CN114058506B - Efficient pig raising method and system for improving maturation rate of oocytes - Google Patents
Efficient pig raising method and system for improving maturation rate of oocytes Download PDFInfo
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- CN114058506B CN114058506B CN202111409891.2A CN202111409891A CN114058506B CN 114058506 B CN114058506 B CN 114058506B CN 202111409891 A CN202111409891 A CN 202111409891A CN 114058506 B CN114058506 B CN 114058506B
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- 210000000287 oocyte Anatomy 0.000 title claims abstract description 68
- 230000035800 maturation Effects 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 19
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 118
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 59
- 239000001569 carbon dioxide Substances 0.000 claims description 59
- 239000007788 liquid Substances 0.000 claims description 40
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 27
- 239000012530 fluid Substances 0.000 claims description 25
- 239000002699 waste material Substances 0.000 claims description 20
- 238000001802 infusion Methods 0.000 claims description 19
- 230000001502 supplementing effect Effects 0.000 claims description 12
- 238000012545 processing Methods 0.000 claims description 11
- 241000282887 Suidae Species 0.000 claims description 9
- 238000007599 discharging Methods 0.000 claims description 9
- 238000010438 heat treatment Methods 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 8
- 230000000694 effects Effects 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 5
- 230000001681 protective effect Effects 0.000 claims description 4
- 238000001514 detection method Methods 0.000 claims description 3
- 238000004113 cell culture Methods 0.000 abstract description 2
- 230000008859 change Effects 0.000 abstract description 2
- 238000002637 fluid replacement therapy Methods 0.000 description 8
- 239000012531 culture fluid Substances 0.000 description 7
- 238000000338 in vitro Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 3
- 238000005485 electric heating Methods 0.000 description 3
- 230000002457 bidirectional effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 210000001503 joint Anatomy 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000021121 meiosis Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
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- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M23/10—Petri dish
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0608—Germ cells
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Abstract
The invention relates to the field of cell culture, and particularly discloses a high-efficiency pig raising method and a high-efficiency pig raising system for improving the maturation rate of oocytes. The invention is convenient for automatically completing the replacement of the oocyte culture solution, reduces the labor capacity of researchers, does not change the culture environment of the oocyte, is very convenient and fast to observe and record the culture condition of the oocyte, and is convenient to popularize and use.
Description
Technical Field
The invention relates to the field of cell culture, and particularly discloses a high-efficiency pig raising method and a high-efficiency pig raising system for improving the maturation rate of oocytes.
Background
In vitro culture (IVM) of mammalian oocytes is an important component of embryo engineering, which means that the immature oocytes taken out from follicles are subjected to in vitro maturation culture, developed to the midperiod of meiosis second, can undergo in vitro fertilization and split into embryos, pigs are taken as a common model organism, the in vitro maturation of the oocytes is studied from the end of the 80 th century, and through more years of development, although great progress has been made, the in vitro maturation of porcine oocytes still has a great gap between maturation quality and development potential compared with in vivo.
In the prior art, in case that a culture solution needs to be replaced and the condition of oocyte culture is observed in the oocyte culture process, researchers are required to take out the oocyte from the inside of the incubator for manual replacement, the process is complicated, the culture environment of the oocyte is changed, the culture effect is affected, and the use is inconvenient, so that the efficient pig raising method and the culture system for improving the maturation rate of the oocyte are provided for solving the problems.
Disclosure of Invention
In view of the above, the present invention aims to provide a high-efficiency pig raising method and a high-efficiency pig raising system for improving the maturation rate of oocytes.
In order to achieve the above purpose, the present invention provides the following technical solutions: the utility model provides a improve high-efficient culture system who raises pigs of maturation rate of oocyte, includes the carbon dioxide incubator, the inside of carbon dioxide incubator is provided with the sliding plate, the inside of sliding plate is provided with a set of culture dish, the inside and the outside of carbon dioxide incubator are provided with the fluid infusion subassembly, the inside of carbon dioxide incubator is provided with the flowing back subassembly, control panel is installed in the outside of carbon dioxide incubator, the time-recorder is installed in the outside of carbon dioxide incubator.
