CN113349312A - Anti-fogging bait for industrial blue crab culture and preparation method thereof - Google Patents

Anti-fogging bait for industrial blue crab culture and preparation method thereof Download PDF

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Publication number
CN113349312A
CN113349312A CN202110672394.5A CN202110672394A CN113349312A CN 113349312 A CN113349312 A CN 113349312A CN 202110672394 A CN202110672394 A CN 202110672394A CN 113349312 A CN113349312 A CN 113349312A
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parts
powder
bait
fogging
feed
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CN113349312B (en
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乔莹
马笑晚
陈旭阳
邢永泽
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Fourth Institute Of Oceanography Ministry Of Natural Resources
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Fourth Institute Of Oceanography Ministry Of Natural Resources
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
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    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
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    • A23KFODDER
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Abstract

The invention discloses an anti-fogging bait for industrial blue crab culture and a preparation method thereof, wherein the bait is prepared from the following raw materials in parts by weight: 0.8-1.2 parts of salt, 28-32 parts of minced fish, 14-16 parts of shrimp shell powder or crab shell powder, 5-7 parts of cuttlefish powder, 15-17 parts of peeled soybean meal, 1.8-2.2 parts of spirulina powder or algae residue, 19-21 parts of strong flour, 1.8-2.2 parts of monocalcium phosphate, 2.9-3.1 parts of fish oil, 2.9-3.1 parts of electrolytic multi-vitamin and 1.2-1.4 parts of phagostimulant; in the bait preparation process, minced fillet is pulped at low temperature and is fully gelatinized at 40-50 ℃ to form a gel structure, so that the feed has good stability in water and moderate hardness and is easy to bite by blue crabs. The crab feed is half-cooked feed after being cooked at 75 ℃, and is more suitable for being eaten by green crabs compared with completely cooked feed.

Description

Anti-fogging bait for industrial blue crab culture and preparation method thereof
Technical Field
The invention relates to the technical field of blue crab cultivation. More particularly, the invention relates to an anti-fogging bait for industrial blue crab cultivation and a preparation method thereof.
Background
The green crab is one of the marine culture products in China, and is deeply popular in the market all the time because the meat is tender, the taste is delicious, the nutrition is rich, and especially the crab paste is well-cooked after the female crab (commonly called as the paste crab) with mature gonad is cooked, and the color and luster of the crab paste is orange yellow or orange red.
The blue crabs mainly take small fishes, shrimps, crabs, shellfish and the like as baits, while natural biological baits not only destroy marine fishery resources, but also have limited resources, cannot ensure timely and sufficient supply, have single nutritional structure, and are easy to rot and decompose after being fed to destroy water quality. The existing commercial feed has richer nutrition, but has the problems of easy atomization and dispersion in water and poor stability after being soaked for a long time and being cut by the blue crab chela.
Disclosure of Invention
The invention aims to provide anti-fogging bait for industrial blue crab cultivation, which can balance nutrition, has good stability in water and is easy for the blue crab to bite.
The invention also aims to provide a preparation method of the anti-fogging bait for industrial blue crab cultivation, which is characterized in that minced fillet is fully gelatinized at 40-50 ℃ after low-temperature pulping, so as to form a gel structure, so that the feed has good stability in water, moderate hardness and is easy to bite blue crabs. The crab feed is half-cooked feed after being cooked at 75 ℃, and is more suitable for being eaten by green crabs compared with completely cooked feed.
To achieve these objects and other advantages in accordance with the present invention, there is provided an anti-fogging bait for industrial blue crab cultivation, which is prepared from the following components in parts by weight:
0.8-1.2 parts of salt, 28-32 parts of minced fish, 14-16 parts of shrimp shell powder or crab shell powder, 5-7 parts of cuttlefish powder, 15-17 parts of peeled soybean meal, 1.8-2.2 parts of spirulina powder or algae residue, 19-21 parts of strong flour, 1.8-2.2 parts of monocalcium phosphate, 2.9-3.1 parts of fish oil, 2.9-3.1 parts of electrolytic multi-vitamin and 1.2-1.4 parts of phagostimulant.
Preferably, in the anti-fogging bait for industrial blue crab cultivation, the preparation raw materials also comprise glutamine transaminase, and the dosage of the glutamine transaminase is one thousandth to four thousandth of the total amount of the raw materials.
