CN113498434A - 间充质干细胞膜片及其用途 - Google Patents
间充质干细胞膜片及其用途 Download PDFInfo
- Publication number
- CN113498434A CN113498434A CN202080000065.XA CN202080000065A CN113498434A CN 113498434 A CN113498434 A CN 113498434A CN 202080000065 A CN202080000065 A CN 202080000065A CN 113498434 A CN113498434 A CN 113498434A
- Authority
- CN
- China
- Prior art keywords
- mesenchymal stem
- stem cell
- stem cells
- umbilical cord
- heart
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000002901 mesenchymal stem cell Anatomy 0.000 title claims abstract description 174
- 239000012528 membrane Substances 0.000 title claims description 26
- 210000003954 umbilical cord Anatomy 0.000 claims abstract description 45
- 238000000034 method Methods 0.000 claims abstract description 38
- 206010019280 Heart failures Diseases 0.000 claims abstract description 35
- 201000010099 disease Diseases 0.000 claims abstract description 25
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 25
- 210000005003 heart tissue Anatomy 0.000 claims abstract description 12
- 239000000203 mixture Substances 0.000 claims abstract description 4
- 210000004027 cell Anatomy 0.000 claims description 67
- 210000001519 tissue Anatomy 0.000 claims description 31
- 210000004379 membrane Anatomy 0.000 claims description 25
- 238000012258 culturing Methods 0.000 claims description 12
- 230000000302 ischemic effect Effects 0.000 claims description 12
- 108010067306 Fibronectins Proteins 0.000 claims description 11
- 210000002966 serum Anatomy 0.000 claims description 11
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 claims description 10
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 claims description 10
- 210000002744 extracellular matrix Anatomy 0.000 claims description 10
- 108090001007 Interleukin-8 Proteins 0.000 claims description 9
- 235000015110 jellies Nutrition 0.000 claims description 9
- 239000008274 jelly Substances 0.000 claims description 9
- 102000004127 Cytokines Human genes 0.000 claims description 8
- 108090000695 Cytokines Proteins 0.000 claims description 8
- 102000012355 Integrin beta1 Human genes 0.000 claims description 8
- 108010022222 Integrin beta1 Proteins 0.000 claims description 8
- 230000007547 defect Effects 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 8
- 108090001005 Interleukin-6 Proteins 0.000 claims description 7
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 claims description 7
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 claims description 7
- 230000006378 damage Effects 0.000 claims description 7
- 239000011159 matrix material Substances 0.000 claims description 7
- 230000001154 acute effect Effects 0.000 claims description 6
- 210000001185 bone marrow Anatomy 0.000 claims description 6
- 230000001413 cellular effect Effects 0.000 claims description 6
- 208000031229 Cardiomyopathies Diseases 0.000 claims description 5
- 208000002330 Congenital Heart Defects Diseases 0.000 claims description 5
- 208000027418 Wounds and injury Diseases 0.000 claims description 5
- 208000028831 congenital heart disease Diseases 0.000 claims description 5
- 208000029078 coronary artery disease Diseases 0.000 claims description 5
- 208000018578 heart valve disease Diseases 0.000 claims description 5
- 208000014674 injury Diseases 0.000 claims description 5
- 208000004124 rheumatic heart disease Diseases 0.000 claims description 5
- 108010035532 Collagen Proteins 0.000 claims description 4
- 102000008186 Collagen Human genes 0.000 claims description 4
- 229920001436 collagen Polymers 0.000 claims description 4
- 208000031225 myocardial ischemia Diseases 0.000 claims description 4
- 102000010970 Connexin Human genes 0.000 claims description 3
- 108050001175 Connexin Proteins 0.000 claims description 3
- 108010010803 Gelatin Proteins 0.000 claims description 3
- 102000007547 Laminin Human genes 0.000 claims description 3
- 108010085895 Laminin Proteins 0.000 claims description 3
- 108010039918 Polylysine Proteins 0.000 claims description 3
- 102100035140 Vitronectin Human genes 0.000 claims description 3
- 108010031318 Vitronectin Proteins 0.000 claims description 3
- 210000000577 adipose tissue Anatomy 0.000 claims description 3
- 210000001691 amnion Anatomy 0.000 claims description 3
- 210000004381 amniotic fluid Anatomy 0.000 claims description 3
- 210000001136 chorion Anatomy 0.000 claims description 3
- 210000003785 decidua Anatomy 0.000 claims description 3
- 210000004700 fetal blood Anatomy 0.000 claims description 3
- 229920000159 gelatin Polymers 0.000 claims description 3
