CN113324981A - Full-automatic chemiluminescent inspection device - Google Patents
Full-automatic chemiluminescent inspection device Download PDFInfo
- Publication number
- CN113324981A CN113324981A CN202110625815.9A CN202110625815A CN113324981A CN 113324981 A CN113324981 A CN 113324981A CN 202110625815 A CN202110625815 A CN 202110625815A CN 113324981 A CN113324981 A CN 113324981A
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- Prior art keywords
- reaction tube
- optical detection
- seat
- inspection device
- light shielding
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- 238000007689 inspection Methods 0.000 title claims abstract description 18
- 238000001514 detection method Methods 0.000 claims abstract description 114
- 238000006243 chemical reaction Methods 0.000 claims abstract description 100
- 230000003287 optical effect Effects 0.000 claims abstract description 65
- 239000007788 liquid Substances 0.000 claims abstract description 37
- 230000005284 excitation Effects 0.000 claims abstract description 31
- 238000002347 injection Methods 0.000 claims abstract description 24
- 239000007924 injection Substances 0.000 claims abstract description 24
- 210000001503 joint Anatomy 0.000 claims abstract description 15
- 238000007789 sealing Methods 0.000 claims abstract description 4
- 238000009434 installation Methods 0.000 claims description 13
- 230000035945 sensitivity Effects 0.000 abstract description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 3
- 238000003032 molecular docking Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 230000017525 heat dissipation Effects 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 238000002038 chemiluminescence detection Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
Abstract
The invention discloses a chemiluminescence full-automatic inspection device which comprises an optical detection chamber and a reaction tube, wherein one side of the optical detection chamber is provided with an optical detection unit, the other side of the optical detection chamber is provided with a reaction tube conveying unit, and the optical detection chamber is also provided with an excitation liquid injection unit for injecting excitation liquid into the reaction tube; and a shading component capable of moving up and down is arranged between the optical detection unit and the optical detection chamber, so that when the optical detection unit detects reaction light emitted by the reaction tube, the shading component and the reaction tube conveying unit form sealing butt joint matching. The invention realizes the characteristics of compact structure, small volume, convenient deployment, low cost, flexible detection and suitability for basic equipment on the premise of meeting the requirements of full automation and high detection sensitivity.
Description
Technical Field
The invention relates to a chemiluminescence detection technology, in particular to a chemiluminescence full-automatic detection device.
Background
The traditional full-automatic chemiluminescence test equipment has the advantages of high flux and full automation, but also exposes a short plate which is large in volume, high in cost, inflexible in the face of the detection requirement of a single sample and has higher requirements on installation and use environments, and provides new requirements for the test equipment along with the conversion of medical modes and the sinking of the requirements of in vitro diagnosis industries, such as: the method is convenient to operate, low in cost, and suitable for equipment deployment of basic-level medical institutions, flexible sample inspection, follow-up inspection and the like. In the face of new requirements, the traditional medium and large-scale computers do not have related advantages.
Disclosure of Invention
In order to solve the defects in the prior art, the invention aims to provide a chemiluminescence full-automatic inspection device which has the characteristics of compact structure, small volume, convenience in deployment, low cost, flexibility in detection and suitability for basic-level equipment on the premise of meeting the requirements of full automation and high detection sensitivity.
The technical scheme adopted by the invention for solving the technical problems is as follows: a chemiluminescence full-automatic inspection device comprises an optical detection chamber and a reaction tube, wherein one side of the optical detection chamber is provided with an optical detection unit, the other side of the optical detection chamber is provided with a reaction tube conveying unit, and the optical detection chamber is also provided with an exciting liquid injection unit for injecting exciting liquid into the reaction tube;
and a shading component capable of moving up and down is arranged between the optical detection unit and the optical detection chamber, so that when the optical detection unit detects reaction light emitted by the reaction tube, the shading component and the reaction tube conveying unit form sealing butt joint matching.
Optionally, the excitation liquid injection unit is disposed on the light shielding assembly, so that the excitation liquid injection unit moves up and down along with the light shielding assembly.
Optionally, the reaction tube conveying unit includes a first movable seat and a linear guide rail, the linear guide rail horizontally penetrates through the optical detection chamber, and the first movable seat is inserted into or removed from the optical detection chamber through the linear guide rail;
the first movable seat is provided with a reaction tube placing area for placing a reaction tube, so that the reaction tube is positioned in the reaction tube placing area.