Preferably, in the anti-fogging bait for industrial blue crab cultivation, the phagostimulant is prepared from glutamic acid, betaine and allicin in a mass ratio of 6: 6: 1.
The preparation method of the anti-fogging bait for industrial blue crab cultivation comprises the following steps:
grinding shrimp shell powder or crab shell powder, cuttlefish powder, peeled soybean meal powder, spirulina powder or algae residue, high gluten flour, calcium dihydrogen phosphate, electrolytic multivitamin and a phagostimulant into powder, and sieving with a sieve of 80-100 meshes;
step two, adding salt, fish oil and glutamine transaminase into minced trash fish, adding the raw material sieved in the step one, and fully pulping at 0-5 ℃ to obtain pulp;
and step three, molding the slurry prepared in the step two through a mold, then fully gelatinizing at 40-50 ℃, curing, drying, and freezing for storage.
Preferably, in the preparation method of the anti-fogging bait for industrial blue crab cultivation, in the second step, ice blocks with the weight of 10-15% of the pulp are added during pulping so as to be sufficiently pulped at 0-5 ℃, and in the third step, the ice blocks are aged at 75 ℃.
Preferably, in the preparation method of the anti-fogging bait for industrial blue crab cultivation, in the third step, the drying is freeze drying, and after curing, the step of uniformly wrapping the oyster shell wrappage before drying is further included, wherein the preparation method of the oyster shell wrappage comprises the following steps:
step one), uniformly mixing 300 times of pyroligneous liquor and EM bacterial liquid according to the mass ratio of 10:1 to obtain mixed liquor;
step two), mixing the activated oyster shell powder and the bacillus subtilis powder according to the mass ratio of 20: 1, uniformly mixing to obtain mixed powder;
step three), mixing the mixed powder, the mixed solution and the adhesive according to a mass ratio of 1: 2: 0.5, and evenly mixing to obtain the oyster shell wrappage.
Preferably, in the preparation method of the anti-fogging bait for industrial blue crab cultivation, the preparation method of the activated oyster shell powder comprises the following steps:
step A), soaking oyster shells in alkali liquor for 10 hours, filtering, washing with water, air-drying, and crushing to 60 meshes to obtain oyster shell coarse powder for later use;
step B), mixing the oyster shell coarse powder and far infrared ceramic powder according to a weight ratio of 250:1, then activating for 6 hours at the temperature of 200 ℃, and then crushing into 200 meshes to obtain activated oyster shell powder.
Preferably, in the preparation method of the anti-fogging bait for industrial blue crab cultivation, in the step A), oyster shells are soaked in a sodium hydroxide solution with the mass fraction of 20%.
Preferably, in the preparation method of the anti-fogging bait for industrial blue crab cultivation, the adhesive is composed of xanthan gum, sodium carboxymethylcellulose and sodium alginate according to a mass ratio of 2.5:1.5: 1.
The invention at least comprises the following beneficial effects:
the bait prepared by the invention has balanced nutrition, and the minced fillet is fully gelatinized at 40-50 ℃ after being pulped at low temperature in the preparation process to form a gel structure, so that the feed has good stability in water, has moderate hardness and is easy to bite by blue crabs. The crab feed is half-cooked feed after being cooked at 75 ℃, and is more suitable for being eaten by green crabs compared with completely cooked feed.
In the preparation process of the feed, the pyroligneous liquor, the EM bacterial liquid and the bacillus subtilis powder are introduced at normal temperature after curing, and freeze drying is adopted, so that the effective components in the pyroligneous liquor can be prevented from volatilizing, and the EM bacterial and the bacillus subtilis are inactivated. Through introducing the wood vinegar, the trace elements can be increased, the effects of sterilizing, disinfecting and regulating the health of the digestive tract of the blue crabs can be achieved, the shelf life of the feed can be prolonged, and the water quality can be purified. The EM bacterial liquid and the bacillus subtilis powder are added to condition the intestinal tracts of the crabs and improve the immunity of the blue crabs. The activated oyster shell powder is used as a slow-release material, so that the wood vinegar, EM (effective microorganisms) and bacillus subtilis can enter pores of the activated oyster shell powder, and the wood vinegar, EM (microorganisms) and bacillus subtilis can be prevented from completely entering a water body and being eaten by blue crabs due to the fact that the feed is soaked in the water for a long time.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.
