CN113367088A - Method for regulating growth immunity of freshwater shrimps by medium-short chain fatty acid - Google Patents
Method for regulating growth immunity of freshwater shrimps by medium-short chain fatty acid Download PDFInfo
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- CN113367088A CN113367088A CN202110471946.6A CN202110471946A CN113367088A CN 113367088 A CN113367088 A CN 113367088A CN 202110471946 A CN202110471946 A CN 202110471946A CN 113367088 A CN113367088 A CN 113367088A
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- fatty acid
- medium
- chain fatty
- short chain
- freshwater shrimps
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- 239000013505 freshwater Substances 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 16
- 230000036039 immunity Effects 0.000 title claims abstract description 13
- 230000001105 regulatory effect Effects 0.000 title claims abstract description 9
- 241000143060 Americamysis bahia Species 0.000 title claims abstract 15
- 241000607626 Vibrio cholerae Species 0.000 claims abstract description 12
- 244000052616 bacterial pathogen Species 0.000 claims abstract description 12
- 229940118696 vibrio cholerae Drugs 0.000 claims abstract description 11
- 230000004083 survival effect Effects 0.000 claims abstract description 10
- 230000004584 weight gain Effects 0.000 claims abstract description 10
- 235000019786 weight gain Nutrition 0.000 claims abstract description 10
- 239000000654 additive Substances 0.000 claims abstract description 3
- 230000000996 additive effect Effects 0.000 claims abstract description 3
- 241000238557 Decapoda Species 0.000 claims description 28
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims description 10
- 235000019733 Fish meal Nutrition 0.000 claims description 7
- 239000004467 fishmeal Substances 0.000 claims description 7
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims description 5
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 claims description 5
- 235000019743 Choline chloride Nutrition 0.000 claims description 5
- UPEZCKBFRMILAV-JNEQICEOSA-N Ecdysone Natural products O=C1[C@H]2[C@@](C)([C@@H]3C([C@@]4(O)[C@@](C)([C@H]([C@H]([C@@H](O)CCC(O)(C)C)C)CC4)CC3)=C1)C[C@H](O)[C@H](O)C2 UPEZCKBFRMILAV-JNEQICEOSA-N 0.000 claims description 5
- 235000019779 Rapeseed Meal Nutrition 0.000 claims description 5
- DFPOZTRSOAQFIK-UHFFFAOYSA-N S,S-dimethyl-beta-propiothetin Chemical compound C[S+](C)CCC([O-])=O DFPOZTRSOAQFIK-UHFFFAOYSA-N 0.000 claims description 5
- 235000019764 Soybean Meal Nutrition 0.000 claims description 5
- UPEZCKBFRMILAV-UHFFFAOYSA-N alpha-Ecdysone Natural products C1C(O)C(O)CC2(C)C(CCC3(C(C(C(O)CCC(C)(C)O)C)CCC33O)C)C3=CC(=O)C21 UPEZCKBFRMILAV-UHFFFAOYSA-N 0.000 claims description 5
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 5
- 239000001506 calcium phosphate Substances 0.000 claims description 5
- 235000012000 cholesterol Nutrition 0.000 claims description 5
- 229960003178 choline chloride Drugs 0.000 claims description 5
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 claims description 5
- UPEZCKBFRMILAV-JMZLNJERSA-N ecdysone Chemical compound C1[C@@H](O)[C@@H](O)C[C@]2(C)[C@@H](CC[C@@]3([C@@H]([C@@H]([C@H](O)CCC(C)(C)O)C)CC[C@]33O)C)C3=CC(=O)[C@@H]21 UPEZCKBFRMILAV-JMZLNJERSA-N 0.000 claims description 5
- 235000021323 fish oil Nutrition 0.000 claims description 5
- 235000013312 flour Nutrition 0.000 claims description 5
- 235000012054 meals Nutrition 0.000 claims description 5
- 229910000150 monocalcium phosphate Inorganic materials 0.000 claims description 5
- 235000019691 monocalcium phosphate Nutrition 0.000 claims description 5
- 239000004456 rapeseed meal Substances 0.000 claims description 5
- 239000004455 soybean meal Substances 0.000 claims description 5
- 241000238371 Sepiidae Species 0.000 claims description 4
- 238000009395 breeding Methods 0.000 claims description 3
- 230000001488 breeding effect Effects 0.000 claims description 3
- 229940083466 soybean lecithin Drugs 0.000 claims description 3
- 235000012424 soybean oil Nutrition 0.000 claims description 3
- 239000003549 soybean oil Substances 0.000 claims description 3
- LDVVTQMJQSCDMK-UHFFFAOYSA-N 1,3-dihydroxypropan-2-yl formate Chemical compound OCC(CO)OC=O LDVVTQMJQSCDMK-UHFFFAOYSA-N 0.000 claims 1
- 241000238366 Cephalopoda Species 0.000 claims 1
- 238000009472 formulation Methods 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 244000052769 pathogen Species 0.000 claims 1
- 230000001717 pathogenic effect Effects 0.000 claims 1
- 238000009360 aquaculture Methods 0.000 abstract description 2
- 244000144974 aquaculture Species 0.000 abstract description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- ARIWANIATODDMH-UHFFFAOYSA-N Lauric acid monoglyceride Natural products CCCCCCCCCCCC(=O)OCC(O)CO ARIWANIATODDMH-UHFFFAOYSA-N 0.000 description 8