Optionally, the reaction tube placing area is provided with a butt joint part matched with the shading component, so that the shading component is in butt joint with the butt joint part of the reaction tube placing area to form a closed space, and the reaction tube is located in the closed space.
Optionally, the light shielding assembly includes a second movable seat moving up and down, and the second movable seat is slidably connected to the top wall of the optical detection chamber;
the lateral parts of the two sides of the reaction tube placing area are provided with first grooves matched with the second moving seat, and the two sides of the second moving seat are provided with raised strips matched with the first grooves respectively.
Optionally, the reaction tube placement area includes a second groove disposed on a side of the first movable seat, a fixed seat is fixedly connected inside the second groove, and a height of a top of the fixed seat is lower than a height of a top surface of the first movable seat, so that a step is formed between the top surface of the fixed seat and the second groove;
the top surface of the fixed seat is provided with an installation cavity for installing the reaction tube, so that the reaction tube is vertically installed in the installation cavity;
the first grooves are formed in two sides of the fixing seat.
Optionally, a shading seat used for being matched with the step is arranged on the second movable seat, so that the shading seat completely covers the top surface of the fixed seat after being matched with the step.
Optionally, the light shielding assembly further includes a first detection hole, a second detection hole, and a third detection hole;
the first detection hole is formed in the second movable seat and penetrates through the surface of the second movable seat;
the second detection hole is formed in the outer side of the fixed seat and penetrates through the side part of the fixed seat from the inner wall of the mounting cavity;
the third detection hole is arranged between the optical detection chamber and the optical detection unit;
the first detection hole, the second detection hole and the third detection hole are consistent in specification, and when the shading assembly is in butt joint with the first movable seat, the first detection hole, the second detection hole and the third detection hole are distributed coaxially.
Optionally, the excitation liquid injection unit is installed on the light shielding seat, so that when the light shielding assembly is matched with the first movable seat, the excitation liquid injection unit is inserted into the reaction tube.
Optionally, the top of the reaction tube is exposed out of the top of the installation cavity, and a third groove matched with the part, exposed out of the installation cavity, of the reaction tube is formed in the bottom of the shading seat.
By adopting the technical scheme, the liquid path system, the movement mechanism, the optical detection chamber and the optical detection unit which are related to the reaction tube are integrated into a whole device, full-automatic detection can be realized, and the device has the advantages of high integration, small volume, compact structure, reliable operation and high detection sensitivity.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the structure of FIG. 1 without the cylinder and reservoir installed;
FIG. 3 is a schematic perspective view of an optical detection chamber according to the present invention;
FIG. 4 is a schematic perspective view of the optical detection chamber of the present invention;
FIG. 5 is a front view of the optical detection chamber of the present invention;
FIG. 6 is a third schematic perspective view of the optical detection chamber of the present invention.
Detailed Description
The present application will be described in further detail with reference to the following drawings and examples. It is to be understood that the specific embodiments described herein are merely illustrative of the relevant invention and not restrictive of the invention. It should be noted that, for convenience of description, only the portions related to the present invention are shown in the drawings.
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present application will be described in detail below with reference to the embodiments with reference to the attached drawings.
As shown in fig. 1 to 6, the present invention discloses a chemiluminescent full-automatic inspection device, which comprises an optical detection chamber 1 and a reaction tube 2, wherein an optical detection unit 3 is further disposed on one side of the optical detection chamber 1, and the optical detection unit 3 is used for detecting light emitted by a reactant in the reaction tube 2 after a chemical reaction. The other side of the optical detection chamber 1 is provided with a reaction tube transfer unit 4, and the reaction tube transfer unit 4 is used for feeding or taking the reaction tube 2 into or out of the optical detection chamber 1, so that a cavity 101 for accommodating the reaction tube transfer unit 4 is further provided inside the optical detection chamber 1. The optical detection chamber 1 is further provided with an excitation liquid injection unit 5 for injecting an excitation liquid into the reaction tube 2, and after the reaction tube transfer unit 4 feeds the reaction tube into the cavity 101 of the optical detection chamber 1, the excitation liquid injection unit 5 injects the excitation liquid into the reaction tube 2, so that the reactant in the reaction tube 2 undergoes a chemiluminescent reaction.
