CN209652319U - A kind of vent cabinet and pig circular ring virus PCR sensing chamber - Google Patents
A kind of vent cabinet and pig circular ring virus PCR sensing chamber Download PDFInfo
- Publication number
- CN209652319U CN209652319U CN201821481533.6U CN201821481533U CN209652319U CN 209652319 U CN209652319 U CN 209652319U CN 201821481533 U CN201821481533 U CN 201821481533U CN 209652319 U CN209652319 U CN 209652319U
- Authority
- CN
- China
- Prior art keywords
- buffering
- area
- blower
- preparation
- corridor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model discloses a kind of vent cabinet and pig circular ring virus PCR sensing chamber, including closed reagent area in preparation, between first buffering, sample preparation zone, between second buffering, nucleic acid amplification area, between third buffering, amplified production analysis area, between 4th buffering, corridor and supply air system, the corridor is standard pressure, on the basis of standard pressure, wherein between the first buffering, between second buffering, between third buffering, between 4th buffering, the air pressure of reagent area in preparation is zone of positive pressure, the utility model has the beneficial effects that passing through supply air system, guarantee that there is certain pressure difference between each compartment, to control the direction of air-flow flowing, action for each compartment is different, pollution source apportionment is different, to control the flow direction of pollution sources, both the accuracy of testing result had been ensure that, prevent the pollutant generated in detection process from spreading again, Pollute ambient enviroment.
Description
Technical field
The utility model belongs to animal detection technique field, and in particular to a kind of vent cabinet and pig circular ring virus PCR detection
Room.
Background technique
When carrying out pig circular ring virus PCR detection, preparation, preparation, amplification and the multiple steps of analysis of reagent are needed
It carries out.In order to guarantee that the accuracy of detection, each step need to carry out in different sections, to prevent reagent contaminated and prevent
The diffusion pollution others section of reagent, according to the difference of the action of each step, the control mode of pollution sources is different.
Utility model content
Guarantee that the accuracy of detection, each step need to carry out in different sections in the prior art, to prevent
Only reagent is contaminated and prevents the diffusion pollution others section of reagent, according to the difference of the action of each step, pollution
The control mode different problems in source provide a kind of vent cabinet and pig circular ring virus PCR sensing chamber.
A kind of vent cabinet, including cabinet body, perpendicular baffle, wind deflector, travel switch, the first blower and the second blower, the cabinet
Body top is hood, and the hood top is exhaust pipe, and the perpendicular baffle is fixedly installed on vertically in exhaust pipe will exhaust
Pipe is divided into two gas circuits in left and right, and the wind deflector made of lightweight material connect with perpendicular flapper, and first blower passes through the
One pipeline is connected to the left side road of exhaust pipe, and second blower is connected to by the second pipeline with the right side road of exhaust pipe, described
First blower and the second fan parallel-connection, wherein travel switch and indicator light are in series in the parallel branch of the second blower, the row
Cheng Kaiguan is set on interior of the cabinet hood, positioned at the lower section of left side wind deflector.
Between a kind of vent cabinet and pig circular ring virus PCR sensing chamber, including closed reagent area in preparation, the first buffering, sample
Prepare between area, the second buffering, nucleic acid amplification area, between third buffering, between amplified production analysis area, the 4th buffering, corridor and air-supply
System,
The reagent area in preparation is adjacent with sample preparation zone, and is connected to by the first pass-through box,
The sample preparation zone is adjacent with nucleic acid amplification area, and is connected to by the second pass-through box,
The accounting amplification region is adjacent with amplified production analysis area, and is connected to by third pass-through box,
It is set between reagent area in preparation and corridor between first buffering,
It is set between sample preparation zone and corridor between second buffering,
It is set between nucleic acid amplification area and corridor between the third buffering,
It is set between amplified matter analysis area and corridor between 4th buffering,
The corridor is standard pressure, on the basis of standard pressure, wherein between the first buffering, between the second buffering, third it is slow
Between punching, between the 4th buffering, the air pressure of reagent area in preparation be zone of positive pressure, the air pressure of the reagent area in preparation is higher than between the first buffering
Air pressure, the sample preparation zone, calculate amplification region, the air pressure of amplified production analysis area be negative pressuren zone, and air pressure is successively
It reduces,
The supply air system be first buffering between, second buffering between, third buffering between, the 4th buffering between, reagent area in preparation,
Amplification region, the air-supply exhaust of amplified production analysis area are calculated in sample preparation zone, keep the pressure difference between them,
The sample preparation zone calculates amplification region, is provided with vent cabinet in amplified production analysis area.
