeat-containing protein, partial [Brevibacillus laterosporus]", GENBANK *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116676212A (zh) * 2023-04-27 2023-09-01 四川农业大学 一株Brevibacillus aydinogluensis PMBT001及其应用
CN116676212B (zh) * 2023-04-27 2024-01-26 四川农业大学 一株Brevibacillus aydinogluensis PMBT001及其应用
CN119432655A (zh) * 2024-11-07 2025-02-14 沈阳农业大学 一株巴基斯坦赖氨酸芽胞杆菌Sneb2537与一种能够防治根结线虫病的制剂及其制备方法和应用

Also Published As

Publication number Publication date
AU2019381797A1 (en) 2021-06-10
WO2020102642A3 (en) 2020-07-30
UY38475A (es) 2020-06-30
EP3879982A2 (en) 2021-09-22
WO2020102642A8 (en) 2021-02-25
MX2021005759A (es) 2021-08-11
JP2022507370A (ja) 2022-01-18
CA3119778A1 (en) 2020-05-22
PY1996572A (es) 2023-01-17
BR112021008927A2 (pt) 2021-08-17
US20220002736A1 (en) 2022-01-06
WO2020102642A2 (en) 2020-05-22
KR20210091261A (ko) 2021-07-21
CL2021001251A1 (es) 2021-11-19

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Effective date of registration: 20230222

Address after: Massachusetts

Applicant after: Ginkgo Biological Products Co.

Address before: Missouri, USA

Applicant before: BAYER CROPSCIENCE L.P.

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Application publication date: 20211015