CN102876569A - Capillary tube for heat convection polymerase chain reaction device - Google Patents
Capillary tube for heat convection polymerase chain reaction device Download PDFInfo
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- CN102876569A CN102876569A CN2011101984771A CN201110198477A CN102876569A CN 102876569 A CN102876569 A CN 102876569A CN 2011101984771 A CN2011101984771 A CN 2011101984771A CN 201110198477 A CN201110198477 A CN 201110198477A CN 102876569 A CN102876569 A CN 102876569A
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- polymerase chain
- chain reaction
- reaction device
- kapillary
- thermal convection
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Abstract
The invention provides a capillary tube for a heat convection polymerase chain reaction device. The capillary tube is arranged on a test tube seat and comprises a tube body, wherein the tube body is provided with long tube-shaped accommodation space which is provided with an axial section; and the distance between the left and right edges of the axial section is greater than the distance between the front and rear edges of the axial section. When a reaction sample is filled into the long tube-shaped accommodation space and is heated to produce heat convection, the left and right edges of the axial section can form a relatively narrow channel for reducing the flow velocity of the rise of the heat convection, the circulation time of the heat convection can be prolonged, and the reaction time of the reaction sample can be prolonged, so the reaction efficiency of the heat convection polymerase chain reaction device can be increased.
Description
Technical field
The present invention relates to a kind of thermal convection polymerase chain reaction device, relate in particular to a kind of kapillary for thermal convection polymerase chain reaction device.
Background technology
Take polymerase chain reaction (PCR) amplification specific nucleic acid sequence as at present suitable proven technique, it is the important technology of medical science and biotechnology, its reaction process mainly contains three steps: reaction and extension are closed in reaction of degeneration, refining, and wherein the temperature of reaction of these three steps needs is not identical.Business-like PCR equipment now, required reaction sample comprise the wish amplification template DNA, with each chain of template DNA on the oligonucleotide introduction of particular sequence complementation to, heat-resistant quality archaeal dna polymerase and deoxidation ribonucleoside triphosphote (dNTP).PCR equipment circulates the reaction sample, with the specific part of amplification template DNA nucleotide sequence by repeatedly heating and cooling reaction sample between three kinds of differing tempss.
First step of PCR is reaction of degeneration, and it is heated to high temperature for reacting sample, and to allow double-stranded template DNA be separated into single stranded DNA, typically the temperature of reaction of degeneration is the scope between 90 ℃ to 95 ℃.
The PCR second step is that chain closes reaction, it is cooled to lesser temps for the reaction sample that will be separated into first single stranded DNA, to allow introduction be combined with the single stranded DNA that first step forms, and the mixture of formation DNA and introduction, typically the refining temperature of closing reaction is selected for the melting temperature(Tm) (melting temperature:Tm) of the used introduction of foundation, usually between 35 to 65 ℃ scope.
The 3rd step of PCR is polymerization (extension) reaction, it is that the reaction sample that will form the mixture of DNA and introduction is maintained at proper temperature, effect by archaeal dna polymerase, introduction is extended, the new single stranded DNA of formation and each chain complementation of template DNA, the temperature of typical polymerization reaction is 72 ℃.
Therefore the each time circulation that is formed by above-mentioned three steps, can copy the template DNA of twice, to comprise reaction of degeneration, chain and close the PCR of the different step of three temperature such as reaction and extension and be cycled to repeat approximately 20 to 40 times, can produce the replica of millions of target nucleotide sequences.
In traditional commercially available PCR instrument (being the thermal cycler device), the temperature of reaction sample is to control with heat exchange pattern.The reaction vessel of PCR reaction sample is housed for to contact with the solid metal block with high thermal conductivity.This metal block links to each other with heating and refrigerating unit, and changes temperature by heating and refrigerating unit, and traditional thermal cycling PCR need spend extra time and the energy and go to heat and cool off material beyond the PCR sample itself.And because the accurate characteristic of machine itself, so the thermal cycling instrument is usually very expensive.
