CN112251338B - Enzyme preparation microbial strain liquid fermentation device and fermentation process thereof - Google Patents

Enzyme preparation microbial strain liquid fermentation device and fermentation process thereof Download PDF

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CN112251338B
CN112251338B CN202011521870.5A CN202011521870A CN112251338B CN 112251338 B CN112251338 B CN 112251338B CN 202011521870 A CN202011521870 A CN 202011521870A CN 112251338 B CN112251338 B CN 112251338B
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fermentation
fermentation tank
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pipe
liquid
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CN112251338A (en
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卞亦冰
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Tianjin Zhengxin HAOSI Technology Co.,Ltd.
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Tianjin Zhengxin Haosi Technology Co ltd
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Abstract

The invention provides an enzyme preparation microbial strain liquid fermentation device and a fermentation process thereof, wherein a horizontal screen is fixedly arranged at a liquid level line above the inside of a fermentation tank, a defoaming area is arranged above the horizontal screen, and a stirring area is arranged below the horizontal screen; a vertical screen is fixedly connected to the side wall of the rotating shaft in the upper defoaming area, and the lower end of the vertical screen is attached to the upper surface of the horizontal screen; a cooling coil is sleeved on the periphery of the rotating shaft positioned in the lower stirring area, and the water inlet end and the water outlet end of the cooling coil penetrate through the wall of the fermentation tank; fixedly connected with stirring piece and puddler in the pivot, the scraper blade is laminated mutually with the fermentation cylinder inboard wall. The fermentation process carried out by the device solves the problem that the fermentation effect is influenced by insufficient stirring and uneven distribution of dissolved oxygen in the fermentation process, and simultaneously the defoaming can be carried out under the condition of not influencing the dissolved oxygen.

Description

Enzyme preparation microbial strain liquid fermentation device and fermentation process thereof
Technical Field
The invention relates to the technical field of biological fermentation, in particular to an enzyme preparation microbial liquid fermentation device and a fermentation process thereof.
Background
Two fermentation production modes of the enzyme are provided, one is solid fermentation, and the other is liquid submerged fermentation. Solid fermentation processes are mainly used for the production of commercial enzymes of fungal origin, where the production of amylase from Aspergillus oryzae and the production of protease from Aspergillus and Mucor has a long history in China and Japan. Although this culture method is simple, the operating conditions are not easily controlled. Most enzymes are now produced by submerged liquid fermentation cultures.
Submerged fermentation, also known as submerged culture, utilizes a liquid medium in a fermentation tank with agitation and aeration. During the fermentation process, the strain can only utilize dissolved oxygen, so that a large amount of oxygen must be continuously supplemented into the fermentation liquor, and stirring is continuously carried out to improve the solubility of the oxygen in the fermentation liquor. The stirring mode of the existing fermentation tank is simple, and fermentation liquor can not be stirred sufficiently, so that local dissolved oxygen of the fermentation liquor is insufficient, and the fermentation effect is influenced. In addition, during fermentation, aeration, agitation, metabolic processes of microorganisms, decomposition of certain components in the medium, and the like may generate foam, too much permanent foam is disadvantageous to the fermentation, and the addition of antifoaming agents affects the dissolved oxygen content.
Disclosure of Invention
In view of the above, the present invention is directed to provide an enzyme preparation microbial liquid fermentation apparatus and a fermentation process thereof, so as to solve the problems of insufficient stirring and uneven distribution of dissolved oxygen during fermentation, which affect the fermentation effect, and to perform defoaming without affecting dissolved oxygen.