Further, the culture dish includes the disk body, filter disc, pipe and solenoid valve, filter disc fixed connection is to the inside of the disk body, the pipe runs through and fixed connection is to the inside of the disk body, the solenoid valve is installed to the middle part of pipe, the bottom fixedly connected with of pipe with the clamping lid of its intercommunication, the clamping lid inlays to the inside of sliding plate, the filter disc goes up the filter pore inner diameter and is 0.04mm.
Further, the flowing back subassembly includes waste liquid case, a set of fluid-discharge tube, violently pipe, first hose and a set of junction box, waste liquid case fixed connection is to the interior bottom wall of carbon dioxide incubator, a set of junction box respectively the joint to the inside of a set of joint lid, a set of the fluid-discharge tube runs through respectively and fixed connection to the inside of a set of junction box, a set of the fluid-discharge tube all runs through and fixed connection to the inside of violently pipe, first hose runs through and fixed connection to the inside of waste liquid case, a set of the inside of junction box all inlays and is equipped with the sealing washer.
Further, a set of middle part fixedly connected with diaphragm of fluid-discharge tube, diaphragm sliding connection is to the inside of carbon dioxide incubator, the outside fixedly connected with two springs of diaphragm, two the equal fixedly connected with of spring is to the inside of carbon dioxide incubator.
Further, the fluid replacement subassembly includes storage box, water pump, second hose, shunt tubes, a set of fluid replacement pipe and a set of metering valve, storage box fixed connection is to the outside of carbon dioxide incubator, the water pump is installed to the outside of carbon dioxide incubator, the inside fixedly connected with fixed pipe of storage box, the input and the fixed pipe fixed connection of water pump, the output and the second hose fixed connection of water pump, the second hose runs through and extends to the inside of carbon dioxide incubator, the second hose runs through and fixed connection is to the inside of shunt tubes, a set of fluid replacement pipe all runs through and fixed connection is to the inside of shunt tubes, a set of metering valve installs respectively to the middle part of a set of fluid replacement pipe.
Further, two heating wires are installed in the storage box, and a temperature sensor is installed on the outer side of the storage box.
Further, two electric push rods are fixedly connected to the inside of the carbon dioxide incubator, and the bottom ends of the two electric push rods are fixedly connected with the shunt tubes.
Further, a group of the bottom ends of the fluid infusion pipes penetrate through and are fixedly connected with a protection box, and two small electron microscopes are arranged on the outer sides of the protection boxes.
Further, a central processor is arranged in the control panel, the output end of the central processor is respectively in signal connection with the input ends of the metering valve, the electromagnetic valve, the water pump, the heating wire, the carbon dioxide incubator and the electric putter, and the central processor is respectively in bidirectional signal connection with the miniature electron microscope, the timer and the temperature sensor.
The invention also provides a method for raising pigs efficiently by improving the maturation rate of oocytes, which comprises the following specific steps:
s1, researchers can respectively place oocytes prepared in advance in a group of dish bodies, then insert a sliding plate into the carbon dioxide incubator, and drive the carbon dioxide incubator to operate by using a control panel.
S2, preheating the culture solution in the storage box by using two electric heating wires, after the temperature reaches the temperature, enabling the temperature sensor to receive detection data, then operating by using a water pump, leading the culture solution out of the storage box, then respectively discharging the culture solution into the inside of a group of dish bodies by using a group of fluid infusion pipes, opening a group of metering valves in the fluid infusion process, facilitating the quantitative discharge of the culture solution, then enabling the culture solution to be in contact with oocytes, and then stopping the operation of the water pump and the group of metering valves.
S3, when the fluid infusion is completed, the two electric push rods are used for operating to drive the small electron microscope to operate to a preset position, researchers can use the timer to perform timing of 4 hours, 8 hours and 12 hours, observe and record the condition of the oocyte at intervals, the condition of the oocyte can be observed visually by using the small electron microscope, a carbon dioxide incubator is not required to be opened, and the culture environment of the oocyte is ensured.