It is to be noted that the experimental methods described in the following embodiments are all conventional methods unless otherwise specified, and the reagents and materials are commercially available unless otherwise specified.
The anti-fogging bait for industrial blue crab cultivation is prepared from the following raw materials in parts by weight:
0.8-1.2 parts of salt, 28-32 parts of minced fish, 14-16 parts of shrimp shell powder or crab shell powder, 5-7 parts of cuttlefish powder, 15-17 parts of peeled soybean meal, 1.8-2.2 parts of spirulina powder or algae residue, 19-21 parts of strong flour, 1.8-2.2 parts of monocalcium phosphate, 2.9-3.1 parts of fish oil, 2.9-3.1 parts of electrolytic multi-vitamin and 1.2-1.4 parts of phagostimulant.
In the anti-fogging bait for industrial blue crab cultivation, the preparation raw materials also comprise glutamine transaminase, and the dosage of the glutamine transaminase is one thousandth to four thousandth of the total amount of the raw materials.
In the anti-fogging bait for industrial blue crab cultivation, the phagostimulant is prepared from glutamic acid, betaine and allicin in a mass ratio of 6: 6: 1.
The preparation method of the anti-fogging bait for industrial blue crab cultivation comprises the following steps:
grinding shrimp shell powder or crab shell powder, cuttlefish powder, peeled soybean meal powder, spirulina powder or algae residue, high gluten flour, calcium dihydrogen phosphate, electrolytic multivitamin and a phagostimulant into powder, and sieving with a sieve of 80-100 meshes;
step two, adding salt, fish oil and glutamine transaminase into minced trash fish, adding the raw material sieved in the step one, and fully pulping at 0-5 ℃ to obtain pulp;
and step three, molding the slurry prepared in the step two through a mold, then fully gelatinizing at 40-50 ℃, curing, drying, and freezing for storage.
The bait prepared by the invention has balanced nutrition, and the minced fillet is fully gelatinized at 40-50 ℃ after being pulped at low temperature in the preparation process to form a gel structure, so that the feed has good stability in water, has moderate hardness and is easy to bite by blue crabs. The crab feed is half-cooked feed after being cooked at 75 ℃, and is more suitable for being eaten by green crabs compared with completely cooked feed.
In the preparation method of the anti-fogging bait for industrial blue crab cultivation, in the second step, ice blocks with the weight of 10-15% of the slurry are added during pulping so as to fully pulp at 0-5 ℃, and the third step is aging at 75 ℃.
In the preparation method of the anti-fogging bait for industrial blue crab cultivation, in the third step, the drying is freeze drying, and after curing, the step of uniformly coating the surfaces with oyster shell wrappings is further included before drying, wherein the preparation method of the oyster shell wrappings comprises the following steps:
step one), uniformly mixing 300 times of pyroligneous liquor and EM bacterial liquid according to the mass ratio of 10:1 to obtain mixed liquor;
step two), mixing the activated oyster shell powder and the bacillus subtilis powder according to the mass ratio of 20: 1, uniformly mixing to obtain mixed powder;
step three), mixing the mixed powder, the mixed solution and the adhesive according to a mass ratio of 1: 2: 0.5, and evenly mixing to obtain the oyster shell wrappage.
In the preparation process of the feed, the pyroligneous liquor, the EM bacterial liquid and the bacillus subtilis powder are introduced at normal temperature after curing, and freeze drying is adopted, so that the effective components in the pyroligneous liquor can be prevented from volatilizing, and the EM bacterial and the bacillus subtilis are inactivated. Through introducing the wood vinegar, the trace elements can be increased, the effects of sterilizing, disinfecting and regulating the health of the digestive tract of the blue crabs can be achieved, the shelf life of the feed can be prolonged, and the water quality can be purified. The EM bacterial liquid and the bacillus subtilis powder are added to condition the intestinal tracts of the crabs and improve the immunity of the blue crabs. The activated oyster shell powder is used as a slow-release material, so that the wood vinegar, EM (effective microorganisms) and bacillus subtilis can enter pores of the activated oyster shell powder, and the wood vinegar, EM (microorganisms) and bacillus subtilis can be prevented from completely entering a water body and being eaten by blue crabs due to the fact that the feed is soaked in the water for a long time.
