CN113349057A - Method for improving salt-tolerant growth capacity of forage grass seedlings in saline-alkali soil - Google Patents
Method for improving salt-tolerant growth capacity of forage grass seedlings in saline-alkali soil Download PDFInfo
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- CN113349057A CN113349057A CN202110784313.0A CN202110784313A CN113349057A CN 113349057 A CN113349057 A CN 113349057A CN 202110784313 A CN202110784313 A CN 202110784313A CN 113349057 A CN113349057 A CN 113349057A
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- 239000003513 alkali Substances 0.000 title claims abstract description 53
- 239000002689 soil Substances 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 38
- 230000012010 growth Effects 0.000 title claims abstract description 19
- 244000025254 Cannabis sativa Species 0.000 title claims description 19
- 239000004459 forage Substances 0.000 title description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 41
- 238000002791 soaking Methods 0.000 claims abstract description 25
- 239000011780 sodium chloride Substances 0.000 claims abstract description 24
- 150000003839 salts Chemical class 0.000 claims abstract description 20
- 230000035784 germination Effects 0.000 claims abstract description 15
- 238000004140 cleaning Methods 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 238000009331 sowing Methods 0.000 claims abstract description 9
- 238000001035 drying Methods 0.000 claims abstract description 7
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 30
- 239000000243 solution Substances 0.000 claims description 27
- 239000001963 growth medium Substances 0.000 claims description 24
- 238000005286 illumination Methods 0.000 claims description 23
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 18
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 18
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
- 238000012258 culturing Methods 0.000 claims description 18
- 239000001103 potassium chloride Substances 0.000 claims description 15
- 235000011164 potassium chloride Nutrition 0.000 claims description 15
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 14
- 239000001110 calcium chloride Substances 0.000 claims description 14
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 14
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
- 210000002257 embryonic structure Anatomy 0.000 claims description 12
- IOLCXVTUBQKXJR-UHFFFAOYSA-M potassium bromide Chemical compound [K+].[Br-] IOLCXVTUBQKXJR-UHFFFAOYSA-M 0.000 claims description 12
- 235000015097 nutrients Nutrition 0.000 claims description 9
- 239000011790 ferrous sulphate Substances 0.000 claims description 8
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 8
- 239000001509 sodium citrate Substances 0.000 claims description 8
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 8
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 7
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 7
- 239000012883 rooting culture medium Substances 0.000 claims description 7
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 6
- 239000004327 boric acid Substances 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 6
- 239000012869 germination medium Substances 0.000 claims description 4
- 239000012882 rooting medium Substances 0.000 claims description 4
- 239000012266 salt solution Substances 0.000 claims description 4
- 239000012267 brine Substances 0.000 claims description 2
- 238000003306 harvesting Methods 0.000 claims description 2
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims description 2
- 238000011161 development Methods 0.000 abstract description 4
- 230000018109 developmental process Effects 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 4
- 241001465754 Metazoa Species 0.000 abstract description 3
- 230000000052 comparative effect Effects 0.000 description 16
- 239000007788 liquid Substances 0.000 description 11
- 241000219823 Medicago Species 0.000 description 9
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 9
- 239000000463 material Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical group [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- -1 alkaline earth Substances 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000007726 management method Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 229910021653 sulphate ion Inorganic materials 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/002—Culture media for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Cultivation Of Plants (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for improving salt-tolerant growth capacity of pasture seedlings in saline-alkali soil, belonging to the technical field of agricultural planting, and comprising the following steps: obtaining seeds, soaking the seeds in a saline solution, electrifying, cleaning with saline water, drying, and sowing in a saline-alkali land area; the method for improving the salt-tolerant growth capacity of the pasture seedlings in the saline-alkali soil can fully provide the activity of pasture seeds, improve the germination condition of the pasture seedlings in the saline-alkali soil with high salt and high pH, improve the resistance and the adaptability of the seedlings to salt and alkali stress, effectively improve the soil quality of surrounding soil, reduce the alkalinity, further enhance the survival rate and the growth state of the pasture and promote the improvement of the saline-alkali soil environment and the development of animal husbandry.