- 239000008273 gelatin Substances 0.000 claims description 3
- 235000019322 gelatine Nutrition 0.000 claims description 3
- 235000011852 gelatine desserts Nutrition 0.000 claims description 3
- 210000005259 peripheral blood Anatomy 0.000 claims description 3
- 239000011886 peripheral blood Substances 0.000 claims description 3
- 210000002826 placenta Anatomy 0.000 claims description 3
- 230000003169 placental effect Effects 0.000 claims description 3
- 229920000656 polylysine Polymers 0.000 claims description 3
- 229920002714 polyornithine Polymers 0.000 claims description 3
- 108010055896 polyornithine Proteins 0.000 claims description 3
- 230000003248 secreting effect Effects 0.000 claims description 3
- 238000010008 shearing Methods 0.000 claims description 3
- 102000016359 Fibronectins Human genes 0.000 claims 2
- 101000898034 Homo sapiens Hepatocyte growth factor Proteins 0.000 claims 1
- 101001076408 Homo sapiens Interleukin-6 Proteins 0.000 claims 1
- 101000868152 Homo sapiens Son of sevenless homolog 1 Proteins 0.000 claims 1
- 230000000451 tissue damage Effects 0.000 abstract description 7
- 231100000827 tissue damage Toxicity 0.000 abstract description 7
- 241001465754 Metazoa Species 0.000 description 21
- 230000002861 ventricular Effects 0.000 description 18
- 241000699666 Mus <mouse, genus> Species 0.000 description 14
- 241000699670 Mus sp. Species 0.000 description 13
- 230000006870 function Effects 0.000 description 12
- 210000000170 cell membrane Anatomy 0.000 description 11
- 102100037362 Fibronectin Human genes 0.000 description 9
- 102000004890 Interleukin-8 Human genes 0.000 description 8
- 229940096397 interleukin-8 Drugs 0.000 description 8
- XKTZWUACRZHVAN-VADRZIEHSA-N interleukin-8 Chemical compound C([C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@@H](NC(C)=O)CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CCSC)C(=O)N1[C@H](CCC1)C(=O)N1[C@H](CCC1)C(=O)N[C@@H](C)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CC=1C=CC(O)=CC=1)C(=O)N[C@H](CO)C(=O)N1[C@H](CCC1)C(N)=O)C1=CC=CC=C1 XKTZWUACRZHVAN-VADRZIEHSA-N 0.000 description 8
- 210000005240 left ventricle Anatomy 0.000 description 8
- 239000000463 material Substances 0.000 description 8
- 102000003745 Hepatocyte Growth Factor Human genes 0.000 description 7
- 108090000100 Hepatocyte Growth Factor Proteins 0.000 description 7
- 102000004889 Interleukin-6 Human genes 0.000 description 6
- 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 description 6
- 210000000038 chest Anatomy 0.000 description 6
- 230000006698 induction Effects 0.000 description 6
- 229940100601 interleukin-6 Drugs 0.000 description 6
- 238000010186 staining Methods 0.000 description 6
- 230000004069 differentiation Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000000684 flow cytometry Methods 0.000 description 5
- 230000001737 promoting effect Effects 0.000 description 5
- 230000029058 respiratory gaseous exchange Effects 0.000 description 5
- 238000004904 shortening Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 210000000115 thoracic cavity Anatomy 0.000 description 5
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 4
- 206010016654 Fibrosis Diseases 0.000 description 4
- PIWKPBJCKXDKJR-UHFFFAOYSA-N Isoflurane Chemical compound FC(F)OC(Cl)C(F)(F)F PIWKPBJCKXDKJR-UHFFFAOYSA-N 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 230000006727 cell loss Effects 0.000 description 4
- 239000011248 coating agent Substances 0.000 description 4
- 238000000576 coating method Methods 0.000 description 4
- 239000012091 fetal bovine serum Substances 0.000 description 4
- 230000004761 fibrosis Effects 0.000 description 4
- 229960002725 isoflurane Drugs 0.000 description 4
- 238000010172 mouse model Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 210000003491 skin Anatomy 0.000 description 4
- 230000017423 tissue regeneration Effects 0.000 description 4
- -1 1640 Substances 0.000 description 3
- 102100037241 Endoglin Human genes 0.000 description 3
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 description 3
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 description 3
- NPGIHFRTRXVWOY-UHFFFAOYSA-N Oil red O Chemical compound Cc1ccc(C)c(c1)N=Nc1cc(C)c(cc1C)N=Nc1c(O)ccc2ccccc12 NPGIHFRTRXVWOY-UHFFFAOYSA-N 0.000 description 3
- 102100024616 Platelet endothelial cell adhesion molecule Human genes 0.000 description 3
- 230000002293 adipogenic effect Effects 0.000 description 3
- RGCKGOZRHPZPFP-UHFFFAOYSA-N alizarin Chemical compound C1=CC=C2C(=O)C3=C(O)C(O)=CC=C3C(=O)C2=C1 RGCKGOZRHPZPFP-UHFFFAOYSA-N 0.000 description 3
