CN113462558B - High-density fermentation culture equipment and preparation process for veterinary traditional Chinese medicine microecological preparation - Google Patents

High-density fermentation culture equipment and preparation process for veterinary traditional Chinese medicine microecological preparation Download PDF

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CN113462558B
CN113462558B CN202111033726.1A CN202111033726A CN113462558B CN 113462558 B CN113462558 B CN 113462558B CN 202111033726 A CN202111033726 A CN 202111033726A CN 113462558 B CN113462558 B CN 113462558B
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cavity
traditional chinese
chinese medicine
tank
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CN113462558A (en
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孙新堂
李安东
朱瑞娟
朱术会
钱淼
王成森
杨文文
王传春
刘子铭
魏云飞
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Shandong Vocational Animal Science and Veterinary College
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Abstract

The invention provides high-density fermentation culture equipment and a preparation process for a veterinary traditional Chinese medicine microecological preparation, which comprise a fermentation tank and a detection control system, wherein a first fermentation cavity and a second fermentation cavity which are communicated with each other are arranged in the fermentation tank, a stirring mechanism and a defoaming mechanism are respectively arranged in the first fermentation cavity and the second fermentation cavity, the first fermentation cavity is connected with a material supplementing mechanism, a timing mechanism is arranged in the fermentation tank, the first fermentation cavity and the second fermentation cavity are respectively connected with a sampling mechanism and a vacuum pump, a heating cavity is arranged in the fermentation tank, the detection control system comprises a controller and a control panel, temperature sensors and pH detectors are respectively arranged in the first fermentation cavity and the second fermentation cavity, and the first fermentation cavity and the second fermentation cavity are respectively connected with an oxygen content dissolution detector. Reduces the risk of wasting a large amount of strains and Chinese medicinal materials.

Description

High-density fermentation culture equipment and preparation process for veterinary traditional Chinese medicine microecological preparation
Technical Field
The invention relates to the technical field of microecologics, in particular to high-density fermentation culture equipment and a preparation process of a traditional Chinese medicine microecologics for livestock.
Background
The traditional Chinese medicine and probiotic composite microecological preparation is used as a feed additive for replacing antibiotics due to the unique action and the advantages of no toxicity, no residue, no drug resistance and the like, so that the condition that the antibiotics are generally used as the feed additive in the breeding industry of China is changed, and the serious problems that the drug resistance brought by the antibiotics for feeding, the antibiotic residue in animal products threatens the health and safety of human beings and the like are solved.
The bacillus natto and the clostridium butyricum are important microecologics, the bacillus natto can be planted in intestinal tracts, the intestinal regulation effect is effectively exerted, the effects of enhancing immunity, resisting cancer and the like are achieved, the clostridium butyricum can promote the growth of beneficial bacteria and inhibit the reproduction of harmful bacteria after entering the intestinal tracts, the proliferation and the development of beneficial bacteria in the intestinal tracts are promoted, the disturbance of the bacteria in the intestinal tracts is corrected, and the occurrence of enterotoxin is reduced.
The existing fermentation culture device for the microecologics mainly has the following problems: the first is that the whole fermentation process can be completed by using two fermentation tanks, so that the conveying distance of the strain culture solution is relatively long, the strain culture solution is very easy to be infected by mixed bacteria in the conveying process, and the two fermentation tanks need to consume more energy to ferment, so that a large amount of energy is consumed for completing the whole fermentation process; secondly, in the fermentation process, an operator cannot sample and detect the fermentation liquor in the fermentation tank, can only sample and detect the fermentation liquor after the fermentation is finished, and can not remedy errors in the fermentation process, so that a great amount of waste of probiotics and Chinese medicinal materials is easily caused; thirdly, the time interval of feeding can not be controlled according to the stirring speed, and the problem of uneven mixing of feeding and fermentation liquor is very easy to occur, thus influencing the fermentation culture effect of the strains.
Therefore, a high-density fermentation culture device and a preparation process for the veterinary traditional Chinese medicine microecological preparation are needed.
Disclosure of Invention
The invention provides high-density fermentation culture equipment and a preparation process for a veterinary traditional Chinese medicine microecological preparation, which greatly reduce the probability of infection of a strain culture solution by mixed bacteria and energy consumed by fermentation, realize the function of sampling without stopping the machine, and reduce the risk of wasting a large amount of strains and traditional Chinese medicines.
One of the technical schemes of the invention is realized as follows:
the high-density fermentation culture equipment for the veterinary traditional Chinese medicine microecological preparation comprises a fermentation tank and a detection control system;
a first fermentation cavity and a second fermentation cavity which are communicated with each other are arranged in the fermentation tank, a stirring mechanism and a defoaming mechanism are arranged in each of the first fermentation cavity and the second fermentation cavity, the first fermentation cavity is connected with a material supplementing mechanism for supplementing materials, a timing mechanism for timing the interval time of the material supplementing is arranged in the fermentation tank, the first fermentation cavity and the second fermentation cavity are respectively connected with a sampling mechanism and a vacuum pump, a heating cavity for adjusting the fermentation temperature is arranged in the fermentation tank, and the heating cavity is surrounded and arranged on the outer sides of the first fermentation cavity and the second fermentation cavity;
detect control system including controller and the control panel that electric connection is in the same place, temperature sensor and pH detector are all installed to first fermentation chamber and second fermentation intracavity, first fermentation chamber and second fermentation chamber still are connected with a dissolved oxygen volume detector respectively, temperature sensor, pH detector and dissolved oxygen volume detector all with the controller electricity is connected.
As a preferred technical scheme, first fermentation chamber and second fermentation chamber all communicate there is a first inlet pipe, first fermentation chamber through communicating pipe with second fermentation chamber is linked together, install the diaphragm pump on communicating pipe, a check valve is installed respectively to the both ends of communicating pipe, the fluid-discharge tube is installed to the bottom in second fermentation chamber, install the flowing back valve on the flowing back tube, diaphragm pump, check valve and flowing back valve all with the controller electricity is connected.