See also Fig. 1 and shown in Figure 2, another technology than the cost-saving is thermal convection PCR (CPCR) at present, the reaction sample of thermal convection PCR commonly used is to be packed in the circular kapillary 1, a plurality of kapillary 1 proper alignment are equiped with on the test tube flat 2 of well heater (not shown) and refrigerating unit (not shown) in one, the reaction sample that is wherein driven by thermal convection, by the effect of well heater and refrigerating unit, circulate in this kapillary 1 interior formation.Therefore the different temperature of kapillary 1 each regional tool is reacted sample at each interregional circulation time and work as, and three steps of PCR can sequentially and repeatedly occur.
Summary of the invention
Therefore, main purpose of the present invention is to disclose a kind of capillary pipe structure, can prolong the cycling time of thermal convection.
Via as can be known above, for reaching above-mentioned purpose, the invention provides a kind of kapillary for thermal convection polymerase chain reaction device, for being arranged on the test tube flat, it comprises a body, and this body has an elongated tubular accommodation space, this elongated tubular accommodation space axially and the plane of this test tube flat angle that tilts, and this elongated tubular accommodation space tool one axial cross section, the distance of the left and right edges of this axial cross section is greater than the distance at the edge, front and back of this axial cross section.
Kapillary for thermal convection polymerase chain reaction device of the present invention, wherein, this axial cross section is occluding surface, and the curvature of this axial cross section left and right edges is greater than the curvature at edge before and after this axial cross section.
Kapillary for thermal convection polymerase chain reaction device of the present invention, wherein, this axial cross section is elliptical area.
Kapillary for thermal convection polymerase chain reaction device of the present invention, wherein, this axial cross section is rectangle.
Kapillary for thermal convection polymerase chain reaction device of the present invention, wherein, this body encloses around a high heat conduction ring in the place, bottom near this elongated tubular accommodation space.
Kapillary for thermal convection polymerase chain reaction device of the present invention, wherein, this high heat conduction ring is metal ring.
Kapillary for thermal convection polymerase chain reaction device of the present invention, wherein, this high heat conduction ring is that copper becomes.
Kapillary for thermal convection polymerase chain reaction device of the present invention, wherein, this body has a pipe lid.
Kapillary for thermal convection polymerase chain reaction device of the present invention, wherein, the tapered long and narrow tubular space that becomes in the bottom of this elongated tubular accommodation space.
Accordingly, when this elongated tubular accommodation space filling is reacted sample and is added the thermogenesis thermal convection, because the distance of the left and right edges of this axial cross section is greater than the distance at the edge, front and back of this axial cross section, therefore the left and right edges of this axial cross section can form narrower passage, compared to known, the invention has the advantages that the flow velocity that can reduce the thermal convection rising and prolong the cycling time of thermal convection, that is prolong the reaction times of reacting sample, therefore can increase the reaction efficiency of thermal convection polymerase chain reaction device.
Description of drawings
Fig. 1 is known capillary pipe structure figure;
Fig. 2 is known kapillary placing structure figure;
Fig. 3 is capillary pipe structure figure of the present invention;
Fig. 4 is kapillary placing structure figure of the present invention;
Fig. 5 is kapillary placing structure part sectioned view of the present invention;
Fig. 6 is that kapillary of the present invention uses schematic diagram.
Embodiment
Below will cooperate accompanying drawing shown in the specific embodiment that the present invention is described in detail.
See also Fig. 3, Fig. 4 and shown in Figure 5, the invention provides a kind of kapillary for thermal convection polymerase chain reaction device, for being arranged on the test tube flat 20, it comprises a body 11, this body 11 has an elongated tubular accommodation space 12, this elongated tubular accommodation space 12 tools one axial cross section 13, the distance of the left and right edges 131 of this axial cross section 13 is greater than the distance at the edge, front and back 132 of this axial cross section 13, this axial cross section 13 can be occluding surface, the curvature of axial cross section 13 left and right edges 131 is greater than the curvature at this edge, axial cross section 13 front and back 132, can be elliptical area (it is that example is illustrated that this axial cross section 13 is drawn elliptical area) such as this axial cross section 13, perhaps axial cross section 13 also can be rectangle.