In the invention, the light shielding component 6 which can move up and down is arranged between the optical detection unit 3 and the optical detection chamber 1, so that when the optical detection unit 3 detects the reaction light emitted by the reaction tube 2, the light shielding component 6 and the reaction tube transmission unit 4 form a sealed butt-joint fit, therefore, it can be understood that after the light shielding component 6 and the reaction tube transmission unit 4 form a sealed butt-joint fit, a dark chamber is formed among the light shielding component 6, the reaction tube transmission unit 4 and the cavity 101, namely, the reaction tube 2 is positioned in the dark chamber, thereby preventing the interference of external light, and improving the sensitivity and detection accuracy of the optical detection unit 3. In one embodiment of the present invention, the optical detection unit 3 may employ a PMT photon detector.
In the present invention, the excitation liquid injection unit 5 may be disposed on the light shielding assembly 6, so that the excitation liquid injection unit 5 moves up and down along with the light shielding assembly 6. Therefore, when the light shielding member 6 moves downward, the excitation liquid injection unit 5 moves downward and is inserted into the reaction tube 2, and when the light shielding member 6 moves upward, the excitation liquid injection unit 5 moves upward and is removed from the reaction tube 2.
Specifically, the excitation liquid injection unit 5 includes an excitation liquid storage tank 501, an excitation liquid pipeline 502, and an injection head 503, wherein the injection head 503 is fixedly mounted on the shading assembly 6, and the injection head 503 is connected to the excitation liquid storage tank 501 through the excitation liquid pipeline 502, it should be noted that the excitation liquid storage tank 501 has a three-way valve for controlling the excitation liquid pipeline 502 to have liquid or not. In addition, as shown in fig. 2 to 6, the light shielding assembly 6 includes a second movable seat 601 moving up and down, the second movable seat 601 is slidably connected to the top wall of the optical detection chamber 1, the light shielding assembly 6 can be driven by the cylinder 7, the base of the cylinder 7 is fixedly mounted on the side wall of the optical detection chamber 1, meanwhile, the shaft body of the output shaft of the cylinder 7 is fixedly connected to the light shielding assembly 6, so that the light shielding assembly 6 moves up and down by the cylinder 7, and the excitation liquid storage tank 501 can be fixedly mounted at the end of the output shaft of the cylinder 7, so that the excitation liquid storage tank 501 also moves synchronously with the light shielding assembly 6, thereby reducing the usage amount of the liquid pipeline, reducing the rinsing amount of the excitation liquid, and further improving the accuracy of the usage amount of the excitation liquid.
In the present invention, as shown in FIG. 2, the reaction tube transfer unit 4 includes a first movable base 401 and a linear guide 402, wherein the linear guide 402 horizontally penetrates the optical detection chamber 1, and the first movable base 401 is inserted into or removed from the optical detection chamber 1 through the linear guide 402. When the linear guide 402 is specifically arranged, in order to prevent light leakage from the gap between the linear guide 402 and the first movable seat 401 and influence the detection of the optical detection unit 3, the linear guide 402 can be laid on one side of the cavity 101 away from the optical detection unit 3, so that light leaking from the gap between the linear guide 402 and the first movable seat 401 of the reaction tube 2 can be blocked by the first movable seat 401, and cannot be reflected to the position of the reaction tube 2, and the detection precision of the optical detection unit 3 is further improved. Therefore, a reaction tube placing region 403 for placing the reaction tube 2 may be provided on the first movable seat 401 such that the reaction tube 2 is positioned within the reaction tube placing region.
In the invention, the reaction tube placing area is provided with a butt joint part matched with the shading component 6, so that the shading component 6 is in butt joint with the butt joint part of the reaction tube placing area to form a closed space, the closed space is a darkroom, and the reaction tube 2 is positioned in the closed space. In addition, as shown in fig. 2 to 6, in order to facilitate the reaction tube placing area to be matched with the light shielding assembly 6, first grooves 403 matched with the second movable seat 601 may be disposed on two side portions of the reaction tube placing area, and protruding strips 602 respectively matched with the first grooves 403 may be disposed on two sides of the second movable seat 601.