As a preferable technical scheme, each stirring mechanism comprises a stirring shaft which extends along the axial direction of the fermentation tank, the two stirring shafts are respectively and rotatably arranged in the first fermentation cavity and the second fermentation cavity, the two stirring shafts are jointly connected with a speed reducing motor in a transmission way, gear motor with the controller electricity is connected, each equal interval is provided with a plurality of stirring vane on the lateral wall of (mixing) shaft, each the both ends of (mixing) shaft are equallyd divide and are do not fixed with a collar, are located same stir epaxial two be provided with a pair of axis of rotation between the collar, each the axis of rotation all with the (mixing) shaft parallels, each the both ends of axis of rotation are equallyd divide do not with correspond the collar swivelling joint is in the same place, each equal spiral shell screw installs a helical blade on the lateral wall of axis of rotation, it is every to rotate epaxial helical blade's direction of delivery is all opposite.
As an optimal technical scheme, each defoaming mechanism all includes a plurality of mounting panels, each the mounting panel is all installed in the correspondence the upper end of (mixing) shaft, each the mounting panel is all followed the axial vertical direction of (mixing) shaft extends the setting, each bottom on the mounting panel all is fixed with a plurality of broken teeth that are used for carrying out the breakage to the bubble, first fermentation chamber and second fermentation chamber communicate respectively has a second inlet pipe that is used for letting in the defoaming agent.
As a preferred technical scheme, the feed supplement mechanism including install in feed supplement pipe on the fermentation cylinder, rotary joint is installed to the discharge end of feed supplement pipe, rotary joint with be located the upper end of the (mixing) shaft in first fermentation chamber is installed together, the (mixing) shaft inside be equipped with the feed supplement chamber that the feed supplement pipe is linked together, install on the lateral wall of (mixing) shaft a plurality of with the blowing head that the feed supplement chamber is linked together, install a feed supplement valve on the feed supplement pipe, the feed supplement valve with the controller electricity is connected.
As an optimal technical scheme, a cavity is arranged inside the fermentation tank, the cavity is arranged above the first fermentation cavity and the second fermentation cavity, a driving gear is installed inside the cavity in a rotating mode, the driving gear is in transmission connection with a motor shaft of the speed reducing motor, the upper end of the stirring shaft extends into the cavity, the stirring shaft extends into one end of the cavity, a driven gear is fixedly installed at one end of the cavity, and the driven gear is meshed with the driving gear.
As a preferable technical scheme, the timing mechanism comprises a counter arranged in the cavity, the counter is arranged right above the first fermentation cavity, a counting shifting piece is arranged at the bottom of the counter, a counting rod is arranged on the end face corresponding to the driven gear, the counting rod is matched with the counting shifting piece, and the counter is electrically connected with the controller.
As a preferred technical scheme, each sampling mechanism comprises a sampling pipe, the liquid inlet ends of the two sampling pipes are respectively communicated with the first fermentation cavity and the second fermentation cavity, the liquid outlet end of each sampling pipe extends out of the fermentation tank, and a first communicating valve and a second communicating valve are arranged at intervals at one end of each sampling pipe extending out of the fermentation tank.
The other technical scheme of the invention is realized as follows:
the preparation process of the traditional Chinese medicine microecological preparation for livestock comprises the following steps:
s1, selecting 6 traditional Chinese medicines including astragalus, codonopsis pilosula, burdock, bighead atractylodes rhizome, angelica and ligusticum chuanxiong hort, respectively crushing the six traditional Chinese medicines by using different crushers, and screening the crushed six traditional Chinese medicines by using a 80-mesh sieve to obtain traditional Chinese medicine powder of the six traditional Chinese medicines;
s2, according to the weight parts of raw materials, the traditional Chinese medicine powder of the six traditional Chinese medicines is introduced into a medicine material mixing tank to be mixed by adopting the proportion of 10 parts of astragalus, 10 parts of codonopsis pilosula, 5 parts of burdock, 3 parts of bighead atractylodes rhizome, 3 parts of angelica and 3 parts of ligusticum chuanxiong hort to prepare a compound preparation;
s3, processing the compound preparation by adopting a decoction method to prepare a traditional Chinese medicine extracting solution, introducing the traditional Chinese medicine extracting solution into an extracting solution storage tank, and storing the traditional Chinese medicine extracting solution for later use at the temperature of 4 ℃;
s4, respectively inoculating the bacterial liquids of clostridium butyricum and bacillus natto in respective liquid culture media, respectively introducing the bacterial liquids of the two probiotics into first fermentation cavities of the two high-density fermentation culture devices for the veterinary traditional Chinese medicine microecological preparation for livestock, performing fermentation culture, and continuously supplementing materials through a material supplementing pipe in the fermentation process to obtain bacillus natto with the viable count not less than 5 multiplied by 109cfu/ml, and the number of viable bacteria of Clostridium butyricum is not less than 1 × 109cfu/ml of two strain culture solutions;
s5, introducing a strain culture solution in a first fermentation cavity of the high-density fermentation culture equipment for the veterinary traditional Chinese medicine microecological preparation into corresponding second fermentation cavities through a communicating pipe, simultaneously introducing appropriate amounts of traditional Chinese medicine extracting solutions into the two second fermentation cavities respectively according to the proportion of 40L of the strain culture solution to 100ml of the traditional Chinese medicine extracting solution, vacuumizing the second fermentation cavities by using a vacuum pump, and then performing anaerobic fermentation on the strain culture solution and the traditional Chinese medicine extracting solution in the second fermentation cavities to obtain fermentation liquor of the two probiotics;
s6, respectively discharging fermentation liquids of the two probiotics out of high-density fermentation culture equipment of the veterinary traditional Chinese medicine microecological preparation through liquid discharge pipes corresponding to the bottoms of the second fermentation cavities, and introducing the fermentation liquids of the two probiotics into a fermentation liquid mixing tank according to the ratio of 1:5 of the bacillus natto and the clostridium butyricum to mix and stir to obtain a fermentation mixture;
s7, introducing the fermented mixture into a spray drying tower for drying, and preparing the traditional Chinese medicine microecological preparation.