In order to achieve the purpose, the technical scheme of the invention is realized as follows:
an enzyme preparation microorganism bacterium liquid fermentation device comprises a fermentation tank and a stirring mechanism arranged in the fermentation tank; the top of the fermentation tank is provided with an air inlet pipe, the side wall of the fermentation tank is provided with a feed inlet, and the bottom of the fermentation tank is provided with a discharge outlet;
a horizontal screen is fixedly arranged at a liquid level line above the interior of the fermentation tank, a defoaming area is arranged above the horizontal screen, and a stirring area is arranged below the horizontal screen;
the stirring mechanism comprises a rotating shaft, a stirring sheet, a stirring rod, a scraping plate and a motor; the rotating shaft is vertically and rotatably arranged at the center of the fermentation tank, penetrates through the horizontal screen from bottom to top and penetrates out of the top end of the fermentation tank, and is driven by a motor arranged at the top end of the fermentation tank; a vertical screen is fixedly connected to the side wall of the rotating shaft in the upper defoaming area, and the lower end of the vertical screen is attached to the upper surface of the horizontal screen; a cooling coil is sleeved on the periphery of the rotating shaft positioned in the lower stirring area, and the water inlet end and the water outlet end of the cooling coil penetrate through the wall of the fermentation tank; fixedly connected with stirring vane and puddler in the pivot, the stirring vane is located cooling coil's inboard, and the puddler is worn out and is connected with the scraper blade from cooling coil's clearance, and the scraper blade is laminated mutually with the fermentation cylinder inboard wall.
Further, a cavity is arranged inside the rotating shaft positioned in the upper defoaming area, the top end of the rotating shaft is rotatably connected with a connecting pipe, the connecting pipe is also communicated with the lower part of the fermentation tank, and a water pump is connected onto the connecting pipe; a liquid dripping pipe communicated with the cavity is fixedly connected to the side wall of the rotating shaft, and a plurality of liquid dripping pipes are arranged on the liquid dripping pipe;
the top of pivot is still fixed the cover and is equipped with driven gear, and the top of fermentation cylinder is rotated and is equipped with the driving gear with driven gear engaged with, and the driving gear is by motor drive.
Furthermore, the lower surface of the liquid pouring pipe is fixedly connected with a plurality of defoaming needles.
Furthermore, an anchor type stirrer is also arranged on the rotating shaft below the stirring sheet; the stirring sheet is of a grid-shaped structure.
Furthermore, a return pipe is connected to the side wall of the fermentation tank, one end of the return pipe is connected to the lower portion of the fermentation tank, the other end of the return pipe is connected to the upper portion of the fermentation tank, and a high-pressure pump is arranged on the return pipe.
Furthermore, the number of the return pipes is 2, the return pipes are respectively a first return pipe and a second return pipe, the first return pipe is provided with a first high-pressure pump, and the second return pipe is provided with a second high-pressure pump; the lower end of the first reflux pipe is a liquid inlet end, and the upper end of the first reflux pipe is a liquid outlet end; the lower end of the second return pipe is a liquid outlet end, and the upper end of the second return pipe is a liquid inlet end.
The invention also provides a bacterial liquid fermentation process by using the enzyme preparation microbial liquid fermentation device, which comprises the following steps:
1) cleaning of
Closing the discharge port and the air inlet pipe, introducing pickling solution into the fermentation tank through the feed port, soaking for 30-60min, then opening the stirring mechanism, stirring and cleaning for 10-20min, and then discharging the pickling solution through the discharge port (23); then introducing purified water for washing, starting a stirring mechanism to stir while washing, and discharging the wastewater after 30-40 min;
then introducing alkaline solution into the fermentation tank and soaking for 30-60min, then starting a stirring mechanism, stirring and cleaning for 10-20min, and then discharging the alkaline solution through a discharge hole; then purified water is introduced for washing for 2-3 times, a stirring mechanism is started to stir while washing, and after washing for 30-40min each time, wastewater is discharged;
2) empty can sterilization
Closing the feed inlet and the discharge outlet, introducing pure steam into the fermentation tank through the air inlet pipe for sterilization to ensure that the temperature in the fermentation tank reaches 120-;
3) charging of
Introducing purified water for fermentation into a fermentation tank, then putting fermentation raw materials into the fermentation tank, starting a stirring mechanism to stir the materials, and then supplementing the purified water to a position 5-10cm below a liquid level line;
4) sterilization of materials
Introducing steam into the fermentation tank to make the temperature in the fermentation tank reach 100-; then introducing liquid nitrogen or cold water into the cooling coil, reducing the temperature in the tank to 30-40 ℃, and continuously stirring in the cooling process to ensure that the temperature is uniformly reduced;
5) inoculating fermentation
Adding fermentation strains into the fermentation tank, continuously stirring, introducing 30-45 deg.C sterile air into the tank to provide enough dissolved oxygen, and maintaining the temperature in the tank at 35-40 deg.C; performing primary defoaming on foam generated at the liquid level in the stirring process when the foam passes through a horizontal screen, and performing secondary defoaming on the foam passing through the horizontal screen under the action of a rotating vertical screen;
6) the fermentation is finished
And stopping culturing and harvesting the bacterial liquid after the bacterial growth enters a stable period.
