CN110898038A - Shark peptide gel coating agent and preparation method thereof - Google Patents
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Abstract
A shark peptide gel film coating agent and a preparation method thereof, relating to the field of biological medicine. The composite material comprises the following components in percentage by mass: 1.0 to 10.0 percent of shark peptide; 2.0 to 20.0 percent of gel film forming agent; 5.0 to 10.0 percent of penetration enhancer; 0.1 to 1.0 percent of antibacterial agent; 0 to 30.0 percent of absolute ethyl alcohol; 0.2 to 0.5 percent of tween-80; the balance of distilled water. The preparation method comprises the following steps: soaking the gel film-forming agent overnight, and dissolving to obtain a mucilage A; dissolving shark peptide in distilled water to obtain a solution B, and mixing the solution B with the mucilage A to obtain a solution C; adding absolute ethyl alcohol, tween-80 and a penetration enhancer into the solution C to obtain a solution D; and adding an antibacterial agent and distilled water into the solution D, and stirring to obtain the shark peptide gel film coating agent. The gel film coating agent can form a transparent film with soft texture, certain thickness, high elasticity and toughness in a short time on skin, is convenient to use, is not limited by the shape and the position of a skin wound surface, is suitable for nursing dry wounds and wounds with trace amount of exudates, and has the effects of moisturizing, inhibiting bacteria, promoting wound healing and the like.
Description
Technical Field
The invention relates to the field of biological medicines, in particular to a shark peptide gel film coating agent and a preparation method thereof.
Background
Dry wound healing has the following disadvantages: the wound is dehydrated and scabbed, which is not beneficial to the division and growth of epithelial cells; the bioactive substances are easy to lose, and the healing speed is slow; the dressing is adhered to the wound surface, and the patient feels pain when the dressing is replaced; it is easy to cause secondary mechanical damage. In conjunction with modern wound healing theory, wet healing may overcome the above-mentioned related disadvantages. The treatment of dry wounds, wet environments, and wound treatment agents have become a focus of research.
The hydrogel material can provide a moist and slightly acidic healing environment, has the advantages of protecting a wound surface, relieving wound pain, promoting granulation tissue growth, dissolving black scab and necrotic tissues, filling wounds such as sinus and lacuna and the like, is easily soaked by being applied too much, has low adhesive force and needs secondary dressing for fixation. The film coating agent is an external liquid preparation which is formed into a film after dissolving or dispersing raw material medicines in a solvent containing a film forming material and applying the film on an affected part, and has the advantages of simple preparation process, convenient use, strong adhesive force, no need of secondary dressing fixation, difficult falling, attractive appearance and the like, but compared with hydrogel, the film coating agent has relatively limited drug loading rate and poorer moisturizing performance.
Disclosure of Invention
The invention aims to solve the problems in the prior art, and provides a shark peptide gel film coating agent and a preparation method thereof, which are suitable for the adjuvant therapy of various exposed dry wounds and wounds with trace exudate, have the effects of water resistance, bacteriostasis, wound healing promotion and the like, are simple in preparation method, and are suitable for industrial production.
In order to achieve the purpose, the invention adopts the following technical scheme:
a shark peptide gel coating agent comprises the following components in percentage by mass: 1.0-10.0% of shark peptide; 2.0 to 20.0 percent of gel film forming agent; 5.0 to 10.0 percent of penetration enhancer; 0.1 to 1.0 percent of antibacterial agent; 0 to 30.0 percent of absolute ethyl alcohol; 0.2 to 0.5 percent of tween-80; the balance of distilled water.
The gel film forming agent is one or more of polyvinyl alcohol PVA-124, polyvinyl alcohol PVA-217, polyvinyl alcohol PVA-1788, polyvinyl alcohol PVA-0588, polyvidone, CMC-Na, polyhexamethylene glycol PEG-400, carbomer 940, Arabic gum, polyvinyl formal acetal and polyvinyl butyral.
The penetration enhancer comprises 1, 2-propylene glycol and glycerol.
The mass ratio of the 1, 2-propylene glycol to the glycerol is 1 (1-3).
The antibacterial agent comprises one or two of chlorhexidine gluconate and nisin.
A shark peptide gel coating agent comprises the following components in percentage by mass: 3.0 to 6.0 percent of shark peptide; 5.0 to 15.0 percent of gel film forming agent; 8.0 to 10.0 percent of penetration enhancer; 0.2 to 0.5 percent of antibacterial agent; 5.0 to 15.0 percent of absolute ethyl alcohol; tween-80, 0.2%; the balance of distilled water.