And S4, after 12 hours, the timer sends data to the central processing unit, then the electromagnetic valve is opened, so that the culture solution flows into the waste liquid box along a group of liquid discharge pipes, the culture solution level gradually descends, and the oocytes are conveniently blocked by the arrangement of the filter discs.
S5, the water pump and the metering valve are used for operating again, the culture solution is supplemented into the dish body, the culture solution replacement effect is achieved, the whole process is not required to be manually replaced by a worker, the oocyte is always located in the carbon dioxide incubator, a good culture environment is ensured, and the oocyte can be taken out after continuous culture for 16 hours in the carbon dioxide incubator.
The working principle and the beneficial effects of the scheme are as follows:
(1) This scheme is when need carry out the fluid infusion work, available water pump operation, thereby with the inside culture solution of storage tank by the fixed pipe, the inside of the leading-in shunt tubes of second hose, afterwards the inside of culture solution reposition of redundant personnel to a set of fluid infusion pipe, utilize a set of metering valve operation, be convenient for make quantitative culture solution discharge respectively, make the downward whereabouts of culture solution, thereby get into the internal portion of corresponding dish, contact with oocyte, utilize the connecting cover to dock with the card lid, the setting of sealing washer can effectively reduce the culture solution appearance and take place, when carrying out the flowing back work, utilize the solenoid valve to open, waste liquid will flow downwards by the pipe afterwards, collect the inside of violently managing through a set of fluid-discharge tube, finally all by the inside of leading-in waste liquid case of first hose, be convenient for collect the waste liquid, discharge to the waste liquid and new liquid's replenishment have been accomplished to whole in-process, and is convenient and fast.
(2) This scheme is 0.04mm according to the filter screen inner diameter on the filter disc to play the stopping effect to the oocyte when being convenient for the culture solution to flow, reduce the oocyte and follow the phenomenon that the culture solution was led into the internal portion of ware and take place, utilize the cooperation of pipe and solenoid valve to use, be convenient for control the discharge of culture solution, utilize the setting of card lid, be convenient for place the internal portion at the sliding plate with the ware body, be convenient for the researcher to take to place to single ware body.
(3) The small-size electron microscope's in this scheme utilization protection box outside setting is convenient for the researcher need not to take out the ware body, can observe the oocyte and cultivate the condition, is convenient for observe, record in real time.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention. The objects and other advantages of the invention may be realized and obtained by means of the instrumentalities and combinations particularly pointed out in the specification.
Drawings
FIG. 1 is a schematic diagram showing the structure of a high-efficiency pig raising culture system for improving the maturation rate of oocytes;
FIG. 2 is a schematic diagram showing a schematic view in front cross-section of the structure of a culture system for efficient pig raising to increase the maturation rate of oocytes according to the present invention;
FIG. 3 is an enlarged schematic view of A in FIG. 2, showing a structure of a system for efficient pig raising to increase the maturation rate of oocytes according to the present invention;
FIG. 4 is an exploded view showing the structure of a culture dish and a connecting cover of the culture system for efficiently raising pigs, which can improve the maturation rate of oocytes;
FIG. 5 is an enlarged schematic view of B in FIG. 2, showing a structure of a system for efficient pig raising to increase the maturation rate of oocytes according to the present invention;
FIG. 6 is a schematic diagram of a system flow of a method for efficient pig raising to increase oocyte maturation rate according to the present invention.
The figures are marked as follows: 1. a carbon dioxide incubator; 2. a sliding plate; 3. a culture dish; 301. a dish body; 302. a filter disc; 303. a conduit; 304. an electromagnetic valve; 4. a fluid replacement assembly; 401. a storage box; 402. a water pump; 403. a second hose; 404. a shunt; 405. a fluid supplementing pipe; 406. a metering valve; 5. a liquid discharge assembly; 501. a waste liquid tank; 502. a liquid discharge pipe; 503. a transverse tube; 504. a first hose; 505. a connection cover; 6. a control panel; 7. a clamping cover; 8. a cross plate; 9. a spring; 10. heating wires; 11. a temperature sensor; 12. an electric push rod; 13. a protective case; 14. a small electron microscope; 15. a central processing unit; 16. a timer.