This body 11 can enclose around a high heat conduction ring 30 in the place, bottom near this elongated tubular accommodation space 12, and this high heat conduction ring 30 can be metal ring, as becoming for copper.And this body 11 can have a pipe and cover 40, and the bottom of this elongated tubular accommodation space 12 can taperedly become a long and narrow tubular space 121, to increase identity.
See also Fig. 6, when these elongated tubular accommodation space 12 fillings are reacted samples 50 and are added thermogenesis thermal convection 51, because the distance of the left and right edges 131 of this axial cross section 13 is greater than the distance at the edge, front and back 132 of this axial cross section 13, and different curvature can be had in the left and right edges 131 of this axial cross section 13 and edge, front and back 132, therefore the left and right edges 131 of this axial cross section 13 forms narrower passage, it can reduce the flow velocity that thermal convection 51 is risen, and prolong the cycling time of thermal convection 51, but namely prolong the reaction times of reaction sample 50, therefore can increase the reaction efficiency of thermal convection polymerase chain reaction device.
As mentioned above, the present invention discloses a kind of kapillary of non-circle, can reduce the flow velocity of thermal convection 51, but and the reaction times of prolongation reaction sample 50 therefore can increase the reaction efficiency of thermal convection polymerase chain reaction device, is satisfied the demand on using.
Claims (9)
1. a kapillary that is used for thermal convection polymerase chain reaction device for being arranged on the test tube flat (20), is characterized in that, comprises:
One body (11), this body (11) has an elongated tubular accommodation space (12), this elongated tubular accommodation space (12) tool one axial cross section (13), the distance of the left and right edges (131) of this axial cross section (13) is greater than the distance at the edge, front and back (132) of this axial cross section (13).
2. the kapillary for thermal convection polymerase chain reaction device according to claim 1, it is characterized in that, this axial cross section (13) is occluding surface, and the curvature of this axial cross section (13) left and right edges (131) is greater than the curvature of edge (132) before and after this axial cross section (13).
3. the kapillary for thermal convection polymerase chain reaction device according to claim 2 is characterized in that, this axial cross section (13) is elliptical area.
4. the kapillary for thermal convection polymerase chain reaction device according to claim 1 is characterized in that, this axial cross section (13) is rectangle.
5. the kapillary for thermal convection polymerase chain reaction device according to claim 1 is characterized in that, this body (11) encloses around a high heat conduction ring (30) in the place, bottom near this elongated tubular accommodation space (12).
6. the kapillary for thermal convection polymerase chain reaction device according to claim 5 is characterized in that, this high heat conduction ring (30) is metal ring.
7. the kapillary for thermal convection polymerase chain reaction device according to claim 6 is characterized in that, this high heat conduction ring (30) becomes for copper.
8. the kapillary for thermal convection polymerase chain reaction device according to claim 1 is characterized in that, this body (11) has a pipe lid (40).
