CN113384507A - Anti-aging composition and preparation method and application thereof - Google Patents
Anti-aging composition and preparation method and application thereof Download PDFInfo
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- CN113384507A CN113384507A CN202110831489.7A CN202110831489A CN113384507A CN 113384507 A CN113384507 A CN 113384507A CN 202110831489 A CN202110831489 A CN 202110831489A CN 113384507 A CN113384507 A CN 113384507A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/494—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom
- A61K8/4946—Imidazoles or their condensed derivatives, e.g. benzimidazoles
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/494—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom
- A61K8/4953—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom containing pyrimidine ring derivatives, e.g. minoxidil
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/673—Vitamin B group
- A61K8/675—Vitamin B3 or vitamin B3 active, e.g. nicotinamide, nicotinic acid, nicotinyl aldehyde
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
Abstract
The invention provides an anti-aging composition, a preparation method and an application thereof, wherein the anti-aging composition comprises the following components: tea leaf extract, watercress extract, horsetail extract, nettle leaf extract, silybum marianum fruit extract, tetrahydro-methyl pyrimidine carboxylic acid and ergothioneine. The invention provides the mutual cooperation of the effective components, under the premise of not influencing the respective functions, the synergistic effect ensures that the obtained anti-aging composition can prevent skin aging from various aspects such as free radical removal, oxidation resistance, saccharification resistance, pollution resistance and the like, has excellent effect, and keeps the health and youth of the skin.
Description
Technical Field
The invention belongs to the technical field of cosmetic preparation, and relates to an anti-aging composition, and a preparation method and application thereof.
Background
The skin is the first line of defense of the human body against external pressure. However, as the pollution increases, the particles in the air pollutants adhere to the skin surface, easily causing dull color and luster to the skin, and causing skin aging. Besides environmental pollution, skin aging is easily caused by problems such as light, sugar intake, and oxidation. Skin aging is an irreversible process, and although the existing facial anti-aging wrinkle removal methods and products are various, the skin aging is caused by more reasons, and the anti-aging method of the cosmetics in the prior art is single. Therefore, delaying skin aging, reducing facial wrinkles and maintaining skin vitality are still a big topic of research in the field of cosmetics at present.
CN106109362A provides an anti-aging composition, an anti-aging cosmetic and a preparation method thereof. In the anti-aging composition, the plantain seed extract, the lupin extract and the lotus extract are compounded for use, so that the anti-aging composition can effectively prevent and improve skin aging, can tighten skin and has the effect of resisting skin aging. Meanwhile, the anti-aging composition is used as an active component, and other raw materials are supplemented to prepare anti-aging skin lotion, anti-aging emulsion and anti-aging cream, so that the anti-aging skin cream can effectively play a role in resisting skin aging and can meet the use requirements of different people and different occasions.
CN108703909A provides a cosmetic with whitening, moisturizing and anti-wrinkle effects and a preparation method thereof, wherein the cosmetic is prepared from the following raw materials in parts by weight: 130-150 parts of deionized water, 0.6-0.8 part of xanthan gum, 6-8 parts of sodium cocoyl sarcosinate, 1-1.5 parts of erythritol, 1-1.5 parts of acetyl chitosamine, 4-5 parts of sodium lauryl amphoacetate, 0.8-1 part of ascorbyl glucoside, 2.5-3 parts of arbutin, 8-10 parts of butanediol, 4-5 parts of glycerol, 2.5-3 parts of glycyrrhiza glabra extract, 2-4 parts of bacillus coagulans juglandis pericarpium juglandis, 2-4 parts of pleurotus persicae extract, 3-5 parts of green tea extract, 2.5-3 parts of chamomile extract and 1-1.5 parts of glyceryl undecylenate. The cosmetic has excellent whitening and moisturizing effects; and outstanding anti-wrinkle and anti-aging effects; high stability, less irritation, and good effect of relieving and resisting allergy.
However, it is difficult to achieve anti-aging effects in many aspects including the above cosmetics, and aging is often inhibited only by one factor, but not by inhibition of saccharification, oxidation, free radicals, pollution, and the like.