As a preferred technical solution, the step of processing the compound preparation by the decoction method in step S3 is:
s301, pouring the compound preparation and water into a cooking pot according to the proportion of 1g of the compound preparation and 10ml of water, and soaking the compound preparation for 0.5 h;
s302, boiling water by using a cooking pot, decocting for 0.5 hour by slow fire, and filtering to obtain filter residue and filtrate;
s303, carrying out secondary decoction and filtration on the filter residue by using a digester to obtain new filtrate;
s304, introducing the filtrates obtained in the steps S302 and S303 into an extracting solution mixing tank for mixing and concentrating to obtain a traditional Chinese medicine extracting solution with the concentration of 1g/ml, and introducing the traditional Chinese medicine extracting solution into an extracting solution storage tank.
By adopting the technical scheme, the invention has the beneficial effects that:
because the veterinary traditional Chinese medicine microecological preparation high-density fermentation culture equipment comprises a fermentation tank, wherein a first fermentation chamber, a second fermentation chamber and a heating chamber are arranged in the fermentation tank, in the invention, fermentation culture is carried out on bacillus natto liquid and clostridium butyricum liquid in the first fermentation chamber, strain culture solutions of two probiotics are respectively obtained, the strain culture solutions are introduced into the second fermentation chamber through a communicating pipe and are subjected to anaerobic fermentation after being mixed with a traditional Chinese medicine extracting solution, the whole fermentation process can be completed in the first fermentation chamber and the second fermentation chamber of the same fermentation tank, the conveying distance of the strain culture solution is shortened, and the risk and the probability of the strain culture solution being polluted by mixed bacteria in the conveying process are also greatly reduced; after the strain culture solution in the first fermentation cavity is introduced into the second fermentation cavity, the next batch of bacterial solution can be introduced into the first fermentation cavity for fermentation culture, the fermentation temperature ranges of the bacterial solution and the strain culture solution are approximately the same, the first fermentation cavity and the second fermentation cavity can be simultaneously heated by utilizing the heating cavity, the preparation efficiency of the traditional Chinese medicine microecological preparation is improved, and the energy consumed by fermentation is greatly reduced.
Because the first fermentation cavity and the second fermentation cavity are both provided with the stirring mechanisms, in the invention, the speed reduction motor drives the two stirring shafts to rotate through the power transmission of the driving gear and the driven gear, the stirring shafts drive the stirring blades and the rotating shafts to rotate around the stirring shafts so as to stir the strain culture solution in the first fermentation cavity and the fermentation liquid in the second fermentation cavity, the stirring blades stir and mix the strain culture solution and the fermentation liquid, the rotating shafts can rotate under the blocking action of the strain culture solution and the fermentation liquid in the rotating process around the stirring shafts, and stir and mix the strain culture solution and the fermentation liquid in the vertical direction, so that the full stirring and mixing of the strain culture solution in the first fermentation cavity and the fermentation liquid in the second fermentation cavity are realized.
Because first fermentation chamber and second fermentation intracavity all are provided with defoaming mechanism, in fermentation process, the foaming phenomenon can all appear in the zymotic fluid in the bacterial culture liquid of first fermentation intracavity and the second fermentation intracavity, let in the defoaming agent in first fermentation chamber and second fermentation intracavity through the second inlet pipe on the one hand, reduce the foaming volume in the fermentation process, on the other hand (mixing) shaft is at rotatory in-process, can drive mounting panel and broken tooth and rotate round the (mixing) shaft, broken tooth can be broken the foam at rotatory in-process, thereby avoided bacterial culture liquid and zymotic fluid to escape from the fermentation cylinder and the condition of microbiological contamination takes place.
As the first fermentation cavity is connected with the feeding mechanism, feeding materials are intermittently fed into the first fermentation cavity through the feeding mechanism, and the feeding materials comprise a carbon source (glucose/glycerol) and limiting amino acids (a mixture of tyrosine, methionine and leucine) relatively lacking in a basic culture medium, so that the number of viable bacteria in the first fermentation cavity is obviously increased.
Because the first fermentation cavity and the second fermentation cavity are both connected with a sampling mechanism, in the invention, taking sampling of the strain culture solution in the first fermentation cavity as an example, an operator firstly opens a first communicating valve on a sampling pipe, the strain culture solution in the first fermentation cavity enters the sampling pipe, after the first communicating valve is closed, the sample solution in the sampling pipe stays in a pipe section between the first communicating valve and the second communicating valve, then the operator can connect a sampling container at the liquid outlet end of the sampling pipe, open the second communicating valve to enable the sample solution in the sampling pipe to flow into the sampling container, sampling of the strain culture solution in the first fermentation cavity is completed, non-stop sampling of the strain culture solution and the fermentation liquid is realized, the operator can master the fermentation conditions in the first fermentation cavity and the second fermentation cavity at any time, and when the operator has errors in the fermentation in the first fermentation cavity and the second fermentation cavity, the problem that a large amount of strains and Chinese medicinal materials are wasted due to errors in fermentation is solved, and the risk that the strains and the Chinese medicinal materials are wasted is reduced.
According to the invention, an operator can set the cycle value of the counter according to the stirring speed, each time the driven gear rotates for one circle, the counting rod on the driven gear is in one-time contact with the counting shifting sheet, the counter counts for one time until the counting of the counter reaches the cycle value, the counter sends a feeding signal to the controller, the controller controls the feeding valve to be opened, the feeding material in the feeding tube is uniformly sprayed into the first fermentation cavity through the stirring shaft and the spraying head, the strain culture solution is fed, the feeding material is uniformly mixed with the strain culture solution, meanwhile, the counting of the counter returns to zero and the timing is restarted, the intermittent feeding material feeding is realized, and the fermentation culture effect of the strain culture solution in the first fermentation cavity is effectively improved.
Because the detection control system comprises a controller, a control panel, a temperature sensor, a pH detector and a dissolved oxygen detector, in the invention, an operator can control the start and stop of the stirring mechanism, the material supplementing mechanism, the timing mechanism and the defoaming mechanism, the transfer of a strain culture solution and other operations through the control panel, the operator can send a start instruction to the controller after pressing a start button on the control panel, and the two vacuum pumps simultaneously perform negative pressure air extraction on the interiors of the first fermentation cavity and the second fermentation cavity so as to ensure that the interiors of the first fermentation cavity and the second fermentation cavity reach the vacuum degree required by fermentation; the pH detector detects the pH values in the first fermentation cavity and the second fermentation cavity, and adjusts the pH values in the first fermentation cavity and the second fermentation cavity by automatically feeding ammonia water; the dissolved oxygen detector detects the dissolved oxygen of the liquid in the first fermentation cavity and the second fermentation cavity, and the oxygen requirement in the whole fermentation process is ensured; the temperature sensor monitors the temperature in the first fermentation cavity and the temperature in the second fermentation cavity in real time.
