CN202766523U - Polymerase chain reaction (PCR) low-temperature dedicated loading ice box - Google Patents
Polymerase chain reaction (PCR) low-temperature dedicated loading ice box Download PDFInfo
- Publication number
- CN202766523U CN202766523U CN 201220500978 CN201220500978U CN202766523U CN 202766523 U CN202766523 U CN 202766523U CN 201220500978 CN201220500978 CN 201220500978 CN 201220500978 U CN201220500978 U CN 201220500978U CN 202766523 U CN202766523 U CN 202766523U
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- sample
- application
- pcr
- special
- low temperature
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Abstract
The utility model relates to a PCR low-temperature dedicated loading ice box. The ice box comprises a supporting box and a protective cover matched with the supporting box to be used. A loading frame is connected with a clamping groove at the top of the supporting box, a transverse plate is arranged at the lower end of the loading frame and fixed with the circumference of the inner wall of the supporting box to form a sealed cavity, and refrigerants are filled in the sealed cavity. The PCR low-temperature dedicated loading ice box is fixed and sealed, so that the advantage of convenience and fastness is reflected in three aspects of carrying, usage and management; the transverse plate with U-shaped grooves are arranged in the supporting box, so that a PCR tube is placed under a low-temperature condition in a loading process, the phenomenon of water vapor condensation is avoided simultaneously, and the accuracy of experimental results is guaranteed; and a support plate is added in the supporting box, so that the firmness of the supporting box is further improved, and the service life is prolonged.
Description
Technical field
The utility model relates to the medical experiment apparatus, what be specifically related to is the special-purpose application of sample ice chest of a kind of PCR low temperature.
Background technology
PCR, Chinese are the polymerase chain reaction.Round pcr is the common method of the synthetic specific DNA fragment of a kind of external enzymatic.
Because at normal temperatures, primer can react under the effect of enzyme, mispairing occurs produce wrong PCR product, affects test-results.Therefore, carry out under the environment that is lower than melting temperature(Tm) of being everlasting in the PCR test, this moment, the reactive behavior of enzyme can reduce, thereby avoided non-specific amplification, and ensured result's accuracy.
At present, adding below the model ice bag on the pad for the general method of PCR low temperature application of sample, this method ice bag that operates dissolves the full operator's console that the liquid that comes can flow and all is, must clean operator's console after the test, and is very inconvenient.
In addition, a kind of PCR ice chest is arranged also, it is that ice bag is placed in the holder box, holder box top is put temporarily and is added model, although this mode can solve the problem that liquid that ice bag melts out flows everywhere, the setting that disperses between all parts is not easy to the equipment control of testing laboratory.Simultaneously, owing in the PCR ice chest parts that anti-steam condenses not being set, often be that the PCR pipe is in the ice bath fully, cause in the process of application of sample, airborne water molecules can condense on the inwall of PCR pipe, and then has increased the content of moisture content in the PCR reaction, meanwhile, the water molecules that condenses in the air has affected the accuracy of PCR reaction result toward the material that there is dust granule in the contact meeting and suppresses the PCR reaction.
The utility model content
Goal of the invention of the present utility model is to provide a kind of PCR low temperature special-purpose application of sample ice chest for the problems referred to above, be used for solving ice bag and have awkward problem, and there is the parts scattering device in the PCR ice chest that uses at present, is not easy to manage and easily occur the problem that condensation vapor affects test-results.
The utility model is achieved through the following technical solutions: the special-purpose application of sample ice chest of PCR low temperature comprise the holder box and with the matching used shield cap of holder box, described holder box top draw-in groove is connected with the application of sample frame, application of sample frame lower end is provided with transverse slat, transverse slat is circumferentially fixing with holder box inwall, form airtight chamber, be filled with refrigeration agent in the airtight chamber.
As further improvement of the utility model, be provided with Label clip on the described shield cap.
As further improvement of the utility model, be provided with the solution expansion pipe on the described holder box sidewall.
As further improvement of the utility model, be provided with pressure cap on the described solution expansion pipe.
As further improvement of the utility model, described holder box bottom is provided with back up pad.
As further improvement of the utility model, described application of sample frame is 24 commonly used hole application of sample framves, in the 96 hole application of sample framves any one.
As further improvement of the utility model, described refrigeration agent is sodium polyacrylate solution.
As further improvement of the utility model, be provided with on the described transverse slat and the matching used U-shaped groove of application of sample frame.
As further improvement of the utility model, described U-shaped groove is the groove of height in 0.25 to 1 cm range.
Adopt the positively effect of technique scheme:
1, the utility model is set to the ice chest of a fixing seal, all embodies easily advantage carrying, use, manage three broad aspect;
2, the utility model arranges the transverse slat with U-shaped groove in the holder box, makes in the application of sample process, and the PCR pipe is under the low-temperature condition, has avoided again the phenomenon of condensation vapor simultaneously, has guaranteed the accuracy of test-results;
3, the utility model has further improved the stability of holder box in the back up pad that holder adds in the box, has prolonged work-ing life.
Description of drawings
Fig. 1 is structural representation of the present utility model.
Among the figure: 1 holder box, 2 shield caps, 3 Label clips, 4 application of sample framves, 5 airtight chamber, 6 transverse slats, 7 solution expansion pipes, 8 back up pads, 9 pressure caps.
Embodiment
For technique means, creation characteristic that the utility model is realized, reach purpose and effect is easy to understand, below in conjunction with concrete diagram, further set forth the utility model.
As shown in Figure 1, the special-purpose application of sample ice chest of PCR low temperature comprise holder box 1 and with holder box 1 matching used shield cap 2.The setting of shield cap 2 can effectively prolong the temperature control time.For convenient record test coding or label, be difficult for occuring the situation of testing data confusion simultaneously, can Label clip 3 be set at shield cap 2.