Further, the step 1) comprises the steps of starting a water pump, a first high-pressure pump and a second high-pressure pump to simultaneously clean a liquid dripping pipe and a liquid dripping pipe which are communicated with the cavity in the rotating shaft, a first return pipe and a second return pipe which are connected with the side wall of the fermentation tank in the processes of pickling solution, alkaline washing solution and washing;
and 2) starting a water pump, a first high-pressure pump and a second high-pressure pump to sterilize a liquid dripping pipe and a liquid dropping pipe which are communicated with the cavity in the rotating shaft, a first return pipe and a second return pipe which are connected with the side wall of the fermentation tank simultaneously in the sterilization process.
Further, in the step 5), the first high-pressure pump and the second high-pressure pump are also started in the fermentation process, and the fermentation liquor is refluxed through the first return pipe and the second return pipe which are connected with the side wall of the fermentation tank so as to improve the blending degree of the fermentation liquor, so that the concentration of dissolved oxygen in the fermentation liquor is improved.
Further, in the step 5), a water pump is started in the fermentation process, the fermentation liquid is pumped into the cavity at the upper part of the rotating shaft through a connecting pipe communicated with the lower part of the fermentation tank, and water is dripped to the foam on the horizontal screen through a liquid dripping pipe communicated with the cavity so as to puncture the foam for defoaming.
Compared with the prior art, the enzyme preparation microbial strain liquid fermentation device and the fermentation process thereof have the following advantages:
1) aeration, agitation, metabolic processes of microorganisms, decomposition of certain components in the medium, etc. during fermentation may produce foam, and too much permanent foam is detrimental to fermentation. This tank inner liquid level line department is equipped with horizontal screen, be equipped with vertical screen cloth in the pivot of horizontal screen cloth top, the foam that the stirring produced at first can be cut by horizontal screen cloth through the horizontal screen cloth that has fine and close sieve mesh, realize the one-level defoaming, later the foam is at horizontal screen cloth upper surface gathering, vertical screen cloth rotates under the drive of pivot, further cut the defoaming to the foam, mechanical defoaming has been realized through above-mentioned structure, it is unfavorable to the fermentation to have avoided the persistence foam, can also avoid introducing the adverse effect of defoaming agent to the fermentation production simultaneously. Rabbling mechanism includes stirring arm and puddler, the stirring arm is in cooling coil's inboard, the puddler then stretches out cooling coil's clearance, it is more comprehensive to guarantee to stir, and the tip of puddler still is equipped with the scraper blade, can scrape down by the material on the fermentation cylinder inner wall, guarantee the even of stirring, owing to continuous the letting in sterilization air in fermentation process, the air complements for dissolved oxygen, the sufficiency of stirring can promote the dissolved oxygen concentration in improving the zymotic fluid, more be favorable to going on of fermentation.
(2) A cavity is arranged in the rotating shaft of the upper defoaming area, a liquid dripping pipe communicated with the cavity is fixedly connected to the side wall of the rotating shaft, a plurality of liquid dripping pipes are arranged on the liquid dripping pipe, and the cavity is also communicated with the lower part of the fermentation tank through a connecting pipe. In the fermentation process, the fermentation liquor is pumped into the cavity through the water pump, then the fermentation liquor enters the liquid dripping pipe, water is dripped to the foam through the liquid dripping pipe, the sprayed water flow or water drops can break floating bubbles, the liquid released by the bubbles recovers the settling property again, and the foam can be effectively reduced. The lower surface of the liquid pouring pipe is also fixedly connected with a plurality of defoaming needles which rotate along with the rotating shaft to generate a certain cutting effect on bubbles and play a certain defoaming role.
(3) The stirring blades are of a grid structure, so that the mixed flow degree of liquid is increased in the stirring process, and the stirring effect of the fermentation liquid is further improved.
