CN112899151A - Flowing liquid temperature changing device and using method thereof - Google Patents
Flowing liquid temperature changing device and using method thereof Download PDFInfo
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- CN112899151A CN112899151A CN202110133189.1A CN202110133189A CN112899151A CN 112899151 A CN112899151 A CN 112899151A CN 202110133189 A CN202110133189 A CN 202110133189A CN 112899151 A CN112899151 A CN 112899151A
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- bladder
- flowing liquid
- liquid temperature
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
- B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Abstract
The invention discloses a flowing liquid temperature changing device and a using method thereof, wherein the flowing liquid temperature changing device comprises a flowing pipe, two ends of the flowing pipe are closed, and one end of the flowing pipe is provided with an air pressure component; a sample inlet opening is arranged on the flow pipe, and a sealing plug is arranged in the sample inlet opening; the flow pipe is provided with at least two temperature areas, and the temperature difference exists between the temperatures of the temperature areas; the temperature change speed of the liquid in the pipe can be increased, and the detection work requirement is met.
Description
Technical Field
The invention belongs to the technical field of pathogen detection, and particularly relates to a flowing liquid temperature changing device and a using method thereof.
Background
At present, in nucleic acid gene detection such as pathogen detection, prenatal detection, genetic disease screening, tumor screening and the like, a fluorescent quantitative detection mode is required, wherein temperature-variable amplification is the most accurate and common mode, and the temperature-variable amplification of taq enzyme is realized under the condition that a sample is subjected to temperature variation at two different temperatures. The existing temperature-variable amplification PCR instrument (including a quantitative PCR instrument) mostly has the problems of low temperature rise and fall speed, long amplification time and the like, and the temperature rise is probably 15 seconds, the temperature fall is 15 seconds, so that the detection time of the whole sample is overlong at present, and is about 30min-2.5 h.
Disclosure of Invention
The invention provides a flowing liquid temperature changing device and a using method thereof, which are used for solving the problem that the temperature changing device used for fluorescence quantitative detection in the prior art has longer temperature rising and temperature reducing time.
The invention provides a flowing liquid temperature changing device, which comprises a flow pipe, wherein two ends of the flow pipe are closed, and one end of the flow pipe is provided with an air pressure assembly; a sample inlet opening is arranged on the flow pipe, and a sealing plug is arranged in the sample inlet opening; the flow tube is provided with at least two temperature zones, and the temperature of each temperature zone has a temperature difference.
Further, the air pressure component is an air bag or an injector.
Further, the air pressure component is an air bag, and the air bag is an elastic air bag.
Further, the air pressure assembly is an air bag, and the air bag is a flat elastic buffer bag.
Further, the other end of the flow pipe is also provided with the pneumatic assembly.
Furthermore, the air bag at one end of the flow tube is an elastic air bag, and the air bag at the other end of the flow tube is a flat elastic buffer bag.
Furthermore, a self-locking cover is arranged on the plugging plug.
The invention also provides a using method of the flowing liquid temperature changing device, which comprises the following steps,
opening a sealing plug on the flow tube, injecting a sample through the sample injection opening to enable the sample to enter the flow tube, and then sealing the sample injection opening by the sealing plug;
heating or cooling each temperature area so that the sample is heated or cooled in each temperature area;
and starting the air pressure assembly to compress air, pushing the heated or refrigerated sample in the flow pipe to flow, and realizing the temperature change of the sample flowing between the temperature areas.
More specifically, the air pressure assembly is set as an air bag, and when the air pressure assembly is started, air is compressed by compressing the air bag; or the air pressure component is set as an injector, and when the air pressure component is opened, air is compressed by pushing a piston push rod of the injector.
More specifically, a support surface is arranged on the flow pipe, and the flowing liquid temperature changing device is placed and installed through the support surface.
The invention has the beneficial effects that:
the flowing liquid temperature changing device and the use method thereof can solve the problem that the temperature changing device used for fluorescence quantitative detection in the prior art has longer temperature rising and temperature reducing time, can improve the temperature changing speed of liquid in the pipe, and meet the detection work requirement.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention and not to limit the invention. In the drawings:
fig. 1 is a schematic structural view of a flowing liquid temperature change device of the present invention.
