CN113416101A - Cytidine triphosphate biological organic liquid fertilizer formula and production method thereof - Google Patents

Cytidine triphosphate biological organic liquid fertilizer formula and production method thereof Download PDF

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Publication number
CN113416101A
CN113416101A CN202110708058.1A CN202110708058A CN113416101A CN 113416101 A CN113416101 A CN 113416101A CN 202110708058 A CN202110708058 A CN 202110708058A CN 113416101 A CN113416101 A CN 113416101A
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Prior art keywords
cytidine triphosphate
sulfate
liquid fertilizer
organic liquid
biological organic
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CN202110708058.1A
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Inventor
杨志贤
杨进
陈红
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Guizhou Hongyang Bio Organic Fertilizer Co ltd
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Guizhou Hongyang Bio Organic Fertilizer Co ltd
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Priority to CN202110708058.1A priority Critical patent/CN113416101A/en
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Fertilizers (AREA)

Abstract

The invention discloses a cytidine triphosphate biological organic liquid fertilizer formula and a production method thereof, wherein the cytidine triphosphate biological organic liquid fertilizer comprises the following material components: 300kg of fish with squama, 10kg of high-activity rhizobium strain rich in azotobacter, 5kg of EM bacterial liquid, 5kg of trace elements, 5kg of cytidine triphosphate aqueous solution and 20kg of urea; the method comprises the following steps: step 1: pulping the prepared 300kg of fish with scaly nails; step 2: filling 300kg of prepared fish paste into ozone for disinfection and sterilization; and step 3: 1kg of cytidine triphosphate aqueous solution, 10kg of high-activity rhizobium strain rich in azotobacter, 5kg of EM bacterial liquid, trace elements and 20kg of urea; and step 3: 4kg of cytidine triphosphate aqueous solution was added to one ton of the organic biological stock solution prepared by fermentation. The biological organic liquid fertilizer prepared by mixed fermentation can fully and effectively utilize organic liquid, thereby improving the using effect of the biological organic liquid fertilizer.

Description

Cytidine triphosphate biological organic liquid fertilizer formula and production method thereof
Technical Field
The invention relates to the technical field of organic liquid fertilizer preparation, in particular to a cytidine triphosphate biological organic liquid fertilizer formula and a production method thereof.
Background
Cytidine triphosphate is synthesized by CTP ammonialyase catalysis, and is nucleotide which combines trimolecular phosphate on ribose-5' -OH group of cytidine. There are two high energy phosphate bonds. Is one of the direct precursors for RNA biosynthesis. Participate in the synthesis of certain polysaccharides. Synthesized enzymatically by the amination of CTP. In biosynthesis of phospholipids such as lecithin and phosphatidylethanolamine, CTP acts on phosphorylcholine and phosphatidylethanolamine to enzymatically produce cytidine diphosphate choline and cytidine diphosphate ethanolamine.
The organic liquid fertilizer is a novel fertilizer with high concentration, high fertilizer efficiency, multifunction and full nutrition synergy. Compared with the traditional fertilizer, the fertilizer can be absorbed by plants in a very short time, avoids loss, is simple and convenient to apply, and is easy to transport and store.
However, the existing biological organic liquid fertilizer has a simple composition formula, and cannot fully utilize organic liquid, so that the application effect of the biological organic liquid fertilizer is influenced.
Disclosure of Invention
1. Technical problem to be solved
The invention aims to solve the problems that the composition formula of a biological organic liquid fertilizer in the prior art is simple and organic liquid cannot be fully utilized, and provides a cytidine triphosphate biological organic liquid fertilizer formula and a production method thereof.
2. Technical scheme
In order to achieve the purpose, the invention adopts the following technical scheme:
the cytidine triphosphate biological organic liquid fertilizer formula comprises the following material components: 310kg of fish 290 with squama, 9-11kg of high-activity rhizobium strain rich in azotobacter, 4-6kg of EM bacterial liquid, 5-6kg of trace elements, cytidine triphosphate aqueous solution and 19-21kg of urea.
Preferably, the material comprises the following components: 300kg of fish with squama, 10kg of high-activity rhizobium strain rich in azotobacter, 5kg of EM bacterial liquid, 5.5kg of trace elements, 5.5kg of cytidine triphosphate aqueous solution and 20kg of urea.
Preferably, the material comprises the following components: 290kg of fish with squama, 11kg of high-activity rhizobium strain rich in azotobacter, 4kg of EM bacterial liquid, 5kg of trace elements, cytidine triphosphate aqueous solution and 21kg of urea.
Preferably, the material comprises the following components: 310kg of fish with squama, 9kg of high-activity rhizobia strain rich in azotobacter, 6kg of EM bacterial liquid, 6kg of trace elements, 6kg of cytidine triphosphate aqueous solution and 19kg of urea.
Preferably, the trace elements comprise the following material components: 3kg of boric acid, 1.5kg of ammonium molybdate, 2.5kg of ferrous sulfate, 1.6kg of magnesium sulfate, 3kg of ammonium sulfate, 0.25kg of manganese sulfate, 0.01kg of zinc sulfate and 4.5kg of potassium dihydrogen sulfate.
The invention also provides a production method of the cytidine triphosphate biological organic liquid fertilizer formula, which comprises the following steps of:
step 1: step 1: pulping prepared 300kg of fish with squama, and stirring uniformly;
step 2: filling 300kg of prepared fish paste into ozone for disinfection and sterilization, and completing disinfection and sterilization when the fish paste is whitened;
and step 3: 1kg of cytidine triphosphate aqueous solution, 10kg of high-activity rhizobium strain rich in azotobacter, 5kg of EM bacterial liquid, trace elements and 20kg of urea are sealed and fermented;
and 4, step 4: adding 4kg of cytidine triphosphate aqueous solution into one ton of organic biological stock solution prepared by fermentation, adding 3kg of boric acid, 1.5kg of ammonium molybdate, 2.5kg of ferrous sulfate, 1.6kg of magnesium sulfate, 3kg of ammonium sulfate, 0.25kg of manganese sulfate, 0.01kg of zinc sulfate and 4.5kg of potassium dihydrogen sulfate, and fully and uniformly stirring to obtain the cytidine triphosphate biological organic liquid fertilizer.
Preferably, the fish paste is left standing in the natural state in the step 1 until the ozone volatilization is completed.
Preferably, the pure organic biological raw liquid fertilizer in the step 2 is hermetically fermented for 240 hours under the conditions of pH5.5 and 30 ℃.
3. Advantageous effects
Compared with the prior art, the invention has the advantages that:
according to the invention, a certain amount of fish with squama, high-activity rhizobia strains rich in azotobacter, EM bacterial liquid, trace elements, cytidine triphosphate aqueous solution and urea are mixed and fermented to prepare the biological organic liquid fertilizer, so that the organic liquid can be fully and effectively utilized, and the using effect of the biological organic liquid fertilizer is improved.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments.
Example 1:
the cytidine triphosphate biological organic liquid fertilizer formula comprises the following material components: 310kg of fish 290 with squama, 9-11kg of high-activity rhizobium strain rich in azotobacter, 4-6kg of EM bacterial liquid, 5-6kg of trace elements, cytidine triphosphate aqueous solution and 19-21kg of urea.
In the invention, the trace elements comprise the following material components: 3kg of boric acid, 1.5kg of ammonium molybdate, 2.5kg of ferrous sulfate, 1.6kg of magnesium sulfate, 3kg of ammonium sulfate, 0.25kg of manganese sulfate, 0.01kg of zinc sulfate and 4.5kg of potassium dihydrogen sulfate.
The invention discloses a production method of a cytidine triphosphate biological organic liquid fertilizer formula, which is calculated by preparing one ton of cytidine triphosphate biological organic liquid fertilizer and comprises the following steps:
step 1: pulping prepared 300kg of fish with squama, adding 660kg of water, stirring uniformly, then adding ozone, after the fish paste is whitened and has no fishy smell, completing disinfection and sterilization, and standing the fish paste in a natural state until the ozone is volatilized;
step 2: adding 10kg of high-activity rhizobia strain rich in azotobacter, 5kg of EM bacterial liquid and 1.5kg of cytidine triphosphate aqueous solution, adding 0.4kg of ammonium molybdate, 0.25kg of magnesium sulfate, 0.3kg of ferrous sulfate, 0.4kg of ammonium sulfate, 0.15kg of monopotassium phosphate, 0.06kg of sodium chloride, 2.5ppm of manganese sulfate, 0.025ppm of zinc sulfate and 20kg of urea, uniformly stirring, and carrying out sealed fermentation at the conditions of pH5.5 and 30 ℃ for 240 hours to obtain a pure organic biological stock solution fertilizer rich in azotobacter and EM bacteria;
and step 3: adding 4kg of cytidine triphosphate aqueous solution into one ton of organic biological stock solution prepared by fermentation, adding 3kg of boric acid, 1.5kg of ammonium molybdate, 2.5kg of ferrous sulfate, 1.6kg of magnesium sulfate, 3kg of ammonium sulfate, 0.25kg of manganese sulfate, 0.01kg of zinc sulfate and 4.5kg of potassium dihydrogen sulfate, and fully and uniformly stirring to obtain the cytidine triphosphate biological organic liquid fertilizer.
Example 2:
the cytidine triphosphate biological organic liquid fertilizer formula comprises the following material components: 300kg of fish with squama, 10kg of high-activity rhizobium strain rich in azotobacter, 5kg of EM bacterial liquid, 5.5kg of trace elements, 5.5kg of cytidine triphosphate aqueous solution and 20kg of urea.
In the invention, the trace elements comprise the following material components: 3kg of boric acid, 1.5kg of ammonium molybdate, 2.5kg of ferrous sulfate, 1.6kg of magnesium sulfate, 3kg of ammonium sulfate, 0.25kg of manganese sulfate, 0.01kg of zinc sulfate and 4.5kg of potassium dihydrogen sulfate.
The invention discloses a production method of a cytidine triphosphate biological organic liquid fertilizer formula, which is calculated by preparing one ton of cytidine triphosphate biological organic liquid fertilizer and comprises the following steps:
step 1: pulping prepared 300kg of fish with squama, adding 660kg of water, stirring uniformly, then adding ozone, after the fish paste is whitened and has no fishy smell, completing disinfection and sterilization, and standing the fish paste in a natural state until the ozone is volatilized;
step 2: adding 10kg of high-activity rhizobia strain rich in azotobacter, 5kg of EM bacterial liquid and 1.5kg of cytidine triphosphate aqueous solution, adding 0.4kg of ammonium molybdate, 0.25kg of magnesium sulfate, 0.3kg of ferrous sulfate, 0.4kg of ammonium sulfate, 0.15kg of monopotassium phosphate, 0.06kg of sodium chloride, 2.5ppm of manganese sulfate, 0.025ppm of zinc sulfate and 20kg of urea, uniformly stirring, and carrying out sealed fermentation at the conditions of pH5.5 and 30 ℃ for 240 hours to obtain a pure organic biological stock solution fertilizer rich in azotobacter and EM bacteria;
and step 3: adding 4kg of cytidine triphosphate aqueous solution into one ton of organic biological stock solution prepared by fermentation, adding 3kg of boric acid, 1.5kg of ammonium molybdate, 2.5kg of ferrous sulfate, 1.6kg of magnesium sulfate, 3kg of ammonium sulfate, 0.25kg of manganese sulfate, 0.01kg of zinc sulfate and 4.5kg of potassium dihydrogen sulfate, and fully and uniformly stirring to obtain the cytidine triphosphate biological organic liquid fertilizer.
Example 3:
the cytidine triphosphate biological organic liquid fertilizer formula comprises the following material components: 290kg of fish with squama, 11kg of high-activity rhizobium strain rich in azotobacter, 4kg of EM bacterial liquid, 5kg of trace elements, cytidine triphosphate aqueous solution and 21kg of urea.
In the invention, the trace elements comprise the following material components: 3kg of boric acid, 1.5kg of ammonium molybdate, 2.5kg of ferrous sulfate, 1.6kg of magnesium sulfate, 3kg of ammonium sulfate, 0.25kg of manganese sulfate, 0.01kg of zinc sulfate and 4.5kg of potassium dihydrogen sulfate.
The invention discloses a production method of a cytidine triphosphate biological organic liquid fertilizer formula, which is calculated by preparing one ton of cytidine triphosphate biological organic liquid fertilizer and comprises the following steps:
step 1: pulping prepared 300kg of fish with squama, adding 660kg of water, stirring uniformly, then adding ozone, after the fish paste is whitened and has no fishy smell, completing disinfection and sterilization, and standing the fish paste in a natural state until the ozone is volatilized;
step 2: adding 10kg of high-activity rhizobia strain rich in azotobacter, 5kg of EM bacterial liquid and 1.5kg of cytidine triphosphate aqueous solution, adding 0.4kg of ammonium molybdate, 0.25kg of magnesium sulfate, 0.3kg of ferrous sulfate, 0.4kg of ammonium sulfate, 0.15kg of monopotassium phosphate, 0.06kg of sodium chloride, 2.5ppm of manganese sulfate, 0.025ppm of zinc sulfate and 20kg of urea, uniformly stirring, and carrying out sealed fermentation at the conditions of pH5.5 and 30 ℃ for 240 hours to obtain a pure organic biological stock solution fertilizer rich in azotobacter and EM bacteria;
and step 3: adding 4kg of cytidine triphosphate aqueous solution into one ton of organic biological stock solution prepared by fermentation, adding 3kg of boric acid, 1.5kg of ammonium molybdate, 2.5kg of ferrous sulfate, 1.6kg of magnesium sulfate, 3kg of ammonium sulfate, 0.25kg of manganese sulfate, 0.01kg of zinc sulfate and 4.5kg of potassium dihydrogen sulfate, and fully and uniformly stirring to obtain the cytidine triphosphate biological organic liquid fertilizer.
Example 4:
the cytidine triphosphate biological organic liquid fertilizer formula comprises the following material components: 310kg of fish with squama, 9kg of high-activity rhizobia strain rich in azotobacter, 6kg of EM bacterial liquid, 6kg of trace elements, 6kg of cytidine triphosphate aqueous solution and 19kg of urea.
In the invention, the trace elements comprise the following material components: 3kg of boric acid, 1.5kg of ammonium molybdate, 2.5kg of ferrous sulfate, 1.6kg of magnesium sulfate, 3kg of ammonium sulfate, 0.25kg of manganese sulfate, 0.01kg of zinc sulfate and 4.5kg of potassium dihydrogen sulfate.
The invention discloses a production method of a cytidine triphosphate biological organic liquid fertilizer formula, which is calculated by preparing one ton of cytidine triphosphate biological organic liquid fertilizer and comprises the following steps:
step 1: pulping prepared 300kg of fish with squama, adding 660kg of water, stirring uniformly, then adding ozone, after the fish paste is whitened and has no fishy smell, completing disinfection and sterilization, and standing the fish paste in a natural state until the ozone is volatilized;
step 2: adding 10kg of high-activity rhizobia strain rich in azotobacter, 5kg of EM bacterial liquid and 1.5kg of cytidine triphosphate aqueous solution, adding 0.4kg of ammonium molybdate, 0.25kg of magnesium sulfate, 0.3kg of ferrous sulfate, 0.4kg of ammonium sulfate, 0.15kg of monopotassium phosphate, 0.06kg of sodium chloride, 2.5ppm of manganese sulfate, 0.025ppm of zinc sulfate and 20kg of urea, uniformly stirring, and carrying out sealed fermentation at the conditions of pH5.5 and 30 ℃ for 240 hours to obtain a pure organic biological stock solution fertilizer rich in azotobacter and EM bacteria;
and step 3: adding 4kg of cytidine triphosphate aqueous solution into one ton of organic biological stock solution prepared by fermentation, adding 3kg of boric acid, 1.5kg of ammonium molybdate, 2.5kg of ferrous sulfate, 1.6kg of magnesium sulfate, 3kg of ammonium sulfate, 0.25kg of manganese sulfate, 0.01kg of zinc sulfate and 4.5kg of potassium dihydrogen sulfate, and fully and uniformly stirring to obtain the cytidine triphosphate biological organic liquid fertilizer.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (8)

1. The cytidine triphosphate biological organic liquid fertilizer formula is characterized by comprising the following material components: 310kg of fish 290 with squama, 9-11kg of high-activity rhizobium strain rich in azotobacter, 4-6kg of EM bacterial liquid, 5-6kg of trace elements, cytidine triphosphate aqueous solution and 19-21kg of urea.
2. The cytidine triphosphate biological organic liquid fertilizer formula as claimed in claim 1, which comprises the following material components: 300kg of fish with squama, 10kg of high-activity rhizobium strain rich in azotobacter, 5kg of EM bacterial liquid, 5.5kg of trace elements, 5.5kg of cytidine triphosphate aqueous solution and 20kg of urea.
3. The cytidine triphosphate biological organic liquid fertilizer formula as claimed in claim 1, which comprises the following material components: 290kg of fish with squama, 11kg of high-activity rhizobium strain rich in azotobacter, 4kg of EM bacterial liquid, 5kg of trace elements, cytidine triphosphate aqueous solution and 21kg of urea.
4. The cytidine triphosphate biological organic liquid fertilizer formula as claimed in claim 1, which comprises the following material components: 310kg of fish with squama, 9kg of high-activity rhizobia strain rich in azotobacter, 6kg of EM bacterial liquid, 6kg of trace elements, 6kg of cytidine triphosphate aqueous solution and 19kg of urea.
5. The cytidine triphosphate biological organic liquid fertilizer formula as claimed in claim 1, wherein the trace elements comprise the following material components: 3kg of boric acid, 1.5kg of ammonium molybdate, 2.5kg of ferrous sulfate, 1.6kg of magnesium sulfate, 3kg of ammonium sulfate, 0.25kg of manganese sulfate, 0.01kg of zinc sulfate and 4.5kg of potassium dihydrogen sulfate.
6. A production method of a cytidine triphosphate biological organic liquid fertilizer formula is characterized by comprising the following steps of:
step 1: step 1: pulping prepared 300kg of fish with squama, and stirring uniformly;
step 2: filling 300kg of prepared fish paste into ozone for disinfection and sterilization, and completing disinfection and sterilization when the fish paste is whitened;
and step 3: 1kg of cytidine triphosphate aqueous solution, 10kg of high-activity rhizobium strain rich in azotobacter, 5kg of EM bacterial liquid, trace elements and 20kg of urea are sealed and fermented;
and 4, step 4: adding 4kg of cytidine triphosphate aqueous solution into one ton of organic biological stock solution prepared by fermentation, adding 3kg of boric acid, 1.5kg of ammonium molybdate, 2.5kg of ferrous sulfate, 1.6kg of magnesium sulfate, 3kg of ammonium sulfate, 0.25kg of manganese sulfate, 0.01kg of zinc sulfate and 4.5kg of potassium dihydrogen sulfate, and fully and uniformly stirring to obtain the cytidine triphosphate biological organic liquid fertilizer.
7. The method for producing cytidine triphosphate bio-organic liquid fertilizer formulation as claimed in claim 6, wherein in step 1, the fish paste is left to stand in a natural state until ozone volatilization is completed.
8. The method for producing the cytidine triphosphate biological organic liquid fertilizer formulation as claimed in claim 6, wherein the pure organic biological raw liquid fertilizer obtained in step 2 is hermetically fermented at 30 ℃ and pH5.5 for 240 hours.
CN202110708058.1A 2021-06-24 2021-06-24 Cytidine triphosphate biological organic liquid fertilizer formula and production method thereof Pending CN113416101A (en)