Therefore, it is necessary to provide an anti-aging composition having various effects of eliminating active oxygen, preventing pollution, resisting photoaging, resisting saccharification and the like, so as to meet the market demand.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide an anti-aging composition, a preparation method and application thereof, wherein the anti-aging composition can prevent skin aging from the aspects of free radical removal, oxidation resistance, saccharification resistance, pollution resistance and the like, reduce wrinkle generation and ensure the young and healthy skin.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the present invention provides an anti-aging composition comprising: tea (CAMELLIA SINENSIS) leaf extract, watercress (NASTURTIUM OFFICINALE) extract, field horsetail (EQUISETUM ARVENSE) extract, Urtica DIOICA (URTICA) leaf extract, Silybum MARIANUM (Silybum MARIANUM) fruit extract, tetrahydropyrimidine carboxylic acid and ergothioneine.
In the invention, the tea (CAMELLIA SINENSIS) leaf extract contains tea polyphenol components, has strong oxidation resistance, can efficiently eliminate active oxygen, and simultaneously clears or lightens secondary pigment precipitation.
In the invention, the watercress (NASTURTIUM OFFICINALE) extract is preferably a watercress (NASTURTIUM OFFICINALE) flower/leaf/stem extract, has obvious effect of inhibiting sebum secretion, and has prevention and treatment effects on skin diseases such as acne caused by excessive sebum secretion and easy development; herba Equiseti Arvinsis (Equisetum ARVENSE) extract can be used as skin-activating and antiaging agent; the extract of leaves of Urtica DIOICA (Urtica DIOICA) can improve the function of skin immunocyte, and can be used as antiinflammatory agent, free radical scavenging agent, and antioxidant.
In the invention, the SILYBUM MARIANUM (SILYBUM MARIANUM) fruit extract can capture oxygen free radicals, has strong oxidation resistance, combines good inhibition on elastase and astringency on skin pores, and can be used in anti-aging cosmetics for removing wrinkles.
In the invention, the tetrahydro-methyl pyrimidine carboxylic acid is also called Ectoin (Ectoin), is an amino acid derivative, plays a chemical transmitter-like role in cells, has a strong anti-compression protection function, can bind water molecules around to form a complex, and wraps around cells, enzymes, proteins and other organic molecules to form a protective shell with the functions of protecting, nourishing and stabilizing hydration, thereby resisting the radiation damage of infrared rays to the skin. As the tetrahydromethylpyrimidine carboxylic acid in the present invention, commercially available products can be used, and preferably, the tetrahydromethylpyrimidine carboxylic acid produced by Bitop corporation is used.
In the invention, L-Ergothioneine (EGT) is a rare natural chiral amino acid, is an important natural active substance in an organism, has strong antioxidant activity, protects mitochondria from being damaged by oxidation, protects the activity of AP-1, and inhibits elastase.
Therefore, in the invention, the tea (CAMELLIA SINENSIS) leaf extract, the watercress (NASTURTIUM OFFICINALE) extract, the field horsetail (EQUISETUM ARVENSE) extract, the URTICA DIOICA (URTICA) leaf extract, the SILYBUM MARIANUM (SILYBUM MARIANUM) fruit extract, the tetrahydromethylpyrimidine carboxylic acid and the ergothioneine are mixed, all the substances are matched with each other, under the condition of not influencing respective functions, the synergistic effect is realized, the antioxidant, anti-saccharification, anti-pollution and other effects of the obtained anti-aging composition are obviously improved, and the anti-aging composition can be added into a cosmetic matrix to obtain a cosmetic with good anti-aging effect.
In a preferred embodiment of the present invention, the mass ratio of the watercress extract, the equisetum arvense extract and the nettle leaf extract is 1 (0.5-1.5): (1.5-3), and may be, for example, 1:0.5:1.5, 1:0.5:2, 1:0.5:2.5, 1:0.5:3, 1:1:1.5, 1:1.5:1.5, 1:1:2, 1:0.8:2.5 or 1:1.5: 3.
Preferably, the mass ratio of the tea extract, the tetrahydro-methylpyrimidine carboxylic acid and the ergothioneine is 1 (5-10): (0.8-2), and may be, for example, 1:5:0.8, 1:5:1, 1:5:1.5, 1:5:2, 1:6:0.8, 1:8:0.8, 1:10:0.8, 1:8:1 or 1:10: 2.
As a preferred technical scheme, the anti-aging composition further comprises a skin conditioner.
Preferably, the skin conditioning agent comprises any one of vitamin E, carnosine, niacinamide, or sodium hyaluronate, or a combination of at least two of the same. For example, vitamin E, tocopherol, is one of the most used anti-aging ingredients, belongs to the main fat-soluble antioxidants of human body, and can scavenge free radicals in vivo to block the generation of oxidation reaction, and relieve and repair the damage of cell membranes, thereby achieving the effect of resisting aging.
