CN113318061A

CN113318061A - A facial cream containing embryo extract and its preparation method

Info

Publication number: CN113318061A
Application number: CN202110666801.1A
Authority: CN
Inventors: 占震锋; 李庆静
Original assignee: Shanghai Nanbinjiang Cell Biotechnology Co ltd
Current assignee: Shanghai Nanbinjiang Cell Biotechnology Co ltd
Priority date: 2021-06-16
Filing date: 2021-06-16
Publication date: 2021-08-31

Abstract

The invention discloses a facial cream containing an embryo extract, which specifically comprises 3.5-5.4 parts of whitening particles, 0.5-1.2 parts of traditional Chinese medicine extracting solution, 0.03-0.08 part of chicken embryo extract, 0.3-0.6 part of sodium stearate and 0.05-0.06 part of dipotassium glycyrrhizinate according to parts by weight, and belongs to the technical field of cosmetics. The raw materials of the facial cream are added with radix angelicae aqueous extract stock solution and poria cocos absolute ethyl alcohol extract stock solution, so that tyrosinase activity is inhibited, melanin deposition is well prevented, melanin generation is reduced, and whitening efficacy is achieved.

Description

A facial cream containing embryo extract and its preparation method

Technical Field

The invention relates to the technical field of cosmetics, in particular to a facial cream containing an embryo extract and a preparation method thereof.

Background

With the improvement of civilization of society and the continuous improvement of living standard and quality of people, people pay more attention to the quality and safety of cosmetic raw materials, and expect to use green natural raw materials which have skin-care and beauty-care effects and are harmless to human health. The material used for cosmetics is characterized by good stability and no allergic reaction to human skin. The traditional face cream cosmetics have poor whitening effect, and the effect of components in the face cream is poor, so the invention provides the face cream containing the embryo extract.

Disclosure of Invention

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides the cream containing the embryo extract and the preparation method thereof, and solves the problems that the whitening effect of the traditional cream cosmetics is poor, and the effect of components in the cream is poor.

(II) technical scheme

In order to achieve the purpose, the invention is realized by the following technical scheme: a facial cream containing embryo extract comprises (by weight parts) whitening particles 3.5-5.4, Chinese medicinal extractive solution 0.5-1.2, chicken embryo extract 0.03-0.08, sodium stearate 0.3-0.6, and dipotassium glycyrrhizinate 0.05-0.06;

wherein the whitening particles are prepared by the following steps:

step one, weighing the yuenkanin polysaccharide and the bamboo leaf flavone respectively at room temperature, dissolving in distilled water, and preparing a yuenkanin polysaccharide-bamboo leaf flavone solution with the concentration of 3 mg/mL;

step two, dripping the epsilon-polylysine solution into the yuenkanin polysaccharide-bamboo leaf flavonoid solution by using a constant flow pump, and simultaneously stirring at a low speed by using a magnetic stirrer to obtain whitening particles;

the traditional Chinese medicine extracting solution comprises radix angelicae extracting stock solution and chinaroot greenbrier rhizome extracting stock solution, the mass concentration of the radix angelicae extracting stock solution and the chinaroot greenbrier rhizome extracting stock solution is 1.0g/mL, and the volume ratio of the radix angelicae extracting stock solution to the chinaroot greenbrier rhizome extracting stock solution is 2: 3.

Preferably, in the first step, the dosage ratio of the yuenkanin polysaccharide to the bamboo leaf flavonoid is 1.5 g: 0.1 g.

Preferably, in the second step, the dosage ratio of the epsilon-polylysine solution to the yuenkanin-bamboo leaf flavonoid solution is 20 mL: 95mL, setting the rotating speed of the constant flow pump at 20r/min and the rotating speed of the magnetic stirrer at 180 r/min.

Preferably, the radix angelicae extraction stock solution is prepared by the following method: crushing the angelica dahurica traditional Chinese medicinal materials, and sieving the crushed angelica dahurica traditional Chinese medicinal materials with a 40-mesh sieve according to the dosage ratio of 10 g: weighing 100mL of radix angelicae dahuricae powder and distilled water, putting into a flask, stirring and extracting for 8h under the water bath heating condition of 35 ℃, filtering and centrifuging, and concentrating supernatant to 10mL to obtain radix angelicae dahuricae extract stock solution.