Description
Technical Field
The invention relates to the technical field of agricultural planting, in particular to a method for improving salt-tolerant growth capacity of pasture seedlings in saline-alkali soil.
Background
The saline-alkali soil is a kind of salt accumulation, which means that the salt contained in the soil influences the normal growth of crops, and the alkaline earth and the alkaline soil are formed, most of the salt is related to the accumulation of carbonate in the soil, so the alkalization degree is generally high, and plants in a serious saline-alkali soil area can hardly survive. Saline-alkali soil is also called saline soil, and comprises saline soil, alkaline earth, saline soil and alkaline soil. Only when the salinity and the alkalization degree of the soil reach certain values, the soil is called saline soil and alkaline earth. In the process of utilizing the saline-alkali soil, the saline-alkali soil can be divided into light saline-alkali soil, moderate saline-alkali soil and heavy saline-alkali soil. The light saline-alkali soil means that the emergence rate is 70-80%, and the salt content is below three per thousand; the heavy saline-alkali soil has salt content more than six thousandths, and the emergence rate is lower than 50%; (the pH value is 7.1-8.5 for mild saline-alkali soil, 8.5-9.5 for moderate saline-alkali soil and above 9.5 for severe saline-alkali soil).
With the improvement of living standard, people's food hygiene consciousness is gradually strengthened, and green food is advocated, and in meat food consumption, the original pig and chicken mainly fattened by feed are thrown to the beef and mutton mainly fattened by forage, so that the livestock product mainly containing natural forage has wide development prospect. However, the existing farmland area is insufficient, and if animal husbandry is developed and land competition with grain and cotton is avoided, saline-alkali soil improvement and wide saline-alkali soil development are imperative. But due to the special environment of saline-alkali soil, the survival rate of the pasture is very low and the growth condition is poor. Therefore, how to improve the survival rate and the growth condition of the pasture in the saline-alkali soil is a key problem for developing and developing the saline-alkali soil at present.
Disclosure of Invention
The invention aims to provide a method for improving salt-tolerant growth capacity of pasture seedlings in saline-alkali soil, which aims to solve the problems in the prior art.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides a method for improving salt-tolerant growth capacity of pasture seedlings in saline-alkali soil, which comprises the following steps:
(1) seed harvesting
The method comprises the following steps of taking grass seeds, cleaning, soaking in ethanol at 30-40 ℃ for 8-15min, transferring into a pretreatment solution, and soaking for 2-3d, wherein the pretreatment solution comprises: 0.7 to 1.2 weight percent of calcium chloride, 0.5 to 0.8 weight percent of magnesium sulfate, 1.2 to 1.8mg/L of boric acid, 1.1 to 1.5 weight percent of potassium bromide and 1.3 to 1.6mg/L of IBA;
taking out the grass seeds, peeling off the episperms, taking out immature embryos, inoculating the immature embryos in a callus culture medium, and culturing the callus; after 25 days, transferring the seeds into a germination culture medium, and performing germination culture; when the height of the seedling is 2-3cm, transferring the seedling into a rooting culture medium, and performing rooting culture for 3-5 d; transferring the seeds into a water culture nutrient solution to culture until the seeds are mature, and obtaining the seeds;
(2) soaking the seeds obtained in the step (1) in a salt solution, electrifying, washing with saline water, and drying;
(3) and (3) sowing the seeds treated in the step (2) in a saline-alkali land area.
Further, the callus culture medium contains 3mg/L sodium citrate and 0.35-0.60 wt% sodium chloride.
Further, the callus was cultured in an incubator at 30 ℃ and with illumination intensity of 2000-.
Furthermore, the germination medium contains ferrous sulfate with the concentration of 2.5mg/L and calcium chloride with the concentration of 0.4-0.6 wt%.
Further, the germination culture is cultured in a constant temperature incubator with the temperature of 35-40 ℃, the illumination intensity of 3500-.
Further, the rooting medium contains potassium chloride with a concentration of 0.8-1.0 wt%, and sodium hydroxide is added to adjust the pH to 7.5-9.5.