Preferably, the anti-aging composition further comprises a humectant.
Preferably, the humectant comprises any one of glycerol, ethanol, butylene glycol, or a combination of at least two thereof.
As a preferred embodiment of the present invention, the anti-aging composition further comprises an emulsifier.
Preferably, the anti-aging composition further comprises a penetrant.
Preferably, the anti-aging composition further comprises a thickener.
Preferably, the anti-aging composition further comprises a preservative.
As a preferable technical scheme of the invention, the anti-aging composition comprises the following components in percentage by mass:
1-10% of tea extract, 1-5% of watercress extract, 1-5% of field horsetail extract, 1-5% of nettle leaf extract, 3-10% of silybum marianum fruit extract, 1-10% of tetrahydro-methylpyrimidine carboxylic acid, 1-5% of ergothioneine, 0.1-5% of vitamin E, 0.1-3% of carnosine, 0.5-1% of hydrogenated lecithin, 0.5-1% of lecithin, 1-3% of thickening agent, 5-30% of humectant, 0.1-2% of penetrating agent, 1-3% of pH regulator, 0.5-1% of preservative and the balance of water.
In the invention, the content of the tea extract can be 2%, 3%, 4%, 5%, 6%, 7%, 8% or 9% by mass; the mass percentage content of the watercress extract can be 2%, 3% or 4% and the like; the mass percentage content of the horsetail extract can be 2%, 3% or 4% and the like; the mass percentage content of the nettle leaf extract can be 2%, 3% or 4% and the like; the mass percentage of the silybum marianum fruit extract can be 4%, 5%, 6%, 7%, 8% or 9% and the like; the weight percentage of the tetrahydromethyl pyrimidine carboxylic acid can be 2%, 3%, 4%, 5%, 6%, 7%, 8% or 9% and the like; the ergothioneine can be 2%, 3% or 4% in percentage by mass; the mass percentage of the vitamin E can be 0.5%, 1%, 2%, 3% or 4% and the like; the mass percentage of the carnosine can be 0.2%, 0.5%, 1%, 2% or 2.5% and the like; the mass percentage of the hydrogenated lecithin can be 0.6 percent, 0.8 percent or 0.9 percent, etc.; the mass percentage of the lecithin can be 0.6%, 0.8% or 0.9% and the like; the mass percentage of the thickening agent can be 1.5%, 2% or 2.5% and the like; the mass percentage of the humectant can be 6%, 8%, 10%, 12%, 15%, 18%, 20%, 22%, 25% or 28% and the like; the mass percentage of the penetrating agent can be 0.2%, 0.5%, 0.8%, 1%, 1.5% or 1.8% and the like; the mass percentage content of the pH regulator can be 1.5%, 2% or 2.5% and the like; the preservative can be 0.6%, 0.8% or 0.9% by mass.
In a second aspect, the present invention also provides a method for preparing the anti-aging composition according to the first aspect, the method comprising:
dispersing the formula amount of tea extract, watercress extract, field horsetail extract, nettle leaf extract, silybum marianum fruit extract, tetrahydro-methyl pyrimidine carboxylic acid and ergothioneine in water to obtain the anti-aging composition.
Preferably, the temperature of the dispersion is 40 ℃ to 45 ℃, for example, 41 ℃, 42 ℃, 43 ℃, 44 ℃ or the like.
As a preferred technical scheme of the invention, the preparation method comprises the following steps:
(1) adding humectant, penetrant and emulsifier into water, heating to 70-90 deg.C (such as 72 deg.C, 74 deg.C, 75 deg.C, 76 deg.C, 78 deg.C, 80 deg.C, 82 deg.C, 84 deg.C, 85 deg.C, 86 deg.C or 88 deg.C), stirring for dissolving, and maintaining for 30-35 min (such as 31min, 32min, 33min or 34 min);
(2) cooling to 40-45 deg.C (such as 41 deg.C, 42 deg.C, 43 deg.C or 44 deg.C), adding thickener and antiseptic, stirring, and adding pH regulator;
(4) cooling to 25-35 deg.C (such as 26 deg.C, 27 deg.C, 28 deg.C, 29 deg.C, 30 deg.C, 31 deg.C, 32 deg.C, 33 deg.C or 34 deg.C), adding folium Camelliae sinensis extract, herba Petasitis Dodecandri extract, herba Urticae Cannabinae leaf extract, herba Silybi Mariani fruit extract, tetrahydro-methylpyrimidine carboxylic acid, ergothioneine and skin conditioner, stirring for dissolving, and keeping the temperature for 10-15 min (such as 11min, 12min, 13min or 14 min) to obtain the antiaging composition.
