CN113414049B

CN113414049B - Intelligent atomizer and using method thereof

Info

Publication number: CN113414049B
Application number: CN202110625386.5A
Authority: CN
Inventors: 马伯宁; 戴平
Original assignee: St Thomas Advanced Materials
Current assignee: St Thomas Advanced Materials
Priority date: 2021-06-04
Filing date: 2021-06-04
Publication date: 2023-04-18
Anticipated expiration: 2041-06-04
Also published as: CN113414049A

Abstract

An intelligent atomizer and a using method thereof relate to the technical field of intelligent atomizers; the method comprises the following operation steps: s1: collecting and pretreating environmental particles; s2: carrying out atomization treatment; s3: inactivating blue light; s4: and (4) releasing. The intelligent atomizer in the prior art can not kill viruses enough to meet the current demand. The invention selects a wave band of 450nm as an irradiation source, reduces potential unsafe factors possibly brought by illumination, regulates irradiation intensity, keeps and improves immunogen performance of an irradiated object, selects a pretreatment mode of vitamin B2 sensitization, specifically uses a nuclear structure of a potential pathogen as an irradiation target point to inactivate the potential pathogen and lose infectivity, and then keeps the integrity of an outer membrane structure and antigen macromolecules such as glycoprotein embedded on a membrane as much as possible by reducing unit irradiation intensity.

Description

Intelligent atomizer and use method thereof

Technical Field

The invention relates to the technical field of intelligent atomizers, in particular to an intelligent atomizer and a using method thereof.

Background

Pathogens transmitted by respiratory tract, such as respiratory adenovirus transmitted between humans in spring and autumn, swine fever virus transmitted between animals, mycoplasma hyopneumoniae, influenza transmitted between humans and animals, coronavirus and the like enter the respiratory tract epithelial tissues of animals under the condition of complete vitality, often cause a series of contact respiratory tract infectious diseases, and are transmitted rapidly, and the incidence rate is often high. One of the more cost effective ways to control the prevention of this group of diseases is by spray immunization.

The literature reports that the method of spraying to prevent swine fever achieves new results. The swine fever is prevented by the generated aerosol through a spraying method. The technical route is originally derived from nasal spray immunization of SPF (specific pathogen free) chickens, so that the SPF chickens can obtain specific antibodies for laboratories to quickly and conveniently obtain antibody reagents. Further advances have been made to more practical use of this technology for large, medium and small animal mass immunization, thereby changing the traditional methods of capturing animals, immobilizing, and requiring individual intramuscular or subcutaneous injections. The practical problems of complexity, labor and time consumption and low efficiency are fundamentally solved. The work reported that 3500 fattening pigs were tested by using the hog cholera lapinized virus vaccine produced in China in two independent pastures in Jiangsu of China, and a satisfactory prevention effect was obtained. The method comprises the steps of sealing the pigsty to be used as an aerosol chamber, and spraying the vaccine for about 1 hour by adopting a special aerosol generator. The results confirmed that no detrimental health effects were observed, nor any damage to the porcine respiratory system was observed. The research shows that the spray immunity has good sensitivity and specificity. The vaccine can generate lasting and stable immunity after being sprayed with the hog cholera lapinized virus vaccine for 78 days, and can keep good protection for not less than 10 months.

For example, the commercial mycoplasma hyopneumoniae attenuated vaccine has a good immune effect on hybrid pigs, but the immune approach of intrapulmonary injection of the vaccine influences the wide popularization of the vaccine. The method has the advantages that large-scale prevention and control are tried by adopting an aerosol immunization mode, and the fact that the mode is a convenient and effective immunization mode is proved, and particularly, the spray immunization of attenuated or attenuated vaccines aiming at respiratory pathogens can play a direct and effective role. It has also been found that the activity of a vaccine attenuated to some extent will directly affect the immunoprotection effect of the vaccine. It is often considered that the shearing force generated by the spraying system during the atomization process will have a certain effect on the microbiological activity of the vaccine, and even 1 titer will be reduced, but the mycoplasma hyopneumoniae attenuated strain in the vaccine preparation under the inoculation condition can still be isolated, cultured, amplified and re-strengthened in the laboratory. Therefore, if safety priority is given, it is preferable to select a completely inactivated vaccine, although the attenuated vaccine, which has a stronger immune effect than the inactivated vaccine, can fully stimulate cellular immunity and humoral immunity of animals. However, if it is expected that the safest and earliest preventive and controlling effect of "natural immunity" can be obtained at the earliest initial stage in the case of spray immunization for large-scale population, or the case of dealing with new pathogens and variants with enhanced virulence, the principle that the mode of "inactivating virus" with the best safety should be considered most preferably. Even if it is not desired to sacrifice some immunostimulatory potency, it is desirable to consider the choice given the first established biological background of the pathogen.

The application proposes that pathogen particles in the air are subjected to hydration pretreatment and sensitization, then are subjected to atomization and blue light irradiation inactivation, and then are released back to the atmosphere, so that the treatment mode firstly ensures that the pathogen in the air is inactivated, the safety of a human body or an immune object is firstly guaranteed, and then the immunogen immune stimulation effect is played. The designed process not only ensures safety, but also can play a preposed preventive protection role particularly for unknown potential pathogens.

Compared to the smart atomizer method for spray immunization of the present application: the reported spray devices for spray immunization for immunization of raised economic animals focus on the control of the particle size of the spray particles or aerosols in order to increase the acceptance of immunogens by the respiratory tract as much as possible, for example aerosol particles of 10um size are a preferred one. In these cases, it is emphasized that the damage to pathogens, especially nucleic acid structures, by the high-frequency, high-power ultrasound is avoided as much as possible, preventing the excessive loss of activity of the vaccine for immunization placed in the smart nebulizer.