Detailed Description
The following is a further detailed description of the embodiments:
referring to fig. 1 to 6, the culture system for raising pigs efficiently for improving the maturation rate of oocytes in the invention comprises a carbon dioxide incubator 1, wherein a sliding plate 2 is arranged in the carbon dioxide incubator 1, a group of culture dishes 3 are arranged in the sliding plate 2, a fluid supplementing component 4 is arranged in and outside the carbon dioxide incubator 1, a fluid draining component 5 is arranged in the carbon dioxide incubator 1, a control panel 6 is arranged outside the carbon dioxide incubator 1, and a timer 16 is arranged outside the carbon dioxide incubator 1.
According to the invention, a researcher can correspondingly place oocytes in a group of culture dishes 3, then insert a sliding plate 2 for supporting the group of culture dishes 3 into the carbon dioxide incubator 1, then supplement culture solution to the culture dishes 3 by using a fluid supplementing assembly 4, control electric elements in the whole device conveniently by using the arrangement of a control panel 6, time counting of the culture time of the oocytes is conveniently carried out by using the arrangement of a timer 16, when the culture time reaches a preset value, the fluid draining assembly 5 is used for running, the culture solution in the culture dishes 3 is drained, and meanwhile, the fluid supplementing assembly 4 is used for supplementing the culture solution again, so that the culture solution replacement effect is achieved.
Please refer to fig. 1-4, wherein: the culture dish 3 includes the disk body 301, filter disc 302, pipe 303 and solenoid valve 304, and filter disc 302 fixed connection is to the inside of disk body 301, and pipe 303 runs through and fixed connection is to the inside of disk body 301, and solenoid valve 304 installs the middle part of pipe 303, and the bottom fixedly connected with of pipe 303 has the clamping lid 7 with its intercommunication, and clamping lid 7 inlays to the inside of sliding plate 2, and the filter pore inner diameter is 0.04mm on the filter disc 302.
According to the invention, according to the inner diameter of the filter hole on the filter disc 302 of 0.04mm, the flow of the culture solution is facilitated, the blocking effect on the oocyte is realized, the phenomenon that the oocyte follows the culture solution to be led into the interior of the dish body 301 is reduced, the discharge of the culture solution is conveniently controlled by utilizing the cooperation of the guide tube 303 and the electromagnetic valve 304, the dish body 301 is conveniently placed in the interior of the sliding plate 2 by utilizing the arrangement of the clamping cover 7, and the study staff can conveniently take and place the single dish body 301.
Please refer to fig. 1-3, wherein: the liquid discharging assembly 5 comprises a waste liquid tank 501, a group of liquid discharging pipes 502, a transverse pipe 503, a first hose 504 and a group of connecting covers 505, wherein the waste liquid tank 501 is fixedly connected to the inner bottom wall of the carbon dioxide incubator 1, the group of connecting covers 505 are respectively clamped into the group of clamping covers 7, the group of liquid discharging pipes 502 respectively penetrate through and are fixedly connected to the group of connecting covers 505, the group of liquid discharging pipes 502 respectively penetrate through and are fixedly connected to the inner parts of the transverse pipe 503, the first hose 504 penetrates through and is fixedly connected to the inner parts of the waste liquid tank 501, and sealing rings are embedded into the group of connecting covers 505.
In the invention, the connection cover 505 is used for butt joint with the clamping cover 7, the sealing ring can effectively reduce the leakage phenomenon of the culture solution, when the liquid discharging work is carried out, the electromagnetic valve 304 is used for opening, then the waste solution flows downwards through the guide pipe 303, then is collected into the transverse pipe 503 through the group of liquid discharging pipes 502, and finally, the waste solution is completely guided into the waste solution tank 501 through the first hose 504, so that the waste solution can be conveniently collected.
Referring to fig. 1 to 5, a cross plate 8 is fixedly connected to the middle of a group of liquid discharge tubes 502, the cross plate 8 is slidably connected to the inside of the carbon dioxide incubator 1, two springs 9 are fixedly connected to the outside of the cross plate 8, and the two springs 9 are fixedly connected to the inside of the carbon dioxide incubator 1.