Preferably, pig circular ring virus PCR sensing chamber, the corridor is standard pressure, on the basis of standard pressure,
In first buffering between, second buffering between, third buffering between, the 4th buffering between 42 air pressure be+10Pa, the reagent area in preparation 11
Air pressure be+15Pa, 21 air pressure of sample preparation zone be -10Pa, it is described calculate amplification region 31 air pressure be -15Pa, it is described
The air pressure of amplified production analysis area 41 is -20Pa.
Preferably, by the way of the supply air system send side to arrange using top.
The utility model has the beneficial effects that
1. there are two blowers for vent cabinet, in the case where a failure, another can start automatically, improve the steady of equipment
It is qualitative, it prevents in laboratory blower catastrophic failure, pollutant is spread to damage to operator.
2. guaranteeing that there is certain pressure difference between each compartment by supply air system, to control the direction of air-flow flowing, needle
Different to the action of each compartment, pollution source apportionment is different, to control the flow direction of pollution sources, both ensure that detection knot
The accuracy of fruit, and prevent the pollutant generated in detection process from spreading, pollute ambient enviroment.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of an embodiment of the present invention vent cabinet；
Fig. 2 is that an embodiment of the present invention fume hood fan connects electrical schematic diagram；
Fig. 3 is the structural schematic diagram of an embodiment of the present invention vent cabinet；
Fig. 4 is the distribution map of an embodiment of the present invention control laboratory.
Specific embodiment
Below with reference to the attached drawing in the utility model embodiment, the technical solution of the utility model is described further.
A kind of vent cabinet, including cabinet body 1, perpendicular baffle 3, wind deflector 4, travel switch 6, the first blower 7 and the second blower 8,
1 top of cabinet body is hood 15, and 15 top of hood is exhaust pipe 16, and the perpendicular baffle 3 is solid vertically
Surely it is set in exhaust pipe 16 and exhaust pipe 16 is divided into two gas circuits in left and right, the wind deflector 4 made of lightweight material and perpendicular baffle 3
Rotation connection,
First blower 7 is connected to by the first pipeline 71 with the left side road of exhaust pipe 16, and second blower passes through the
Two pipelines 82 are connected to the right side road of exhaust pipe 16,
First blower 7 and the second blower 8 are in parallel, wherein being in series with travel switch 6 in the parallel branch of the second blower 8
With indicator light 81,
The travel switch 6 is set on 1 exhaust gas inside cover 15 of cabinet body, positioned at the lower section of left side wind deflector 4.
As shown in Figure 1, can be operated inside cabinet body 1, the venomous injurant of generation when the operation tested
Matter is discharged by exhaust system, prevents from impacting health of human body.The first blower 7 is common blower in figure, as shown in Fig. 2,
It is closed master switch 9, the first blower 7 starts exhausting work at this time, at this point, under the action of the wind, as shown in Figure 1, a left side for perpendicular baffle 3
The wind deflector 4 of side rotates clockwise, and the gas inside cabinet body 1 routes the extraction of the first blower 7 by the left side of exhaust pipe 16.When
When one blower 7 breaks down, the rotation counterclockwise under the effect of gravity of wind deflector 4 drops back into horizontality, as shown in figure 3, touching
Travel switch 6 is sent out, normally opened travel switch 6 is closed, as shown in Fig. 2, the second blower 8 is switched on, indicator light 81 is bright, is made in wind-force
Under, right side wind deflector 4 is rotated counterclockwise, and the gas inside cabinet body 1 is discharged from the right side of exhaust pipe 16.Guarantee to lead to as a result,
The stability of the work of wind cabinet, even if the first blower 7 breaks down, the second blower of self-starting 8 is exhausted, and prevents pollution sources from expanding
It dissipates, damages operator's health.Meanwhile indicator light 81 is fan trouble indicator light, for reminding user's on-call maintenance.
