CN113388507B - Nucleic acid extraction and PCR detection all-in-one machine - Google Patents
Nucleic acid extraction and PCR detection all-in-one machine Download PDFInfo
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- CN113388507B CN113388507B CN202110643507.9A CN202110643507A CN113388507B CN 113388507 B CN113388507 B CN 113388507B CN 202110643507 A CN202110643507 A CN 202110643507A CN 113388507 B CN113388507 B CN 113388507B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D46/00—Filters or filtering processes specially modified for separating dispersed particles from gases or vapours
- B01D46/10—Particle separators, e.g. dust precipitators, using filter plates, sheets or pads having plane surfaces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
Abstract
The invention provides a nucleic acid extraction and PCR detection integrated machine, which comprises a machine shell, a nucleic acid extraction module and a PCR detection module, wherein the machine shell is a hollow shell, the nucleic acid extraction module and the PCR detection module are arranged inside the machine shell, an air inlet and an air outlet are formed in the machine shell, a first filter is arranged on the air inlet, a second filter is arranged at the air outlet, a wind shield is arranged at the position, close to the air inlet, of the inner side of the machine shell, the wind shield is arranged between the air inlet and the PCR detection module, and the wind shield extends from the air inlet to the nucleic acid extraction module; the bottom of the nucleic acid extraction module is provided with a first air duct, the bottom of the PCR detection module is provided with a second air duct, and the first air duct is communicated with the second air duct; and one side of the fan, which is far away from the diversion air channel, is communicated with the second filter. The invention has the advantages of preventing the nucleic acid extraction module and the PCR detection module from being polluted by outside air and avoiding cross contamination.
Description
Technical Field
The invention relates to the technical field of polymerase chain reaction technical equipment, in particular to a nucleic acid extraction and PCR detection integrated machine.
Background
Document CN110331090A discloses a nucleic acid extraction, amplification and detection device, which includes a base, an extraction component and an amplification detection component, wherein the extraction component includes a reagent rack detachably mounted on the base, and a liquid suction mechanism for sucking a reagent, the reagent rack includes a first body, and a reaction cavity and a plurality of reagent cavities arranged on the first body, the reagent cavities respectively contain an extraction reagent and a reaction reagent, and the liquid suction mechanism respectively sucks a sample, the extraction reagent and the reaction reagent and transfers the sucked sample and reagent into the reaction cavity; the amplification detection assembly comprises an amplification detection chamber, and the amplification detection chamber is arranged on the base; the amplification detection chamber contains a reaction cavity for amplifying, detecting and analyzing the extracted sample. The reagent rack of the device can be detached and replaced, and different samples can be analyzed quickly; the operation process of nucleic acid extraction and the structure of the extraction assembly are simplified, and the cost of the device and the operation is reduced; the integrated arrangement is adopted, the structure is compact, the volume is small, and the carrying is convenient. However, the above technical solution still requires to manually place the reaction cavity in the accommodating portion of the amplification detection chamber, which easily causes reagent contamination and affects the PCR experimental result.
CN208472055U full-automatic nucleic acid extraction amplification diagnosis all-in-one, including the base and set up the support on the base, still including: a nucleic acid extraction module; a sample access mechanism; a mechanical arm mechanism; a hardware installation module; the PCR module is used for detecting a sample, and is arranged on one side of the hardware installation module and positioned below the bracket; a medical waste storage box; the reagent storage mechanism is used for storing reagents and is arranged above the medical waste storage box; and the reaction cup sample introduction mechanism is arranged at the front end of the nucleic acid extraction module and is positioned on the base. By adopting the technical scheme, all the components are integrated on the base to form the whole set of equipment, the structure is integrated, the streamlined operation is realized, the test time is short, and the using effect is good; the hardware installation module integrates all circuit boards in the hardware module, so that the problem of external electromagnetic interference is avoided, data fluctuation caused by electromagnetic interference is avoided, and the test precision is high. However, in the above-mentioned technical solution, the nucleic acid extraction module and the PCR module are disposed at different positions of the rack, and no isolation or airflow path is provided, which easily causes cross contamination between the nucleic acid extraction module and the PCR reagent due to aerosol escape.
Disclosure of Invention
Aiming at the problem that cross contamination is easy to occur to reagents in the prior art, the invention provides an integrated machine for nucleic acid extraction and PCR detection.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows: a nucleic acid extraction and PCR detection integrated machine comprises a machine shell, a nucleic acid extraction module and a PCR detection module, wherein the machine shell is a hollow shell, the nucleic acid extraction module and the PCR detection module are arranged in the machine shell, an air inlet and an air outlet are formed in the machine shell, a first filter is arranged at the air inlet, a second filter is arranged at the air outlet, a wind shield is arranged at a position, close to the air inlet, on the inner side of the machine shell, the wind shield is arranged between the air inlet and the PCR detection module, and the wind shield extends from the air inlet to the nucleic acid extraction module;
the bottom of the nucleic acid extraction module is provided with a first air duct, the bottom of the PCR detection module is provided with a second air duct, and the first air duct is communicated with the second air duct; and one side of the second air duct, which is far away from the first air duct, is sequentially provided with a turning air duct and a fan, and one side of the fan, which is far away from the turning air duct, is communicated with a second filter.