In the present invention, when a user needs to connect the sliding plate 2 to a set of culture dishes 3 for taking, the transverse plate 8 can be pressed in advance to compress a set of springs 9, at this time, a set of connecting covers 505 will be separated from a set of clamping covers 7, then the sliding plate 2 is convenient for taking, and after the placement is completed, the elastic force of the set of springs 9 is utilized to influence the set of connecting covers 505 to be connected with a set of clamping covers 7 again.
Referring to fig. 1 to 3, the fluid infusion assembly 4 includes a storage tank 401, a water pump 402, a second hose 403, a split-tube 404, a set of fluid infusion tubes 405 and a set of metering valves 406, wherein the storage tank 401 is fixedly connected to the outer side of the carbon dioxide incubator 1, the water pump 402 is mounted to the outer side of the carbon dioxide incubator 1, the interior of the storage tank 401 is fixedly connected with a fixed tube, an input end of the water pump 402 is fixedly connected with the fixed tube, an output end of the water pump 402 is fixedly connected with the second hose 403, the second hose 403 penetrates and extends into the interior of the carbon dioxide incubator 1, the second hose 403 penetrates and is fixedly connected to the interior of the split-tube 404, the set of fluid infusion tubes 405 penetrates and is fixedly connected to the interior of the split-tube 404, and the set of metering valves 406 are respectively mounted to the middle parts of the set of fluid infusion tubes 405.
In the invention, when the fluid replacement is needed, the water pump 402 is used to guide the culture fluid in the storage tank 401 into the shunt tube 404 through the fixed tube and the second hose 403, then the culture fluid is shunted into the fluid replacement tube 405, and the metering valve 406 is used to drain the quantitative culture fluid respectively, so that the culture fluid drops downwards to enter the corresponding dish 301 to contact with the oocyte.
Referring to fig. 1 to 2, two heating wires 10 are installed inside a storage box 401, and a temperature sensor 11 is installed outside the storage box 401.
In the invention, the arrangement of the two heating wires 10 is utilized to facilitate the preheating of the culture solution, and the arrangement of the temperature sensor 11 is utilized to facilitate the real-time detection of the temperature change of the culture solution.
Referring to fig. 1-2, two electric push rods 12 are fixedly connected to the inside of the carbon dioxide incubator 1, and bottom ends of the two electric push rods 12 are fixedly connected to the shunt tube 404.
In the invention, when the fluid infusion is performed, the two electric push rods 12 can be used to push the shunt tube 404 downwards, so that the distance between the bottom end of the fluid infusion tube 405 and the dish body 301 is reduced, the splashing phenomenon of the culture fluid is reduced, and when the fluid infusion is completed, the two electric push rods 12 can be operated to the original position.
Referring to fig. 1 to 3, the bottom ends of a set of fluid infusion tubes 405 penetrate and are fixedly connected with a protection box 13, and two small electron microscopes 14 are respectively mounted on the outer sides of the protection box 13.
In the invention, by using the arrangement of the small electron microscope 14 outside the protective box 13, researchers can observe the oocyte culturing condition without taking out the dish body 301, and real-time observation and recording are facilitated.
Referring to fig. 1 to 6, a central processing unit 15 is disposed in the control panel 6, an output end of the central processing unit 15 is respectively connected with a metering valve 406, an electromagnetic valve 304, a water pump 402, an electric heating wire 10, the carbon dioxide incubator 1 and an input end of an electric push rod 12, and the central processing unit 15 is respectively connected with a small electron microscope 14, a timer 16 and a temperature sensor 11 in a bidirectional signal manner.
In the invention, the central processing unit 15 is used for conveniently controlling the electric elements in the whole device, and the automatic completion of partial functions is realized more conveniently.
The invention also provides a method for raising pigs efficiently by improving the maturation rate of oocytes, which comprises the following specific steps:
s1, researchers can place pre-prepared oocytes in a group of dish bodies 301 respectively, then insert the sliding plate 2 into the carbon dioxide incubator 1, and drive the carbon dioxide incubator 1 to operate by using the control panel 6.