12, sample preparation between a kind of pig circular ring virus PCR sensing chamber, including the buffering of closed reagent area in preparation 11, first
42 between 32, amplified production analysis area the 41, the 4th buffers between 22, nucleic acid amplification area 31, third buffering between the buffering of area 21, second, walk
Corridor 5, supply air system and above-mentioned vent cabinet,
The reagent area in preparation 11 is adjacent with sample preparation zone 21, and is connected to by the first pass-through box 61,
The sample preparation zone 21 is adjacent with nucleic acid amplification area 31, and is connected to by the second pass-through box 62,
The accounting amplification region 31 is adjacent with amplified production analysis area 41, and is connected to by third pass-through box 63,
12 are set between reagent area in preparation 11 and corridor 5 between first buffering,
22 are set between sample preparation zone 21 and corridor 5 between second buffering,
32 are set between nucleic acid amplification area 31 and corridor 5 between the third buffering,
42 are set between amplified matter analysis area 41 and corridor 5 between 4th buffering,
The corridor 5 is standard pressure, on the basis of standard pressure, wherein between the first buffering 22, the between the 12, second buffering
42 air pressure is+10Pa between the 32, the 4th buffering between three bufferings, and the air pressure of the reagent area in preparation 11 is+15Pa, the sample system
21 air pressure of preparation area is -10Pa, and the air pressure for calculating amplification region 31 is -15Pa, the air pressure of the amplified production analysis area 41 is -
20Pa,
The supply air system be first buffering between 12, second buffering between 22, third buffering between the 32, the 4th buffering between 42, examination
Amplification region 31, the air-supply exhaust of amplified production analysis area 41 are calculated in agent area in preparation 11, sample preparation zone 21, keep between them
Pressure difference,
The sample preparation zone 21 calculates amplification region 31, is provided with vent cabinet in amplified production analysis area 41.
Preferably, by the way of supply air system send side to arrange using top.
Door is provided between buffering between each workspace, door is opened to the side towards high pressure, can be with by high air pressure
Door is quickly closed, leakage is reduced.
As shown in figure 4,
Reagent area in preparation 11: for zone of positive pressure ,+15Pa
12 between first buffering: for zone of positive pressure ,+10Pa, staff is initially entered 12 between the first buffering, herein replacement experiment
The preparations such as clothes, then enter back into reagent area in preparation 11.
Major function: the storage of original-pack reagent (commercial kit), the preparation and packing of reaction mixture.
Work: for example, n parts of samples, prepare n+1 parts of PCR reaction solutions: (n+1) × 16 μ LPCR buffer adds (n+1)
× 2 μ LTaq archaeal dna polymerases, take 18 μ L to be distributed into n parts, in PCR reaction tube, cover tightly lid after mixing.
Airflow direction: 12 → corridor 5 between reagent area in preparation 11 → the first buffers.Guarantee that one's respective area cleaning is pollution-free, prevents
Reagent is by the nucleic acid-templated pollution of PCR.
Air-supply: top send side to arrange, and guarantees that Laboratory air is clean.
Since the work carried out in reagent area in preparation 11 does not generate pollution sources, meanwhile, sample has contaminated danger, institute
With air pressure is successively reduced by reagent area in preparation 11, first buffering area 12 to corridor 5, prevents the pollution outside reagent area in preparation 11
Source enters, and the air-flow outflow inside reagent area in preparation 11 will not pollute.
Sample preparation zone 21: for negative pressuren zone, -10Pa.