(4) The side wall of the fermentation tank is also connected with 2 return pipes, the first return pipe and the second return pipe, the return of liquid is realized through the return pipes, and the returned liquid also has a certain stirring effect. The lower end of the first backflow pipe is a liquid inlet end, the upper end of the first backflow pipe is a liquid outlet end, the lower end of the second backflow pipe is a liquid outlet end, the upper end of the second backflow pipe is a liquid inlet end, and the two backflow pipes are different in flowing direction, so that liquid mixing is facilitated.
(5) The cooling coil is arranged for cooling, the introduction of external gas is reduced, and the introduction of external bacteria is avoided to a certain extent.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate an embodiment of the invention and, together with the description, serve to explain the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic structural diagram of an enzyme preparation microbial liquid fermentation device according to an embodiment of the invention;
FIG. 2 is a front view of an apparatus for fermenting a liquid of an enzyme preparation microorganism according to an embodiment of the present invention;
FIG. 3 is a sectional view taken along line A-A of an apparatus for fermenting a liquid of an enzyme preparation microorganism strain according to an embodiment of the present invention;
FIG. 4 is a side view of an enzyme preparation microorganism broth fermentation apparatus according to an embodiment of the present invention;
FIG. 5 is a B-B cross-sectional view of an enzyme preparation microorganism liquid fermentation apparatus according to an embodiment of the present invention;
fig. 6 is a schematic structural diagram of a stirring mechanism according to an embodiment of the present invention.
Description of reference numerals:
1-fermentation tank; 2-a rotating shaft; 3-stirring sheet; 4-a stirring rod; 5-a scraper; 6-anchor type stirrer; 7-cooling coil pipes; 8-horizontal screen mesh; 9-a liquid dripping pipe; 10-vertical screen mesh; 11-a cavity; 12-a dropping tube; 13-a driven gear; 14-a drive gear; 15-a motor; 16-a connecting tube; 17-a water pump; 18-a first return pipe; 19-a first high pressure pump; 20-a second return conduit; 21-a second high pressure pump; 22-a feed inlet; 23-a discharge hole; 24-an air inlet pipe; 25-defoaming needle.
Detailed Description
It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.
The present invention will be described in detail with reference to examples.
As shown in FIGS. 1-4, an enzyme preparation microorganism strain liquid fermentation device comprises a fermentation tank 1 and a stirring mechanism arranged inside the fermentation tank 1; the top of the fermentation tank 1 is provided with an air inlet pipe 24, the side wall of the fermentation tank 1 is provided with a feed inlet 22, and the bottom of the fermentation tank 1 is provided with a discharge outlet 23; specifically, the feed inlet 22 may be disposed below the liquid level line, so as to facilitate feeding;
a horizontal screen 8 is fixedly arranged at a liquid level line above the interior of the fermentation tank 1, a defoaming area is arranged above the horizontal screen 8, and a stirring area is arranged below the horizontal screen 8;
the stirring mechanism comprises a rotating shaft 2, a stirring sheet 3, a stirring rod 4, a scraping plate 5 and a motor 15; the rotating shaft 2 is vertically and rotatably arranged at the center of the fermentation tank 1, the rotating shaft 2 penetrates through the horizontal screen 8 from bottom to top and penetrates out of the top end of the fermentation tank 1, and the rotating shaft 2 is driven by a motor 15 arranged at the top end of the fermentation tank 1; a vertical screen 10 is fixedly connected to the side wall of the rotating shaft 2 positioned in the upper defoaming area, and the lower end of the vertical screen 10 is attached to the upper surface of the horizontal screen 8; a cooling coil 7 is sleeved on the periphery of the rotating shaft 2 positioned in the lower stirring area, and the water inlet end and the water outlet end of the cooling coil 7 penetrate through the wall of the fermentation tank; fixedly connected with stirring vane 3 and puddler 4 in the pivot 2, stirring vane 3 is located cooling coil 7's inboard, and puddler 4 is worn out and is connected with scraper blade 5 from cooling coil 7's clearance, and scraper blade 5 is laminated mutually with the inside wall of fermentation cylinder.
The radius of the area surrounded by the cooling coil 7 is 2/3 of the radius of the fermentation tank 1, 4 stirring blades 3 are uniformly distributed, and the length of the stirring blades is greater than that of the area surrounded by the cooling coil 7; the gap between the cooling coils 7 is 15 cm.