When the temperature of the first fermentation cavity and the second fermentation cavity is monitored to be higher than the preset highest fermentation temperature, the temperature sensor sends a cooling signal to the controller, the temperature of the gas introduced into the heating cavity is reduced, so that the temperature of the first fermentation cavity and the temperature of the second fermentation cavity are reduced to be within the preset fermentation temperature range, otherwise, when the temperature of the first fermentation cavity and the temperature of the second fermentation cavity are monitored to be lower than the preset lowest fermentation temperature, the temperature sensor sends a heating signal to the controller, and the temperature of the gas introduced into the heating cavity is increased, so that the temperature of the first fermentation cavity and the temperature of the second fermentation cavity are increased to be within the preset fermentation temperature range.
According to the invention, when a strain culture solution in the first fermentation cavity is introduced into the second fermentation cavity, an operator sends a strain culture solution transfer signal to the controller through the control panel, the controller controls the two one-way valves to be opened, the diaphragm pump starts, the diaphragm pump pumps all the strain culture solution in the first fermentation cavity into the second fermentation cavity, the transfer speed of the strain culture solution is accelerated, the one-way valve close to the first fermentation cavity enables the strain culture solution to only flow into the communicating pipe from the first fermentation cavity, and the one-way valve close to the second fermentation cavity enables the strain culture solution to only flow into the second fermentation cavity from the communicating pipe, so that the backflow of the strain culture solution is avoided.
Because the preparation process of the veterinary traditional Chinese medicine microecological preparation adopts a decoction method to extract the traditional Chinese medicine, in the invention, the compound preparation prepared from the traditional Chinese medicine powder is decocted and filtered twice, so that the complete extraction of the effective components in the traditional Chinese medicine is realized, and the waste of the traditional Chinese medicine is avoided.
Because the preparation process of the veterinary traditional Chinese medicine microecological preparation adopts a spray drying tower to dry the fermentation liquor, in the invention, auxiliary materials are required to be added as a protective agent to reduce the damage of high temperature to thalli during drying, thereby ensuring the quality of the traditional Chinese medicine microecological preparation.
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In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic structural diagram according to a first embodiment of the present invention;
fig. 2 is a schematic connection diagram of a detection control system in an embodiment of the present invention;
FIG. 3 is an enlarged view of the structure at A in FIG. 1;
FIG. 4 is a flow chart of a second embodiment of the present invention.
Wherein: 1. a fermentation tank; 2. a first fermentation chamber; 3. a second fermentation chamber; 4. a vacuum pump; 5. a heating cavity; 6. a communicating pipe; 7. a controller; 8. a control panel; 9. a temperature sensor; 10. a pH detector; 11. a dissolved oxygen detector; 12. a first feed tube; 13. a diaphragm pump; 14. a one-way valve; 15. a liquid discharge pipe; 16. a drain valve; 17. a stirring shaft; 18. a reduction motor; 19. a stirring blade; 20. a mounting ring; 21. a rotating shaft; 22. a helical blade; 23. mounting a plate; 24. crushing teeth; 25. a second feed tube; 26. a material supplementing pipe; 27. a rotary joint; 28. a feeding cavity; 29. a material spraying head; 30. a make-up valve; 31. a cavity; 32. a drive gear; 33. a driven gear; 34. a counter; 35. counting the poking sheets; 36. a counting rod; 37. a sampling tube; 38. a first communication valve; 39. a second communicating valve; 40. a astragalus grinder; 41. mixing the medicinal materials; 42. an extract storage tank; 43. a fermentation liquor mixing tank; 44. a spray drying tower; 45. a cooking pan; 46. an extracting solution mixing tank; 47. a radix codonopsitis pulverizer; 48. a burdock grinder; 49. a white atractylodes rhizome pulverizer; 50. a Chinese angelica grinder; 51. ligusticum wallichii crusher.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example one
As shown in the figures 1-3, the high-density fermentation culture equipment for the veterinary Chinese medicine microecological preparation comprises a fermentation tank 1 and a detection control system.
Fermentation cylinder 1 is inside to be equipped with first fermentation chamber 2 and the second fermentation chamber 3 of intercommunication each other, all be provided with rabbling mechanism and defoaming mechanism in first fermentation chamber 2 and the second fermentation chamber 3, first fermentation chamber 2 is connected with and is used for carrying out the feed supplement mechanism of feed supplement to first fermentation chamber 2, be provided with in the fermentation cylinder 1 and be used for carrying out the timing mechanism of timing to feed supplement interval, first fermentation chamber 2 and second fermentation chamber 3 all are connected with a sampling mechanism and a vacuum pump 4, the inside heating chamber 5 that is used for adjusting the fermentation temperature that is equipped with of fermentation cylinder 1, heating chamber 5 surrounds and sets up in the outside of first fermentation chamber 2 and second fermentation chamber 3, in this embodiment, heat first fermentation chamber 2 and second fermentation chamber 3 through letting in high temperature gas in heating chamber 5.
In the invention, the bacterium liquid of the probiotics is fermented and cultured in the first fermentation cavity 2 to obtain the strain culture liquid, the strain culture liquid is introduced into the second fermentation cavity 3 through the communicating pipe 6 and is subjected to anaerobic fermentation after being mixed with the traditional Chinese medicine extracting solution, the whole fermentation process is completed in the first fermentation cavity 2 and the second fermentation cavity 3 of the same fermentation tank 1, the risk that the strain culture liquid is infected by mixed bacteria in the conveying process is effectively reduced, the first fermentation cavity 2 and the second fermentation cavity 3 can be fermented simultaneously, and the first fermentation cavity 2 and the second fermentation cavity 3 are heated simultaneously by using the heating cavity 5, so that the preparation efficiency of the traditional Chinese medicine microecological preparation is improved, and the energy consumed by fermentation is greatly reduced.