The PCR detection module is a closed cavity, and one side of the PCR detection module, which is close to the nucleic acid extraction module, is provided with a lifting door.
The second filter is a HEPA filter screen.
The second filter is a V-shaped densely-pleated filter.
An air receiving opening is arranged between the first air duct and the second air duct.
The air inlets are mesh holes arranged in an array.
A first temperature controller is arranged on the inner side of the shell close to the air inlet, and a second temperature controller is arranged on the inner side of the first air duct.
The first temperature controller and the second temperature controller are both Peltier elements.
The first temperature controller is provided with first radiating fins, and the second temperature controller is provided with second radiating fins.
A first temperature sensor is arranged in the machine shell and close to the nucleic acid extraction module, and a second temperature sensor is arranged on the inner side of the first air channel.
The invention has the following beneficial effects: the first filter is arranged at the air inlet, so that the nucleic acid extraction module and the PCR detection module are prevented from being polluted by external air; through setting up deep bead, first wind channel, second wind channel, diversion wind channel, make gaseous at first through nucleic acid extraction module, pass through PCR detection module afterwards to prevent that the aerosol of PCR result from polluting nucleic acid extraction module, avoid cross contamination.
Drawings
FIG. 1 is a schematic diagram of an external structure of an embodiment of an integrated machine for nucleic acid extraction and PCR detection.
FIG. 2 is a schematic diagram of an internal structure of an embodiment of an integrated machine for nucleic acid extraction and PCR detection.
FIG. 3 is a schematic diagram of the internal structure of another embodiment of the integrated machine for nucleic acid extraction and PCR detection.
In the figure: 1. a housing; 2. a nucleic acid extraction module; 3. a PCR detection module; 4. a lift gate; 5. an air inlet; 6. an air outlet; 7. a first filter; 8. a first air duct; 9. a second air duct; 10. a diversion air duct; 11. a fan; 12. a second filter; 13. an air collecting opening; 14. a first temperature controller; 15. a first heat radiation fin; 16. a second temperature controller; 17. a second heat radiation fin; 18. a first temperature sensor; 19. a second temperature sensor; 20. a wind deflector.
Detailed Description
The invention will be further described with reference to the following detailed description of the drawings.
It should be noted that, without conflict, any combination of the various embodiments or technical features described below may form a new embodiment.
It is to be understood that the terms "center," "upper," "lower," "horizontal," "left," "right," "lateral," "longitudinal," and the like are used in the orientations and positional relationships indicated in the drawings for the convenience of describing the present invention and for the simplicity of description, and do not indicate or imply that the referenced devices or elements must be in a particular orientation, constructed and operated in a particular orientation, and thus are not to be considered as limiting the present invention.
The first embodiment is as follows:
as shown in fig. 1 and 2, an integrated machine for nucleic acid extraction and PCR detection comprises a casing 1, a nucleic acid extraction module 2, and a PCR detection module 3, wherein the casing 1 is a hollow casing, the nucleic acid extraction module 2 and the PCR detection module 3 are disposed inside the casing 1, an air inlet 5 and an air outlet 6 are disposed on the casing 1, the air inlet 5 is provided with a first filter 7, the air outlet 6 is provided with a second filter 12, a wind shield 20 is disposed at a position close to the air inlet 5 inside the casing 1, the wind shield 20 is disposed between the air inlet 5 and the PCR detection module 3, and the wind shield 20 extends from the air inlet 5 to the nucleic acid extraction module 2;
the bottom of the nucleic acid extraction module 2 is provided with a first air duct 8, the bottom of the PCR detection module 3 is provided with a second air duct 9, and the first air duct 8 is communicated with the second air duct 9; the side, far away from the first air duct 8, of the second air duct 9 is sequentially provided with a turning air duct 10 and a fan 11, and the side, far away from the turning air duct 10, of the fan 11 is communicated with a second filter 12.
Description of the principles of the invention: under the action of the fan 11, air in the machine shell 1 enters from the air inlet 5, is filtered by the first filter 7, then passes through the nucleic acid extraction module 2, the first air duct 8, the second air duct 9 and the turning air duct 10 respectively, then passes through the PCR detection module 3, and is filtered by the second filter 12, and then is discharged out of the machine shell 1 from the air outlet 6, so that the air flow in the machine shell 1 flows.