In a third aspect, the present invention provides a cosmetic comprising the anti-aging composition according to the first aspect.
Preferably, the anti-aging composition in the cosmetic is 10 to 20% by mass, for example, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, or the like.
Preferably, the cosmetic comprises any one of a lotion, essence, emulsion, cream, eye cream or mask.
In a fourth aspect, the present invention also provides a use of the anti-aging composition according to the first aspect in the preparation of cosmetics.
The recitation of numerical ranges herein includes not only the above-recited values, but also any values between any of the above-recited numerical ranges not recited, and for brevity and clarity, is not intended to be exhaustive of the specific values encompassed within the range.
Compared with the prior art, the invention has the beneficial effects that:
(1) the anti-aging composition provided by the invention comprises a tea extract, a watercress flower/leaf/stem extract, an equisetum arvense extract, an isonettle leaf extract, a silybum marianum fruit extract, tetrahydro-methyl pyrimidine carboxylic acid and ergothioneine, can comprehensively solve the problem of skin aging from multiple angles of eliminating active oxygen, resisting oxidation, resisting saccharification, preventing pollution and the like, and can prevent skin aging; in the anti-aging composition, the effective components are matched with each other and have synergistic effect, so that a certain problem of aging is not solved singly, but various problems are solved comprehensively, and an excellent anti-aging effect is achieved;
(2) the DPPH free radical clearance rate of the anti-aging composition is 17.6-23.6%, the accumulation amount of ROS epidermal keratinocytes is 15.6-16.8%, the AEGs inhibition rate is 48.5-52.3%, and the anti-aging composition can effectively clear free radicals, inhibit the generation of ROS of human epidermal keratinocytes under a pollution condition, further protect skin cells from the pressure of environmental pollution, and simultaneously inhibit saccharification reaction, the cosmetics prepared from the anti-aging composition have excellent wrinkle removing and anti-aging effects, and the wrinkle depth of the prepared face cream is reduced to 18.6-20.6% after the prepared face cream is used for 30 days.
Detailed Description
The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.
In the following examples, the tea (CAMELLIASINENSIS) leaf extract is sold under the trade name "tea (CAMELLIASINENSIS)
Yuzhi tea, purchased from Nippon Wanshan pharmaceutical Co., Ltd;
the flower/leaf/stem extract of herba seu radix Dolichoris (NASTURTIUM OFFICINALE) and herba Equiseti Arvinsis (Equisetum)M ARVENSE) extract and Urtica DIOICA (Urtica DIOICA) leaf extract are available under the trade names
URB, available from Lipoid Kosmetik AG;
the Silybum MARIANUM (SILYBUM MARIANUM) fruit extract is available under the trade name Ameliox and is available from holstein;
the tetrahydro-methyl pyrimidine carboxylic acid is available under the trade name Ectoin from bitop company of Germany;
the ergothioneine is sold under the trade name THIOTAINE, available from NIKKOL, heliochemistry.
Example 1
The embodiment provides an anti-aging composition, which specifically comprises the following components:
the preparation method comprises the following steps:
(1) adding humectant, osmotic pressure regulator and emulsifier into water, heating to 60 deg.C, stirring for dissolving, and keeping the temperature for 35 min;
(2) cooling to 40 deg.C, adding folium Camelliae sinensis extract, herba Petasitis Dodecandri extract, herba Equiseti Arvinsis extract, Urtica dioica leaf extract, herba Silybi Mariani extract, tetrahydro-methyl pyrimidine carboxylic acid, ergothioneine and skin conditioner, stirring for dissolving, and keeping the temperature for 10 min;
(4) and cooling to 25 ℃, adding a thickening agent and a preservative, stirring, and adding a pH regulator to obtain the anti-aging composition.
Example 2
The embodiment provides an anti-aging composition, which specifically comprises the following components:
the preparation method is the same as that of example 1.
Example 3
The embodiment provides an anti-aging composition, which specifically comprises the following components:
the preparation method is the same as that of example 1.
Example 4
The difference from the example 1 is that the tea extract, the tetrahydro-methyl pyrimidine carboxylic acid and the ergothioneine in the example respectively have the following mass percentage contents: 2%, 1% and 6%; the remaining components and preparation were the same as in example 1.