A preparation method of a shark peptide gel coating agent comprises the following steps:
1) soaking the gel film forming agent in distilled water or ethanol solution overnight, then placing the gel film forming agent in water bath for full dissolution, stirring the gel film forming agent to be uniform and naturally cooling the gel film forming agent to obtain gel slurry A;
2) fully dissolving shark peptide in distilled water to obtain a solution B, mixing the solution B with the mucilage A prepared in the step 1), and uniformly stirring at a low speed to obtain a solution C;
3) respectively and sequentially adding absolute ethyl alcohol, tween-80 and a penetration enhancer into the solution C prepared in the step 2), and sufficiently and uniformly stirring at a low speed to obtain a solution D;
4) and (3) adding a compatible antibacterial agent into the solution D obtained in the step (3), adding distilled water to a sufficient amount, stirring at a low speed to be uniform, completely defoaming, and filling to obtain the shark peptide gel film coating agent.
The temperature of the water bath in the step 1) is 75-90 ℃; the mixing temperature in the step 2) is 40-55 ℃.
In the invention, the shark peptide is obtained by washing shark meat and fish skin, degreasing, carrying out compound enzymolysis, carrying out high-speed centrifugation, carrying out fractional filtration and drying, and is a small molecular peptide which is independently researched and developed, and the preparation method is disclosed in the patent application number: 201810556221.5A Siraitia grosvenorii Swingle mixed polypeptide, its preparation method and application, and reference [1] Siraitia grosvenorii Swingle skin antioxidant polypeptide preparation and performance analysis), the variety source is one of Chilossyllium planiosum, Mustelus griseus, Prionice glauca.
Compared with the prior art, the technical scheme of the invention has the following beneficial effects:
the shark peptide is a small molecular shark peptide which is independently researched and produced, and has the characteristics of moisture preservation, oxidation resistance, free radical removal, inflammation diminishing, whitening, bacteriostasis, wound healing promotion and the like through biochemical and cytological detection. The novel gel coating agent is prepared by utilizing the relevant excellent characteristics of the shark peptide and combining with a gel film forming material, can be used for wound nursing of operation wounds and chronic ulcer surfaces, and is particularly suitable for repairing irregular wounds and joint bending parts.
The invention has reasonable compatibility, can form a transparent film with soft texture, certain thickness, elasticity and toughness in 5-10 min on the surface of the skin at room temperature after being smeared, is convenient to use, is not limited by the shape and the position of the wound surface of the skin, is suitable for nursing dry wounds with trace amount of exudates, has the effects of moisturizing, inhibiting bacteria, promoting wound healing and the like, has good adhesiveness on the surface of the skin, is safe and has no irritation.
In addition, the preparation method is simple, convenient to operate and suitable for industrial large-scale production.
Detailed Description
In order to make the technical problems, technical solutions and advantageous effects to be solved by the present invention clearer and more obvious, the present invention is further described in detail below with reference to the following embodiments.
The present invention can provide both types of ethanol-containing and ethanol-free formulations.
Example 1
1. In embodiment 1 of the invention, a shark peptide gel coating agent (ethanol-free formula) comprises the following components in percentage by mass:
shark peptide, 6.0%; 7.0 percent of polyvinyl alcohol PVA-217; polyvinyl alcohol PVA-0588, 3.0%; 6.0 percent of glycerin; 1, 2-propanediol, 2.0%; nisin, 0.02%; tween-80, 0.3%; the balance of distilled water.
2. The preparation method of the shark peptide gel coating agent with the mixture ratio comprises the following steps:
1) soaking the polyvinyl alcohol PVA-217 and the polyvinyl alcohol PVA-0588 in a proper amount of distilled water overnight, then fully dissolving in a water bath at about 90 ℃, stirring to be uniform to obtain a mucilage A, and naturally cooling;
2) fully dissolving the prescription amount of the megalosymus serpyllum in a proper amount of distilled water to obtain a solution B, mixing the solution B with the mucilage A prepared in the step 1), and uniformly stirring at a low speed at a temperature of 45 ℃ to obtain a solution C;
3) adding the tween-80, the 1, 2-propylene glycol and the glycerol in the formula amount into the solution C prepared in the step 2) slowly in sequence, and stirring the mixture fully and uniformly at a low speed to obtain a solution D;
4) adding an antibacterial agent nisin into the solution D obtained in the step 3, then adding distilled water to a sufficient amount, stirring at a low speed to be uniform, and filling after thorough defoaming to obtain the shark peptide gel film coating agent.