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1458130A (en) * 2002-05-17 2003-11-26 付责中 Process for producing organic biological composite fertilizer from biological wase material
CN103483093A (en) * 2013-10-09 2014-01-01 新疆高冠土壤研究所 Fluidic ribose alcohol nitrogen phosphor potassium fertilizer
CN103880482A (en) * 2014-03-21 2014-06-25 国农生物肥料连云港有限责任公司 Preparation method of crop organic fertilizer
CN105000993A (en) * 2015-07-23 2015-10-28 合肥众月健康科技有限公司 Liquid fertilizer with high permeability and preparation method thereof
CN106631558A (en) * 2016-11-15 2017-05-10 陈景河 Ecological environmental protection fish protein liquid fertilizer
CN108707563A (en) * 2018-05-28 2018-10-26 宁波吉丰生物科技发展有限公司 The preparation method of composite fermentation microbial inoculum and Fish protein amino acid Water soluble fertilizer
WO2019002634A1 (en) * 2017-06-27 2019-01-03 Fertinagro Biotech, S.L. Method for producing a yeast-based product with high nucleotide concentration

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1458130A (en) * 2002-05-17 2003-11-26 付责中 Process for producing organic biological composite fertilizer from biological wase material
CN103483093A (en) * 2013-10-09 2014-01-01 新疆高冠土壤研究所 Fluidic ribose alcohol nitrogen phosphor potassium fertilizer
CN103880482A (en) * 2014-03-21 2014-06-25 国农生物肥料连云港有限责任公司 Preparation method of crop organic fertilizer
CN105000993A (en) * 2015-07-23 2015-10-28 合肥众月健康科技有限公司 Liquid fertilizer with high permeability and preparation method thereof
CN106631558A (en) * 2016-11-15 2017-05-10 陈景河 Ecological environmental protection fish protein liquid fertilizer
WO2019002634A1 (en) * 2017-06-27 2019-01-03 Fertinagro Biotech, S.L. Method for producing a yeast-based product with high nucleotide concentration
CN108707563A (en) * 2018-05-28 2018-10-26 宁波吉丰生物科技发展有限公司 The preparation method of composite fermentation microbial inoculum and Fish protein amino acid Water soluble fertilizer

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