The detection control system comprises a controller 7 and a control panel 8 which are electrically connected together, wherein a temperature sensor 9 and a pH detector 10 are respectively installed in a first fermentation cavity 2 and a second fermentation cavity 3, the first fermentation cavity 2 and the second fermentation cavity 3 are respectively connected with a dissolved oxygen detector 11, the temperature sensor 9, the pH detector 10 and the dissolved oxygen detector 11 are electrically connected with the controller 7, in the embodiment, the controller 7 adopts a single chip microcomputer of CY7C1354C-250AXC type, the temperature sensor 9 adopts a temperature sensor of TR-02129 type, the pH detector 10 adopts a pH detector of SIN-PH160 type, and the dissolved oxygen detector 11 adopts a fluorescence dissolved oxygen instrument of MIK-DY2900 type controlled by Hangzhou American.
In the invention, taking the first fermentation chamber 2 as an example, the vacuum pump 4 is used for performing negative pressure air exhaust on the interior of the first fermentation chamber 2 so as to enable the interior of the first fermentation chamber 2 to reach the vacuum degree required by fermentation; the pH detector 10 is used for detecting the pH value of the strain culture solution in the first fermentation chamber 2, and adjusting the pH value of the strain culture solution by automatically feeding ammonia water to ensure that the pH value of the strain culture solution is in a preset pH value range; the dissolved oxygen detector 11 is used for detecting the dissolved oxygen of the strain culture solution in the first fermentation chamber 2, and ensuring that the content of the dissolved oxygen in the strain culture solution is enough to meet the requirement of the whole fermentation process; the temperature sensor 9 is used for monitoring the temperature in the first fermentation cavity 2 in real time, so that the temperature in the first fermentation cavity 2 is always kept in a preset fermentation temperature range.
The first fermentation chamber 2 and the second fermentation chamber 3 are both communicated with a first feeding pipe 12, the first fermentation chamber 2 is communicated with the second fermentation chamber 3 through a communicating pipe 6, a diaphragm pump 13 is installed on the communicating pipe 6, two ends of the communicating pipe 6 are respectively provided with a one-way valve 14, the bottom of the second fermentation chamber 3 is provided with a liquid discharge pipe 15, the liquid discharge pipe 15 is provided with a liquid discharge valve 16, and the diaphragm pump 13, the one-way valve 14 and the liquid discharge valve 16 are all electrically connected with a controller 7.
Wherein each stirring mechanism comprises a stirring shaft 17 which extends along the axial direction of the fermentation tank 1, the two stirring shafts 17 are respectively and rotatably arranged in the first fermentation chamber 2 and the second fermentation chamber 3, the two stirring shafts 17 are jointly connected with a speed reducing motor 18 in a transmission way, the speed reducing motor 18 is electrically connected with the controller 7, a plurality of stirring blades 19 are respectively arranged on the side wall of each stirring shaft 17 at intervals, two mounting rings 20 are respectively fixed at two ends of each stirring shaft 17, a pair of rotating shafts 21 are arranged between the two mounting rings 20 positioned on the same stirring shaft 17, each rotating shaft 21 is parallel to the stirring shaft 17, two ends of each rotating shaft 21 are respectively and rotatably connected with the corresponding mounting rings 20, a helical blade 22 is spirally arranged on the side wall of each rotating shaft 21, and the conveying directions of the helical blades 22 on each pair of rotating shafts 21 are opposite, stirring vane 19 carries out stirring and mixing in the horizontal direction to bacterial culture solution and zymotic fluid, and helical blade 22 carries out stirring in the vertical direction with bacterial culture solution and zymotic fluid and mixes, has realized fully stirring the mixture to the zymotic fluid in first fermentation chamber 2, second fermentation chamber 3.
Each defoaming mechanism comprises a plurality of mounting plates 23, each mounting plate 23 is mounted at the upper end of the corresponding stirring shaft 17, each mounting plate 23 extends along the axial vertical direction of the stirring shaft 17, a plurality of crushing teeth 24 for crushing bubbles are fixed at the bottom of each mounting plate 23, the first fermentation chamber 2 and the second fermentation chamber 3 are respectively communicated with a second feeding pipe 25 for introducing a defoaming agent, in the invention, the defoaming agent is introduced into the first fermentation chamber 2 and the second fermentation chamber 3 through the second feeding pipe 25 to reduce the foaming amount in the fermentation process, and the crushing teeth 24 on the stirring shaft 17 crush the bubbles, so that the condition that strain culture solution and fermentation broth escape from the fermentation tank 1 to contaminate bacteria is avoided, in the embodiment, the defoaming agent can be organic silicon.
In addition, the material supplementing mechanism comprises a material supplementing pipe 26 arranged on the fermentation tank 1, a rotary joint 27 is arranged at the discharge end of the material supplementing pipe 26, the rotary joint 27 is arranged together with the upper end of a stirring shaft 17 positioned in the first fermentation cavity 2, a material supplementing cavity 28 communicated with the material supplementing pipe 26 is arranged inside the stirring shaft 17, a plurality of material spraying heads 29 communicated with the material supplementing cavity 28 are arranged on the side wall of the stirring shaft 17, a material supplementing valve 30 is arranged on the material supplementing pipe 26, and the material supplementing valve 30 is electrically connected with the controller 7.
The fermentation tank 1 is internally provided with a cavity 31, the cavity 31 is arranged above the first fermentation chamber 2 and the second fermentation chamber 3, a driving gear 32 is rotatably arranged inside the cavity 31, the driving gear 32 is connected with a motor shaft of the speed reducing motor 18 in a transmission way, the upper end of each stirring shaft 17 extends into the cavity 31, one end of each stirring shaft 17 extending into the cavity 31 is fixedly provided with a driven gear 33, and each driven gear 33 is meshed with the driving gear 32.