Due to the global spread of new crown epidemics, there has recently been much interest in how to prevent and control respiratory transmitted viruses. The Nausheen R.Shah of Bureaine State university issues and shows a design prototype of himself (N.R.Shah, I.Masic, C.Jonesand R.Gupta, personal ultrasound respiratory organ (purge) for complex-19. WSU-PHY-2001, 2002), aiming to provide a simple, cheap, easy-to-popularize, safe and effective technology and device for inactivating viruses in air. The efficacy of the LEDs with deep ultraviolet bands such as 220nm,222nm, 24584 nm,260nm,265nm and the like in inactivating viruses, which are verified by Shah of the inventor and refer to the experimental conditions of other researchers, the output irradiation power is distributed in the ranges of less than 2mJ/cm2,3.7mJ/cm2, 40mJ/cm2 and 50mJ/cm 2. It is particularly emphasized by the patent application that the inventive use of the device for single person air filtration purposes and in connection with various air treatment systems is of particular concern that toxic ozone and other by-products will not be generated in such a wavelength band, and inventor Shah has verified that ozone is not generated under 260nm irradiation and is therefore considered safe. In order to improve the irradiation efficiency, the inventor Shah further optimizes the structure of the air passage, the air passage material adopts UV reflecting material PTFE, the irradiation efficiency is improved by 10-50 times, the LED power supply is driven by a 12V battery which can provide a 500mA constant current source, and the LED is kept stable and durable in operation in a mode of air cooling and radiating fins. The final virus inactivation capacity can reach 99.99%, which is understood to reduce the virus titer by 4 units.

In order to realize safer virus inactivation technology, the clinically adopted inactivation technology of methylene blue or vitamin B2 is that the methylene blue with the concentration as low as 1uM and VB2 with the concentration of 10-200umol/L are added into a solution, so that the capability of targeted virus inactivation can be realized. And can even be used to process more fragile cultured cells. For example, when the riboflavin is added, combined with the cervical cancer cells normally cultured by 365nm ultraviolet irradiation and single 365nm ultraviolet irradiation, the morphological change of the cells is obviously different, and when the concentration of the Vb2 is lower, the riboflavin is mainly concentrated on the nuclear membrane and in the nucleus; when the concentration is higher, the distribution is also on the cell membrane, and the damage of the surrounding microstructure by the decomposition of the riboflavin under the illumination condition is very obvious. Compared with the method without adding Vb2 pure ultraviolet radiation, the targeting property is very obvious, and the required radiation dose is also reduced greatly.

Compared with methylene blue, riboflavin (vitamin B2) is a natural coenzyme with a wide range of functions, and can be used as a photosensitizer here, and together with FMN, FAD, riboflavin and derivative compounds participate in various redox processes in organisms. Riboflavin, FMN and FAD are easily degraded by light sources with wavelengths of 220nm,267nm,370nm,420nm to 560nm, and it is surprising that the quantum efficiency of riboflavin to the longer wavelength blue light portion is highest, and that riboflavin is excited by light to generate active oxygen molecules including superoxide anion and singlet oxygen, which cause guanine in nucleotide to be broken, and the DNA supercoiled structure of bacteria or viruses to be broken into open circles or lines. At present, photochemical technology of UV irradiating riboflavin tends to mature, which becomes effective and safe technology for killing microorganisms. In contrast, a 450nm light source is actually used as a radiation source for plant growth, and the radiation inactivation using a light source with a wave band near 450nm for medical use is mainly reported to be used for non-contact nondestructive treatment of blood plasma or whole blood products.

Compared with the existing or described intelligent atomizers, such as household intelligent atomizers or humidifiers, and traditional medical intelligent atomizers or humidifiers, the function of sterilizing and inactivating viruses is generally reserved, and the adopted broad-spectrum ultraviolet device is used for either direct irradiation for physical damage or sterilization or virus inactivation by means of free radicals with oxidation characteristics generated by a metal catalyst. Or to settle airborne particles by electrostatic adsorption. If the destruction of pathogens is particularly sought, the energy is generally increased by increasing the power of the radiation or by using radiation sources having as short a wavelength as possible. It is not assumed that an optimal combination of the interaction mechanisms of the combined factors, similar to that of the present application, is used to achieve a more specific solution beneficial to social security or to draw conclusions about which wavelength is chosen with technical advantages or in addition to other requirements through practical trial and screening.

Disclosure of Invention

The invention aims to solve the technical problem that the intelligent atomizer for killing viruses in the prior art is not satisfactory to meet the current requirements.

The purpose and the effect of the invention are achieved by the following specific technical means:

a method for using an intelligent atomizer comprises the following operation steps:

s1: collecting and treating environmental particles: trapping bacteria, mycoplasma, virus particles, etc. naturally existing in the air or artificially produced particles of pathogen, aerosol, etc. into an aqueous solution containing 10-200umol/L vitamin B2 by using air supply equipment;

s2: atomizing: dispersing the pretreated pathogen particles into tiny droplets in a fogging unit of the device, further shaping, and stripping and recovering a hydration layer;

s3: blue light inactivation: the liquid drops enter a blue light irradiation cavity with the size of 1-100um and the size of 420-460nm and stay for 1-20 seconds to receive the irradiation of an irradiation source 1.0-4000 mJ/Second;

s4: releasing: the irradiated pathogenic particles are released back to the gas phase of the environment to replace known or unknown virulent pathogens originally existing in the environment for stimulating the body to generate a controlled immune response.