- 230000033115 angiogenesis Effects 0.000 description 3
- 238000010171 animal model Methods 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 238000010569 immunofluorescence imaging Methods 0.000 description 3
- 230000033001 locomotion Effects 0.000 description 3
- 230000002188 osteogenic effect Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 2
- 239000012981 Hank's balanced salt solution Substances 0.000 description 2
- 102100027754 Mast/stem cell growth factor receptor Kit Human genes 0.000 description 2
- 208000033774 Ventricular Remodeling Diseases 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 210000001789 adipocyte Anatomy 0.000 description 2
- 239000002870 angiogenesis inducing agent Substances 0.000 description 2
- 239000007640 basal medium Substances 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 230000008827 biological function Effects 0.000 description 2
- 210000002449 bone cell Anatomy 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 239000012228 culture supernatant Substances 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 230000002951 depilatory effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000002592 echocardiography Methods 0.000 description 2
- 230000007705 epithelial mesenchymal transition Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 230000002519 immonomodulatory effect Effects 0.000 description 2
- 230000008105 immune reaction Effects 0.000 description 2
- 230000028993 immune response Effects 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 230000003902 lesion Effects 0.000 description 2
- 210000003141 lower extremity Anatomy 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000002107 myocardial effect Effects 0.000 description 2
- 208000010125 myocardial infarction Diseases 0.000 description 2
- 230000002980 postoperative effect Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000007634 remodeling Methods 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 230000002269 spontaneous effect Effects 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 210000003437 trachea Anatomy 0.000 description 2
- 231100000582 ATP assay Toxicity 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 238000011740 C57BL/6 mouse Methods 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 208000013875 Heart injury Diseases 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 206010061216 Infarction Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 208000019693 Lung disease Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 230000009815 adipogenic differentiation Effects 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 239000003994 anesthetic gas Substances 0.000 description 1
- 230000002491 angiogenic effect Effects 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 206010003119 arrhythmia Diseases 0.000 description 1
- 230000006793 arrhythmia Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000002457 bidirectional effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 230000036770 blood supply Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 230000000747 cardiac effect Effects 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000009087 cell motility Effects 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 239000002458 cell surface marker Substances 0.000 description 1
- 238000002659 cell therapy Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 230000001447 compensatory effect Effects 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 210000004351 coronary vessel Anatomy 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 239000011557 critical solution Substances 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 230000003205 diastolic effect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000002934 diuretic Substances 0.000 description 1
- 229940030606 diuretics Drugs 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000001700 effect on tissue Effects 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 210000003722 extracellular fluid Anatomy 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 239000000834 fixative Substances 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 210000004704 glottis Anatomy 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000011702 heart failure animal model Methods 0.000 description 1
- 230000004217 heart function Effects 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 230000004957 immunoregulator effect Effects 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000007574 infarction Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229940124975 inotropic drug Drugs 0.000 description 1