The first filter 7 is arranged at the air inlet 5 to filter the air entering the machine shell 1, so that the nucleic acid extraction module 2 and the PCR detection module 3 are prevented from being polluted by the outside air; through setting up deep bead 20, first wind channel 8, second wind channel 9, diversion wind channel 10, make gaseous at first through nucleic acid extraction module 2, pass through PCR detection module 3 afterwards to prevent the aerosol pollution nucleic acid extraction module 2 of PCR result, avoid cross contamination.
As shown in fig. 2, the PCR detection module 3 is a sealed cavity, and a lifting door 4 is disposed on one side of the PCR detection module 3 close to the nucleic acid extraction module 2. Whether the cavity of the PCR detection module 3 is closed or not is controlled by the lifting door 4, and the lifting door 4 is closed when the PCR operation is carried out so as to prevent the PCR reagent from being polluted by the gas in the nucleic acid extraction module 2 and other positions. Further, the lift gate 4 is in a normally closed state.
The second filter 12 is a HEPA screen. Through using the HEPA filter screen, filter the gas that flows out casing 1, prevent that the gas in the casing 1 from polluting the external environment.
The second filter 12 is a V-type dense-pleated filter to filter the gas while securing the flow rate of the gas.
As shown in fig. 2, an air receiving opening 13 is disposed between the first air duct 8 and the second air duct 9, so as to collect the airflow of the first air duct 8 into the second air duct 9.
As shown in fig. 1, the air inlets 5 are mesh holes arranged in an array. The net-shaped holes arranged in the array can be used for preliminarily filtering the floating objects in the air, so that the first filter 7 is prevented from being blocked.
Example two:
as shown in fig. 3, on the basis of the first embodiment, a first temperature controller 14 is disposed inside the casing 1 near the air inlet 5, and a second temperature controller 16 is disposed inside the first air duct 8. The gas temperature near the nucleic acid extraction module 2 is adjusted by the first temperature controller 14, and the gas temperature near the PCR detection module 3 is controlled by the second temperature controller 16, so that the gas pressure difference around the nucleic acid extraction module 2 and the PCR detection module 3 is adjusted, the gas pressure near the nucleic acid extraction module 2 is higher than the gas pressure near the PCR detection module 3, the gas flows naturally in the direction from the nucleic acid extraction module 2 to the PCR detection module 3 in the casing 1, and the aerosol generated by the reagent of the PCR detection module 3 is prevented from being dissipated to the vicinity of the nucleic acid extraction module 2 to pollute the nucleic acid extraction module 2. Because the cavity of the PCR detection module 3 is controlled to be in a closed state by the lifting door 4, the airflow flowing from the nucleic acid extraction module 2 to the PCR detection module 3 cannot enter the PCR detection module 3, thereby preventing the pollution of the reagent in the PCR detection module 3.
The first temperature controller 14 and the second temperature controller 16 are both peltier elements. By using peltier elements as the first temperature controller 14 and the second temperature controller 16, the temperature of the respective temperature controllers can be controlled by controlling the current to perform cooling or heating, thereby facilitating temperature control.
As shown in fig. 3, the first temperature controller 14 is provided with first heat radiating fins 15, and the second temperature controller 16 is provided with second heat radiating fins 17. The temperature adjusting effect of the corresponding temperature controller on the ambient gas is improved through the radiating fins.
As shown in FIG. 3, a first temperature sensor 18 is disposed in the casing 1, the first temperature sensor 18 is close to the nucleic acid extraction module 2, and a second temperature sensor 19 is disposed inside the first air duct 8. The detection of the gas temperature around the nucleic acid extraction module 2 by the first temperature sensor 18, in combination with the first temperature controller 14, prevents the gas temperature from affecting the nucleic acid extraction operation, while facilitating the calculation of the gas pressure in the vicinity of the nucleic acid extraction module 2. The temperature of the gas in the first air duct 8 is detected by the second temperature sensor 19, so that the air pressure near the PCR detection module 3 can be calculated conveniently, and the temperature near the PCR detection module 3 can be controlled by the second temperature controller 16.
Finally, it should be noted that the above-mentioned contents are only used for illustrating the technical solutions of the present invention, and not for limiting the protection scope of the present invention, and that the simple modifications or equivalent substitutions of the technical solutions of the present invention by those of ordinary skill in the art can be made without departing from the spirit and scope of the technical solutions of the present invention.