In the preparation method of the anti-fogging bait for industrial blue crab cultivation, the preparation method of the activated oyster shell powder comprises the following steps:
step A), soaking oyster shells in alkali liquor for 10 hours, filtering, washing with water, air-drying, and crushing to 60 meshes to obtain oyster shell coarse powder for later use;
step B), mixing the oyster shell coarse powder and far infrared ceramic powder according to a weight ratio of 250:1, then activating for 6 hours at the temperature of 200 ℃, and then crushing into 200 meshes to obtain activated oyster shell powder.
In the preparation method of the anti-fogging bait for industrial blue crab cultivation, oyster shells are soaked in a sodium hydroxide solution with the mass fraction of 20% in the step A).
In the preparation method of the anti-fogging bait for industrial blue crab cultivation, the adhesive is composed of xanthan gum, sodium carboxymethylcellulose and sodium alginate according to a mass ratio of 2.5:1.5: 1.
In order to further improve the disease resistance of the blue crabs, the antiseptic and antibacterial ability of the feed and the stability in water, the technical scheme can also comprise the following technical details so as to better realize the technical effects:
in the step one), 300 times of pyroligneous liquor, curcumin, cyclodextrin and EM bacterial liquid are mixed according to the mass ratio of 10: 0.0006: 0.01: 1, uniformly mixing to obtain a mixed solution;
the binder was a hydrophobically modified xanthan gum, modified xanthan gum prepared in example 1 under application No. CN 201310694242.0.
Example 1
The anti-fogging bait for industrial blue crab cultivation is prepared from the following raw materials in parts by weight:
0.8 part of salt, 28 parts of minced fish, 14 parts of shrimp shell powder or crab shell powder, 5 parts of cuttlefish powder, 15 parts of peeled soybean meal, 1.8 parts of spirulina powder or algae residue, 19 parts of high gluten flour, 1.8 parts of monocalcium phosphate, 2.9 parts of fish oil, 2.9 parts of electrolytic multi-vitamin and 1.2 parts of phagostimulant.
In the anti-fogging bait for industrial blue crab cultivation, the preparation raw materials also comprise glutamine transaminase, and the dosage of the glutamine transaminase is one thousandth of the total amount of the raw materials.
In the anti-fogging bait for industrial blue crab cultivation, the phagostimulant is prepared from glutamic acid, betaine and allicin in a mass ratio of 6: 6: 1.
The preparation method of the anti-fogging bait for industrial blue crab cultivation comprises the following steps:
grinding shrimp shell powder or crab shell powder, cuttlefish powder, peeled soybean meal powder, spirulina powder or algae residue, high gluten flour, calcium dihydrogen phosphate, electrolytic multivitamin and a phagostimulant into powder, and sieving with a 80-mesh sieve;
step two, adding salt, fish oil and glutamine transaminase into minced trash fish, adding the raw material sieved in the step one, and fully pulping at 0 ℃ to obtain pulp;
and step three, molding the slurry prepared in the step two through a mold, then fully gelatinizing at 40 ℃, curing, drying and freezing for storage.
The bait prepared by the invention has balanced nutrition, and the minced fillet is fully gelatinized at 40 ℃ after being pulped at low temperature in the preparation process to form a gel structure, so that the feed has good stability in water, has moderate hardness and is easy to bite by blue crabs.
In the preparation method of the anti-fogging bait for industrial blue crab cultivation, in the second step, ice blocks with the weight of 10% of the pulp are added during pulping so as to fully pulp at 0 ℃, and in the third step, the ice blocks are cured at 75 ℃.
Example 2
The anti-fogging bait for industrial blue crab cultivation is prepared from the following raw materials in parts by weight:
1 part of salt, 30 parts of minced trash fish, 15 parts of shrimp shell powder or crab shell powder, 6 parts of cuttlefish powder, 16 parts of peeled soybean meal, 2 parts of spirulina powder or algae residue, 20 parts of high gluten flour, 2 parts of monocalcium phosphate, 3 parts of fish oil, 3 parts of electrolytic multi-vitamin and 1.3 parts of phagostimulant.
In the anti-fogging bait for industrial blue crab cultivation, the preparation raw materials also comprise glutamine transaminase, and the dosage of the glutamine transaminase is two thousandth of the total amount of the raw materials.
