CN113355225B - Microorganism screening and culturing process for animal husbandry - Google Patents
Microorganism screening and culturing process for animal husbandry Download PDFInfo
- Publication number
- CN113355225B CN113355225B CN202110636641.6A CN202110636641A CN113355225B CN 113355225 B CN113355225 B CN 113355225B CN 202110636641 A CN202110636641 A CN 202110636641A CN 113355225 B CN113355225 B CN 113355225B
- Authority
- CN
- China
- Prior art keywords
- screening
- culture
- cylinder
- humidifying
- material taking
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
Abstract
The invention discloses a microbial screening and culturing process for animal husbandry, which comprises a base, a culturing mechanism, a condition adjusting mechanism and a material taking, diluting and screening system, wherein the culturing mechanism is arranged on the base; the base is the installation platform of cultivateing mechanism, condition adjustment mechanism, getting material dilution screening system. The material taking, diluting and screening system comprises a material taking mechanism, a screening mechanism and an extraction cylinder. The incubator can adjust the temperature and the humidity in the incubator at any time, and provides a proper environment when the base number of microorganisms in the incubator needs to be improved; the design of the water tank can isolate the pollution to the sterile water caused by the contact and the backflow of the sterile water for the laboratory and the air; the material taking, diluting and screening system can automatically complete gradient dilution, and a diluted sample is placed on a culture medium; the working efficiency is improved, the labor force is saved, and the culture quality is improved.
Description
Technical Field
The invention relates to the technical field of biological culture, in particular to a microbial screening and culturing process for animal husbandry.
Background
In the field of biological instruments, when microorganisms are cultured, harsh experimental environments and complex operation flows are needed, the experimental instruments among all the flows are scattered, when samples are cultured, the environmental temperature, the humidity and the like need to be repeatedly monitored for many times, adjustment needs to be carried out when the environmental temperature changes, if the adjustment is not in time, the whole experiment can even fail, in the operation of the experiment, the operation is repeated monotonously in many times, and the manual operation easily influences the experiment effect because of the failure of a certain link. Application No.: CN201820482955.9 discloses an agricultural microorganism culture apparatus, which is used to manually complete sampling, sealing and other operations, and does not substantially reduce the workload, and the manual operation is easy to cause culture medium pollution. The invention can ensure that the culture medium completes the environmental adjustment in the incubator in a closed environment and fully automatically performs the later stage gradient dilution action.
Disclosure of Invention
Aiming at the technical problem, the invention provides a microbial screening and culturing process for animal husbandry, which comprises the following steps: putting a sample into a culture dish of a culture mechanism, adding water in a water tank on a base into the culture dish, adjusting the humidity and the temperature required by the culture dish by a condition adjusting mechanism during culture, controlling the humidity adjustment by a closed cylinder of the culture mechanism, and arranging a sealing cover on the culture mechanism to facilitate the examination of the culture dish; after the culture is finished, a material taking mechanism of the material taking dilution screening system is matched with an extraction cylinder to extract a sample, a plurality of test tubes are arranged on the material taking dilution screening system, the screening mechanism of the material taking dilution screening system releases the test solution sucked by the last test tube, the operation is repeated for several times, and finally the test solution is dropped on an end observation dish; the condition can be observed and test in the test tube during this period, if discover wherein certain test tube can't satisfy the requirement, screening mechanism will wholly get the material and dilute the horizontal rotation of screening system, avoid the test tube that has the problem, accomplish the screening and cultivate.
Further, the base is a mounting platform of the culture mechanism, the condition adjusting mechanism and the material taking, diluting and screening system; the base includes support, water tank, and water tank fixed mounting is on the support, and the inside top of water tank is provided with the filter screen, and the water tank side is provided with two water pipes, is provided with the humidification valve respectively on two water pipes and cultivates the valve.