- 102000006495 integrins Human genes 0.000 description 1
- 108010044426 integrins Proteins 0.000 description 1
- 230000003601 intercostal effect Effects 0.000 description 1
- 210000000876 intercostal muscle Anatomy 0.000 description 1
- 210000005248 left atrial appendage Anatomy 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000009818 osteogenic differentiation Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 206010033675 panniculitis Diseases 0.000 description 1
- 210000003540 papillary muscle Anatomy 0.000 description 1
- 210000003516 pericardium Anatomy 0.000 description 1
- 210000003800 pharynx Anatomy 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 201000003144 pneumothorax Diseases 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000009958 sewing Methods 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 210000004304 subcutaneous tissue Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 238000002627 tracheal intubation Methods 0.000 description 1
- 210000001364 upper extremity Anatomy 0.000 description 1
- 229940124549 vasodilator Drugs 0.000 description 1
- 239000003071 vasodilator agent Substances 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
- 210000002417 xiphoid bone Anatomy 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3834—Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0668—Mesenchymal stem cells from other natural sources
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2207/00—Modified animals
- A01K2207/30—Animals modified by surgical methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/105—Murine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/108—Swine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/03—Animal model, e.g. for test or diseases
- A01K2267/035—Animal model for multifactorial diseases
- A01K2267/0375—Animal model for cardiovascular diseases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/20—Materials or treatment for tissue regeneration for reconstruction of the heart, e.g. heart valves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/30—Synthetic polymers
- C12N2533/32—Polylysine, polyornithine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/52—Fibronectin; Laminin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2539/00—Supports and/or coatings for cell culture characterised by properties
- C12N2539/10—Coating allowing for selective detachment of cells, e.g. thermoreactive coating
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Public Health (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Epidemiology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Dermatology (AREA)
- Hematology (AREA)
- Pharmacology & Pharmacy (AREA)
- Microbiology (AREA)
- Rheumatology (AREA)
- General Chemical & Material Sciences (AREA)
- Heart & Thoracic Surgery (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Botany (AREA)
- Cardiology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Virology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Materials For Medical Uses (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
提供了一种治疗受试者中的心脏组织损伤或心功能不全相关的疾病的方法,该方法包括对受试者的心脏局部应用间充质干细胞膜片,例如脐带间充质干细胞膜片的步骤。还提供了间充质干细胞膜片的相关用途和组合物。
Description
PCT国内申请,说明书已公开。
Claims (20)
- PCT国内申请,权利要求书已公开。
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/CN2020/073765 WO2021146994A1 (zh) | 2020-01-22 | 2020-01-22 | 间充质干细胞膜片及其用途 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN113498434A true CN113498434A (zh) | 2021-10-12 |
Family
ID=76991962
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202080000065.XA Pending CN113498434A (zh) | 2020-01-22 | 2020-01-22 | 间充质干细胞膜片及其用途 |
CN202180003593.5A Pending CN113891933A (zh) | 2020-01-22 | 2021-01-22 | 间充质干细胞膜片及其用途 |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202180003593.5A Pending CN113891933A (zh) | 2020-01-22 | 2021-01-22 | 间充质干细胞膜片及其用途 |
Country Status (3)
Country | Link |
---|---|
US (1) | US20220347346A1 (zh) |
CN (2) | CN113498434A (zh) |
WO (2) | WO2021146994A1 (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114984052A (zh) * | 2022-07-25 | 2022-09-02 | 京东方科技集团股份有限公司 | 人源间充质干细胞膜片在子宫瘢痕治疗中的用途 |
CN115804869B (zh) * | 2022-10-26 | 2024-07-23 | 王丽 | 一种BADSCs膜片与导电纳米纤维复合心脏贴片及其制备方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104762257A (zh) * | 2015-04-13 | 2015-07-08 | 云南和泽西南生物科技有限公司 | 一种从脐带制备间充质干细胞的方法 |
CN109097326A (zh) * | 2018-08-10 | 2018-12-28 | 广东唯泰生物科技有限公司 | 一种制备间充质干细胞外泌体的方法及其应用 |
US20190274300A1 (en) * | 2016-11-25 | 2019-09-12 | Terumo Kabushiki Kaisha | Preservative solution for live cells or composition containing live cells |