S2, preheating the culture solution in the storage tank 401 by using the two electric heating wires 10, after the temperature reaches 38 ℃, receiving detection data by the temperature sensor 11, then operating by using the water pump 402, leading the culture solution out of the storage tank 401, then respectively discharging the culture solution into the dish body 301 by using the liquid supplementing pipe 405, and opening the metering valve 406 in the liquid supplementing process so as to ensure quantitative discharge of the culture solution, contacting the oocyte by using the culture solution, and stopping the operation of the water pump 402 and the metering valve 406.
S3, when the fluid infusion is completed, the two electric push rods 12 are used for operating to drive the small electron microscope 14 to operate to a preset position, researchers can use the timer 16 to perform timing of 4 hours, 8 hours and 12 hours, observe and record the condition of the oocyte at intervals, the condition of the oocyte can be intuitively observed by using the small electron microscope 14, the carbon dioxide incubator 1 is not required to be opened, and the culture environment of the oocyte is ensured.
S4, after 12 hours, the timer 16 sends data to the central processing unit 15, then the electromagnetic valve 304 is opened, so that the culture solution flows into the waste liquid tank 501 along the group of liquid discharge pipes 502, the culture solution level gradually drops, and the oocytes are conveniently blocked by the arrangement of the filter discs 302.
S5, the water pump 402 and the metering valve 406 are used for operating again, the culture fluid is supplemented to the inside of the dish body 301, the culture fluid replacement effect is achieved, the whole process is not required to be manually replaced by staff, meanwhile, oocytes are always located in the carbon dioxide incubator 1, a good culture environment is ensured, and then the oocytes can be taken out after being continuously cultured in the carbon dioxide incubator 1 for 16 hours.
It should be noted that, each device in this application is common market equipment, can select according to the demand when specifically using, and the circuit connection relation of each device all belongs to simple series connection, parallel connection circuit, does not have innovation point at this circuit connection, and the realization that the technical staff can be comparatively easy belongs to prior art, and is not repeated.
The foregoing is merely exemplary embodiments of the present invention, and specific structures and features that are well known in the art are not described in detail herein. It should be noted that modifications and improvements can be made by those skilled in the art without departing from the structure of the present invention, and these should also be considered as the scope of the present invention, which does not affect the effect of the implementation of the present invention and the practical applicability of the present invention.
Claims (6)
1. The utility model provides an improve high-efficient culture system who raises pigs of maturation rate of oocyte, includes carbon dioxide incubator (1) and control panel (6), its characterized in that: the inside of carbon dioxide incubator (1) is provided with sliding plate (2), the inside of sliding plate (2) is provided with a set of culture dish (3), the inside and the outside of carbon dioxide incubator (1) are provided with fluid infusion subassembly (4), the inside of carbon dioxide incubator (1) is provided with flowing back subassembly (5), control panel (6) is installed in the outside of carbon dioxide incubator (1), timer (16) are installed in the outside of carbon dioxide incubator (1); the liquid draining assembly (5) comprises a waste liquid tank (501), a group of liquid draining pipes (502), a transverse pipe (503), a first hose (504) and a group of connecting covers (505), wherein the waste liquid tank (501) is fixedly connected to the inner bottom wall of the carbon dioxide incubator (1), the group of connecting covers (505) are respectively clamped into the inner parts of the group of clamping covers (7), the group of liquid draining pipes (502) respectively penetrate through and are fixedly connected to the inner parts of the group of connecting covers (505), the group of liquid draining pipes (502) respectively penetrate through and are fixedly connected to the inner parts of the transverse pipe (503), the first hose (504) penetrates through and is fixedly connected to the inner parts of the transverse pipe (503), and sealing rings are respectively embedded into the inner parts of the group of connecting covers (505); the liquid supplementing assembly (4) comprises a storage box (401), a water pump (402), a second hose (403), a shunt tube (404), a group of liquid supplementing pipes (405) and a group of metering valves (406), wherein the storage box (401) is fixedly connected to the outer side of the carbon dioxide incubator (1), the water pump (402) is installed to the outer side of the carbon dioxide incubator (1), a fixed pipe is fixedly connected to the inner part of the storage box (401), the input end of the water pump (402) is fixedly connected with the fixed pipe, the output end of the water pump (402) is fixedly connected with the second hose (403), the second hose (403) penetrates through and extends to the inner part of the