Preferably, the concentration of particles derived from bacteria, mycoplasma, virus particles and the like or artificially produced pathogen particles, aerosol and the like in the air is increased by 2-6 orders of magnitude in the liquid phase and then the liquid phase is treated, and the concentration of the particles released from the outlet of the irradiation cavity is greater than that in the original air.

Preferably, the abundance of the 420-460nm wave band output by the blue light irradiation source is not less than 50% of the total irradiation energy, and the blue light irradiation source and the metal radiating fin assembled by heat conduction thereof are embedded in the heat conduction insulating glue and comprise a constant voltage source and a plurality of constant current branches which are connected in parallel and consist of constant current ICs or PTC thermosensitive current-limiting resistors.

Preferably, the parameters of the air supply amount of the air supply equipment, the atomizing amount of the atomizing unit and the unit irradiation energy of the blue light irradiation source are simultaneously controlled by a control system of a PWM output signal through preset coefficients.

Preferably, the control parameters of the control system include: the air supply speed of air supply equipment, the accumulated air-water mixing time, the ultrasonic spraying speed, the unit particle accumulated light radiation energy and the riboflavin concentration of the solution. These control parameters and combinations thereof are set with the aim of controlling the properties of the immunogenic activity of the released particles, in particular for different classes of pathogens requiring the use of an optimized combination of parameters.

The invention provides an intelligent atomizer which comprises a water tank with a water inlet pipe, an air supply device, a fogging unit and a spray inactivation unit, wherein the air distributor is arranged inside the water tank with the water inlet pipe, the air supply device comprises an interface for receiving an air source and an interface for outputting the air to the air distributor, the fogging unit is arranged at the upper end inside the water tank and comprises a liquid level track pipe, an ultrasonic vibrator, a venturi pipe and a liquid drop recovery sleeve pipe with a funnel opening at the top, the ultrasonic vibrator moving along with the change of the liquid level is arranged in the liquid level track pipe, the venturi pipe is arranged at the upper end of the ultrasonic vibrator in the liquid level track pipe, the liquid drop recovery sleeve pipe with the funnel opening at the top is sleeved at the upper end of the venturi pipe, the spray inactivation unit is arranged at the top of the fogging unit, a liquid drop recovery sheet is arranged between the fogging unit and the spray inactivation unit, the spray inactivation unit comprises a fixed seat, a reflecting pipe, a quartz transmission pipe and an LED array plate, a reflecting pipe is arranged at the upper end of the fixed seat, a quartz transmission pipe is arranged at the inner side of the fixed seat, the lower end of the quartz transmission pipe is communicated with the fogging unit, an LED plate, and an irradiation array cavity is arranged between the LED transmission pipe.

Preferably, an integrally formed heat dissipation substrate is disposed outside the LED array board.

Preferably, the liquid drop is retrieved piece center and is equipped with the square groove that runs through, runs through the inslot and is equipped with gas-liquid separation mechanism, and gas-liquid separation mechanism includes filter one, filter two and filter three, runs through the inslot portion and follows supreme filter one, filter two, filter three of being equipped with in proper order down, and the gas-liquid separation aperture of filter one, filter two, filter three is steadilyd decrease step by step, and the gas-liquid separation aperture of filter three is 20um.

Preferably, the left side and the right side of the corresponding second filter plate in the through groove are both provided with movable grooves, the inner sides of the movable grooves are smooth inclined planes, the inclined planes in the movable grooves are arranged in parallel, the left side and the right side of the second filter plate are both fixed with mounting lug plates, and the mounting lug plates are attached to one side, close to the inner parts of the movable grooves, of the movable grooves.

Preferably, be equipped with the cavity in the filter two, the lower extreme all is equipped with the filter tip that a plurality of groups extend to two outsides of filter in the cavity, the filter tip outside respectively with filter one, filter two meet mutually, be in fixed connection between the filter tip of the same row of cavity, and one side fixedly connected with spring that corresponds in filter tip and the cavity, during two initial position of filter, the spring that is located the cavity upper end is in normal condition, the spring that is located the cavity lower extreme is in the state of stretching out, the filter tip includes inlet end and filter end, inlet end and filter end integrated into one piece, the inlet end is equipped with the air inlet, the filter end both sides are equipped with the filter screen.

Advantageous effects

1. By using the device manufactured by the invention and the provided using method, the safety of the ambient air is obtained by inactivating known or unknown virus particles in an optimized mode; wherein in an alternative manner the concentration of the finally inactivated virus particles is increased by 2-6 orders of magnitude for acting as a natural immunogen and released back into the environment, which has the advantage that the nucleic acid structure of the passing pathogen particles is completely or partially destroyed, and the pathogen particles are finally inactivated and released back into the gas phase environment.

2. In the invention, a wave band of 450nm in a visible light wave band is selected as an irradiation source as much as possible, in order to further reduce potential unsafe factors brought by blue light to human eyes and further reduce irradiation intensity, but in order to improve the biological effect of irradiation, a vitamin B2 sensitization pretreatment mode is adopted, the nuclear structure of a potential pathogen is specifically used as an irradiation target point to inactivate the target point and lose infectivity, and then the integrity of antigen macromolecules such as an outer membrane structure and glycoprotein embedded on the membrane is kept as much as possible by reducing unit irradiation intensity.