CN110669727A (zh) * | 2019-11-07 | 2020-01-10 | 深圳科康干细胞技术有限公司 | 骨髓间充质干细胞膜片的制备方法及其应用 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2266499B1 (en) * | 2003-08-01 | 2015-06-10 | Cellseed Inc. | Three-dimensional tissue structure |
WO2006080434A1 (ja) * | 2005-01-27 | 2006-08-03 | Japan Health Sciences Foundation | 間葉系幹細胞を含む細胞シート |
US8568761B2 (en) * | 2005-07-15 | 2013-10-29 | Cormatrix Cardiovascular, Inc. | Compositions for regenerating defective or absent myocardium |
CN102628028A (zh) * | 2012-03-31 | 2012-08-08 | 北京大学第三医院 | 无载体骨髓间充质干细胞膜片的制备方法 |
AU2013271364B2 (en) * | 2012-06-07 | 2017-03-02 | The University Of Queensland | Release media |
EP3168297B1 (en) * | 2014-07-11 | 2020-09-16 | Metcela Inc. | Cardiac cell culture material |
CN111621474A (zh) * | 2019-02-28 | 2020-09-04 | 京东方科技集团股份有限公司 | 间充质干细胞膜片及其制备方法 |
CN111621475A (zh) * | 2019-02-28 | 2020-09-04 | 京东方科技集团股份有限公司 | 脐带间充质干细胞膜片及其制备方法 |
-
2020
- 2020-01-22 CN CN202080000065.XA patent/CN113498434A/zh active Pending
- 2020-01-22 WO PCT/CN2020/073765 patent/WO2021146994A1/zh active Application Filing
-
2021
- 2021-01-22 CN CN202180003593.5A patent/CN113891933A/zh active Pending
- 2021-01-22 WO PCT/CN2021/073295 patent/WO2021148000A1/zh active Application Filing
- 2021-01-22 US US17/430,086 patent/US20220347346A1/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104762257A (zh) * | 2015-04-13 | 2015-07-08 | 云南和泽西南生物科技有限公司 | 一种从脐带制备间充质干细胞的方法 |
US20190274300A1 (en) * | 2016-11-25 | 2019-09-12 | Terumo Kabushiki Kaisha | Preservative solution for live cells or composition containing live cells |
CN109097326A (zh) * | 2018-08-10 | 2018-12-28 | 广东唯泰生物科技有限公司 | 一种制备间充质干细胞外泌体的方法及其应用 |
CN110669727A (zh) * | 2019-11-07 | 2020-01-10 | 深圳科康干细胞技术有限公司 | 骨髓间充质干细胞膜片的制备方法及其应用 |
Non-Patent Citations (3)
Title |
---|
ISHIKANE S.等: "Allogenetic transplantation of fetal membrane-derived mesenchymal stem cell sheets increases neovascularization and improves cardiac function after myocardial infarction in rats", TRANSPLANTATION, vol. 96, no. 8, pages 697 - 706, XP055831110, DOI: 10.1097/TP.0b013e31829f753d * |
包杰文: "人尿源干细胞用于组织工程构建的可行性研究", 中国优秀硕士学位论文全文数据库 医药卫科技辑, no. 7, pages 11 * |
胡莉娟等: "《生物制药工艺》", 31 January 2016, 重庆大学出版社, pages: 239 * |
Also Published As
Publication number | Publication date |
---|---|
WO2021148000A1 (zh) | 2021-07-29 |
US20220347346A1 (en) | 2022-11-03 |
WO2021146994A1 (zh) | 2021-07-29 |
CN113891933A (zh) | 2022-01-04 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Tan et al. | Repair of infarcted myocardium using mesenchymal stem cell seeded small intestinal submucosa in rabbits | |
JP4709393B2 (ja) | 三次元的間質組織 | |
KR102565022B1 (ko) | 제대 중간엽 줄기 세포 및 그의 세포 시트의 제조 방법 | |
CN108525021A (zh) | 基于3d打印的含血管及毛囊结构组织工程皮肤及其制备方法 | |
US20090053277A1 (en) | Cell Sheet Containing Mesenchymal Stem Cells | |
US11890395B2 (en) | Three dimensional tissue compositions and methods of use | |
US7419661B2 (en) | Dermal sheath tissue in wound healing | |
CN111467575B (zh) | 一种集成有诱导多能干细胞来源的心肌细胞导电微针补片及其制备方法和应用 | |
JP2022506212A (ja) | 改変組織の凍結保存及び再構成のための組成物及び方法 | |
WO2021146994A1 (zh) | 间充质干细胞膜片及其用途 | |
Shudo et al. | Layered smooth muscle cell–endothelial progenitor cell sheets derived from the bone marrow augment postinfarction ventricular function | |
US7816133B2 (en) | Preparation of fibroblast-populated connective tissue substitute | |
CN112870445A (zh) | 一种软组织修复材料的制备方法及应用 | |
WO2018155651A1 (ja) | 線維芽細胞を含む心臓疾患を治療するための注射用組成物、及び治療用線維芽細胞の製造方法 | |
EP0980270B1 (en) | Dermal sheath tissue in wound healing | |
CN110201232B (zh) | 一种自组装预血管化干细胞膜片的制备方法 | |
Cui et al. | Effects of cartilage-derived morphogenetic protein 1 (CDMP1) transgenic mesenchymal stem cell sheets in repairing rabbit cartilage defects | |
Guo et al. | Engineering cardiac tissue from embryonic stem cells | |
US20240131225A1 (en) | Three dimensional tissue compositions and methods of use | |
US20230020862A1 (en) | Proangiogenic protein cocktails delivered in custom biomaterials to revascularize ischemic tissue | |
RU2268061C1 (ru) | Биотрансплантат, способ его получения (варианты) и способ лечения ишемической болезни сердца | |
RU2268062C1 (ru) | Биотрансплантат, способ его получения (варианты) и способ лечения дилятационной кардиомиопатии | |
RU2299073C1 (ru) | Биотрансплантат и способ лечения хронической сердечной недостаточности (варианты) | |
CN115216438A (zh) | 一种基于大蓝闪蝶翅膀的工程化心肌组织的制备方法及其应用 | |
CN117143807A (zh) | 一种血管类器官及其制备方法、细胞治疗组合物及其在缺血疾病中的应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination |