CN1134215C - Germicide and its preparation - Google Patents
Germicide and its preparation Download PDFInfo
- Publication number
- CN1134215C CN1134215C CNB981082408A CN98108240A CN1134215C CN 1134215 C CN1134215 C CN 1134215C CN B981082408 A CNB981082408 A CN B981082408A CN 98108240 A CN98108240 A CN 98108240A CN 1134215 C CN1134215 C CN 1134215C
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- japanese apricot
- extract
- plum
- remover
- water
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Abstract
To obtain an antimicrobial agent which can contribute to the effective utilization of ume trees and is excellent in safety. Shoots which develop rapidly at the expense of the rest of ume trees are pruned, and the shoots are processed flake-like, steamed at 130 deg.C with steam and then immersed into pure water under cooling to extract the shoot extract in the pure water After extraction, the shoots are filtered off and the filtrate is collected as the extract. Since the extract possesses excellent antimicrobial activity and no problem in the safety, it can be used as an antimicrobial. Further, this extract possesses preferable ume fragrance. Furthermore, this antimicrobial is water-soluble and therefore can be used as a food antiseptic and a water coating. In addition, it is usable as a detergent for removing the slime in the kitchen and the like, an antimicrobial material, etc., and can be widely applied.
Description
The invention relates to antibacterial agent and manufacture method thereof, more particularly, the invention relates to the antibacterial agent and the manufacture method thereof that contain the extract that from the branch of Japanese apricot etc., extracts.
For a long time, for the plum that obtains to eat, people have planted a large amount of Japanese apricots.In the cultivation process of Japanese apricot, all will prune water sprout annual autumn, and pruning rate is very big, several kilotons arranged to several ten thousand tons more than every year.At present, these are pruned the water sprout that gets off and are not effectively utilized, and have all fallen as castoff burning.In addition, the shell after medicinal extract that obtains green plum and plum meat has all been thrown away basically.It is very disadvantageous doing resources effective utilization and environmental protection like this.Effective utilization for Japanese apricot is not limited only to above-mentioned water sprout, for example also relates to trunk, branch, leaf, root of Japanese apricot or the like.
In addition, be accompanied by the improvement of living environment and people change in recent years, develop various antibacterial agents and have the goods of antibiotic property health concept.These antibacterial agents and antibiotic property goods must be considered the safety to human body.
The objective of the invention is, effectively utilize Mei Mu, antibacterial agent and manufacture method thereof with good safety are provided.
To achieve these goals, antibacterial agent of the present invention contains the extract that extracts from the trunk, branch, leaf, root, pulp, shell and the kernel that are selected from Japanese apricot at least a.
The inventor has carried out a series of research repeatedly in order to effectively utilize Japanese apricot, found that, the material that extracts in the moisture by water sprout of Japanese apricot etc. has antibacterial action, thereby has finished the present invention.In addition, the inventor has also carried out same research to other trees such as Citrus, and the result compares with the extract of other trees, and the antibacterial action of the extract of the water sprout of Japanese apricot etc. is extremely strong, can fully satisfy practical needs.In addition, there is not safety issue in the said extracted thing of Japanese apricot to human body, and has the fragrance of plum, and therefore, not only safety is good to use the antibacterial agent of the present invention that this extract obtains, and has pleasant fragranced.In addition, antibacterial agent of the present invention also has the character that easily is dissolved in water, and it is had many uses, for example as hereinafter described in, can be used as food preservative, perhaps be used for adding to water paint.
Antibacterial agent of the present invention can be made of said extracted thing (100%) fully.In addition, " antibiotic " described in the present invention comprises the implication of " antibacterial " and " sterilization " two aspects.
In the present invention, the said extracted thing preferably contains catechin, because catechin is the material with antibacterial action.In said extracted thing of the present invention, the material that demonstrates antibacterial action is not limited to catechin.In the present invention, the form of antibacterial agent is preferably Powdered or aqueous.
The manufacture method of antibacterial agent of the present invention has 4 kinds of manufacture methods described below.
At first, the 1st kind of manufacture method of the present invention is with being selected from least a flakelike powder that is processed into of trunk, branch, leaf, root, pulp, shell and the kernel of Japanese apricot, to steam with heat steam, put into water dipping, cooling then, this aqueous solution is reclaimed as extract.
The 2nd kind of manufacture method of the present invention be, will be selected from least a flakelike powder that is processed into of trunk, branch, leaf, root, pulp, shell and the kernel of Japanese apricot, and water boils and boils, and cooling is reclaimed the cooled aqueous solution as extract then.
The 3rd kind of manufacture method of the present invention be, with being selected from least a flakelike powder that is processed into of trunk, branch, leaf, root, pulp, shell and the kernel of Japanese apricot, puts into organic solvent and flood, and the organic solvent behind the dipping is reclaimed as extract.
The 4th kind of manufacture method of the present invention be, with being selected from least a flakelike powder that is processed into of trunk, branch, leaf, root, pulp, shell and the kernel of Japanese apricot, it carried out destructive distillation, and distillate is reclaimed as extract.In addition, above-mentioned destructive distillation comprises carbonization.The carbonization of timber etc. is to heat under the situation of secluding air.In this carbonisation, its distillate is reclaimed as extract.
Antibiotic property goods of the present invention contain the antibacterial agent of the invention described above.Said products is at least a in fiber, cloth, plate, paper, resin and the wall preferably.
Remover of the present invention contains the antibacterial agent of the invention described above.Antibacterial agent of the present invention except antibacterial action, can also be removed the mucus that produces in lavatory, kitchen, the bathroom etc.
Antibiotic property coating of the present invention contains the antibacterial agent of the invention described above.Because the dissolubility of extract of the present invention in water is very high, therefore antibacterial agent of the present invention is allocated in the water paint and can be given its antibiotic property.
Food of the present invention contains the antibacterial agent of the invention described above with preservative.As mentioned above, antibacterial agent of the present invention contains the extract of the water sprout of Japanese apricot etc., does not have the safety issue to human body, therefore also can add in the food.
The working of an invention scheme
Antibacterial agent of the present invention for example can adopt above-mentioned the 1st kind of method, by the following stated manufacturing.
At first, water sprout of Japanese apricot etc. is processed into flakelike powder.The size of this flakelike powder is the sawdust size normally.Use the water sprout of pruning.Being processed into flakelike powder for example can use timber to carry out with cutting element.
Subsequently, this flakelike powder is steamed with heat steam, typical temperature is 100-200 ℃, preferably about 130 ℃.Generally be 1 minute-3 hours heat time heating time.
The heating back is the dipping cooling in water.In cooling procedure, from water sprout etc., extract antimicrobial component.Normally about 0-30 ℃ of chilling temperature, preferably 0-20 ℃.The water of cooling usefulness has no particular limits, the water that can drink such as running water preferably, preferably pure water such as distilled water.
Then, filter above-mentioned flakelike powder, filtrate is reclaimed as extract with filter.This extract can directly be used as antibacterial agent, also can add behind other composition as antibacterial agent.Calculate with dry weight, the active ingredient concentration in the said extracted liquid is 0.01-99.99% (weight) normally, preferably 1-30% (weight).When the active ingredient concentration of extract is lower than normal concentration, extract can be boiled dry, concentrate, when surpassing normal concentration, can be diluted with pure water.
This extract can directly be used as aqueous antibacterial agent, and it is powdered also can to pass through processings such as atomized drying, freeze drying.Above-mentioned processing can be used normally used spray dryer or freeze drier.
The following describes and adopt above-mentioned the 2nd kind of method to make the example of antibacterial agent.
At first, as stated above water sprout of Japanese apricot etc. is processed into flakelike powder.Boil with hot water and to boil.The condition of boiling of boiling is normally boiled under 80-100 ℃ and is boiled 1 minute-3 hours, preferably boils under 95-100 ℃ and boils 30 minutes-1 hour.With above-mentioned same, the hot water that boils usefulness that boils preferably adopts pure water.Boil when boiling, from water sprout etc., extract antimicrobial component.Boil and boil the back cooling.The method of cooling, except cooling off naturally, the method that for example can also adopt that making boils and boil the outer wall of the still of usefulness to contact with cold water.Use if boil when boiling to have adjustment and boil still with boiling of chuck, can make boils boils with refrigerating work procedure and carries out smoothly.Water sprout after extracting with separation such as filters then etc. reclaim extract.After this operation is identical with above-mentioned the 1st kind of manufacture method.
The following describes and adopt above-mentioned the 3rd kind of method to make the example of antibacterial agent.
Water sprout with Japanese apricot is processed into flakelike powder at first as stated above, then it is flooded in organic solvent.The kind of organic solvent has no particular limits, and for example can enumerate methyl alcohol, ethanol, hexane, acetone, ethyl acetate, glycerine, propane diols etc., wherein, considers preferentially to select for use ethanol from the angle of safety and acquisition easily.In addition, the organic solvent that is used to extract is not limited to a kind, also can be more than 2 kinds and usefulness, perhaps mix use with water.For example, in the occasion of alcohol with the mixed liquor of water, the concentration of above-mentioned alcohol generally is 20-99.95% (weight), preferably 70-99.95% (weight).Dip time generally is 1 minute-3 hours.The temperature of dipping has no particular limits, and for example can be room temperature.During dipping, from water sprout etc., extract antimicrobial component.Water sprout after will extracting with filter etc. then separates, and reclaims extract.After this operation is identical with above-mentioned the 1st kind of manufacture method.
The following describes and adopt above-mentioned the 4th kind of method to make the example of antibacterial agent.
At first, the water sprout with Japanese apricot is processed into flakelike powder as stated above, then, under the situation of secluding air it is carried out heat treated (destructive distillation).Normally 90-300 ℃ of heating-up temperature, generally be 1-3 hour heat time heating time.Destructive distillation for example can use retort etc. to carry out.The liquid that will distillate through destructive distillation is as extract, and reclaimed after above-mentioned water sprout etc. separates.The extract that destructive distillation obtains, the concentration of active ingredient is higher.After this operation is identical with above-mentioned the 1st kind of manufacture method.
The following describes the purposes of this antibacterial agent.
At first, aqueous antibacterial agent for example can be used as the spray-like antibacterial agent, with the spray-like antibacterial agent to the spraying of food such as the wall of dwelling house, furniture, commodity, meat, fish, vegetables, can carry out degerming to it, both can endowing antibacterial, can give the pleasant fragrance of plum again, like this, except antisepsis, also can make it to have the fragrance of plum.In addition, it is added in the water paint can also obtain the antibiotic property water paint.This antibiotic property water paint, long preservation can be not corrupt yet, are coated with the article of this coating in addition, not only have antibiotic property and also have aromatic odor.
Secondly, Powdered antibacterial agent of the present invention can be used as remover, for example is used to remove the mucus that kitchen, bathroom, lavatory etc. are located to produce.Use this remover, all right degerming makes maintenance health such as kitchen when removing mucus, and is same with general remover, also can allocate surfactant or detergency builder in this remover.In addition, this Powdered antibacterial agent can use when making the antibiotic property goods, for example in the occasion of making the antibiotic property synthetic fibers, can add above-mentioned Powdered antibacterial agent in material resin.
The following describes embodiment.
Embodiment 1
Use the timber parting tool that the water sprout of Japanese apricot is cut off, obtain the flakelike powder of water sprout.With steam above-mentioned flakelike powder was steamed 3 hours down at 130 ℃, immerse cooling in about 20 ℃ cold water (pure water) then.Remove above-mentioned flakelike powder with filter, filtrate is reclaimed as extract.
Adopt colony counting method described below that resulting extract is carried out the antibiotic property test, result of the test is as follows.
[test strain]
Escherichia coli: bacillus coli (E.coli) IFO 3301
Staphylococcus aureus: staphylococcus aureus (S.aureus) IFO12732
MRSA: staphylococcus aureus (S.aureus) IID1677
Pseudomonas aeruginosa: pseudomonas aeruginosa (P.aeruginosa) IFO13275
[the bacterium number is measured and is used medium]
SCDLP agar medium (Nihon Pharmaceutical Co., Ltd.'s manufacturing)
[preparation of bacterium liquid]
(1) Escherichia coli, staphylococcus aureus and MRSA
Make in plain agar slant medium (Rong Yan chemistry society make), 35 ℃ of thalline of cultivating 16-20 hour test strain down swim in sterilization 1/100 concentration ordinary broth, make the bacterium number of every 1ml be about 10
6
(2) pseudomonas aeruginosa
Make in plain agar slant medium (Rong Yan chemistry society make), 35 ℃ of thalline of cultivating 16-20 hour test strain down swim in sterilization 1/1000 concentration ordinary broth, make the bacterium number of every 1ml be about 10
6
[test operation]
Said extracted liquid is coated on makes it dry on the surface of plastic plate (long 50mm * wide 50mm), be used as sample.Inoculation 0.5ml bacterium liquid behind the sticking polyethylene film, was preserved 6 hours down at 35 ℃ on this sample, gave birth to residual bacterium with above-mentioned SCDLP medium 10ml wash-out.Measure with medium, adopt agar plate method (35 ℃, 2 days) to measure the bacterium number of every 1ml eluent with the bacterium number, be converted into the aerobic plate count of each sheet sample.In addition, test in contrast, the bacterium number of the same quantity of inoculating in inoculation and the said sample in culture dish is operated equally with above-mentioned.Bacterium number when preserving beginning with tester as benchmark.Such operation is carried out 2 times each sample or tester.
[Escherichia coli]
Tester 1 clump count:
When just beginning to preserve 5.3 * 10
5, preserve after 6 hours 6.2 * 10
4
When just beginning to preserve 7.1 * 10
5, preserve after 6 hours 7.0 * 10
4
Sample 1 clump count (preserving after 6 hours): 9.0 * 10
3
6.7×10
4
Sample 2 clump counts (preserving after 6 hours):＜10
＜10
[staphylococcus aureus]
Tester 1 clump count:
When just beginning to preserve 5.4 * 10
5, preserve after 6 hours 1.4 * 10
5
When just beginning to preserve 5.3 * 10
5, preserve after 6 hours 3.1 * 10
5
Sample 1 clump count (preserving after 6 hours): 1.1 * 10
5
9.0×10
4
Sample 2 clump counts (preserving after 6 hours): 5.6 * 10
4
4.5×10
4
[MRSA]
Tester 1 clump count:
When just beginning to preserve 3.3 * 10
5, preserve after 6 hours 1.2 * 10
4
When just beginning to preserve 5.7 * 10
5, preserve after 6 hours 4.7 * 10
4
Sample 1 clump count (preserving after 6 hours): 1.2 * 10
5
9.6×10
4
Sample 2 clump counts (preserving after 6 hours): 8.9 * 10
3
8.8×10
3
[pseudomonas aeruginosa]
Tester 1 clump count:
When just beginning to preserve 3.2 * 10
5, preserve after 6 hours 1.2 * 10
4
When just beginning to preserve 3.1 * 10
5, preserve after 6 hours 7.6 * 10
5
Sample 1 clump count (preserving after 6 hours): 1.2 * 10
5
1.5×10
5
Sample 2 clump counts (preserving after 6 hours): 3.4 * 10
3
9.7×10
2
By The above results as can be seen, the extract of the water sprout of Japanese apricot has good antibacterial action.
As mentioned above, antibacterial agent of the present invention contains the extract that extracts from the trunk, branch, leaf, root, pulp, shell and the kernel that are selected from Japanese apricot at least a.This extract has good antibacterial action, and does not have safety issue.In addition, antibacterial agent of the present invention also has the pleasant fragranced of plum.Therefore use antibacterial agent of the present invention can increase the health of daily life and comfortable.Therefore antibacterial agent water soluble of the present invention is applicable to food preservative and antibiotic property water paint etc.Use antibacterial agent of the present invention can make goods such as cloth or plate have antibiotic property.In addition, antibacterial agent of the present invention is to be extracted by the discarded object that Japanese apricots such as water sprout are cultivated, and therefore can realize the resources effective utilization and help environmental protection.
Claims (8)
1. one kind contains and is selected from least a purposes that is used for kitchen, lavatory and bathing pool by the remover of the extract of at least a extraction in trunk, Japanese apricot branch, Japanese apricot leaf, Japanese apricot root, plum pulp, plum shell and the plum kernel of Japanese apricot.
2. the described purposes of claim 1, wherein, described extract contains catechin.
3. claim 1 or 2 described purposes, wherein, the shape of described remover is Powdered.
4. claim 1 or 2 described purposes, wherein, the shape of described remover is aqueous.
5. claim 1 or 2 described purposes, wherein said remover is prepared by following manufacture method,
With at least a flakelike powder that is processed into that is selected from trunk, Japanese apricot branch, Japanese apricot leaf, Japanese apricot root, plum pulp, plum shell and the plum kernel of Japanese apricot;
With this flakelike powder of heating steam boiling;
Impregnated in the water then, cool off;
This aqueous extract is reclaimed as extract.
6. claim 1 or 2 described purposes, wherein said remover by following manufacture method preparation,
With at least a flakelike powder that is processed into that is selected from trunk, Japanese apricot branch, Japanese apricot leaf, Japanese apricot root, plum pulp, plum shell and the plum kernel of Japanese apricot;
Described flakelike powder water boiled boil back cooling;
Cooled aqueous extract is reclaimed as extract.
7. claim 1 or 2 described purposes, wherein said remover is prepared by following manufacture method,
With at least a flakelike powder that is processed into that is selected from trunk, Japanese apricot branch, Japanese apricot leaf, Japanese apricot root, plum pulp, plum shell and the plum kernel of Japanese apricot;
Described flakelike powder be impregnated in the organic solvent;
Organic solvent behind the dipping is reclaimed as extract.
8. claim 1 or 2 described purposes, wherein said remover is prepared by following manufacture method,
With at least a flakelike powder that is processed into that is selected from trunk, Japanese apricot branch, Japanese apricot leaf, Japanese apricot root, plum pulp, plum shell and the plum kernel of Japanese apricot;
Described flakelike powder is carried out destructive distillation,
This distillate is reclaimed as extract.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP98844/97 | 1997-04-16 | ||
| JP98844/1997 | 1997-04-16 | ||
| JP9884497 | 1997-04-16 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1197603A CN1197603A (en) | 1998-11-04 |
| CN1134215C true CN1134215C (en) | 2004-01-14 |
Family
ID=14230568
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB981082408A Expired - Fee Related CN1134215C (en) | 1997-04-16 | 1998-04-16 | Germicide and its preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1134215C (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101190280B (en) * | 2006-11-27 | 2012-05-23 | 杭州尤美特科技有限公司 | Use of extract of non-fruit part of prune tree |
| CN101190281B (en) * | 2006-11-27 | 2012-01-11 | 杭州尤美特科技有限公司 | Prune tree extract and preparation method and application thereof |
-
1998
- 1998-04-16 CN CNB981082408A patent/CN1134215C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1197603A (en) | 1998-11-04 |
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