3. The LED with 450nm is selected as the light source, so that the LED has remarkable advantages in the aspects of heat dissipation and photoelectric conversion efficiency, the requirement of a driving power supply can be greatly reduced, and the advantage obtained in economic aspects is also obvious enough by selecting the LED with 450nm as the light source.

4. The gas trapping part is opened in advance for 0.5 to 24 hours, and the gas is cleaned by the water solution in the water tank, so that potential pathogenic particles suspended in the air can be concentrated by 2000 to 200000 times; then the functions of spraying and blue light inactivation are started, and the titer of the inactivated pathogen particles can be reduced by 2-4 orders of magnitude.

Drawings

Fig. 1 is a flow chart of the method for using the intelligent atomizer of the invention.

Fig. 2 is a schematic front sectional view of the intelligent atomizer of the present invention.

Fig. 3 is a schematic view of the structure of the water tank of the present invention.

Fig. 4 is an exploded view of the fogging unit of the present invention.

Fig. 5 is a schematic top view of a spray inactivation unit of the present invention.

Fig. 6 is a schematic cross-sectional view of an LED array panel of the present invention.

FIG. 7 is a front sectional view of a droplet recovery sheet according to the present invention.

Fig. 8 is an enlarged view of the invention at a in fig. 7.

Figure 9 is a schematic diagram of a filter construction of the present invention.

In FIGS. 1-9: the device comprises a water tank 1, an air supply device 101, an air distributor 102, a water inlet pipe 103, a liquid level rail pipe 2, a limiting pipe groove 201, an ultrasonic vibrator 3, a venturi tube 4, a liquid drop recovery sleeve 5, a funnel opening 51, a liquid drop recovery sheet 6, a penetrating groove 61, a first filter plate 62, a second filter plate 63, a cavity 631, an installation ear plate 632, a filter 633, an adjusting hole 634, a third filter plate 64, a movable groove 65, a water dropping tank 66, a sliding rod 67, a spray inactivation unit 7, a fixed seat 8, a reflection pipe 9, a quartz transmission pipe 10, an LED array plate 11, an LED chip 111, a serial connection lead 112, an anode heat dissipation surface 113, a cathode heat dissipation surface 114, a cover plate 12, a limiting ear plate 13, a heat dissipation substrate 14, an irradiation cavity 15, an air inlet end 16, an air inlet 161, a filtering end 17 and a filtering net 171.

Detailed description of the preferred embodiments

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

Referring to fig. 1, a method for using an intelligent atomizer comprises the following operation steps:

s1: collecting and treating environmental particles: capturing and enriching bacteria, mycoplasma, virus particles and the like naturally existing in the air or artificially manufactured rigid particles such as pathogen particles and aerosol into an aqueous solution containing 10-200umol/L vitamin B2 by using air supply equipment;

s3: blue light inactivation: allowing the liquid drop to enter a blue light irradiation cavity with the size of 1-100um and the wavelength of 420-460nm for 1-20 seconds to receive the irradiation of a blue light irradiation source with the wavelength of 1.0-4000mJ/Second, preferably 450 nm;

s4: releasing: the irradiated liquid drops are released back to the environmental gas phase to replace known or unknown virulent pathogens existing in the environment, and the body is stimulated to generate a controlled immune response.

Wherein, the concentration of rigid particles such as bacteria, mycoplasma, virus particles and the like in the air or artificially produced pathogen particles, aerosol and the like is increased by 2-6 orders of magnitude before treatment, and the ratio of the gas phase concentration of the target pathogen particles finally released by the intelligent atomizer to the concentration before treatment is more than 2.0.

The abundance of 420-460nm wave band output by the blue light irradiation source is not less than 50% of total irradiation energy, preferably 450nm wave band, the blue light irradiation source and the metal radiating fin assembled by heat conduction thereof are embedded in the heat conduction insulating glue, and the blue light irradiation source comprises a constant voltage source and a plurality of constant current branches which are connected in parallel and consist of constant current ICs or PTC thermosensitive current limiting resistors.

The air supply amount of the air supply equipment, the atomizing amount of the atomizing unit and the unit irradiation energy parameter of the blue light irradiation source are controlled by a PWM output signal control system through preset coefficients.

Wherein, the rate parameter control vector for controlling the system to work correctly comprises: the air supply speed of air supply equipment, the accumulated air-water mixing time, the ultrasonic spraying speed, the unit particle accumulated light radiation energy and the riboflavin concentration of the solution.

The spray inactivation process and the air supply mixing can be controlled separately, one of the preferable modes is air supply mixing in advance, concentration of gas-phase particles is achieved, and the pathogenic particles in the air are captured and enriched to the aqueous storage solution and then are subjected to spray inactivation and discharge.

Wherein, the spray inactivation process and the air supply mixing can be coupled and carried out simultaneously, and one of the preferred modes is to spray, process and discharge after adding the pathogen sample needing to be processed into the water outlet tank.

The inactivation mode of specific pathogens or a class of pathogens can be optimized by setting a rate parameter control vector.

Wherein the final shape of the pathogenic particles released by the smart nebulizer is controlled by a rate parameter control vector, including attenuated but infectious particles, non-infectious but immunogenic particles.

Wherein, the infectious characters of the pathogenic particles finally released by the intelligent atomizer comprise a complete loss option and a partial retention option.

Wherein, the final option of the infectivity of the released particles is the result of optimization and combination setting of the control parameters.