Further, the rooting culture is carried out in a constant temperature incubator with the air humidity of less than 10%, the temperature of 35-40 ℃, the air flow rate of 3-5m/s and the illumination intensity of 2500-.
Further, the pH value of the water culture nutrient solution is 8.0-9.8, and the salt content is 1.1-1.5 wt%.
Further, the salt solution is a sodium chloride solution with the concentration of 3-5 wt%, and the soaking time is 30-60 s; the brine is a potassium chloride solution with the concentration of 0.5-0.8 wt%.
Further, the electrification treatment adopts pulse current with the voltage of 120-150V and the pulse interval of 0.3 s.
The invention discloses the following technical effects:
the method adopts the alcohol to soak the seeds, and then adopts a plurality of pretreatment liquids with high salt content to soak the seeds, and the alcohol soaking can protect the seeds from being forced by the pretreatment liquids with high salt content and improve the resistance of the seeds and plants grown by the seeds. The pretreatment liquid and the subsequent high-salt culture medium culture enable the seeds to be exercised in different stages, and the salt and alkali resistance is enhanced.
The grass seeds sowed by the invention are seeds formed by grass after callus culture, and the sowing is carried out by producing seeds and sowing again, so that the workload of cultivation is greatly reduced under the condition of basically not reducing the indexes of next generation seeds. The seeds formed by the first-generation pasture cultured by the callus can recover the salt and alkali resistance of the second-generation seeds only by simple saline soaking and electrifying treatment, so that the seeds have almost the same performance as the first-generation pasture and have very high recovery capability.
The method for improving the salt-tolerant growth capacity of the pasture seedlings in the saline-alkali soil can fully improve the activity of pasture seeds, improve the germination condition of the pasture seedlings in the saline-alkali soil with high salt and high pH value, and improve the resistance and the adaptability of the seedlings to salt and alkali stress, can effectively improve the soil quality of surrounding soil, reduce the alkalinity, further enhance the survival rate and the growth state of the pasture, and has a promoting effect on improving the saline-alkali soil environment and the development of animal husbandry.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The description and examples are intended to be illustrative only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.
In the present invention, the callus culture medium, rooting culture medium, germination culture medium or hydroponic culture medium can be any commercially available culture medium or culture medium, and as in the following examples, if not described, the normal components of each culture medium can be MS culture medium, and the present invention is not limited to the basic components thereof as long as it has basic nutrients necessary for sufficient plant growth.
Example 1
A method for improving salt-tolerant growth capacity of pasture seedlings in saline-alkali soil comprises the following steps:
(1) the method comprises the following steps of taking grass seeds, cleaning, soaking in ethanol at 35 ℃ for 10min, transferring into pretreatment liquid, and soaking for 2d, wherein the pretreatment liquid comprises: 0.9 wt% of calcium chloride, 0.7 wt% of magnesium sulfate, 1.5mg/L of boric acid, 1.3 wt% of potassium bromide and 1.5mg/L of IBA;
(2) taking out grass seeds, peeling off episperms, taking out immature embryos, inoculating the immature embryos in a callus culture medium containing 3mg/L sodium citrate and 0.50 wt% of sodium chloride, and culturing in a constant-temperature incubator at the temperature of 30 ℃ and the illumination intensity of 2500 lx;
(3) after 25 days, transferring the seeds into a germination culture medium containing ferrous sulfate with the concentration of 2.5mg/L and calcium chloride with the concentration of 0.5 wt%, and culturing in a constant-temperature incubator with the temperature of 38 ℃, the illumination intensity of 3700lx and the illumination time of 14 h/d;
(4) when the height of the seedling is 2.5cm, transferring the seedling into a rooting culture medium containing 0.9 wt% of potassium chloride, adding sodium hydroxide to adjust the pH value to 8.5, and culturing for 4d in a constant-temperature incubator with the air humidity of less than 10%, the temperature of 38 ℃, the air flow rate of 4m/s and the illumination intensity of 2700 lx;
(5) transferring into water culture nutrient solution with pH of 9.2 and salt content of 1.3 wt% for culturing until the seeds are mature, and obtaining seeds;
(6) soaking harvested seeds in a 4 wt% sodium chloride solution, performing electrification treatment by adopting pulse current with the voltage of 135V and the pulse interval of 0.3s, then cleaning with a 0.6 wt% potassium chloride solution, and drying;
(7) and (4) sowing the seeds treated in the step (6) in the saline-alkali land area.