Specifically, as shown in fig. 2 to 6, the reaction tube placement area includes a second groove 404 disposed at a side portion of the first movable seat 401, a fixed seat 405 is fixedly connected to an inside of the second groove 404, and a top height of the fixed seat 405 is lower than a top height of the first movable seat 401, so that a step is formed between the top surface of the fixed seat 405 and the second groove 404, and the step is a butt joint portion. An installation cavity for installing the reaction tube 2 is formed in the top surface of the fixing base 405, so that the reaction tube 2 is vertically installed in the installation cavity. In addition, in this state, the first grooves 403 are disposed on both sides of the fixed base 405, and when the shade assembly 6 moves downward, the protruding strips 602 of the second movable base 601 are inserted into the first grooves 403 from top to bottom and move downward along the first grooves 403.
In the present invention, a light shielding seat 603 for engaging with the step is disposed on the second movable seat 601, so that the light shielding seat 603 completely covers the top surface of the fixed seat 405 after engaging with the step. Therefore, when the light shielding seat 603 is engaged with the step, the light shielding seat 603 can complete the docking operation with the docking portion, thereby sealing the docking portion to form a closed space (i.e., a dark room). Since the light shielding seat 603 corresponds to the reaction tube 2, the injection head 503 can be further fixedly installed at the bottom of the light shielding seat 603, and the position of the injection head 503 corresponds to the position of the reaction tube 2, so that the injection head 503 is inserted into the reaction tube 2 after the light shielding seat 603 is matched with the butting portion.
In the present invention, the light shielding assembly 6 further includes a first detection hole 604, a second detection hole 605 and a third detection hole 606; the first probe hole 604 is disposed on the second movable base 601, and the first probe hole 604 penetrates through the surface of the second movable base 601; the second detection hole 605 is arranged on the outer side of the fixed seat 405, and the second detection hole 605 penetrates out of the side part of the fixed seat 405 from the inner wall of the installation cavity; the third detection hole 606 is provided between the optical detection chamber 1 and the optical detection unit 3. The first detection hole 604, the second detection hole 605 and the third detection hole 606 have the same specification, and when the light shielding assembly 6 is in butt joint with the first movable seat 401, the first detection hole 604, the second detection hole 605 and the third detection hole 606 are coaxially distributed.
In the present invention, the top of the reaction tube 2 is exposed from the top of the installation cavity, so that the reaction tube 2 can be filled with reagents and an exciting liquid. The bottom of the light shielding seat 603 is provided with a third groove (not shown) adapted to the portion of the reaction tube 2 exposed from the mounting cavity.
In the present invention, the cavity 101 of the optical detection chamber 1 may be configured as a detection cavity and a reaction cavity, wherein a filling pipeline system of a reagent or a cleaning solution is disposed in the reaction cavity, and after the reagent is filled into the reaction tube 2, the reagent reacts in the reaction tube 2. Since heat is generated during the chemical reaction, as shown in fig. 1, a peltier device 406 is further disposed on the back of the first movable base 401 to dissipate heat from the reaction tube 2, and thus, in the present invention, the first movable base 401 is made of a heat conductive material. In addition, a heat dissipation fan 407 may be further installed at the position of the reaction chamber, and the peltier 406 is matched with the heat dissipation fan 407, so that the reaction tube 2 can be rapidly cooled to prevent the temperature from affecting the detection of the optical detection unit 3.
The above description is only a preferred embodiment of the application and is illustrative of the principles of the technology employed. It will be appreciated by a person skilled in the art that the scope of the invention as referred to in the present application is not limited to the embodiments with a specific combination of the above-mentioned features, but also covers other embodiments with any combination of the above-mentioned features or their equivalents without departing from the inventive concept. For example, the above features may be replaced with (but not limited to) features having similar functions disclosed in the present application.
Other technical features than those described in the specification are known to those skilled in the art, and are not described herein in detail in order to highlight the innovative features of the present invention.
Claims (10)
1. The full-automatic chemiluminescence inspection device is characterized by comprising an optical detection chamber and a reaction tube, wherein one side of the optical detection chamber is provided with an optical detection unit, the other side of the optical detection chamber is provided with a reaction tube conveying unit, and the optical detection chamber is also provided with an excitation liquid injection unit for injecting excitation liquid into the reaction tube;
and a shading component capable of moving up and down is arranged between the optical detection unit and the optical detection chamber, so that when the optical detection unit detects reaction light emitted by the reaction tube, the shading component and the reaction tube conveying unit form sealing butt joint matching.