Placing a 40W household intelligent atomizer in a room of 60 cubic meters for working, wherein the air supply modes comprise a rapid air supply mode and a spray air supply mode, the LED array plate 11 and the ultrasonic vibrator 3 are synchronously controlled together, and the specific power output control mode adopts a PWM (pulse width modulation) continuous manual knob control mode to ensure that the air supply quantity of a fan is continuously adjustable within 0.1-50CFM (continuous flow modulation), firstly, starting the fan to rapidly suck air in the environment, fully mixing particles suspended in the air and aqueous solution containing 50umol/L vitamin B2 by a 50um sintered air distributor 102 arranged in a 3L water storage tank 1 for 30-60 minutes, then starting a fogging unit and switching to the spray air supply mode, and pushing air mist or aerosol wrapped with active particles into a blue light tube with the length of 18 centimeters to be irradiated by air flow, and keeping the air flow in the blue light tube for about 2-4 seconds;

as shown in fig. 3, in the 450nm LED array plate loaded in the blue light irradiation cavity 15, continuously adjustable irradiation energy between 1mJ and 1500mJ/cm2 is projected onto aerosol or aerosol particles in the tube through the quartz tube 10, and then reflected by the reflection tube 9, and in the selection of other machine models, the quartz tube 10 can be replaced by an LED special-shaped lamp plate embedded by insulating silica gel;

an LED array plate 11 adopted on a household intelligent atomizer is a combination of 30lm/1W 450nm, LEDs and 3x3 chips carried by a 16mm aluminum substrate, a 350mA constant-current IC or PTC thermosensitive current-limiting resistor is arranged in front of each row, and the current-limiting resistors are connected with a 12cm case fan, the direct-current power supply of the part comes from a same 12V/1.2A constant-voltage output port, but a slightly different point is that a single manual switch is responsible for controlling the lighting and the extinguishing of the LED array plate or automatically controlling the selection of mode switching.

An industrial spray intelligent atomizer is arranged in a supermarket with the height of 50x20 meters and the height of 3.5 meters, the industrial spray intelligent atomizer comprises a central control unit and 30 sets of spray inactivation units which are dispersedly installed, the central control unit is communicated with the spray inactivation units through 485 serial ports, the power of a fresh air complete machine subjected to inactivation treatment is 3KW output through the spray inactivation units, the air supply modes comprise a night cruise air supply mode and a mute spray air supply mode, the air supply quantity of a unit fan is ensured to continuously work for 6-10 hours at 100-200CFM in the night cruise air supply mode, the fan quickly sucks air in the environment and sends the air into a 100L water tank 1, particles suspended in air are fully mixed with an aqueous solution containing 10umol/L vitamin B2 through a group of 50-micron series sintering air distributors 102, a spray blowing mode is switched to a spray blowing mode after a mute spray blowing mode is started, optionally dry air flow sent to a shelf area and wet air flow sent to a vegetable and flower area push aerosol or aerosol wrapped with active particles to 3-6 groups of 18-centimeter-length blue light tubes arranged in the units to be irradiated, the residence time in each blue light tube cavity is about 2-4 seconds, the blue light LED array plates 11 in each spray inactivation unit 7 adopt the same electrical parameters, and the blue light LED array plates are all embedded by insulating silica gel.

The chickens of all ages of the day can be infected with Newcastle disease, the susceptibility of the chicks is high, and the fatality rate of the non-immune chickens can reach more than 90 percent when infected; the source of infection of the newcastle disease virus is mainly sick chicken, and the transmission path is mainly a respiratory system, a digestive tract and eggs; randomly selecting 50 chickens in an independent chicken house from chicken houses in a private farm, collecting whole blood from a vein under a wing to prepare a serum sample, and simultaneously collecting cloacal cotton swabs for detecting Newcastle disease pathogens;

the hemagglutination and hemagglutination inhibition test flow used for serum sample antibody detection is carried out by adopting the national standard GB/T16550-2008: adding 25ul of normal saline into each first 12 wells of a 96-well hemagglutination plate; changing a suction head to suck 25ul of virus spraying liquid and adding the virus spraying liquid into the 1 st hole, taking 25ul of the uniformly mixed virus liquid from the 1 st hole and adding the virus liquid into the 2 nd hole, and sequentially diluting the 11 holes in this way; changing the suction head, and adding 25ul of physiological saline into each hole; changing pipette tips, and adding 25ul of 1% erythrocyte suspension into each hole 12; placing the reaction plate on a micro oscillator, oscillating and uniformly mixing, standing for 40min at room temperature (20-25 ℃) for observation, and judging the result; determining the typical button bottom sinking result of the red blood cells in the normal saline control holes, and further inclining the reaction plate to check the hemagglutination condition of the completely agglutinated virus liquid;

the specific process and operation of the hemagglutination inhibition test are as follows: adding 25ul of physiological saline into each hole of 1-11 holes, adding 25ul into the 12 th hole, sucking 25ul of serum to be detected, placing the serum into the 1 st hole, blowing and beating for 6-8 times, uniformly mixing the serum, sucking 25ul of the serum into the 2 nd hole, uniformly mixing the serum, sucking 25ul of the serum into the 3 rd hole, diluting the serum to the 10 th hole in a multiple ratio manner, sucking 25ul of the serum from the 10 th hole, discarding the serum, not adding the serum to be detected into the 11 th hole and the 12 th hole, using the 11 th hole as a virus control and the 12 th hole as a diluent control, changing a pipette tip, diluting standard positive serum and standard negative serum in the same manner, changing the pipette tip, sucking 4 diluted unit of virus liquid by using a micropipettor, adding the virus liquid into the 1-11 th holes, uniformly mixing the serum with 25ul of each hole, tapping a reaction plate, and standing at room temperature for 30min; changing pipette tips, respectively adding 25ul of 1% erythrocyte suspension into 1-12 holes, placing the reaction plate on a micro-oscillator, oscillating for 15-30 s, uniformly mixing, and standing at room temperature for 40min to determine the result.