9. the kapillary for thermal convection polymerase chain reaction device according to claim 1 is characterized in that, the tapered long and narrow tubular space (121) that becomes in the bottom of this elongated tubular accommodation space (12).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101984771A CN102876569A (en) | 2011-07-11 | 2011-07-11 | Capillary tube for heat convection polymerase chain reaction device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101984771A CN102876569A (en) | 2011-07-11 | 2011-07-11 | Capillary tube for heat convection polymerase chain reaction device |
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| CN102876569A true CN102876569A (en) | 2013-01-16 |
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| CN2011101984771A Pending CN102876569A (en) | 2011-07-11 | 2011-07-11 | Capillary tube for heat convection polymerase chain reaction device |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434320A (en) * | 2016-11-15 | 2017-02-22 | 上海快灵生物科技有限公司 | Micro-solution reactor |
| CN109207645A (en) * | 2018-11-01 | 2019-01-15 | 福建省博凯科技有限公司 | The control method of one-part form thermal convection instrument |
| CN109554295A (en) * | 2019-01-21 | 2019-04-02 | 武汉理工大学 | The PCR amplification and disease detection device of Oceanic tracking ship |
| CN112899151A (en) * | 2021-02-01 | 2021-06-04 | 青岛迪诺瓦基因科技有限公司 | Flowing liquid temperature changing device and using method thereof |
| WO2021254512A1 (en) * | 2020-06-19 | 2021-12-23 | 上海快灵生物科技有限公司 | Tube-breaking structure, biochemical test tube and heat-conducting blind tube |
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| CN1571849A (en) * | 2001-09-15 | 2005-01-26 | 阿赫姆生物系统公司 | Method and apparatus for amplification of nucleic acid sequences by using thermal convection |
| CN101004287A (en) * | 2006-01-19 | 2007-07-25 | 博奥生物有限公司 | Heating up parts of capillary |
| CN101522909A (en) * | 2006-05-17 | 2009-09-02 | 加利福尼亚技术学院 | Thermal cycling system |
| EP2140026A2 (en) * | 2006-11-03 | 2010-01-06 | Siemens Healthcare Diagnostics Inc. | Random access system and method for polymerase chain reaction testing |
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| CN201753992U (en) * | 2010-07-26 | 2011-03-02 | 山东太阳雨太阳能有限公司 | Full glass double-vacuum heat pipe protection device |
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2011
- 2011-07-11 CN CN2011101984771A patent/CN102876569A/en active Pending
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| CN1571849A (en) * | 2001-09-15 | 2005-01-26 | 阿赫姆生物系统公司 | Method and apparatus for amplification of nucleic acid sequences by using thermal convection |
| CN101004287A (en) * | 2006-01-19 | 2007-07-25 | 博奥生物有限公司 | Heating up parts of capillary |
| CN101522909A (en) * | 2006-05-17 | 2009-09-02 | 加利福尼亚技术学院 | Thermal cycling system |
| EP2140026A2 (en) * | 2006-11-03 | 2010-01-06 | Siemens Healthcare Diagnostics Inc. | Random access system and method for polymerase chain reaction testing |
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| CN102803465A (en) * | 2010-01-12 | 2012-11-28 | 阿赫姆生物系统公司 | Two-stage thermal convection apparatus and uses thereof |
| CN201753992U (en) * | 2010-07-26 | 2011-03-02 | 山东太阳雨太阳能有限公司 | Full glass double-vacuum heat pipe protection device |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434320A (en) * | 2016-11-15 | 2017-02-22 | 上海快灵生物科技有限公司 | Micro-solution reactor |
| CN109207645A (en) * | 2018-11-01 | 2019-01-15 | 福建省博凯科技有限公司 | The control method of one-part form thermal convection instrument |
| CN109554295A (en) * | 2019-01-21 | 2019-04-02 | 武汉理工大学 | The PCR amplification and disease detection device of Oceanic tracking ship |
| WO2021254512A1 (en) * | 2020-06-19 | 2021-12-23 | 上海快灵生物科技有限公司 | Tube-breaking structure, biochemical test tube and heat-conducting blind tube |
| CN112899151A (en) * | 2021-02-01 | 2021-06-04 | 青岛迪诺瓦基因科技有限公司 | Flowing liquid temperature changing device and using method thereof |
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Inventor after: Su Cheng Inventor after: Deng Binghua Inventor after: Zheng Jianzong Inventor before: Su Cheng Inventor before: Deng Binghua |
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Free format text: CORRECT: INVENTOR; FROM: SU CHENG DENG BINGHUA TO: SU CHENG DENG BINGHUA ZHENG JIANZONG |
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Application publication date: 20130116 |