carbon dioxide incubator (1), the second hose (403) penetrates through and is fixedly connected to the inner part of the shunt tube (404), and the group of metering valves (406) penetrate through and are respectively installed to the middle parts of the group of liquid supplementing pipes (405); the culture dish (3) comprises a dish body (301), a filter disc (302), a guide pipe (303) and an electromagnetic valve (304), wherein the filter disc (302) is fixedly connected to the inside of the dish body (301), the guide pipe (303) penetrates through and is fixedly connected to the inside of the dish body (301), the electromagnetic valve (304) is mounted at the middle part of the guide pipe (303), a clamping cover (7) communicated with the guide pipe (303) is fixedly connected to the bottom end of the guide pipe (303), the clamping cover (7) is embedded into the sliding plate (2), and the inner diameter of a filter hole of the filter disc (302) is 0.04mm; the bottom ends of the fluid infusion pipes (405) penetrate through and are fixedly connected with a protective box (13), and two small electron microscopes (14) are arranged on the outer sides of the protective boxes (13).
2. The efficient pig raising culture system for increasing the maturation rate of oocytes according to claim 1, wherein: the middle part fixedly connected with diaphragm (8) of a set of fluid-discharge tube (502), diaphragm (8) sliding connection is to the inside of carbon dioxide incubator (1), the outside fixedly connected with two springs (9) of diaphragm (8), two spring (9) are all fixedly connected with the inside of carbon dioxide incubator (1).
3. The efficient pig raising culture system for increasing the maturation rate of oocytes according to claim 1, wherein: two heating wires (10) are arranged in the storage box (401), and a temperature sensor (11) is arranged on the outer side of the storage box (401).
4. The efficient pig raising culture system for increasing the maturation rate of oocytes according to claim 1, wherein: two electric push rods (12) are fixedly connected inside the carbon dioxide incubator (1), and the bottom ends of the two electric push rods (12) are fixedly connected with the shunt tubes (404).
5. The efficient pig raising culture system for increasing the maturation rate of oocytes according to claim 1, wherein: the inside of control panel (6) is provided with central processing unit (15), the output of central processing unit (15) respectively with metering valve (406), solenoid valve (304), water pump (402), heating wire (10), carbon dioxide incubator (1) and electric putter (12) input signal connection, central processing unit (15) respectively with miniature electron microscope (14), time-recorder (16) and temperature sensor (11) two-way signal connection.
6. A method for raising pigs efficiently by increasing the maturation rate of oocytes, which is characterized by being applied to the culture system for raising pigs efficiently by increasing the maturation rate of oocytes according to any one of the claims 1-5, and comprises the following specific steps:
s1, respectively placing the prepared oocytes in a group of dish bodies (301) by researchers, then inserting a sliding plate (2) into the carbon dioxide incubator (1), and driving the carbon dioxide incubator (1) to operate by using a control panel (6);
s2, preheating the culture solution in the storage box (401) by using two heating wires (10), after the temperature reaches 38 ℃, enabling the temperature sensor (11) to receive detection data, then operating by using the water pump (402), leading out the culture solution from the storage box (401), then respectively discharging the culture solution into the inside of the dish body (301) by using the liquid supplementing pipe (405), and opening a metering valve (406) in the liquid supplementing process so as to ensure the quantitative discharge of the culture solution, then enabling the culture solution to be in contact with oocytes, and then stopping the operation of the water pump (402) and the metering valve (406);
s3, after the fluid infusion is completed, using two electric push rods (12) to operate to drive a small electron microscope (14) to operate to a preset position, using a timer (16) to perform timing of 4h, 8h and 12h by researchers, observing and recording the condition of the oocyte at intervals, using the small electron microscope (14) to intuitively observe the condition of the oocyte, and ensuring the culture environment of the oocyte without opening a carbon dioxide incubator (1);
s4, after 12 hours, the timer (16) sends data to the central processing unit (15), then the electromagnetic valve (304) is opened, so that the culture solution flows into the waste liquid tank (501) along a group of liquid discharge pipes (502), the culture solution level gradually drops, and the oocytes are conveniently blocked by the arrangement of the filter discs (302);
s5, the water pump (402) and the metering valve (406) are used for operating again, the culture solution is supplemented into the dish body (301), the culture solution replacement effect is achieved, the whole process is not required to be manually replaced by staff, meanwhile, oocytes are always located in the carbon dioxide incubator (1), a good culture environment is ensured, and then the oocytes can be taken out after being continuously cultured in the carbon dioxide incubator (1) for 16 hours.