Aeration, agitation, metabolic processes of microorganisms, decomposition of certain components in the medium, etc. during fermentation may produce foam, and too much permanent foam is detrimental to fermentation. This fermentation cylinder 1 interior liquid level line department is equipped with horizontal screen 8, be equipped with vertical screen cloth 10 on the pivot 2 of horizontal screen cloth 8 top, the foam that the stirring produced at first can be through the horizontal screen cloth 8 that has fine and close sieve mesh and cut by horizontal screen cloth, realize the one-level defoaming, later the foam is at 8 upper surface aggregations of horizontal screen cloth, vertical screen cloth 10 rotates under the drive of pivot, further cut the defoaming to the foam, mechanical defoaming has been realized through above-mentioned structure, it produces unfavorable to the fermentation to have avoided the persistence foam, can also avoid introducing the adverse effect of defoaming agent to the fermentation production simultaneously. Rabbling mechanism includes stirring arm 3 and puddler 4, stirring arm 3 is in cooling coil's inboard, puddler 4 then stretches out cooling coil's clearance, it is more comprehensive to guarantee to stir, and the tip of puddler 4 still is equipped with scraper blade 5, can scrape down by the material on the fermentation cylinder inner wall, guarantee the even of stirring, owing to continuous the sterile air that lets in fermentation process, the air is complemented for dissolved oxygen, the sufficiency of stirring can promote the dissolved oxygen concentration in improving the zymotic fluid, more be favorable to going on of fermentation. The cooling coil 7 is arranged for cooling, the introduction of external gas is reduced, and the introduction of external bacteria is avoided to a certain extent.
Illustratively, as shown in fig. 3, a cavity 11 is arranged inside the rotating shaft 2 in the upper defoaming area, the top end of the rotating shaft 2 is rotatably connected with a connecting pipe 16, the connecting pipe 16 is also communicated with the lower part of the fermentation tank 1, and a water pump 17 is connected to the connecting pipe 16; a liquid dripping pipe 9 communicated with the cavity 11 is fixedly connected to the side wall of the rotating shaft 2, and a plurality of liquid dripping pipes 12 are arranged on the liquid dripping pipe 9;
the top of the rotating shaft 2 is also fixedly sleeved with a driven gear 13, the top of the fermentation tank 1 is rotatably provided with a driving gear 14 engaged with the driven gear 13, and the driving gear 14 is driven by a motor 15.
Specifically, the lower surface of the liquid pouring pipe 9 is also fixedly connected with a plurality of defoaming needles 25.
A cavity 11 is arranged in the rotating shaft 2 in the upper defoaming area, a liquid dripping pipe 9 communicated with the cavity 11 is fixedly connected to the side wall of the rotating shaft 2, a plurality of liquid dripping pipes 12 are arranged on the liquid dripping pipe 9, and the cavity 11 is also communicated with the lower part of the fermentation tank through a connecting pipe 16. In the fermentation process, the fermentation liquor is pumped into the cavity 11 through the water pump 17, then enters the liquid dripping pipe 9, drips water to the foam through the liquid dripping pipe 12, sprayed water flow or water drops can break floating bubbles, the liquid released by the bubbles recovers the settling property again, and the foam can be effectively reduced. The lower surface of the liquid pouring pipe 9 is also fixedly connected with a plurality of defoaming needles 25, and the defoaming needles rotate along with the rotating shaft to generate a certain cutting effect on bubbles and play a certain defoaming role.
Illustratively, an anchor stirrer 6 is further arranged on the rotating shaft 2 below the stirring sheet 3; the stirring sheet 3 is of a grid structure, and the grid structure enables the stirring sheet to increase the mixed flow degree of liquid in the stirring process, so that the stirring effect of the fermentation liquid is improved.
Illustratively, as shown in fig. 5, a return pipe is further connected to the sidewall of the fermentation tank 1, one end of the return pipe is connected to the lower portion of the fermentation tank 1, the other end of the return pipe is connected to the upper portion of the fermentation tank 1, and a high-pressure pump is disposed on the return pipe.
Specifically, the number of the return pipes is 2, and the return pipes are respectively a first return pipe 18 and a second return pipe 20, the first return pipe 18 is provided with a first high-pressure pump 19, and the second return pipe 20 is provided with a second high-pressure pump 21; the lower end of the first reflux pipe 18 is a liquid inlet end, and the upper end is a liquid outlet end; the lower end of the second return pipe 20 is a liquid outlet end, and the upper end is a liquid inlet end.