Respectively adding 600ul of lysate into a sample for virus pathogen detection, a negative control and a positive control, fully and uniformly mixing the lysate and the lysate, and standing the mixture for 3 to 5min at room temperature; sucking the liquid into an adsorption column, and centrifuging at 13000r/min for 30s; discarding liquid in the collecting pipe, adding 600 mu L of washing liquid, and centrifuging at 13000r/min for 30s; the adsorption column was transferred to a new 1.5mL centrifuge tube and 50. Mu.L of the washing solution was added to the center of the column; standing at room temperature for 1min, and centrifuging at 13000r/min for 30s to collect virus RNA; taking 2ul of virus RNA extract for RT-PCR detection according to a national standard method, wherein the used conditions are as follows: 45min at 42 ℃; PCR procedure: 94 min; 94 35 cycles of 30s, 55 s and 72 30s, collecting reaction liquid, and observing the appearance of an amplification strip by electrophoresis;

detecting Newcastle disease antibodies and pathogens of 50 healthy chicks in an independent henhouse, wherein the detection result is that 96 percent of the serum antibody level of the chicks is less than 5log2, and the virus RNA detection of cotton swabs is negative;

extracting isotonic physiological saline extract of lung tissue, intestinal canal, feather and other tissues of 5 sick and dead chickens infected with Newcastle disease from a Newcastle disease area according to a national standard method, performing prefiltration, sterilizing, filtering, ultrafiltering and dehydrating to obtain 300ml of virus-containing sample concentrated solution, dividing into 100ml of 1 part and 3 parts in total, and freezing and storing at-20 ℃;

taking out 1 part of the virus suspension each time, adding 0.9% of physiological saline containing 0.05% of polyvinylpyrrolidone K30 buffer according to the proportion of 1;

a small high pressure aerosol generator was placed in an empty approximately 100 square meter chicken house and sprayed with a diluted virus spray at a rate of 5-10ml per minute into the room space. Simultaneously, a 100W industrial spray intelligent atomizer is started, a concentration mode is selected as a working mode, the air supply quantity of a fan is adjusted to be within 2.0CFM range for continuous working for 3-4 hours, 50umol/L vitamin B2 is added into a 5L water storage tank 1 of the atomizer in advance, 30-60 minutes after the spraying is finished, a generator and the intelligent atomizer are closed, the intelligent atomizer is moved to an independent henhouse to spray healthy chicks, an ultrasonic spray inactivation mode is started, the spraying speed is 200-500ml per hour, and the liquid in the 3L intelligent atomizer is sprayed completely;

repeating the above process every month, diluting 100ml of virus extract, and spraying for 1 day; detecting the immunity condition of 50 chicks to the Newcastle disease virus one month after spraying all 300ml of virus extracting solution;

the assay was 70% chicken serum antibody positive with 15 sample antibody levels less than 5log2, 14 sample antibodies 5log2, 10 sample antibody levels of 6log2,7 sample antibody levels equal to 7log2,3 sample antibody levels of 8log2,1 sample antibody level of 9log2, and no samples greater than 9log2 detected; the 362bp bands detected by the cotton swab virus RNA are all negative.

Referring to fig. 2-9, an intelligent atomizer, including water tank 1 that has inlet tube 103, air supply equipment 101, the unit of fogging and spraying deactivation unit 7, 1 inside fixed mounting of water tank that has inlet tube 103 has gas distributor 102, air supply equipment 101 is fixed in 1 outside of water tank, air supply equipment 101 is force fan, air supply equipment 101 is including the interface that receives the air supply and export the interface of gas distributor 102, 1 top of water tank is equipped with apron 12, the unit of fogging is installed to 1 inside upper end of water tank, the unit of fogging concrete structure is: the liquid level recovery device comprises a liquid level track pipe 2, an ultrasonic vibrator 3, a venturi tube 4 and a liquid drop recovery sleeve pipe 5 with a funnel opening 51 at the top, wherein the liquid level track pipe 2 is clamped and installed inside a water tank 1, a limiting pipe groove 201 is integrally formed around the outer side of the liquid level track pipe 2, limiting lug plates 13 matched with the limiting pipe groove 201 are fixed on the outer sides of the ultrasonic vibrator 3 and the venturi tube 4 respectively, the ultrasonic vibrator 3 is connected inside the liquid level track pipe 2 in a sliding mode, the ultrasonic vibrator 3 moves up and down along with the liquid level change in the liquid level track pipe 2, the venturi tube 4 is clamped and fixed at the upper end of the ultrasonic vibrator 3 inside the liquid level track pipe 2, the liquid drop recovery sleeve pipe 5 with the funnel opening 51 at the top is sleeved on the upper end of the venturi tube 4, the top of the atomizing unit is provided with an atomizing inactivation unit 7, a liquid drop recovery sheet 6 is arranged between the atomizing unit and the atomizing inactivation unit 7, the atomizing inactivation unit 7 comprises a fixed seat 8, a reflection tube 9, a quartz transmission tube 10 and an LED array plate 11, a limiting lug plate 13 corresponding to the limiting tube groove 201 is fixed on the outer side of the fixed seat 8, the reflection tube 9 is fixed on the upper end of the fixed seat 8, the quartz transmission tube 10 is fixed on the inner side of the reflection tube 9 on the upper end of the fixed seat 8, the lower end of the quartz transmission tube 10 is communicated with the inside of the liquid level rail tube 2, the LED array plate 11 is installed on the outer side of the quartz transmission tube 10 on the fixed seat 8, and an irradiation cavity 15 is arranged between the LED array plate 11 and the quartz transmission tube 10; the air in the environment is sucked by a pressure fan, particles suspended in the air are fully mixed with an aqueous solution containing vitamin B2 in a water tank 1 by an air distributor 102, then the position of the particles in a liquid level rail pipe 2 is moved up and down along with the change of the liquid level by an ultrasonic wave 3 vibrator, the aqueous solution is made into a liquid with the size of 1-100um, the liquid drops are pushed by airflow to pass through a venturi tube 4 and then pass through a liquid drop recovery sheet 6, redundant liquid drops fall onto a liquid drop recovery sleeve 5 with a funnel opening 51 at the top, the liquid drops are discharged into the water tank 1 through the funnel opening 51, the suspended rigid particles wrapped by the liquid drops with smaller particle size recombination are pushed under the action of the airflow to be irradiated by 450nm in a quartz transmission tube 10 in a spray inactivation unit 7, and stay in the quartz transmission tube 10 for 1-10 seconds and then are released into the environment again to be suspended in a stable aerosol mode, the irradiation energy with 450nm is projected onto aerosol or aerosol particles in the quartz transmission tube 10, and then the aerosol or aerosol particles are reflected back to be irradiated repeatedly by a mirror reflection tube 14.