Example 2
A method for improving salt-tolerant growth capacity of pasture seedlings in saline-alkali soil comprises the following steps:
(1) the method comprises the following steps of taking grass seeds, cleaning, soaking in ethanol at 35 ℃ for 10min, transferring into pretreatment liquid, and soaking for 3d, wherein the pretreatment liquid contains: 0.7 wt% of calcium chloride, 0.8 wt% of magnesium sulfate, 1.2mg/L of boric acid, 1.5 wt% of potassium bromide and 1.3mg/L of IBA;
(2) taking out grass seeds, peeling off episperms, taking out immature embryos, inoculating the immature embryos in a callus culture medium containing 3mg/L sodium citrate and 0.60 wt% of sodium chloride, and culturing in a constant-temperature incubator at the temperature of 30 ℃ and the illumination intensity of 2000 lx;
(3) after 25 days, transferring the seeds into a germination culture medium containing ferrous sulfate with the concentration of 2.5mg/L and calcium chloride with the concentration of 0.6 wt%, and culturing in a constant-temperature incubator with the temperature of 35 ℃, the illumination intensity of 4000lx and the illumination time of 14 h/d;
(4) when the height of the seedling is 2cm, transferring the seedling into a rooting culture medium containing 1.0 wt% of potassium chloride, adding sodium hydroxide to adjust the pH to 7.5, and culturing for 3d in a constant-temperature incubator with the air humidity of less than 10%, the temperature of 40 ℃, the air flow rate of 3m/s and the illumination intensity of 3000 lx;
(5) transferring into water culture nutrient solution with pH of 8.0 and salt content of 1.5 wt% for culturing until the seeds are mature, and obtaining seeds;
(6) soaking harvested seeds in a 3 wt% sodium chloride solution, performing electrification treatment by adopting pulse current with the voltage of 150V and the pulse interval of 0.3s, then cleaning with a 0.5 wt% potassium chloride solution, and drying;
(7) and (4) sowing the seeds treated in the step (6) in the saline-alkali land area.
Example 3
A method for improving salt-tolerant growth capacity of pasture seedlings in saline-alkali soil comprises the following steps:
(1) the method comprises the following steps of taking grass seeds, cleaning, soaking in ethanol at 35 ℃ for 10min, transferring into pretreatment liquid, and soaking for 2d, wherein the pretreatment liquid comprises: 1.2 wt% of calcium chloride, 0.5 wt% of magnesium sulfate, 1.8mg/L of boric acid, 1.1 wt% of potassium bromide and 1.6mg/L of IBA;
(2) taking out grass seeds, peeling off episperms, taking out immature embryos, inoculating the immature embryos in a callus culture medium containing 3mg/L sodium citrate and 0.35 wt% of sodium chloride, and culturing in a constant-temperature incubator at the temperature of 30 ℃ and the illumination intensity of 3000 lx;
(3) transferring the seeds into a germination culture medium containing ferrous sulfate with the concentration of 2.5mg/L and 0.4 wt% of calcium chloride after 25d, and culturing in a constant-temperature incubator at the temperature of 40 ℃, the illumination intensity of 3500lx and the illumination time of 14 h/d;
(4) when the height of the seedling is 3cm, transferring the seedling into a rooting culture medium containing 0.8 wt% of potassium chloride, adding sodium hydroxide to adjust the pH to 9.5, and culturing for 5d in a constant-temperature incubator with the air humidity of less than 10%, the temperature of 35 ℃, the air flow rate of 5m/s and the illumination intensity of 2500 lx;
(5) transferring into water culture nutrient solution with pH of 9.8 and salt content of 1.1 wt% for culturing until the seeds are mature, and obtaining seeds;
(6) soaking harvested seeds in a 5 wt% sodium chloride solution, performing electrification treatment by adopting a pulse current with the voltage of 120V and the pulse interval of 0.3s, then cleaning with a 0.8 wt% potassium chloride solution, and drying;
(7) and (4) sowing the seeds treated in the step (6) in the saline-alkali land area.