22 between second buffering: for zone of positive pressure ,+10Pa.Staff is introduced into 22 between the second buffering, replaces lab-gown herein
Etc. preparations, subsequently into sample preparation zone 21.
The working contents of sample preparation zone 21: the preservation of sample；Nucleic acid extraction, preservation；It is positive, negative controls
It saves；The mixing of PCR amplification system and template.
Work: for example, n parts of pig blood samples and n parts of PCR reaction solutions are passed to by the first pass-through box 61 from reagent area in preparation 11
Sample preparation zone 21.Blood sample is handled according to the requirement of kit specification, extracts the DNA of pig circular ring virus, it is final to obtain
The template of acquisition is added to PCR reaction solution by template DNA.
Airflow direction are as follows: 22 → sample preparation zone 21 between corridor 5 ← the second buffers.The gas for preventing sample preparation zone 21 from being formed
Colloidal sol is spread to close region, pollutes adjacent area.The pollutant in corridor 5 cannot enter the region.
Air-supply: top send side to arrange, and guarantees that Laboratory air is clean.
Pollution sources: blood sample virus can be spread with aerosol or formation aerosol；The gas that sample DNA is formed is molten
Glue.
Nucleic acid amplification area 31: for negative pressuren zone, -15Pa.
32 between third buffering: for zone of positive pressure ,+10Pa.Staff is introduced into 32 between third buffering, replaces lab-gown herein
Etc. preparations, subsequently into nucleic acid amplification area 31.
Major function: nucleic acid amplification.
Work: PCR reaction tube is passed to by the second pass-through box 62, is expanded in PCR amplification instrument.
Airflow direction are as follows: between corridor ← third buffering → nucleic acid amplification area, avoid aerosol from leaking out from local area, the dirt in corridor
Dye object cannot enter the region.
Air-supply: side is sent to arrange in top.Guarantee that Laboratory air is clean.
Pollution sources: the aerosol of (a large amount of) formation of amplified production DNA.
Amplified production analysis area 41: for negative pressuren zone, -20Pa
42 between 4th buffering: for zone of positive pressure ,+10Pa.Staff is introduced into 42 between the 4th buffering, replaces lab-gown herein
Etc. preparations, subsequently into amplified production analysis area 41.
Major function: nucleic acid product is detected and confirmed.
Work: after amplification, PCR reaction tube is passed to by third pass-through box 63.Nucleic acid electrophoresis: opening reaction tube, to
Point sample in glue hole, it is rear to cross gel imaging system after electrophoresis, obtain electrophoresis picture.
Airflow direction are as follows: between corridor ← the 4th buffering → amplified production analysis area.Prevent DNA diffusion pollution.The dirt in corridor
Dye object will not enter the region.
Air-supply: side is sent to arrange in top.Guarantee that Laboratory air is clean.
Pollution sources: the aerosol that amplified production is formed.
Because of aerosol meeting diffusion pollution, the false positive of detection is caused.Reasonable subregion and pressure design can be molten to avoid gas
The diffusion of glue.
The operation for having pollution sources diffusion dangerous is operated in vent cabinet, ensure the health of operator not by
Infringement.
It will be appreciated that the embodiments of the present invention while there has been shown and described that, for the common skill of this field
For art personnel, it is possible to understand that can be carried out to these embodiments in the case where not departing from the principles of the present invention and spirit
A variety of change, modification, replacement and modification, the scope of the utility model are defined by the appended claims and the equivalents thereof.
