CN220887519U - Environment DNA collection system in air - Google Patents
Environment DNA collection system in air Download PDFInfo
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- CN220887519U CN220887519U CN202322434290.8U CN202322434290U CN220887519U CN 220887519 U CN220887519 U CN 220887519U CN 202322434290 U CN202322434290 U CN 202322434290U CN 220887519 U CN220887519 U CN 220887519U
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- air
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- way valve
- filter membrane
- sampling tube
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- 238000005070 sampling Methods 0.000 claims abstract description 87
- 230000007613 environmental effect Effects 0.000 claims abstract description 42
- 238000000605 extraction Methods 0.000 claims abstract description 21
- 239000012528 membrane Substances 0.000 claims description 41
- 238000005086 pumping Methods 0.000 claims description 3
- 238000003306 harvesting Methods 0.000 claims 3
- 239000002245 particle Substances 0.000 description 10
- 239000000126 substance Substances 0.000 description 10
- 241001465754 Metazoa Species 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
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Abstract
The utility model relates to an environmental DNA acquisition device in air, which comprises a frame, a sampling tube loading assembly, a multi-way valve and an air extraction assembly 7; the rack is provided with an air extraction assembly 7 for providing a bearing platform; the air extraction assembly is connected with the sampling tube loading assembly through a multi-way valve and is used for providing negative pressure for the sampling tube loading assembly; the air inlet of the sampling tube loading assembly faces the surrounding environment for collecting air under negative pressure and adhering to the ambient DNA. The device can realize continuous collection of the environmental DNA in the air, solves the problem of singleness of single-tube sampling, and improves the efficiency of sampling the environmental DNA in the air.
Description
Technical Field
The utility model belongs to the technical field of collection of environmental DNA in air, and relates to an environmental DNA collection device in air.
Background
With the progress of science and technology, more means are available for monitoring the ecological environment, such as the collection of environmental DNA in the air, the transmission and distribution of pathogens, the composition of plant pollen, animals moving in a region, and the like. All of the above work requires the collection of environmental DNA in the air, followed by nucleic acid extraction, sequencing or other molecular biological analysis of the collected material for its composition, such as microorganisms (including pathogens), plants, animals, and the like. Meanwhile, to clarify the differences in different time periods and the repeatability of the experiment, it is often necessary to collect a plurality of samples in succession.
The existing environmental DNA collection device in the air is mainly used for single-tube collection, samples cannot be collected rapidly and continuously by single-tube collection, and the collection of a plurality of samples can be completed only by more collectors and manual duty.
Therefore, the environmental DNA collection device in the prior art has the technical problems that a plurality of samples cannot be collected rapidly and continuously, and the air collection efficiency is low.
Based on the above problems, the present utility model provides an environmental DNA collection device in air.
Disclosure of utility model
In order to solve the technical problems, the utility model provides the air environment DNA acquisition device, which adopts a plurality of sampling pipes to realize continuous sampling of air, solves the problem of singleness of air single-pipe sampling, improves the efficiency of air sampling, and ensures that the accuracy of the collected air is higher.
In order to achieve the above purpose, the technical scheme adopted by the utility model is as follows:
An environment DNA collection system in air, its characterized in that: comprises a frame, a sampling tube loading assembly, a multi-way valve and an air extraction assembly;
The rack is provided with an air extraction assembly for providing a bearing platform;
The air extraction assembly is connected with the sampling tube loading assembly through a multi-way valve and is used for providing negative pressure for the sampling tube loading assembly;
The air inlet of the sampling tube loading assembly faces the surrounding environment for collecting air under negative pressure and adhering to the environmental DNA.
Further, the sampling tube loading assembly comprises a plurality of sampling tubes and a loading plate, wherein the sampling tubes are arranged on the loading plate at intervals along the horizontal direction and used for adhering the environmental DNA in the air passing through the sampling tubes; the multi-way valve is respectively connected with each sampling tube.
Further, each sampling tube comprises a tube body and a filter membrane, wherein an air inlet is formed in the top of the tube body, an air outlet is formed in the bottom of the tube body, the air outlet is communicated with the multi-way valve, and the filter membrane is arranged in the tube body along the horizontal direction and is used for sticking and covering environmental DNA in the air passing through the filter membrane.
Further, each sampling tube further comprises a filter membrane support, the filter membrane supports are horizontally arranged inside the tube body, a plurality of air inlets are formed in the filter membrane supports, and the filter membranes are paved above the filter membrane supports.
Further, the air pump is selected as the air pumping component.
Further, the multi-way valve is communicated with the air pump through a three-way valve.
Due to the adoption of the technical scheme, the utility model has the following advantages:
The air extraction component is connected with the sampling tube loading component through the multi-way valve and is used for providing negative pressure for the sampling tube loading component, and the air inlet of the sampling tube loading component faces the surrounding environment and is used for collecting air under the negative pressure and pasting and covering environmental DNA. The multi-way valve and the sampling tube loading assembly are sequentially connected from left to right, when the sampling is needed, the air extraction assembly is started, the multi-way valve is opened, the air extraction assembly sucks air into the sampling tube loading assembly, the air is sucked into the sampling tube loading assembly, and the air is collected under negative pressure and is adhered to the environmental DNA. Compared with the prior art, the device is provided with a sampling tube loading assembly, and a plurality of sampling tubes are arranged on the sampling tube loading assembly; when the sampling device is used, the sampling pipes are connected with the air pump through the multi-way valve, so that the continuous collection of the environmental DNA in the air can be realized, the singleness of single-pipe sampling is solved, and the efficiency of sampling the environmental DNA in the air is improved.