Top at holder box 1 is provided with application of sample frame 4, hold in the palm simultaneously that draw-in groove is connected between box 1 and the application of sample frame 4, can take off application of sample frame 4 like this when freezing holder box 1, purpose is the situation that occurs condensation vapor in order to reduce variation owing to application of sample frame 4 temperature and to cause testing in the PCR pipe.
Be provided with transverse slat 6 in application of sample frame 4 lower ends, and transverse slat 6 is circumferentially fixing with holder box inwall, forms airtight chamber 5, is filled with refrigeration agent in the airtight chamber 5.During use, refrigeration agent specifically can be set to sodium polyacrylate solution, also can select according to practical situation other refrigeration agent.For can be in the application of sample process, the PCR pipe both be under the low-temperature condition, can avoid better again the phenomenon of condensation vapor simultaneously, and warranty test result's accuracy can arrange and application of sample frame 4 matching used U-shaped grooves on the transverse slat 6.If what application of sample frame 4 was selected is 24 hole application of sample framves, on that transverse slat 6 also correspondence 24 U-shaped grooves are set; If what application of sample frame 4 was selected is 96 hole application of sample framves, on that transverse slat 6 also correspondence 96 U-shaped grooves are set.
In addition, according to the experience of test, the height of U-shaped groove is arranged in 0.25 to 1 centimetre the scope, the effect that anti-steam condenses is better.
In the utility model, for the increase that prevents the material generation volume in the freezing rear airtight chamber 5 causes holding in the palm box 1 to deform or damages, can be provided with solution expansion pipe 7 at holder box 1 sidewall.Simultaneously, fall in the airtight chamber 5 in order to prevent foreign matter, also can add pressure cap 9 at solution expansion pipe 7.Opening also can be to holder box 1 interior additional sodium polyacrylate solution behind the pressure cap 9.
In the molecular biology test chamber, the correlation test amount of PCR is very large, for increasing wearing quality and whole stability, can back up pad 8 be set in the bottom of holder box 1.
Application of sample frame 4 described in the utility model is 24 hole application of sample framves commonly used, in the 96 hole application of sample framves any one.And the thickness of 24 hole application of sample framves, 96 hole application of sample framves is arranged on 0.4 to 0.8 centimetre is advisable.
Above demonstration and described ultimate principle of the present utility model, principal character and advantage of the present utility model.The technician of the industry should understand; the utility model is not restricted to the described embodiments; that describes in above-described embodiment and the specification sheets just illustrates principle of the present utility model; under the prerequisite that does not break away from the utility model spirit and scope; the utility model also has various changes and modifications, and these changes and improvements all fall in claimed the utility model scope.The claimed scope of the utility model is defined by appended claims and equivalent thereof.
Claims (9)
1. the special-purpose application of sample ice chest of a PCR low temperature, comprise the holder box (1) and with the holder the matching used shield cap of box (1) (2), it is characterized in that: described holder box (1) top draw-in groove is connected with application of sample frame (4), application of sample frame (4) lower end is provided with transverse slat (6), transverse slat (6) is circumferentially fixing with holder box inwall, form airtight chamber (5), airtight chamber is filled with refrigeration agent in (5).
2. the special-purpose application of sample ice chest of the PCR low temperature shown in according to claim 1 is characterized in that: be provided with Label clip (3) on the described shield cap (2).
3. the special-purpose application of sample ice chest of the PCR low temperature shown in according to claim 1 is characterized in that: be provided with solution expansion pipe (7) on described holder box (1) sidewall.
4. the special-purpose application of sample ice chest of the PCR low temperature shown in according to claim 3 is characterized in that: be provided with pressure cap (9) on the described solution expansion pipe (7).
5. the special-purpose application of sample ice chest of the PCR low temperature shown in according to claim 1, it is characterized in that: described holder box (1) bottom is provided with back up pad (8).
6. the special-purpose application of sample ice chest of the PCR low temperature shown in according to claim 1 is characterized in that: described application of sample frame (4) is 24 hole application of sample framves commonly used, in the 96 hole application of sample framves any one.
7. the special-purpose application of sample ice chest of the PCR low temperature shown in according to claim 1, it is characterized in that: described refrigeration agent is sodium polyacrylate solution.
8. the special-purpose application of sample ice chest of the PCR low temperature shown in according to claim 1 is characterized in that: be provided with on the described transverse slat (6) and the matching used U-shaped groove of application of sample frame (4).
9. the special-purpose application of sample ice chest of the PCR low temperature shown in according to claim 8, it is characterized in that: described U-shaped groove is the groove of height in 0.25 to 1 cm range.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220500978 CN202766523U (en) | 2012-09-28 | 2012-09-28 | Polymerase chain reaction (PCR) low-temperature dedicated loading ice box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220500978 CN202766523U (en) | 2012-09-28 | 2012-09-28 | Polymerase chain reaction (PCR) low-temperature dedicated loading ice box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202766523U true CN202766523U (en) | 2013-03-06 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220500978 Expired - Fee Related CN202766523U (en) | 2012-09-28 | 2012-09-28 | Polymerase chain reaction (PCR) low-temperature dedicated loading ice box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202766523U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103740582A (en) * | 2014-01-17 | 2014-04-23 | 俞冬熠 | PCR (Polymerase Chain Reaction) sampling ice box |
-
2012
- 2012-09-28 CN CN 201220500978 patent/CN202766523U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103740582A (en) * | 2014-01-17 | 2014-04-23 | 俞冬熠 | PCR (Polymerase Chain Reaction) sampling ice box |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130306 Termination date: 20130928 |