Example 2
1. In embodiment 2 of the invention, a shark peptide gel coating agent comprises the following components in percentage by mass:
shark peptide, 3.0%; 8.0 percent of polyvinyl alcohol PVA-217; polyvinyl alcohol PVA-0588, 2.0%; 5.0% of glycerol; 1, 2-propanediol, 2.5%; absolute ethyl alcohol, 6.0%; tween-80, 0.2%; the balance of distilled water.
2. The preparation method of the shark peptide gel coating agent with the mixture ratio comprises the following steps:
1) soaking the polyvinyl alcohol PVA-217 and the polyvinyl alcohol PVA-0588 in a proper amount of distilled water overnight, then fully dissolving in a water bath at about 90 ℃, stirring to be uniform to obtain a mucilage A, and naturally cooling;
2) fully dissolving the prescription amount of the megalosymus serpyllum in a proper amount of distilled water to obtain a solution B, mixing the solution B with the mucilage A prepared in the step 1), and uniformly stirring at a low speed at a temperature of 45 ℃ to obtain a solution C;
3) adding anhydrous ethanol, tween-80, 1, 2-propylene glycol and glycerol in the formula amount into the solution C prepared in the step 2) slowly in sequence, and stirring uniformly at a low speed to obtain a solution D;
4) and (3) adding distilled water into the solution D obtained in the step (3) to a sufficient amount, stirring at a low speed to be uniform, completely defoaming, and filling to obtain the shark peptide gel film coating agent.
Example 3
1. In embodiment 3 of the invention, a shark peptide gel coating agent comprises the following components in percentage by mass:
shark peptide, 3.0%; 8.0 percent of polyvinyl alcohol PVA-217; polyvinyl alcohol PVA-0588, 2.0%; 5.0% of glycerol; 1, 2-propanediol, 2.5%; chlorhexidine gluconate, 0.2%; absolute ethyl alcohol, 6.0%; tween-80, 0.2%; the balance of distilled water.
2. The preparation method of the shark peptide gel coating agent with the mixture ratio comprises the following steps:
1) soaking the polyvinyl alcohol PVA-217 and the polyvinyl alcohol PVA-0588 with the prescription amount in a proper amount of distilled water overnight, then fully dissolving in a water bath at about 80 ℃, stirring to be uniform to obtain a mucilage A, and naturally cooling;
2) fully dissolving the prescription amount of the megalosymus serpyllum in a proper amount of distilled water to obtain a solution B, mixing the solution B with the mucilage A prepared in the step 1), and uniformly stirring at a low speed at a temperature of 45 ℃ to obtain a solution C;
3) adding anhydrous ethanol, tween-80, 1, 2-propylene glycol and glycerol in the formula amount into the solution C prepared in the step 2) slowly in sequence, and stirring uniformly at a low speed to obtain a solution D;
4) adding a chlorhexidine gluconate solution with a prescription amount into the solution D in the step 3, adding distilled water to a sufficient amount, stirring at a low speed to be uniform, completely defoaming, and filling to obtain the shark peptide gel film coating agent.
Comparative example 1
The formulation of comparative example 1 is different from the formulation of example 2 in that comparative example 1 does not contain shark peptide, other component ratios are the same as those of example 2, and the preparation method is the same.
Film coating agent performance test
1) Film formation test
The shark peptide gel coating agents prepared in the above examples 1-3 and comparative example 1 were uniformly applied to the skin surface for film forming test.
The film forming effect of the film coating agent prepared in the comparative example 1 is poor, compared with the comparative example 1, the film coating agent prepared in the examples 1 to 3 can form a compact transparent film with a certain thickness and high elasticity and toughness at room temperature and normal body temperature for 8 to 15min, and meanwhile, the film coating agent also has the excellent performances of good flexibility, good adhesion and difficulty in falling off at joints; not only can effectively protect the wound surface and promote the wound healing, but also does not influence the beauty.
2) Test of bacteriostatic Property
Two strains of staphylococcus aureus and escherichia coli are selected, an operation method of 2.1.8.2 antibacterial ring test in disinfection technical specification (2002) is referred to, and antibacterial test detection is carried out on the shark peptide gel coating agent prepared in examples 2-3 and comparative example 1, and the results are shown in table 1:
TABLE 1
| Examples/comparative examples | Diameter of bacteriostatic circle (mm) of staphylococcus aureus | Diameter of bacteriostatic circle of Escherichia coli (mm) |
| Comparative example 1 | - | - |
| Example 2 | 13 | 11 |
| Example 3 | 25 | 25 |
Note: the above numerical values indicate the diameter (mm) of the zone of inhibition, and "-" indicates the zone of no inhibition.