Drawings
FIG. 1 is a schematic diagram of an environmental DNA collection device in air according to the present utility model.
The reference numerals are as follows: 1-frame, 2-sampling pipe loading assembly, 22-sampling pipe, 221-body, 222-filter membrane bracket, 223-filter membrane, 3-air inlet, 4-air outlet, 5-multiport valve, 6-three-way valve, 7-air pump, 8-control switch, 9-power, 10-controller.
Detailed Description
The preferred embodiments of the present utility model will be described in detail below with reference to the attached drawings, so that the objects, features and advantages of the present utility model will be more clearly understood. It should be understood that the embodiments shown in the drawings are not intended to limit the scope of the utility model, but rather are merely illustrative of the true spirit of the utility model.
The utility model relates to an environment DNA collecting device in air, which comprises a frame, wherein an air extraction assembly is arranged on the frame, the air extraction assembly is connected with a sampling tube loading assembly through a multi-way valve, the sampling tube loading assembly comprises a plurality of sampling tubes and a loading plate, each sampling tube comprises a tube body, a filter membrane and a filter membrane support, the top of the tube body is provided with an air inlet, the bottom of the tube body is provided with an air outlet, the air outlet is communicated with the multi-way valve, the filter membrane support is arranged in the tube body, a plurality of air inlets are arranged on the filter membrane support, the filter membrane is paved above the filter membrane support, and the air extraction assembly is connected with the plurality of sampling tubes through the multi-way valve to continuously collect the environment DNA in the air. The device has set up a plurality of sampling pipe, and during the use, is connected a plurality of sampling pipe through the multiport valve with bleed subassembly, can realize the environment DNA in the continuous collection air, has solved the singleness of single tube sampling, has improved the efficiency of environment DNA sampling in the air.
As shown in fig. 1. The utility model provides an environmental DNA acquisition device in air, which comprises a frame 1, a sampling tube loading assembly 2, a multi-way valve 5 and an air extraction assembly; the frame 1 is provided with an air extraction component for providing a bearing platform; the air extraction component is connected with the sampling tube loading component 2 through a multi-way valve 5 and is used for providing negative pressure for the sampling tube loading component 2; the air inlet 3 of the sample tube loading assembly 2 is facing the surrounding environment for collecting air under negative pressure and for sticking to the surrounding DNA.
Specifically, the sampling tube loading assembly 2 comprises a sampling tube 22, a loading plate 11, and a plurality of sampling tubes 22 are arranged on the loading plate 11 at intervals along the horizontal direction for sticking the environmental DNA in the air passing through the sampling tube 22; a multi-way valve 5 is connected to each sampling tube 22.
Specifically, the sampling tube 22 includes a tube body 221 and a filter membrane 223, and a filter membrane of 0.2 or 0.45um is generally selected for collection; the top of the tube body 221 is provided with an air inlet 3, the bottom of the tube body is provided with an air outlet 4, the air outlet 4 is communicated with a multi-way valve 5, the multi-way valve 5 adopts multiple inlets and multiple outlets, and multiple inlets are correspondingly connected with the air outlets 4 on the tube body 221 on the multiple sampling tubes 22 one by one; the filter 223 is disposed in the tube 221 in a horizontal direction for binding the environmental DNA in the air passing through the filter 223. The filter membrane has the function of keeping large-particle substances such as environmental DNA in the air on the filter membrane, so that the sampling of the large-particle substances such as the environmental DNA is achieved.
Specifically, each sampling tube 22 further includes a filter membrane support 222, the filter membrane support 222 is disposed inside the tube body 221 along a horizontal direction, a plurality of air intake holes are formed in the filter membrane support 222, and the filter membrane 223 is laid over the filter membrane support 222. The filter membrane bracket has the function of supporting and fixing the filter membrane in the tube body; the continuous collection of the environmental DNA in the air is realized. The multi-way valve and the sampling tube are sequentially connected from left to right, when the sampling tube is required to be collected, the air extraction assembly is started, the first passage in the multi-way valve 5 is opened, the rest passage is closed, the air extraction assembly sucks air into the first sampling tube, the air is sucked into the sampling tube, large-particle substances such as environmental DNA in the air are left on the filter membrane through the filter membrane, sampling of the large-particle substances such as the environmental DNA is completed, and the small-particle substances are discharged. After the first tube collection is completed, the first passage is closed, the second passage is opened, the second tube collection is performed, and so on according to the connection sequence of the multi-way valve and the sampling tube until the sampling tube on the loading plate 11 is completed.