Example 4
A method for improving salt-tolerant growth capacity of pasture seedlings in saline-alkali soil comprises the following steps:
(1) the method comprises the following steps of taking grass seeds, cleaning, soaking in ethanol at 35 ℃ for 10min, transferring into pretreatment liquid, and soaking for 2d, wherein the pretreatment liquid comprises: 1.1 wt% of calcium chloride, 0.7 wt% of magnesium sulfate, 1.7mg/L of boric acid, 1.2 wt% of potassium bromide and 1.2mg/L of IBA;
(2) taking out grass seeds, peeling off episperms, taking out immature embryos, inoculating the immature embryos in a callus culture medium containing 3mg/L sodium citrate and 0.5 wt% of sodium chloride, and culturing in a constant-temperature incubator at the temperature of 30 ℃ and the illumination intensity of 2300 lx;
(3) after 25 days, transferring the seeds into a germination culture medium containing ferrous sulfate with the concentration of 2.5mg/L and calcium chloride with the concentration of 0.5 wt%, and culturing in a constant-temperature incubator with the temperature of 37 ℃, the illumination intensity of 3600lx and the illumination time of 14 h/d;
(4) when the height of the seedling is 3cm, transferring the seedling into a rooting culture medium containing 0.9 wt% of potassium chloride, adding sodium hydroxide to adjust the pH to 9.2, and culturing for 4d in a constant-temperature incubator with the air humidity of less than 10%, the temperature of 37 ℃, the air flow rate of 3.5m/s and the illumination intensity of 2900 lx;
(5) transferring into water culture nutrient solution with pH of 8.5 and salt content of 1.4 wt% for culturing until the seeds are mature, and obtaining seeds;
(6) soaking harvested seeds in a 4 wt% sodium chloride solution, performing electrification treatment by adopting pulse current with the voltage of 140V and the pulse interval of 0.3s, then cleaning with a 0.7 wt% potassium chloride solution, and drying;
(7) and (4) sowing the seeds treated in the step (6) in the saline-alkali land area.
Comparative example 1
The difference from the example 1 is that the grass seeds are directly sown in saline-alkali land areas without any treatment.
Comparative example 2
The difference from the embodiment 1 is that the method does not comprise the steps (1) to (5), but directly takes the grass seeds, and seeds are sowed in the saline-alkali land area after the grass seeds are treated in the step (6).
Comparative example 3
The difference from example 1 is that the pretreatment solution of step (1) contained IBA1.2mg/L only.
Comparative example 4
The difference from example 1 is that the callus culture medium of step (2) does not contain sodium citrate and sodium chloride.
Comparative example 5
The difference from example 1 is that the germination medium of step (3) does not contain ferrous sulfate and calcium chloride.
Comparative example 6
The difference from example 1 is that the rooting medium of step (4) does not contain potassium chloride and has a pH of 7.5.
Comparative example 7
The difference from example 1 is that step (6) is not subjected to energization treatment.
Comparative example 8
The difference from example 1 is that sodium citrate and sodium chloride in the callus culture medium of step (2) were replaced with sodium sulfate and potassium chloride at the same concentrations.
Comparative example 9
The difference from example 1 is that the ferrous sulphate and calcium chloride in the germination medium of step (3) are replaced by the same concentration of ferric sulphate and sodium chloride.
Comparative example 10
The difference from example 1 is that potassium chloride in the rooting medium of step (4) was replaced with sodium chloride at the same concentration and pH 8.5.
Comparative example 11
The difference from example 1 is that in step (1), ethanol soaking is not employed.