In the anti-fogging bait for industrial blue crab cultivation, the phagostimulant is prepared from glutamic acid, betaine and allicin in a mass ratio of 6: 6: 1.
The preparation method of the anti-fogging bait for industrial blue crab cultivation comprises the following steps:
grinding shrimp shell powder or crab shell powder, cuttlefish powder, peeled soybean meal powder, spirulina powder or algae residue, high gluten flour, calcium dihydrogen phosphate, electrolytic multivitamin and a phagostimulant into powder, and sieving with a 90-mesh sieve;
step two, adding salt, fish oil and glutamine transaminase into minced trash fish, adding the raw material sieved in the step one, and fully pulping at 2 ℃ to obtain pulp;
and step three, molding the slurry prepared in the step two through a mold, then fully gelatinizing at 45 ℃, curing, drying and freezing for storage.
The bait prepared by the invention has balanced nutrition, and the minced fillet is fully gelatinized at 45 ℃ after being pulped at low temperature in the preparation process to form a gel structure, so that the feed has good stability in water, has moderate hardness and is easy to bite by blue crabs.
In the preparation method of the anti-fogging bait for industrial blue crab cultivation, in the second step, ice blocks with the weight of 12% of the pulp are added during pulping so as to fully pulp at the temperature of 2 ℃, and in the third step, the ice blocks are cured at the temperature of 75 ℃.
Example 3
The anti-fogging bait for industrial blue crab cultivation is prepared from the following raw materials in parts by weight:
1.2 parts of salt, 32 parts of minced trash fish, 16 parts of shrimp shell powder or crab shell powder, 7 parts of cuttlefish powder, 17 parts of peeled soybean meal, 2.2 parts of spirulina powder or algae residue, 21 parts of strong flour, 2.2 parts of monocalcium phosphate, 3.1 parts of fish oil, 3.1 parts of electrolytic multi-vitamin and 1.4 parts of phagostimulant.
In the anti-fogging bait for industrial blue crab cultivation, the preparation raw materials also comprise glutamine transaminase, and the dosage of the glutamine transaminase is four thousandth of the total amount of the raw materials.
In the anti-fogging bait for industrial blue crab cultivation, the phagostimulant is prepared from glutamic acid, betaine and allicin in a mass ratio of 6: 6: 1.
The preparation method of the anti-fogging bait for industrial blue crab cultivation comprises the following steps:
grinding shrimp shell powder or crab shell powder, cuttlefish powder, peeled soybean meal powder, spirulina powder or algae residue, high gluten flour, calcium dihydrogen phosphate, electrolytic multivitamin and a phagostimulant into powder, and sieving with a 100-mesh sieve;
step two, adding salt, fish oil and glutamine transaminase into minced trash fish, adding the raw material sieved in the step one, and fully pulping at 5 ℃ to obtain pulp;
and step three, molding the slurry prepared in the step two through a mold, then fully gelatinizing at 50 ℃, curing, drying and freezing for storage.
The bait prepared by the invention has balanced nutrition, and the minced fillet is fully gelatinized at 50 ℃ after being pulped at low temperature in the preparation process to form a gel structure, so that the feed has good stability in water, has moderate hardness and is easy to bite by blue crabs.
In the preparation method of the anti-fogging bait for industrial blue crab cultivation, in the second step, ice blocks with the weight of 15% of the pulp are added during pulping so as to fully pulp at the temperature of 5 ℃, and in the third step, the ice blocks are cured at the temperature of 75 ℃.
Example 4
On the basis of embodiment 2, in the third step, the drying is freeze drying, and after aging, the method for preparing the anti-fogging bait for industrial blue crab cultivation further comprises the step of uniformly coating an oyster shell coating on the surface before drying, wherein the method for preparing the oyster shell coating comprises the following steps:
step one), uniformly mixing 300 times of pyroligneous liquor and EM bacterial liquid according to the mass ratio of 10:1 to obtain mixed liquor;
step two), mixing the activated oyster shell powder and the bacillus subtilis powder according to the mass ratio of 20: 1, uniformly mixing to obtain mixed powder;
step three), mixing the mixed powder, the mixed solution and the adhesive according to a mass ratio of 1: 2: 0.5, and evenly mixing to obtain the oyster shell wrappage.