The side wall of the fermentation tank 1 is also connected with 2 return pipes, a first return pipe 18 and a second return pipe 20, the return of liquid is realized through the return pipes, and the returned liquid also has a certain stirring effect. The lower end of the first backflow pipe 18 is a liquid inlet end, the upper end of the first backflow pipe is a liquid outlet end, the lower end of the second backflow pipe 20 is a liquid outlet end, the upper end of the second backflow pipe is a liquid inlet end, and the two backflow pipes are different in flowing direction, so that liquid mixing is facilitated.
The bacterial liquid fermentation process of the enzyme preparation microbial liquid fermentation device comprises the following steps:
1) cleaning of
Closing the discharge port 23 and the air inlet pipe 24, introducing the pickling solution into the fermentation tank 1 through the feed port 22, soaking for 45min, then starting the stirring mechanism at the rotation speed of 1000 r/min, stirring and cleaning for 15min, and then discharging the pickling solution through the discharge port 23; then purified water is introduced for washing, a stirring mechanism is started to stir while washing, and after 30min, the wastewater is discharged;
then introducing alkaline solution into the fermentation tank 1 and soaking for 35min, then starting a stirring mechanism at the rotating speed of 1000 r/min, stirring and cleaning for 10min, and then discharging the alkaline solution through a discharge hole 23; then purified water is introduced for washing for 3 times, a stirring mechanism is started to stir while washing, and after washing for 30min each time, the wastewater is discharged;
in the processes of pickling solution, alkaline washing solution and washing, a water pump 17, a first high-pressure pump 19 and a second high-pressure pump 21 are started to simultaneously clean a liquid dripping pipe 9 and a liquid dripping pipe 12 which are communicated with a cavity 11 in a rotating shaft 2, and a first return pipe 18 and a second return pipe 20 which are connected with the side wall of a fermentation tank 1;
2) empty can sterilization
Closing the feed inlet 22 and the discharge outlet 23, introducing pure steam into the fermentation tank 1 through the air inlet pipe 24 for sterilization, and keeping the temperature in the tank at 132 ℃ for 50 min;
in the sterilization process, a water pump 17, a first high-pressure pump 19 and a second high-pressure pump 21 are started to simultaneously perform steam sterilization on a liquid pouring pipe 9 and a liquid dropping pipe 12 which are communicated with a cavity 11 in a rotating shaft 2, and a first return pipe 18 and a second return pipe 20 which are connected with the side wall of a fermentation tank 1;
3) charging of
Firstly introducing purified water for fermentation into a fermentation tank 1, then putting fermentation raw materials into the fermentation tank 1, simultaneously starting a stirring mechanism to stir the materials at the rotation speed of 1500 r/min, and then supplementing the purified water to a position 10cm below a liquid level line;
4) sterilization of materials
Introducing steam into the fermentation tank 1 to make the temperature in the tank reach 105 ℃, and keeping for 30 min; then introducing liquid nitrogen into the cooling coil 7, reducing the temperature in the tank to 30-40 ℃, and continuously stirring at the rotating speed of 1000 r/min in the cooling process to ensure that the temperature is uniformly reduced;
5) inoculating fermentation
Adding fermentation strains into the fermentation tank 1 and continuously stirring at the rotating speed of 800r/min, introducing sterilizing air of 40 +/-2 ℃ into the tank body to provide sufficient dissolved oxygen, ensuring that the temperature in the tank body is maintained at 37 ℃, and adjusting the temperature of the introduced air according to the actual temperature in the tank; the foam generated at the liquid level in the stirring process is subjected to primary defoaming when passing through the horizontal screen 8, and the foam passing through the horizontal screen 8 is subjected to secondary defoaming under the action of the rotating vertical screen 10;
in addition, in the fermentation process, a first high-pressure pump 19 and a second high-pressure pump 21 are started, and fermentation liquor is refluxed through a first return pipe 18 and a second return pipe 20 which are connected with the side wall of the fermentation tank 1 so as to improve the blending degree of the fermentation liquor, so that the concentration of dissolved oxygen in the fermentation liquor is improved;
in the fermentation process, a water pump 17 is also started, the fermentation liquor is pumped into the cavity 11 at the upper part of the rotating shaft 2 through a connecting pipe 16 communicated with the lower part of the fermentation tank 1, and water is dripped to the foam on the horizontal screen mesh 8 through a drenching pipe 9 communicated with the cavity 11 so as to puncture the foam for defoaming.