Further, the culture mechanism comprises an incubator, a closed cylinder and a culture dish; the rotary mounting has the sealed cowling on the incubator, and sealed cylinder fixed mounting is in the side of incubator, and sealed cylinder end fixing has the shutoff board, is provided with the hole of humidifying on the incubator, and incubator fixed mounting has the cultivation slide rail, and slidable mounting has the culture dish on the cultivation slide rail.
Further, the condition adjusting mechanism comprises a humidifying fan and a heating pipe; the humidifying fan is fixedly arranged at a humidifying hole on the incubator, and the heating pipe is fixedly arranged at the bottom of the bracket and is arranged right below the culture mechanism; a humidifying core is arranged between the humidifying fan and the humidifying hole, the humidifying core is connected with a water pipe with a humidifying valve on the water tank, and the heating pipe is fixedly installed at the bottom of the support.
Further, get material and dilute the screening system and include feeding agencies, screening mechanism, an extraction section of thick bamboo, feeding agencies fixed mounting is on the support, and screening mechanism sets up in feeding agencies's below, and an extraction section of thick bamboo fixed mounting is provided with the test tube and observes the ware on the screening mechanism.
Furthermore, the material taking mechanism comprises a material taking slide rail and a pen-shaped air cylinder; get material slide rail fixed mounting on the support, get material slide rail slidable mounting and have the slider, connecting plate fixed mounting is on the slider, and the tip of stroke-shaped cylinder passes through connecting plate fixed mounting on the slider.
Furthermore, the screening mechanism comprises an upper position cylinder, a lower position cylinder and a screening frame; the screening frame is rotatably installed on a stretching seat below the upper position air cylinder and the lower position air cylinder, the screening frame penetrates through the motor base and is fixedly installed with the transmission gear shaft, the upper position air cylinder and the lower position air cylinder are fixedly installed at the bottom of the connecting plate, the stretching seat is fixedly installed at the end portion of the upper position air cylinder and the lower position air cylinder, the transmission gear shaft is rotatably installed on the motor base, the gear end of the transmission gear shaft is meshed with the power gear to rotate, and the power gear is fixedly installed with the motor.
Further, the extraction cylinder comprises a sliding frame and a suction pipe; the sliding frame is slidably mounted on the suction pipe, the piston lead screw is rotatably mounted on the sliding frame, the end part of the piston lead screw is fixedly provided with the piston, the piston lead screw is rotatably mounted on the sliding frame and the nut seat, the nut seat is fixedly mounted on the suction pipe, the piston penetrates through the nut seat and is rotatably mounted at the bottom of the piston lead screw, and the piston is arranged inside the suction pipe.
Compared with the prior art, the invention has the beneficial effects that: (1) the incubator can adjust the temperature and the humidity in the incubator at any time, and provides a proper environment when the base number of microorganisms in the incubator needs to be improved; (2) the design of the water tank can isolate the pollution to the sterile water caused by the contact and the backflow of the sterile water for the laboratory and the air; (3) the material taking, diluting and screening system can automatically complete gradient dilution, and a diluted sample is placed on a culture medium; (4) the working efficiency is improved, the labor force is saved, and the culture quality is improved.
Drawings
FIG. 1 is an overall schematic view of the present invention.
FIG. 2 is a schematic view of a base of the present invention.
FIG. 3 is a schematic view of a culture apparatus according to the present invention.
FIG. 4 is a schematic view of a conditioning mechanism according to the present invention.
Fig. 5 is a schematic view of a reject dilution screening system according to the present invention.
Fig. 6 is a schematic view of a material taking mechanism according to the present invention.
FIG. 7 is a schematic view of a screening mechanism of the present invention.
Fig. 8 is a schematic view of an extraction cartridge of the present invention.
Detailed Description
The present invention will be further described with reference to specific examples, which are illustrative of the invention and are not to be construed as limiting the invention.