The timing mechanism comprises a counter 34 arranged in the cavity 31, the counter 34 is arranged right above the first fermentation cavity 2, a counting poking sheet 35 is arranged at the bottom of the counter 34, a counting rod 36 is arranged on the end surface corresponding to the driven gear 33, the counting rod 36 is matched with the counting poking sheet 35, the counter 34 is electrically connected with the controller 7, in the invention, the counting rod 36 on the driven gear 33 is contacted with the counting poking sheet 35 once when the driven gear 33 rotates for one circle, the counter 34 counts for one until the counting of the counter 34 reaches a cycle value, the counter 34 sends a signal to the controller 7, the controller 7 controls the feeding valve 30 to open to feed the first fermentation cavity 2, meanwhile, the counter 34 counts for zero and starts timing again, the counter 34 controls the feeding interval time according to the stirring rotating speed, and realizes intermittent feeding, effectively improves the fermentation culture effect of the strain culture solution in the first fermentation cavity 2.
In addition, each sampling mechanism comprises a sampling pipe 37, the liquid inlet ends of the two sampling pipes 37 are respectively communicated with the first fermentation cavity 2 and the second fermentation cavity 3, the liquid outlet end of each sampling pipe 37 extends out of the fermentation tank 1, and one end of each sampling pipe 37 extending out of the fermentation tank 1 is provided with a first communicating valve 38 and a second communicating valve 39 at intervals.
Example two
As shown in fig. 1 and fig. 4, the preparation process of the veterinary Chinese medicinal microecological preparation comprises the following steps:
s1, selecting 6 traditional Chinese medicines including astragalus mongholicus, codonopsis pilosula, burdock, bighead atractylodes rhizome, angelica sinensis and ligusticum chuanxiong hort, respectively crushing the six traditional Chinese medicines by using a astragalus mongholicus crusher 40, a codonopsis pilosula crusher 47, a burdock crusher 48, a bighead atractylodes rhizome crusher 49, an angelica sinensis crusher 50 and a ligusticum chuanxiong hort crusher 51, and screening the crushed six traditional Chinese medicines by using a 80-mesh sieve to obtain traditional Chinese medicine powder of the six traditional Chinese medicines.
S2, according to the weight parts of raw materials, the traditional Chinese medicine powder of the six traditional Chinese medicines is introduced into a same medicine mixing tank 41 to be mixed by adopting the proportion of 10 parts of astragalus, 10 parts of codonopsis pilosula, 5 parts of burdock, 3 parts of bighead atractylodes rhizome, 3 parts of angelica and 3 parts of ligusticum chuanxiong hort, so as to prepare the compound preparation.
S3, processing the compound preparation by decoction to obtain Chinese medicinal extractive solution, introducing the Chinese medicinal extractive solution into the extractive solution storage tank 42, and storing at 4 deg.C.
S4, respectively inoculating the bacteria liquid of clostridium butyricum and bacillus natto in respective liquid culture medium, respectively introducing the bacteria liquid of the two probiotics into the first fermentation chamber 2 of the fermentation tanks 1 of the two veterinary traditional Chinese medicine microecological preparation high-density fermentation culture equipment, namely introducing clostridium butyricum liquid into the first fermentation chamber 2 in one fermentation tank 1, performing fermentation culture of the clostridium butyricum liquid in the first fermentation chamber 2, introducing bacillus natto liquid into the first fermentation chamber 2 in the other fermentation tank 1, performing fermentation culture of the bacillus natto liquid in the first fermentation chamber 2, and continuously supplementing materials into the two first fermentation chambers 2 through a material supplementing pipe 26 in the process of performing fermentation culture of the two probiotics to obtain the bacillus natto with the viable count not less than 5 multiplied by 109cfu/ml, and the number of viable bacteria of Clostridium butyricum is not less than 1 × 109cfu/ml of two kinds of bacteria culture solution, in this example, the inoculation amount of the bacteria solution is 2%, and the fermentation conditions of the bacteria solution are: the fermentation temperature is 30-37 ℃, the pH value is 6.0-7.5, the stirring speed is 100-.
S5, respectively introducing a strain culture solution in a first fermentation chamber 2 of high-density fermentation culture equipment for veterinary traditional Chinese medicine microecologics into corresponding second fermentation chambers 3 through communicating pipes 6, namely introducing the strain culture solution of clostridium butyricum in the first fermentation chamber 2 of a fermentation tank 1 for fermentation culture of clostridium butyricum into the second fermentation chamber 3 of the fermentation tank 1 through the communicating pipes 6, respectively introducing the strain culture solution of bacillus natto in the first fermentation chamber 2 of the fermentation tank 1 for fermentation culture of bacillus natto into the second fermentation chamber 3 of the fermentation tank 1 through the communicating pipes 6, respectively introducing proper amounts of traditional Chinese medicine extracting solutions into the second fermentation chambers 3 of the two fermentation tanks 1 according to the proportion of 40L of the strain culture solution and 100ml of the traditional Chinese medicine extracting solution, vacuumizing the second fermentation chambers 3 by using a vacuum pump 4, and then carrying out anaerobic fermentation on the strain culture solution and the traditional Chinese medicine extracting solution in the second fermentation chambers 3, the fermentation liquid of the two probiotics is obtained, in this example, the fermentation conditions of the strain culture liquid are as follows: the fermentation temperature is 30-37 ℃, the pH value is 6.0-7.5, the stirring speed is 100-.
S6, respectively discharging fermentation liquor of the two probiotics out of corresponding high-density fermentation culture equipment for the veterinary traditional Chinese medicine microecologics through a liquid discharge pipe 15, namely discharging the fermentation liquor of clostridium butyricum out of a second fermentation cavity 3 of a corresponding fermentation tank 1 through the liquid discharge pipe 15, discharging the fermentation liquor of bacillus natto out of the second fermentation cavity 3 of the corresponding fermentation tank 1 through the liquid discharge pipe 15, and introducing the fermentation liquor of the two probiotics into a fermentation liquor mixing tank 43 according to the ratio of bacillus natto to clostridium butyricum in a ratio of 1:5 for mixing and stirring to obtain a fermentation mixture.
S7, introducing the fermented mixture into a spray drying tower 44 for drying to prepare the traditional Chinese medicine microecological preparation, wherein in the invention, in order to reduce the damage of high temperature to thalli during drying and ensure the quality of the traditional Chinese medicine microecological preparation, auxiliary materials are required to be added as protective agents during drying, and the auxiliary materials can adopt low sugar, glycerol or a mixture of low sugar and glycerol.