According to the data results in the table above, the shark peptide gel coating agent provided by the invention has good bacteriostatic effect on staphylococcus aureus and escherichia coli. In addition, after the gel coating agent is exposed in the air for continuous culture for 7 days, the inhibition zone does not become small, and no mixed bacterial colony grows in the ring, which shows that the gel coating agent provided by the invention has a durable and broad-spectrum antibacterial effect.
Claims (10)
1. The shark peptide gel coating agent is characterized by comprising the following components in percentage by mass: 1.0-10.0% of shark peptide; 2.0 to 20.0 percent of gel film forming agent; 5.0 to 10.0 percent of penetration enhancer; 0.1 to 1.0 percent of antibacterial agent; 0 to 30.0 percent of absolute ethyl alcohol; 0.2 to 0.5 percent of tween-80; the balance of distilled water.
2. A shark peptide gel coat according to claim 1, wherein: the shark peptide is shark small molecular peptide, and the variety source is at least one of Chilossyllium platiosum, Mustellus griseus and Prionice glauca.
3. A shark peptide gel coat according to claim 1, wherein: the gel film forming agent is one or more of polyvinyl alcohol PVA-124, polyvinyl alcohol PVA-217, polyvinyl alcohol PVA-1788, polyvinyl alcohol PVA-0588, polyvidone, CMC-Na, polyhexamethylene glycol PEG-400, carbomer 940, Arabic gum, polyvinyl formal acetal and polyvinyl butyral.
4. A shark peptide gel coat according to claim 1, wherein: the penetration enhancer comprises 1, 2-propylene glycol and glycerol.
5. A shark peptide gel coat according to claim 4, wherein: the mass ratio of the 1, 2-propylene glycol to the glycerol is 1 (1-3).
6. A shark peptide gel coat according to claim 1, wherein: the antibacterial agent comprises one or two of chlorhexidine gluconate and nisin.
7. The shark peptide gel coat according to claim 1, comprising the following components in mass percent: 3.0 to 6.0 percent of shark peptide; 5.0 to 15.0 percent of gel film forming agent; 8.0 to 10.0 percent of penetration enhancer; 0.2 to 0.5 percent of antibacterial agent; 5.0 to 15.0 percent of absolute ethyl alcohol; tween-80, 0.2%; the balance of distilled water.
8. A preparation method of a shark peptide gel coating agent is characterized by comprising the following steps:
1) soaking the gel film forming agent in distilled water or ethanol solution overnight, then placing the gel film forming agent in water bath for full dissolution, stirring the gel film forming agent to be uniform and naturally cooling the gel film forming agent to obtain gel slurry A;
2) fully dissolving shark peptide in distilled water to obtain a solution B, mixing the solution B with the mucilage A prepared in the step 1), and uniformly stirring at a low speed to obtain a solution C;
3) respectively and sequentially adding absolute ethyl alcohol, tween-80 and a penetration enhancer into the solution C prepared in the step 2), and sufficiently and uniformly stirring at a low speed to obtain a solution D;
4) and (3) adding a compatible antibacterial agent into the solution D obtained in the step (3), adding distilled water to a sufficient amount, stirring at a low speed to be uniform, completely defoaming, and filling to obtain the shark peptide gel film coating agent.
9. The method of preparing a shark peptide gel coat according to claim 8, wherein the shark peptide gel coat comprises: the temperature of the water bath in the step 1) is 75-90 ℃.
10. The method of preparing a shark peptide gel coat according to claim 8, wherein the shark peptide gel coat comprises: the mixing temperature in the step 2) is 40-55 ℃.
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| CN112494422A (en) * | 2020-12-02 | 2021-03-16 | 湖北沃德利派生物科技有限公司 | Slow-release microemulsion temperature-sensitive antibacterial gel |
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| CN111676262A (en) * | 2020-07-10 | 2020-09-18 | 厦门肽王基因科技有限公司 | Shark composite short peptide with anti-inflammatory activity, preparation method and application |
| CN112494422A (en) * | 2020-12-02 | 2021-03-16 | 湖北沃德利派生物科技有限公司 | Slow-release microemulsion temperature-sensitive antibacterial gel |
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Application publication date: 20200324 |