Example (b): the screening and culturing process of microorganisms for animal husbandry as shown in FIG. 1, FIG. 2, FIG. 3, FIG. 4, FIG. 5, FIG. 6, FIG. 7 and FIG. 8 comprises the following steps: putting a sample into a culture dish of a culture mechanism, adding water in a water tank on a base into the culture dish, adjusting the humidity and the temperature required by the culture dish by a condition adjusting mechanism during culture, controlling the humidity adjustment by a closed cylinder of the culture mechanism, and arranging a sealing cover on the culture mechanism to facilitate the examination of the culture dish; after the culture is finished, a material taking mechanism of the material taking dilution screening system is matched with an extraction cylinder to extract a sample, a plurality of test tubes are arranged on the material taking dilution screening system, the screening mechanism of the material taking dilution screening system releases the test solution sucked by the last test tube, the operation is repeated for several times, and finally the test solution is dropped on an end observation dish; the condition can be observed and test in the test tube during this period, if discover wherein certain test tube can't satisfy the requirement, screening mechanism will wholly get the material and dilute the horizontal rotation of screening system, avoid the test tube that has the problem, accomplish the screening and cultivate.
As shown in fig. 2: the base 1 comprises a support 101, a water tank 102, a filter screen 103, a humidifying valve 104 and a culturing valve 105, the water tank 102 is fixedly mounted on the support 101, the filter screen 103 is arranged at the top end inside the water tank 102, two water pipes are arranged on the side face of the water tank 102, and the humidifying valve 104 and the culturing valve 105 are arranged on the two water pipes respectively.
As shown in fig. 3: the culture mechanism 2 comprises an incubator 201, a sealing cover 202, a sealing cylinder 203, a plugging plate 204, a humidifying hole 205, a culture dish 206 and a culture slide rail 207; the incubator 201 comprises an incubator first plate 20101, an incubator second plate 20102, an incubator three plate 20103, an incubator four plate 20104 and an incubator five plate 20105; the sealing cover 202 is rotatably installed on a second plate 20102 of the incubator, the closed cylinder 203 is fixedly installed on a fourth plate 20104 of the incubator, the plugging plate 204 is fixed at the end part of the closed cylinder 203, the first plate 20101 of the incubator is provided with a humidifying hole 205, the three plates 20103 of the incubator are fixedly installed with a culture slide rail 207, and the culture dish 206 is slidably installed on the culture slide rail 207.
As shown in fig. 4: the condition adjusting mechanism 3 comprises a humidifying fan 301, a humidifying core 302 and a heating pipe 303; the humidifying fan 301 is fixedly installed at the humidifying hole 205 on the first plate 20101 of the incubator, the humidifying core 302 is arranged between the humidifying fan 301 and the humidifying hole 205, and the heating pipe 303 is fixedly installed at the bottom of the support 101 and is arranged right below the culture mechanism 2.
As shown in fig. 5: the material taking, diluting and screening system 4 comprises a material taking mechanism 401, a screening mechanism 402, an extraction cylinder 403, a test tube 404 and an observation dish 405, wherein the material taking mechanism 401 is fixedly installed on the support 101, the screening mechanism 402 is arranged below the material taking mechanism 401, and the extraction cylinder 403 is fixedly installed on the screening mechanism 402.
As shown in fig. 6: the material taking mechanism 401 comprises a material taking slide rail 40101, a slide block 40102, a connecting plate 40103, a pen-shaped air cylinder 40104 and an air cylinder seat 40105; get material slide rail 40101 fixed mounting on support 101, slider 40102 slidable mounting gets on material slide rail 40101, connecting plate 40103 fixed mounting is on slider 40102, pen-shaped cylinder 40104 fixed mounting is on cylinder block 40105, cylinder block 40105 fixed mounting is on support 101, pen-shaped cylinder 40104's tip and connecting plate 40103 fixed connection.