In the preparation process of the feed, the pyroligneous liquor, the EM bacterial liquid and the bacillus subtilis powder are introduced at normal temperature after curing, and freeze drying is adopted, so that the effective components in the pyroligneous liquor can be prevented from volatilizing, and the EM bacterial and the bacillus subtilis are inactivated. Through introducing the wood vinegar, the trace elements can be increased, the effects of sterilizing, disinfecting and regulating the health of the digestive tract of the blue crabs can be achieved, the shelf life of the feed can be prolonged, and the water quality can be purified. The EM bacterial liquid and the bacillus subtilis powder are added to condition the intestinal tracts of the crabs and improve the immunity of the blue crabs. The activated oyster shell powder is used as a slow-release material, so that the wood vinegar, EM (effective microorganisms) and bacillus subtilis can enter pores of the activated oyster shell powder, and the wood vinegar, EM (microorganisms) and bacillus subtilis can be prevented from completely entering a water body and being eaten by blue crabs due to the fact that the feed is soaked in the water for a long time.
In the preparation method of the anti-fogging bait for industrial blue crab cultivation, the preparation method of the activated oyster shell powder comprises the following steps:
step A), soaking oyster shells in alkali liquor for 10 hours, filtering, washing with water, air-drying, and crushing to 60 meshes to obtain oyster shell coarse powder for later use;
step B), mixing the oyster shell coarse powder and far infrared ceramic powder according to a weight ratio of 250:1, then activating for 6 hours at the temperature of 200 ℃, and then crushing into 200 meshes to obtain activated oyster shell powder.
In the preparation method of the anti-fogging bait for industrial blue crab cultivation, oyster shells are soaked in a sodium hydroxide solution with the mass fraction of 20% in the step A).
In the preparation method of the anti-fogging bait for industrial blue crab cultivation, the adhesive is composed of xanthan gum, sodium carboxymethylcellulose and sodium alginate according to a mass ratio of 2.5:1.5: 1.
Example 5
On the basis of the embodiment 4, in order to further improve the disease resistance of the blue crabs, the antiseptic and antibacterial ability of the feed and the stability in water, the technical scheme can further comprise the following technical details so as to better realize the technical effects:
in the step one), 300 times of pyroligneous liquor, curcumin, cyclodextrin and EM bacterial liquid are mixed according to the mass ratio of 10: 0.0006: 0.01: 1, uniformly mixing to obtain a mixed solution; curcumin has antioxidant, antibacterial and immunity enhancing effects, but is insoluble in water. The cyclodextrin molecule has hydrophilic outer edge and hydrophobic inner cavity, so that it can provide one hydrophobic binding site as host to envelop various proper guest matters, and the cyclodextrin inner cavity can envelop curcumin to make curcumin soluble in water.
The binder was a hydrophobically modified xanthan gum, modified xanthan gum prepared in example 1 under application No. CN 201310694242.0. The feed is wrapped by the hydrophobic xanthan gum, so that the dissolution rate of effective ingredients in the feed in water can be reduced, the stability of the feed in water is better, and the utilization rate of the feed by the blue crabs is higher.
Comparative example 1
The anti-fogging bait for industrial blue crab cultivation is prepared from the following raw materials in parts by weight:
1 part of salt, 30 parts of fish meal, 15 parts of shrimp shell powder or crab shell powder, 6 parts of cuttlefish meal, 16 parts of peeled soybean meal, 2 parts of spirulina powder or algae residue, 20 parts of high gluten flour, 2 parts of monocalcium phosphate, 3 parts of fish oil, 3 parts of electrolytic multi-vitamin, 1 part of adhesive and 1.3 parts of phagostimulant.
In the anti-fogging bait for industrial blue crab cultivation, the phagostimulant is prepared from glutamic acid, betaine and allicin in a mass ratio of 6: 6: 1.
In the anti-fogging bait for industrial blue crab cultivation, the adhesive is composed of xanthan gum, sodium carboxymethylcellulose and sodium alginate according to a mass ratio of 2.5:1.5: 1.
The preparation method of the anti-fogging bait for industrial blue crab cultivation comprises the following steps:
grinding shrimp shell powder or crab shell powder, cuttlefish powder, peeled soybean meal powder, spirulina powder or algae residue, high gluten flour, calcium dihydrogen phosphate, electrolytic multi-vitamin, adhesive and phagostimulant into powder, sieving with 80 mesh sieve, mixing with fish oil, extruding into 3mm thick feed with a pellet feed granulator, cutting into 1-2cm hard pellet feed, steaming the feed in a steamer for 15min, oven drying at 50 deg.C, and freezing for storage.