- POULHZVOKOAJMA-UHFFFAOYSA-N dodecanoic acid Chemical compound CCCCCCCCCCCC(O)=O POULHZVOKOAJMA-UHFFFAOYSA-N 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 241000058338 Macrobrachium nipponense Species 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 239000005639 Lauric acid Substances 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 description 2
- 229940022405 astaxanthin Drugs 0.000 description 2
- 235000013793 astaxanthin Nutrition 0.000 description 2
- 239000001168 astaxanthin Substances 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000008347 soybean phospholipid Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 239000005711 Benzoic acid Substances 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000701022 Cytomegalovirus Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 235000010233 benzoic acid Nutrition 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000032050 esterification Effects 0.000 description 1
- 238000005886 esterification reaction Methods 0.000 description 1
- 125000005456 glyceride group Chemical group 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- -1 p-hydroxybenzoate ester Chemical class 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000036186 satiety Effects 0.000 description 1
- 235000019627 satiety Nutrition 0.000 description 1
- 150000004666 short chain fatty acids Chemical class 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Biotechnology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Botany (AREA)
- Mycology (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Inorganic Chemistry (AREA)
- Biomedical Technology (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention relates to the technical field of aquaculture, in particular to a method for regulating the growth immunity of freshwater shrimps by medium-short chain fatty acid, which comprises the steps of adopting a culture barrel to culture the freshwater shrimps, adding the medium-short chain fatty acid as an additive into basic bait to feed the freshwater shrimps, collecting data of average initial weight, average final weight, initial tail number and tail number of the freshwater shrimps before and after the freshwater shrimps are fed by the basic bait added with the medium-short chain fatty acid, calculating the weight gain rate, the specific growth rate and the survival rate, and remarkably improving the resistance of the medium-short chain fatty acid to pathogenic bacteria, namely non-01 vibrio cholerae on the premise of keeping the weight gain rate of the freshwater shrimps by observing the survival condition for 96 hours through injecting the pathogenic bacteria, thereby regulating the growth immunity of the freshwater shrimps.
Description
Technical Field
The invention relates to the technical field of aquaculture, in particular to a method for regulating the growth immunity of freshwater shrimps by using medium-short chain fatty acids.
Background
The freshwater shrimp is also called as macrobrachium nipponensis, is rich in nutrition, is rich in magnesium, phosphorus, calcium, astaxanthin and other trace elements, is particularly important for supplementing and influencing children and pregnant women, has obvious effect on eliminating time difference by the astaxanthin, and is suitable for people of all ages. The fresh water aquatic product is widely pursued in Jiang, Zhe and Shanghai areas, but the price of the fresh water aquatic product is always high and is about 100 yuan per jin, so that the fresh water aquatic product is a luxury fresh water aquatic product.
And the large-scale artificial culture easily causes frequent outbreaks of aquatic diseases, and causes a large amount of economic losses. The discovery and research of the drug with no residue and remarkable disease resistance becomes a hotspot in the research field, and the times of 'no resistance' and 'resistance replacement' have come.
Disclosure of Invention
The invention aims to overcome the technical problems and provides a medium-short chain fatty acid for regulating the growth immunity of freshwater shrimps, aiming at solving the problems in the background technology.
The technical scheme for solving the technical problems is as follows:
the macrobrachium nipponense feed is characterized in that lauric acid monoglyceride is used as an additive and added into basic bait to feed macrobrachium nipponense, and the lauric acid monoglyceride is directly synthesized by esterification of lauric acid and glycerol and has 15 carbon atoms.
The basic bait is low fish meal bait, and part of live shrimps are reserved in each breeding barrel to inject non-01 vibrio cholerae pathogenic bacteria to observe the survival condition for 96 hours. The low fish meal bait comprises, but is not limited to, fish meal, soybean meal, rapeseed meal, shrimp meal, cuttlefish paste, flour, fish oil, soybean lecithin, cholesterol, monocalcium phosphate, premix, Vc, choline chloride, ecdysone and DMPT.