Preferably, the white poria cocos extraction stock solution is prepared by the following method: crushing the white poria cocos traditional Chinese medicinal materials, and sieving the crushed white poria cocos traditional Chinese medicinal materials with a 40-mesh sieve according to the dosage ratio of 10 g: weighing 100mL of white poria coarse powder and 70% volume fraction ethanol, putting into a flask, carrying out ultrasonic extraction at 200Hz for 20min under the water bath heating condition at 25 ℃, carrying out primary filtration, adding 70% volume fraction ethanol into filter residues in the same proportion, carrying out ultrasonic extraction at 200Hz for 20min again, carrying out secondary filtration, combining filtrates obtained by the two-time filtration, concentrating to 100mL, centrifuging to obtain a supernatant, and concentrating under reduced pressure to 10mL to obtain a white poria extract stock solution.

Preferably, the preparation method of the facial cream containing the embryo extract comprises the following steps:

the first step is as follows: putting sodium stearate and dipotassium glycyrrhizinate into a beaker, stirring and heating in a water bath kettle at 80 ℃ for half an hour to completely dissolve lipid, and marking as phase A;

secondly, putting the traditional Chinese medicine extracting solution, the chicken embryo extract and the whitening particles into another beaker, and stirring and dispersing uniformly in a water bath kettle at 80 ℃ to be marked as a phase B;

and thirdly, stirring the phase A by using a mechanical stirrer, adding the phase B into the phase A, keeping the temperature at 80 ℃, stirring for 10 minutes, and homogenizing for 3 minutes at 10000rpm/min by using a digital display high-speed shearing dispersion machine to obtain the cream containing the embryo extract.

Preferably, the sodium stearate is sodium monostearate glycerol provided by Anhui Zhonghong bioengineering limited, and the dipotassium glycyrrhizinate is dipotassium glycyrrhizinate provided by Hubei Jiulong chemical company limited.

Preferably, the chicken embryo extract is prepared by the method in Chinese patent CN 106138101.

(III) advantageous effects

Compared with the prior art, the facial cream containing the embryo extract and the preparation method thereof have the following beneficial effects:

the whitening cream is characterized in that an angelica dahurica water extraction stock solution and a poria cocos absolute ethyl alcohol extraction stock solution are added into a raw material of the cream, the angelica dahurica water extraction stock solution is a main active substance which plays a role in whitening, and can effectively inhibit phototoxicity, tyrosinase is a decisive rate-limiting enzyme in a melanin cell synthesis melanin process, the catalysis effect of the tyrosinase is mainly generated in two reactions of converting tyrosine into dopa and converting dopa into dopaquinone, the tyrosinase activity is inhibited by the angelica dahurica water extraction stock solution and the poria cocos absolute ethyl alcohol extraction stock solution, the inhibition rate of the poria cocos absolute ethyl alcohol extraction solution on tyrosinase is 91.7%, the better melanin deposition prevention effect is achieved, and the generation of melanin is reduced to achieve the whitening effect;

cationic polymerization polypeptide epsilon-polylysine is taken as an ionic cross-linking agent, and is cross-linked with negatively charged turnip polysaccharide and bamboo leaf flavone ions to form bamboo leaf flavone whitening particles with uniform and stable particle size, the whitening particles have stronger inhibition on the synthesis of tyrosinase activity, and the inhibition rate on the tyrosinase activity in cells is 71.98% when the concentration is 100ug/mL, so that the whitening effect is effectively achieved, the safety is better, and in addition, the bamboo leaf flavone has stronger oxidation resistance, so that the whitening effect can be better shown; the bamboo leaf flavone can inhibit the synthesis of melanin by inhibiting the activity of tyrosinase and also can inhibit the generation of melanin by blocking the transfer of melanin granules from melanocytes to horny layer;