Preferably, the air pump 7 is selected as the air pumping component; the multi-way valve 5 is communicated with the air pump 7 through the three-way valve 6. The air pump 7 is connected with an inlet A of the three-way valve 6, and the multi-way valve 5 is connected with an inlet B of the three-way valve 6; the air is sucked into the sampling tube along the suction path of the air pump 7, the inlet A, the inlet B and the multi-way valve 5, and the air is sucked into the sampling tube and passes through the filter membrane to leave large-particle substances such as environmental DNA and the like in the air on the filter membrane for detection. After the collection of large-particle substances such as environmental DNA in the air is completed, the controller 10 is started to change the valve core of the three-way valve 6, the inlet B is closed, the outlet C is opened, and the small-particle substances are discharged along the outlet C.
Preferably, because air collection devices generally require outdoor or field collection and require manual duty, more intelligent collection devices are required to avoid manual duty. In order to improve the efficiency of DNA sampling in the air environment, the accuracy of the collected DNA in the air environment is higher; the camera is arranged on the acquisition device, so that the change of the acquisition environment can be observed through a remote App at any time; the collection time and the air flow of each sample can be set remotely; and acquiring environmental parameters such as temperature, humidity and the like of the sampling environment in real time.
The frame 1 is internally provided with a power supply 9 and a controller 10, the outside of the frame is provided with a control switch 8, and the controller 10 is electrically connected with the power supply 9, the multi-way valve 5, the three-way valve 6 and the air pump 7.
As shown in fig. 1. When the device for collecting the environmental DNA in the air is used, the control switch is turned on, the controller controls the air pump to work, the controller controls the multi-way valve to open the first passage and close other passages, the suction path of the air pump sucks the air into the first sampling tube along the inlet A and the inlet B of the three-way valve and the first passage of the multi-way valve, the air is sucked into the sampling tube, and large particulate matters such as the environmental DNA in the air are left on the filter membrane through the filter membrane for detection. After the collection of large-particle substances such as environmental DNA in the air is completed, a controller is started to enable the valve core of the three-way valve to change direction, the inlet B is closed, the outlet C is opened, and small-particle substances are discharged along the outlet C. After the first tube collection is completed, the first passage is closed, the second passage is opened, the second tube collection is performed, and so on according to the connection sequence of the multi-way valve and the sampling tube until the sampling tube on the loading plate 11 is completed. Compared with the prior art, the device is provided with a plurality of sampling pipes, can continuously collect the environmental DNA in the air, solves the problem of singleness of single-pipe sampling, and improves the efficiency of environmental DNA sampling in the air.
Claims (6)
1. An environment DNA collection system in air, its characterized in that: comprises a frame (1), a sampling tube loading assembly (2), a multi-way valve (5) and an air extraction assembly;
an air extraction assembly is arranged on the frame (1) and used for providing a bearing platform;
The air extraction assembly is connected with the sampling tube loading assembly (2) through a multi-way valve (5) and is used for providing negative pressure for the sampling tube loading assembly (2);
The air inlet (3) of the sampling tube loading assembly (2) faces the surrounding environment and is used for collecting air under negative pressure and pasting and covering the environmental DNA.
2. The in-air environmental DNA harvesting device of claim 1, wherein: the sampling tube loading assembly (2) comprises a plurality of sampling tubes (22) and a loading plate (11), wherein the sampling tubes (22) are arranged on the loading plate (11) at intervals along the horizontal direction and are used for adhering and covering the environmental DNA in the air passing through the sampling tubes (22); the multi-way valve (5) is respectively connected with each sampling tube (22).
3. The in-air environmental DNA harvesting device of claim 2, wherein: each sampling tube (22) comprises a tube body (221) and a filter membrane (223), an air inlet (3) is formed in the top of the tube body (221), an air outlet (4) is formed in the bottom of the tube body, the air outlet (4) is communicated with the multi-way valve (5), and the filter membrane (223) is arranged in the tube body (221) along the horizontal direction and is used for adhering and covering environmental DNA in air passing through the filter membrane (223).
4. The in-air environmental DNA harvesting device of claim 3, wherein: each sampling tube (22) further comprises a filter membrane support (222), the filter membrane support (222) is horizontally arranged inside the tube body (221), a plurality of air inlets are formed in the filter membrane support (222), and the filter membrane (223) is paved above the filter membrane support (222).
5. An airborne environmental DNA collection system according to any one of claims 1-4, wherein: the air pumping component is an air pump (7).
6. An airborne environmental DNA collection system in accordance with claim 5, wherein: the multi-way valve (5) is communicated with the air pump (7) through a three-way valve (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322434290.8U CN220887519U (en) | 2023-09-07 | 2023-09-07 | Environment DNA collection system in air |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322434290.8U CN220887519U (en) | 2023-09-07 | 2023-09-07 | Environment DNA collection system in air |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220887519U true CN220887519U (en) | 2024-05-03 |
Family
ID=90870252
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322434290.8U Active CN220887519U (en) | 2023-09-07 | 2023-09-07 | Environment DNA collection system in air |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220887519U (en) |
-
2023
- 2023-09-07 CN CN202322434290.8U patent/CN220887519U/en active Active
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