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU1540197A (en) * | 1997-01-08 | 1998-08-03 | Demtek A/S | Method and apparatus for culturing cells and tissues |
| CN102154198A (en) * | 2010-12-16 | 2011-08-17 | 西北农林科技大学 | Simple in-vitro maturation culture method for oocytes |
| CN104789523A (en) * | 2015-05-18 | 2015-07-22 | 河南省格林金斯生物科技有限公司 | Simple, effective and low-consumption porcine oocyte in vitro mature cloning and culturing method |
| WO2016070454A1 (en) * | 2014-11-06 | 2016-05-12 | 徐小杨 | Automation device, carrier and method for oocyte in vitro fertilization and cleavage culture |
| CN209039501U (en) * | 2018-07-26 | 2019-06-28 | 侯志伟 | A kind of kit rapidly promoting human embryo's development |
| CN209652338U (en) * | 2018-12-29 | 2019-11-19 | 上海怡豪生物科技有限公司 | A kind of CAR-T cell culture apparatus |
| CN209854150U (en) * | 2019-04-15 | 2019-12-27 | 柳州市妇幼保健院 | Novel dynamic early embryo culture dish with culture solution updated in real time |
| CN110747160A (en) * | 2019-11-27 | 2020-02-04 | 浙江大学 | High-survival-rate sheep fertilized egg culture method for extra-embryonic-body culture |
| CN211420187U (en) * | 2019-06-11 | 2020-09-04 | 北京诺赛启研再生医学研究院有限公司 | Stem cell incubator with detection function |
| CN214830398U (en) * | 2020-12-30 | 2021-11-23 | 杭州恩格生物医疗科技有限公司 | Immune cell culture device |
-
2021
- 2021-11-25 CN CN202111409891.2A patent/CN114058506B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU1540197A (en) * | 1997-01-08 | 1998-08-03 | Demtek A/S | Method and apparatus for culturing cells and tissues |
| CN102154198A (en) * | 2010-12-16 | 2011-08-17 | 西北农林科技大学 | Simple in-vitro maturation culture method for oocytes |
| WO2016070454A1 (en) * | 2014-11-06 | 2016-05-12 | 徐小杨 | Automation device, carrier and method for oocyte in vitro fertilization and cleavage culture |
| CN104789523A (en) * | 2015-05-18 | 2015-07-22 | 河南省格林金斯生物科技有限公司 | Simple, effective and low-consumption porcine oocyte in vitro mature cloning and culturing method |
| CN209039501U (en) * | 2018-07-26 | 2019-06-28 | 侯志伟 | A kind of kit rapidly promoting human embryo's development |
| CN209652338U (en) * | 2018-12-29 | 2019-11-19 | 上海怡豪生物科技有限公司 | A kind of CAR-T cell culture apparatus |
| CN209854150U (en) * | 2019-04-15 | 2019-12-27 | 柳州市妇幼保健院 | Novel dynamic early embryo culture dish with culture solution updated in real time |
| CN211420187U (en) * | 2019-06-11 | 2020-09-04 | 北京诺赛启研再生医学研究院有限公司 | Stem cell incubator with detection function |
| CN110747160A (en) * | 2019-11-27 | 2020-02-04 | 浙江大学 | High-survival-rate sheep fertilized egg culture method for extra-embryonic-body culture |
| CN214830398U (en) * | 2020-12-30 | 2021-11-23 | 杭州恩格生物医疗科技有限公司 | Immune cell culture device |
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