Wherein, the step of processing the compound preparation by the decoction method in the step S3 comprises the following steps:
s301, pouring the compound preparation and water into a cooking pot 45 according to the proportion of 1g of the compound preparation and 10ml of water, and soaking the compound preparation for 0.5 h;
s302, boiling water by using a cooking pot 45, decocting for 0.5 hour by slow fire, and filtering to obtain filter residue and filtrate;
s303, carrying out secondary decoction and filtration on the filter residue by using the cooking pot 45 to obtain new filtrate;
s304, introducing the filtrate obtained in the steps S302 and S303 into an extracting solution mixing tank 46 for mixing and concentrating to obtain a traditional Chinese medicine extracting solution with the concentration of 1g/ml, and introducing the traditional Chinese medicine extracting solution into an extracting solution storage tank 42.
According to the invention, the microecological preparation is prepared by adopting two probiotics of bacillus natto and clostridium butyricum, 6 traditional Chinese medicines of astragalus, codonopsis pilosula, burdock, bighead atractylodes rhizome, angelica and ligusticum chuanxiong hort are used as fermentation substrates, and the 6 traditional Chinese medicines are prepared into the compound preparation through reasonable proportioning, so that the fermentation and proliferation effects of the probiotics can be effectively improved, and meanwhile, the bacillus natto and the clostridium butyricum can enhance the drug effect of the traditional Chinese medicines in animal bodies to a great extent; meanwhile, the bacillus natto and the clostridium butyricum have good synergistic effect, after the bacillus natto and the clostridium butyricum are scientifically compounded, the bacillus natto consumes oxygen in animal intestinal tracts, growth of the clostridium butyricum is facilitated, and the clostridium butyricum can generate butyric acid to play a role in the intestinal tracts.
In conclusion, the high-density fermentation culture equipment and the preparation process for the veterinary traditional Chinese medicine microecological preparation greatly reduce the probability of the strain culture solution infected by the mixed bacteria and the energy consumed by fermentation, simultaneously realize the function of sampling without stopping the machine, and reduce the risk of wasting a large amount of strains and traditional Chinese medicines.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (7)

1. The high-density fermentation culture equipment for the veterinary traditional Chinese medicine microecological preparation is characterized by comprising a fermentation tank and a detection control system;
a first fermentation cavity and a second fermentation cavity which are communicated with each other are arranged in the fermentation tank, a stirring mechanism and a defoaming mechanism are arranged in each of the first fermentation cavity and the second fermentation cavity, the first fermentation cavity is connected with a material supplementing mechanism for supplementing materials, a timing mechanism for timing the interval time of the material supplementing is arranged in the fermentation tank, the first fermentation cavity and the second fermentation cavity are respectively connected with a sampling mechanism and a vacuum pump, a heating cavity for adjusting the fermentation temperature is arranged in the fermentation tank, and the heating cavity is surrounded and arranged on the outer sides of the first fermentation cavity and the second fermentation cavity;
the detection control system comprises a controller and a control panel which are electrically connected together, wherein a temperature sensor and a pH detector are respectively arranged in the first fermentation cavity and the second fermentation cavity, the first fermentation cavity and the second fermentation cavity are respectively connected with a dissolved oxygen detector, and the temperature sensor, the pH detector and the dissolved oxygen detector are electrically connected with the controller;
each stirring mechanism comprises a stirring shaft which extends along the axial direction of the fermentation tank, the two stirring shafts are respectively and rotatably arranged in the first fermentation cavity and the second fermentation cavity, the two stirring shafts are jointly connected with a speed reducing motor in a transmission way, the speed reducing motor is electrically connected with the controller, a plurality of stirring blades are arranged on the side wall of each stirring shaft at intervals, two ends of each stirring shaft are respectively fixed with a mounting ring, a pair of rotating shafts are arranged between the two mounting rings on the same stirring shaft, each rotating shaft is parallel to the stirring shaft, two ends of each rotating shaft are respectively and rotatably connected with the corresponding mounting rings, a spiral blade is spirally arranged on the side wall of each rotating shaft, and the conveying direction of the spiral blade on each pair of rotating shafts is opposite;
the material supplementing mechanism comprises a material supplementing pipe arranged on the fermentation tank, a rotary joint is arranged at the discharge end of the material supplementing pipe, the rotary joint is arranged together with the upper end of a stirring shaft positioned in the first fermentation cavity, a material supplementing cavity communicated with the material supplementing pipe is arranged inside the stirring shaft, a plurality of material spraying heads communicated with the material supplementing cavity are arranged on the side wall of the stirring shaft, a material supplementing valve is arranged on the material supplementing pipe, and the material supplementing valve is electrically connected with the controller;
the timing mechanism comprises a counter arranged in the cavity, the counter is arranged right above the first fermentation cavity, a counting shifting piece is arranged at the bottom of the counter, a counting rod is arranged on the end surface corresponding to the driven gear, the counting rod is matched with the counting shifting piece, and the counter is electrically connected with the controller;
respectively inoculating the bacterial liquids of clostridium butyricum and bacillus natto in respective liquid culture media, respectively introducing the bacterial liquids of the two probiotics into two first fermentation cavities for fermentation culture, and continuously supplementing materials through a material supplementing pipe in the fermentation process to respectively obtain the bacillus natto with the viable count not less than 5 multiplied by 109cfu/ml Bacillus natto strain culture solution and Clostridium butyricum viable count of not less than 1 × 109cfu/ml of a strain culture solution of clostridium butyricum; the strain culture solution in a first fermentation cavity in the high-density fermentation culture equipment for the veterinary traditional Chinese medicine microecological preparation is introduced into corresponding second fermentation cavities through a communicating pipe, meanwhile, according to the proportion of 40L of the strain culture solution and 100ml of traditional Chinese medicine extracting solution, proper amount of traditional Chinese medicine extracting solution is introduced into the two second fermentation cavities respectively, and after the second fermentation cavities are vacuumized by a vacuum pump, the strain culture solution and the traditional Chinese medicine extracting solution are subjected to anaerobic fermentation in the second fermentation cavities to obtain fermentation liquor of the two probiotics;
respectively discharging fermentation liquor of the two probiotics out of high-density fermentation culture equipment of the veterinary traditional Chinese medicine microecological preparation through a liquid discharge pipe corresponding to the bottom of the second fermentation cavity, and introducing the fermentation liquor of the two probiotics into a fermentation liquor mixing tank according to the ratio of 1:5 of bacillus natto and clostridium butyricum to mix and stir to obtain a fermentation mixture;
and (3) introducing the fermentation mixture into a spray drying tower for drying to prepare the traditional Chinese medicine microecological preparation.