by applying the chicken embryo extract in the raw materials of the face cream and adopting the method in Chinese patent CN106138101 to prepare the chicken embryo extract, various bioactive substances in chicken embryos are effectively retained, various nutrient substances are effectively extracted, the bioavailability is high, and the face cream product containing the chicken embryo extract has certain effect of replenishing qi and blood.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1:

the whitening particles are prepared by the following steps:

step one, respectively weighing 1.5g of yuenkanin and 0.1g of bamboo leaf flavone and dissolving in distilled water at room temperature to prepare a yuenkanin-bamboo leaf flavone solution with the concentration of 3 mg/mL;

and step two, dripping 20mL of epsilon-polylysine solution into 95mL of the yuenkanin polysaccharide-bamboo leaf flavonoid solution by using a constant flow pump at the rotating speed of 20r/min, and stirring by using a magnetic stirrer at the rotating speed of 180r/min to obtain the whitening particles.

Example 2:

the radix angelicae extraction stock solution is prepared by the following method:

crushing the angelica dahurica traditional Chinese medicinal materials, and sieving the crushed angelica dahurica traditional Chinese medicinal materials with a 40-mesh sieve according to the dosage ratio of 10 g: weighing 100mL of radix angelicae dahuricae powder and distilled water, putting into a flask, stirring and extracting for 8h under the water bath heating condition of 35 ℃, filtering and centrifuging, and concentrating supernatant to 10mL to obtain radix angelicae dahuricae extract stock solution.

Example 3:

the white poria cocos extraction stock solution is prepared by the following method:

crushing the white poria cocos traditional Chinese medicinal materials, and sieving the crushed white poria cocos traditional Chinese medicinal materials with a 40-mesh sieve according to the dosage ratio of 10 g: weighing 100mL of white poria coarse powder and 70% volume fraction ethanol, putting into a flask, carrying out ultrasonic extraction at 200Hz for 20min under the water bath heating condition at 25 ℃, carrying out primary filtration, adding 70% volume fraction ethanol into filter residues in the same proportion, carrying out ultrasonic extraction at 200Hz for 20min again, carrying out secondary filtration, combining filtrates obtained by the two-time filtration, concentrating to 100mL, centrifuging to obtain a supernatant, and concentrating under reduced pressure to 10mL to obtain a white poria extract stock solution.

Example 4:

a method for preparing facial cream containing embryo extract comprises the following steps:

the first step is as follows: putting 6g sodium stearate and 1g dipotassium glycyrrhizinate into a beaker, stirring and heating in a water bath kettle at 80 ℃ for half an hour to completely dissolve lipid, and marking as phase A;

step two, preparing 10mL of traditional Chinese medicine extracting solution, 0.6mL of chicken embryo extract and 7g of whitening particles in the embodiment 1 from the radix angelicae extracting stock solution in the embodiment 2 and the poria cocos extracting stock solution in the embodiment 3 according to the volume ratio of 2:3, putting the two into another beaker, and stirring and dispersing the two in a water bath kettle at 80 ℃ uniformly, wherein the mixture is marked as phase B;

Example 5:

this example is different from example 4 in that no herb extract was added in the second step.

Example 6:

this example differs from example 4 in that no whitening particles are added in the second step.

Comparative example 1:

the comparative example adopts a commercial OLAY yulan oil multi-effect repair face cream.

Comparative experiment:

selecting the creams prepared in the examples 4-6 and the comparative example 1 to carry out tyrosinase inhibition rate test experiments, and respectively preparing PBS (phosphate buffer solution) with the pH value of 6.8, 1.0mg/mL L-tyrosinase solution and 0.1mg/mL tyrosinase solution; adding samples according to the following mixture ratio, wherein a test sample 1 is measured: 2.0mL PBS +0.5mL tyrosinase solution +0.5 mL-tyrosinase solution; test sample 2: 2.5mL PBS +0.5mL tyrosinase solution; test sample 3: 0.5mL of sample solution +1.5mL of PBS +0.5mL of tyrosinase solution +0.5mL of tyrosinase-solution; test sample 4: 0.5mL sample solution +2.0mL PBS +0.5mL tyrosinase solution; mixing the test samples, mixing 1-4 kinds of test samples, placing in 37 deg.C water bath for 30min, immediately taking out, and measuring absorbance at 475nm (A1-Aa);

according to the formula

Calculating the tyrosinase inhibition rate (IC) of the cream₅₀)，IC₅₀The lower the value of (A) indicates a greater inhibitory activity of the cream on tyrosinase, where A1-A4 is the absorbance value at 475nm of test samples 1-4, and the results are given in the following table:

TABLE 1

	Example 4	Example 5	Comparative example 6	Comparative example 1
					IC₅₀(g·L^-1)	0.65	0.71	0.98	1.35

As can be seen from Table 1, the IC of example 4₅₀The values are significantly lower than the IC of examples 5 to 6 and comparative example 1₅₀The reason is that the traditional Chinese medicine extracting solution and the whitening particles are used in the embodiment 4, and the traditional Chinese medicine extracting solution and the whitening particles have higher inhibition rate on tyrosinase, so that the face cream product has better whitening effect.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A facial cream containing embryo extract, which is characterized in that: the raw materials specifically comprise 3.5 to 5.4 parts of whitening particles, 0.5 to 1.2 parts of traditional Chinese medicine extracting solution, 0.03 to 0.08 part of chicken embryo extract, 0.3 to 0.6 part of sodium stearate and 0.05 to 0.06 part of dipotassium glycyrrhizinate by weight;

wherein the whitening particles are prepared by the following steps:

the traditional Chinese medicine extracting solution comprises radix angelicae extracting stock solution and chinaroot greenbrier rhizome extracting stock solution, and the mass concentration of the radix angelicae extracting stock solution and the chinaroot greenbrier rhizome extracting stock solution is 1.0 g/mL.

2. The cream containing embryo extract according to claim 1, wherein: in the first step, the dosage ratio of the yuenkanin polysaccharide to the bamboo leaf flavone is 1.5 g: 0.1 g.

3. The cream containing embryo extract according to claim 1, wherein: in the second step, the dosage ratio of the epsilon-polylysine solution to the yuenkanin polysaccharide-bamboo leaf flavonoid solution is 20 mL: 95mL, setting the rotating speed of the constant flow pump at 20r/min and the rotating speed of the magnetic stirrer at 180 r/min.

4. The cream containing embryo extract according to claim 1, wherein: the radix angelicae extraction stock solution is prepared by the following method: crushing the angelica dahurica traditional Chinese medicinal materials, and sieving the crushed angelica dahurica traditional Chinese medicinal materials with a 40-mesh sieve according to the dosage ratio of 10 g: weighing 100mL of radix angelicae dahuricae powder and distilled water, putting into a flask, stirring and extracting for 8h under the water bath heating condition of 35 ℃, filtering and centrifuging, and concentrating supernatant to 10mL to obtain radix angelicae dahuricae extract stock solution.

5. The cream containing embryo extract according to claim 1, wherein: the white poria cocos extraction stock solution is prepared by the following method: crushing the white poria cocos traditional Chinese medicinal materials, and sieving the crushed white poria cocos traditional Chinese medicinal materials with a 40-mesh sieve according to the dosage ratio of 10 g: weighing 100mL of white poria coarse powder and 70% volume fraction ethanol, putting into a flask, carrying out ultrasonic extraction for 20min under the water bath heating condition of 25 ℃, carrying out primary filtration, adding 70% volume fraction ethanol into filter residues according to the same proportion, carrying out ultrasonic extraction for 20min again, carrying out secondary filtration, combining filtrates obtained by two times of filtration, concentrating to 100mL, centrifuging to obtain a supernatant, and concentrating under reduced pressure to 10mL to obtain a white poria extract stock solution.

6. The method for preparing facial cream containing embryo extract as claimed in claim 1, wherein the method comprises the steps of: the method comprises the following steps:

and thirdly, stirring the phase A by using a mechanical stirrer, simultaneously adding the phase B into the phase A, keeping the temperature at 80 ℃, stirring for 10 minutes, and homogenizing for 3 minutes to obtain the cream containing the embryo extract.