Further, the medium-short chain fatty acid is added in an amount of 0.15% by mass.
Further, live shrimp was injected with a concentration of 10 to non-01 V.cholerae pathogenic bacteria8One per ml.
The breeding barrel is provided with the same number of hiding pipes and the false aquatic plants as shielding objects, and the shielding objects are added after being sterilized. The breed bucket include:
a water inlet; the water inlet is communicated with the water inlet pipe;
a water outlet; the water outlet is communicated with a water drainage pipe;
a blocking member for blocking zooplankton from entering the drain pipe; the blocking piece is of a spherical net structure and covers the outer side of the drain pipe.
The invention has the beneficial effects that:
the invention develops a method for regulating the growth immunity of freshwater shrimps by medium-short chain fatty acid, wherein the medium-short chain fatty acid, namely the short chain fatty acid and the medium-short chain fatty acid are collectively called as an important functional substance of biological energy and also have the functions of regulating the intestinal tract of an organism, resisting bacteria and viruses, and the lauric acid monoglyceride in the medium-short chain fatty acid, namely the lauric acid monoglyceride is synthesized by lauric acid and glyceride, has the function of supplying energy to regulate the intestinal tract, simultaneously has the functions of inhibiting cytomegalovirus, herpes virus, cold virus and a large number of bacteria and protozoa, and has the antibacterial effect which is not influenced by pH, so the medium-short chain fatty acid is superior to chemical substances such as benzoic acid, p-hydroxybenzoate ester and the like. And the preposed chemicals are easy to obtain and cheap, and the synthesis process is relatively simple, so that the production cost is low.
According to the invention, the lauric acid monoglyceride is added into the basic bait to feed the freshwater shrimps, and through the data analysis of the weight gain rate, the specific growth rate and the survival rate, the lauric acid monoglyceride added into the bait is verified to be beneficial to improving the immunity of the freshwater shrimps to non-01 vibrio cholerae pathogenic bacteria, namely, the medium-short chain fatty acid can enhance the immunity of the freshwater shrimps under the condition of keeping the existing weight gain rate of the freshwater shrimps.
Detailed Description
The present invention will be described in further detail with reference to specific embodiments.
Example 1:
1) weighing the feed raw materials according to the following mass formula:
0.15% of lauric acid monoglyceride, 30% of fish meal, 22% of soybean meal, 10% of rapeseed meal, 6% of shrimp meal, 3% of cuttle-fish paste, 1.5% of fish oil, 1.5% of soybean oil, 1% of soybean phospholipid, 0.5% of cholesterol, 2% of monocalcium phosphate, 1% of premix, 0.5% of Vc, 0.5% of choline chloride, 0.02% of ecdysone, 0.05% of DMPT and 20.28% of flour.
2) Pulverizing the above feed materials with a pulverizer, sieving with a 40 mesh sieve, adding water, mixing with a blender, extruding into strips with diameter of 1mm with a meat grinder, air drying at room temperature in the shade, pulverizing with a pulverizer into granules, and placing in a refrigerator at-20 deg.C for use.
3) Raising
Selecting active shrimp seedlings with regular specifications as test shrimps, putting 50 shrimps into each barrel, and training for one week; the test barrel is a small-sized cultivation barrel, the diameter of the cultivation barrel is 1.5m, the water depth is 20-40 cm, the disinfected and sterilized false aquatic weeds and the sheltering pipe are placed in the cultivation barrel to provide shelters for shrimps, the oxygenation rod is added, and oxygen is supplied for 24 hours. The test water is tap water, and during the culture period, the water temperature is 16-25 ℃, the dissolved oxygen is 8-9 mg/L, the ammonia nitrogen is 0.15mg/L, the nitrite is 0.0005-0.01 mg/L, and the pH is 7.1-7.4.
Feeding for 3 times a day, respectively 8:30, 12:30 and 17: 30; and (3) adopting a feeding strategy of satiety, collecting excrement and residual bait 1-2 h after feeding every time, and changing water every three days, wherein the water changing amount is 30%.
4) Analysis of growth conditions
Weighing and counting respectively on day 1 and day 52, calculating weight gain rate, specific growth rate and survival rate, leaving part of live shrimp in the cultivation barrel, injecting pathogenic bacteria non-01 Vibrio cholerae to observe survival condition for 96h, wherein the concentration of pathogenic bacteria of non-01 Vibrio cholerae injected into live shrimp is 108One per ml.
Wherein the weight gain ratio (WG,%) (average final weight-average initial weight)/average initial weight × 100
Specific Growth Rate (SGR) (last average weight of Ln-first average weight of Ln)/test day × 100
Survival (SR,%) end/start mantissa × 100.