As shown in fig. 7: the screening mechanism 402 comprises an upper position cylinder 40201, a lower position cylinder 40202, a tension seat 40202, a screening rack 40203, a test tube rack 40204, a motor seat 40205, a transmission gear shaft 40206, a power gear 40207 and a motor 40208; the upper and lower position cylinder 40201 is fixedly arranged at the bottom of the connecting plate 40103, the stretching base 40202 is fixedly arranged at the end part of the upper and lower position cylinder 40201, the screening frame 40203 passes through the motor base 40205 to be fixedly arranged with the transmission gear shaft 40206, the motor base 40205 is fixedly arranged with the stretching base 40202, the transmission gear shaft 40206 is rotatably arranged on the motor base 40205, the gear end of the transmission gear shaft 40206 is meshed with the power gear 40207 to rotate, the power gear 40207 passes through the bottom of the motor base 40205 to be fixedly arranged with the motor 40208, and the test tube 404 and the observation vessel 405 are arranged on the test tube rack 40204.
As shown in fig. 8: the extraction cylinder 403 comprises an extraction motor 40301, a sliding frame 40302, a piston lead screw 40303, a nut seat 40304, a suction pipe 40305 and a piston 40306; the extraction motor 40301 is fixedly arranged on a sliding frame 40302, the sliding frame 40302 is slidably arranged on a suction tube 40305, the piston screw 40303 is rotatably arranged on the sliding frame 40302 and a nut seat 40304, the nut seat 40304 is fixedly arranged on the suction tube 40305, the piston 40306 penetrates through the nut seat 40304 and is rotatably arranged at the bottom of the piston screw 40303, and the piston 40306 is arranged inside the suction tube 40305.
The working principle is as follows: adding water into a water tank 102, closing a water tank cover of the water tank 102, putting a sample into a culture dish 206, opening a culture valve 105 of a culture box 201, allowing sterile water to enter the culture dish to prepare for microbial culture, heating a heating pipe 303 to heat the culture box 201 if the temperature is required during culture, humidifying the culture box 201 if the temperature is required, contracting a closed cylinder 203, opening a humidifying channel, opening a humidifying valve 104, adding water into a humidifying core of a culture mechanism 208, closing, opening a humidifying fan of the culture dish 206, blowing air into the culture box 201, humidifying the culture box 201, starting the closed cylinder 203 to drive a blocking plate 204 to close the humidifying channel after the humidification is finished, opening a sealing cover 202 if the culture dish 206 is required to be checked in the middle of culture, pulling out the culture dish 206, pushing back to close the sealing cover 202 after checking, starting a pen-shaped cylinder 40104 to extend an extraction part into the culture box 201, starting an up-down position cylinder 40201, the extraction part extends into a culture dish 206, a motor 40208 is started, a piston screw 40303 is driven to rotate to pull a piston 40306 to extract culture solution, a pen-shaped air cylinder 40104 is utilized to contract, an upper position air cylinder 40201 and a lower position air cylinder 40201 extend out, the test solution sucked by the previous test tube 404 is released, action circulation is carried out for several times, finally, the test solution is dripped on a tail end observation dish 405, the conditions in the test tube 404 can be observed and the test can be carried out in the test tube 404 in the period, if one test tube 404 cannot meet the requirements, the motor 40208 at the lower end is started to drive a power gear 40207 and a transmission gear shaft 40206 to rotate in a matched mode, the whole material taking, diluting and screening system 4 transversely rotates to avoid the test tube 404 with problems, and screening culture is completed.
Claims (8)
1. A method for screening and culturing microorganisms for animal husbandry is characterized in that: which comprises the following steps: putting a sample into a culture dish of a culture mechanism, adding water in a water tank on a base into the culture dish, adjusting the humidity in the culture dish by a wet fan and a humidifying core of a condition adjusting mechanism during culture, adjusting the temperature in the culture dish by a heating pipe, controlling the humidity adjustment by a closed cylinder of the culture mechanism, and checking the culture dish by a seal cover on the culture mechanism; after the culture is finished, a material taking mechanism of the material taking dilution screening system is matched with an extraction cylinder to extract a sample, a plurality of test tubes are arranged on the material taking dilution screening system, the screening mechanism releases the test solution absorbed by the last test tube for a plurality of times, the operation is performed for a plurality of times to carry out gradient dilution circularly, and after the gradient dilution screening is finished, the test solution is dropped on a tail-end observation dish; observe the intraductal condition of examination and test the composition in the examination during this, if discover wherein certain test tube can't satisfy the requirement, screening mechanism will wholly get the material and dilute the horizontal rotation of screening system, avoid the test tube that has the problem, accomplish the screening culture.