Example 5
The difference from the example 1 is that the tea extract, the tetrahydro-methyl pyrimidine carboxylic acid and the ergothioneine in the example respectively have the following mass percentage contents: 2%, 4% and 5%; the remaining components and preparation were the same as in example 1.
Example 6
The difference from example 1 is that the mass percentages of the watercress extract, the equisetum arvense extract and the nettle leaf extract in the present example are respectively as follows: 1%, 1% and 4%; the remaining components and preparation were the same as in example 1.
Example 7
The difference from example 1 is that the mass percentages of the watercress extract, the equisetum arvense extract and the nettle leaf extract in the present example are respectively as follows: 2%, 3% and 1%; the remaining components and preparation were the same as in example 1.
Comparative example 1
The difference from example 1 is that this comparative example does not contain tea extract, and the content of ectoine carboxylic acid is increased to 6.5% and the content of ergothioneine is increased to 2.5%, and the remaining components and preparation method are the same as example 1.
Comparative example 2
The difference from example 1 is that in this comparative example, the tea leaf extract was replaced with the rose hip extract, and the remaining components and preparation method were the same as in example 1.
Comparative example 3
The difference from example 1 is that the comparative example does not contain silybum marianum fruit extract, and the content of tea extract is increased to 3%, the content of tetrahydro-methyl pyrimidine carboxylic acid is increased to 6%, the content of ergothioneine is increased to 3%, and the remaining components and preparation method are the same as example 1.
Comparative example 4
The difference from example 1 is that this comparative example does not contain tetrahydro-methyl pyrimidine carboxylic acid, and the content of tea extract is increased to 4.5%, the content of ergothioneine is increased to 4.5%, and the remaining components and preparation method are the same as example 1;
comparative example 5
The difference from example 1 is that the present comparative example does not contain ergothioneine, and the content of tea leaf extract is increased to 2.5% and the content of tetrahydro-methylpyrimidine carboxylic acid is increased to 6.5%, and the remaining components and preparation method are the same as example 1.
Performance test 1
Safety test (human skin patch test)
24 healthy subjects with no history of skin allergy between the ages of 25 and 50 were selected for patch testing in a closed patch test.
The specific spot sticking method comprises the following steps:
selecting a qualified spot tester, dropping 15 mu L of the anti-aging composition of the invention in the examples 1-7 and the comparative examples 1-5 into the spot tester, externally sticking a special adhesive tape on the back of a subject, sticking 12 spot testers on each subject, respectively sticking the anti-aging composition essence, removing the subject after 24 hours, observing skin reactions after 0.5, 6, 12, 24 and 48 hours after removal, and recording the results according to the skin reaction grading standard in the cosmetic hygiene Specification (2015 edition).
The classification criteria are shown in table 1 below:
TABLE 1
And (3) test results:
the results of the human skin patch test show that all the subjects pass the patch test and observe skin reactions at 0.5 hour, 6 hours, 12 hours, 24 hours and 48 hours respectively, wherein 0 case has adverse reactions such as erythema, pimple and blister, and the like, which indicates that the anti-aging compositions provided by the examples and the comparative examples in the invention have high safety and no stimulation.
Performance test 2
The anti-aging compositions of examples 1-7 and comparative examples 1-5 were tested for oxidation resistance, saccharification resistance, and contamination resistance, specifically as follows:
(1) oxidation resistance test
Evaluation of DPPH radical scavenging ability: the ability to scavenge DPPH (1, 1-diphenyl-2-trinitrophenylhydrazine) free radicals is to some extent the antioxidant ability of the reaction mass. The greater the free radical clearance rate, the stronger the antioxidant capacity and the stronger the anti-aging capacity. Therefore, the anti-aging effect of the anti-aging composition can be judged by studying the ability of the anti-aging composition to scavenge DPPH free radicals.
The samples were diluted with DMSO to 2% mass fraction of the sample to be tested and the following tests were performed:
adding 2.0mL of DPPH test solution with mass concentration of 45.8mg/L and a sample to be tested into a test tube, supplementing the total volume to 3mL by absolute ethyl alcohol, shaking up, reacting for 30min in a dark placeThe absorbance at 517nm of the sample was measured using a 1cm cuvette and recorded as A10(ii) a Adding 2mL of absolute ethyl alcohol and a sample to be detected with a corresponding volume into the test tube, supplementing the total volume to 3mL by the absolute ethyl alcohol, and recording the measured absorbance as A11(ii) a Adding 2mL of DPPH test solution and 1mL of absolute ethanol into the test tube, and recording the measured absorbance as A12Calculating the DPPH free radical clearance rate (Y') of the liquid to be detected;
the DPPH free radical clearance is calculated by the formula:
in the formula A10Absorbance value of system after removing DPPH for sample to be measured, A11Absorbance value of the System after eliminating DPPH for sample blank control, A12The absorbance values of the reaction system before adding no drug are shown in Table 2.