The bacteria continuously consume nutrient substances in the proliferation process, the oxygen demand is increased along with the increase of the number of bacteria, so that the nutrients such as carbon sources and nitrogen sources are required to be supplemented and the dissolved oxygen is required to be increased according to needs, and meanwhile, the pH value is changed along with the increase of metabolites, so that the pH value is corrected according to needs.
6) The fermentation is finished
Sampling in the culture process for pure detection.
And when the bacteria grow to enter a stable period, stopping culturing and harvesting the bacteria liquid.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (2)

1. The utility model provides an enzyme preparation microbial inoculum liquid fermenting installation which characterized in that: comprises a fermentation tank (1) and a stirring mechanism arranged in the fermentation tank (1); the top of the fermentation tank (1) is provided with an air inlet pipe (24), the side wall of the fermentation tank (1) is provided with a feed inlet (22), and the bottom of the fermentation tank (1) is provided with a discharge outlet (23);
a horizontal screen (8) is fixedly arranged at a liquid level line above the interior of the fermentation tank (1), a defoaming area is arranged above the horizontal screen (8), and a stirring area is arranged below the horizontal screen (8);
the stirring mechanism comprises a rotating shaft (2), a stirring sheet (3), a stirring rod (4), a scraping plate (5) and a motor (15); the rotating shaft (2) is vertically and rotatably arranged at the center of the fermentation tank (1), the rotating shaft (2) penetrates through the horizontal screen (8) from bottom to top and penetrates out of the top end of the fermentation tank (1), and the rotating shaft (2) is driven by a motor (15) arranged at the top end of the fermentation tank (1); a vertical screen (10) is fixedly connected to the side wall of the rotating shaft (2) positioned in the upper defoaming area, and the lower end of the vertical screen (10) is attached to the upper surface of the horizontal screen (8); a cooling coil (7) is sleeved on the periphery of the rotating shaft (2) positioned in the lower stirring area, and the water inlet end and the water outlet end of the cooling coil (7) penetrate through the wall of the fermentation tank; the stirring blade (3) and the stirring rod (4) are fixedly connected to the rotating shaft (2), the stirring blade (3) is located on the inner side of the cooling coil (7), the stirring rod (4) penetrates out of the gap of the cooling coil (7) and is connected with the scraping plate (5), and the scraping plate (5) is attached to the inner side wall of the fermentation tank;
a cavity (11) is arranged in the rotating shaft (2) positioned in the upper defoaming area, the top end of the rotating shaft (2) is rotatably connected with a connecting pipe (16), the connecting pipe (16) is also communicated with the lower part of the fermentation tank (1), and a water pump (17) is connected on the connecting pipe (16); a liquid dripping pipe (9) communicated with the cavity (11) is fixedly connected to the side wall of the rotating shaft (2), and a plurality of liquid dripping pipes (12) are arranged on the liquid dripping pipe (9);
the top end of the rotating shaft (2) is also fixedly sleeved with a driven gear (13), the top end of the fermentation tank (1) is rotatably provided with a driving gear (14) meshed with the driven gear (13), and the driving gear (14) is driven by a motor (15);
the lower surface of the liquid pouring pipe (9) is also fixedly connected with a plurality of defoaming needles (25);
an anchor stirrer (6) is also arranged on the rotating shaft (2) below the stirring sheet (3); the stirring sheet (3) is of a grid-shaped structure;
a return pipe is also connected to the side wall of the fermentation tank (1), one end of the return pipe is connected to the lower part of the fermentation tank (1), the other end of the return pipe is connected to the upper part of the fermentation tank (1), and a high-pressure pump is arranged on the return pipe;
the number of the return pipes is 2, the return pipes are respectively a first return pipe (18) and a second return pipe (20), the first return pipe (18) is provided with a first high-pressure pump (19), and the second return pipe (20) is provided with a second high-pressure pump (21); the lower end of the first reflux pipe (18) is a liquid inlet end, and the upper end of the first reflux pipe is a liquid outlet end; the lower end of the second return pipe (20) is a liquid outlet end, and the upper end of the second return pipe is a liquid inlet end.