2. The high-density fermentation culture equipment for the microecological preparation of the traditional Chinese medicine for the veterinary use according to claim 1, wherein the first fermentation chamber and the second fermentation chamber are both communicated with a first feeding pipe, the first fermentation chamber is communicated with the second fermentation chamber through a communicating pipe, a diaphragm pump is installed on the communicating pipe, a one-way valve is respectively installed at two ends of the communicating pipe, a liquid discharge pipe is installed at the bottom of the second fermentation chamber, a liquid discharge valve is installed on the liquid discharge pipe, and the diaphragm pump, the one-way valve and the liquid discharge valve are all electrically connected with the controller.
3. The apparatus according to claim 1, wherein each defoaming mechanism comprises a plurality of mounting plates, each mounting plate is mounted at the upper end of the corresponding stirring shaft, each mounting plate extends along the axial direction of the stirring shaft, a plurality of crushing teeth for crushing bubbles are fixed at the bottom of each mounting plate, and the first fermentation chamber and the second fermentation chamber are respectively communicated with a second feeding pipe for introducing a defoaming agent.
4. The high-density fermentation culture equipment for the veterinary traditional Chinese medicine microecological preparation according to claim 1, wherein a cavity is arranged inside the fermentation tank, the cavity is arranged above the first fermentation chamber and the second fermentation chamber, a driving gear is rotatably arranged inside the cavity, the driving gear is in transmission connection with a motor shaft of the speed reduction motor, the upper end of each stirring shaft extends into the cavity, one end of each stirring shaft extending into the cavity is fixedly provided with a driven gear, and each driven gear is meshed with the driving gear.
5. The apparatus of claim 1, wherein each sampling mechanism comprises a sampling tube, the liquid inlet ends of the sampling tubes are respectively connected to the first fermentation chamber and the second fermentation chamber, the liquid outlet end of each sampling tube extends out of the fermentation tank, and the end of each sampling tube extending out of the fermentation tank is provided with a first communicating valve and a second communicating valve at intervals.
6. The preparation process of the veterinary traditional Chinese medicine microecological preparation is characterized by comprising the following steps of:
s1, selecting 6 traditional Chinese medicines including astragalus, codonopsis pilosula, burdock, bighead atractylodes rhizome, angelica and ligusticum chuanxiong hort, respectively crushing the six traditional Chinese medicines by using different crushers, and screening the crushed six traditional Chinese medicines by using a 80-mesh sieve to obtain traditional Chinese medicine powder of the six traditional Chinese medicines;
s2, according to the weight parts of raw materials, the traditional Chinese medicine powder of the six traditional Chinese medicines is introduced into a medicine material mixing tank to be mixed by adopting the proportion of 10 parts of astragalus, 10 parts of codonopsis pilosula, 5 parts of burdock, 3 parts of bighead atractylodes rhizome, 3 parts of angelica and 3 parts of ligusticum chuanxiong hort to prepare a compound preparation;
s3, processing the compound preparation by adopting a decoction method to prepare a traditional Chinese medicine extracting solution, introducing the traditional Chinese medicine extracting solution into an extracting solution storage tank, and storing the traditional Chinese medicine extracting solution for later use at the temperature of 4 ℃;
s4, respectively inoculating the bacterial liquids of clostridium butyricum and bacillus natto into respective liquid culture media, respectively introducing the bacterial liquids of the two probiotics into first fermentation cavities of two high-density fermentation culture devices of the veterinary traditional Chinese medicine microecological preparation for veterinary use as claimed in claim 1, performing fermentation culture, continuously supplementing materials through a material supplementing pipe in the fermentation process, and respectively obtaining the bacillus natto with the viable count not less than 5 x 109cfu/ml Bacillus natto strain culture solution and Clostridium butyricum viable count of not less than 1 × 109cfu/ml of a strain culture solution of clostridium butyricum;
s5, introducing a strain culture solution in a first fermentation cavity of the high-density fermentation culture equipment for the veterinary traditional Chinese medicine microecological preparation into corresponding second fermentation cavities through a communicating pipe, simultaneously introducing appropriate amounts of traditional Chinese medicine extracting solutions into the two second fermentation cavities respectively according to the proportion of 40L of the strain culture solution to 100ml of the traditional Chinese medicine extracting solution, vacuumizing the second fermentation cavities by using a vacuum pump, and then performing anaerobic fermentation on the strain culture solution and the traditional Chinese medicine extracting solution in the second fermentation cavities to obtain fermentation liquor of the two probiotics;
s6, respectively discharging fermentation liquids of the two probiotics out of high-density fermentation culture equipment of the veterinary traditional Chinese medicine microecological preparation through liquid discharge pipes corresponding to the bottoms of the second fermentation cavities, and introducing the fermentation liquids of the two probiotics into a fermentation liquid mixing tank according to the ratio of 1:5 of the bacillus natto and the clostridium butyricum to mix and stir to obtain a fermentation mixture;
s7, introducing the fermented mixture into a spray drying tower for drying, and preparing the traditional Chinese medicine microecological preparation.
7. The preparation process of the veterinary Chinese medicinal microecological preparation according to claim 6, wherein the step of processing the compound preparation by a decoction method in step S3 comprises the steps of:
s301, pouring the compound preparation and water into a cooking pot according to the proportion of 1g of the compound preparation and 10ml of water, and soaking the compound preparation for 0.5 h;
s302, boiling water by using a cooking pot, decocting for 0.5 hour by slow fire, and filtering to obtain filter residue and filtrate;
s303, carrying out secondary decoction and filtration on the filter residue by using a digester to obtain new filtrate;
s304, introducing the filtrates obtained in the steps S302 and S303 into an extracting solution mixing tank for mixing and concentrating to obtain a traditional Chinese medicine extracting solution with the concentration of 1g/ml, and introducing the traditional Chinese medicine extracting solution into an extracting solution storage tank.
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