(2) Anti-contamination test
Detecting the content of Reactive Oxygen Species (ROS) in human keratinocytes treated with the particulate matter, specifically comprising the following steps:
cells were plated at 1.0X 105cells/mL were seeded onto a well plate at a density and after incubation for 2 hours at 37 deg.C, the cells were exposed to 100. mu.g/mL PM (particulate matter) and 2.5. mu.g/mL BaP. After incubation at 37 ℃ for 1 hour, detection and quantitation of fluorescence was performed using LS-5B spectrofluorescence (Perkinelmer, Waltham, MA, USA).
Since moderate and high doses of ROS induce apoptosis and even cause necrosis of cells through oxidative stress of cells, measuring the accumulation of ROS in epidermal keratinocytes reflects the anti-contamination ability of the test samples, and the test results are shown in Table 2.
(3) Anti-glycation assay
The advanced glycation end product removal experiment test is adopted for testing, and the following reagents are respectively prepared: 0.1mol/L NaOH solution, 0.05mol/L PBS phosphate buffer solution (pH7.4), 10mg/mL bovine serum albumin solution, 45mg/mL glucose solution, 0.25mmol/L aminoguanidine hydrochloride solution and 200mg/mL solution of a sample to be tested.
PBS phosphate buffer solution, bovine serum albumin solution and glucose solution are placed in a constant temperature and humidity incubator, then the sample solution to be tested and aminoguanidine hydrochloride solution are added, and incubation is carried out for 40h at the temperature of 60 ℃. And taking out each sample, detecting the samples by using an enzyme-linked immunosorbent assay, wherein the fluorescence excitation/emission wavelength is 370/440nm, and recording the fluorescence absorption value. 3 replicates of each sample were taken and the results averaged, where the inhibition of AEGs was calculated using the following formula:
wherein A isSample (I)Indicates the fluorescence intensity of the solution to which the test substance and glucose were added; a. theSample blankThe fluorescence intensity of the sample with the addition of the test substance and the fluorescence intensity of the sample without the addition of the glucose solution is shown; a. theNegative controlIndicating the fluorescence intensity of the glucose solution without the test substance; a. theSugar deficiency negative controlThis indicates the fluorescence intensity of the glucose solution without the test substance.
The results obtained are shown in table 2 below:
TABLE 2
The anti-aging composition provided by the invention has obvious antioxidant, anti-photoaging and anti-saccharification effects, the DPPH free radical clearance rate of the anti-aging composition is 17.6-23.6%, the ROS epidermal keratinocyte accumulation amount is 15.6-16.8%, and the AEGs inhibition rate is 48.5-52.3%, which shows that the anti-aging composition can effectively eliminate free radicals, inhibit the generation of ROS in human epidermal keratinocytes under pollution conditions, further protect skin cells from the pressure of environmental pollution, and inhibit saccharification reaction at the same time. Therefore, the anti-aging composition provided by the invention can realize anti-aging effect from multiple aspects, and enables the skin to be young and healthy.
Application example 1
The application example provides an all-dimensional anti-aging face cream.
Mixing the anti-aging composition provided in example 1 with a cream base to prepare an all-round anti-aging cream A, wherein the anti-aging composition accounts for 30% by mass;
the cream matrix is a basic skin care product without other effects, and comprises the following main components in percentage by mass: 3% of glycerol, 2% of butanediol, 1% of acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, 0.5% of behenyl alcohol, 0.5% of stearyl alcohol, 0.5% of PEG-60 glyceryl isostearate, 0.5% of PEG-5 glyceryl stearate, 0.02% of phenoxyethanol, 0.02% of potassium hydroxide, 0.5% of disodium EDTA and the balance of water.
Application example 2
The application example provides the all-round anti-aging toning lotion.
