CN113318129A

CN113318129A - Efficient antibacterial honey paste and preparation method thereof

Info

Publication number: CN113318129A
Application number: CN202110704794.XA
Authority: CN
Inventors: 王亚培; 潘乾灏; 陶行磊
Original assignee: Renmin University of China
Current assignee: Renmin University of China
Priority date: 2021-06-24
Filing date: 2021-06-24
Publication date: 2021-08-31

Abstract

The invention discloses a high-efficiency antibacterial honey paste and a preparation method thereof, and the preparation method comprises the following steps: 1) mixing honey and cellulose in proportion; 2) uniformly mixing the product obtained in the step 1) at a certain rotating speed and a certain temperature; 3) cooling and packaging. The high-efficiency antibacterial honey paste is free of any chemical additive, green, safe, free of toxic and side effects, capable of being directly used for a human body, excellent in antibacterial property, simple and effective in preparation method, capable of avoiding complex preparation process, capable of being effectively adhered to the skin and any wound surface, and capable of achieving long-term antibacterial effect, and in addition, the honey paste can be extruded through ointment, and convenient to store and use.

Description

Efficient antibacterial honey paste and preparation method thereof

Technical Field

The invention relates to a high-efficiency antibacterial honey paste and a preparation method thereof, belonging to the field of antibacterial and anti-inflammatory medicines.

Background

Honey is widely accepted in academic and medical fields as an excellent antibacterial agent, but at present, in the field of medical care, honey is usually mixed with other substances such as traditional Chinese medicines, food materials, fruits, food additives and the like and is used as an auxiliary material for various medical care products. Because the content of the honey is too low, the antibacterial effect of the honey is difficult to show, and even more, some products are sterilized at high temperature before use, so that the effective components of the honey are seriously damaged, and the antibacterial effect of the honey is lost. Therefore, no antibacterial product taking honey as a main material is directly sold in the market.

The flowability of honey greatly limits the direct application of honey, making it difficult to apply to human skin and surfaces of articles, and it is common to apply it as a dressing, for example, CN201821963150.2 makes a patch with honey, or CN201711458706.2 pours honey into hydrogel. However, the products all face similar problems, namely the structure is complex, the preparation is difficult, the price is high, the storage is difficult, the curative effect is poor, and therefore, the honey prepared into the dressing product is only seen on paper, and no commodity is seen. Recently, there are some products such as CN201711396730.8, which mix honey with thickener and additive to increase its viscosity and make it non-flowing, but the viscosity is too large to limit its application range, and more importantly, the content of honey used in these products is generally less than 50%, and the thickener is difficult to ensure its biological safety, so the curative effect of these products is still low and it is difficult to pass the GMP certification of medicine. There are also devices, such as patent CN201220477701.0, which prepare special toothpaste tube to contain honey, but this kind of product still does not solve the problem that honey is too thin and difficult to stay in the wound for a long time. Therefore, the development of an antibacterial honey ointment without toxic and side effects for the medical field is still a challenge.

Disclosure of Invention

The invention aims to provide a high-efficiency antibacterial honey paste which can be prepared into ointments, plasters and other pastes.

The honey paste provided by the invention is prepared from honey and cellulose,

wherein, the mass ratio of the honey to the cellulose can be 100: 30-100: 0.1;

the honey is selected from common commercially available honey including but not limited to manuka honey;

such celluloses include, but are not limited to, bacterial cellulose, bamboo fiber, pea fiber, sugar cane fiber, and the like.

The honey paste is prepared by the method comprising the following steps:

1) mixing honey and cellulose in proportion;

2) uniformly mixing the product obtained in the step 1) at a certain rotating speed and temperature;

3) cooling and packaging to obtain the product.

In the above preparation method, the honey is selected from common commercially available honey including, but not limited to, manuka honey.

A large number of experimental researches find that the New Zealand wheat kalu honey has good effect.

In the above preparation method, the cellulose includes, but is not limited to, bacterial cellulose, bamboo fiber, pea fiber, sugar cane fiber, etc.

In the step 1), the mass ratio of the honey to the bacterial cellulose can be as follows: 100: 30-100: 0.1, specifically 100: 0.1-100: 10, more particularly 100: 0.1, 100: 1 or 100: 10.

in step 2), the rotation speed may be: 100r/min-4000r/min, the temperature can be 30-50 ℃.

The honey ointment and salve prepared from the honey paste also belong to the protection scope of the invention.

The invention also protects the application of the honey paste as an antibacterial and anti-inflammatory medicine.

Compared with the prior art, the invention has the following advantages:

(1) the honey paste prepared by the invention has a simple preparation method and excellent antibacterial and anti-inflammatory effects.