The outer side of the LED array plate 11 is provided with an integrally formed heat dissipation substrate 14, and the heat of the 450nm LED is dissipated through the heat dissipation substrate 14 in a natural heat dissipation manner to keep the intelligent atomizer in a long-term running state.

The LED array board 11 comprises a plurality of LED patch chips 111, the LED patch chips 111 are connected in series through a series lead 112 to form a group to work in a constant current limiting mode, the positions of an anode radiating surface 113 and a cathode radiating surface 114 are anode and cathode radiating surfaces which are independently designed according to specific parameters of the LED patch chips 11, sufficient radiating efficiency and thermoelectric separation effect are guaranteed, the positions of the LED patch chips 111 are 1-3W high-power 450nm and LED chips of a patch, and a plane structure without a condensing mirror surface and with an emission angle of 120 degrees is selected under a common condition.

Wherein, the liquid drop is retrieved 6 centers and is equipped with square through groove 61, is equipped with gas-liquid separation mechanism in the through groove 61, and gas-liquid separation mechanism specific structure is: including filter one 62, filter two 63 and filter three 64, run through inside from supreme filter one 62 that is equipped with in proper order down of groove 61, filter two 63, filter three 64, filter one 62, filter two 63, the gas-liquid separation aperture of filter three 64 progressively diminishes, the gas-liquid separation aperture of filter three 64 is 20um, the effect that obtains is that unnecessary liquid drop or particle diameter exceed 20 um's liquid drop and retrieve again and get back to in the liquid pool.

The left side and the right side of the inside of the penetrating groove 61, which correspond to the second filter plate 63, are provided with movable grooves 65, the inner sides of the movable grooves 65 are provided with smooth inclined surfaces, the inclined surfaces inside the movable grooves 65 are arranged in parallel, the left side and the right side of the second filter plate 63 are respectively fixed with a mounting lug plate 632, one side, which is close to the inside of the movable groove 65, of the mounting lug plates 632 is jointed with the inclined surfaces inside the movable grooves 65, vertical slide rods 67 are fixed in the movable grooves 65, the upper ends of the mounting lug plates 623 are provided with strip-shaped adjusting holes 634, the adjusting holes 634 are sleeved at the upper ends of the slide rods 67, and the bottom of the movable groove 65 is provided with a water falling groove 66 communicated with the adjusting holes 634; the air flow speed at the outlet of the venturi 4 is accelerated, the second filter plate 63 floats along the movable groove 65, the inner side of the movable groove 65 is obliquely arranged, and the second filter plate 63 deviates from the left and right positions in the floating process and can better contact with vaporized liquid drops.

Wherein, a cavity 631 is arranged in the second filter plate 63, a plurality of groups of filter tips 633 extending to the outer side of the second filter plate 63 are arranged at the upper end and the lower end in the cavity 631, the filter tips 633 move towards the inner side of the cavity 631 under the extrusion force, the outer side of the filter tips 633 is respectively connected with the first filter plate 62 and the second filter plate 63, the filter tips 633 in the same row of the cavity 631 are fixedly connected with each other, a spring is fixedly connected with one corresponding side of the filter tips 633 in the cavity 631, when the second filter plate 63 is at the initial position, the spring at the upper end of the cavity is in a normal state, the spring at the lower end of the cavity is in an extended state, the filter tips 633 comprise an air inlet end 16 and a filter end 17, the air inlet end 16 and the filter end 17 are integrally formed, an air inlet 161 is arranged at the air inlet end 16, and filter screens 171 are arranged at the two sides of the filter end 17; two 63 filter in-process at the rising, the filter 633 that is located the upper end then receives the atress and contracts toward cavity 631 inside, but laminating filter three 64 all the time, filter 633 that is located the lower extreme then stretches out cavity 631 by the spring action, also can contact with one 62 filter all the time, skew about two 63 rises the emergence of filter simultaneously, make filter 633 can scrape the liquid drop that attaches two 63 surface impurity of filter 62 and filter or drippage, reach the cleaning action, filter 633 then can reach automatically cleaning filter screen 171 at the in-process of cavity 631 internal contraction.