Mixing the anti-aging composition provided in example 1 with a lotion base to prepare an all-round anti-aging lotion, wherein the anti-aging composition accounts for 30% by mass;
the cosmetic water matrix is a basic skin care product without other effects, and comprises the following main components in percentage by mass: 3% glycerol, 2% disodium EDTA, 1% citric acid, 1% sodium citrate, 1.5% 1, 2-pentanediol, 0.1% polysorbate-20 and 0.02% phenoxyethanol, the balance being water.
Application example 3
The application example provides an all-dimensional anti-aging face cream.
Mixing the anti-aging composition provided in example 1 with an emulsion matrix to obtain an all-directional anti-aging emulsion, wherein the anti-aging composition accounts for 30% by mass;
the emulsion matrix is a basic skin care product without other effects, and comprises the following main components in percentage by mass: 3% glycerol, 2% butylene glycol, 2% disodium EDTA, 1% carbomer, 0.5% PEG-5 glyceryl stearate, 0.5% acrylic/C10-30 alkanol acrylate crosspolymer, 0.5% citric acid, 0.5% sodium citrate, 0.1% polysorbate-20, 0.1% stearyl alcohol, 0.1% xanthan gum, 0.02% potassium hydroxide, and 0.02% phenoxyethanol, with the balance being water.
Application examples 4 to 7
An all-round anti-aging cream was provided, which was different from application example 1 in that the anti-aging composition provided in example 1 was replaced with the anti-aging compositions provided in examples 4 to 7, to obtain anti-aging cream B (application example 4), cream C (application example 5), cream D (application example 6), and cream E (application example 7).
Application comparative examples 1 to 5
An anti-aging cream is provided, which is different from application example 1 in that the anti-aging composition provided in example 1 is replaced with the anti-aging compositions provided in comparative examples 1 to 5 to obtain anti-aging cream F (application comparative example 1), cream G (application comparative example 2), cream H (application comparative example 3), cream I (application comparative example 4) and cream J (application comparative example 5).
Performance test 3
Clinical test of crow's feet
200 female volunteers aged 35-50 years and showing wrinkles around the eyes were selected as the test subjects, and were randomly divided into 10 groups of 20 persons each.
The wrinkle mark of the tested part around the eyes of the subject is obtained by using silicone resin, and then the cream A-J is respectively coated on one side of the eye of the subject, and the cream substrate is coated on the other side of the eye of the subject, 2 times a day, 0.3g each time, and 30 days are continued.
Wrinkle imprints were taken at periocular test sites of subjects 15 days and 30 days after treatment, respectively, and wrinkle depth was measured using a confocal microscope. The percentage reduction in wrinkle depth after 15 days and 30 days of treatment was calculated and the results are shown in table 3 below.
TABLE 3
The results in the table show that the facial creams A to E provided by the invention can obviously reduce the wrinkle depth of users, can reduce the wrinkle depth by 7.3 to 8.1 percent when used for 15 days, can be used continuously, have better effect, and can reduce the wrinkle depth by 18.6 to 20.6 percent when used for 30 days.
The applicant declares that the above description is only a specific embodiment of the present invention, but the scope of the present invention is not limited thereto, and it should be understood by those skilled in the art that any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are within the scope and disclosure of the present invention.
Claims (10)
1. An anti-aging composition, comprising: tea leaf extract, watercress extract, horsetail extract, nettle leaf extract, silybum marianum fruit extract, tetrahydro-methyl pyrimidine carboxylic acid and ergothioneine.
2. The anti-aging composition as claimed in claim 1, wherein the mass ratio of the watercress extract, the equisetum arvense extract and the nettle leaf extract is 1 (0.5-1.5) to (1.5-3);
preferably, the mass ratio of the tea extract to the tetrahydro-methylpyrimidine carboxylic acid to the ergothioneine is 1 (2-5) to 0.8-2.
3. The anti-aging composition according to claim 1 or 2, further comprising a skin conditioner;
preferably, the skin conditioning agent comprises any one or a combination of at least two of vitamin E, carnosine, niacinamide, or sodium hyaluronate;
preferably, the anti-aging composition further comprises a humectant;
preferably, the humectant comprises any one or a combination of at least two of glycerol, ethanol or butylene glycol.
4. The anti-aging composition according to any one of claims 1 to 3, further comprising an emulsifier;
preferably, the anti-aging composition further comprises a penetrant;
preferably, the anti-aging composition further comprises a thickener;
preferably, the anti-aging composition further comprises a pH adjuster;
preferably, the anti-aging composition further comprises a preservative.