Test 1
Carrying out industrial breeding tests on the blue crabs in a Hongshan harbor district of the northern sea city of the Zhuang nationality of Guangxi province, selecting 640 blue crabs with the weight of 101 +/-0.34 g, randomly distributing the blue crabs into 16 cement ponds, wherein 40 blue crabs are distributed in each pond, 4 cement ponds adopt the feeds prepared in the embodiments 2, 4 and 5 of the invention and the comparative example 1 to feed, 3 groups of 12 cement ponds are arranged for repeating, the blue crabs in each cement pond are fed for 2 times every day, the time is 8:30 and 16:30, water is changed every other day, and the water change amount is about 1/3 every time. During the experiment, the water temperature is 28-31 ℃, the dissolved oxygen is more than 5.0mg/L, and after 4 weeks, the blue crabs in each cement pond are counted and weighed, and the survival rate and the weight gain rate are calculated. The results are shown in Table 1.
TABLE 1 survival rate and weight gain rate of blue crabs
Figure BDA0003116242000000091
Figure BDA0003116242000000101
As can be seen from table 1, the survival rate of the blue crabs can be improved by feeding the feeds prepared in examples 2, 4 and 5, and the survival rate and the weight gain rate of the blue crabs are the highest by feeding the feeds prepared in example 5, which indicates that in the preparation process of the feeds, pyroligneous liquor, EM bacterial liquid and bacillus subtilis powder are introduced at normal temperature after curing, and freeze drying is adopted, so that volatilization of active ingredients in the pyroligneous liquor and inactivation of EM bacteria and bacillus subtilis can be avoided. Through introducing the wood vinegar, the trace elements can be increased, the effects of sterilizing, disinfecting and regulating the health of the digestive tract of the blue crabs can be achieved, the shelf life of the feed can be prolonged, and the water quality can be purified. The EM bacterial liquid and the bacillus subtilis powder are added to condition the intestinal tracts of the crabs and improve the immunity of the blue crabs. The activated oyster shell powder is used as a slow-release material, so that the wood vinegar, EM (effective microorganisms) and bacillus subtilis can enter pores of the activated oyster shell powder, and the wood vinegar, EM (microorganisms) and bacillus subtilis can be prevented from completely entering a water body and being eaten by blue crabs due to the fact that the feed is soaked in the water for a long time. And the loss rate of effective components in the feed in water can be reduced by adopting the hydrophobic xanthan gum, so that the feed has better stability in water, and the utilization rate of the feed by the blue crabs is higher. .
Test 2
In the fourth oceanic research institute laboratory of the department of natural resources, the feed prepared in examples 2, 4 and 5 was used for a soaking test, and a comparison was made by using a commercial granular feed for blue crabs (blue cloud crab puffed compound feed) and comparative example 1, and the conditions of atomization, dissolution and loss after the feed is put into water for 1 hour, the longest water-resistant time, and atomization, dispersion in water after being soaked for 6 hours, and the blue crabs are cut into big crabs are detected, and the results are shown in table 2, and the horizontal bar in table 2 indicates no dissolution and loss.
TABLE 2 feed entry conditions
Figure BDA0003116242000000102
Figure BDA0003116242000000111
As can be seen from table 2, the immersion test using the feeds prepared in examples 2, 4, and 5 showed no dissolution loss after 1 hour, no dispersion and no atomization in water after being immersed for 6 hours after being cut by the mitten crab, and the feed prepared in example 5 showed no dissolution loss and the longest water-resistant time, which indicates that the feed coated with hydrophobic xanthan gum can reduce the dissolution rate of the effective ingredients in the feed in water, so that the feed has better stability in water, and the utilization rate of the feed by the mitten crab is higher.
Test 3
The feeds prepared in inventive examples 2, 4, 5 and comparative example 1 were tested for storage time at 0 c and the results are shown in table 3.