(2) The honey paste obtained by the invention has no fluidity at normal body temperature, and can be used for wounds, pustules, acnes, burns, skin transplantation recovery and other parts.

(3) The honey paste obtained by the invention can be extruded through the paste, and is convenient to store and use.

(4) The honey paste obtained by the invention has no chemical additive, is green and safe, has no toxic or side effect, and can be directly used for human bodies.

Drawings

Fig. 1 shows the results of the bacteriostasis experiment of the honey paste prepared in example 1.

Fig. 2 shows the bacteriostatic test results of the honey paste prepared in example 2.

Fig. 3 shows the bacteriostatic test results of the honey paste prepared in example 3.

Fig. 4 is a result of a bacteriostatic experiment of the honey paste prepared in comparative example 1.

Fig. 5 shows the results of the bacteriostatic experiment of the honey paste prepared in comparative example 2.

Detailed Description

The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.

Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.

The invention provides a honey paste which is prepared from honey and cellulose,

wherein the mass ratio of honey to cellulose is 100: 30-100: 0.1.

the honey paste is prepared by the method comprising the following steps:

1) mixing honey and cellulose in proportion;

3) cooling and packaging to obtain the product.

The fluidity was measured in the following examples: a certain amount of product (such as 5g) is placed on a table top, and after 24, whether the product has shape change or not is judged, wherein no shape change is no fluidity, and the product having shape change is fluidity. The determination method of the antibacterial experiment comprises the following steps: a quantity of product (e.g., 2g) is placed in a petri dish containing bacteria. After the honey is placed for 24 days, the growth condition of bacteria around the honey is observed, and the larger the circle that the bacteria do not grow is, the better the bacteriostasis effect is.

Example 1

(1) The raw materials used in the honey paste in the embodiment comprise the following components by mass:

honey: manuka honey 100 parts

0.1 part of bacterial cellulose

(2) The preparation steps are as follows:

mixing Mel and bacterial cellulose at the above ratio, uniformly mixing at 500r/min and 40 deg.C, cooling, and packaging into ointment.

Example 2

The same procedure as in example 1, wherein the ratio of honey to bacterial cellulose was 100: 1.

example 3

The same procedure as in example 1, wherein the ratio of honey to bacterial cellulose was 100: 10.

comparative example 1

The same procedure as in example 1, wherein the ratio of honey to bacterial cellulose was 100: 0.05.

comparative example 2

The same procedure as in example 1, wherein the ratio of honey to bacterial cellulose was 100: 40.

the properties of the honey paste obtained by the above experiment are shown in table 1, and the antibacterial property is characterized at the same time: it can be seen that the ratio of honey to bacterial cellulose is 100: 0.1 to 1000: when the content of the bacterial cellulose is more than 10, the honey is difficult to have no fluidity because the content of the bacterial cellulose is too small, and the honey has no sterilization effect when the content of the bacterial cellulose is too much, so that the following components are not recommended to be used:

table 1: the performance of the paste prepared by different proportions of honey and bacterial cellulose

Sample (I)	Example 1	Example 2	Example 3	Comparative example 1	Comparative example 2
						Fluidity test	No fluidity	No fluidity	No fluidity	Has fluidity	No fluidity
Sterilizing effect	Is excellent in	Is excellent in	Is excellent in	Is excellent in	Is free of

Examples 4,

Example 4 similar to the experimental procedure of example 1, wherein honey was replaced in sequence with currently marketed honey, the resulting material remained free of flowability, had a slightly reduced bactericidal effect, and did not affect other properties.

Examples 5,

Example 5 similar to the experimental procedure of example 1, wherein the cellulose is bamboo fiber, the obtained material still has no flowability, the sterilization effect is unchanged, and other properties are not affected.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims

1. A honey paste is prepared from honey and cellulose,

wherein the mass ratio of honey to cellulose is 100: 30-100: 0.1.

2. a honey paste according to claim 1, characterized in that: in the honey paste, the mass ratio of honey to bacterial cellulose is as follows: 100: 0.1-100: 10.

3. a honey paste according to claim 1 or 2, characterized in that: in the honey paste, the cellulose is one or a mixture of more of bacterial cellulose, bamboo fiber, pea fiber and sugarcane fiber.

4. A method of making a honey paste of any one of claims 1-3, comprising the steps of:

1) mixing honey and cellulose in proportion;

3) cooling and packaging to obtain the product.

5. The method of claim 4, wherein:

in step 2), the rotating speed is as follows: 100r/min-4000r/min, the temperature is 30-50 ℃.

6. A honey ointment or salve prepared from a honey cream as claimed in any one of claims 1 to 3.

7. Use of a honey cream according to any one of claims 1 to 3, or a honey ointment or salve according to claim 6, as an antibacterial and anti-inflammatory medicament.