Claims (9)
1. The utility model provides a nucleic acid extraction and PCR detect all-in-one, includes casing, nucleic acid extraction module, PCR detection module, its characterized in that: the casing is a hollow casing, the nucleic acid extraction module and the PCR detection module are arranged in the casing, an air inlet and an air outlet are arranged on the casing, a first filter is arranged at the air inlet, a second filter is arranged at the air outlet, a wind shield is arranged at a position, close to the air inlet, on the inner side of the casing, the wind shield is arranged between the air inlet and the PCR detection module, and the wind shield extends from the air inlet to the nucleic acid extraction module;
the bottom of the nucleic acid extraction module is provided with a first air duct, the bottom of the PCR detection module is provided with a second air duct, and the first air duct is communicated with the second air duct; a turning air channel and a fan are sequentially arranged on one side, far away from the first air channel, of the second air channel, and one side, far away from the turning air channel, of the fan is communicated with a second filter;
the PCR detection module is a closed cavity, and one side of the PCR detection module, which is close to the nucleic acid extraction module, is provided with a lifting door.
2. The integrated nucleic acid extraction and PCR detection machine of claim 1, wherein: the second filter is a HEPA filter screen.
3. The integrated nucleic acid extraction and PCR detection machine of claim 2, wherein: the second filter is a V-shaped densely-pleated filter.
4. The integrated nucleic acid extraction and PCR detection machine of claim 1, wherein: an air receiving opening is arranged between the first air duct and the second air duct.
5. The integrated nucleic acid extraction and PCR detection machine of claim 1, wherein: the air inlets are mesh holes arranged in an array.
6. The integrated nucleic acid extraction and PCR detection machine of claim 1, wherein: a first temperature controller is arranged on the inner side of the shell close to the air inlet, and a second temperature controller is arranged on the inner side of the first air duct.
7. The integrated nucleic acid extraction and PCR detection machine of claim 6, wherein: the first temperature controller and the second temperature controller are both Peltier elements.
8. The integrated nucleic acid extraction and PCR detection machine of claim 6, wherein: the first temperature controller is provided with first radiating fins, and the second temperature controller is provided with second radiating fins.
9. The integrated nucleic acid extraction and PCR detection machine of claim 6, wherein: a first temperature sensor is arranged in the machine shell and close to the nucleic acid extraction module, and a second temperature sensor is arranged on the inner side of the first air channel.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110643507.9A CN113388507B (en) | 2021-06-09 | 2021-06-09 | Nucleic acid extraction and PCR detection all-in-one machine |
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| CN202110643507.9A CN113388507B (en) | 2021-06-09 | 2021-06-09 | Nucleic acid extraction and PCR detection all-in-one machine |
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| CN113388507A CN113388507A (en) | 2021-09-14 |
| CN113388507B true CN113388507B (en) | 2022-02-25 |
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| CN115747025B (en) * | 2022-10-14 | 2023-09-22 | 北京卓诚惠生生物科技股份有限公司 | Full-automatic nucleic acid extraction and PCR detection all-in-one |
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| FR2939445B1 (en) * | 2008-12-10 | 2013-05-03 | Biomerieux Sa | AUTOMATED LYSE SYSTEM OF MICROORGANISMS PRESENT IN SAMPLE, NUCLEIC ACID EXTRACTION AND PURIFICATION OF MICROORGANISMS FOR ANALYSIS |
| TWI480377B (en) * | 2013-04-02 | 2015-04-11 | Genereach Biotechnology Corp | Methods for detecting Bacillus anthracis |
| CN206751853U (en) * | 2017-05-01 | 2017-12-15 | 任碧琼 | A kind of full-automatic nucleic acid extraction, amplification integrated apparatus |
| CN207016784U (en) * | 2017-07-31 | 2018-02-16 | 华东医药（杭州）基因科技有限公司 | A kind of environmental control system |
| CN107760676B (en) * | 2017-11-14 | 2020-06-23 | 孔治 | Full-automatic extraction instrument for paramagnetic particles |
| CN209652319U (en) * | 2018-09-11 | 2019-11-19 | 青岛海华生物医药技术有限公司 | A kind of vent cabinet and pig circular ring virus PCR sensing chamber |
| CN110940818A (en) * | 2018-09-25 | 2020-03-31 | 绍兴普施康生物科技有限公司 | Chemiluminescence detection equipment and operation method thereof |
| CN110951610B (en) * | 2019-12-31 | 2023-05-05 | 苏州缔因安生物科技有限公司 | Full-integrated miniaturized chip type digital PCR detection system and detection method |
| CN111672858A (en) * | 2020-07-03 | 2020-09-18 | 上海百傲科技股份有限公司 | Cabinet type air exhaust PCR device |
| CN112899151A (en) * | 2021-02-01 | 2021-06-04 | 青岛迪诺瓦基因科技有限公司 | Flowing liquid temperature changing device and using method thereof |
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