TABLE 3 feed storage time
Group of Storage time/day
Example 2 10
Example 4 13
Example 5 20
Comparative example 1 7
As can be seen from table 3, the longest storage time at 0 ℃ using the feed prepared in example 5 indicates that wood vinegar and curcumin can be introduced during the preparation of the feed to achieve the effects of sterilization and disinfection, thereby improving the shelf life of the feed. And after the wood vinegar liquid is introduced, freeze drying is adopted, so that the volatilization of effective components in the wood vinegar liquid can be avoided, and the wood vinegar liquid can play a role for a long time.
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details given herein and to the embodiments shown and described without departing from the generic concept as defined by the claims and their equivalents.

Claims (9)

1. The anti-fogging bait for industrial blue crab cultivation is characterized in that the preparation raw materials comprise the following components in parts by weight:
0.8-1.2 parts of salt, 28-32 parts of minced fish, 14-16 parts of shrimp shell powder or crab shell powder, 5-7 parts of cuttlefish powder, 15-17 parts of peeled soybean meal, 1.8-2.2 parts of spirulina powder or algae residue, 19-21 parts of strong flour, 1.8-2.2 parts of monocalcium phosphate, 2.9-3.1 parts of fish oil, 2.9-3.1 parts of electrolytic multi-vitamin and 1.2-1.4 parts of phagostimulant.
2. The industrial blue crab culture anti-fogging bait according to claim 1, characterized in that the preparation raw materials further include glutamine transaminase in an amount of one to four thousandths of the total amount of the raw materials.
3. The industrial blue crab culture anti-fogging bait according to claim 1, wherein the phagostimulant is prepared from glutamic acid, betaine and allicin in a mass ratio of 6: 6: 1.
4. The method for preparing the anti-fogging bait for industrial blue crab cultivation according to claim 1, characterized by comprising the following steps:
grinding shrimp shell powder or crab shell powder, cuttlefish powder, peeled soybean meal powder, spirulina powder or algae residue, high gluten flour, calcium dihydrogen phosphate, electrolytic multivitamin and a phagostimulant into powder, and sieving with a sieve of 80-100 meshes;
step two, adding salt, fish oil and glutamine transaminase into minced trash fish, adding the raw material sieved in the step one, and fully pulping at 0-5 ℃ to obtain pulp;
and step three, molding the slurry prepared in the step two through a mold, then fully gelatinizing at 40-50 ℃, curing, drying, and freezing for storage.
5. The method for preparing an anti-fogging bait for industrial blue crab cultivation according to claim 4, wherein in the second step, ice cubes in an amount of 10-15% by weight of the slurry are added during the beating to sufficiently beat at 0-5 ℃, and in the third step, the ice cubes are aged at 75 ℃.
6. The method for preparing anti-fogging bait for industrial blue crab cultivation according to claim 4, wherein in the third step, the drying is freeze drying, and after aging, the method further comprises the step of uniformly wrapping the oyster shell wrappage before drying, wherein the method for preparing the oyster shell wrappage comprises the steps of:
step one), uniformly mixing 300 times of pyroligneous liquor and EM bacterial liquid according to the mass ratio of 10:1 to obtain mixed liquor;
step two), mixing the activated oyster shell powder and the bacillus subtilis powder according to the mass ratio of 20: 1, uniformly mixing to obtain mixed powder;
step three), mixing the mixed powder, the mixed solution and the adhesive according to a mass ratio of 1: 2: 0.5, and evenly mixing to obtain the oyster shell wrappage.
7. The method for preparing an anti-fogging bait for industrial blue crab cultivation according to claim 6, wherein the method for preparing the activated oyster shell powder comprises the steps of:
step A), soaking oyster shells in alkali liquor for 10 hours, filtering, washing with water, air-drying, and crushing to 60 meshes to obtain oyster shell coarse powder for later use;
step B), mixing the oyster shell coarse powder and far infrared ceramic powder according to a weight ratio of 250:1, then activating for 6 hours at the temperature of 200 ℃, and then crushing into 200 meshes to obtain activated oyster shell powder.
8. The method for preparing an anti-fogging bait for industrial blue crab cultivation according to claim 7, wherein in the step A), oyster shells are soaked in a sodium hydroxide solution with a mass fraction of 20%.
9. The method for preparing the anti-fogging bait for industrial blue crab cultivation according to claim 6, wherein the binder is composed of xanthan gum, sodium carboxymethylcellulose and sodium alginate in a mass ratio of 2.5:1.5: 1.
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