In the figure, 1 is a flow tube, 2 is a sealing plug, 3 is an elastic air bag, 4 is an elastic buffer bag, 5 is a self-locking cover, and 6 is a supporting surface.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be clearly and completely described below with reference to the specific embodiments of the present invention and the accompanying drawings. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The technical solutions provided by the embodiments of the present invention are described in detail below with reference to the accompanying drawings.
Example 1
Referring to fig. 1, a flowing liquid temperature changing device comprises a flow pipe 1, wherein both ends of the flow pipe 1 are closed, and one end of the flow pipe 1 is provided with an air pressure assembly; a sample inlet opening is arranged on the flow pipe 1, and a sealing plug 2 is arranged in the sample inlet opening; the flow tube 1 is provided with at least two temperature zones, the temperature of each of which has a temperature difference.
In the flowing liquid temperature changing device of the present embodiment, the flow tube 1 is used for storing liquid, and the inner diameter of the flow tube is smooth, so that the liquid can move back and forth left and right in the flow tube 1. The pneumatic assembly can compress air to provide pressure to drive the liquid in the flow pipe 1 to move left and right. The air pressure component is connected with the flow pipe 1 in a sealing way, so that air leakage or liquid leakage caused by overlarge internal pressure is prevented. The closed end of the flow tube 1 is also protected against gas or liquid leakage. The sealing plug 2 is used for sealing the sample inlet opening.
Example 2
A flowing liquid temperature change device, similar to example 1, except that the air pressure component is a bladder or syringe.
Or, the air pressure component is an air bag, and the air bag is an elastic air bag 3.
Or, the air pressure component is an air bag which is a flat elastic buffer bag 4.
Example 3
A flowing liquid temperature changing device, similar to embodiment 1 or embodiment 2, except that the other end of the flow tube 1 is also provided with said air pressure assembly.
Furthermore, the air bag at one end of the flow tube 1 is an elastic air bag 3, and the air bag at the other end is a flat elastic buffer bag 4.
Further, the sealing plug 2 is provided with a self-locking cover 5. So as to prevent the air leakage of the flow tube or the leakage of the contained liquid and prevent the seal plug 2 from automatically falling off.
Example 4
A flowing liquid temperature change device, similar to any of examples 1-3, except that the number of temperature zones is two, including a high temperature zone and a low temperature zone.
Further, the temperature of the high-temperature area is 90-98 ℃.
Further, the temperature of the high-temperature area is 93-96 ℃.
Further, the temperature of the low-temperature area is 55-72 ℃.
Further, the temperature of the low-temperature region is 59-63 ℃.
Example 5
A flowing liquid temperature change device similar to any one of embodiments 1-4 except that the flexible bladder 3 is circular or elliptical.
Further, the self-locking cover 5 is a cover plate with a clamping groove on the bottom surface, and the clamping groove is clamped on the outer wall of the flow pipe 1.
Furthermore, a support surface 6 is provided on the flow tube 1.
Further, the support surface 6 is a plane.
Further, the number of the support surfaces 2 is two, and the two support surfaces are respectively positioned above and below the flow tube 1.
Furthermore, the support surface 2 above the flow tube 1 is located above the support surface 1 below the flow tube 1.
Further, the flow tube 1 is a circular tube.
Further, the flow tube 1 has an inner diameter of 1 to 5 mm.
Further, the flow tube 1 has an inner diameter of 2 to 2.5 mm.
It should be noted that, if the inner diameter of the flow tube 1 is too large, the use requirement cannot be met, and if the inner diameter is too small, the mold is too thin, and the mold opening cannot be performed.
Further, a support surface 6 is provided on the flow tube 1 on the side of the elastic bladder 3.
Further, a sealing plug 2 is disposed on the flow tube 1 on the side of the elastic buffer bladder 4.
Furthermore, a temperature control sheet is arranged at the temperature area.
Further, the temperature control sheet is an electric heating sheet.
Furthermore, the temperature control chip is an electric refrigeration chip.
Furthermore, the temperature control sheet is an electric heating sheet with bidirectional current, and the electric heating sheet is heated when forward current is introduced and is refrigerated when reverse current is introduced; or the electric heating sheet refrigerates when being electrified with reverse current and heats when being electrified with the reverse current.