2. A bacterial liquid fermentation process using the enzyme preparation microbial liquid fermentation device of claim 1, characterized in that: the process comprises the following steps:
1) cleaning of
Closing the discharge hole (23) and the air inlet pipe (24), introducing pickling solution into the fermentation tank (1) through the feed hole (22), soaking for 30-60min, then opening the stirring mechanism, stirring and cleaning for 10-20min, and then discharging the pickling solution through the discharge hole (23); then introducing purified water for washing, starting a stirring mechanism to stir while washing, and discharging the wastewater after 30-40 min;
then introducing alkaline solution into the fermentation tank (1) and soaking for 30-60min, then starting a stirring mechanism, stirring and cleaning for 10-20min, and then discharging the alkaline solution through a discharge hole (23); then purified water is introduced for washing for 2-3 times, a stirring mechanism is started to stir while washing, and after washing for 30-40min each time, wastewater is discharged;
the step 1) also comprises the steps of starting a water pump (17), a first high-pressure pump (19) and a second high-pressure pump (21) to simultaneously clean a liquid dripping pipe (9) and a liquid dripping pipe (12) which are communicated with the cavity (11) in the rotating shaft (2), a first return pipe (18) and a second return pipe (20) which are connected with the side wall of the fermentation tank (1) in the processes of pickling solution, alkaline washing solution and washing;
2) empty can sterilization
Closing the feed inlet (22) and the discharge outlet (23), introducing pure steam into the fermentation tank (1) through the air inlet pipe (24) for sterilization, and keeping the temperature in the tank at 120-;
the step 2) also comprises the step of simultaneously sterilizing a liquid pouring pipe (9) and a liquid dropping pipe (12) which are communicated with the cavity (11) in the rotating shaft (2), a first return pipe (18) and a second return pipe (20) which are connected with the side wall of the fermentation tank (1) by starting a water pump (17), a first high-pressure pump (19) and a second high-pressure pump (21) in the sterilization process;
3) charging of
Purified water for fermentation is firstly introduced into a fermentation tank (1), then fermentation raw materials are put into the fermentation tank (1), a stirring mechanism is started to stir the materials, and then the purified water is supplemented to a position 5-10cm below a liquid level line;
4) sterilization of materials
Introducing steam into the fermentation tank (1) to make the temperature in the fermentation tank reach 100-; then introducing liquid nitrogen or cold water into the cooling coil (7), reducing the temperature in the tank to 30-40 ℃, and continuously stirring in the cooling process to ensure that the temperature is uniformly reduced;
5) inoculating fermentation
Adding fermentation strains into the fermentation tank (1) and continuously stirring, and simultaneously introducing 30-45 ℃ sterile air into the tank body to provide sufficient dissolved oxygen and ensure that the temperature in the tank body is maintained at 35-40 ℃; the foam generated at the liquid level in the stirring process is subjected to primary defoaming when passing through the horizontal screen (8), and the foam passing through the horizontal screen (8) is subjected to secondary defoaming under the action of the rotating vertical screen (10);
in the fermentation process, a first high-pressure pump (19) and a second high-pressure pump (21) are started, and fermentation liquor is refluxed through a first return pipe (18) and a second return pipe (20) which are connected with the side wall of the fermentation tank (1) so as to improve the blending degree of the fermentation liquor, so that the concentration of dissolved oxygen in the fermentation liquor is improved;
in the fermentation process, a water pump (17) is started, fermentation liquor is pumped into a cavity (11) at the upper part of the rotating shaft (2) through a connecting pipe (16) communicated with the lower part of the fermentation tank (1), and water is dripped to foam on the horizontal screen (8) through a drenching pipe (9) communicated with the cavity (11) to puncture the foam for defoaming;
6) the fermentation is finished
And stopping culturing and harvesting the bacterial liquid after the bacterial growth enters a stable period.
CN202011521870.5A 2020-12-22 2020-12-22 Enzyme preparation microbial strain liquid fermentation device and fermentation process thereof Active CN112251338B (en)

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