Claims (1)
1. a kind of vent cabinet, which is characterized in that including cabinet body (1), perpendicular baffle (3), wind deflector (4), travel switch (6), first
Blower (7) and the second blower (8),
Cabinet body (1) top is hood (15), and hood (15) top is exhaust pipe (16), the perpendicular baffle (3)
It is fixedly installed in exhaust pipe (16) vertically and exhaust pipe (16) is divided into two gas circuits in left and right, the wind deflector made of lightweight material
(4) it is rotatablely connected with perpendicular baffle (3),
First blower (7) is connected to by the first pipeline (71) with the left side road of exhaust pipe (16), and second blower passes through
Second pipeline (82) is connected to the right side road of exhaust pipe (16),
First blower (7) and the second blower (8) are in parallel, open wherein being in series with stroke in the parallel branch of the second blower (8)
(6) and indicator light (81) are closed,
The travel switch (6) is set on cabinet body (1) exhaust gas inside cover (15), is located at the lower section of left side wind deflector (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821481533.6U CN209652319U (en) | 2018-09-11 | 2018-09-11 | A kind of vent cabinet and pig circular ring virus PCR sensing chamber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821481533.6U CN209652319U (en) | 2018-09-11 | 2018-09-11 | A kind of vent cabinet and pig circular ring virus PCR sensing chamber |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209652319U true CN209652319U (en) | 2019-11-19 |
Family
ID=68505371
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821481533.6U Active CN209652319U (en) | 2018-09-11 | 2018-09-11 | A kind of vent cabinet and pig circular ring virus PCR sensing chamber |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209652319U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113388507A (en) * | 2021-06-09 | 2021-09-14 | 北京卓诚惠生生物科技股份有限公司 | Nucleic acid extraction and PCR detection all-in-one machine |
-
2018
- 2018-09-11 CN CN201821481533.6U patent/CN209652319U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113388507A (en) * | 2021-06-09 | 2021-09-14 | 北京卓诚惠生生物科技股份有限公司 | Nucleic acid extraction and PCR detection all-in-one machine |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN204278043U (en) | Aseptic processing isolator | |
| JP2009533038A (en) | Mobile laboratory for pathogen analysis | |
| NZ742682A (en) | Process of operating the air conditioning system of a cab of a machine | |
| CN209652319U (en) | A kind of vent cabinet and pig circular ring virus PCR sensing chamber | |
| KR20180023665A (en) | System for providing air quality information | |
| CN104275217B (en) | A kind of Multifunctional combined experimental case for haze environment simulation | |
| CN206508993U (en) | A kind of multifunctional testing cabin | |
| CN212453801U (en) | Integrated mobile pathogen nucleic acid detection laboratory system | |
| CN207133092U (en) | Natural haze gas collection concentration experimental provision | |
| CN206653889U (en) | A kind of automobile-used purifier | |
| CN105973784A (en) | Solid particle protective clothing detection experiment chamber | |
| CN108624491A (en) | A kind of packaged type PCR Lab | |
| CN205982220U (en) | Smell, VOC and showy harmful substance checkout room with illumination testing capabilities | |
| CN103818295A (en) | Biological safety inspection vehicle | |
| Clark | Safety cabinets, fume cupboards and other containment systems | |
| CN203727245U (en) | Bio-safety detecting vehicle | |
| Alonso et al. | An evaluation of interventions for reducing the risk of PRRSV introduction to filtered farms via retrograde air movement through idle fans | |
| CN206661875U (en) | A kind of ventilation hoods for laboratories | |
| JP6652902B2 (en) | Clean room | |
| CN212538175U (en) | Anti-airflow and anti-serial dual-purpose ventilation PCR laboratory | |
| CN205748830U (en) | A kind of dry heat sterilizer high efficiency particulate air filter leak detection system and dry heat sterilizer | |
| CN208512601U (en) | The embedded waste liquid of experimental bench customizes cabinet | |
| CN207614085U (en) | It is equipped with the ventilation duct of automation sterilizing equipment decontaminating apparatus | |
| CN106290746B (en) | A kind of peculiar smell test box | |
| CN207096219U (en) | Environmental chamber, which is smelt, distinguishes analytical equipment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: 266000 room 605, Jufeng venture building, No.52, Miaoling Road, Laoshan District, Qingdao City, Shandong Province Patentee after: Qingdao Haihua Biological Group Co.,Ltd. Address before: No. 458, Jiangshan South Road, Huangdao District, Qingdao, Shandong 266000 Patentee before: QINGDAO HAIHUA BIOMEDICAL TECHNOLOGY Co.,Ltd. |