2. The method for screening and culturing microorganisms for animal husbandry according to claim 1, wherein: the base is a mounting platform of the culture mechanism, the condition adjusting mechanism and the material taking, diluting and screening system; the base includes support, water tank 102, and water tank fixed mounting is on the support, and the inside top of water tank is provided with the filter screen, and the water tank side is provided with two water pipes, is provided with the humidification valve respectively on two water pipes and cultivates the valve.
3. The method for screening and culturing microorganisms for animal husbandry according to claim 1, wherein: the culture mechanism comprises a culture box, a closed cylinder and a culture dish; the rotary mounting has the sealed cowling on the incubator, and sealed cylinder fixed mounting is in the side of incubator, and sealed cylinder end fixing has the shutoff board, is provided with the hole of humidifying on the incubator, and incubator fixed mounting has the cultivation slide rail, and slidable mounting has the culture dish on the cultivation slide rail.
4. The method for screening and culturing microorganisms for animal husbandry according to claim 1, wherein: the condition adjusting mechanism comprises a humidifying fan and a heating pipe; the humidifying fan is fixedly arranged at a humidifying hole on the incubator, and the heating pipe is fixedly arranged at the bottom of the bracket and is arranged right below the culture mechanism; a humidifying core is arranged between the humidifying fan and the humidifying hole, the humidifying core is connected with a water pipe with a humidifying valve on the water tank, and the heating pipe is fixedly installed at the bottom of the support.
5. The method for screening and culturing microorganisms for animal husbandry according to claim 1, wherein: get material and dilute the screening system and include feeding agencies, screening mechanism, an extraction section of thick bamboo, feeding agencies fixed mounting is on the support, and screening mechanism sets up in feeding agencies's below, and an extraction section of thick bamboo fixed mounting is provided with test tube and observation ware on the screening mechanism.
6. The method for screening and culturing microorganisms for animal husbandry according to claim 5, wherein: the material taking mechanism comprises a material taking slide rail and a pen-shaped air cylinder; get material slide rail fixed mounting on the support, get material slide rail slidable mounting and have the slider, connecting plate fixed mounting is on the slider, and the tip of stroke-shaped cylinder passes through connecting plate fixed mounting on the slider.
7. The method for screening and culturing microorganisms for animal husbandry according to claim 5, wherein: the screening mechanism comprises an upper position cylinder, a lower position cylinder and a screening frame; the screening frame is rotatably installed on a stretching seat below the upper position air cylinder and the lower position air cylinder, the screening frame penetrates through the motor base and is fixedly installed with the transmission gear shaft, the upper position air cylinder and the lower position air cylinder are fixedly installed at the bottom of the connecting plate, the stretching seat is fixedly installed at the end portion of the upper position air cylinder and the lower position air cylinder, the transmission gear shaft is rotatably installed on the motor base, the gear end of the transmission gear shaft is meshed with the power gear to rotate, and the power gear is fixedly installed with the motor.