The air in the environment is sucked by a pressure fan, the particles suspended in the air are fully mixed with the water solution containing vitamin B2 in the water tank 1 by an air distributor 102, then the ultrasonic vibrator 3 is utilized, the position of the inner part of the liquid level rail pipe 2 moves up and down along with the change of the liquid level to prepare the water solution into liquid with the size of 1-100um, the liquid drops are pushed by air flow to pass through a venturi 4 and pass through a liquid drop recovery sheet 6, the air flow speed at the outlet of the venturi 4 is accelerated, a second filter plate 63 floats along a movable groove 65, the inner side of the movable groove 65 is obliquely arranged, so that the left and right position of the second filter plate 63 is deviated in the floating process, the filter tip 633 at the upper end of the second filter plate 63 is stressed to contract towards the inner part of the cavity 631 in the rising process, but is always attached to the third filter tip 64 at the lower end, and extends out of the cavity 631 under the action of a spring, the filter tip 633 can scrape impurities or drops which do not drop on the surfaces of the first filter plate 62 and the second filter plate 63 to achieve a cleaning effect, the filter tip 633 can achieve a self-cleaning filter screen 171 in the process of extending in the cavity 631, the drops pass through the first filter plate 62, the second filter plate 63 and the third filter plate 64, redundant drops fall onto the drop recovery sleeve 5 with the funnel 51 at the top through the water falling groove 66, the drops are discharged into the water tank 1 through the funnel 51, the drops with the reduced particle size recombination are wrapped by suspended rigid particles, are sent to the quartz transmission tube 10 in the spray inactivation unit 7 under the action of air flow to be irradiated by 450nm, stay in the quartz transmission tube 10 for 1-10 seconds and are released to the environment again to be suspended in a stable aerosol mode, the 450nm radiation energy is transmitted through the quartz transmission tube 10 and projected onto the aerosol or aerosol particles in the tube, and then reflected back through the specular reflection tube 14 to repeat the radiation.

Claims

1. The intelligent atomizer comprises a water tank (1) with a water inlet pipe (103), an air supply device (101), a fogging unit and a spray inactivation unit (7), and is characterized in that the water tank (1) with the water inlet pipe (103) is internally provided with an air distributor (102), the air supply device (101) comprises an interface for receiving an air source and an interface for outputting the air distributor (102), the fogging unit is installed at the upper end inside the water tank (1), the fogging unit comprises a liquid level track pipe (2), an ultrasonic vibrator (3), a venturi tube (4) and a liquid drop recovery sleeve (5) with a funnel opening (51) at the top, the ultrasonic vibrator (3) is arranged in the liquid level track pipe (2) and moves along with the change of the liquid level, the upper end of the ultrasonic vibrator (3) in the liquid level track pipe (2) is provided with the venturi tube (4), the upper end of the venturi tube (4) is provided with the liquid drop recovery sleeve (5) with the funnel opening (51) at the top, the top of the fogging unit is provided with the spray inactivation unit (7), the spray inactivation unit (7) is provided with a spray inactivation array plate (9), and a reflection plate (9), the upper end of the fixed seat (8) is positioned on the inner side of the reflecting tube (9) and is provided with a quartz transmission tube (10), the lower end of the quartz transmission tube (10) is communicated with the fogging unit, the outer side of the quartz transmission tube (10) is provided with an LED array plate (11), an irradiation cavity (15) is arranged between the LED array plate (11) and the quartz transmission tube (10), the center of the liquid drop recovery sheet (6) is provided with a square through groove (61), a gas-liquid separation mechanism is arranged in the through groove (61) and comprises a first filter plate (62), a second filter plate (63) and a third filter plate (64), the inside of the through groove (61) is sequentially provided with the first filter plate (62), the second filter plate (63) and the third filter plate (64) from bottom to top, the gas-liquid separation apertures of the first filter plate (62), the second filter plate (63) and the third filter plate (64) are gradually reduced, and the gas-liquid separation aperture of the third filter plate (64) is 20um; the filter comprises a first filter plate (62) and a second filter plate (63), wherein a movable groove (65) is formed in the left side and the right side of the penetrating groove (61) corresponding to the second filter plate (63), the inner side of the movable groove (65) is a smooth inclined surface, the inclined surfaces in the movable groove (65) are arranged in parallel, mounting lug plates (632) are fixed on the left side and the right side of the second filter plate (63), one side, close to the movable groove (65), of each mounting lug plate (632) is attached to the inclined surface in the movable groove (65), a cavity (631) is formed in the second filter plate (63), a plurality of groups of filter tips (633) extending to the outer side of the second filter plate (63) are arranged at the upper end and the lower end of the cavity (631), the outer side of each filter tip (633) is respectively connected with the first filter plate (62) and the second filter tip (63), the cavity (631) is fixedly connected with the filter tips (633) in a row, springs at the upper end of the cavity (631) and one side corresponding to the cavity (631), when the filter tip (63) is in the initial position, the springs at the upper end of the cavity (63), the cavity are in a normal state, the springs at the lower end of the cavity, the cavity (633) are located at the end of the filter tips (16) and at the two sides of the filter tip (17), and the filter tip (17) are integrally formed by the filter tip (17), and the filter tip (16).

2. The intelligent atomizer according to claim 1, characterized in that, the outside of the LED array plate (11) is provided with an integrally formed heat dissipation substrate (14).