Further, the flow pipe 1 is a rigid plastic pipe, a PP pipe, polyethylene, polyvinyl chloride, polyvinyl fluoride, or the like.
Further, the sealing plug 2 is a silica gel plug or a rubber plug.
Furthermore, the air bag is made of elastic materials such as a rubber bag or a silicon bag.
Example 6
The method of using a flowing liquid temperature change device of any one of embodiments 1 through 5, comprising the steps of,
opening a sealing plug 2 on a flow tube 1, injecting a sample through the sample inlet opening to enable the sample to enter the flow tube 1, and then sealing the sample inlet opening by the sealing plug 2;
heating or cooling each temperature area so that the sample is heated or cooled in each temperature area;
and starting the air pressure assembly to compress air to push the heated or refrigerated sample in the flow tube 1 to flow, so as to realize the temperature change of the sample flowing between the temperature areas.
Example 7
The method of using a flowing liquid temperature change device of embodiment 6, more specifically, providing the air pressure component as an air cell, and compressing air by compressing the air cell when the air pressure component is activated; or the air pressure component is set as an injector, and when the air pressure component is opened, air is compressed by pushing a piston push rod of the injector.
More specifically, a support surface 6 is arranged on the flow pipe 1, and the flowing liquid temperature changing device is placed and installed through the support surface 6.
The above description is only an example of the present application and is not intended to limit the present application. Various modifications and changes may occur to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the scope of the claims of the present application.
Claims (10)
1. The flowing liquid temperature changing device comprises a flow pipe and is characterized in that two ends of the flow pipe are closed, and one end of the flow pipe is provided with an air pressure assembly; a sample inlet opening is arranged on the flow pipe, and a sealing plug is arranged in the sample inlet opening; the flow tube is provided with at least two temperature zones, and the temperature of each temperature zone has a temperature difference.
2. The flowing liquid temperature change device of claim 1, wherein the air pressure component is a bladder or a syringe.
3. The flowing liquid temperature change device of claim 1, wherein said air pressure component is an air bladder, said air bladder being an elastomeric air bladder.
4. The flowing liquid temperature change device of claim 1, wherein said air pressure component is an air bladder, said air bladder being a flat, resilient, cushioning bladder.
5. The flowing liquid temperature change device of claim 1, wherein the other end of the flow tube is also provided with said air pressure assembly.
6. The flowing liquid temperature change device of claim 5, wherein said bladder at one end of the flow tube is an elastomeric bladder and said bladder at the other end is a flat elastomeric cushion bladder.
7. The flowing liquid temperature change device of claim 1, wherein the plug is provided with a self-locking cap.
8. The method of using a flowing liquid temperature change device of any one of claims 1-7, comprising the steps of,
opening a sealing plug on the flow tube, injecting a sample through the sample injection opening to enable the sample to enter the flow tube, and then sealing the sample injection opening by the sealing plug;
heating or cooling each temperature area so that the sample is heated or cooled in each temperature area;
and starting the air pressure assembly to compress air, pushing the heated or refrigerated sample in the flow pipe to flow, and realizing the temperature change of the sample flowing between the temperature areas.
9. The method of using a flowing liquid temperature change device of claim 8 wherein said air pressure assembly is configured as a bladder, and wherein said air pressure assembly is activated to compress air by compressing said bladder; or the air pressure component is set as an injector, and when the air pressure component is opened, air is compressed by pushing a piston push rod of the injector.
10. The method of using a flowing liquid temperature change device of claim 8, wherein said flowing tube is provided with a support surface by which said flowing liquid temperature change device is placed in installation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110133189.1A CN112899151A (en) | 2021-02-01 | 2021-02-01 | Flowing liquid temperature changing device and using method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110133189.1A CN112899151A (en) | 2021-02-01 | 2021-02-01 | Flowing liquid temperature changing device and using method thereof |
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| CN112899151A true CN112899151A (en) | 2021-06-04 |
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| CN202110133189.1A Pending CN112899151A (en) | 2021-02-01 | 2021-02-01 | Flowing liquid temperature changing device and using method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113388507A (en) * | 2021-06-09 | 2021-09-14 | 北京卓诚惠生生物科技股份有限公司 | Nucleic acid extraction and PCR detection all-in-one machine |
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