8. The method for screening and culturing microorganisms for animal husbandry according to claim 1, wherein: the extraction cylinder comprises a sliding frame and a suction pipe; the sliding frame is slidably mounted on the suction pipe, the piston lead screw is rotatably mounted on the sliding frame, the end part of the piston lead screw is fixedly provided with the piston, the piston lead screw is rotatably mounted on the sliding frame and the nut seat, the nut seat is fixedly mounted on the suction pipe, the piston penetrates through the nut seat and is rotatably mounted at the bottom of the piston lead screw, and the piston is arranged inside the suction pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110636641.6A CN113355225B (en) | 2021-06-08 | 2021-06-08 | Microorganism screening and culturing process for animal husbandry |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110636641.6A CN113355225B (en) | 2021-06-08 | 2021-06-08 | Microorganism screening and culturing process for animal husbandry |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113355225A CN113355225A (en) | 2021-09-07 |
| CN113355225B true CN113355225B (en) | 2022-03-04 |
Family
ID=77533141
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110636641.6A Active CN113355225B (en) | 2021-06-08 | 2021-06-08 | Microorganism screening and culturing process for animal husbandry |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113355225B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106893673A (en) * | 2017-04-11 | 2017-06-27 | 尹康康 | A kind of soil bacteria separates counting experimental provision |
| CN109971640A (en) * | 2019-04-13 | 2019-07-05 | 河南省中医院（河南中医药大学第二附属医院） | Clinical laboratory's bacterial incubator |
| CN209128174U (en) * | 2018-10-31 | 2019-07-19 | 泰州国安医疗用品有限公司 | A kind of industrial water purification and sterilization system |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017127727A1 (en) * | 2016-01-21 | 2017-07-27 | T2 Biosystems, Inc. | Rapid antimicrobial susceptibility testing using high-sensitivity direct detection methods |
-
2021
- 2021-06-08 CN CN202110636641.6A patent/CN113355225B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106893673A (en) * | 2017-04-11 | 2017-06-27 | 尹康康 | A kind of soil bacteria separates counting experimental provision |
| CN209128174U (en) * | 2018-10-31 | 2019-07-19 | 泰州国安医疗用品有限公司 | A kind of industrial water purification and sterilization system |
| CN109971640A (en) * | 2019-04-13 | 2019-07-05 | 河南省中医院（河南中医药大学第二附属医院） | Clinical laboratory's bacterial incubator |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113355225A (en) | 2021-09-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110951589A (en) | Microbial detection clean culture device and use method thereof | |
| CN113355225B (en) | Microorganism screening and culturing process for animal husbandry | |
| CN113755331B (en) | Cell incubator of simulation altitude environment | |
| CN217025972U (en) | Cultivate efficient constant temperature incubator | |
| CN115322899A (en) | Cell culture device for cell biology experiments | |
| CN2799572Y (en) | Automatic separation and culturing device for biological sample | |
| CN114058506B (en) | Efficient pig raising method and system for improving maturation rate of oocytes | |
| CN113188865B (en) | Before processing apparatus is used in excrement and urine sample test for intensive care unit | |
| CN108342315A (en) | A kind of experiment culture and detection germ culturing device | |
| CN219117439U (en) | Germ spore capturing analyzer | |
| CN112167059A (en) | Rhizoma bletillae tissue culture rooting culture device and method | |
| CN111607518A (en) | Automatic IVF culture and time difference analysis device and matched culture dish | |
| CN106318861A (en) | Automatic microorganism separate culture instrument system | |
| CN107402142B (en) | Aquatic animal excrement and urine collection system | |
| CN220034524U (en) | Biochemical incubator with monitoring function | |
| CN220887519U (en) | Environment DNA collection system in air | |
| CN216712096U (en) | Can automated inspection and adjust bacterial culture case of humiture | |
| CN220132228U (en) | Be used for cell biopsy culture apparatus | |
| CN117143697B (en) | Preparation equipment, preparation method and application of composite microbial inoculum for treating kitchen waste gas | |
| CN210796482U (en) | A incubator structure for detecting bacterial colony large intestine | |
| CN201560183U (en) | Biological sample single-culture-medium separation culture apparatus | |
| CN217052262U (en) | Paper sheet carrier fixed bed culture online digestive cell passage bioreactor | |
| CN214571971U (en) | Biocontrol actinomycete liquid storage device | |
| CN216946959U (en) | Anti-pollution formula bacteria culture device of clinical laboratory | |
| CN220224190U (en) | Full-automatic cell sampling culture device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A screening and culture process of microorganisms for animal husbandry Effective date of registration: 20220630 Granted publication date: 20220304 Pledgee: Shandong Shanghe Rural Commercial Bank Co.,Ltd. Pledgor: Shandong tianrunhe Bioengineering Co.,Ltd. Registration number: Y2022980009543 |