Effect verification
According to the methods of the examples 1-4 and the comparative examples 1-11, alfalfa is planted in a saline-alkali land area (the original pH of the saline-alkali land is 9.37, the total salt content is 0.91%), a common water and fertilizer management method is adopted after planting, the germination condition of alfalfa seeds 14d is recorded, when the alfalfa is harvested, the biomass increasing rate of the alfalfa in each example and comparative example is calculated according to the mass of the same number of alfalfa, and the calculation method is as follows: the alfalfa quality of the examples or comparative examples to be tested/alfalfa quality of comparative example 1 is 100% -1, and then the pH of the soil near the alfalfa roots is measured, and the results are shown in table 1.
TABLE 1
As can be seen from Table 1, the above factors all have significant influence on the germination condition and growth condition of alfalfa seeds in saline-alkali soil areas, and can effectively reduce the pH value of the saline-alkali soil and improve the condition of the saline-alkali soil. In addition, although the comparative example 11 is less effective than example 1, in the actual treatment process, if ethanol soaking is not adopted, many bad species are generated, callus cannot be formed, and death during the culture process of the steps (2) to (4) is greatly increased by about 30%.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (10)
1. A method for improving salt-tolerant growth capacity of pasture seedlings in saline-alkali soil is characterized by comprising the following steps:
(1) seed harvesting
The method comprises the following steps of taking grass seeds, cleaning, soaking in ethanol at 30-40 ℃ for 8-15min, transferring into a pretreatment solution, and soaking for 2-3d, wherein the pretreatment solution comprises: 0.7 to 1.2 weight percent of calcium chloride, 0.5 to 0.8 weight percent of magnesium sulfate, 1.2 to 1.8mg/L of boric acid, 1.1 to 1.5 weight percent of potassium bromide and 1.3 to 1.6mg/L of IBA;
taking out the grass seeds, peeling off the episperms, taking out immature embryos, inoculating the immature embryos in a callus culture medium, and culturing the callus; after 25 days, transferring the seeds into a germination culture medium, and performing germination culture; when the height of the seedling is 2-3cm, transferring the seedling into a rooting culture medium, and performing rooting culture for 3-5 d; transferring the seeds into a water culture nutrient solution to culture until the seeds are mature, and obtaining the seeds;
(2) soaking the seeds obtained in the step (1) in a salt solution, electrifying, washing with saline water, and drying;
(3) and (3) sowing the seeds treated in the step (2) in a saline-alkali land area.
2. The method of claim 1, wherein the callus culture medium comprises sodium citrate at a concentration of 3mg/L and 0.35-0.60 wt% sodium chloride.
3. The method as claimed in claim 3, wherein the callus is cultured in an incubator at 30 ℃ and illumination intensity of 2000-3000 lx.
4. The method as claimed in claim 1, wherein the germination medium contains ferrous sulfate at a concentration of 2.5mg/L and 0.4-0.6 wt% calcium chloride.
5. The method as claimed in claim 4, wherein the germination culture is carried out in a constant temperature incubator at 35-40 ℃ with an illumination intensity of 3500 and 4000lx and an illumination time of 14 h/d.
6. The method according to claim 1, wherein the rooting medium contains potassium chloride in a concentration of 0.8-1.0 wt% and sodium hydroxide is added to adjust the pH to 7.5-9.5.
7. The method as claimed in claim 1, wherein the rooting culture is carried out in a constant temperature incubator with an air humidity of less than 10%, a temperature of 35-40 ℃, an air flow rate of 3-5m/s, and an illumination intensity of 2500-.
8. The method of claim 1, wherein the hydroponic nutrient solution has a pH of 8.0-9.8 and a salt content of 1.1-1.5 wt%.
9. The method according to claim 1, wherein the salt solution is a sodium chloride solution with a concentration of 3-5 wt%, and the soaking time is 30-60 s; the brine is a potassium chloride solution with the concentration of 0.5-0.8 wt%.
10. The method as claimed in claim 1, wherein the energizing process employs a pulse current with a voltage of 120-150V and a pulse interval of 0.3 s.
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