5. The anti-aging composition according to any one of claims 1 to 4, characterized by comprising, in mass percent:
1-10% of tea extract, 1-5% of watercress extract, 1-5% of field horsetail extract, 1-5% of nettle leaf extract, 3-10% of silybum marianum fruit extract, 1-10% of tetrahydro-methylpyrimidine carboxylic acid, 1-5% of ergothioneine, 0.1-5% of vitamin E, 0.1-3% of carnosine, 0.5-1% of hydrogenated lecithin, 0.5-1% of lecithin, 1-3% of thickening agent, 5-30% of humectant, 0.1-2% of penetrating agent, 1-3% of pH regulator, 0.5-1% of preservative and the balance of water.
6. A method for preparing the anti-aging composition according to any one of claims 1 to 5, wherein the method comprises the following steps:
dispersing the formula amount of tea extract, watercress extract, horsetail extract, nettle leaf extract, silybum marianum fruit extract, tetrahydro-methyl pyrimidine carboxylic acid and ergothioneine in water to obtain the anti-aging composition;
preferably, the temperature of the dispersion is 40 ℃ to 45 ℃.
7. The method of claim 6, comprising the steps of:
(1) adding the humectant, the penetrant and the emulsifier into water, heating to 70-90 ℃, stirring for dissolving, and keeping the temperature for 30-35 min;
(2) cooling to 40-45 ℃, adding a thickening agent and a preservative, stirring, and adding a pH regulator;
(4) cooling to 25-35 ℃, adding a tea extract, a watercress extract, an equisetum arvense extract, an isourtica leaf extract, a silybum marianum fruit extract, tetrahydro-methyl pyrimidine carboxylic acid, ergothioneine and a skin conditioner, stirring and dissolving, and keeping the temperature for 10-15 min to obtain the anti-aging composition.
8. A cosmetic comprising the anti-aging composition according to any one of claims 1 to 5.
9. The cosmetic according to claim 8, wherein the anti-aging composition is contained in the cosmetic in an amount of 10 to 20% by mass;
preferably, the cosmetic comprises any one of a lotion, essence, emulsion, cream, eye cream or mask.
10. Use of the anti-aging composition according to any one of claims 1 to 5 for the preparation of cosmetics.
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| CN115040429A (en) * | 2022-07-18 | 2022-09-13 | 华熙生物科技股份有限公司 | Anti-aging composition and application thereof |
| CN115282081A (en) * | 2022-07-05 | 2022-11-04 | 浙江东印化妆品有限公司 | Efficient whitening spot-fading and mark-repairing composition and application thereof |
| CN115554218A (en) * | 2022-11-01 | 2023-01-03 | 广州英凡妮生物科技有限公司 | Antioxidant and anti-sugar composition containing orchid extract and preparation method and application thereof |
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| DE102004050563A1 (en) * | 2004-10-15 | 2006-04-20 | Henkel Kgaa | Topical cosmetic and dermatological composition containing alkylurea and other active agent, useful for treating e.g. ageing of the skin and pigmentation disorders |
| EP3090781A1 (en) * | 2015-05-08 | 2016-11-09 | Susanne Kaufmann | Anti-age composition with plant extracts |
| CN106420472A (en) * | 2016-10-25 | 2017-02-22 | 广州智媛生物科技有限公司 | Multifunctional anti-wrinkle skincare product composition and preparation method thereof |
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| DE102004050563A1 (en) * | 2004-10-15 | 2006-04-20 | Henkel Kgaa | Topical cosmetic and dermatological composition containing alkylurea and other active agent, useful for treating e.g. ageing of the skin and pigmentation disorders |
| EP3090781A1 (en) * | 2015-05-08 | 2016-11-09 | Susanne Kaufmann | Anti-age composition with plant extracts |
| CN106420472A (en) * | 2016-10-25 | 2017-02-22 | 广州智媛生物科技有限公司 | Multifunctional anti-wrinkle skincare product composition and preparation method thereof |
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| CN115282081A (en) * | 2022-07-05 | 2022-11-04 | 浙江东印化妆品有限公司 | Efficient whitening spot-fading and mark-repairing composition and application thereof |
| CN115040429A (en) * | 2022-07-18 | 2022-09-13 | 华熙生物科技股份有限公司 | Anti-aging composition and application thereof |
| CN115554218A (en) * | 2022-11-01 | 2023-01-03 | 广州英凡妮生物科技有限公司 | Antioxidant and anti-sugar composition containing orchid extract and preparation method and application thereof |
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