Comparative example 1:
the difference from the example 1 is that the feed raw material formula is as follows:
30% of fish meal, 22% of soybean meal, 10% of rapeseed meal, 6% of shrimp meal, 3% of cuttlefish paste, 1.5% of fish oil, 1.5% of soybean oil, 1% of soybean phospholipid, 0.5% of cholesterol, 2% of monocalcium phosphate, 1% of premix, 0.5% of Vc, 0.5% of choline chloride, 0.02% of ecdysone, 0.05% of DMPT and 20.43% of flour.
The embodiments of example 1 and comparative example 1 were performed 3 times in parallel, respectively, and growth conditions were analyzed according to the formula, and one-way anova results of the entire samples were performed as shown in table 1 below.
TABLE 1 analysis results of growth conditions
The 96h survival observed with the injection of pathogenic bacteria non 01 Vibrio cholerae is shown in Table 2 below.
TABLE 296H mortality Change in freshwater shrimps
As can be seen from tables 1 and 2, the shrimp fed by the method of example 1 had significantly better growth than that of comparative example 1; according to the method, the addition of the lauric acid monoglyceride in the bait is beneficial to improving the immunity of the freshwater shrimps to non-01 vibrio cholerae pathogenic bacteria, namely, the medium-short chain fatty acid can enhance the immunity of the freshwater shrimps under the condition of keeping the existing weight gain rate of the freshwater shrimps.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, and all simple modifications and equivalent variations of the above embodiment according to the present invention are within the scope of the present invention.
Claims (7)
1. A method for regulating the growth immunity of freshwater shrimps by medium-short chain fatty acid is characterized by comprising the following steps:
the method is characterized in that a breeding barrel is adopted to breed the freshwater shrimps, medium-short chain fatty acid is used as an additive and added into basic bait to feed the freshwater shrimps, and the medium-short chain fatty acid remarkably improves the resistance to pathogenic bacteria, namely non-01 vibrio cholerae on the premise of keeping the weight gain rate of the freshwater shrimps, so that the growth immunity of the freshwater shrimps is adjusted.
2. The method as claimed in claim 1, wherein the average initial weight, average final weight, initial mantissa and end number data of the freshwater shrimps before and after being fed with the basic bait added with medium-short chain fatty acid are collected, the weight gain rate, the specific growth rate and the survival rate are calculated, and the medium-short chain fatty acid is proved to be capable of remarkably improving the resistance to the pathogenic bacteria non-01 vibrio cholerae on the premise of keeping the weight gain rate of the freshwater shrimps by injecting the pathogenic bacteria non-01 vibrio cholerae and observing the survival condition for 96 h.
3. The method of claim 1, wherein the medium-short chain fatty acid is lauric monoglyceride.
4. The method of claim 1, wherein the basic bait comprises fish meal, soybean meal, rapeseed meal, shrimp meal, cuttlefish paste, fish oil, soybean lecithin, cholesterol, monocalcium phosphate, premix, Vc, choline chloride, ecdysone, DMPT, and flour.
5. The method of claim 4, wherein the basic bait formulation is 30% fish meal, 22% soybean meal, 10% rapeseed meal, 6% shrimp meal, 3% squid extract, 1.5% fish oil, 1.5% soybean oil, 1% soybean lecithin, 0.5% cholesterol, 2% monocalcium phosphate, 1% premix, 0.5% Vc, 0.5% choline chloride, 0.02% ecdysone, 0.05% DMPT, and the balance flour.
6. The method according to claim 1, wherein the medium-short chain fatty acid is added in an amount of 0.15% by mass.
7. The method of claim 2, wherein the live shrimp is injected with a concentration of non-01 vibrio cholerae pathogen of 108One per ml.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107518180A (en) * | 2017-09-06 | 2017-12-29 | 安徽蓝田特种龟鳖有限公司 | A kind of nutritious testudinate aquatic feeds |
| CN109363002A (en) * | 2018-12-13 | 2019-02-22 | 浙江农林大学 | A kind of freshwater crayfish feed stuff additive and its application |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107518180A (en) * | 2017-09-06 | 2017-12-29 | 安徽蓝田特种龟鳖有限公司 | A kind of nutritious testudinate aquatic feeds |
| CN109363002A (en) * | 2018-12-13 | 2019-02-22 | 浙江农林大学 | A kind of freshwater crayfish feed stuff additive and its application |
Non-Patent Citations (1)
| Title |
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| JAE-IL LEE 等: "Influence of Acetic Acid ,Lauric Acid and Monolaurine Treatments on Survival of Vibrio cholerae in Refrigerated Flatfish", 《KOREAN J. FOOD & NUTR.》 * |
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