2. The chemiluminescent fully automatic inspection device according to claim 1 wherein the excitation liquid injection unit is disposed on the light shield assembly such that the excitation liquid injection unit moves up and down along with the light shield assembly.
3. The chemiluminescent fully automatic inspection device according to claim 2, wherein the reaction tube transfer unit comprises a first moving seat and a linear guide rail, the linear guide rail horizontally penetrates through the optical detection chamber, and the first moving seat is inserted into or removed from the optical detection chamber through the linear guide rail;
the first movable seat is provided with a reaction tube placing area for placing a reaction tube, so that the reaction tube is positioned in the reaction tube placing area.
4. The chemiluminescent fully automatic inspection device according to claim 3, wherein the reaction tube placing area is provided with a butt joint part matched with the light shielding component, so that the light shielding component and the butt joint part of the reaction tube placing area are butted to form a closed space, and the reaction tube is positioned in the closed space.
5. The chemiluminescent fully automatic inspection device according to claim 4, wherein the light shielding assembly comprises a second movable seat moving up and down, the second movable seat is connected with the top wall of the optical detection chamber in a sliding way;
the lateral parts of the two sides of the reaction tube placing area are provided with first grooves matched with the second moving seat, and the two sides of the second moving seat are provided with raised strips matched with the first grooves respectively.
6. The chemiluminescent fully automatic inspection device according to claim 5, wherein the reaction tube placement area comprises a second groove arranged on the side of the first movable seat, a fixed seat is fixedly connected inside the second groove, and the top height of the fixed seat is lower than the top height of the first movable seat, so that a step is formed between the top surface of the fixed seat and the second groove;
the top surface of the fixed seat is provided with an installation cavity for installing the reaction tube, so that the reaction tube is vertically installed in the installation cavity;
the first grooves are formed in two sides of the fixing seat.
7. The chemiluminescent fully automatic inspection device according to claim 6, wherein the second movable seat is provided with a light shielding seat for forming a fit with the step, so that the light shielding seat completely covers the top surface of the fixed seat after forming a fit with the step.
8. The chemiluminescent fully automated inspection device according to claim 7 wherein the light shield assembly further comprises a first detection aperture, a second detection aperture and a third detection aperture;
the first detection hole is formed in the second movable seat and penetrates through the surface of the second movable seat;
the second detection hole is formed in the outer side of the fixed seat and penetrates through the side part of the fixed seat from the inner wall of the mounting cavity;
the third detection hole is arranged between the optical detection chamber and the optical detection unit;
the first detection hole, the second detection hole and the third detection hole are consistent in specification, and when the shading assembly is in butt joint with the first movable seat, the first detection hole, the second detection hole and the third detection hole are distributed coaxially.
9. The chemiluminescent fully automatic inspection device according to claim 8 wherein the excitation liquid injection unit is mounted on the light shielding seat such that the excitation liquid injection unit is inserted into the reaction tube when the light shielding assembly is mated with the first movable seat.
10. The chemiluminescent fully automatic inspection device according to claim 9, wherein the top of the reaction tube is exposed out of the top of the installation cavity, and the bottom of the light shielding seat is provided with a third groove matched with the part of the reaction tube exposed out of the installation cavity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110625815.9A CN113324981B (en) | 2021-06-04 | 2021-06-04 | Chemiluminescence full-automatic inspection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110625815.9A CN113324981B (en) | 2021-06-04 | 2021-06-04 | Chemiluminescence full-automatic inspection device |
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| Publication Number | Publication Date |
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| CN113324981A true CN113324981A (en) | 2021-08-31 |
| CN113324981B CN113324981B (en) | 2023-11-07 |
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| CN202110625815.9A Active CN113324981B (en) | 2021-06-04 | 2021-06-04 | Chemiluminescence full-automatic inspection device |
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| US20170191936A1 (en) * | 2014-07-21 | 2017-07-06 | Technogenetics Holdings S.R.L. | Method and apparatus for chemiluminescence and/or fluorescence measuring |
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| CN113324981B (en) | 2023-11-07 |
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